AlfalfaGEDB | MS.gene38527

M. sativa cultivar XinJiangDaYe / MS.gene38527

KEGG: K20240
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016746 GO:0016747
PFAM: PF02458.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38527.t1	RHN67803.1	90.4	467	42	2	1	467	47	510	3.00E-241	844.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38527.t1	Q8GYW8	38.5	465	258	13	8	464	11	455	2.7e-73	277.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38527.t1	A0A396ITJ2	90.4	467	42	2	1	467	47	510	2.2e-241	844.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene011837	MS.gene38527	PPI
MS.gene95242	MS.gene38527	PPI
MS.gene38527	MS.gene87577	PPI
MS.gene32936	MS.gene38527	PPI
MS.gene90496	MS.gene38527	PPI
MS.gene32938	MS.gene38527	PPI
MS.gene000763	MS.gene38527	PPI
MS.gene38362	MS.gene38527	PPI
MS.gene28251	MS.gene38527	PPI
MS.gene011801	MS.gene38527	PPI
MS.gene000764	MS.gene38527	PPI
MS.gene38527	MS.gene87191	PPI
MS.gene38977	MS.gene38527	PPI
MS.gene90491	MS.gene38527	PPI
MS.gene38527	MS.gene87496	PPI
MS.gene38527	MS.gene070019	PPI
MS.gene87532	MS.gene38527	PPI
MS.gene99223	MS.gene38527	PPI
MS.gene46265	MS.gene38527	PPI
MS.gene063868	MS.gene38527	PPI
MS.gene38703	MS.gene38527	PPI
MS.gene066658	MS.gene38527	PPI
MS.gene066634	MS.gene38527	PPI
MS.gene38527	MS.gene56799	PPI
MS.gene99221	MS.gene38527	PPI
MS.gene38363	MS.gene38527	PPI
MS.gene039597	MS.gene38527	PPI
MS.gene32934	MS.gene38527	PPI
MS.gene063865	MS.gene38527	PPI
MS.gene007831	MS.gene38527	PPI
MS.gene38527	MS.gene38702	PPI
MS.gene56796	MS.gene38527	PPI
MS.gene069767	MS.gene38527	PPI
MS.gene004218	MS.gene38527	PPI
MS.gene011796	MS.gene38527	PPI
MS.gene87537	MS.gene38527	PPI
MS.gene99222	MS.gene38527	PPI
MS.gene38527	MS.gene50384	PPI
MS.gene90493	MS.gene38527	PPI
MS.gene032349	MS.gene38527	PPI
MS.gene38704	MS.gene38527	PPI
MS.gene013787	MS.gene38527	PPI
MS.gene066656	MS.gene38527	PPI
MS.gene066660	MS.gene38527	PPI
MS.gene38527	MS.gene012961	PPI
MS.gene069321	MS.gene38527	PPI
MS.gene38527	MS.gene32933	PPI
MS.gene38527	MS.gene064101	PPI
MS.gene38361	MS.gene38527	PPI
MS.gene03202	MS.gene38527	PPI
MS.gene38706	MS.gene38527	PPI
MS.gene38527	MS.gene50356	PPI
MS.gene38527	MS.gene56797	PPI
MS.gene046246	MS.gene38527	PPI
MS.gene38700	MS.gene38527	PPI
MS.gene012960	MS.gene38527	PPI
MS.gene38365	MS.gene38527	PPI
MS.gene38527	MS.gene38359	PPI
MS.gene38527	MS.gene016532	PPI
MS.gene50355	MS.gene38527	PPI
MS.gene011798	MS.gene38527	PPI
MS.gene38527	MS.gene066701	PPI
MS.gene012965	MS.gene38527	PPI
MS.gene32261	MS.gene38527	PPI
MS.gene012957	MS.gene38527	PPI
MS.gene99219	MS.gene38527	PPI
MS.gene38527	MS.gene011799	PPI
MS.gene38527	MS.gene50420	PPI
MS.gene38527	MS.gene38699	PPI
MS.gene56795	MS.gene38527	PPI
MS.gene066657	MS.gene38527	PPI
MS.gene56798	MS.gene38527	PPI
MS.gene002544	MS.gene38527	PPI
MS.gene78818	MS.gene38527	PPI
MS.gene38527	MS.gene87536	PPI
MS.gene38701	MS.gene38527	PPI
MS.gene38527	MS.gene99218	PPI
MS.gene38527	MS.gene012959	PPI
MS.gene50379	MS.gene38527	PPI
MS.gene38527	MS.gene016477	PPI
MS.gene90492	MS.gene38527	PPI
MS.gene38527	MS.gene02573	PPI
MS.gene50382	MS.gene38527	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38527.t1	MTR_3g465910	85.714	497	59	4	1	490	1	492	0.0	879
MS.gene38527.t1	MTR_3g465930	87.339	466	59	0	1	466	1	466	0.0	865
MS.gene38527.t1	MTR_3g465950	79.778	450	79	3	1	449	1	439	0.0	730
MS.gene38527.t1	MTR_3g465980	69.099	466	136	5	1	464	1	460	0.0	672
MS.gene38527.t1	MTR_3g465870	71.855	469	87	6	1	466	1	427	0.0	662
MS.gene38527.t1	MTR_3g465990	66.524	466	148	5	1	464	1	460	0.0	656
MS.gene38527.t1	MTR_3g465880	66.314	472	146	8	1	464	1	467	0.0	635
MS.gene38527.t1	MTR_6g053810	60.256	468	175	9	1	462	1	463	0.0	555
MS.gene38527.t1	MTR_4g055170	48.608	467	214	11	7	464	9	458	1.97e-144	424
MS.gene38527.t1	MTR_2g015430	30.719	459	292	15	8	462	2	438	7.45e-62	210
MS.gene38527.t1	MTR_7g027175	29.957	464	275	17	3	456	10	433	7.65e-42	155
MS.gene38527.t1	MTR_8g096530	27.706	462	285	15	8	459	5	427	5.02e-39	148
MS.gene38527.t1	MTR_1g053315	28.936	470	283	19	18	466	26	465	2.91e-38	146
MS.gene38527.t1	MTR_1g040175	30.145	345	219	9	10	348	9	337	4.90e-38	145
MS.gene38527.t1	MTR_3g098980	28.384	458	280	16	8	456	12	430	1.27e-36	141
MS.gene38527.t1	MTR_1g081660	26.030	461	305	17	8	461	16	447	1.04e-35	139
MS.gene38527.t1	MTR_3g071320	29.238	407	247	14	10	400	5	386	5.28e-35	137
MS.gene38527.t1	MTR_7g110610	31.642	335	171	9	17	348	12	291	6.65e-35	135
MS.gene38527.t1	MTR_1g040215	28.571	350	224	9	7	348	6	337	1.91e-34	135
MS.gene38527.t1	MTR_5g076700	26.209	393	263	12	17	403	17	388	4.63e-32	129
MS.gene38527.t1	MTR_7g114520	28.788	396	254	14	17	405	18	392	5.34e-32	129
MS.gene38527.t1	MTR_7g114550	28.358	335	225	7	17	348	17	339	7.36e-31	125
MS.gene38527.t1	MTR_7g114470	26.515	396	263	13	17	405	18	392	1.34e-29	122
MS.gene38527.t1	MTR_7g114490	27.020	396	259	13	17	405	17	389	1.43e-29	122
MS.gene38527.t1	MTR_7g114560	28.274	336	224	9	17	348	17	339	5.34e-29	120
MS.gene38527.t1	MTR_8g075610	27.901	362	229	11	17	369	1	339	3.30e-28	117
MS.gene38527.t1	MTR_3g112180	26.456	412	228	15	18	400	20	385	4.42e-28	117
MS.gene38527.t1	MTR_2g105330	26.238	404	260	13	10	400	3	381	1.86e-27	115
MS.gene38527.t1	MTR_2g064495	25.435	460	295	15	10	456	3	427	1.37e-26	112
MS.gene38527.t1	MTR_4g007540	26.355	406	258	14	10	400	3	382	1.15e-25	110
MS.gene38527.t1	MTR_1g040320	24.891	458	281	16	7	461	6	403	3.39e-24	105
MS.gene38527.t1	MTR_1g040315	27.160	324	206	8	4	319	3	304	3.65e-24	105
MS.gene38527.t1	MTR_3g049150	26.371	383	238	16	18	380	10	368	6.82e-24	105
MS.gene38527.t1	MTR_4g127650	29.155	343	210	12	18	346	18	341	9.77e-24	104
MS.gene38527.t1	MTR_4g097090	27.458	295	181	8	69	347	65	342	2.76e-22	100
MS.gene38527.t1	MTR_3g026950	23.679	473	288	17	18	455	10	444	2.09e-21	97.4
MS.gene38527.t1	MTR_0502s0010	29.600	250	154	9	110	349	2	239	6.29e-21	92.4
MS.gene38527.t1	MTR_1g040195	26.566	399	246	15	78	461	48	414	6.77e-21	95.9
MS.gene38527.t1	MTR_7g085930	26.923	338	207	13	57	369	47	369	9.55e-21	95.5
MS.gene38527.t1	MTR_7g086000	28.213	319	200	12	18	321	12	316	1.98e-20	94.7
MS.gene38527.t1	MTR_3g102020	27.119	295	189	8	52	334	52	332	2.32e-18	88.2
MS.gene38527.t1	MTR_4g013050	24.668	377	229	12	49	410	32	368	2.64e-18	87.8
MS.gene38527.t1	MTR_3g101960	27.063	303	181	8	51	334	50	331	5.81e-17	84.0
MS.gene38527.t1	MTR_0042s0190	29.508	183	113	5	172	348	1	173	7.15e-17	81.6
MS.gene38527.t1	MTR_3g102010	26.882	279	171	8	61	320	54	318	1.15e-16	82.8
MS.gene38527.t1	MTR_7g084940	26.471	408	243	17	8	395	14	384	1.27e-16	82.8
MS.gene38527.t1	MTR_4g022720	26.471	272	186	6	69	334	55	318	2.44e-15	78.6
MS.gene38527.t1	MTR_7g114510	26.966	267	173	10	154	411	8	261	4.23e-15	77.0
MS.gene38527.t1	MTR_7g056087	24.755	408	258	16	9	394	3	383	4.44e-15	77.8
MS.gene38527.t1	MTR_3g101970	26.510	298	179	10	52	337	52	321	2.06e-14	75.9
MS.gene38527.t1	MTR_3g101930	25.091	275	184	6	70	332	71	335	4.54e-14	74.7
MS.gene38527.t1	MTR_3g101900	25.818	275	184	7	69	334	66	329	9.19e-14	73.9
MS.gene38527.t1	MTR_3g450710	23.077	325	207	11	69	368	65	371	2.84e-12	69.3
MS.gene38527.t1	MTR_8g041770	25.830	271	181	8	69	332	64	321	1.06e-11	67.4
MS.gene38527.t1	MTR_4g022670	26.042	288	184	11	69	339	55	330	5.13e-11	65.1
MS.gene38527.t1	MTR_7g084910	26.765	340	210	12	8	331	15	331	5.39e-11	65.1
MS.gene38527.t1	MTR_3g450690	23.292	322	207	11	69	368	72	375	5.58e-11	65.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38527.t1	AT5G07080	38.806	469	242	12	8	462	12	449	2.15e-94	295
MS.gene38527.t1	AT2G25150	38.495	465	258	13	8	464	11	455	4.71e-90	284
MS.gene38527.t1	AT2G23510	38.178	461	257	13	8	461	11	450	7.04e-87	276
MS.gene38527.t1	AT3G47170	38.347	472	253	17	10	461	12	465	3.10e-85	272
MS.gene38527.t1	AT1G28680	35.217	460	272	15	6	459	2	441	1.76e-74	243
MS.gene38527.t1	AT1G28680	36.898	374	215	13	6	373	2	360	4.66e-62	209
MS.gene38527.t1	AT3G48720	29.565	460	255	20	17	456	16	426	3.97e-43	159
MS.gene38527.t1	AT5G41040	28.758	459	282	15	8	457	15	437	8.11e-43	159
MS.gene38527.t1	AT5G41040	28.758	459	282	15	8	457	31	453	1.05e-42	159
MS.gene38527.t1	AT3G62160	31.472	394	225	14	16	400	11	368	3.48e-42	156
MS.gene38527.t1	AT3G03480	28.322	459	299	15	7	461	16	448	2.38e-38	146
MS.gene38527.t1	AT5G63560	29.902	408	243	17	8	405	7	381	1.10e-34	135
MS.gene38527.t1	AT5G17540	29.050	358	231	11	7	357	6	347	9.16e-33	130
MS.gene38527.t1	AT1G03390	27.208	419	263	14	20	423	32	423	4.05e-32	129
MS.gene38527.t1	AT1G27620	27.873	409	239	15	11	401	10	380	1.12e-30	124
MS.gene38527.t1	AT2G40230	27.708	397	262	12	10	400	5	382	2.82e-30	123
MS.gene38527.t1	AT5G48930	27.467	375	234	12	8	369	1	350	1.43e-28	118
MS.gene38527.t1	AT2G19070	28.017	464	277	20	18	455	12	444	3.35e-24	105
MS.gene38527.t1	AT5G57840	26.984	378	234	16	8	369	1	352	6.95e-21	95.9
MS.gene38527.t1	AT4G31910	26.456	412	254	20	28	412	29	418	3.50e-18	87.8
MS.gene38527.t1	AT1G24430	28.617	311	178	17	17	318	12	287	5.37e-18	87.0
MS.gene38527.t1	AT1G65450	27.671	365	224	15	46	395	53	392	7.34e-18	86.7
MS.gene38527.t1	AT5G47950	32.512	203	104	10	7	199	3	182	9.37e-13	70.9
MS.gene38527.t1	AT5G23940	27.652	264	165	9	70	318	66	318	1.18e-12	70.5
MS.gene38527.t1	AT4G15390	23.967	363	219	16	4	348	2	325	1.82e-11	66.6
MS.gene38527.t1	AT4G15390	23.967	363	219	16	4	348	2	325	1.82e-11	66.6
MS.gene38527.t1	AT4G29250	27.493	371	218	18	18	367	22	362	2.82e-11	66.2
MS.gene38527.t1	AT3G26040	24.275	276	176	11	17	286	10	258	3.68e-11	65.9

Find 89 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATACCAACGGTACTGAATT+TGG	0.155661	3.4:-61563705	MS.gene38527:CDS
GGGATGAAATTGAAGCTTTA+AGG	0.166973	3.4:-61562639	MS.gene38527:intron
TTTCAGGAAAATTTAGATTT+TGG	0.167927	3.4:-61562826	MS.gene38527:CDS
AAGTTACCCTTTGGTGTTAA+TGG	0.185564	3.4:-61563565	MS.gene38527:CDS
ACTTTACCTAAAGGGTATTA+TGG	0.240183	3.4:-61563075	MS.gene38527:CDS
TCATGATCATCAAGTTTATT+AGG	0.262038	3.4:+61562727	None:intergenic
AGTTAGGGCAATGTTATTAT+TGG	0.269128	3.4:+61564964	None:intergenic
CTTTGTGGTGGTTTCACAAT+TGG	0.270838	3.4:-61563531	MS.gene38527:CDS
ATACCAACGGTACTGAATTT+GGG	0.304721	3.4:-61563704	MS.gene38527:CDS
CACAAACTCAATAAACTCTT+TGG	0.322202	3.4:-61562932	MS.gene38527:CDS
CACAGAAATTGCAAAACATT+TGG	0.334670	3.4:-61563616	MS.gene38527:CDS
GGATGGTTTGAGCATGGAAT+TGG	0.352453	3.4:-61562896	MS.gene38527:CDS
GAATTGCCATAATACCCTTT+AGG	0.371067	3.4:+61563069	None:intergenic
TCTAACGGAGTGGAAACATT+TGG	0.373940	3.4:-61562851	MS.gene38527:CDS
CCTTTCTTCTCTTCATTACC+TGG	0.381983	3.4:-61563649	MS.gene38527:CDS
TTGCTGCTATGCCTATGTTC+AGG	0.383464	3.4:-61562660	MS.gene38527:CDS
CTAACGGAGTGGAAACATTT+GGG	0.386174	3.4:-61562850	MS.gene38527:CDS
CTAACTCCTCCATCCATTGA+TGG	0.386304	3.4:+61562703	None:intergenic
AAATTCAGTACCGTTGGTAT+TGG	0.387756	3.4:+61563707	None:intergenic
CTATCAACACTTGACAATAA+AGG	0.389671	3.4:-61564989	MS.gene38527:CDS
AACATAGGCATAGCAGCAAC+AGG	0.398229	3.4:+61562664	None:intergenic
TACATGACAAGGGTTGAAAC+TGG	0.406337	3.4:+61564898	None:intergenic
TGGTATCAGACGAAACTTGA+TGG	0.415157	3.4:-61563106	MS.gene38527:CDS
ATTATGATACTTTACCTAAA+GGG	0.416811	3.4:-61563083	MS.gene38527:CDS
CATCAATGGATGGAGGAGTT+AGG	0.420940	3.4:-61562702	MS.gene38527:CDS
CATGCTATTTGTGACGGATT+TGG	0.423939	3.4:-61563495	MS.gene38527:CDS
TCTTGAAACTGAATCATCTC+AGG	0.429330	3.4:+61561107	None:intergenic
AGTCTCTTTAATCAGCTTAA+CGG	0.431509	3.4:+61562984	None:intergenic
ATCGGTATACATGAACAGTT+AGG	0.437646	3.4:+61564948	None:intergenic
CAAGGATAAAAGTTACCCTT+TGG	0.446508	3.4:-61563574	MS.gene38527:CDS
TGCTGCTATGCCTATGTTCA+GGG	0.448433	3.4:-61562659	MS.gene38527:CDS
GGTGGTTTCACAATTGGAAT+GGG	0.460166	3.4:-61563525	MS.gene38527:CDS
GATTATGATACTTTACCTAA+AGG	0.466862	3.4:-61563084	MS.gene38527:CDS
TCCATGGGACATTGTGGAAA+TGG	0.468394	3.4:+61563363	None:intergenic
TCCCACACAGGTTTCACTGA+TGG	0.471401	3.4:+61563420	None:intergenic
TGGTGGTTTCACAATTGGAA+TGG	0.478781	3.4:-61563526	MS.gene38527:CDS
GCAAGCATCTTGCATGGTGC+AGG	0.485601	3.4:+61562778	None:intergenic
TTGTCACCATTAACACCAAA+GGG	0.486355	3.4:+61563559	None:intergenic
GATAGAGAAAGAATACAAGA+AGG	0.498935	3.4:+61565007	None:intergenic
AGAAAGAATACAAGAAGGGA+TGG	0.499295	3.4:+61565012	None:intergenic
TGAATGAAAATGAAAACATA+AGG	0.501906	3.4:-61563227	MS.gene38527:CDS
AAAACTAGTATACCTGCTAA+AGG	0.502972	3.4:+61564836	None:intergenic
TTTCTGTGTTAGTATTGTCC+AGG	0.512010	3.4:+61563631	None:intergenic
AGTCAGAAGACAAGAAGAGC+TGG	0.518629	3.4:-61560973	MS.gene38527:CDS
ATAGAGAAAGAATACAAGAA+GGG	0.521290	3.4:+61565008	None:intergenic
TCAATTTCATCCCTGAACAT+AGG	0.521815	3.4:+61562649	None:intergenic
AATAAGTTCTTTGTGAAACC+TGG	0.522984	3.4:-61561155	MS.gene38527:CDS
AGCAACCCTAAAGATTGAAA+AGG	0.526012	3.4:-61565065	MS.gene38527:CDS
TCTTGATATTCCTTCTCCCA+AGG	0.526970	3.4:-61563592	MS.gene38527:CDS
AGCTTAACGGTTTCATAGAG+TGG	0.529369	3.4:+61562997	None:intergenic
CCAGGTAATGAAGAGAAGAA+AGG	0.533118	3.4:+61563649	None:intergenic
TTTCAAGACAAAGTGGAAGC+TGG	0.540427	3.4:-61561092	MS.gene38527:CDS
TACCAACGGTACTGAATTTG+GGG	0.542547	3.4:-61563703	MS.gene38527:CDS
CAGGTAATGAAGAGAAGAAA+GGG	0.549845	3.4:+61563650	None:intergenic
GCAGTTGTTGAACTTGCAAG+TGG	0.559166	3.4:-61563453	MS.gene38527:CDS
TGATGATCATGATCCATCAA+TGG	0.560359	3.4:-61562716	MS.gene38527:CDS
GGAAGCTAGTGGTGCAGTAA+CGG	0.562555	3.4:-61562875	MS.gene38527:CDS
TTTGTCACCATTAACACCAA+AGG	0.568610	3.4:+61563558	None:intergenic
GTGACAAAGTTTCTTTGTGG+TGG	0.569090	3.4:-61563543	MS.gene38527:CDS
GATTCAGTTTCAAGACAAAG+TGG	0.572174	3.4:-61561099	MS.gene38527:CDS
TTTCTGTCCATGGGACATTG+TGG	0.573049	3.4:+61563357	None:intergenic
ATGTCACATGCTATTTGTGA+CGG	0.574392	3.4:-61563501	MS.gene38527:CDS
AAGTGCTTGCAGGTTATGTG+TGG	0.575180	3.4:-61563191	MS.gene38527:CDS
TTGGAGACGAATCAAGAGGA+TGG	0.583764	3.4:-61562913	MS.gene38527:CDS
TCAAGAGGATGGTTTGAGCA+TGG	0.589110	3.4:-61562902	MS.gene38527:CDS
TGGTGCAGTAACGGTTCTAA+CGG	0.589287	3.4:-61562866	MS.gene38527:CDS
TCGGTATACATGAACAGTTA+GGG	0.590541	3.4:+61564949	None:intergenic
ACCATTTCCACAATGTCCCA+TGG	0.598120	3.4:-61563364	MS.gene38527:CDS
AACCCCAAATTCAGTACCGT+TGG	0.605483	3.4:+61563701	None:intergenic
ATGGTGACAAAGTTTCTTTG+TGG	0.609696	3.4:-61563546	MS.gene38527:CDS
TGAGAAAATACACGTATCGA+TGG	0.615109	3.4:+61562753	None:intergenic
GATCATGATCCATCAATGGA+TGG	0.615987	3.4:-61562712	MS.gene38527:CDS
CATGAATTGCTGATGATGAT+CGG	0.619995	3.4:+61564930	None:intergenic
ATTCAAAGAAGCGCTGTCGA+AGG	0.626139	3.4:-61564876	MS.gene38527:CDS
GCTTCTTTGAATACATGACA+AGG	0.630468	3.4:+61564887	None:intergenic
CTTCTTTGAATACATGACAA+GGG	0.630974	3.4:+61564888	None:intergenic
TGCTTGCAGGTTATGTGTGG+AGG	0.634525	3.4:-61563188	MS.gene38527:CDS
CAGTAACGGTTCTAACGGAG+TGG	0.637528	3.4:-61562861	MS.gene38527:CDS
GTGGTTTCACAATTGGAATG+GGG	0.639088	3.4:-61563524	MS.gene38527:CDS
AGACTAGTGAGACATGCTGA+TGG	0.640547	3.4:-61563747	MS.gene38527:CDS
GAAAATACACGTATCGATGG+TGG	0.648323	3.4:+61562756	None:intergenic
CTCTTTGGAGACGAATCAAG+AGG	0.650080	3.4:-61562917	MS.gene38527:CDS
CATGATCCATCAATGGATGG+AGG	0.654646	3.4:-61562709	MS.gene38527:CDS
AGAGTCAATGCCAATACCAA+CGG	0.657356	3.4:-61563717	MS.gene38527:CDS
ACATAGGCATAGCAGCAACA+GGG	0.673752	3.4:+61562665	None:intergenic
AGCCATCAGTGAAACCTGTG+TGG	0.685980	3.4:-61563422	MS.gene38527:CDS
ATGGTGGCAAGCATCTTGCA+TGG	0.692293	3.4:+61562772	None:intergenic
GCCATCAGTGAAACCTGTGT+GGG	0.717270	3.4:-61563421	MS.gene38527:CDS
AGCATGGAATTGGAAGCTAG+TGG	0.746349	3.4:-61562886	MS.gene38527:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	CAATTTTTTAAAAATTTATG+TGG	-	chr3.4:61564256-61564275	MS.gene38527:intron	10.0%
!!!	GAAAAAAAATAGTTTTATTT+AGG	+	chr3.4:61561455-61561474	None:intergenic	10.0%
!!	AAAAGACTAATAATTACTAT+CGG	+	chr3.4:61561250-61561269	None:intergenic	15.0%
!!	AATACTATAAAACAGAAATT+AGG	+	chr3.4:61563692-61563711	None:intergenic	15.0%
!!	ATACTATAAAACAGAAATTA+GGG	+	chr3.4:61563691-61563710	None:intergenic	15.0%
!!	TAAATAAAGGTCATATATTA+TGG	-	chr3.4:61561641-61561660	MS.gene38527:intron	15.0%
!!	TTAATTATATATATCCTCTT+TGG	-	chr3.4:61563741-61563760	MS.gene38527:CDS	15.0%
!!!	AAACAATTTTTTTTCACATA+TGG	-	chr3.4:61562174-61562193	MS.gene38527:intron	15.0%
!!!	ACAATATTGTTTTAAAGTAA+AGG	-	chr3.4:61562761-61562780	MS.gene38527:CDS	15.0%
!!!	CAATATTGTTTTAAAGTAAA+GGG	-	chr3.4:61562762-61562781	MS.gene38527:CDS	15.0%
!!!	CTTAAATGAAGTTTATTTTA+TGG	+	chr3.4:61561275-61561294	None:intergenic	15.0%
!!!	TAGAGAGATTTTTTTTTTAA+TGG	-	chr3.4:61564103-61564122	MS.gene38527:intron	15.0%
!!!	TTCATTTAAGTTCTAAAATA+AGG	-	chr3.4:61561285-61561304	MS.gene38527:intron	15.0%
!!!	TTTTATCATGAAATGTTATA+AGG	-	chr3.4:61563785-61563804	MS.gene38527:intron	15.0%
!!!	TTTTTAAAAATTTATGTGGA+CGG	-	chr3.4:61564260-61564279	MS.gene38527:intron	15.0%
!!	ATTATGATACTTTACCTAAA+GGG	-	chr3.4:61562961-61562980	MS.gene38527:CDS	20.0%
!!	ATTCATCTACTATCAAAAAA+AGG	+	chr3.4:61561622-61561641	None:intergenic	20.0%
!!	CAATGTTGTTAAAAATTTGT+TGG	+	chr3.4:61564352-61564371	None:intergenic	20.0%
!!	CATTATTTCATTAAACAACA+AGG	-	chr3.4:61563832-61563851	MS.gene38527:intron	20.0%
!!	GATAGTAGATGAATAAATAA+AGG	-	chr3.4:61561628-61561647	MS.gene38527:intron	20.0%
!!	GTGAAAAAAAATTGTTTGTT+AGG	+	chr3.4:61562171-61562190	None:intergenic	20.0%
!!	TGAATGAAAATGAAAACATA+AGG	-	chr3.4:61562817-61562836	MS.gene38527:CDS	20.0%
!!	TGTAGTTTATAATGTGTAAA+AGG	-	chr3.4:61564303-61564322	MS.gene38527:intron	20.0%
!!	TTTAATGAAATAATGTATCG+AGG	+	chr3.4:61563827-61563846	None:intergenic	20.0%
!!	TTTCTTTGTTGAAATTAAAG+TGG	-	chr3.4:61563428-61563447	MS.gene38527:CDS	20.0%
!!!	AAAAAAATCTCTCTAAGAAT+TGG	+	chr3.4:61564098-61564117	None:intergenic	20.0%
!!!	ACCTCTAAAGTAAAAAATAT+AGG	+	chr3.4:61561790-61561809	None:intergenic	20.0%
!!!	AGTATTATTTTGATTCACAA+AGG	+	chr3.4:61562730-61562749	None:intergenic	20.0%
!!!	ATAGACATAATGAAAGTATT+AGG	-	chr3.4:61563858-61563877	MS.gene38527:intron	20.0%
!!!	CAATTTTTTTTTTCAGAAGA+TGG	+	chr3.4:61562250-61562269	None:intergenic	20.0%
!!!	CATCTTCTGAAAAAAAAAAT+TGG	-	chr3.4:61562248-61562267	MS.gene38527:intron	20.0%
!!!	GTATTATTTTGATTCACAAA+GGG	+	chr3.4:61562729-61562748	None:intergenic	20.0%
!!!	GTTAAAAATTTGTTGGTTTA+AGG	+	chr3.4:61564345-61564364	None:intergenic	20.0%
!!!	TAGACATAATGAAAGTATTA+GGG	-	chr3.4:61563859-61563878	MS.gene38527:intron	20.0%
!!!	TCCTATATTTTTTACTTTAG+AGG	-	chr3.4:61561786-61561805	MS.gene38527:intron	20.0%
!!!	TTGAAAATGATTTTTGGTTA+GGG	+	chr3.4:61564194-61564213	None:intergenic	20.0%
!!!	TTTCAGGAAAATTTAGATTT+TGG	-	chr3.4:61563218-61563237	MS.gene38527:CDS	20.0%
!	ATAGAGAAAGAATACAAGAA+GGG	+	chr3.4:61561039-61561058	None:intergenic	25.0%
!	ATTGCTTGTATTATGAATGA+TGG	+	chr3.4:61564160-61564179	None:intergenic	25.0%
!	GATTATGATACTTTACCTAA+AGG	-	chr3.4:61562960-61562979	MS.gene38527:CDS	25.0%
!	TAAAAATATTGCTTTCACTG+CGG	-	chr3.4:61563082-61563101	MS.gene38527:CDS	25.0%
!	TAAAACAGAAATTAGGGATA+AGG	+	chr3.4:61563685-61563704	None:intergenic	25.0%
!	TACACACAAAAATTGTCAAT+CGG	+	chr3.4:61563558-61563577	None:intergenic	25.0%
!	TCATGATCATCAAGTTTATT+AGG	+	chr3.4:61563320-61563339	None:intergenic	25.0%
!	TTGCTTGTATTATGAATGAT+GGG	+	chr3.4:61564159-61564178	None:intergenic	25.0%
!!	AATTAAAGTGGATTTGGATA+TGG	-	chr3.4:61563440-61563459	MS.gene38527:CDS	25.0%
!!	ATCTTCCTTTTCAATCTTTA+GGG	+	chr3.4:61560987-61561006	None:intergenic	25.0%
!!	GTTTTTGCAATTTTATAGCT+GGG	-	chr3.4:61564004-61564023	MS.gene38527:intron	25.0%
!!	TAAAACAACTTTTGCATCTA+TGG	+	chr3.4:61563003-61563022	None:intergenic	25.0%
!!	TAATTTCTCCTTGTTCTTTT+CGG	+	chr3.4:61563960-61563979	None:intergenic	25.0%
!!	TGTGAATATCTTGTTTGTTT+GGG	+	chr3.4:61563894-61563913	None:intergenic	25.0%
!!	TGTTGAAATTAAAGTGGATT+TGG	-	chr3.4:61563434-61563453	MS.gene38527:CDS	25.0%
!!	TTGTGAATATCTTGTTTGTT+TGG	+	chr3.4:61563895-61563914	None:intergenic	25.0%
!!	TTTTACTATTATCCTTTAGC+AGG	-	chr3.4:61561196-61561215	MS.gene38527:intron	25.0%
!!!	AAAATTTTAGGAACTTCTGA+AGG	+	chr3.4:61564839-61564858	None:intergenic	25.0%
!!!	AACTTTTTTTCTTCTCTCTT+GGG	-	chr3.4:61561561-61561580	MS.gene38527:intron	25.0%
!!!	ACAATGCTAAATGTTTTAGT+TGG	-	chr3.4:61562918-61562937	MS.gene38527:CDS	25.0%
!!!	AGACATAATGAAAGTATTAG+GGG	-	chr3.4:61563860-61563879	MS.gene38527:intron	25.0%
!!!	CTTGAAAATGATTTTTGGTT+AGG	+	chr3.4:61564195-61564214	None:intergenic	25.0%
	AAAACTAGTATACCTGCTAA+AGG	+	chr3.4:61561211-61561230	None:intergenic	30.0%
	AAGGAGAAATTAGATTGATG+GGG	-	chr3.4:61563968-61563987	MS.gene38527:intron	30.0%
	AATAAGTTCTTTGTGAAACC+TGG	-	chr3.4:61564889-61564908	MS.gene38527:CDS	30.0%
	AATTGTTTGAAGATCATCCA+AGG	+	chr3.4:61564690-61564709	None:intergenic	30.0%
	ACAAGGAGAAATTAGATTGA+TGG	-	chr3.4:61563966-61563985	MS.gene38527:intron	30.0%
	ACACACAAAAATTGTCAATC+GGG	+	chr3.4:61563557-61563576	None:intergenic	30.0%
	ACTTAAAATTCACTCGTGAT+TGG	-	chr3.4:61563522-61563541	MS.gene38527:CDS	30.0%
	AGTAAGATTCAAAGACTCAA+CGG	-	chr3.4:61563627-61563646	MS.gene38527:CDS	30.0%
	AGTCTCTTTAATCAGCTTAA+CGG	+	chr3.4:61563063-61563082	None:intergenic	30.0%
	AGTTAGGGCAATGTTATTAT+TGG	+	chr3.4:61561083-61561102	None:intergenic	30.0%
	AGTTGTACAATAATCCAAAG+AGG	+	chr3.4:61563758-61563777	None:intergenic	30.0%
	ATTGTGTGAGTATTTGGTAA+AGG	-	chr3.4:61564402-61564421	MS.gene38527:intron	30.0%
	CAAATACTCACACAATTCAT+AGG	+	chr3.4:61564398-61564417	None:intergenic	30.0%
	CAAGGAGAAATTAGATTGAT+GGG	-	chr3.4:61563967-61563986	MS.gene38527:intron	30.0%
	CACAAACTCAATAAACTCTT+TGG	-	chr3.4:61563112-61563131	MS.gene38527:CDS	30.0%
	CACAGAAATTGCAAAACATT+TGG	-	chr3.4:61562428-61562447	MS.gene38527:intron	30.0%
	CTATCAACACTTGACAATAA+AGG	-	chr3.4:61561055-61561074	MS.gene38527:CDS	30.0%
	CTATGAATTGTGTGAGTATT+TGG	-	chr3.4:61564396-61564415	MS.gene38527:intron	30.0%
	CTTCTTTGAATACATGACAA+GGG	+	chr3.4:61561159-61561178	None:intergenic	30.0%
	GAGGGTTGAGAATATAAATA+TGG	+	chr3.4:61564517-61564536	None:intergenic	30.0%
	GATAGAGAAAGAATACAAGA+AGG	+	chr3.4:61561040-61561059	None:intergenic	30.0%
!	AAAGGGTAACTTTTATCCTT+GGG	+	chr3.4:61562471-61562490	None:intergenic	30.0%
!	AGTCCAGATTGCAAAATTTT+AGG	+	chr3.4:61564851-61564870	None:intergenic	30.0%
!	CAGGAAAATTTAGATTTTGG+AGG	-	chr3.4:61563221-61563240	MS.gene38527:CDS	30.0%
!	CATCTTCCTTTTCAATCTTT+AGG	+	chr3.4:61560988-61561007	None:intergenic	30.0%
!	CCTCTCTTGAAAATGATTTT+TGG	+	chr3.4:61564200-61564219	None:intergenic	30.0%
!	GGTTTTTGCAATTTTATAGC+TGG	-	chr3.4:61564003-61564022	MS.gene38527:intron	30.0%
!	GTTCCTAAAATTTTGCAATC+TGG	-	chr3.4:61564845-61564864	MS.gene38527:intron	30.0%
!	TTTTGTGTGTAAAATCCGAT+TGG	-	chr3.4:61563567-61563586	MS.gene38527:CDS	30.0%
!!	AAATATGGAAGCATCTAGTA+GGG	+	chr3.4:61564502-61564521	None:intergenic	30.0%
!!	ACTTTACCTAAAGGGTATTA+TGG	-	chr3.4:61562969-61562988	MS.gene38527:CDS	30.0%
!!	CCAAAAATCATTTTCAAGAG+AGG	-	chr3.4:61564197-61564216	MS.gene38527:intron	30.0%
!!	TAAATATGGAAGCATCTAGT+AGG	+	chr3.4:61564503-61564522	None:intergenic	30.0%
!!	TTCTGAAGTTACAATCCAAT+CGG	+	chr3.4:61563585-61563604	None:intergenic	30.0%
!!!	GAACTTTTTTTCTTCTCTCT+TGG	-	chr3.4:61561560-61561579	MS.gene38527:intron	30.0%
!!!	TTGTTGTTTGCTTGTTTTGT+AGG	-	chr3.4:61562273-61562292	MS.gene38527:intron	30.0%
	AAACCAAAATTCTTTGGTCC+TGG	-	chr3.4:61562093-61562112	MS.gene38527:intron	35.0%
	AAATTCAGTACCGTTGGTAT+TGG	+	chr3.4:61562340-61562359	None:intergenic	35.0%
	AAGAGAGAAGAGAGAGAAAA+TGG	-	chr3.4:61562022-61562041	MS.gene38527:intron	35.0%
	ACTTATCAGCATAGTTCACA+AGG	+	chr3.4:61564637-61564656	None:intergenic	35.0%
	AGAAAGAATACAAGAAGGGA+TGG	+	chr3.4:61561035-61561054	None:intergenic	35.0%
	AGCAACCCTAAAGATTGAAA+AGG	-	chr3.4:61560979-61560998	MS.gene38527:CDS	35.0%
	ATCGGTATACATGAACAGTT+AGG	+	chr3.4:61561099-61561118	None:intergenic	35.0%
	ATGGTGACAAAGTTTCTTTG+TGG	-	chr3.4:61562498-61562517	MS.gene38527:intron	35.0%
	CAAGGATAAAAGTTACCCTT+TGG	-	chr3.4:61562470-61562489	MS.gene38527:intron	35.0%
	CAGGTAATGAAGAGAAGAAA+GGG	+	chr3.4:61562397-61562416	None:intergenic	35.0%
	CTCGTATGAGTGTTATATAC+AGG	+	chr3.4:61561511-61561530	None:intergenic	35.0%
	GAATTGCCATAATACCCTTT+AGG	+	chr3.4:61562978-61562997	None:intergenic	35.0%
	GCTTCTTTGAATACATGACA+AGG	+	chr3.4:61561160-61561179	None:intergenic	35.0%
	GGGATAAGGTACAAATGAAA+TGG	+	chr3.4:61563671-61563690	None:intergenic	35.0%
	GGGATGAAATTGAAGCTTTA+AGG	-	chr3.4:61563405-61563424	MS.gene38527:CDS	35.0%
	GTGACCAAACCAAAATTCTT+TGG	-	chr3.4:61562087-61562106	MS.gene38527:intron	35.0%
	TAACCTTGCATACTCCTTAT+AGG	+	chr3.4:61564565-61564584	None:intergenic	35.0%
	TCAATTTCATCCCTGAACAT+AGG	+	chr3.4:61563398-61563417	None:intergenic	35.0%
	TCGGTATACATGAACAGTTA+GGG	+	chr3.4:61561098-61561117	None:intergenic	35.0%
	TCTTGAAACTGAATCATCTC+AGG	+	chr3.4:61564940-61564959	None:intergenic	35.0%
	TGAGAAAATACACGTATCGA+TGG	+	chr3.4:61563294-61563313	None:intergenic	35.0%
	TGAGTGTTATATACAGGTGT+CGG	+	chr3.4:61561505-61561524	None:intergenic	35.0%
	TGATGATCATGATCCATCAA+TGG	-	chr3.4:61563328-61563347	MS.gene38527:CDS	35.0%
	TGCATACTCCTTATAGGAAA+AGG	+	chr3.4:61564559-61564578	None:intergenic	35.0%
	TGGGAAAGAGAAAAACAAGT+TGG	-	chr3.4:61562642-61562661	MS.gene38527:CDS	35.0%
	TTACTTCAACACTTCTACAC+CGG	-	chr3.4:61561476-61561495	MS.gene38527:intron	35.0%
	TTGTCACCATTAACACCAAA+GGG	+	chr3.4:61562488-61562507	None:intergenic	35.0%
	TTGTGAACTATGCTGATAAG+TGG	-	chr3.4:61564636-61564655	MS.gene38527:intron	35.0%
	TTTCTGTGTTAGTATTGTCC+AGG	+	chr3.4:61562416-61562435	None:intergenic	35.0%
	TTTGGTAAAGGTTGCACAAT+TGG	-	chr3.4:61564414-61564433	MS.gene38527:intron	35.0%
	TTTGTCACCATTAACACCAA+AGG	+	chr3.4:61562489-61562508	None:intergenic	35.0%
!	AATACCAACGGTACTGAATT+TGG	-	chr3.4:61562339-61562358	MS.gene38527:intron	35.0%
!	ATACCAACGGTACTGAATTT+GGG	-	chr3.4:61562340-61562359	MS.gene38527:intron	35.0%
!	ATGTCACATGCTATTTGTGA+CGG	-	chr3.4:61562543-61562562	MS.gene38527:intron	35.0%
!	CAAAGGGTAACTTTTATCCT+TGG	+	chr3.4:61562472-61562491	None:intergenic	35.0%
!	CATGAATTGCTGATGATGAT+CGG	+	chr3.4:61561117-61561136	None:intergenic	35.0%
!	CTTTTTATCACTATCTGACC+AGG	+	chr3.4:61564910-61564929	None:intergenic	35.0%
!	GTAACAGATTTTCTGTCCAT+GGG	+	chr3.4:61562699-61562718	None:intergenic	35.0%
!	GTCATTGCTGCTAAGTTTTA+AGG	+	chr3.4:61562895-61562914	None:intergenic	35.0%
!	TAAAGTGGATTTGGATATGG+TGG	-	chr3.4:61563443-61563462	MS.gene38527:CDS	35.0%
!	TAACTTTTATCCTTGGGAGA+AGG	+	chr3.4:61562465-61562484	None:intergenic	35.0%
!	TGTAACAGATTTTCTGTCCA+TGG	+	chr3.4:61562700-61562719	None:intergenic	35.0%
!	TTGACTTCCTTCTAAAGAGT+CGG	-	chr3.4:61564369-61564388	MS.gene38527:intron	35.0%
!	TTTAGAGTCTGAAATCTGTC+TGG	+	chr3.4:61564801-61564820	None:intergenic	35.0%
!	TTTCCTATAAGGAGTATGCA+AGG	-	chr3.4:61564559-61564578	MS.gene38527:intron	35.0%
!!	AAGTTACCCTTTGGTGTTAA+TGG	-	chr3.4:61562479-61562498	MS.gene38527:intron	35.0%
!!	TGAATTTGGGGTTCCATTTT+TGG	-	chr3.4:61562353-61562372	MS.gene38527:intron	35.0%
!!!	AGGACCAAAGAATTTTGGTT+TGG	+	chr3.4:61562094-61562113	None:intergenic	35.0%
	AAAAGGCTAGAGGTTAAGGT+AGG	+	chr3.4:61564542-61564561	None:intergenic	40.0%
	AAGAAGAGCTGGCTAGATAA+TGG	-	chr3.4:61565082-61565101	MS.gene38527:CDS	40.0%
	AATCCAACCAACAACAAGAG+AGG	-	chr3.4:61563914-61563933	MS.gene38527:intron	40.0%
	ACACACGAGAAAATCTTGTG+TGG	-	chr3.4:61561943-61561962	MS.gene38527:intron	40.0%
	AGAGTCAATGCCAATACCAA+CGG	-	chr3.4:61562327-61562346	MS.gene38527:intron	40.0%
	AGCTTAACGGTTTCATAGAG+TGG	+	chr3.4:61563050-61563069	None:intergenic	40.0%
	ATGAAGCATAGACTCCAACA+CGG	+	chr3.4:61561847-61561866	None:intergenic	40.0%
	CCAGGTAATGAAGAGAAGAA+AGG	+	chr3.4:61562398-61562417	None:intergenic	40.0%
	CCTTTCTTCTCTTCATTACC+TGG	-	chr3.4:61562395-61562414	MS.gene38527:intron	40.0%
	CTAACGGAGTGGAAACATTT+GGG	-	chr3.4:61563194-61563213	MS.gene38527:CDS	40.0%
	CTGATAAGTGGCAGAATCTA+AGG	-	chr3.4:61564648-61564667	MS.gene38527:intron	40.0%
	CTTTGTGGTGGTTTCACAAT+TGG	-	chr3.4:61562513-61562532	MS.gene38527:intron	40.0%
	GACCAAATCCGAAAAGAACA+AGG	-	chr3.4:61563949-61563968	MS.gene38527:intron	40.0%
	GAGAAATTAGATTGATGGGG+TGG	-	chr3.4:61563971-61563990	MS.gene38527:intron	40.0%
	GATCATGATCCATCAATGGA+TGG	-	chr3.4:61563332-61563351	MS.gene38527:CDS	40.0%
	GCATAAAGAGCGATTGTCTT+CGG	+	chr3.4:61563502-61563521	None:intergenic	40.0%
	GGTGGTTTCACAATTGGAAT+GGG	-	chr3.4:61562519-61562538	MS.gene38527:intron	40.0%
	GTGACAAAGTTTCTTTGTGG+TGG	-	chr3.4:61562501-61562520	MS.gene38527:intron	40.0%
	GTGGTTTCACAATTGGAATG+GGG	-	chr3.4:61562520-61562539	MS.gene38527:intron	40.0%
	TACATGACAAGGGTTGAAAC+TGG	+	chr3.4:61561149-61561168	None:intergenic	40.0%
	TAGGAAAAGGCTAGAGGTTA+AGG	+	chr3.4:61564546-61564565	None:intergenic	40.0%
	TCCTTATAGGAAAAGGCTAG+AGG	+	chr3.4:61564552-61564571	None:intergenic	40.0%
	TCTAACGGAGTGGAAACATT+TGG	-	chr3.4:61563193-61563212	MS.gene38527:CDS	40.0%
	TCTTGATATTCCTTCTCCCA+AGG	-	chr3.4:61562452-61562471	MS.gene38527:intron	40.0%
	TGGTATCAGACGAAACTTGA+TGG	-	chr3.4:61562938-61562957	MS.gene38527:CDS	40.0%
	TGGTGGTTTCACAATTGGAA+TGG	-	chr3.4:61562518-61562537	MS.gene38527:intron	40.0%
	TGTTGGAGTCTATGCTTCAT+AGG	-	chr3.4:61561847-61561866	MS.gene38527:intron	40.0%
	TTAGATTGATGGGGTGGTTT+CGG	-	chr3.4:61563977-61563996	MS.gene38527:intron	40.0%
	TTAGCAGCTGCTTCCAAAAA+TGG	+	chr3.4:61562369-61562388	None:intergenic	40.0%
!	CTCCTTGTTCTTTTCGGATT+TGG	+	chr3.4:61563954-61563973	None:intergenic	40.0%
!	GAAAATACACGTATCGATGG+TGG	+	chr3.4:61563291-61563310	None:intergenic	40.0%
!	GTTGATGAGAAACACTTGTG+TGG	+	chr3.4:61562120-61562139	None:intergenic	40.0%
!	TACCAACGGTACTGAATTTG+GGG	-	chr3.4:61562341-61562360	MS.gene38527:intron	40.0%
!	TGACTTCCTTCTAAAGAGTC+GGG	-	chr3.4:61564370-61564389	MS.gene38527:intron	40.0%
!	TGATAAGCCTCTCTTGTTGT+TGG	+	chr3.4:61563924-61563943	None:intergenic	40.0%
!	TGCAACACGTCTAGTTTAAG+AGG	+	chr3.4:61561359-61561378	None:intergenic	40.0%
!	TGTTTTTCTCTTTCCCACAC+AGG	+	chr3.4:61562639-61562658	None:intergenic	40.0%
!!	ACCTCTAGCCTTTTCCTATA+AGG	-	chr3.4:61564548-61564567	MS.gene38527:intron	40.0%
!!	CAAGAAGGGATGGATTTTGA+TGG	+	chr3.4:61561025-61561044	None:intergenic	40.0%
!!	CATGCTATTTGTGACGGATT+TGG	-	chr3.4:61562549-61562568	MS.gene38527:intron	40.0%
!!	GTGGAAACATTTGGGTTTTC+AGG	-	chr3.4:61563202-61563221	MS.gene38527:CDS	40.0%
!!	TGATGGGTGACTTCTTTTGT+CGG	+	chr3.4:61564143-61564162	None:intergenic	40.0%
!!!	ACAACTTTTGAAGTGCTTGC+AGG	-	chr3.4:61562843-61562862	MS.gene38527:CDS	40.0%
	AAAGAGAACTTGTGTGGACC+AGG	+	chr3.4:61561970-61561989	None:intergenic	45.0%
	AACACTCATACGAGTCGTGT+CGG	-	chr3.4:61561517-61561536	MS.gene38527:intron	45.0%
	AACATAGGCATAGCAGCAAC+AGG	+	chr3.4:61563383-61563402	None:intergenic	45.0%
	AACCCCAAATTCAGTACCGT+TGG	+	chr3.4:61562346-61562365	None:intergenic	45.0%
	AAGTGCTTGCAGGTTATGTG+TGG	-	chr3.4:61562853-61562872	MS.gene38527:CDS	45.0%
	ACATAGGCATAGCAGCAACA+GGG	+	chr3.4:61563382-61563401	None:intergenic	45.0%
	ACCATTTCCACAATGTCCCA+TGG	-	chr3.4:61562680-61562699	MS.gene38527:CDS	45.0%
	ACGTGTCGTATGAGTGTCAT+AGG	+	chr3.4:61561411-61561430	None:intergenic	45.0%
	AGACTAGTGAGACATGCTGA+TGG	-	chr3.4:61562297-61562316	MS.gene38527:intron	45.0%
	AGCATGGAATTGGAAGCTAG+TGG	-	chr3.4:61563158-61563177	MS.gene38527:CDS	45.0%
	AGTCAGAAGACAAGAAGAGC+TGG	-	chr3.4:61565071-61565090	MS.gene38527:CDS	45.0%
	CAATTGCGCAATCATCTCCT+GGG	+	chr3.4:61564455-61564474	None:intergenic	45.0%
	CATCAATGGATGGAGGAGTT+AGG	-	chr3.4:61563342-61563361	MS.gene38527:CDS	45.0%
	CATGATCCATCAATGGATGG+AGG	-	chr3.4:61563335-61563354	MS.gene38527:CDS	45.0%
	CTAACTCCTCCATCCATTGA+TGG	+	chr3.4:61563344-61563363	None:intergenic	45.0%
	GAGAAAATCTTGTGTGGTCC+TGG	-	chr3.4:61561949-61561968	MS.gene38527:intron	45.0%
	GAGAGAAGAGAAGAGAGAGA+AGG	-	chr3.4:61562049-61562068	MS.gene38527:intron	45.0%
	GCAGTTGTTGAACTTGCAAG+TGG	-	chr3.4:61562591-61562610	MS.gene38527:intron	45.0%
	GGATGGTTTGAGCATGGAAT+TGG	-	chr3.4:61563148-61563167	MS.gene38527:CDS	45.0%
	GTGACCAAAGAGAACTTGTG+TGG	+	chr3.4:61561976-61561995	None:intergenic	45.0%
	TAGAGGTTAAGGTAGGAGGA+GGG	+	chr3.4:61564535-61564554	None:intergenic	45.0%
	TAGGTACCCGACTCTTTAGA+AGG	+	chr3.4:61564379-61564398	None:intergenic	45.0%
	TCAAGAGGATGGTTTGAGCA+TGG	-	chr3.4:61563142-61563161	MS.gene38527:CDS	45.0%
	TCCATGGGACATTGTGGAAA+TGG	+	chr3.4:61562684-61562703	None:intergenic	45.0%
	TGCCTGACACAATACTGACA+CGG	+	chr3.4:61561823-61561842	None:intergenic	45.0%
	TGCTGCTATGCCTATGTTCA+GGG	-	chr3.4:61563385-61563404	MS.gene38527:CDS	45.0%
	TGGTCCACACAAGTTCTCTT+TGG	-	chr3.4:61561969-61561988	MS.gene38527:intron	45.0%
	TTGCTGCTATGCCTATGTTC+AGG	-	chr3.4:61563384-61563403	MS.gene38527:CDS	45.0%
	TTGGAGACGAATCAAGAGGA+TGG	-	chr3.4:61563131-61563150	MS.gene38527:CDS	45.0%
	TTTCTGTCCATGGGACATTG+TGG	+	chr3.4:61562690-61562709	None:intergenic	45.0%
!	CTCTTTGGAGACGAATCAAG+AGG	-	chr3.4:61563127-61563146	MS.gene38527:CDS	45.0%
!	GGACCAGGACCAAAGAATTT+TGG	+	chr3.4:61562099-61562118	None:intergenic	45.0%
!	TGGTGCAGTAACGGTTCTAA+CGG	-	chr3.4:61563178-61563197	MS.gene38527:CDS	45.0%
!!	AAGCCTCTCTTGTTGTTGGT+TGG	+	chr3.4:61563920-61563939	None:intergenic	45.0%
!!	ATTCAAAGAAGCGCTGTCGA+AGG	-	chr3.4:61561168-61561187	MS.gene38527:CDS	45.0%
!!	GCAGTTGGTGAATAGAACCT+TGG	-	chr3.4:61564670-61564689	MS.gene38527:intron	45.0%
!!	TATATACAGGTGTCGGACAC+CGG	+	chr3.4:61561498-61561517	None:intergenic	45.0%
!!	ATAAATTAATATTTATATAG+CGG	-	chr3.4:61561755-61561774	MS.gene38527:intron	5.0%
	AGCAAGAAGCTCTCAGAAGC+TGG	-	chr3.4:61564723-61564742	MS.gene38527:intron	50.0%
	AGCCATCAGTGAAACCTGTG+TGG	-	chr3.4:61562622-61562641	MS.gene38527:intron	50.0%
	AGGCTAGAGGTTAAGGTAGG+AGG	+	chr3.4:61564539-61564558	None:intergenic	50.0%
	ATGGTGGCAAGCATCTTGCA+TGG	+	chr3.4:61563275-61563294	None:intergenic	50.0%
	ATTGTGTCAGGCATCCGTGT+TGG	-	chr3.4:61561830-61561849	MS.gene38527:intron	50.0%
	CTAGAGGTTAAGGTAGGAGG+AGG	+	chr3.4:61564536-61564555	None:intergenic	50.0%
	GAGAAACACTTGTGTGGACC+AGG	+	chr3.4:61562114-61562133	None:intergenic	50.0%
	GCAATTGCGCAATCATCTCC+TGG	+	chr3.4:61564456-61564475	None:intergenic	50.0%
	GCCATCAGTGAAACCTGTGT+GGG	-	chr3.4:61562623-61562642	MS.gene38527:intron	50.0%
	GCTAGTGGAAGCAAGACATC+AGG	-	chr3.4:61564604-61564623	MS.gene38527:intron	50.0%
	GGAAGCTAGTGGTGCAGTAA+CGG	-	chr3.4:61563169-61563188	MS.gene38527:CDS	50.0%
	GGAGCTTCTAAATTCGTCCC+AGG	-	chr3.4:61564435-61564454	MS.gene38527:intron	50.0%
	GTCCGTGTCAGTATTGTGTC+AGG	-	chr3.4:61561818-61561837	MS.gene38527:intron	50.0%
	GTGGATTTGGATATGGTGGC+TGG	-	chr3.4:61563447-61563466	MS.gene38527:CDS	50.0%
	TCCCACACAGGTTTCACTGA+TGG	+	chr3.4:61562627-61562646	None:intergenic	50.0%
	TGCTTGCAGGTTATGTGTGG+AGG	-	chr3.4:61562856-61562875	MS.gene38527:CDS	50.0%
!	AAGAGGTGTGTGCGCTTCAT+AGG	+	chr3.4:61561342-61561361	None:intergenic	50.0%
!	CAGTAACGGTTCTAACGGAG+TGG	-	chr3.4:61563183-61563202	MS.gene38527:CDS	50.0%
!	GTCTTGCTTCCACTAGCTCT+TGG	+	chr3.4:61564601-61564620	None:intergenic	50.0%
!	GTGGCAGAATCTAAGGCAGT+TGG	-	chr3.4:61564655-61564674	MS.gene38527:intron	50.0%
!	TTGCTTCCACTAGCTCTTGG+AGG	+	chr3.4:61564598-61564617	None:intergenic	50.0%
!!	AGAAGTCTGCAGAAGCATCG+AGG	-	chr3.4:61564751-61564770	MS.gene38527:intron	50.0%
	GATATGGTGGCTGGTGCAGT+GGG	-	chr3.4:61563456-61563475	MS.gene38527:CDS	55.0%
	GCAAGCATCTTGCATGGTGC+AGG	+	chr3.4:61563269-61563288	None:intergenic	55.0%
	TGACAGCCTCCAAGAGCTAG+TGG	-	chr3.4:61564589-61564608	MS.gene38527:intron	55.0%
	TTGCAGTGTCCAACACGCGT+CGG	-	chr3.4:61561374-61561393	MS.gene38527:intron	55.0%
!!	CTGCAGAAGCATCGAGGAAG+TGG	-	chr3.4:61564757-61564776	MS.gene38527:intron	55.0%
	GGATATGGTGGCTGGTGCAG+TGG	-	chr3.4:61563455-61563474	MS.gene38527:CDS	60.0%
	TGTCGAACACCGACGCGTGT+TGG	+	chr3.4:61561386-61561405	None:intergenic	60.0%
	TTGATGGGGTGGTTTCGGCG+AGG	-	chr3.4:61563982-61564001	MS.gene38527:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	61560965	61565101	61560965	ID=MS.gene38527
chr3.4	mRNA	61560965	61565101	61560965	ID=MS.gene38527.t1;Parent=MS.gene38527
chr3.4	exon	61564849	61565101	61564849	ID=MS.gene38527.t1.exon1;Parent=MS.gene38527.t1
chr3.4	CDS	61564849	61565101	61564849	ID=cds.MS.gene38527.t1;Parent=MS.gene38527.t1
chr3.4	exon	61562640	61563771	61562640	ID=MS.gene38527.t1.exon2;Parent=MS.gene38527.t1
chr3.4	CDS	61562640	61563771	61562640	ID=cds.MS.gene38527.t1;Parent=MS.gene38527.t1
chr3.4	exon	61560965	61561190	61560965	ID=MS.gene38527.t1.exon3;Parent=MS.gene38527.t1
chr3.4	CDS	61560965	61561190	61560965	ID=cds.MS.gene38527.t1;Parent=MS.gene38527.t1

Gene Sequence

>MS.gene38527

ATGGAATATGAGAAAGCAACCCTAAAGATTGAAAAGGAAGATGTTTTGCTTGTAAAACCATCAAAATCCATCCCTTCTTGTATTCTTTCTCTATCAACACTTGACAATAAAGGTGCCAATAATAACATTGCCCTAACTGTTCATGTATACCGATCATCATCAGCAATTCATGATTATCATTCCAGTTTCAACCCTTGTCATGTATTCAAAGAAGCGCTGTCGAAGGCTTTGTTTTACTATTATCCTTTAGCAGGTATACTAGTTTTTTTTCTTTAAGAACAACCGATAGTAATTATTAGTCTTTTTACCATAAAATAAACTTCATTTAAGTTCTAAAATAAGGAGAGTACAATCAATTTGTCTATGAGATGTAACCTATGAAGCGCACACACCTCTTAAACTAGACGTGTTGCAGTGTCCAACACGCGTCGGTGTTCGACAGTCCTATGACACTCATACGACACGTGTCGAACAACTAATATTGTCTCCTAAATAAAACTATTTTTTTTCTTTACTTCAACACTTCTACACCGGTGTCCGACACCTGTATATAACACTCATACGAGTCGTGTCGGACAACTCAGACAAATATCTAGAACTTTTTTTCTTCTCTCTTGGGAGTTATAGAAATTAAAGTTAATTACAATATTTTTACCTTTTTTTGATAGTAGATGAATAAATAAAGGTCATATATTATGGTATCTTGTTTTAGAACTTTTTTCAAAGAAATAAATTGCTTAAGTTAGATGCTTATGTATGTGTTTTGATTTTCATTTTAGAATGTCTATAAATAAATTAATATTTATATAGCGGTGTCATTGTCCTATATTTTTTACTTTAGAGGTGTCGATTTGTCCGTGTCAGTATTGTGTCAGGCATCCGTGTTGGAGTCTATGCTTCATAGGATGTAACAAAAGAAAAGTTTGACTATGAGTTGAAACATTTTTCATCTATATTTTTAAAGTTAACACGCTATCAACACACGAGAAAATCTTGTGTGGTCCTGGTCCACACAAGTTCTCTTTGGTCACACAAAGAGAAAAAAGAGAAGAGAGAGAAGAGAGAAGAGAGAGAAAATGGAGAAGAGAGAAGAGAAGAGAGAGAAGGAAAAAACTTGTGTGTGTGACCAAACCAAAATTCTTTGGTCCTGGTCCACACAAGTGTTTCTCATCAACACACATAGAAATTTCAGTAGCGAAAAGTCCTAACAAACAATTTTTTTTCACATATGGCTAATAGTCAAGAATAATGAAAGATTCACAAACATTAATTGCATTTGCATCCATCTTCTGAAAAAAAAAATTGGTTTTGTTGTTTGCTTGTTTTGTAGGTAGACTAGTGAGACATGCTGATGGAAAATTTAGAGTCAATGCCAATACCAACGGTACTGAATTTGGGGTTCCATTTTTGGAAGCAGCTGCTAATTGCACCCTTTCTTCTCTTCATTACCTGGACAATACTAACACAGAAATTGCAAAACATTTGGTTCTTGATATTCCTTCTCCCAAGGATAAAAGTTACCCTTTGGTGTTAATGGTGACAAAGTTTCTTTGTGGTGGTTTCACAATTGGAATGGGGATGTCACATGCTATTTGTGACGGATTTGGTGCATCGCAATTCTTCAAAGCAGTTGTTGAACTTGCAAGTGGAAGAACTGAGCCATCAGTGAAACCTGTGTGGGAAAGAGAAAAACAAGTTGGAACAATAACTACACAACCATTTCCACAATGTCCCATGGACAGAAAATCTGTTACATTTTCACCCTTTGTGAATCAAAATAATACTATGATTATGAAACAATATTGTTTTAAAGTAAAGGGTGAAATGATAACAAGACTTAAGATGAGTTTGATGAATGAAAATGAAAACATAAGGTTCACAACTTTTGAAGTGCTTGCAGGTTATGTGTGGAGGTCAAGATCAAGAGCCTTAAAACTTAGCAGCAATGACAAAACAATGCTAAATGTTTTAGTTGGTATCAGACGAAACTTGATGGATTATGATACTTTACCTAAAGGGTATTATGGCAATTCTACCATAGATGCAAAAGTTGTTTTAAAAGTGAGTGAGCTCAACGAAATGCCACTCTATGAAACCGTTAAGCTGATTAAAGAGACTAAAAATATTGCTTTCACTGCGGATTATGTCACAAACTCAATAAACTCTTTGGAGACGAATCAAGAGGATGGTTTGAGCATGGAATTGGAAGCTAGTGGTGCAGTAACGGTTCTAACGGAGTGGAAACATTTGGGTTTTCAGGAAAATTTAGATTTTGGAGGCTATGAATTAGTGAATTTTCTACCTGCACCATGCAAGATGCTTGCCACCATCGATACGTGTATTTTCTCATCTCCTAATAAACTTGATGATCATGATCCATCAATGGATGGAGGAGTTAGGATATTTACATCACTCCCTGTTGCTGCTATGCCTATGTTCAGGGATGAAATTGAAGCTTTAAGGTTTCTTTGTTGAAATTAAAGTGGATTTGGATATGGTGGCTGGTGCAGTGGGCTGTTAAGTCTTTTGATTAACCGAAGACAATCGCTCTTTATGCACTTAAAATTCACTCGTGATTGGATCTTAACTCCCGATTGACAATTTTTGTGTGTAAAATCCGATTGGATTGTAACTTCAGAAGCAATTGTCTTAACTGAATGCAAGTAAGATTCAAAGACTCAACGGATCGAATTTCATTCTTTTCCATTTCATTTGTACCTTATCCCTAATTTCTGTTTTATAGTATTGTCATGACTTGTCATCATTAATTGTTTTTTTAATTATATATATCCTCTTTGGATTATTGTACAACTGTGTTATTTTTATCATGAAATGTTATAAGGAAGATTCTATTTGTAGCCTCGATACATTATTTCATTAAACAACAAGGATTATAGACATAATGAAAGTATTAGGGGTATTCTAGCCCAAACAAACAAGATATTCACAAATCCAACCAACAACAAGAGAGGCTTATCAATCAAGACCAAATCCGAAAAGAACAAGGAGAAATTAGATTGATGGGGTGGTTTCGGCGAGGTTTTTGCAATTTTATAGCTGGGTTGAATACTACTTGTACTTATTGTCGCTTGAATGACTAAATTTTTGTTTGATGTCAAAAAACAAATCGCCAATTCTTAGAGAGATTTTTTTTTTAATGGAATTCTTAGAGAGACCGACAAAAGAAGTCACCCATCATTCATAATACAAGCAATGCACACATTCTCCCTAACCAAAAATCATTTTCAAGAGAGGAGCTTGATAGTTTGAAAAATTAGAGTAACTAATTTGCAATTTTTTAAAAATTTATGTGGACGGATTTGCAGTTTTGTAATATTTGTAGTTTATAATGTGTAAAAGGCACTTCATCATCTTTTCCTTAAACCAACAAATTTTTAACAACATTGACTTCCTTCTAAAGAGTCGGGTACCTATGAATTGTGTGAGTATTTGGTAAAGGTTGCACAATTGGAGCTTCTAAATTCGTCCCAGGAGATGATTGCGCAATTGCATTAGCTGCTATTATATTCTCAACCCTACTAGATGCTTCCATATTTATATTCTCAACCCTCCTCCTACCTTAACCTCTAGCCTTTTCCTATAAGGAGTATGCAAGGTTATTTTTGACAGCCTCCAAGAGCTAGTGGAAGCAAGACATCAGGCTATTCACCTTGTGAACTATGCTGATAAGTGGCAGAATCTAAGGCAGTTGGTGAATAGAACCTTGGATGATCTTCAAACAATTGCTATAGCTTCTTAGCAAGAAGCTCTCAGAAGCTGGTTTGAAGAAGTCTGCAGAAGCATCGAGGAAGTGGAACTGAAGAAAGACATCACCAGACAGATTTCAGACTCTAAATTTGTTGAAGCAAATCCTTCAGAAGTTCCTAAAATTTTGCAATCTGGACTTCTAGCTTTTTGCTCTCTAATAAGTTCTTTGTGAAACCTGGTCAGATAGTGATAAAAAGTCAAGAACCTGAGATGATTCAGTTTCAAGACAAAGTGGAAGCTGGTCTGCAAGAACAGAAGATGACTACAAAAGCTATGAAAGCTCAGCTAATGATAGTTATGAAAGAGCAGAAACGTCTGAGTGATAATCTTGTTCAAATAGTCAGAAGACAAGAAGAGCTGGCTAGATAA

Protein sequence

>MS.gene38527.t1

MEYEKATLKIEKEDVLLVKPSKSIPSCILSLSTLDNKGANNNIALTVHVYRSSSAIHDYHSSFNPCHVFKEALSKALFYYYPLAGRLVRHADGKFRVNANTNGTEFGVPFLEAAANCTLSSLHYLDNTNTEIAKHLVLDIPSPKDKSYPLVLMVTKFLCGGFTIGMGMSHAICDGFGASQFFKAVVELASGRTEPSVKPVWEREKQVGTITTQPFPQCPMDRKSVTFSPFVNQNNTMIMKQYCFKVKGEMITRLKMSLMNENENIRFTTFEVLAGYVWRSRSRALKLSSNDKTMLNVLVGIRRNLMDYDTLPKGYYGNSTIDAKVVLKVSELNEMPLYETVKLIKETKNIAFTADYVTNSINSLETNQEDGLSMELEASGAVTVLTEWKHLGFQENLDFGGYELVNFLPAPCKMLATIDTCIFSSPNKLDDHDPSMDGGVRIFTSLPVAAMPMFRDEIEALSFLLSNKFFVKPGQIVIKSQEPEMIQFQDKVEAGLQEQKMTTKAMKAQLMIVMKEQKRLSDNLVQIVRRQEELAR