AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0143200


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0143200 MtrunA17_Chr7g0266241 95.322 171 6 1 1 169 10 180 2.37e-117 330
Msa0143200 MtrunA17_Chr1g0178641 67.647 68 21 1 95 161 66 133 1.54e-27 100
Msa0143200 MtrunA17_Chr2g0321471 44.286 70 37 1 96 163 70 139 5.63e-12 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0143200 AT3G52740.1 49.180 122 52 2 57 171 18 136 6.44e-32 112
Msa0143200 AT3G44450.1 58.182 55 23 0 100 154 39 93 7.12e-16 70.1

Find 38 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCTTTATCATCATGGTTT+TGG 0.183603 1_4:-2129635 Msa0143190:three_prime_UTR
TCCTCTTGACCCCATATTTC+AGG 0.201417 1_4:-2129852 Msa0143190:three_prime_UTR
GAGTTTGGATTCCTGAAATA+TGG 0.212739 1_4:+2129841 Msa0143200:CDS
GATAGCTCCTGTTTCTTCTA+TGG 0.252691 1_4:+2129719 Msa0143200:CDS
TTGAAGTAGCTGGAAGAGTT+TGG 0.294041 1_4:+2129826 Msa0143200:CDS
CACTATGGCACGTGCTGCTT+TGG 0.339934 1_4:+2129935 Msa0143200:CDS
AGCAAAAGAGTTAACAACTA+TGG 0.376017 1_4:+2129752 Msa0143200:CDS
CACCTGTTGTGTATAGTTAG+AGG 0.380745 1_4:-2129987 Msa0143190:intron
CATCTTTATCTTTATCATCA+TGG 0.388480 1_4:-2129641 Msa0143190:three_prime_UTR
AAGAGGATTTATTGAAGGAT+TGG 0.393645 1_4:+2129868 Msa0143200:CDS
AGTTTGGATTCCTGAAATAT+GGG 0.425242 1_4:+2129842 Msa0143200:CDS
AGTTCCAAGCAAGATCACTA+TGG 0.434204 1_4:+2129920 Msa0143200:CDS
ACATCATCCATAGAAGAAAC+AGG 0.460873 1_4:-2129726 Msa0143190:three_prime_UTR
CTCCTCTAACTATACACAAC+AGG 0.463941 1_4:+2129985 Msa0143200:CDS
GGGTCAAGAGGATTTATTGA+AGG 0.475977 1_4:+2129863 Msa0143200:CDS
CAAGATTCCTTCACACCCAT+TGG 0.482903 1_4:+2129530 Msa0143200:CDS
GTGATCTTGCTTGGAACTAA+AGG 0.483762 1_4:-2129915 Msa0143190:three_prime_UTR
TCCTTGTTGCAAAGAAGAGA+TGG 0.484001 1_4:-2129675 Msa0143190:three_prime_UTR
AGGCATAGAGTTGAAGTAGC+TGG 0.488896 1_4:+2129816 Msa0143200:CDS
AACAACTATGGATGAAGTAT+TGG 0.489801 1_4:+2129764 Msa0143200:CDS
CTCATCTAAAGATGTCAACT+TGG 0.490619 1_4:+2129569 Msa0143200:CDS
CCTTGTTGCAAAGAAGAGAT+GGG 0.508262 1_4:-2129674 Msa0143190:three_prime_UTR
CATCAGATTCAACAGAGGAT+TGG 0.517512 1_4:-2129506 Msa0143190:three_prime_UTR
CGTGCCATAGTGATCTTGCT+TGG 0.523167 1_4:-2129924 Msa0143190:three_prime_UTR
TATAGTTAGAGGAGCAGCAT+TGG 0.530992 1_4:-2129976 Msa0143190:three_prime_UTR
CCCATCTCTTCTTTGCAACA+AGG 0.533413 1_4:+2129674 Msa0143200:CDS
TTTGAGTCCAATGGGTGTGA+AGG 0.539347 1_4:-2129537 Msa0143190:three_prime_UTR
GTTGACATCTTTAGATGAGT+GGG 0.544029 1_4:-2129565 Msa0143190:three_prime_UTR
GTGAAAGAGAGAGGCTGAAG+AGG 0.548904 1_4:+2129796 Msa0143200:CDS
TCCTGAAATATGGGGTCAAG+AGG 0.551314 1_4:+2129851 Msa0143200:CDS
TATGGATGAAGTATTGGAAG+AGG 0.560016 1_4:+2129770 Msa0143200:CDS
AGTTGACATCTTTAGATGAG+TGG 0.576084 1_4:-2129566 Msa0143190:three_prime_UTR
CGTGCTGCTTTGGTTGAAGA+GGG 0.579273 1_4:+2129945 Msa0143200:CDS
AAGAGGATAGTGAAAGAGAG+AGG 0.580510 1_4:+2129787 Msa0143200:CDS
ACGTGCTGCTTTGGTTGAAG+AGG 0.593330 1_4:+2129944 Msa0143200:CDS
TTGACATCTTTAGATGAGTG+GGG 0.627794 1_4:-2129564 Msa0143190:three_prime_UTR
GTTTGGATTCCTGAAATATG+GGG 0.638334 1_4:+2129843 Msa0143200:CDS
CTTGTCATCAGATTCAACAG+AGG 0.696844 1_4:-2129511 Msa0143190:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATCTTTATCATCATGGTTT+TGG - chr1_4:2129638-2129657 Msa0143190:three_prime_UTR 25.0%
! CATCTTTATCTTTATCATCA+TGG - chr1_4:2129644-2129663 Msa0143190:three_prime_UTR 25.0%
TTTGAGTGTTCAAAAAAGGA+GGG - chr1_4:2129611-2129630 Msa0143190:three_prime_UTR 30.0%
!! TTTTGAGTGTTCAAAAAAGG+AGG - chr1_4:2129612-2129631 Msa0143190:three_prime_UTR 30.0%
!!! TCCTTTTTTGAACACTCAAA+AGG + chr1_4:2129611-2129630 Msa0143200:CDS 30.0%
!! ACCTTTTGAGTGTTCAAAAA+AGG - chr1_4:2129615-2129634 Msa0143190:three_prime_UTR 30.0%
AGCAAAAGAGTTAACAACTA+TGG + chr1_4:2129752-2129771 Msa0143200:CDS 30.0%
AACAACTATGGATGAAGTAT+TGG + chr1_4:2129764-2129783 Msa0143200:CDS 30.0%
AGTTTGGATTCCTGAAATAT+GGG + chr1_4:2129842-2129861 Msa0143200:CDS 30.0%
AAGAGGATTTATTGAAGGAT+TGG + chr1_4:2129868-2129887 Msa0143200:CDS 30.0%
TTGACATCTTTAGATGAGTG+GGG - chr1_4:2129567-2129586 Msa0143190:three_prime_UTR 35.0%
GTTGACATCTTTAGATGAGT+GGG - chr1_4:2129568-2129587 Msa0143190:three_prime_UTR 35.0%
AGTTGACATCTTTAGATGAG+TGG - chr1_4:2129569-2129588 Msa0143190:three_prime_UTR 35.0%
CTCATCTAAAGATGTCAACT+TGG + chr1_4:2129569-2129588 Msa0143200:CDS 35.0%
ACATCATCCATAGAAGAAAC+AGG - chr1_4:2129729-2129748 Msa0143190:three_prime_UTR 35.0%
!! TATGGATGAAGTATTGGAAG+AGG + chr1_4:2129770-2129789 Msa0143200:CDS 35.0%
GAGTTTGGATTCCTGAAATA+TGG + chr1_4:2129841-2129860 Msa0143200:CDS 35.0%
GTTTGGATTCCTGAAATATG+GGG + chr1_4:2129843-2129862 Msa0143200:CDS 35.0%
CATCAGATTCAACAGAGGAT+TGG - chr1_4:2129509-2129528 Msa0143190:three_prime_UTR 40.0%
CTTGTCATCAGATTCAACAG+AGG - chr1_4:2129514-2129533 Msa0143190:three_prime_UTR 40.0%
!!! GGGGTTTTTTTGAGTCCAAT+GGG - chr1_4:2129548-2129567 Msa0143190:three_prime_UTR 40.0%
!!! TGGGGTTTTTTTGAGTCCAA+TGG - chr1_4:2129549-2129568 Msa0143190:three_prime_UTR 40.0%
CCTTGTTGCAAAGAAGAGAT+GGG - chr1_4:2129677-2129696 Msa0143190:three_prime_UTR 40.0%
TCCTTGTTGCAAAGAAGAGA+TGG - chr1_4:2129678-2129697 Msa0143190:three_prime_UTR 40.0%
! GATAGCTCCTGTTTCTTCTA+TGG + chr1_4:2129719-2129738 Msa0143200:CDS 40.0%
AAGAGGATAGTGAAAGAGAG+AGG + chr1_4:2129787-2129806 Msa0143200:CDS 40.0%
TTGAAGTAGCTGGAAGAGTT+TGG + chr1_4:2129826-2129845 Msa0143200:CDS 40.0%
GGGTCAAGAGGATTTATTGA+AGG + chr1_4:2129863-2129882 Msa0143200:CDS 40.0%
GTGATCTTGCTTGGAACTAA+AGG - chr1_4:2129918-2129937 Msa0143190:three_prime_UTR 40.0%
AGTTCCAAGCAAGATCACTA+TGG + chr1_4:2129920-2129939 Msa0143200:CDS 40.0%
TATAGTTAGAGGAGCAGCAT+TGG - chr1_4:2129979-2129998 Msa0143190:three_prime_UTR 40.0%
CTCCTCTAACTATACACAAC+AGG + chr1_4:2129985-2130004 Msa0143200:CDS 40.0%
CACCTGTTGTGTATAGTTAG+AGG - chr1_4:2129990-2130009 Msa0143190:intron 40.0%
CAAGATTCCTTCACACCCAT+TGG + chr1_4:2129530-2129549 Msa0143200:CDS 45.0%
TTTGAGTCCAATGGGTGTGA+AGG - chr1_4:2129540-2129559 Msa0143190:three_prime_UTR 45.0%
CCCATCTCTTCTTTGCAACA+AGG + chr1_4:2129674-2129693 Msa0143200:CDS 45.0%
AGGCATAGAGTTGAAGTAGC+TGG + chr1_4:2129816-2129835 Msa0143200:CDS 45.0%
TCCTGAAATATGGGGTCAAG+AGG + chr1_4:2129851-2129870 Msa0143200:CDS 45.0%
TCCTCTTGACCCCATATTTC+AGG - chr1_4:2129855-2129874 Msa0143190:three_prime_UTR 45.0%
GTGAAAGAGAGAGGCTGAAG+AGG + chr1_4:2129796-2129815 Msa0143200:CDS 50.0%
CGTGCCATAGTGATCTTGCT+TGG - chr1_4:2129927-2129946 Msa0143190:three_prime_UTR 50.0%
!! ACGTGCTGCTTTGGTTGAAG+AGG + chr1_4:2129944-2129963 Msa0143200:CDS 50.0%
!! CGTGCTGCTTTGGTTGAAGA+GGG + chr1_4:2129945-2129964 Msa0143200:CDS 50.0%
!! CACTATGGCACGTGCTGCTT+TGG + chr1_4:2129935-2129954 Msa0143200:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 2129498 2130013 2129498 ID=Msa0143200;Name=Msa0143200
chr1_4 mRNA 2129498 2130013 2129498 ID=Msa0143200-mRNA-1;Parent=Msa0143200;Name=Msa0143200-mRNA-1;_AED=0.37;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|171
chr1_4 exon 2129498 2130013 2129498 ID=Msa0143200-mRNA-1:exon:848;Parent=Msa0143200-mRNA-1
chr1_4 CDS 2129498 2130013 2129498 ID=Msa0143200-mRNA-1:cds;Parent=Msa0143200-mRNA-1
Gene Sequence

>Msa0143200

ATGTCTTACCAATCCTCTGTTGAATCTGATGACAAGATTCCTTCACACCCATTGGACTCAAAAAAACCCCACTCATCTAAAGATGTCAACTTGGAGACAAAACAAGATGACCCTCCTTTTTTGAACACTCAAAAGGTCCAAAACCATGATGATAAAGATAAAGATGCAACACAAAACCCATCTCTTCTTTGCAACAAGGAGAAAATGACACTATTGCAAGAGATAGCTCCTGTTTCTTCTATGGATGATGTTGAAGCAAAAGAGTTAACAACTATGGATGAAGTATTGGAAGAGGATAGTGAAAGAGAGAGGCTGAAGAGGCATAGAGTTGAAGTAGCTGGAAGAGTTTGGATTCCTGAAATATGGGGTCAAGAGGATTTATTGAAGGATTGGATAGATTGCACTGCATTTGATGCTCCTTTAGTTCCAAGCAAGATCACTATGGCACGTGCTGCTTTGGTTGAAGAGGGTACTAAAACCAATGCTGCTCCTCTAACTATACACAACAGGTGTTAG

Protein sequence

>Msa0143200

MSYQSSVESDDKIPSHPLDSKKPHSSKDVNLETKQDDPPFLNTQKVQNHDDKDKDATQNPSLLCNKEKMTLLQEIAPVSSMDDVEAKELTTMDEVLEEDSERERLKRHRVEVAGRVWIPEIWGQEDLLKDWIDCTAFDAPLVPSKITMARAALVEEGTKTNAAPLTIHNRC*