Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0530710 | XP_013454667.1 | 97.674 | 86 | 1 | 1 | 1 | 85 | 1 | 86 | 4.05e-51 | 165 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0530710 | A0A072UHU7 | 97.674 | 86 | 1 | 1 | 1 | 85 | 1 | 86 | 1.93e-51 | 165 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0010770 | Msa0530710 | 0.803215 | 3.877986e-49 | -8.615850e-47 |
| Msa0016510 | Msa0530710 | 0.807708 | 4.429664e-50 | -8.615850e-47 |
| Msa0045550 | Msa0530710 | 0.805816 | 1.111860e-49 | -8.615850e-47 |
| Msa0058310 | Msa0530710 | 0.822325 | 2.516505e-53 | -8.615850e-47 |
| Msa0074750 | Msa0530710 | 0.803908 | 2.784764e-49 | -8.615850e-47 |
| Msa0104800 | Msa0530710 | 0.807651 | 4.555274e-50 | -8.615850e-47 |
| Msa0106620 | Msa0530710 | 0.810840 | 9.436314e-51 | -8.615850e-47 |
| Msa0106710 | Msa0530710 | 0.815669 | 8.212788e-52 | -8.615850e-47 |
| Msa0138210 | Msa0530710 | 0.806208 | 9.196544e-50 | -8.615850e-47 |
| Msa0138640 | Msa0530710 | 0.814590 | 1.425598e-51 | -8.615850e-47 |
| Msa0174260 | Msa0530710 | 0.819110 | 1.379692e-52 | -8.615850e-47 |
| Msa0183420 | Msa0530710 | 0.818230 | 2.184750e-52 | -8.615850e-47 |
| Msa0187490 | Msa0530710 | 0.800395 | 1.470440e-48 | -8.615850e-47 |
| Msa0192640 | Msa0530710 | 0.813399 | 2.610646e-51 | -8.615850e-47 |
| Msa0207030 | Msa0530710 | 0.813009 | 3.178966e-51 | -8.615850e-47 |
| Msa0220730 | Msa0530710 | 0.813857 | 2.069915e-51 | -8.615850e-47 |
| Msa0232340 | Msa0530710 | 0.805502 | 1.294109e-49 | -8.615850e-47 |
| Msa0366210 | Msa0530710 | 0.802627 | 5.128840e-49 | -8.615850e-47 |
| Msa0376020 | Msa0530710 | 0.803457 | 3.454614e-49 | -8.615850e-47 |
| Msa0377440 | Msa0530710 | 0.817729 | 2.835879e-52 | -8.615850e-47 |
| Msa0387050 | Msa0530710 | 0.817784 | 2.755528e-52 | -8.615850e-47 |
| Msa0387810 | Msa0530710 | 0.808812 | 2.576820e-50 | -8.615850e-47 |
| Msa0390530 | Msa0530710 | 0.817272 | 3.594478e-52 | -8.615850e-47 |
| Msa0390960 | Msa0530710 | 0.806481 | 8.056545e-50 | -8.615850e-47 |
| Msa0395980 | Msa0530710 | 0.815088 | 1.106003e-51 | -8.615850e-47 |
| Msa0401560 | Msa0530710 | 0.813790 | 2.141714e-51 | -8.615850e-47 |
| Msa0403490 | Msa0530710 | 0.821239 | 4.488536e-53 | -8.615850e-47 |
| Msa0403770 | Msa0530710 | 0.809019 | 2.326659e-50 | -8.615850e-47 |
| Msa0431210 | Msa0530710 | 0.803262 | 3.791142e-49 | -8.615850e-47 |
| Msa0440510 | Msa0530710 | 0.806457 | 8.151404e-50 | -8.615850e-47 |
| Msa0443430 | Msa0530710 | 0.802558 | 5.300615e-49 | -8.615850e-47 |
| Msa0244970 | Msa0530710 | 0.813640 | 2.310595e-51 | -8.615850e-47 |
| Msa0244980 | Msa0530710 | 0.809193 | 2.135795e-50 | -8.615850e-47 |
| Msa0249310 | Msa0530710 | 0.803361 | 3.616045e-49 | -8.615850e-47 |
| Msa0271790 | Msa0530710 | 0.819686 | 1.019266e-52 | -8.615850e-47 |
| Msa0283420 | Msa0530710 | 0.812078 | 5.079771e-51 | -8.615850e-47 |
| Msa0285330 | Msa0530710 | 0.804063 | 2.585685e-49 | -8.615850e-47 |
| Msa0287790 | Msa0530710 | 0.803839 | 2.878034e-49 | -8.615850e-47 |
| Msa0308560 | Msa0530710 | 0.802429 | 5.634686e-49 | -8.615850e-47 |
| Msa0310430 | Msa0530710 | 0.824495 | 7.824691e-54 | -8.615850e-47 |
| Msa0311720 | Msa0530710 | 0.802529 | 5.372144e-49 | -8.615850e-47 |
| Msa0312910 | Msa0530710 | 0.804914 | 1.718630e-49 | -8.615850e-47 |
| Msa0321690 | Msa0530710 | 0.837049 | 6.537448e-57 | -8.615850e-47 |
| Msa0482940 | Msa0530710 | 0.839261 | 1.760882e-57 | -8.615850e-47 |
| Msa0491000 | Msa0530710 | 0.813042 | 3.126353e-51 | -8.615850e-47 |
| Msa0516020 | Msa0530710 | 0.803109 | 4.077624e-49 | -8.615850e-47 |
| Msa0517650 | Msa0530710 | 0.800483 | 1.410881e-48 | -8.615850e-47 |
| Msa0518170 | Msa0530710 | 0.800702 | 1.273445e-48 | -8.615850e-47 |
| Msa0520270 | Msa0530710 | 0.800410 | 1.460030e-48 | -8.615850e-47 |
| Msa0530710 | Msa0531250 | 0.802158 | 6.406041e-49 | -8.615850e-47 |
| Msa0530710 | Msa0566350 | 0.821624 | 3.657931e-53 | -8.615850e-47 |
| Msa0530710 | Msa0566750 | 0.816941 | 4.264333e-52 | -8.615850e-47 |
| Msa0530710 | Msa0569780 | 0.802675 | 5.013025e-49 | -8.615850e-47 |
| Msa0530710 | Msa0587540 | 0.820352 | 7.177784e-53 | -8.615850e-47 |
| Msa0530710 | Msa0588090 | 0.800950 | 1.133354e-48 | -8.615850e-47 |
| Msa0530710 | Msa0600450 | 0.806613 | 7.555249e-50 | -8.615850e-47 |
| Msa0530710 | Msa0621600 | 0.805018 | 1.634419e-49 | -8.615850e-47 |
| Msa0530710 | Msa0621840 | 0.821008 | 5.071761e-53 | -8.615850e-47 |
| Msa0530710 | Msa0628420 | 0.802578 | 5.248874e-49 | -8.615850e-47 |
| Msa0530710 | Msa0628500 | 0.806994 | 6.277264e-50 | -8.615850e-47 |
| Msa0530710 | Msa0640940 | 0.809236 | 2.090549e-50 | -8.615850e-47 |
| Msa0530710 | Msa0658680 | 0.817123 | 3.881703e-52 | -8.615850e-47 |
| Msa0530710 | Msa0663230 | 0.805596 | 1.236867e-49 | -8.615850e-47 |
| Msa0530710 | Msa0665520 | 0.834783 | 2.454496e-56 | -8.615850e-47 |
| Msa0530710 | Msa0679360 | 0.811188 | 7.933434e-51 | -8.615850e-47 |
| Msa0530710 | Msa0705990 | 0.820412 | 6.952767e-53 | -8.615850e-47 |
| Msa0530710 | Msa0709160 | 0.804675 | 1.927799e-49 | -8.615850e-47 |
| Msa0530710 | Msa0721280 | 0.813759 | 2.175160e-51 | -8.615850e-47 |
| Msa0530710 | Msa0724170 | 0.801650 | 8.146876e-49 | -8.615850e-47 |
| Msa0530710 | Msa0732600 | 0.803829 | 2.892624e-49 | -8.615850e-47 |
| Msa0530710 | Msa0756380 | 0.801091 | 1.060501e-48 | -8.615850e-47 |
| Msa0530710 | Msa0773590 | 0.806422 | 8.291276e-50 | -8.615850e-47 |
| Msa0530710 | Msa0789560 | 0.829542 | 4.856624e-55 | -8.615850e-47 |
| Msa0530710 | Msa0811040 | 0.812508 | 4.093612e-51 | -8.615850e-47 |
| Msa0530710 | Msa0842770 | 0.806518 | 7.912325e-50 | -8.615850e-47 |
| Msa0530710 | Msa0844610 | 0.801534 | 8.606059e-49 | -8.615850e-47 |
| Msa0530710 | Msa0866400 | 0.810653 | 1.035518e-50 | -8.615850e-47 |
| Msa0530710 | Msa0958030 | 0.813609 | 2.347163e-51 | -8.615850e-47 |
| Msa0530710 | Msa0981310 | 0.804412 | 2.187176e-49 | -8.615850e-47 |
| Msa0530710 | Msa0985180 | 0.810527 | 1.102559e-50 | -8.615850e-47 |
| Msa0530710 | Msa0985220 | 0.800728 | 1.257885e-48 | -8.615850e-47 |
| Msa0530710 | Msa0992580 | 0.821731 | 3.454590e-53 | -8.615850e-47 |
| Msa0530710 | Msa0992600 | 0.805864 | 1.086588e-49 | -8.615850e-47 |
| Msa0530710 | Msa0994370 | 0.825760 | 3.931119e-54 | -8.615850e-47 |
| Msa0530710 | Msa1024680 | 0.820841 | 5.542245e-53 | -8.615850e-47 |
| Msa0530710 | Msa1026640 | 0.815729 | 7.961344e-52 | -8.615850e-47 |
| Msa0530710 | Msa1036300 | 0.805061 | 1.601152e-49 | -8.615850e-47 |
| Msa0530710 | Msa1039760 | 0.800158 | 1.643092e-48 | -8.615850e-47 |
| Msa0530710 | Msa1042660 | 0.803947 | 2.733844e-49 | -8.615850e-47 |
| Msa0530710 | Msa1063420 | 0.854341 | 1.313378e-61 | -8.615850e-47 |
| Msa0530710 | Msa1077070 | 0.812486 | 4.138585e-51 | -8.615850e-47 |
| Msa0530710 | Msa1122780 | 0.807180 | 5.733397e-50 | -8.615850e-47 |
| Msa0530710 | Msa1127430 | 0.803650 | 3.149702e-49 | -8.615850e-47 |
| Msa0530710 | Msa1130530 | 0.823020 | 1.733884e-53 | -8.615850e-47 |
| Msa0530710 | Msa1143020 | 0.800822 | 1.203330e-48 | -8.615850e-47 |
| Msa0530710 | Msa1178580 | 0.801903 | 7.229550e-49 | -8.615850e-47 |
| Msa0530710 | Msa1207740 | 0.811269 | 7.617882e-51 | -8.615850e-47 |
| Msa0530710 | Msa1208790 | 0.819724 | 9.993496e-53 | -8.615850e-47 |
| Msa0530710 | Msa1209190 | 0.812221 | 4.728807e-51 | -8.615850e-47 |
| Msa0530710 | Msa1231810 | 0.808741 | 2.668477e-50 | -8.615850e-47 |
| Msa0530710 | Msa1233850 | 0.800344 | 1.506485e-48 | -8.615850e-47 |
| Msa0530710 | Msa1250110 | 0.801898 | 7.246446e-49 | -8.615850e-47 |
| Msa0530710 | Msa1250730 | 0.802781 | 4.767466e-49 | -8.615850e-47 |
| Msa0530710 | Msa1257070 | 0.818904 | 1.536747e-52 | -8.615850e-47 |
| Msa0530710 | Msa1258980 | 0.815488 | 9.008950e-52 | -8.615850e-47 |
| Msa0530710 | Msa1263290 | -0.809671 | 1.686510e-50 | -8.615850e-47 |
| Msa0530710 | Msa1266020 | 0.802912 | 4.478853e-49 | -8.615850e-47 |
| Msa0530710 | Msa1270660 | 0.861272 | 1.152812e-63 | -8.615850e-47 |
| Msa0530710 | Msa1270730 | 0.850933 | 1.232068e-60 | -8.615850e-47 |
| Msa0530710 | Msa1312500 | 0.809555 | 1.786221e-50 | -8.615850e-47 |
| Msa0530710 | Msa1320820 | 0.805420 | 1.346338e-49 | -8.615850e-47 |
| Msa0530710 | Msa1330630 | 0.816612 | 5.054380e-52 | -8.615850e-47 |
| Msa0530710 | Msa1332050 | 0.801696 | 7.972875e-49 | -8.615850e-47 |
| Msa0530710 | Msa1335230 | 0.841889 | 3.613004e-58 | -8.615850e-47 |
| Msa0530710 | Msa1336040 | 0.822274 | 2.585309e-53 | -8.615850e-47 |
| Msa0530710 | Msa1337170 | 0.814329 | 1.628393e-51 | -8.615850e-47 |
| Msa0530710 | Msa1341490 | 0.822222 | 2.658623e-53 | -8.615850e-47 |
| Msa0530710 | Msa1357890 | 0.817487 | 3.215655e-52 | -8.615850e-47 |
| Msa0530710 | Msa1359920 | 0.802553 | 5.312760e-49 | -8.615850e-47 |
| Msa0530710 | Msa1370590 | 0.839500 | 1.527102e-57 | -8.615850e-47 |
| Msa0530710 | Msa1372290 | 0.828204 | 1.023923e-54 | -8.615850e-47 |
| Msa0530710 | Msa1373850 | 0.801998 | 6.911685e-49 | -8.615850e-47 |
| Msa0530710 | Msa1384100 | 0.839605 | 1.433773e-57 | -8.615850e-47 |
| Msa0530710 | Msa1391720 | 0.809726 | 1.640584e-50 | -8.615850e-47 |
| Msa0530710 | Msa1391750 | 0.802094 | 6.603679e-49 | -8.615850e-47 |
| Msa0530710 | Msa1398090 | 0.807859 | 4.113475e-50 | -8.615850e-47 |
| Msa0530710 | Msa1407600 | 0.830218 | 3.323862e-55 | -8.615850e-47 |
| Msa0530710 | Msa1410390 | 0.803314 | 3.697984e-49 | -8.615850e-47 |
| Msa0530710 | Msa1423820 | 0.821289 | 4.370248e-53 | -8.615850e-47 |
| Msa0530710 | Msa1426570 | 0.817830 | 2.690549e-52 | -8.615850e-47 |
| Msa0530710 | Msa1428780 | 0.805418 | 1.347964e-49 | -8.615850e-47 |
| Msa0530710 | Msa1442790 | 0.819447 | 1.156042e-52 | -8.615850e-47 |
| Msa0530710 | Msa1444120 | 0.840192 | 1.008339e-57 | -8.615850e-47 |
| Msa0530710 | Msa1448490 | 0.803149 | 4.000777e-49 | -8.615850e-47 |
| Msa0530710 | Msa1454960 | 0.803355 | 3.626192e-49 | -8.615850e-47 |
| Msa0530710 | Msa1456230 | 0.808720 | 2.695875e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0530710 | MtrunA17_Chr4g0003591 | 97.674 | 86 | 1 | 1 | 1 | 85 | 1 | 86 | 3.72e-55 | 165 |
| Msa0530710 | MtrunA17_Chr2g0332831 | 56.250 | 96 | 29 | 4 | 1 | 84 | 1 | 95 | 9.41e-26 | 91.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTGCTGATTGTGGCTTGA+TGG | 0.224833 | 4_1:+4774435 | None:intergenic |
| ATGCCTTCATTAAGAACTTT+AGG | 0.365751 | 4_1:-4774290 | Msa0530710:CDS |
| CTTCCTTTCCTTGCTGATTG+TGG | 0.393281 | 4_1:+4774427 | None:intergenic |
| ATTATGAGAAAATGCTTGCT+AGG | 0.453535 | 4_1:-4774230 | Msa0530710:CDS |
| CTGCCTAAAGTTCTTAATGA+AGG | 0.506137 | 4_1:+4774287 | None:intergenic |
| TTATGAGAAAATGCTTGCTA+GGG | 0.510063 | 4_1:-4774229 | Msa0530710:CDS |
| GCATTGATATCTTCTTCACT+TGG | 0.515090 | 4_1:+4774321 | None:intergenic |
| GGCAGCAGCTTATGCTTCAG+AGG | 0.527016 | 4_1:-4774269 | Msa0530710:CDS |
| TTGCAAATAGCTCTTTCTCA+TGG | 0.538159 | 4_1:+4774458 | None:intergenic |
| AGAAAATGCTTGCTAGGGGA+CGG | 0.603069 | 4_1:-4774224 | Msa0530710:CDS |
| ATCAAACAAGAAAGCTAGAG+AGG | 0.612578 | 4_1:-4774347 | Msa0530710:CDS |
| AAGCCACAATCAGCAAGGAA+AGG | 0.618413 | 4_1:-4774430 | Msa0530710:CDS |
| GTCGTCGTCGTCTTCGCATG+AGG | 0.619506 | 4_1:-4774388 | Msa0530710:CDS |
| CCATCAAGCCACAATCAGCA+AGG | 0.651776 | 4_1:-4774435 | Msa0530710:CDS |
| TATGAGAAAATGCTTGCTAG+GGG | 0.668349 | 4_1:-4774228 | Msa0530710:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATTTTCTCATAATTCTCAA+TGG | + | chr4_1:4774441-4774460 | None:intergenic | 25.0% |
| ATGCCTTCATTAAGAACTTT+AGG | - | chr4_1:4774387-4774406 | Msa0530710:CDS | 30.0% | |
| ATTATGAGAAAATGCTTGCT+AGG | - | chr4_1:4774447-4774466 | Msa0530710:CDS | 30.0% | |
| ! | TTATGAGAAAATGCTTGCTA+GGG | - | chr4_1:4774448-4774467 | Msa0530710:CDS | 30.0% |
| ATCAAACAAGAAAGCTAGAG+AGG | - | chr4_1:4774330-4774349 | Msa0530710:CDS | 35.0% | |
| ! | GCATTGATATCTTCTTCACT+TGG | + | chr4_1:4774359-4774378 | None:intergenic | 35.0% |
| CTGCCTAAAGTTCTTAATGA+AGG | + | chr4_1:4774393-4774412 | None:intergenic | 35.0% | |
| ! | TATGAGAAAATGCTTGCTAG+GGG | - | chr4_1:4774449-4774468 | Msa0530710:CDS | 35.0% |
| AAGCCACAATCAGCAAGGAA+AGG | - | chr4_1:4774247-4774266 | Msa0530710:CDS | 45.0% | |
| ! | CTTCCTTTCCTTGCTGATTG+TGG | + | chr4_1:4774253-4774272 | None:intergenic | 45.0% |
| ! | AGAAAATGCTTGCTAGGGGA+CGG | - | chr4_1:4774453-4774472 | Msa0530710:CDS | 45.0% |
| ! | CCTTGCTGATTGTGGCTTGA+TGG | + | chr4_1:4774245-4774264 | None:intergenic | 50.0% |
| CCATCAAGCCACAATCAGCA+AGG | - | chr4_1:4774242-4774261 | Msa0530710:CDS | 50.0% | |
| GGCAGCAGCTTATGCTTCAG+AGG | - | chr4_1:4774408-4774427 | Msa0530710:CDS | 55.0% | |
| GTCGTCGTCGTCTTCGCATG+AGG | - | chr4_1:4774289-4774308 | Msa0530710:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 4774221 | 4774478 | 4774221 | ID=Msa0530710;Name=Msa0530710 |
| chr4_1 | mRNA | 4774221 | 4774478 | 4774221 | ID=Msa0530710-mRNA-1;Parent=Msa0530710;Name=Msa0530710-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|85 |
| chr4_1 | exon | 4774221 | 4774478 | 4774221 | ID=Msa0530710-mRNA-1:exon:1443;Parent=Msa0530710-mRNA-1 |
| chr4_1 | CDS | 4774221 | 4774478 | 4774221 | ID=Msa0530710-mRNA-1:cds;Parent=Msa0530710-mRNA-1 |
| Gene Sequence |
| Protein sequence |
