AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0076900

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0076900 MtrunA17_Chr1g0186121 93.488 215 14 0 1 215 98 312 9.93e-143 400
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 65 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGCAAGAGCATGTCTATT+TGG 0.260332 1_2:-53268236 None:intergenic
TACCTGTTATTGAAGACTTT+GGG 0.278202 1_2:+53266952 Msa0076900:CDS
TAGGTTCCATCTCAAGAAAA+AGG 0.324911 1_2:+53268446 Msa0076900:three_prime_UTR
TTCTGCAGAACCGGAGGAAT+TGG 0.348481 1_2:-53266991 None:intergenic
TACCTGTCACTGAAGACTTT+GGG 0.350219 1_2:+53267078 Msa0076900:CDS
TGATCATGTAGGAAAGAAAA+AGG 0.353754 1_2:+53268340 Msa0076900:CDS
AGACTTTGGGGACGGCGGTT+TGG 0.358797 1_2:+53267091 Msa0076900:CDS
CACAAATAAACATTGAAAAC+TGG 0.373736 1_2:-53268529 None:intergenic
ACAGCACGAGGGCGTGGAAT+TGG 0.374576 1_2:-53267212 None:intergenic
TCTTGCGTCCGGAGGAATAT+CGG 0.385319 1_2:-53268285 None:intergenic
CTTCTCTGGTTCTGCAGAAC+CGG 0.386674 1_2:-53267000 None:intergenic
ACAGAAAATGACTTAATGAT+TGG 0.389455 1_2:+53268155 Msa0076900:intron
TCCCCAAAGTCTTCAATAAC+AGG 0.399542 1_2:-53266954 None:intergenic
GTACCTGTTATTGAAGACTT+TGG 0.416459 1_2:+53266951 Msa0076900:CDS
ATGTGACATGATCTTAATCT+AGG 0.417453 1_2:+53268421 Msa0076900:three_prime_UTR
AGCAGAAACAAGACTGGAAA+TGG 0.419403 1_2:+53268179 Msa0076900:CDS
GCATCCGATTTGTCAGGACC+TGG 0.425980 1_2:+53267113 Msa0076900:CDS
TCTATATGGCCGTCCTTCTC+AGG 0.446794 1_2:-53267158 None:intergenic
TCAGTATCTCCATCCTTCTC+TGG 0.448833 1_2:-53266888 None:intergenic
TCAGTATCTCCATCCTTCTC+TGG 0.448833 1_2:-53267014 None:intergenic
TTCTGTAGAACCAGAGGAAT+CGG 0.461876 1_2:-53266865 None:intergenic
ATCAACTTTATCTTTAGACT+TGG 0.466112 1_2:-53268321 None:intergenic
GTACCTGTCACTGAAGACTT+TGG 0.473223 1_2:+53267077 Msa0076900:CDS
CAGAAAATGACTTAATGATT+GGG 0.477294 1_2:+53268156 Msa0076900:intron
CTGATAATTTACATAGGCAC+TGG 0.480813 1_2:+53268384 Msa0076900:CDS
TCCCCAAAGTCTTCAGTGAC+AGG 0.504033 1_2:-53267080 None:intergenic
ATCTTTAGACTTGGGCTCTG+TGG 0.509252 1_2:-53268312 None:intergenic
CATCCGATTTGTCAGGACCT+GGG 0.510606 1_2:+53267114 Msa0076900:CDS
GGTTTGGCATCCGATTTGTC+AGG 0.511838 1_2:+53267107 Msa0076900:CDS
GATATGACAGCACGAGGGCG+TGG 0.515028 1_2:-53267218 None:intergenic
GGTTTGACATCCAATTCCTC+CGG 0.524831 1_2:+53266981 Msa0076900:CDS
AGGTACTCGTGGAATCAAAT+CGG 0.525694 1_2:-53266934 None:intergenic
TCAACTTTATCTTTAGACTT+GGG 0.525773 1_2:-53268320 None:intergenic
ATCCGATTTGTCAGGACCTG+GGG 0.530457 1_2:+53267115 Msa0076900:CDS
CATGATCTTAATCTAGGACT+AGG 0.540490 1_2:+53268427 Msa0076900:three_prime_UTR
CGTGCTGTCATATCTAGTCC+TGG 0.542686 1_2:+53267227 Msa0076900:CDS
AAAGATAAAGTTGATCATGT+AGG 0.545329 1_2:+53268329 Msa0076900:CDS
CGGTTCTGCAGAACCAGAGA+AGG 0.550317 1_2:+53267001 Msa0076900:CDS
AGGAAGATATATAATGCCTC+AGG 0.557283 1_2:-53268584 None:intergenic
ACCTGTTATTGAAGACTTTG+GGG 0.566193 1_2:+53266953 Msa0076900:CDS
CTCTGGTTCTGCAGAACCGG+AGG 0.570758 1_2:-53266997 None:intergenic
ATTGGGAGCAGAAACAAGAC+TGG 0.572746 1_2:+53268173 Msa0076900:CDS
GTTATTGAAGACTTTGGGGA+AGG 0.576122 1_2:+53266957 Msa0076900:CDS
AAGTATTAAGCACGTCGTCA+TGG 0.577628 1_2:-53268501 None:intergenic
CTCTGGTTCTGTAGAACCAG+AGG 0.585302 1_2:-53266871 None:intergenic
TGGTTCTACAGAACCAGAGA+AGG 0.590844 1_2:+53266875 Msa0076900:CDS
ACCTGTCACTGAAGACTTTG+GGG 0.594909 1_2:+53267079 Msa0076900:CDS
TAAAATCTGATAATTTACAT+AGG 0.595012 1_2:+53268378 Msa0076900:CDS
GAAGGATCATGGACCTGAGA+AGG 0.596092 1_2:+53267145 Msa0076900:CDS
TCTTCAATAACAGGTACTCG+TGG 0.602537 1_2:-53266945 None:intergenic
TGACACTACCGATATTCCTC+CGG 0.603823 1_2:+53268277 Msa0076900:CDS
ATTGAAGACTTTGGGGAAGG+CGG 0.604745 1_2:+53266960 Msa0076900:CDS
TCTGCAGAACCAGAGAAGGA+TGG 0.614650 1_2:+53267005 Msa0076900:CDS
TCTACAGAACCAGAGAAGGA+TGG 0.618996 1_2:+53266879 Msa0076900:CDS
AGATTAAGATCATGTCACAT+TGG 0.629085 1_2:-53268418 None:intergenic
ACTGAAGACTTTGGGGACGG+CGG 0.636584 1_2:+53267086 Msa0076900:CDS
AAATCTTAAGCAAAAGAGAG+AGG 0.638186 1_2:-53268604 None:intergenic
TCTTCAGTGACAGGTACTCG+TGG 0.639277 1_2:-53267071 None:intergenic
GATCATGGACCTGAGAAGGA+CGG 0.642589 1_2:+53267149 Msa0076900:CDS
TGTAAGATTATTAAGCAACA+AGG 0.649360 1_2:+53268556 Msa0076900:three_prime_UTR
GTCACTGAAGACTTTGGGGA+CGG 0.652392 1_2:+53267083 Msa0076900:CDS
TCCGATTTGTCAGGACCTGG+GGG 0.661422 1_2:+53267116 Msa0076900:CDS
GGACTAGATATGACAGCACG+AGG 0.661674 1_2:-53267224 None:intergenic
AAGCAACAAGGCATTGCCTG+AGG 0.670667 1_2:+53268568 Msa0076900:three_prime_UTR
GACTAGATATGACAGCACGA+GGG 0.734110 1_2:-53267223 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAGTAATATTATATTGTT+AGG + chr1_2:53267732-53267751 Msa0076900:intron 10.0%
!! TTAAGTAATATTATATTGTT+AGG + chr1_2:53267732-53267751 Msa0076900:intron 10.0%
!! TAAAATCTGATAATTTACAT+AGG + chr1_2:53268378-53268397 Msa0076900:CDS 15.0%
!! TAAAATCTGATAATTTACAT+AGG + chr1_2:53268378-53268397 Msa0076900:CDS 15.0%
!! ATAAAATTGCAAAAAGTAAC+TGG - chr1_2:53267310-53267329 None:intergenic 20.0%
!!! TATCATAGATAACAATGTTT+TGG + chr1_2:53267391-53267410 Msa0076900:intron 20.0%
!!! ATCATAGATAACAATGTTTT+GGG + chr1_2:53267392-53267411 Msa0076900:intron 20.0%
!! AATATTTGTGTATGTTCTAA+AGG - chr1_2:53267468-53267487 None:intergenic 20.0%
!!! GTACTGAAAAGAATATTTTA+GGG + chr1_2:53267667-53267686 Msa0076900:intron 20.0%
!! ATAAAATTGCAAAAAGTAAC+TGG - chr1_2:53267310-53267329 None:intergenic 20.0%
!!! TATCATAGATAACAATGTTT+TGG + chr1_2:53267391-53267410 Msa0076900:intron 20.0%
!!! ATCATAGATAACAATGTTTT+GGG + chr1_2:53267392-53267411 Msa0076900:intron 20.0%
!! AATATTTGTGTATGTTCTAA+AGG - chr1_2:53267468-53267487 None:intergenic 20.0%
!!! GTACTGAAAAGAATATTTTA+GGG + chr1_2:53267667-53267686 Msa0076900:intron 20.0%
!!! CTTTTTTCTCATCATCTATA+TGG - chr1_2:53267175-53267194 None:intergenic 25.0%
! ATAAAGCATAACTGTTCTTA+TGG - chr1_2:53267372-53267391 None:intergenic 25.0%
! AAGGTAAACAAAACTCATAA+TGG + chr1_2:53267582-53267601 Msa0076900:intron 25.0%
!!! GGTACTGAAAAGAATATTTT+AGG + chr1_2:53267666-53267685 Msa0076900:intron 25.0%
!! AGGTTTATTATGTAGCTTTT+CGG + chr1_2:53268108-53268127 Msa0076900:intron 25.0%
! ACAGAAAATGACTTAATGAT+TGG + chr1_2:53268155-53268174 Msa0076900:intron 25.0%
! CAGAAAATGACTTAATGATT+GGG + chr1_2:53268156-53268175 Msa0076900:intron 25.0%
! TCAACTTTATCTTTAGACTT+GGG - chr1_2:53268323-53268342 None:intergenic 25.0%
! ATCAACTTTATCTTTAGACT+TGG - chr1_2:53268324-53268343 None:intergenic 25.0%
!! AAAGATAAAGTTGATCATGT+AGG + chr1_2:53268329-53268348 Msa0076900:CDS 25.0%
! CACAAATAAACATTGAAAAC+TGG - chr1_2:53268532-53268551 None:intergenic 25.0%
! TGTAAGATTATTAAGCAACA+AGG + chr1_2:53268556-53268575 Msa0076900:three_prime_UTR 25.0%
!!! CTTTTTTCTCATCATCTATA+TGG - chr1_2:53267175-53267194 None:intergenic 25.0%
! ATAAAGCATAACTGTTCTTA+TGG - chr1_2:53267372-53267391 None:intergenic 25.0%
! AAGGTAAACAAAACTCATAA+TGG + chr1_2:53267582-53267601 Msa0076900:intron 25.0%
!!! GGTACTGAAAAGAATATTTT+AGG + chr1_2:53267666-53267685 Msa0076900:intron 25.0%
!! AGGTTTATTATGTAGCTTTT+CGG + chr1_2:53268108-53268127 Msa0076900:intron 25.0%
! ACAGAAAATGACTTAATGAT+TGG + chr1_2:53268155-53268174 Msa0076900:intron 25.0%
! CAGAAAATGACTTAATGATT+GGG + chr1_2:53268156-53268175 Msa0076900:intron 25.0%
! TCAACTTTATCTTTAGACTT+GGG - chr1_2:53268323-53268342 None:intergenic 25.0%
! ATCAACTTTATCTTTAGACT+TGG - chr1_2:53268324-53268343 None:intergenic 25.0%
!! AAAGATAAAGTTGATCATGT+AGG + chr1_2:53268329-53268348 Msa0076900:CDS 25.0%
! CACAAATAAACATTGAAAAC+TGG - chr1_2:53268532-53268551 None:intergenic 25.0%
! TGTAAGATTATTAAGCAACA+AGG + chr1_2:53268556-53268575 Msa0076900:three_prime_UTR 25.0%
TACCTGTTATTGAAGACTTT+GGG + chr1_2:53266952-53266971 Msa0076900:CDS 30.0%
ATAGCTAAACAGAAAACTGA+TGG - chr1_2:53267266-53267285 None:intergenic 30.0%
ATTGTCTGAGCTATAACTAA+AGG + chr1_2:53267328-53267347 Msa0076900:intron 30.0%
TTACCAACATCTATATCTCT+TGG - chr1_2:53267441-53267460 None:intergenic 30.0%
! AAAGTACTTCAAATTTGCTC+TGG - chr1_2:53267561-53267580 None:intergenic 30.0%
! GCAAATTTGAAGTACTTTGA+AGG + chr1_2:53267563-53267582 Msa0076900:intron 30.0%
TATGCATTAAGTGAGAATAC+AGG + chr1_2:53267846-53267865 Msa0076900:intron 30.0%
ATGCATTAAGTGAGAATACA+GGG + chr1_2:53267847-53267866 Msa0076900:intron 30.0%
ATCTCTATAGCAAATAAGCA+AGG + chr1_2:53267879-53267898 Msa0076900:intron 30.0%
!! TAATTTTCACTTCTAGCTGA+TGG + chr1_2:53267946-53267965 Msa0076900:intron 30.0%
AGAATTCAATTCGTTGTTTG+TGG + chr1_2:53267974-53267993 Msa0076900:intron 30.0%
ACTAGAATCAGCATAATTGA+TGG - chr1_2:53268054-53268073 None:intergenic 30.0%
! TCTACTTTCTGTGTTATTCA+AGG + chr1_2:53268088-53268107 Msa0076900:intron 30.0%
TGATCATGTAGGAAAGAAAA+AGG + chr1_2:53268340-53268359 Msa0076900:CDS 30.0%
AGATTAAGATCATGTCACAT+TGG - chr1_2:53268421-53268440 None:intergenic 30.0%
ATGTGACATGATCTTAATCT+AGG + chr1_2:53268421-53268440 Msa0076900:three_prime_UTR 30.0%
AAATCTTAAGCAAAAGAGAG+AGG - chr1_2:53268607-53268626 None:intergenic 30.0%
! TCTCTTTTGCTTAAGATTTC+AGG + chr1_2:53268608-53268627 Msa0076900:three_prime_UTR 30.0%
TACCTGTTATTGAAGACTTT+GGG + chr1_2:53266952-53266971 Msa0076900:CDS 30.0%
ATAGCTAAACAGAAAACTGA+TGG - chr1_2:53267266-53267285 None:intergenic 30.0%
ATTGTCTGAGCTATAACTAA+AGG + chr1_2:53267328-53267347 Msa0076900:intron 30.0%
TTACCAACATCTATATCTCT+TGG - chr1_2:53267441-53267460 None:intergenic 30.0%
! AAAGTACTTCAAATTTGCTC+TGG - chr1_2:53267561-53267580 None:intergenic 30.0%
! GCAAATTTGAAGTACTTTGA+AGG + chr1_2:53267563-53267582 Msa0076900:intron 30.0%
TATGCATTAAGTGAGAATAC+AGG + chr1_2:53267846-53267865 Msa0076900:intron 30.0%
ATGCATTAAGTGAGAATACA+GGG + chr1_2:53267847-53267866 Msa0076900:intron 30.0%
ATCTCTATAGCAAATAAGCA+AGG + chr1_2:53267879-53267898 Msa0076900:intron 30.0%
!! TAATTTTCACTTCTAGCTGA+TGG + chr1_2:53267946-53267965 Msa0076900:intron 30.0%
AGAATTCAATTCGTTGTTTG+TGG + chr1_2:53267974-53267993 Msa0076900:intron 30.0%
ACTAGAATCAGCATAATTGA+TGG - chr1_2:53268054-53268073 None:intergenic 30.0%
! TCTACTTTCTGTGTTATTCA+AGG + chr1_2:53268088-53268107 Msa0076900:intron 30.0%
TGATCATGTAGGAAAGAAAA+AGG + chr1_2:53268340-53268359 Msa0076900:CDS 30.0%
AGATTAAGATCATGTCACAT+TGG - chr1_2:53268421-53268440 None:intergenic 30.0%
ATGTGACATGATCTTAATCT+AGG + chr1_2:53268421-53268440 Msa0076900:three_prime_UTR 30.0%
AAATCTTAAGCAAAAGAGAG+AGG - chr1_2:53268607-53268626 None:intergenic 30.0%
! TCTCTTTTGCTTAAGATTTC+AGG + chr1_2:53268608-53268627 Msa0076900:three_prime_UTR 30.0%
GTACCTGTTATTGAAGACTT+TGG + chr1_2:53266951-53266970 Msa0076900:CDS 35.0%
ACCTGTTATTGAAGACTTTG+GGG + chr1_2:53266953-53266972 Msa0076900:CDS 35.0%
! ACTAAAGGTTGACTTAGAGA+TGG + chr1_2:53267343-53267362 Msa0076900:intron 35.0%
CTATATCTCTTGGCTTCAAT+GGG - chr1_2:53267431-53267450 None:intergenic 35.0%
TCTATATCTCTTGGCTTCAA+TGG - chr1_2:53267432-53267451 None:intergenic 35.0%
AAGCCAAGAGATATAGATGT+TGG + chr1_2:53267435-53267454 Msa0076900:intron 35.0%
!! CATAATGGAAGCACTTCATA+TGG + chr1_2:53267597-53267616 Msa0076900:intron 35.0%
AGTGACATGAAAAGAAACCT+TGG + chr1_2:53267643-53267662 Msa0076900:intron 35.0%
GTGACATGAAAAGAAACCTT+GGG + chr1_2:53267644-53267663 Msa0076900:intron 35.0%
TGACATGAAAAGAAACCTTG+GGG + chr1_2:53267645-53267664 Msa0076900:intron 35.0%
! ATATTCTTTTCAGTACCCCA+AGG - chr1_2:53267663-53267682 None:intergenic 35.0%
! AAACTTGAATTTTGAGCCTG+TGG + chr1_2:53267691-53267710 Msa0076900:intron 35.0%
! TATTGTTAGGAGACTGCTTT+TGG + chr1_2:53267745-53267764 Msa0076900:intron 35.0%
TCTCACTTAATGCATACCAT+AGG - chr1_2:53267842-53267861 None:intergenic 35.0%
GGAAAGAAAAAGGTTCACAA+AGG + chr1_2:53268350-53268369 Msa0076900:CDS 35.0%
CTGATAATTTACATAGGCAC+TGG + chr1_2:53268384-53268403 Msa0076900:CDS 35.0%
! CATGATCTTAATCTAGGACT+AGG + chr1_2:53268427-53268446 Msa0076900:three_prime_UTR 35.0%
TAGGTTCCATCTCAAGAAAA+AGG + chr1_2:53268446-53268465 Msa0076900:three_prime_UTR 35.0%
AGGTTCCATCTCAAGAAAAA+GGG + chr1_2:53268447-53268466 Msa0076900:three_prime_UTR 35.0%
! TCTTTCCCTTTTTCTTGAGA+TGG - chr1_2:53268455-53268474 None:intergenic 35.0%
AGGAAGATATATAATGCCTC+AGG - chr1_2:53268587-53268606 None:intergenic 35.0%
GTACCTGTTATTGAAGACTT+TGG + chr1_2:53266951-53266970 Msa0076900:CDS 35.0%
ACCTGTTATTGAAGACTTTG+GGG + chr1_2:53266953-53266972 Msa0076900:CDS 35.0%
! ACTAAAGGTTGACTTAGAGA+TGG + chr1_2:53267343-53267362 Msa0076900:intron 35.0%
CTATATCTCTTGGCTTCAAT+GGG - chr1_2:53267431-53267450 None:intergenic 35.0%
TCTATATCTCTTGGCTTCAA+TGG - chr1_2:53267432-53267451 None:intergenic 35.0%
AAGCCAAGAGATATAGATGT+TGG + chr1_2:53267435-53267454 Msa0076900:intron 35.0%
!! CATAATGGAAGCACTTCATA+TGG + chr1_2:53267597-53267616 Msa0076900:intron 35.0%
AGTGACATGAAAAGAAACCT+TGG + chr1_2:53267643-53267662 Msa0076900:intron 35.0%
GTGACATGAAAAGAAACCTT+GGG + chr1_2:53267644-53267663 Msa0076900:intron 35.0%
TGACATGAAAAGAAACCTTG+GGG + chr1_2:53267645-53267664 Msa0076900:intron 35.0%
! ATATTCTTTTCAGTACCCCA+AGG - chr1_2:53267663-53267682 None:intergenic 35.0%
! AAACTTGAATTTTGAGCCTG+TGG + chr1_2:53267691-53267710 Msa0076900:intron 35.0%
! TATTGTTAGGAGACTGCTTT+TGG + chr1_2:53267745-53267764 Msa0076900:intron 35.0%
TCTCACTTAATGCATACCAT+AGG - chr1_2:53267842-53267861 None:intergenic 35.0%
GGAAAGAAAAAGGTTCACAA+AGG + chr1_2:53268350-53268369 Msa0076900:CDS 35.0%
CTGATAATTTACATAGGCAC+TGG + chr1_2:53268384-53268403 Msa0076900:CDS 35.0%
! CATGATCTTAATCTAGGACT+AGG + chr1_2:53268427-53268446 Msa0076900:three_prime_UTR 35.0%
TAGGTTCCATCTCAAGAAAA+AGG + chr1_2:53268446-53268465 Msa0076900:three_prime_UTR 35.0%
AGGTTCCATCTCAAGAAAAA+GGG + chr1_2:53268447-53268466 Msa0076900:three_prime_UTR 35.0%
! TCTTTCCCTTTTTCTTGAGA+TGG - chr1_2:53268455-53268474 None:intergenic 35.0%
AGGAAGATATATAATGCCTC+AGG - chr1_2:53268587-53268606 None:intergenic 35.0%
TTCTGTAGAACCAGAGGAAT+CGG - chr1_2:53266868-53266887 None:intergenic 40.0%
AGGTACTCGTGGAATCAAAT+CGG - chr1_2:53266937-53266956 None:intergenic 40.0%
TCTTCAATAACAGGTACTCG+TGG - chr1_2:53266948-53266967 None:intergenic 40.0%
TCCCCAAAGTCTTCAATAAC+AGG - chr1_2:53266957-53266976 None:intergenic 40.0%
! GTTATTGAAGACTTTGGGGA+AGG + chr1_2:53266957-53266976 Msa0076900:CDS 40.0%
TACCTGTCACTGAAGACTTT+GGG + chr1_2:53267078-53267097 Msa0076900:CDS 40.0%
GATGGAGTATGAGATTTACC+AGG - chr1_2:53267248-53267267 None:intergenic 40.0%
AGAGTTGCATTGCATCATTC+TGG - chr1_2:53267514-53267533 None:intergenic 40.0%
TTAATGCATACCATAGGCTC+TGG - chr1_2:53267836-53267855 None:intergenic 40.0%
GTTTGTGGTTGTGAAGAGAA+TGG + chr1_2:53267989-53268008 Msa0076900:intron 40.0%
CAGAAAGTAGATGCAAAGTG+AGG - chr1_2:53268079-53268098 None:intergenic 40.0%
AGCAGAAACAAGACTGGAAA+TGG + chr1_2:53268179-53268198 Msa0076900:CDS 40.0%
! TGAGCAAGAGCATGTCTATT+TGG - chr1_2:53268239-53268258 None:intergenic 40.0%
AAGTATTAAGCACGTCGTCA+TGG - chr1_2:53268504-53268523 None:intergenic 40.0%
TTCTGTAGAACCAGAGGAAT+CGG - chr1_2:53266868-53266887 None:intergenic 40.0%
AGGTACTCGTGGAATCAAAT+CGG - chr1_2:53266937-53266956 None:intergenic 40.0%
TCTTCAATAACAGGTACTCG+TGG - chr1_2:53266948-53266967 None:intergenic 40.0%
TCCCCAAAGTCTTCAATAAC+AGG - chr1_2:53266957-53266976 None:intergenic 40.0%
! GTTATTGAAGACTTTGGGGA+AGG + chr1_2:53266957-53266976 Msa0076900:CDS 40.0%
TACCTGTCACTGAAGACTTT+GGG + chr1_2:53267078-53267097 Msa0076900:CDS 40.0%
GATGGAGTATGAGATTTACC+AGG - chr1_2:53267248-53267267 None:intergenic 40.0%
AGAGTTGCATTGCATCATTC+TGG - chr1_2:53267514-53267533 None:intergenic 40.0%
TTAATGCATACCATAGGCTC+TGG - chr1_2:53267836-53267855 None:intergenic 40.0%
GTTTGTGGTTGTGAAGAGAA+TGG + chr1_2:53267989-53268008 Msa0076900:intron 40.0%
CAGAAAGTAGATGCAAAGTG+AGG - chr1_2:53268079-53268098 None:intergenic 40.0%
AGCAGAAACAAGACTGGAAA+TGG + chr1_2:53268179-53268198 Msa0076900:CDS 40.0%
! TGAGCAAGAGCATGTCTATT+TGG - chr1_2:53268239-53268258 None:intergenic 40.0%
AAGTATTAAGCACGTCGTCA+TGG - chr1_2:53268504-53268523 None:intergenic 40.0%
! TGGTTCTACAGAACCAGAGA+AGG + chr1_2:53266875-53266894 Msa0076900:CDS 45.0%
TCTACAGAACCAGAGAAGGA+TGG + chr1_2:53266879-53266898 Msa0076900:CDS 45.0%
TCAGTATCTCCATCCTTCTC+TGG - chr1_2:53266891-53266910 None:intergenic 45.0%
! ATTGAAGACTTTGGGGAAGG+CGG + chr1_2:53266960-53266979 Msa0076900:CDS 45.0%
GGTTTGACATCCAATTCCTC+CGG + chr1_2:53266981-53267000 Msa0076900:CDS 45.0%
TCAGTATCTCCATCCTTCTC+TGG - chr1_2:53266891-53266910 None:intergenic 45.0%
GTACCTGTCACTGAAGACTT+TGG + chr1_2:53267077-53267096 Msa0076900:CDS 45.0%
ACCTGTCACTGAAGACTTTG+GGG + chr1_2:53267079-53267098 Msa0076900:CDS 45.0%
GACTAGATATGACAGCACGA+GGG - chr1_2:53267226-53267245 None:intergenic 45.0%
! GAATTTTGAGCCTGTGGTTG+TGG + chr1_2:53267697-53267716 Msa0076900:intron 45.0%
GATGTAAATGCCACAACCAC+AGG - chr1_2:53267710-53267729 None:intergenic 45.0%
! TCATTTTGCTCCAGAGCCTA+TGG + chr1_2:53267823-53267842 Msa0076900:intron 45.0%
ATTGGGAGCAGAAACAAGAC+TGG + chr1_2:53268173-53268192 Msa0076900:CDS 45.0%
TGACACTACCGATATTCCTC+CGG + chr1_2:53268277-53268296 Msa0076900:CDS 45.0%
! TCTGTGGATTTTCTTGCGTC+CGG - chr1_2:53268299-53268318 None:intergenic 45.0%
ATCTTTAGACTTGGGCTCTG+TGG - chr1_2:53268315-53268334 None:intergenic 45.0%
! TGGTTCTACAGAACCAGAGA+AGG + chr1_2:53266875-53266894 Msa0076900:CDS 45.0%
TCTACAGAACCAGAGAAGGA+TGG + chr1_2:53266879-53266898 Msa0076900:CDS 45.0%
TCAGTATCTCCATCCTTCTC+TGG - chr1_2:53266891-53266910 None:intergenic 45.0%
! ATTGAAGACTTTGGGGAAGG+CGG + chr1_2:53266960-53266979 Msa0076900:CDS 45.0%
GGTTTGACATCCAATTCCTC+CGG + chr1_2:53266981-53267000 Msa0076900:CDS 45.0%
TCAGTATCTCCATCCTTCTC+TGG - chr1_2:53266891-53266910 None:intergenic 45.0%
GTACCTGTCACTGAAGACTT+TGG + chr1_2:53267077-53267096 Msa0076900:CDS 45.0%
ACCTGTCACTGAAGACTTTG+GGG + chr1_2:53267079-53267098 Msa0076900:CDS 45.0%
GACTAGATATGACAGCACGA+GGG - chr1_2:53267226-53267245 None:intergenic 45.0%
! GAATTTTGAGCCTGTGGTTG+TGG + chr1_2:53267697-53267716 Msa0076900:intron 45.0%
GATGTAAATGCCACAACCAC+AGG - chr1_2:53267710-53267729 None:intergenic 45.0%
! TCATTTTGCTCCAGAGCCTA+TGG + chr1_2:53267823-53267842 Msa0076900:intron 45.0%
ATTGGGAGCAGAAACAAGAC+TGG + chr1_2:53268173-53268192 Msa0076900:CDS 45.0%
TGACACTACCGATATTCCTC+CGG + chr1_2:53268277-53268296 Msa0076900:CDS 45.0%
! TCTGTGGATTTTCTTGCGTC+CGG - chr1_2:53268299-53268318 None:intergenic 45.0%
ATCTTTAGACTTGGGCTCTG+TGG - chr1_2:53268315-53268334 None:intergenic 45.0%
!! CTCTGGTTCTGTAGAACCAG+AGG - chr1_2:53266874-53266893 None:intergenic 50.0%
TTCTGCAGAACCGGAGGAAT+TGG - chr1_2:53266994-53267013 None:intergenic 50.0%
!! CTTCTCTGGTTCTGCAGAAC+CGG - chr1_2:53267003-53267022 None:intergenic 50.0%
TCTGCAGAACCAGAGAAGGA+TGG + chr1_2:53267005-53267024 Msa0076900:CDS 50.0%
TCTTCAGTGACAGGTACTCG+TGG - chr1_2:53267074-53267093 None:intergenic 50.0%
TCCCCAAAGTCTTCAGTGAC+AGG - chr1_2:53267083-53267102 None:intergenic 50.0%
! GTCACTGAAGACTTTGGGGA+CGG + chr1_2:53267083-53267102 Msa0076900:CDS 50.0%
! GGTTTGGCATCCGATTTGTC+AGG + chr1_2:53267107-53267126 Msa0076900:CDS 50.0%
! CATCCGATTTGTCAGGACCT+GGG + chr1_2:53267114-53267133 Msa0076900:CDS 50.0%
! ATCCGATTTGTCAGGACCTG+GGG + chr1_2:53267115-53267134 Msa0076900:CDS 50.0%
GAAGGATCATGGACCTGAGA+AGG + chr1_2:53267145-53267164 Msa0076900:CDS 50.0%
GATCATGGACCTGAGAAGGA+CGG + chr1_2:53267149-53267168 Msa0076900:CDS 50.0%
TCTATATGGCCGTCCTTCTC+AGG - chr1_2:53267161-53267180 None:intergenic 50.0%
GGACTAGATATGACAGCACG+AGG - chr1_2:53267227-53267246 None:intergenic 50.0%
CGTGCTGTCATATCTAGTCC+TGG + chr1_2:53267227-53267246 Msa0076900:CDS 50.0%
TCTTGCGTCCGGAGGAATAT+CGG - chr1_2:53268288-53268307 None:intergenic 50.0%
!! AAGCAACAAGGCATTGCCTG+AGG + chr1_2:53268568-53268587 Msa0076900:three_prime_UTR 50.0%
!! CTCTGGTTCTGTAGAACCAG+AGG - chr1_2:53266874-53266893 None:intergenic 50.0%
TTCTGCAGAACCGGAGGAAT+TGG - chr1_2:53266994-53267013 None:intergenic 50.0%
!! CTTCTCTGGTTCTGCAGAAC+CGG - chr1_2:53267003-53267022 None:intergenic 50.0%
TCTGCAGAACCAGAGAAGGA+TGG + chr1_2:53267005-53267024 Msa0076900:CDS 50.0%
TCTTCAGTGACAGGTACTCG+TGG - chr1_2:53267074-53267093 None:intergenic 50.0%
TCCCCAAAGTCTTCAGTGAC+AGG - chr1_2:53267083-53267102 None:intergenic 50.0%
! GTCACTGAAGACTTTGGGGA+CGG + chr1_2:53267083-53267102 Msa0076900:CDS 50.0%
! GGTTTGGCATCCGATTTGTC+AGG + chr1_2:53267107-53267126 Msa0076900:CDS 50.0%
! CATCCGATTTGTCAGGACCT+GGG + chr1_2:53267114-53267133 Msa0076900:CDS 50.0%
! ATCCGATTTGTCAGGACCTG+GGG + chr1_2:53267115-53267134 Msa0076900:CDS 50.0%
GAAGGATCATGGACCTGAGA+AGG + chr1_2:53267145-53267164 Msa0076900:CDS 50.0%
GATCATGGACCTGAGAAGGA+CGG + chr1_2:53267149-53267168 Msa0076900:CDS 50.0%
TCTATATGGCCGTCCTTCTC+AGG - chr1_2:53267161-53267180 None:intergenic 50.0%
GGACTAGATATGACAGCACG+AGG - chr1_2:53267227-53267246 None:intergenic 50.0%
CGTGCTGTCATATCTAGTCC+TGG + chr1_2:53267227-53267246 Msa0076900:CDS 50.0%
TCTTGCGTCCGGAGGAATAT+CGG - chr1_2:53268288-53268307 None:intergenic 50.0%
!! AAGCAACAAGGCATTGCCTG+AGG + chr1_2:53268568-53268587 Msa0076900:three_prime_UTR 50.0%
CGGTTCTGCAGAACCAGAGA+AGG + chr1_2:53267001-53267020 Msa0076900:CDS 55.0%
! ACTGAAGACTTTGGGGACGG+CGG + chr1_2:53267086-53267105 Msa0076900:CDS 55.0%
! GCATCCGATTTGTCAGGACC+TGG + chr1_2:53267113-53267132 Msa0076900:CDS 55.0%
! TCCGATTTGTCAGGACCTGG+GGG + chr1_2:53267116-53267135 Msa0076900:CDS 55.0%
TCCCCCAGGTCCTGACAAAT+CGG - chr1_2:53267120-53267139 None:intergenic 55.0%
TGATCCTTCTCTAGTCCCCC+AGG - chr1_2:53267134-53267153 None:intergenic 55.0%
GGGGGACTAGAGAAGGATCA+TGG + chr1_2:53267134-53267153 Msa0076900:CDS 55.0%
! GTGGATTTTCTTGCGTCCGG+AGG - chr1_2:53268296-53268315 None:intergenic 55.0%
CGGTTCTGCAGAACCAGAGA+AGG + chr1_2:53267001-53267020 Msa0076900:CDS 55.0%
! ACTGAAGACTTTGGGGACGG+CGG + chr1_2:53267086-53267105 Msa0076900:CDS 55.0%
! GCATCCGATTTGTCAGGACC+TGG + chr1_2:53267113-53267132 Msa0076900:CDS 55.0%
! TCCGATTTGTCAGGACCTGG+GGG + chr1_2:53267116-53267135 Msa0076900:CDS 55.0%
TCCCCCAGGTCCTGACAAAT+CGG - chr1_2:53267120-53267139 None:intergenic 55.0%
TGATCCTTCTCTAGTCCCCC+AGG - chr1_2:53267134-53267153 None:intergenic 55.0%
GGGGGACTAGAGAAGGATCA+TGG + chr1_2:53267134-53267153 Msa0076900:CDS 55.0%
! GTGGATTTTCTTGCGTCCGG+AGG - chr1_2:53268296-53268315 None:intergenic 55.0%
!! CTCTGGTTCTGCAGAACCGG+AGG - chr1_2:53267000-53267019 None:intergenic 60.0%
! AGACTTTGGGGACGGCGGTT+TGG + chr1_2:53267091-53267110 Msa0076900:CDS 60.0%
AGGACCTGGGGGACTAGAGA+AGG + chr1_2:53267127-53267146 Msa0076900:CDS 60.0%
! ACAGCACGAGGGCGTGGAAT+TGG - chr1_2:53267215-53267234 None:intergenic 60.0%
GATATGACAGCACGAGGGCG+TGG - chr1_2:53267221-53267240 None:intergenic 60.0%
!! CTCTGGTTCTGCAGAACCGG+AGG - chr1_2:53267000-53267019 None:intergenic 60.0%
! AGACTTTGGGGACGGCGGTT+TGG + chr1_2:53267091-53267110 Msa0076900:CDS 60.0%
AGGACCTGGGGGACTAGAGA+AGG + chr1_2:53267127-53267146 Msa0076900:CDS 60.0%
! ACAGCACGAGGGCGTGGAAT+TGG - chr1_2:53267215-53267234 None:intergenic 60.0%
GATATGACAGCACGAGGGCG+TGG - chr1_2:53267221-53267240 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 53266858 53268689 53266858 ID=Msa0076900;Name=Msa0076900
chr1_2 mRNA 53266858 53268689 53266858 ID=Msa0076900-mRNA-1;Parent=Msa0076900;Name=Msa0076900-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|1|1|1|1|1|2|274|215
chr1_2 exon 53266858 53267248 53266858 ID=Msa0076900-mRNA-1:exon:16459;Parent=Msa0076900-mRNA-1
chr1_2 exon 53268159 53268689 53268159 ID=Msa0076900-mRNA-1:exon:16460;Parent=Msa0076900-mRNA-1
chr1_2 CDS 53266858 53267248 53266858 ID=Msa0076900-mRNA-1:cds;Parent=Msa0076900-mRNA-1
chr1_2 CDS 53268159 53268415 53268159 ID=Msa0076900-mRNA-1:cds;Parent=Msa0076900-mRNA-1
chr1_2 three_prime_UTR 53268416 53268689 53268416 ID=Msa0076900-mRNA-1:three_prime_utr;Parent=Msa0076900-mRNA-1
Gene Sequence

>Msa0076900

TTGACATCCGATTCCTCTGGTTCTACAGAACCAGAGAAGGATGGAGATACTGATGAGAGTAAAGTATATACAAGAGCCGATTTGATTCCACGAGTACCTGTTATTGAAGACTTTGGGGAAGGCGGTTTGACATCCAATTCCTCCGGTTCTGCAGAACCAGAGAAGGATGGAGATACTGATGAGAGTAAAGTATATATTAGAGTCGATTTGATTCCACGAGTACCTGTCACTGAAGACTTTGGGGACGGCGGTTTGGCATCCGATTTGTCAGGACCTGGGGGACTAGAGAAGGATCATGGACCTGAGAAGGACGGCCATATAGATGATGAGAAAAAAGTAAACATCAGAGCGAGTCCAATTCCACGCCCTCGTGCTGTCATATCTAGTCCTGAAAATGACTTAATGATTGGGAGCAGAAACAAGACTGGAAATGGAAGAGTTTACGTATCGAAGAATAGTACTGCTTTACCAAATAGACATGCTCTTGCTCAATGTAAAGTGAAATCACATGACACTACCGATATTCCTCCGGACGCAAGAAAATCCACAGAGCCCAAGTCTAAAGATAAAGTTGATCATGTAGGAAAGAAAAAGGTTCACAAAGGTAGCGTAAAATCTGATAATTTACATAGGCACTGGAAATTTTAG
Protein sequence

>Msa0076900

LTSDSSGSTEPEKDGDTDESKVYTRADLIPRVPVIEDFGEGGLTSNSSGSAEPEKDGDTDESKVYIRVDLIPRVPVTEDFGDGGLASDLSGPGGLEKDHGPEKDGHIDDEKKVNIRASPIPRPRAVISSPENDLMIGSRNKTGNGRVYVSKNSTALPNRHALAQCKVKSHDTTDIPPDARKSTEPKSKDKVDHVGKKKVHKGSVKSDNLHRHWKF*