AlfalfaGEDB | MS.gene78200

M. sativa cultivar XinJiangDaYe / MS.gene78200

KEGG: K10956
GO: GO:0005575 GO:0006810 GO:0008104 GO:0008150 GO:0015031 GO:0015833 GO:0016020 GO:0033036 GO:0042886 GO:0045184 GO:0051179 GO:0051234 GO:0071702 GO:0071705 GO:0110165
PFAM: PF00344.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78200.t1	KEH38065.1	90.8	531	37	3	1	524	21	546	3.60E-255	890.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78200.t1	Q9XQU4	82.7	532	78	3	2	524	1	527	1.1e-233	810.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78200.t1	A0A072VIS8	90.8	531	37	3	1	524	21	546	2.6e-255	890.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049137	MS.gene78200	0.812604	3.90E-51	-1.69E-46
MS.gene049150	MS.gene78200	0.811454	6.95E-51	-1.69E-46
MS.gene049153	MS.gene78200	0.809529	1.81E-50	-1.69E-46
MS.gene049346	MS.gene78200	0.812358	4.41E-51	-1.69E-46
MS.gene049351	MS.gene78200	0.869942	2.12E-66	-1.69E-46
MS.gene049356	MS.gene78200	0.835279	1.84E-56	-1.69E-46
MS.gene049369	MS.gene78200	0.814628	1.40E-51	-1.69E-46
MS.gene049389	MS.gene78200	0.828761	7.51E-55	-1.69E-46
MS.gene049427	MS.gene78200	0.879971	8.06E-70	-1.69E-46
MS.gene049491	MS.gene78200	0.819574	1.08E-52	-1.69E-46
MS.gene049661	MS.gene78200	0.850184	2.00E-60	-1.69E-46
MS.gene049662	MS.gene78200	0.845806	3.23E-59	-1.69E-46
MS.gene049758	MS.gene78200	0.852854	3.51E-61	-1.69E-46
MS.gene049805	MS.gene78200	0.82226	2.60E-53	-1.69E-46
MS.gene049910	MS.gene78200	0.800553	1.37E-48	-1.69E-46
MS.gene050076	MS.gene78200	0.819104	1.38E-52	-1.69E-46
MS.gene050099	MS.gene78200	0.813149	2.96E-51	-1.69E-46
MS.gene050128	MS.gene78200	0.822604	2.17E-53	-1.69E-46
MS.gene050301	MS.gene78200	0.923221	3.53E-89	-1.69E-46
MS.gene050397	MS.gene78200	0.813925	2.00E-51	-1.69E-46
MS.gene050406	MS.gene78200	0.812369	4.39E-51	-1.69E-46
MS.gene050432	MS.gene78200	0.848057	7.81E-60	-1.69E-46
MS.gene050464	MS.gene78200	0.835618	1.51E-56	-1.69E-46
MS.gene050529	MS.gene78200	0.84651	2.08E-59	-1.69E-46
MS.gene050593	MS.gene78200	0.81696	4.22E-52	-1.69E-46
MS.gene050670	MS.gene78200	0.893427	6.35E-75	-1.69E-46
MS.gene050767	MS.gene78200	0.815581	8.59E-52	-1.69E-46
MS.gene050770	MS.gene78200	0.819736	9.93E-53	-1.69E-46
MS.gene050783	MS.gene78200	0.871878	4.88E-67	-1.69E-46
MS.gene050812	MS.gene78200	0.893279	7.29E-75	-1.69E-46
MS.gene05101	MS.gene78200	0.876666	1.16E-68	-1.69E-46
MS.gene05105	MS.gene78200	0.852859	3.50E-61	-1.69E-46
MS.gene051387	MS.gene78200	0.860417	2.10E-63	-1.69E-46
MS.gene051471	MS.gene78200	0.86534	6.34E-65	-1.69E-46
MS.gene051525	MS.gene78200	0.829413	5.22E-55	-1.69E-46
MS.gene051582	MS.gene78200	0.896073	5.23E-76	-1.69E-46
MS.gene051663	MS.gene78200	0.815503	8.94E-52	-1.69E-46
MS.gene051666	MS.gene78200	0.836544	8.79E-57	-1.69E-46
MS.gene051860	MS.gene78200	0.820128	8.08E-53	-1.69E-46
MS.gene051877	MS.gene78200	0.807187	5.71E-50	-1.69E-46
MS.gene052011	MS.gene78200	-0.819316	1.24E-52	-1.69E-46
MS.gene052078	MS.gene78200	0.85031	1.84E-60	-1.69E-46
MS.gene052178	MS.gene78200	0.821446	4.02E-53	-1.69E-46
MS.gene052188	MS.gene78200	0.902713	7.31E-79	-1.69E-46
MS.gene052207	MS.gene78200	0.80727	5.49E-50	-1.69E-46
MS.gene052253	MS.gene78200	0.866579	2.57E-65	-1.69E-46
MS.gene052310	MS.gene78200	0.889048	3.43E-73	-1.69E-46
MS.gene052322	MS.gene78200	0.839417	1.60E-57	-1.69E-46
MS.gene052326	MS.gene78200	0.861333	1.10E-63	-1.69E-46
MS.gene052340	MS.gene78200	0.874605	5.93E-68	-1.69E-46
MS.gene052414	MS.gene78200	0.803501	3.38E-49	-1.69E-46
MS.gene052421	MS.gene78200	0.841375	4.94E-58	-1.69E-46
MS.gene052481	MS.gene78200	0.840478	8.49E-58	-1.69E-46
MS.gene052484	MS.gene78200	0.824507	7.77E-54	-1.69E-46
MS.gene052485	MS.gene78200	0.884688	1.55E-71	-1.69E-46
MS.gene052982	MS.gene78200	0.833385	5.50E-56	-1.69E-46
MS.gene053103	MS.gene78200	0.815104	1.10E-51	-1.69E-46
MS.gene053107	MS.gene78200	0.853025	3.14E-61	-1.69E-46
MS.gene053207	MS.gene78200	0.800026	1.75E-48	-1.69E-46
MS.gene053209	MS.gene78200	0.835554	1.57E-56	-1.69E-46
MS.gene053322	MS.gene78200	0.833107	6.45E-56	-1.69E-46
MS.gene053557	MS.gene78200	0.840728	7.30E-58	-1.69E-46
MS.gene053579	MS.gene78200	0.80661	7.57E-50	-1.69E-46
MS.gene053813	MS.gene78200	0.803605	3.22E-49	-1.69E-46
MS.gene053836	MS.gene78200	0.871865	4.93E-67	-1.69E-46
MS.gene053907	MS.gene78200	0.82562	4.24E-54	-1.69E-46
MS.gene053928	MS.gene78200	0.86848	6.33E-66	-1.69E-46
MS.gene054010	MS.gene78200	0.810919	9.07E-51	-1.69E-46
MS.gene054011	MS.gene78200	0.802094	6.60E-49	-1.69E-46
MS.gene054123	MS.gene78200	0.912647	1.53E-83	-1.69E-46
MS.gene054183	MS.gene78200	0.818445	1.95E-52	-1.69E-46
MS.gene054376	MS.gene78200	0.835627	1.50E-56	-1.69E-46
MS.gene054556	MS.gene78200	0.801251	9.83E-49	-1.69E-46
MS.gene05455	MS.gene78200	0.879382	1.30E-69	-1.69E-46
MS.gene054719	MS.gene78200	0.80883	2.55E-50	-1.69E-46
MS.gene054802	MS.gene78200	0.835865	1.31E-56	-1.69E-46
MS.gene054803	MS.gene78200	0.835128	2.01E-56	-1.69E-46
MS.gene054804	MS.gene78200	0.836613	8.45E-57	-1.69E-46
MS.gene054955	MS.gene78200	0.817017	4.10E-52	-1.69E-46
MS.gene05505	MS.gene78200	0.801696	7.97E-49	-1.69E-46
MS.gene055116	MS.gene78200	0.884746	1.47E-71	-1.69E-46
MS.gene055277	MS.gene78200	0.853326	2.57E-61	-1.69E-46
MS.gene055369	MS.gene78200	0.836947	6.94E-57	-1.69E-46
MS.gene055390	MS.gene78200	0.890952	6.18E-74	-1.69E-46
MS.gene055412	MS.gene78200	0.854472	1.20E-61	-1.69E-46
MS.gene055422	MS.gene78200	0.881451	2.37E-70	-1.69E-46
MS.gene055428	MS.gene78200	0.862735	4.10E-64	-1.69E-46
MS.gene055499	MS.gene78200	0.815036	1.14E-51	-1.69E-46
MS.gene055533	MS.gene78200	0.803812	2.92E-49	-1.69E-46
MS.gene055539	MS.gene78200	0.825904	3.63E-54	-1.69E-46
MS.gene05553	MS.gene78200	0.863219	2.91E-64	-1.69E-46
MS.gene055564	MS.gene78200	0.852903	3.40E-61	-1.69E-46
MS.gene055604	MS.gene78200	0.840032	1.11E-57	-1.69E-46
MS.gene055712	MS.gene78200	0.841023	6.11E-58	-1.69E-46
MS.gene055896	MS.gene78200	0.804477	2.12E-49	-1.69E-46
MS.gene055930	MS.gene78200	0.805232	1.47E-49	-1.69E-46
MS.gene05594	MS.gene78200	0.827339	1.65E-54	-1.69E-46
MS.gene05602	MS.gene78200	0.823656	1.23E-53	-1.69E-46
MS.gene056191	MS.gene78200	0.834368	3.12E-56	-1.69E-46
MS.gene056407	MS.gene78200	0.915338	6.63E-85	-1.69E-46
MS.gene056410	MS.gene78200	0.841968	3.44E-58	-1.69E-46
MS.gene056497	MS.gene78200	0.809548	1.79E-50	-1.69E-46
MS.gene056511	MS.gene78200	0.812821	3.50E-51	-1.69E-46
MS.gene056609	MS.gene78200	0.826998	1.99E-54	-1.69E-46
MS.gene056612	MS.gene78200	0.861607	9.11E-64	-1.69E-46
MS.gene056659	MS.gene78200	0.845796	3.25E-59	-1.69E-46
MS.gene056723	MS.gene78200	0.860812	1.59E-63	-1.69E-46
MS.gene056796	MS.gene78200	0.840527	8.24E-58	-1.69E-46
MS.gene056897	MS.gene78200	0.819113	1.38E-52	-1.69E-46
MS.gene056899	MS.gene78200	-0.814388	1.58E-51	-1.69E-46
MS.gene056902	MS.gene78200	0.814851	1.25E-51	-1.69E-46
MS.gene057011	MS.gene78200	0.871073	9.02E-67	-1.69E-46
MS.gene05702	MS.gene78200	0.863419	2.52E-64	-1.69E-46
MS.gene057121	MS.gene78200	0.87681	1.04E-68	-1.69E-46
MS.gene057124	MS.gene78200	0.884995	1.19E-71	-1.69E-46
MS.gene057129	MS.gene78200	0.819633	1.05E-52	-1.69E-46
MS.gene057146	MS.gene78200	0.839427	1.60E-57	-1.69E-46
MS.gene057245	MS.gene78200	0.828326	9.57E-55	-1.69E-46
MS.gene057295	MS.gene78200	0.826816	2.20E-54	-1.69E-46
MS.gene05735	MS.gene78200	0.815773	7.79E-52	-1.69E-46
MS.gene057396	MS.gene78200	0.845099	5.02E-59	-1.69E-46
MS.gene057416	MS.gene78200	0.807842	4.15E-50	-1.69E-46
MS.gene057439	MS.gene78200	0.800259	1.57E-48	-1.69E-46
MS.gene057448	MS.gene78200	0.871084	8.94E-67	-1.69E-46
MS.gene057449	MS.gene78200	0.860685	1.74E-63	-1.69E-46
MS.gene057450	MS.gene78200	0.874589	6.00E-68	-1.69E-46
MS.gene057469	MS.gene78200	0.815794	7.70E-52	-1.69E-46
MS.gene057537	MS.gene78200	0.809632	1.72E-50	-1.69E-46
MS.gene057749	MS.gene78200	0.852465	4.54E-61	-1.69E-46
MS.gene057823	MS.gene78200	0.853715	1.99E-61	-1.69E-46
MS.gene057825	MS.gene78200	0.812428	4.26E-51	-1.69E-46
MS.gene058002	MS.gene78200	0.901395	2.80E-78	-1.69E-46
MS.gene058007	MS.gene78200	0.843828	1.10E-58	-1.69E-46
MS.gene058019	MS.gene78200	0.844364	7.92E-59	-1.69E-46
MS.gene058082	MS.gene78200	0.845824	3.19E-59	-1.69E-46
MS.gene05811	MS.gene78200	0.869498	2.96E-66	-1.69E-46
MS.gene058142	MS.gene78200	0.866173	3.46E-65	-1.69E-46
MS.gene058233	MS.gene78200	0.853209	2.78E-61	-1.69E-46
MS.gene058276	MS.gene78200	0.840465	8.56E-58	-1.69E-46
MS.gene058293	MS.gene78200	0.907009	8.04E-81	-1.69E-46
MS.gene058323	MS.gene78200	0.81058	1.07E-50	-1.69E-46
MS.gene058365	MS.gene78200	0.877963	4.12E-69	-1.69E-46
MS.gene05842	MS.gene78200	0.831963	1.24E-55	-1.69E-46
MS.gene058660	MS.gene78200	0.906426	1.50E-80	-1.69E-46
MS.gene058700	MS.gene78200	0.85961	3.67E-63	-1.69E-46
MS.gene058787	MS.gene78200	0.86606	3.76E-65	-1.69E-46
MS.gene058845	MS.gene78200	0.819494	1.13E-52	-1.69E-46
MS.gene05886	MS.gene78200	0.850407	1.73E-60	-1.69E-46
MS.gene058928	MS.gene78200	0.831707	1.43E-55	-1.69E-46
MS.gene058948	MS.gene78200	0.823545	1.31E-53	-1.69E-46
MS.gene058949	MS.gene78200	0.836757	7.76E-57	-1.69E-46
MS.gene058993	MS.gene78200	0.832197	1.08E-55	-1.69E-46
MS.gene059011	MS.gene78200	0.813156	2.95E-51	-1.69E-46
MS.gene05907	MS.gene78200	0.897827	9.64E-77	-1.69E-46
MS.gene059141	MS.gene78200	0.842852	2.01E-58	-1.69E-46
MS.gene059342	MS.gene78200	0.819488	1.13E-52	-1.69E-46
MS.gene059366	MS.gene78200	0.815124	1.09E-51	-1.69E-46
MS.gene059420	MS.gene78200	0.867568	1.24E-65	-1.69E-46
MS.gene059462	MS.gene78200	0.81304	3.13E-51	-1.69E-46
MS.gene059518	MS.gene78200	0.825288	5.09E-54	-1.69E-46
MS.gene059536	MS.gene78200	0.844668	6.56E-59	-1.69E-46
MS.gene059597	MS.gene78200	0.870136	1.83E-66	-1.69E-46
MS.gene05959	MS.gene78200	0.862778	3.98E-64	-1.69E-46
MS.gene059676	MS.gene78200	0.897082	1.98E-76	-1.69E-46
MS.gene059688	MS.gene78200	0.894329	2.73E-75	-1.69E-46
MS.gene059692	MS.gene78200	0.892208	1.96E-74	-1.69E-46
MS.gene059695	MS.gene78200	0.898	8.14E-77	-1.69E-46
MS.gene059708	MS.gene78200	0.841073	5.93E-58	-1.69E-46
MS.gene05984	MS.gene78200	0.841329	5.08E-58	-1.69E-46
MS.gene059894	MS.gene78200	0.843418	1.42E-58	-1.69E-46
MS.gene059899	MS.gene78200	0.809615	1.73E-50	-1.69E-46
MS.gene059960	MS.gene78200	0.860094	2.62E-63	-1.69E-46
MS.gene060193	MS.gene78200	0.81303	3.15E-51	-1.69E-46
MS.gene06037	MS.gene78200	0.826205	3.08E-54	-1.69E-46
MS.gene060403	MS.gene78200	0.857654	1.41E-62	-1.69E-46
MS.gene060407	MS.gene78200	0.871135	8.61E-67	-1.69E-46
MS.gene060804	MS.gene78200	0.82422	9.08E-54	-1.69E-46
MS.gene060837	MS.gene78200	0.817286	3.57E-52	-1.69E-46
MS.gene06090	MS.gene78200	0.85467	1.05E-61	-1.69E-46
MS.gene060971	MS.gene78200	0.812997	3.20E-51	-1.69E-46
MS.gene061049	MS.gene78200	0.875663	2.58E-68	-1.69E-46
MS.gene061051	MS.gene78200	-0.871727	5.48E-67	-1.69E-46
MS.gene061154	MS.gene78200	0.849907	2.39E-60	-1.69E-46
MS.gene061159	MS.gene78200	0.838095	3.52E-57	-1.69E-46
MS.gene061200	MS.gene78200	0.851754	7.22E-61	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78200.t1	MTR_2g460600	96.798	531	5	3	1	524	21	546	0.0	1007
MS.gene78200.t1	MTR_7g094800	27.962	422	273	13	108	506	118	531	1.11e-34	137

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78200.t1	AT2G18710	83.058	484	75	3	48	524	66	549	0.0	799
MS.gene78200.t1	AT2G31530	28.235	425	267	13	108	506	147	559	7.26e-33	132

Find 102 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCTGCTTACCTACCGTTTA+AGG	0.117513	2.2:-32907690	MS.gene78200:intron
GAGGATAGTTCAATTGATTT+TGG	0.223790	2.2:-32909364	MS.gene78200:CDS
TGCACACTTGACTGCATTCC+GGG	0.270529	2.2:-32906766	MS.gene78200:CDS
AGGTTCCATTCCCAAGTTTC+AGG	0.271250	2.2:+32908013	None:intergenic
TCTTACTTGCCAGCTTCTTT+TGG	0.293036	2.2:-32907964	MS.gene78200:CDS
AATCTAGACGCGTAGTTAAT+AGG	0.304266	2.2:+32907740	None:intergenic
TCTTAGTCGAATATCAGTTC+TGG	0.312877	2.2:-32906841	MS.gene78200:CDS
ACTGCATTCCGGGGATTTGC+TGG	0.320442	2.2:-32906756	MS.gene78200:CDS
GCTATCAAAAGTATGTTAAA+TGG	0.328201	2.2:+32907117	None:intergenic
ACATCTATTCTTATTCTTGT+TGG	0.328592	2.2:-32906732	MS.gene78200:CDS
CTGCACACTTGACTGCATTC+CGG	0.330585	2.2:-32906767	MS.gene78200:CDS
AATTGTTCGCTCACATCATC+TGG	0.339654	2.2:+32907057	None:intergenic
CTATTACACTTTCCTACAAC+TGG	0.344454	2.2:-32907082	MS.gene78200:CDS
TCCTTCTTTCTGTTGGTAGT+TGG	0.350967	2.2:-32907880	MS.gene78200:CDS
ACATCATCGTTAGCTCTTCC+TGG	0.375335	2.2:-32907489	MS.gene78200:CDS
GCAGTCTGCTCAACAATTGC+AGG	0.392772	2.2:+32906786	None:intergenic
CAAATTACTGACCTGAAACT+TGG	0.408846	2.2:-32908024	MS.gene78200:CDS
CCGTGATTACAACGTACATT+GGG	0.408942	2.2:-32908050	MS.gene78200:CDS
CTTAGTCGAATATCAGTTCT+GGG	0.412406	2.2:-32906840	MS.gene78200:CDS
TTCTGAAGATCTTGCAACTT+GGG	0.415468	2.2:+32909085	None:intergenic
TTCTTCAAAGGTCCTCTGCC+AGG	0.418038	2.2:-32909337	MS.gene78200:CDS
CATCATCTGGATCCAGTTGT+AGG	0.418316	2.2:+32907070	None:intergenic
CATTGTGTCCTTCTTTCTGT+TGG	0.429476	2.2:-32907887	MS.gene78200:CDS
AGTTGGAATTGTCTATGTTC+AGG	0.434605	2.2:-32907863	MS.gene78200:intron
TTTGGCAGGTCAATAGTTCT+GGG	0.435453	2.2:-32907533	MS.gene78200:intron
CTTCTCTCCGGTGGTGTGTC+CGG	0.444100	2.2:-32909709	MS.gene78200:CDS
AAACCTCGAGGAGGAGGAGG+AGG	0.445397	2.2:-32909409	MS.gene78200:CDS
AGGAGTAGCAGCTGCAATAG+AGG	0.448637	2.2:-32909383	MS.gene78200:CDS
AGACTTGGCATATGCTCTCT+TGG	0.449449	2.2:-32909163	MS.gene78200:CDS
ACCAATACCACCACCAGAAA+AGG	0.461774	2.2:+32909186	None:intergenic
TTTCTTGTATTGTCAAGGCT+TGG	0.462644	2.2:-32909292	MS.gene78200:CDS
CGGACACACCACCGGAGAGA+AGG	0.462910	2.2:+32909710	None:intergenic
GGACACACCACCGGAGAGAA+GGG	0.465536	2.2:+32909711	None:intergenic
TGTTGGATTCGCCATTGTAC+AGG	0.467660	2.2:-32909008	MS.gene78200:intron
TACACTCGATATGCTTCTGT+TGG	0.467789	2.2:-32909025	MS.gene78200:CDS
TAGGGTTTCTTGTATTGTCA+AGG	0.468985	2.2:-32909297	MS.gene78200:CDS
GATGATGTAGAAAATATGAT+AGG	0.473627	2.2:+32907504	None:intergenic
GGTACTTTAGCACGTTTCAC+TGG	0.478650	2.2:-32907468	MS.gene78200:CDS
AAAGATGAGAGGACCTTAAA+CGG	0.479005	2.2:+32907677	None:intergenic
TTTCTGAAGATCTTGCAACT+TGG	0.479036	2.2:+32909084	None:intergenic
AGAGAGAGTGGCTCTGCAAA+TGG	0.479809	2.2:+32909655	None:intergenic
TATGTTAAATGGAAGATAGA+AGG	0.481243	2.2:+32907128	None:intergenic
TGTTGTAAAGTCATTTACAT+AGG	0.488090	2.2:+32908113	None:intergenic
TCTGTTATCTTATTGACACT+TGG	0.501427	2.2:-32908075	MS.gene78200:CDS
CAGAAAAGAGAAGGTGAGGC+TGG	0.502761	2.2:-32909067	MS.gene78200:CDS
AAGAAGAGTTAATACCTAAC+GGG	0.503434	2.2:+32909488	None:intergenic
TGAGAGGACGATTATTAAGC+CGG	0.504157	2.2:+32909690	None:intergenic
AGTGTAATAGTAGTTGAAGA+AGG	0.517143	2.2:+32907095	None:intergenic
GCAGAGCCACTCTCTCTGTC+CGG	0.519848	2.2:-32909649	MS.gene78200:CDS
TCCGTGATTACAACGTACAT+TGG	0.519948	2.2:-32908051	MS.gene78200:CDS
TTGAGAAACTTCCCTGGCAG+AGG	0.521457	2.2:+32909325	None:intergenic
GTAGCGAATTTCGACCCGTT+AGG	0.521598	2.2:-32909502	MS.gene78200:CDS
GCTGCAGTAGCTTTGAATCC+AGG	0.527425	2.2:-32907426	MS.gene78200:CDS
AATGTAGCTGTACTTCTGCC+TGG	0.528569	2.2:+32907000	None:intergenic
GTTGTAAAGTCATTTACATA+GGG	0.531063	2.2:+32908114	None:intergenic
AAATTACTGACCTGAAACTT+GGG	0.532762	2.2:-32908023	MS.gene78200:CDS
GGTGGTGGTATTGGTAGACT+TGG	0.533736	2.2:-32909178	MS.gene78200:CDS
CTCAACCTCATCTCTCTACC+AGG	0.538600	2.2:-32909613	MS.gene78200:intron
CCCAATGTACGTTGTAATCA+CGG	0.539847	2.2:+32908050	None:intergenic
TCCAACTACCAACAGAAAGA+AGG	0.539872	2.2:+32907879	None:intergenic
AAGAAGCTTCCCCTTCTCTC+CGG	0.545920	2.2:-32909721	MS.gene78200:CDS
CTTGGTGTGTATATACCTCT+AGG	0.546349	2.2:-32909274	MS.gene78200:CDS
TATTAAGCCGGACACACCAC+CGG	0.551111	2.2:+32909702	None:intergenic
CAGAAAGAAGGACACAATGA+TGG	0.551543	2.2:+32907891	None:intergenic
GAAGAAGAGTTAATACCTAA+CGG	0.552203	2.2:+32909487	None:intergenic
ACTGACCTGAAACTTGGGAA+TGG	0.555203	2.2:-32908018	MS.gene78200:CDS
ACAATCTGTGCATTGATGAA+AGG	0.557062	2.2:+32909133	None:intergenic
GACACACCACCGGAGAGAAG+GGG	0.559651	2.2:+32909712	None:intergenic
AATGTCCGACCAAAAGAAGC+TGG	0.561861	2.2:+32907955	None:intergenic
GGTAACAAGTCCGACATAGT+TGG	0.563139	2.2:+32907912	None:intergenic
GTTGAGTGGTAGATATTCTC+CGG	0.567019	2.2:+32909630	None:intergenic
TCTTCAGAAAAGAGAAGGTG+AGG	0.569860	2.2:-32909071	MS.gene78200:CDS
CAAGATCTTCAGAAAAGAGA+AGG	0.570295	2.2:-32909076	MS.gene78200:CDS
TCAATTCCACTTGTCAGACC+AGG	0.572539	2.2:-32907018	MS.gene78200:CDS
TTTAATGATGCCAACTATGT+CGG	0.578808	2.2:-32907922	MS.gene78200:CDS
GCACACTTGACTGCATTCCG+GGG	0.581736	2.2:-32906765	MS.gene78200:CDS
TAGATGTTCCAGCAAATCCC+CGG	0.583086	2.2:+32906748	None:intergenic
TCTTCAAAGGTCCTCTGCCA+GGG	0.584956	2.2:-32909336	MS.gene78200:CDS
CTTCTGCCTGGTCTGACAAG+TGG	0.593403	2.2:+32907012	None:intergenic
AAGCTTCCCCTTCTCTCCGG+TGG	0.593508	2.2:-32909718	MS.gene78200:CDS
AGCGAACAATTGAAACGTCA+AGG	0.598941	2.2:-32907045	MS.gene78200:CDS
GTTTGTGAATATCAAAAGAG+AGG	0.599776	2.2:+32907993	None:intergenic
ATGGAAGAAGATGGTTTGAG+AGG	0.600121	2.2:+32909674	None:intergenic
AGGGACGCAGGAAAAGAACT+TGG	0.600736	2.2:+32908133	None:intergenic
ATGAGAGGACCTTAAACGGT+AGG	0.606091	2.2:+32907681	None:intergenic
TAAATTTCTGACCTGTACAA+TGG	0.607947	2.2:+32908997	None:intergenic
TCTAGATTCACCAGCAAAAG+CGG	0.612020	2.2:-32907725	MS.gene78200:CDS
GTGAAACGTGCTAAAGTACC+AGG	0.612842	2.2:+32907471	None:intergenic
AAAAGCTTGAGAAACTTCCC+TGG	0.613913	2.2:+32909319	None:intergenic
TATTCTCCGGACAGAGAGAG+TGG	0.622305	2.2:+32909643	None:intergenic
GGTAGAGAGATGAGGTTGAG+TGG	0.627551	2.2:+32909616	None:intergenic
AAATGACTTTACAACAGAGT+GGG	0.636424	2.2:-32908106	MS.gene78200:CDS
TTGGCAGGTCAATAGTTCTG+GGG	0.643969	2.2:-32907532	MS.gene78200:intron
AGTCATTTACATAGGGACGC+AGG	0.644501	2.2:+32908121	None:intergenic
GCTCTGCAAATGGAAGAAGA+TGG	0.652952	2.2:+32909665	None:intergenic
TAAATGACTTTACAACAGAG+TGG	0.657979	2.2:-32908107	MS.gene78200:CDS
AGATTCACCAGCAAAAGCGG+TGG	0.659232	2.2:-32907722	MS.gene78200:CDS
CGTGATTACAACGTACATTG+GGG	0.663302	2.2:-32908049	MS.gene78200:CDS
GGTGTGTATATACCTCTAGG+TGG	0.698321	2.2:-32909271	MS.gene78200:CDS
GCAGTAGCTTTGAATCCAGG+AGG	0.714613	2.2:-32907423	MS.gene78200:intron
TCTCTGTTAACTCCACCTAG+AGG	0.718674	2.2:+32909259	None:intergenic
TCTAGGTGGAGTTAACAGAG+AGG	0.738479	2.2:-32909257	MS.gene78200:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAATATAAAATATAATATA+TGG	-	chr2.2:32909510-32909529	MS.gene78200:CDS	0.0%
!!	AAAAATGTACATTTAAGTAA+AGG	+	chr2.2:32909222-32909241	None:intergenic	15.0%
!!	AAAATGTACATTTAAGTAAA+GGG	+	chr2.2:32909221-32909240	None:intergenic	15.0%
!!	ACAAAAACAAAATTATAAAC+TGG	+	chr2.2:32908201-32908220	None:intergenic	15.0%
!!	TTGATTTCAAAATTAATTCA+TGG	+	chr2.2:32907838-32907857	None:intergenic	15.0%
!!!	AAATGTACATTTTTTTTTGT+AGG	-	chr2.2:32909228-32909247	MS.gene78200:CDS	15.0%
!!!	ATGAATTAATTTTGAAATCA+AGG	-	chr2.2:32907837-32907856	MS.gene78200:intron	15.0%
!!!	CAATTTTATTATTATAAACC+CGG	+	chr2.2:32909002-32909021	None:intergenic	15.0%
!!!	TGAATTAATTTTGAAATCAA+GGG	-	chr2.2:32907838-32907857	MS.gene78200:intron	15.0%
!!	AAACTTTGAATAAATCATGA+AGG	+	chr2.2:32907662-32907681	None:intergenic	20.0%
!!	AGAAATGTTTGATGAAATAT+AGG	-	chr2.2:32909114-32909133	MS.gene78200:CDS	20.0%
!!	AGTAGAAAAAGATTTATGTT+AGG	-	chr2.2:32907790-32907809	MS.gene78200:intron	20.0%
!!	ATATTGTAATTACTGCATTT+AGG	-	chr2.2:32907917-32907936	MS.gene78200:CDS	20.0%
!!	ATTAAGTGATGTAAAATGAA+TGG	+	chr2.2:32909495-32909514	None:intergenic	20.0%
!!	GTAGAAAAAGATTTATGTTA+GGG	-	chr2.2:32907791-32907810	MS.gene78200:intron	20.0%
!!	TAAAATATAATATATGGTGC+AGG	-	chr2.2:32909516-32909535	MS.gene78200:intron	20.0%
!!	TATTGTAATTACTGCATTTA+GGG	-	chr2.2:32907918-32907937	MS.gene78200:CDS	20.0%
!!	TATTTGAATTCATGAAGAAA+AGG	+	chr2.2:32907474-32907493	None:intergenic	20.0%
!	AACATCTACATTGTCAAATT+TGG	+	chr2.2:32908824-32908843	None:intergenic	25.0%
!	AAGTAAAGGGAATCAATTTA+TGG	+	chr2.2:32909208-32909227	None:intergenic	25.0%
!	ACATCTATTCTTATTCTTGT+TGG	-	chr2.2:32909649-32909668	MS.gene78200:CDS	25.0%
!	AGTAATTACAATATAGAGGA+GGG	+	chr2.2:32907911-32907930	None:intergenic	25.0%
!	ATACAATCCATTTGAAAATG+AGG	+	chr2.2:32906848-32906867	None:intergenic	25.0%
!	ATGCAGTAATTACAATATAG+AGG	+	chr2.2:32907915-32907934	None:intergenic	25.0%
!	ATTAATCAGTAAGGAAATCA+AGG	+	chr2.2:32909448-32909467	None:intergenic	25.0%
!	ATTTGAAAATGAGGCAAAAA+TGG	+	chr2.2:32906839-32906858	None:intergenic	25.0%
!	CTTGTTGAGATAATGTTAAA+AGG	-	chr2.2:32907712-32907731	MS.gene78200:CDS	25.0%
!	GATGATGTAGAAAATATGAT+AGG	+	chr2.2:32908880-32908899	None:intergenic	25.0%
!	GTACTACAGTAACATAAAAA+AGG	+	chr2.2:32908736-32908755	None:intergenic	25.0%
!	GTTGTAAAGTCATTTACATA+GGG	+	chr2.2:32908270-32908289	None:intergenic	25.0%
!	TACAGTAACATAAAAAAGGT+TGG	+	chr2.2:32908732-32908751	None:intergenic	25.0%
!	TATAACCAAGAGTCATTAAT+AGG	+	chr2.2:32907958-32907977	None:intergenic	25.0%
!	TATCAAATACTATTCGAGTA+AGG	+	chr2.2:32908758-32908777	None:intergenic	25.0%
!	TATGTTAAATGGAAGATAGA+AGG	+	chr2.2:32909256-32909275	None:intergenic	25.0%
!	TGTTGTAAAGTCATTTACAT+AGG	+	chr2.2:32908271-32908290	None:intergenic	25.0%
!!	ACAATGTAGATGTTCTATTT+TGG	-	chr2.2:32908830-32908849	MS.gene78200:intron	25.0%
!!	ATTTTTGCCTCATTTTCAAA+TGG	-	chr2.2:32906838-32906857	MS.gene78200:CDS	25.0%
!!	GCTATCAAAAGTATGTTAAA+TGG	+	chr2.2:32909267-32909286	None:intergenic	25.0%
!!	TAACTTTAAAGAGTTACTCT+TGG	-	chr2.2:32907874-32907893	MS.gene78200:CDS	25.0%
!!	TGTTCTTCAATTTTCATTAC+AGG	-	chr2.2:32908218-32908237	MS.gene78200:intron	25.0%
!!!	TCTTATTCTTTGAGATTCAT+AGG	-	chr2.2:32907628-32907647	MS.gene78200:intron	25.0%
	AAATGACTTTACAACAGAGT+GGG	-	chr2.2:32908275-32908294	MS.gene78200:intron	30.0%
	AAATTACTGACCTGAAACTT+GGG	-	chr2.2:32908358-32908377	MS.gene78200:intron	30.0%
	AACCAGCATATTAATCAGTA+AGG	+	chr2.2:32909457-32909476	None:intergenic	30.0%
	AAGAAGAGTTAATACCTAAC+GGG	+	chr2.2:32906896-32906915	None:intergenic	30.0%
	AGTGTAATAGTAGTTGAAGA+AGG	+	chr2.2:32909289-32909308	None:intergenic	30.0%
	ATCTACATTGTCAAATTTGG+AGG	+	chr2.2:32908821-32908840	None:intergenic	30.0%
	ATGGAAGAGACAAAAGTTTA+AGG	+	chr2.2:32909189-32909208	None:intergenic	30.0%
	ATTGAACGACTCCTATAATT+CGG	+	chr2.2:32907560-32907579	None:intergenic	30.0%
	ATTGTCCTATTAATGACTCT+TGG	-	chr2.2:32907950-32907969	MS.gene78200:CDS	30.0%
	CAAGTGAAAAATGTCTTCAT+AGG	-	chr2.2:32907496-32907515	MS.gene78200:CDS	30.0%
	CAGTAATTACAATATAGAGG+AGG	+	chr2.2:32907912-32907931	None:intergenic	30.0%
	GAAGAAGAGTTAATACCTAA+CGG	+	chr2.2:32906897-32906916	None:intergenic	30.0%
	GCGACTAAATTACATATTGA+TGG	+	chr2.2:32907987-32908006	None:intergenic	30.0%
	GTTTGTGAATATCAAAAGAG+AGG	+	chr2.2:32908391-32908410	None:intergenic	30.0%
	TAAATGACTTTACAACAGAG+TGG	-	chr2.2:32908274-32908293	MS.gene78200:intron	30.0%
	TAAATTTCTGACCTGTACAA+TGG	+	chr2.2:32907387-32907406	None:intergenic	30.0%
	TCTGTTATCTTATTGACACT+TGG	-	chr2.2:32908306-32908325	MS.gene78200:intron	30.0%
	TCTTCATGAATGATTCGTAA+AGG	-	chr2.2:32907527-32907546	MS.gene78200:CDS	30.0%
	TGAAATCAAGGGATATGATT+TGG	-	chr2.2:32907849-32907868	MS.gene78200:intron	30.0%
	TGGAAGAGACAAAAGTTTAA+GGG	+	chr2.2:32909188-32909207	None:intergenic	30.0%
	TTTAATGATGCCAACTATGT+CGG	-	chr2.2:32908459-32908478	MS.gene78200:intron	30.0%
!	CAATTTTCATTACAGGCTAT+TGG	-	chr2.2:32908225-32908244	MS.gene78200:intron	30.0%
!	GATGTTAAACCTTTTGCATT+TGG	-	chr2.2:32909052-32909071	MS.gene78200:CDS	30.0%
!	TATGCTGGTTAAAGAACTAT+TGG	-	chr2.2:32909467-32909486	MS.gene78200:CDS	30.0%
!	TTCCTTACTGATTAATATGC+TGG	-	chr2.2:32909452-32909471	MS.gene78200:CDS	30.0%
!	TTCTTGGAAAGTGTAAATGA+GGG	-	chr2.2:32908038-32908057	MS.gene78200:CDS	30.0%
!	TTTCTTGGAAAGTGTAAATG+AGG	-	chr2.2:32908037-32908056	MS.gene78200:CDS	30.0%
!!	ACTCTATGTTTTTGTACTTC+TGG	+	chr2.2:32909720-32909739	None:intergenic	30.0%
!!	ATCCATGCTTTTTAATAGCT+AGG	-	chr2.2:32908131-32908150	MS.gene78200:CDS	30.0%
!!	CAAACAGATTTGATTTTACC+TGG	+	chr2.2:32906789-32906808	None:intergenic	30.0%
!!	CTCTATGTTTTTGTACTTCT+GGG	+	chr2.2:32909719-32909738	None:intergenic	30.0%
!!	GAGGATAGTTCAATTGATTT+TGG	-	chr2.2:32907017-32907036	MS.gene78200:CDS	30.0%
!!!	TTCAACATTTTTGGCAATCT+TGG	-	chr2.2:32909564-32909583	MS.gene78200:intron	30.0%
	AAAGATGAGAGGACCTTAAA+CGG	+	chr2.2:32908707-32908726	None:intergenic	35.0%
	AAGGTTTAACATCGAATGTC+AGG	+	chr2.2:32909045-32909064	None:intergenic	35.0%
	AATCTAGACGCGTAGTTAAT+AGG	+	chr2.2:32908644-32908663	None:intergenic	35.0%
	AGTTGGAATTGTCTATGTTC+AGG	-	chr2.2:32908518-32908537	MS.gene78200:intron	35.0%
	ATATATGCCTTCAACCTCAA+AGG	+	chr2.2:32907587-32907606	None:intergenic	35.0%
	ATTGTTCTGCTTGAAATGTC+TGG	-	chr2.2:32909018-32909037	MS.gene78200:CDS	35.0%
	CAAATTACTGACCTGAAACT+TGG	-	chr2.2:32908357-32908376	MS.gene78200:intron	35.0%
	CAAGATCTTCAGAAAAGAGA+AGG	-	chr2.2:32907305-32907324	MS.gene78200:intron	35.0%
	CATCTTCTTCTTCTTCTACA+TGG	-	chr2.2:32906916-32906935	MS.gene78200:intron	35.0%
	CTATTACACTTTCCTACAAC+TGG	-	chr2.2:32909299-32909318	MS.gene78200:CDS	35.0%
	CTTAGTCGAATATCAGTTCT+GGG	-	chr2.2:32909541-32909560	MS.gene78200:intron	35.0%
	GACCTAGCTATTAAAAAGCA+TGG	+	chr2.2:32908136-32908155	None:intergenic	35.0%
	TAGGGTTTCTTGTATTGTCA+AGG	-	chr2.2:32907084-32907103	MS.gene78200:CDS	35.0%
	TATATGCCTTCAACCTCAAA+GGG	+	chr2.2:32907586-32907605	None:intergenic	35.0%
	TCTTAGTCGAATATCAGTTC+TGG	-	chr2.2:32909540-32909559	MS.gene78200:intron	35.0%
	TGTGCACTAGGAAAACATAT+CGG	-	chr2.2:32908093-32908112	MS.gene78200:CDS	35.0%
	TTTCTTGTATTGTCAAGGCT+TGG	-	chr2.2:32907089-32907108	MS.gene78200:CDS	35.0%
!	AAGGTATCACACCGAATTAT+AGG	-	chr2.2:32907546-32907565	MS.gene78200:intron	35.0%
!	ACAATCTGTGCATTGATGAA+AGG	+	chr2.2:32907251-32907270	None:intergenic	35.0%
!	AGAGGCTTTTCTTGGAAATT+TGG	-	chr2.2:32907142-32907161	MS.gene78200:intron	35.0%
!	GGAAGTTTCTCAAGCTTTTA+GGG	-	chr2.2:32907066-32907085	MS.gene78200:CDS	35.0%
!	GTTGCAATACATTCTGGAAA+TGG	-	chr2.2:32909143-32909162	MS.gene78200:CDS	35.0%
!	TTCTGAAGATCTTGCAACTT+GGG	+	chr2.2:32907299-32907318	None:intergenic	35.0%
!	TTTCTGAAGATCTTGCAACT+TGG	+	chr2.2:32907300-32907319	None:intergenic	35.0%
!!	AAAGGTTGGTAAAAGATGAG+AGG	+	chr2.2:32908718-32908737	None:intergenic	35.0%
!!	GATTTTGGTGACTTCTTCAA+AGG	-	chr2.2:32907032-32907051	MS.gene78200:CDS	35.0%
!!	TGCTTTCAAGTTCTCTTTCT+TGG	-	chr2.2:32908022-32908041	MS.gene78200:CDS	35.0%
!!!	AGTTCTGGGTTCAACATTTT+TGG	-	chr2.2:32909555-32909574	MS.gene78200:intron	35.0%
!!!	TGTAGATGTTCTATTTTGGC+AGG	-	chr2.2:32908834-32908853	MS.gene78200:intron	35.0%
	AAAAGCTTGAGAAACTTCCC+TGG	+	chr2.2:32907065-32907084	None:intergenic	40.0%
	AATTGTTCGCTCACATCATC+TGG	+	chr2.2:32909327-32909346	None:intergenic	40.0%
	AGCGAACAATTGAAACGTCA+AGG	-	chr2.2:32909336-32909355	MS.gene78200:CDS	40.0%
	AGGCATATATGCAGGTTCAT+AGG	-	chr2.2:32907597-32907616	MS.gene78200:intron	40.0%
	ATCTGCTTACCTACCGTTTA+AGG	-	chr2.2:32908691-32908710	MS.gene78200:intron	40.0%
	ATGGAAGAAGATGGTTTGAG+AGG	+	chr2.2:32906710-32906729	None:intergenic	40.0%
	CAGAAAGAAGGACACAATGA+TGG	+	chr2.2:32908493-32908512	None:intergenic	40.0%
	CAGTGCTAGTATGTTTAGGA+AGG	-	chr2.2:32907767-32907786	MS.gene78200:intron	40.0%
	CATTGTGTCCTTCTTTCTGT+TGG	-	chr2.2:32908494-32908513	MS.gene78200:intron	40.0%
	CCAGAATGTATTGCAACAGA+AGG	+	chr2.2:32909140-32909159	None:intergenic	40.0%
	CCCAATGTACGTTGTAATCA+CGG	+	chr2.2:32908334-32908353	None:intergenic	40.0%
	CCGTGATTACAACGTACATT+GGG	-	chr2.2:32908331-32908350	MS.gene78200:intron	40.0%
	CCTTCTGTTGCAATACATTC+TGG	-	chr2.2:32909137-32909156	MS.gene78200:CDS	40.0%
	CGTGATTACAACGTACATTG+GGG	-	chr2.2:32908332-32908351	MS.gene78200:intron	40.0%
	CTTCCTAAACATACTAGCAC+TGG	+	chr2.2:32907769-32907788	None:intergenic	40.0%
	GTTGAGTGGTAGATATTCTC+CGG	+	chr2.2:32906754-32906773	None:intergenic	40.0%
	TACACTCGATATGCTTCTGT+TGG	-	chr2.2:32907356-32907375	MS.gene78200:intron	40.0%
	TATATGCAGGTTCATAGGTG+TGG	-	chr2.2:32907602-32907621	MS.gene78200:intron	40.0%
	TCACCAGTGCTAGTATGTTT+AGG	-	chr2.2:32907763-32907782	MS.gene78200:intron	40.0%
	TCCAACTACCAACAGAAAGA+AGG	+	chr2.2:32908505-32908524	None:intergenic	40.0%
	TCCGTGATTACAACGTACAT+TGG	-	chr2.2:32908330-32908349	MS.gene78200:intron	40.0%
	TCTAGATTCACCAGCAAAAG+CGG	-	chr2.2:32908656-32908675	MS.gene78200:intron	40.0%
	TCTTCAGAAAAGAGAAGGTG+AGG	-	chr2.2:32907310-32907329	MS.gene78200:intron	40.0%
	TGAGAGGACGATTATTAAGC+CGG	+	chr2.2:32906694-32906713	None:intergenic	40.0%
	TGTGATGTAGTGTTAAGTGC+TGG	+	chr2.2:32908158-32908177	None:intergenic	40.0%
	TTGCAACACTGATGATTCTC+AGG	-	chr2.2:32908601-32908620	MS.gene78200:intron	40.0%
	TTTGGCAGGTCAATAGTTCT+GGG	-	chr2.2:32908848-32908867	MS.gene78200:intron	40.0%
!	AGAAGGGGAAGCTTCTTTTA+CGG	+	chr2.2:32906657-32906676	None:intergenic	40.0%
!	CATAGAGCACCAAATGCAAA+AGG	+	chr2.2:32909064-32909083	None:intergenic	40.0%
!	CTTGGTGTGTATATACCTCT+AGG	-	chr2.2:32907107-32907126	MS.gene78200:CDS	40.0%
!	GGGAAGTTTCTCAAGCTTTT+AGG	-	chr2.2:32907065-32907084	MS.gene78200:CDS	40.0%
!	GTTAACAGAGAGGCTTTTCT+TGG	-	chr2.2:32907134-32907153	MS.gene78200:CDS	40.0%
!	TCTTACTTGCCAGCTTCTTT+TGG	-	chr2.2:32908417-32908436	MS.gene78200:intron	40.0%
!	TTTTACCTGGTAGAGAGATG+AGG	+	chr2.2:32906776-32906795	None:intergenic	40.0%
!	TTTTGGCAGGTCAATAGTTC+TGG	-	chr2.2:32908847-32908866	MS.gene78200:intron	40.0%
!!	AATTAGCCCTTTGAGGTTGA+AGG	-	chr2.2:32907577-32907596	MS.gene78200:intron	40.0%
!!	GTATGTGGTTGGTTTATCTC+CGG	-	chr2.2:32908980-32908999	MS.gene78200:intron	40.0%
!!	TATGTGGTTGGTTTATCTCC+GGG	-	chr2.2:32908981-32909000	MS.gene78200:intron	40.0%
!!	TCCTTCTTTCTGTTGGTAGT+TGG	-	chr2.2:32908501-32908520	MS.gene78200:intron	40.0%
	AATGTAGCTGTACTTCTGCC+TGG	+	chr2.2:32909384-32909403	None:intergenic	45.0%
	AATGTCCGACCAAAAGAAGC+TGG	+	chr2.2:32908429-32908448	None:intergenic	45.0%
	ACCAATACCACCACCAGAAA+AGG	+	chr2.2:32907198-32907217	None:intergenic	45.0%
	ACTGACCTGAAACTTGGGAA+TGG	-	chr2.2:32908363-32908382	MS.gene78200:intron	45.0%
	AGACTTGGCATATGCTCTCT+TGG	-	chr2.2:32907218-32907237	MS.gene78200:intron	45.0%
	AGGTTCCATTCCCAAGTTTC+AGG	+	chr2.2:32908371-32908390	None:intergenic	45.0%
	AGTAAGGAAATCAAGGAGCG+TGG	+	chr2.2:32909441-32909460	None:intergenic	45.0%
	AGTCATTTACATAGGGACGC+AGG	+	chr2.2:32908263-32908282	None:intergenic	45.0%
	ATGAGAGGACCTTAAACGGT+AGG	+	chr2.2:32908703-32908722	None:intergenic	45.0%
	CATCATCTGGATCCAGTTGT+AGG	+	chr2.2:32909314-32909333	None:intergenic	45.0%
	GCTCTGCAAATGGAAGAAGA+TGG	+	chr2.2:32906719-32906738	None:intergenic	45.0%
	GGTAACAAGTCCGACATAGT+TGG	+	chr2.2:32908472-32908491	None:intergenic	45.0%
	GGTACTTTAGCACGTTTCAC+TGG	-	chr2.2:32908913-32908932	MS.gene78200:intron	45.0%
	GGTGTGTATATACCTCTAGG+TGG	-	chr2.2:32907110-32907129	MS.gene78200:CDS	45.0%
	GTCGTTCAATTAGCCCTTTG+AGG	-	chr2.2:32907570-32907589	MS.gene78200:intron	45.0%
	TAAACCAACCACATACCTCC+TGG	+	chr2.2:32908976-32908995	None:intergenic	45.0%
	TAGATGTTCCAGCAAATCCC+CGG	+	chr2.2:32909636-32909655	None:intergenic	45.0%
	TCAATTCCACTTGTCAGACC+AGG	-	chr2.2:32909363-32909382	MS.gene78200:CDS	45.0%
	TCTAGGTGGAGTTAACAGAG+AGG	-	chr2.2:32907124-32907143	MS.gene78200:CDS	45.0%
	TCTCTGTTAACTCCACCTAG+AGG	+	chr2.2:32907125-32907144	None:intergenic	45.0%
	TGTTGGATTCGCCATTGTAC+AGG	-	chr2.2:32907373-32907392	MS.gene78200:intron	45.0%
!	ACATCATCGTTAGCTCTTCC+TGG	-	chr2.2:32908892-32908911	MS.gene78200:intron	45.0%
!	ACTCTAGACTCCTTTTCTGG+TGG	-	chr2.2:32907185-32907204	MS.gene78200:intron	45.0%
!	ACTTGCCAGCTTCTTTTGGT+CGG	-	chr2.2:32908421-32908440	MS.gene78200:intron	45.0%
!	AGCACTCTAGACTCCTTTTC+TGG	-	chr2.2:32907182-32907201	MS.gene78200:intron	45.0%
!	CTTTGAATCCAGGAGGTATG+TGG	-	chr2.2:32908965-32908984	MS.gene78200:intron	45.0%
!	GAGGTTGAAGGCATATATGC+AGG	-	chr2.2:32907589-32907608	MS.gene78200:intron	45.0%
!	GTAAGGAAATCAAGGAGCGT+GGG	+	chr2.2:32909440-32909459	None:intergenic	45.0%
!	GTGAAACGTGCTAAAGTACC+AGG	+	chr2.2:32908913-32908932	None:intergenic	45.0%
!	GTTTTCCTAGTGCACAACAG+TGG	+	chr2.2:32908089-32908108	None:intergenic	45.0%
!	TAAGGAAATCAAGGAGCGTG+GGG	+	chr2.2:32909439-32909458	None:intergenic	45.0%
!	TTGGCAGGTCAATAGTTCTG+GGG	-	chr2.2:32908849-32908868	MS.gene78200:intron	45.0%
!!	ACATACTAGCACTGGTGAGA+CGG	+	chr2.2:32907761-32907780	None:intergenic	45.0%
!!	TTTTTGGCAATCTTGGCTGC+TGG	-	chr2.2:32909571-32909590	MS.gene78200:intron	45.0%
!!!	TCCTTTTCTGGTGGTGGTAT+TGG	-	chr2.2:32907194-32907213	MS.gene78200:intron	45.0%
	AAGAAGCTTCCCCTTCTCTC+CGG	-	chr2.2:32906660-32906679	MS.gene78200:CDS	50.0%
	AGATTCACCAGCAAAAGCGG+TGG	-	chr2.2:32908659-32908678	MS.gene78200:intron	50.0%
	AGCTCAGAAAAACCTCGAGG+AGG	-	chr2.2:32906963-32906982	MS.gene78200:intron	50.0%
	AGGAGTAGCAGCTGCAATAG+AGG	-	chr2.2:32906998-32907017	MS.gene78200:CDS	50.0%
	AGGGACGCAGGAAAAGAACT+TGG	+	chr2.2:32908251-32908270	None:intergenic	50.0%
	CAGAAAAGAGAAGGTGAGGC+TGG	-	chr2.2:32907314-32907333	MS.gene78200:intron	50.0%
	CTCAACCACTGTTGTGCACT+AGG	-	chr2.2:32908081-32908100	MS.gene78200:CDS	50.0%
	CTCAACCTCATCTCTCTACC+AGG	-	chr2.2:32906768-32906787	MS.gene78200:CDS	50.0%
	CTGCACACTTGACTGCATTC+CGG	-	chr2.2:32909614-32909633	MS.gene78200:CDS	50.0%
	GAGAGCTCAGAAAAACCTCG+AGG	-	chr2.2:32906960-32906979	MS.gene78200:intron	50.0%
	GCAGTCTGCTCAACAATTGC+AGG	+	chr2.2:32909598-32909617	None:intergenic	50.0%
	GGTAGAGAGATGAGGTTGAG+TGG	+	chr2.2:32906768-32906787	None:intergenic	50.0%
	GTAGCGAATTTCGACCCGTT+AGG	-	chr2.2:32906879-32906898	MS.gene78200:intron	50.0%
	TATTAAGCCGGACACACCAC+CGG	+	chr2.2:32906682-32906701	None:intergenic	50.0%
	TATTCTCCGGACAGAGAGAG+TGG	+	chr2.2:32906741-32906760	None:intergenic	50.0%
	TCAGAAAAACCTCGAGGAGG+AGG	-	chr2.2:32906966-32906985	MS.gene78200:intron	50.0%
	TCTTCAAAGGTCCTCTGCCA+GGG	-	chr2.2:32907045-32907064	MS.gene78200:CDS	50.0%
	TGCACACTTGACTGCATTCC+GGG	-	chr2.2:32909615-32909634	MS.gene78200:CDS	50.0%
	TTCTTCAAAGGTCCTCTGCC+AGG	-	chr2.2:32907044-32907063	MS.gene78200:CDS	50.0%
	TTGAGAAACTTCCCTGGCAG+AGG	+	chr2.2:32907059-32907078	None:intergenic	50.0%
!	AGAGAGAGTGGCTCTGCAAA+TGG	+	chr2.2:32906729-32906748	None:intergenic	50.0%
!	GAATCCAGGAGGTATGTGGT+TGG	-	chr2.2:32908969-32908988	MS.gene78200:intron	50.0%
!!	CTAGACTCCTTTTCTGGTGG+TGG	-	chr2.2:32907188-32907207	MS.gene78200:intron	50.0%
!!	GCAGTAGCTTTGAATCCAGG+AGG	-	chr2.2:32908958-32908977	MS.gene78200:intron	50.0%
!!	GCTGCAGTAGCTTTGAATCC+AGG	-	chr2.2:32908955-32908974	MS.gene78200:intron	50.0%
!!	GGTGGTGGTATTGGTAGACT+TGG	-	chr2.2:32907203-32907222	MS.gene78200:intron	50.0%
!!	TTCAAGTCCACCGCTTTTGC+TGG	+	chr2.2:32908669-32908688	None:intergenic	50.0%
	ACTGCATTCCGGGGATTTGC+TGG	-	chr2.2:32909625-32909644	MS.gene78200:CDS	55.0%
	CTTCTGCCTGGTCTGACAAG+TGG	+	chr2.2:32909372-32909391	None:intergenic	55.0%
	GAAAAACCTCGAGGAGGAGG+AGG	-	chr2.2:32906969-32906988	MS.gene78200:intron	55.0%
	GCACACTTGACTGCATTCCG+GGG	-	chr2.2:32909616-32909635	MS.gene78200:CDS	55.0%
	AAACCTCGAGGAGGAGGAGG+AGG	-	chr2.2:32906972-32906991	MS.gene78200:intron	60.0%
	AAGCTTCCCCTTCTCTCCGG+TGG	-	chr2.2:32906663-32906682	MS.gene78200:CDS	60.0%
	CTTCTCTCCGGTGGTGTGTC+CGG	-	chr2.2:32906672-32906691	MS.gene78200:CDS	60.0%
	GACACACCACCGGAGAGAAG+GGG	+	chr2.2:32906672-32906691	None:intergenic	60.0%
	GCAGAGCCACTCTCTCTGTC+CGG	-	chr2.2:32906732-32906751	MS.gene78200:CDS	60.0%
	GGACACACCACCGGAGAGAA+GGG	+	chr2.2:32906673-32906692	None:intergenic	60.0%
	CGGACACACCACCGGAGAGA+AGG	+	chr2.2:32906674-32906693	None:intergenic	65.0%
	GGAGCGTGGGGCTAGAGAGA+AGG	+	chr2.2:32909427-32909446	None:intergenic	65.0%
	CCTCCTCCTCCTCCTCCTCG+AGG	+	chr2.2:32906978-32906997	None:intergenic	70.0%
	CCTCGAGGAGGAGGAGGAGG+AGG	-	chr2.2:32906975-32906994	MS.gene78200:intron	70.0%
	CGAGGAGGAGGAGGAGGAGG+AGG	-	chr2.2:32906978-32906997	MS.gene78200:intron	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	32906641	32909762	32906641	ID=MS.gene78200
chr2.2	mRNA	32906641	32909762	32906641	ID=MS.gene78200.t1;Parent=MS.gene78200
chr2.2	exon	32909614	32909762	32909614	ID=MS.gene78200.t1.exon1;Parent=MS.gene78200.t1
chr2.2	CDS	32909614	32909762	32909614	ID=cds.MS.gene78200.t1;Parent=MS.gene78200.t1
chr2.2	exon	32909009	32909531	32909009	ID=MS.gene78200.t1.exon2;Parent=MS.gene78200.t1
chr2.2	CDS	32909009	32909531	32909009	ID=cds.MS.gene78200.t1;Parent=MS.gene78200.t1
chr2.2	exon	32907864	32908163	32907864	ID=MS.gene78200.t1.exon3;Parent=MS.gene78200.t1
chr2.2	CDS	32907864	32908163	32907864	ID=cds.MS.gene78200.t1;Parent=MS.gene78200.t1
chr2.2	exon	32907691	32907780	32907691	ID=MS.gene78200.t1.exon4;Parent=MS.gene78200.t1
chr2.2	CDS	32907691	32907780	32907691	ID=cds.MS.gene78200.t1;Parent=MS.gene78200.t1
chr2.2	exon	32907424	32907547	32907424	ID=MS.gene78200.t1.exon5;Parent=MS.gene78200.t1
chr2.2	CDS	32907424	32907547	32907424	ID=cds.MS.gene78200.t1;Parent=MS.gene78200.t1
chr2.2	exon	32906990	32907153	32906990	ID=MS.gene78200.t1.exon6;Parent=MS.gene78200.t1
chr2.2	CDS	32906990	32907153	32906990	ID=cds.MS.gene78200.t1;Parent=MS.gene78200.t1
chr2.2	exon	32906641	32906865	32906641	ID=MS.gene78200.t1.exon7;Parent=MS.gene78200.t1
chr2.2	CDS	32906641	32906865	32906641	ID=cds.MS.gene78200.t1;Parent=MS.gene78200.t1

Gene Sequence

>MS.gene78200

ATGATGTTGATAACCGTAAAAGAAGCTTCCCCTTCTCTCCGGTGGTGTGTCCGGCTTAATAATCGTCCTCTCAAACCATCTTCTTCCATTTGCAGAGCCACTCTCTCTGTCCGGAGAATATCTACCACTCAACCTCATCTCTCTACCAGGTAAAATCAAATCTGTTTGTCTGTATTGTATATTTTTATTCATCATCCATTTTTGCCTCATTTTCAAATGGATTGTATGCAGCTATGACGTAGCGAATTTCGACCCGTTAGGTATTAACTCTTCTTCATCTTCTTCTTCTTCTACATGGAAAAATATTGTGAGTTTGTTTGAGAGCTCAGAAAAACCTCGAGGAGGAGGAGGAGGAGGAGGAGTAGCAGCTGCAATAGAGGATAGTTCAATTGATTTTGGTGACTTCTTCAAAGGTCCTCTGCCAGGGAAGTTTCTCAAGCTTTTAGGGTTTCTTGTATTGTCAAGGCTTGGTGTGTATATACCTCTAGGTGGAGTTAACAGAGAGGCTTTTCTTGGAAATTTGGATCAGAATAGTTTGTTAAGCACTCTAGACTCCTTTTCTGGTGGTGGTATTGGTAGACTTGGCATATGCTCTCTTGGTATTGTTCCTTTCATCAATGCACAGATTGTTTTTCAGCTTCTTGCTCAAGTATATCCCAAGTTGCAAGATCTTCAGAAAAGAGAAGGTGAGGCTGGAAGAAAGAAAATTCTTCAGTACACTCGATATGCTTCTGTTGGATTCGCCATTGTACAGGTCAGAAATTTACATTGCAAACTTTGTTATATAGAATCAATGACATAAATCAAAAGATCTTGTATTGAGTTATTATCCTTTTCTTCATGAATTCAAATAGTCAAGTGAAAAATGTCTTCATAGGTGTCAATGTCTTCATGAATGATTCGTAAAGGTATCACACCGAATTATAGGAGTCGTTCAATTAGCCCTTTGAGGTTGAAGGCATATATGCAGGTTCATAGGTGTGGATCTCTTATTCTTTGAGATTCATAGGTCTCAATACCTTCATGATTTATTCAAAGTTTTTTTGTTGAGATAATGAATGAAAAATTGTTCTTGTTGAGATAATGTTAAAAGGCATACACTATTTAATTGAAATTTCCGTCTCACCAGTGCTAGTATGTTTAGGAAGGAGTAGAAAAAGATTTATGTTAGGGATTAGTAGTGATCAATCATAACCATGAATTAATTTTGAAATCAAGGGATATGATTTGGAGTAACTTTAAAGAGTTACTCTTGGAGTCAATATATCCCTCCTCTATATTGTAATTACTGCATTTAGGGAAACTCTAAATTGTCCTATTAATGACTCTTGGTTATATAATATCCATCAATATGTAATTTAGTCGCACAAATTCACTTGTATGCTTTCAAGTTCTCTTTCTTGGAAAGTGTAAATGAGGGAAAACAGCACTTAACTGCAGCTCAACCACTGTTGTGCACTAGGAAAACATATCGGAGTTCGTTTTTGAAGATCCATGCTTTTTAATAGCTAGGTCCAGCACTTAACACTACATCACACTTATTACTAATTGTCGTTTCCAGTTTATAATTTTGTTTTTGTTCTTCAATTTTCATTACAGGCTATTGGCCAAGTTCTTTTCCTGCGTCCCTATGTAAATGACTTTACAACAGAGTGGGCTCTTACTTCTGTTATCTTATTGACACTTGGCTCCGTGATTACAACGTACATTGGGGAGCAAATTACTGACCTGAAACTTGGGAATGGAACCTCTCTTTTGATATTCACAAACATTATCTCTTACTTGCCAGCTTCTTTTGGTCGGACATTTGCACAAGCATTTAATGATGCCAACTATGTCGGACTTGTTACCATCATTGTGTCCTTCTTTCTGTTGGTAGTTGGAATTGTCTATGTTCAGGTAAATCTCATTTCAATTGTTGAATAATAAAGAAGTATTTGAACAAACTGAGAGATGTTGATTGCAACACTGATGATTCTCAGGAAGCAGAGAGAAAAATTCCTATTAACTACGCGTCTAGATTCACCAGCAAAAGCGGTGGACTTGAAAAATCTGCTTACCTACCGTTTAAGGTCCTCTCATCTTTTACCAACCTTTTTTATGTTACTGTAGTACCTTACTCGAATAGTATTTGATAAACTTTTAATTATGCATATGATAGAGTACAATACAATAAACCTCCAAATTTGACAATGTAGATGTTCTATTTTGGCAGGTCAATAGTTCTGGGGTAATGCCTATCATATTTTCTACATCATCGTTAGCTCTTCCTGGTACTTTAGCACGTTTCACTGGTTTAAGTGCACTAAAAAATGCTGCAGTAGCTTTGAATCCAGGAGGTATGTGGTTGGTTTATCTCCGGGTTTATAATAATAAAATTGTTCTGCTTGAAATGTCTGGTCCTGACATTCGATGTTAAACCTTTTGCATTTGGTGCTCTATGAAACTGTGATTTTAAGAAAAGAAAATGCTGAGAAATGTTTGATGAAATATAGGCCTTCTGTTGCAATACATTCTGGAAATGGAATCTTACATTCTTTGTCGCCCTTAAACTTTTGTCTCTTCCATAAATTGATTCCCTTTACTTAAATGTACATTTTTTTTTGTAGGCTCCTTCTATCTTCCATTTAACATACTTTTGATAGCCTTCTTCAACTACTATTACACTTTCCTACAACTGGATCCAGATGATGTGAGCGAACAATTGAAACGTCAAGGTGCTTCAATTCCACTTGTCAGACCAGGCAGAAGTACAGCTACATTTATTAAAACAGTAATTTTGTCCTTCTCTCTAGCCCCACGCTCCTTGATTTCCTTACTGATTAATATGCTGGTTAAAGAACTATTGGATCCATTCATTTTACATCACTTAATATAAAATATAATATATGGTGCAGGTTCTTAGTCGAATATCAGTTCTGGGTTCAACATTTTTGGCAATCTTGGCTGCTGGACCTGCAATTGTTGAGCAGACTGCACACTTGACTGCATTCCGGGGATTTGCTGGAACATCTATTCTTATTCTTGTTGGTTGTGCAACAGACACTGCTAGAAAAGTTCGAGCTGAGATTATATCCCAGAAGTACAAAAACATAGAGTTATATGACTTTGACAAGTATTGA

Protein sequence

>MS.gene78200.t1

MMLITVKEASPSLRWCVRLNNRPLKPSSSICRATLSVRRISTTQPHLSTSYDVANFDPLGINSSSSSSSSTWKNIVSLFESSEKPRGGGGGGGVAAAIEDSSIDFGDFFKGPLPGKFLKLLGFLVLSRLGVYIPLGGVNREAFLGNLDQNSLLSTLDSFSGGGIGRLGICSLGIVPFINAQIVFQLLAQVYPKLQDLQKREGEAGRKKILQYTRYASVGFAIVQAIGQVLFLRPYVNDFTTEWALTSVILLTLGSVITTYIGEQITDLKLGNGTSLLIFTNIISYLPASFGRTFAQAFNDANYVGLVTIIVSFFLLVVGIVYVQEAERKIPINYASRFTSKSGGLEKSAYLPFKVNSSGVMPIIFSTSSLALPGTLARFTGLSALKNAAVALNPGGSFYLPFNILLIAFFNYYYTFLQLDPDDVSEQLKRQGASIPLVRPGRSTATFIKTVLSRISVLGSTFLAILAAGPAIVEQTAHLTAFRGFAGTSILILVGCATDTARKVRAEIISQKYKNIELYDFDKY