AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1330630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1330630 MtrunA17_Chr4g0058261 85.841 113 8 1 1 105 15 127 3.92e-55 183
Msa1330630 MtrunA17_Chr4g0058261 95.652 92 4 0 104 195 238 329 1.49e-53 179
Msa1330630 MtrunA17_Chr4g0058281 71.560 109 23 2 1 103 1 107 4.94e-42 148
Msa1330630 MtrunA17_Chr4g0058281 66.667 93 29 2 104 195 220 311 4.08e-32 121
Msa1330630 MtrunA17_Chr3g0116721 49.580 119 51 3 79 196 52 162 2.26e-28 105
Msa1330630 MtrunA17_Chr4g0047661 43.411 129 66 3 18 144 26 149 6.59e-22 93.2
Msa1330630 MtrunA17_Chr4g0047631 32.338 201 99 6 18 183 27 225 1.76e-20 89.0
Msa1330630 MtrunA17_Chr5g0430081 51.111 90 38 2 18 103 9 96 2.04e-19 85.9
Msa1330630 MtrunA17_Chr5g0430171 54.118 85 35 3 23 105 18 100 3.74e-19 82.4
Msa1330630 MtrunA17_Chr4g0058231 53.763 93 35 4 15 105 5 91 4.20e-19 85.1
Msa1330630 MtrunA17_Chr5g0430051 58.209 67 26 1 37 103 35 99 4.91e-19 79.3
Msa1330630 MtrunA17_Chr3g0116741 47.312 93 44 1 104 196 227 314 5.68e-19 84.7
Msa1330630 MtrunA17_Chr3g0116741 51.724 87 38 2 19 105 32 114 1.71e-18 83.2
Msa1330630 MtrunA17_Chr4g0047691 42.963 135 62 5 19 144 25 153 8.25e-19 84.3
Msa1330630 MtrunA17_Chr4g0058201 45.536 112 40 4 32 127 23 129 1.06e-18 84.0
Msa1330630 MtrunA17_Chr4g0058201 36.364 88 53 1 109 196 207 291 9.81e-11 60.5
Msa1330630 MtrunA17_Chr4g0058191 52.747 91 36 3 15 105 5 88 1.45e-18 83.6
Msa1330630 MtrunA17_Chr4g0058191 37.500 88 52 1 109 196 205 289 7.37e-11 60.8
Msa1330630 MtrunA17_Chr5g0430111 54.023 87 37 2 19 105 17 100 1.93e-18 82.8
Msa1330630 MtrunA17_Chr3g0101631 42.000 150 65 7 14 143 7 154 2.16e-18 82.8
Msa1330630 MtrunA17_Chr4g0047641 48.889 90 41 3 19 105 26 113 3.85e-18 82.4
Msa1330630 MtrunA17_Chr4g0047641 31.818 88 60 0 109 196 228 315 2.94e-11 62.0
Msa1330630 MtrunA17_Chr5g0430131 54.023 87 37 2 19 105 17 100 5.16e-18 82.0
Msa1330630 MtrunA17_Chr3g0116681 43.119 109 48 4 1 103 1 101 6.49e-18 81.6
Msa1330630 MtrunA17_Chr3g0117321 60.000 65 24 1 41 105 8 70 1.80e-17 75.5
Msa1330630 MtrunA17_Chr5g0430031 58.108 74 28 2 33 105 29 100 1.94e-17 80.1
Msa1330630 MtrunA17_Chr8g0390881 38.889 126 73 3 19 144 16 137 1.97e-17 80.1
Msa1330630 MtrunA17_Chr5g0430101 51.724 87 39 2 19 105 16 99 2.18e-17 80.1
Msa1330630 MtrunA17_Chr5g0430091 52.941 85 36 3 23 105 18 100 4.48e-17 79.3
Msa1330630 MtrunA17_Chr3g0101601 51.136 88 36 3 23 105 15 100 6.42e-17 78.6
Msa1330630 MtrunA17_Chr3g0101611 53.425 73 32 1 31 103 29 99 6.55e-17 78.6
Msa1330630 MtrunA17_Chr7g0218601 42.045 88 49 1 111 198 218 303 1.23e-16 77.8
Msa1330630 MtrunA17_Chr3g0101691 49.474 95 43 3 14 105 10 102 1.31e-16 77.8
Msa1330630 MtrunA17_Chr3g0101571 58.209 67 26 1 37 103 33 97 2.72e-16 77.0
Msa1330630 MtrunA17_Chr3g0101581 52.632 76 33 2 29 103 25 98 2.76e-16 77.0
Msa1330630 MtrunA17_Chr4g0076161 63.077 65 19 2 42 103 39 101 3.33e-16 76.6
Msa1330630 MtrunA17_Chr6g0463791 53.846 91 36 3 17 103 11 99 4.25e-16 76.3
Msa1330630 MtrunA17_Chr3g0101621 47.826 92 41 3 19 105 8 97 5.72e-16 75.9
Msa1330630 MtrunA17_Chr3g0101831 38.028 142 76 6 19 157 14 146 1.69e-15 74.7
Msa1330630 MtrunA17_Chr5g0403331 44.444 108 44 4 42 141 37 136 2.07e-15 74.3
Msa1330630 MtrunA17_Chr3g0101731 48.276 87 41 3 19 103 14 98 2.08e-15 74.3
Msa1330630 MtrunA17_Chr6g0472311 37.121 132 72 4 15 141 14 139 3.25e-15 73.9
Msa1330630 MtrunA17_Chr3g0101711 44.554 101 44 3 15 105 3 101 3.25e-15 73.6
Msa1330630 MtrunA17_Chr3g0101651 60.000 65 23 2 42 105 40 102 3.58e-15 73.6
Msa1330630 MtrunA17_Chr3g0101761 48.837 86 41 2 19 103 14 97 6.30e-15 72.8
Msa1330630 MtrunA17_Chr6g0484811 39.130 115 67 2 30 144 27 138 6.88e-15 72.8
Msa1330630 MtrunA17_Chr3g0101751 47.253 91 41 3 18 103 16 104 7.54e-15 72.8
Msa1330630 MtrunA17_Chr6g0463611 49.462 93 41 3 15 103 23 113 7.78e-15 72.0
Msa1330630 MtrunA17_Chr8g0357421 48.936 94 35 5 21 103 8 99 8.48e-15 72.4
Msa1330630 MtrunA17_Chr3g0101701 47.423 97 40 3 18 105 8 102 1.29e-14 72.0
Msa1330630 MtrunA17_Chr3g0101661 47.423 97 46 3 12 105 8 102 1.37e-14 72.0
Msa1330630 MtrunA17_Chr8g0357431 48.936 94 35 5 21 103 8 99 1.38e-14 72.0
Msa1330630 MtrunA17_Chr8g0355961 46.341 82 42 1 25 106 26 105 1.39e-14 72.0
Msa1330630 MtrunA17_Chr5g0446151 36.667 120 72 3 26 144 27 143 1.87e-14 71.6
Msa1330630 MtrunA17_Chr1g0162801 47.312 93 39 4 21 105 11 101 2.83e-14 70.9
Msa1330630 MtrunA17_Chr3g0101721 48.864 88 40 4 19 103 14 99 5.15e-14 70.1
Msa1330630 MtrunA17_Chr5g0446161 35.965 114 69 3 32 144 33 143 6.07e-14 70.1
Msa1330630 MtrunA17_Chr7g0218611 39.175 97 56 3 102 198 209 302 6.18e-14 70.1
Msa1330630 MtrunA17_Chr3g0101821 46.512 86 43 2 19 103 14 97 7.16e-14 69.7
Msa1330630 MtrunA17_Chr8g0356001 45.556 90 45 3 19 106 18 105 8.67e-14 69.7
Msa1330630 MtrunA17_Chr6g0463801 37.143 105 60 4 42 144 39 139 2.18e-13 68.6
Msa1330630 MtrunA17_Chr2g0280261 38.462 104 60 3 42 144 36 136 2.51e-13 68.2
Msa1330630 MtrunA17_Chr5g0395331 45.238 84 44 1 23 106 20 101 2.55e-13 68.2
Msa1330630 MtrunA17_Chr5g0445751 38.835 103 59 3 42 143 46 145 2.66e-13 67.4
Msa1330630 MtrunA17_Chr3g0101811 29.825 171 109 5 31 199 27 188 3.52e-13 67.8
Msa1330630 MtrunA17_Chr4g0011861 36.842 95 57 1 109 203 212 303 5.83e-13 66.6
Msa1330630 MtrunA17_Chr1g0162811 44.086 93 42 4 19 103 9 99 6.80e-13 67.0
Msa1330630 MtrunA17_Chr8g0382901 48.913 92 41 3 15 105 469 555 1.48e-12 66.2
Msa1330630 MtrunA17_Chr5g0395321 41.667 84 47 1 23 106 25 106 1.60e-12 65.9
Msa1330630 MtrunA17_Chr5g0395381 41.667 84 47 1 23 106 25 106 2.16e-12 65.5
Msa1330630 MtrunA17_Chr4g0011871 38.636 88 51 1 109 196 212 296 2.27e-12 65.5
Msa1330630 MtrunA17_Chr1g0167321 43.878 98 45 3 16 105 3 98 2.50e-12 65.5
Msa1330630 MtrunA17_Chr5g0419691 36.364 88 53 1 109 196 211 295 3.04e-12 65.1
Msa1330630 MtrunA17_Chr5g0406651 39.326 89 49 2 109 196 212 296 3.16e-12 65.1
Msa1330630 MtrunA17_Chr1g0156101 36.364 88 56 0 109 196 223 310 4.62e-12 64.7
Msa1330630 MtrunA17_Chr1g0156101 50.685 73 30 3 32 103 27 94 6.24e-12 64.3
Msa1330630 MtrunA17_Chr5g0403351 34.132 167 88 7 13 166 2 159 4.63e-12 64.7
Msa1330630 MtrunA17_Chr5g0431641 42.708 96 46 4 15 103 8 101 5.58e-12 64.3
Msa1330630 MtrunA17_Chr1g0156081 35.227 88 57 0 109 196 247 334 6.05e-12 64.3
Msa1330630 MtrunA17_Chr1g0156081 50.667 75 33 3 31 103 46 118 2.16e-11 62.4
Msa1330630 MtrunA17_Chr6g0484841 49.254 67 32 1 39 105 36 100 6.16e-12 64.3
Msa1330630 MtrunA17_Chr8g0344181 35.000 100 62 2 42 141 40 136 6.67e-12 63.9
Msa1330630 MtrunA17_Chr1g0174481 42.169 83 46 1 21 103 15 95 7.28e-12 63.9
Msa1330630 MtrunA17_Chr5g0445441 47.126 87 40 3 21 103 58 142 7.47e-12 63.9
Msa1330630 MtrunA17_Chr1g0205361 42.353 85 47 1 20 104 14 96 7.74e-12 63.9
Msa1330630 MtrunA17_Chr5g0445451 45.977 87 41 3 21 103 17 101 8.13e-12 63.9
Msa1330630 MtrunA17_Chr4g0020041 45.455 88 43 3 19 103 14 99 8.56e-12 63.5
Msa1330630 MtrunA17_Chr8g0344191 37.143 105 60 3 14 117 45 144 9.39e-12 63.5
Msa1330630 MtrunA17_Chr5g0445381 44.944 89 43 3 19 103 46 132 9.40e-12 63.5
Msa1330630 MtrunA17_Chr8g0361471 53.968 63 26 2 42 103 36 96 2.20e-11 62.4
Msa1330630 MtrunA17_Chr6g0466331 53.968 63 26 2 42 103 36 96 2.30e-11 62.4
Msa1330630 MtrunA17_Chr1g0156061 55.556 63 25 2 42 103 57 117 2.41e-11 62.4
Msa1330630 MtrunA17_Chr4g0039901 47.945 73 36 1 34 106 29 99 3.19e-11 62.0
Msa1330630 MtrunA17_Chr7g0265611 39.024 82 47 2 23 103 28 107 3.55e-11 62.0
Msa1330630 MtrunA17_Chr5g0445461 45.977 87 41 3 21 103 17 101 3.71e-11 62.0
Msa1330630 MtrunA17_Chr8g0344151 33.000 100 64 2 42 141 38 134 4.42e-11 61.6
Msa1330630 MtrunA17_Chr3g0101671 43.617 94 42 3 19 103 9 100 5.49e-11 61.2
Msa1330630 MtrunA17_Chr3g0117311 47.826 69 31 1 129 197 58 121 6.08e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1330630 AT3G48320.1 54.118 85 34 2 21 105 16 95 4.46e-20 88.2
Msa1330630 AT3G48320.1 31.667 120 66 3 77 196 184 287 4.76e-12 64.7
Msa1330630 AT3G48270.1 38.926 149 69 5 31 159 22 168 2.73e-19 85.9
Msa1330630 AT4G20240.2 34.392 189 102 5 31 199 24 210 5.43e-19 84.7
Msa1330630 AT3G48280.1 61.333 75 21 2 39 113 29 95 7.46e-19 84.7
Msa1330630 AT4G20240.1 34.392 189 102 5 31 199 24 210 7.82e-19 84.3
Msa1330630 AT4G13310.2 47.170 106 50 3 42 147 34 133 1.83e-18 83.2
Msa1330630 AT3G26180.2 34.104 173 85 6 42 203 32 186 2.34e-18 82.8
Msa1330630 AT3G48310.1 39.597 149 65 5 31 159 26 169 3.18e-18 82.8
Msa1330630 AT3G48310.1 35.000 100 55 2 97 196 198 287 9.12e-12 63.9
Msa1330630 AT4G13310.1 47.170 106 50 3 42 147 34 133 3.61e-18 82.8
Msa1330630 AT3G48290.2 62.500 64 22 1 42 105 35 96 6.96e-18 82.0
Msa1330630 AT3G48290.1 62.500 64 22 1 42 105 35 96 8.05e-18 81.6
Msa1330630 AT2G24180.1 58.824 68 25 2 37 103 32 97 9.87e-18 81.6
Msa1330630 AT3G26300.1 40.152 132 71 4 15 141 1 129 1.05e-17 81.3
Msa1330630 AT3G26310.1 46.512 86 44 1 18 103 8 91 2.71e-17 80.1
Msa1330630 AT5G57260.1 58.462 65 25 1 39 103 29 91 2.99e-17 80.1
Msa1330630 AT3G26320.1 52.564 78 35 1 26 103 17 92 4.91e-17 79.3
Msa1330630 AT5G24960.2 61.290 62 22 1 42 103 35 94 5.13e-17 79.0
Msa1330630 AT5G24960.1 61.290 62 22 1 42 103 35 94 9.09e-17 78.6
Msa1330630 AT2G30770.1 61.290 62 22 1 42 103 35 94 1.02e-16 78.6
Msa1330630 AT2G30770.1 35.000 120 65 3 77 196 187 293 4.34e-13 67.8
Msa1330630 AT4G13290.1 53.947 76 33 1 28 103 21 94 1.39e-16 78.2
Msa1330630 AT5G24950.1 61.290 62 22 1 42 103 34 93 1.55e-16 77.8
Msa1330630 AT3G26170.1 51.807 83 35 3 21 103 14 91 2.47e-16 77.4
Msa1330630 AT2G45560.3 41.111 90 49 2 19 106 15 102 3.73e-16 75.1
Msa1330630 AT3G26180.1 56.452 62 25 1 42 103 32 91 4.54e-16 76.6
Msa1330630 AT3G26330.1 42.202 109 57 3 39 147 30 132 4.94e-16 76.6
Msa1330630 AT3G48300.1 50.667 75 35 1 31 105 20 92 5.97e-16 76.3
Msa1330630 AT1G33720.1 38.596 114 60 5 19 124 13 124 5.99e-16 76.3
Msa1330630 AT3G48300.2 50.667 75 35 1 31 105 22 94 5.99e-16 76.3
Msa1330630 AT5G42590.1 53.623 69 30 1 35 103 27 93 6.68e-16 76.3
Msa1330630 AT1G11610.1 59.677 62 23 1 42 103 35 94 7.85e-16 75.9
Msa1330630 AT1G11610.2 59.677 62 23 1 42 103 35 94 8.14e-16 75.9
Msa1330630 AT3G26290.1 39.823 113 65 2 31 143 22 131 8.75e-16 75.9
Msa1330630 AT3G26290.2 39.823 113 65 2 31 143 35 144 9.12e-16 75.9
Msa1330630 AT5G25140.1 42.697 89 46 2 19 104 5 91 9.31e-16 75.9
Msa1330630 AT3G26160.1 44.706 85 45 1 19 103 9 91 1.03e-15 75.5
Msa1330630 AT2G45560.2 41.111 90 49 2 19 106 15 102 1.06e-15 74.7
Msa1330630 AT2G30750.1 59.677 62 23 1 42 103 35 94 1.38e-15 75.1
Msa1330630 AT2G30750.1 31.667 120 69 2 77 196 187 293 6.89e-13 67.4
Msa1330630 AT2G45560.1 41.111 90 49 2 19 106 15 102 2.19e-15 74.7
Msa1330630 AT3G26220.1 50.000 86 37 2 18 103 11 90 3.50e-14 71.2
Msa1330630 AT2G02580.1 53.521 71 28 1 15 80 1 71 3.81e-14 70.9
Msa1330630 AT5G25180.1 44.578 83 43 2 22 103 10 90 7.51e-14 70.1
Msa1330630 AT3G26150.1 35.246 122 73 3 19 140 9 124 1.01e-13 69.7
Msa1330630 AT2G45550.1 35.870 92 53 3 19 106 13 102 1.59e-13 69.3
Msa1330630 AT3G26280.2 46.914 81 41 1 23 103 32 110 1.67e-13 69.3
Msa1330630 AT3G26280.1 46.914 81 41 1 23 103 15 93 2.03e-13 68.9
Msa1330630 AT4G20235.1 41.935 93 36 2 21 103 12 96 4.38e-13 67.4
Msa1330630 AT5G25120.1 43.210 81 44 1 23 103 12 90 5.09e-13 67.8
Msa1330630 AT5G25130.1 47.826 69 34 1 35 103 24 90 2.54e-12 65.9
Msa1330630 AT3G26270.1 51.613 62 28 1 42 103 34 93 1.19e-11 63.5
Msa1330630 AT4G15330.1 40.164 122 59 5 19 134 20 133 1.62e-11 63.2
Msa1330630 AT1G13090.1 35.938 128 77 3 42 167 30 154 1.72e-11 62.8
Msa1330630 AT3G53280.1 50.000 62 29 1 42 103 30 89 1.97e-11 63.2
Msa1330630 AT5G06900.1 34.513 113 71 2 20 132 14 123 2.02e-11 63.2
Msa1330630 AT1G13110.1 41.379 87 46 2 20 103 9 93 3.55e-11 62.4
Msa1330630 AT3G44250.1 51.613 62 28 1 42 103 30 89 3.85e-11 62.4
Msa1330630 AT1G13090.2 35.938 128 77 3 42 167 30 154 3.93e-11 62.0
Msa1330630 AT3G26190.1 50.000 62 29 1 42 103 30 89 5.25e-11 62.0

Find 40 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAGATTACTTTCCTTCTT+TGG 0.151596 8_4:-49846133 Msa1330630:CDS
CGAGACCTTTCCCTCAAATA+TGG 0.195183 8_4:-49846618 Msa1330630:CDS
TTTGGCTTTGAAGAAGAATA+TGG 0.257143 8_4:-49846019 Msa1330630:CDS
GGAGATTACTTTCCTTCTTT+GGG 0.279745 8_4:-49846132 Msa1330630:CDS
GGTGGAGATGGAAGTTGTTT+TGG 0.281394 8_4:+49846699 None:intergenic
AAGAAAAGAGATAGACAAAA+TGG 0.285118 8_4:+49846783 None:intergenic
CTCCAAGAGGGTGGCATGCT+TGG 0.343954 8_4:-49845949 Msa1330630:CDS
TTACCGATGATTGGTAGTTT+AGG 0.361952 8_4:+49846678 None:intergenic
AAGTGCTTTGATATCATTGT+TGG 0.399083 8_4:+49845915 None:intergenic
GAGACCTTTCCCTCAAATAT+GGG 0.404666 8_4:-49846617 Msa1330630:CDS
ATTACTTTCCTTCTTTGGGT+TGG 0.408209 8_4:-49846128 Msa1330630:CDS
TCAAGTGATTGAAGAGCGTT+TGG 0.412860 8_4:-49846037 Msa1330630:CDS
TGATATCAAAGCACTTCTAA+TGG 0.429025 8_4:-49845908 Msa1330630:CDS
GGTTGGATTGATGTTCTTAG+TGG 0.437187 8_4:-49846111 Msa1330630:CDS
AGCCAAGCATGCCACCCTCT+TGG 0.442016 8_4:+49845947 None:intergenic
ATTCAAGAAGAAAGGATTTG+TGG 0.448255 8_4:-49845986 Msa1330630:CDS
TGCCACCCTCTTGGAGTTGA+AGG 0.448266 8_4:+49845956 None:intergenic
ATTGGTAGTTTAGGTGGAGA+TGG 0.463457 8_4:+49846687 None:intergenic
AATGATCAATTCAAGAAGAA+AGG 0.479071 8_4:-49845994 Msa1330630:CDS
TGATGAAGATTACCGATGAT+TGG 0.486474 8_4:+49846669 None:intergenic
CATCATGTCCCCATATTTGA+GGG 0.497693 8_4:+49846608 None:intergenic
CCGATGATTGGTAGTTTAGG+TGG 0.498767 8_4:+49846681 None:intergenic
TTTGTGTTTAAGTTTGCCAT+TGG 0.505913 8_4:-49846744 Msa1330630:intron
ACATCATGTCCCCATATTTG+AGG 0.513127 8_4:+49846607 None:intergenic
GCTGATGAAACCACTAGAGT+TGG 0.532216 8_4:+49846558 None:intergenic
TGTCCCCATATTTGAGGGAA+AGG 0.539390 8_4:+49846613 None:intergenic
ACATCAATCCAACCCAAAGA+AGG 0.540139 8_4:+49846120 None:intergenic
GGACATGATGTTGTTACAGT+TGG 0.568049 8_4:-49846595 Msa1330630:CDS
GATGAAACCACTAGAGTTGG+AGG 0.569974 8_4:+49846561 None:intergenic
GACATGATGTTGTTACAGTT+GGG 0.571212 8_4:-49846594 Msa1330630:CDS
CCACCTAAACTACCAATCAT+CGG 0.595738 8_4:-49846681 Msa1330630:CDS
TTCATCAGCAGATGTAGCCA+TGG 0.599833 8_4:-49846544 Msa1330630:CDS
ATCGGTAATCTTCATCAGCT+AGG 0.613489 8_4:-49846663 Msa1330630:CDS
AAAACATCCTCCAACTCTAG+TGG 0.621813 8_4:-49846568 Msa1330630:CDS
AGACCTTTCCCTCAAATATG+GGG 0.623971 8_4:-49846616 Msa1330630:CDS
CTCCTTCAACTCCAAGAGGG+TGG 0.645137 8_4:-49845958 Msa1330630:CDS
GGGAAAGGTCTCGAAGAGAA+CGG 0.655738 8_4:+49846628 None:intergenic
TATTCTCCTTCAACTCCAAG+AGG 0.662228 8_4:-49845962 Msa1330630:CDS
ATTCTCCTTCAACTCCAAGA+GGG 0.676452 8_4:-49845961 Msa1330630:CDS
AGGTCTCGAAGAGAACGGTG+TGG 0.739210 8_4:+49846633 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGGAAAAATTGGAAAATTTA+AGG - chr8_4:49846589-49846608 Msa1330630:CDS 20.0%
!! TGGAAAAATTGGAAAATTTA+AGG - chr8_4:49846589-49846608 Msa1330630:CDS 20.0%
! AAGAAAAGAGATAGACAAAA+TGG + chr8_4:49845900-49845919 None:intergenic 25.0%
!! CATCCATACAATAAGATTTT+TGG + chr8_4:49846215-49846234 None:intergenic 25.0%
! ATTGTTTGTAAATGTACTCT+TGG - chr8_4:49846443-49846462 Msa1330630:intron 25.0%
! AATGATCAATTCAAGAAGAA+AGG - chr8_4:49846686-49846705 Msa1330630:CDS 25.0%
! AAGAAAAGAGATAGACAAAA+TGG + chr8_4:49845900-49845919 None:intergenic 25.0%
!! CATCCATACAATAAGATTTT+TGG + chr8_4:49846215-49846234 None:intergenic 25.0%
! ATTGTTTGTAAATGTACTCT+TGG - chr8_4:49846443-49846462 Msa1330630:intron 25.0%
! AATGATCAATTCAAGAAGAA+AGG - chr8_4:49846686-49846705 Msa1330630:CDS 25.0%
! TTTGTGTTTAAGTTTGCCAT+TGG - chr8_4:49845936-49845955 Msa1330630:CDS 30.0%
! TTTGGATTTGGATTTTCCAA+TGG + chr8_4:49845955-49845974 None:intergenic 30.0%
!!! TTTTGGATTGGTTTTGGATT+TGG + chr8_4:49845967-49845986 None:intergenic 30.0%
!!! AAGTTGTTTTGGATTGGTTT+TGG + chr8_4:49845973-49845992 None:intergenic 30.0%
! GTGATTTTTCATGATTTCCA+TGG + chr8_4:49846156-49846175 None:intergenic 30.0%
!!! ATTTTTGGAGCTATATGTTG+AGG + chr8_4:49846200-49846219 None:intergenic 30.0%
GCTCCAAAAATCTTATTGTA+TGG - chr8_4:49846209-49846228 Msa1330630:intron 30.0%
TTGGACTTTATGGAGAAAAA+TGG - chr8_4:49846249-49846268 Msa1330630:intron 30.0%
AGAAGAAGAAGTTGAAGAAT+TGG - chr8_4:49846343-49846362 Msa1330630:intron 30.0%
AAGTATGAAGGAGATAATGA+AGG - chr8_4:49846470-49846489 Msa1330630:intron 30.0%
GATGTTCTTAGTGGAAAAAT+TGG - chr8_4:49846578-49846597 Msa1330630:CDS 30.0%
!! TTTGGCTTTGAAGAAGAATA+TGG - chr8_4:49846661-49846680 Msa1330630:CDS 30.0%
ATTCAAGAAGAAAGGATTTG+TGG - chr8_4:49846694-49846713 Msa1330630:CDS 30.0%
AAGTGCTTTGATATCATTGT+TGG + chr8_4:49846768-49846787 None:intergenic 30.0%
! TGATATCAAAGCACTTCTAA+TGG - chr8_4:49846772-49846791 Msa1330630:intron 30.0%
! TTTGTGTTTAAGTTTGCCAT+TGG - chr8_4:49845936-49845955 Msa1330630:CDS 30.0%
! TTTGGATTTGGATTTTCCAA+TGG + chr8_4:49845955-49845974 None:intergenic 30.0%
!!! TTTTGGATTGGTTTTGGATT+TGG + chr8_4:49845967-49845986 None:intergenic 30.0%
!!! AAGTTGTTTTGGATTGGTTT+TGG + chr8_4:49845973-49845992 None:intergenic 30.0%
! GTGATTTTTCATGATTTCCA+TGG + chr8_4:49846156-49846175 None:intergenic 30.0%
!!! ATTTTTGGAGCTATATGTTG+AGG + chr8_4:49846200-49846219 None:intergenic 30.0%
GCTCCAAAAATCTTATTGTA+TGG - chr8_4:49846209-49846228 Msa1330630:intron 30.0%
TTGGACTTTATGGAGAAAAA+TGG - chr8_4:49846249-49846268 Msa1330630:intron 30.0%
AGAAGAAGAAGTTGAAGAAT+TGG - chr8_4:49846343-49846362 Msa1330630:intron 30.0%
AAGTATGAAGGAGATAATGA+AGG - chr8_4:49846470-49846489 Msa1330630:intron 30.0%
GATGTTCTTAGTGGAAAAAT+TGG - chr8_4:49846578-49846597 Msa1330630:CDS 30.0%
!! TTTGGCTTTGAAGAAGAATA+TGG - chr8_4:49846661-49846680 Msa1330630:CDS 30.0%
ATTCAAGAAGAAAGGATTTG+TGG - chr8_4:49846694-49846713 Msa1330630:CDS 30.0%
AAGTGCTTTGATATCATTGT+TGG + chr8_4:49846768-49846787 None:intergenic 30.0%
! TGATATCAAAGCACTTCTAA+TGG - chr8_4:49846772-49846791 Msa1330630:intron 30.0%
ACACAAACAACACACTTACA+AGG + chr8_4:49845923-49845942 None:intergenic 35.0%
!!! AGATGGAAGTTGTTTTGGAT+TGG + chr8_4:49845979-49845998 None:intergenic 35.0%
! TTACCGATGATTGGTAGTTT+AGG + chr8_4:49846005-49846024 None:intergenic 35.0%
TGATGAAGATTACCGATGAT+TGG + chr8_4:49846014-49846033 None:intergenic 35.0%
GACATGATGTTGTTACAGTT+GGG - chr8_4:49846086-49846105 Msa1330630:CDS 35.0%
! ACTCTTGGAAGAAAGTATGA+AGG - chr8_4:49846458-49846477 Msa1330630:intron 35.0%
GCAACGTGAAAAAATTAGCA+AGG - chr8_4:49846492-49846511 Msa1330630:intron 35.0%
CGTGAAAAAATTAGCAAGGA+AGG - chr8_4:49846496-49846515 Msa1330630:intron 35.0%
! TATCTTCAAGCTTTTGCAGT+TGG - chr8_4:49846527-49846546 Msa1330630:CDS 35.0%
TGGAGATTACTTTCCTTCTT+TGG - chr8_4:49846547-49846566 Msa1330630:CDS 35.0%
GGAGATTACTTTCCTTCTTT+GGG - chr8_4:49846548-49846567 Msa1330630:CDS 35.0%
ATTACTTTCCTTCTTTGGGT+TGG - chr8_4:49846552-49846571 Msa1330630:CDS 35.0%
ACACAAACAACACACTTACA+AGG + chr8_4:49845923-49845942 None:intergenic 35.0%
!!! AGATGGAAGTTGTTTTGGAT+TGG + chr8_4:49845979-49845998 None:intergenic 35.0%
! TTACCGATGATTGGTAGTTT+AGG + chr8_4:49846005-49846024 None:intergenic 35.0%
TGATGAAGATTACCGATGAT+TGG + chr8_4:49846014-49846033 None:intergenic 35.0%
GACATGATGTTGTTACAGTT+GGG - chr8_4:49846086-49846105 Msa1330630:CDS 35.0%
! ACTCTTGGAAGAAAGTATGA+AGG - chr8_4:49846458-49846477 Msa1330630:intron 35.0%
GCAACGTGAAAAAATTAGCA+AGG - chr8_4:49846492-49846511 Msa1330630:intron 35.0%
CGTGAAAAAATTAGCAAGGA+AGG - chr8_4:49846496-49846515 Msa1330630:intron 35.0%
! TATCTTCAAGCTTTTGCAGT+TGG - chr8_4:49846527-49846546 Msa1330630:CDS 35.0%
TGGAGATTACTTTCCTTCTT+TGG - chr8_4:49846547-49846566 Msa1330630:CDS 35.0%
GGAGATTACTTTCCTTCTTT+GGG - chr8_4:49846548-49846567 Msa1330630:CDS 35.0%
ATTACTTTCCTTCTTTGGGT+TGG - chr8_4:49846552-49846571 Msa1330630:CDS 35.0%
! ATTGGTAGTTTAGGTGGAGA+TGG + chr8_4:49845996-49846015 None:intergenic 40.0%
CCACCTAAACTACCAATCAT+CGG - chr8_4:49845999-49846018 Msa1330630:CDS 40.0%
ATCGGTAATCTTCATCAGCT+AGG - chr8_4:49846017-49846036 Msa1330630:CDS 40.0%
GAGACCTTTCCCTCAAATAT+GGG - chr8_4:49846063-49846082 Msa1330630:CDS 40.0%
AGACCTTTCCCTCAAATATG+GGG - chr8_4:49846064-49846083 Msa1330630:CDS 40.0%
CATCATGTCCCCATATTTGA+GGG + chr8_4:49846075-49846094 None:intergenic 40.0%
ACATCATGTCCCCATATTTG+AGG + chr8_4:49846076-49846095 None:intergenic 40.0%
GGACATGATGTTGTTACAGT+TGG - chr8_4:49846085-49846104 Msa1330630:CDS 40.0%
AAAACATCCTCCAACTCTAG+TGG - chr8_4:49846112-49846131 Msa1330630:CDS 40.0%
TTGGAGCTATATGTTGAGGT+CGG + chr8_4:49846196-49846215 None:intergenic 40.0%
! TTGTATGGATGTGATGACGT+TGG - chr8_4:49846224-49846243 Msa1330630:intron 40.0%
!!! GACGTTGGTTTTGGACTTTA+TGG - chr8_4:49846239-49846258 Msa1330630:intron 40.0%
ACATCAATCCAACCCAAAGA+AGG + chr8_4:49846563-49846582 None:intergenic 40.0%
GGTTGGATTGATGTTCTTAG+TGG - chr8_4:49846569-49846588 Msa1330630:CDS 40.0%
!! TCAAGTGATTGAAGAGCGTT+TGG - chr8_4:49846643-49846662 Msa1330630:CDS 40.0%
TATTCTCCTTCAACTCCAAG+AGG - chr8_4:49846718-49846737 Msa1330630:CDS 40.0%
ATTCTCCTTCAACTCCAAGA+GGG - chr8_4:49846719-49846738 Msa1330630:CDS 40.0%
! ATTGGTAGTTTAGGTGGAGA+TGG + chr8_4:49845996-49846015 None:intergenic 40.0%
CCACCTAAACTACCAATCAT+CGG - chr8_4:49845999-49846018 Msa1330630:CDS 40.0%
ATCGGTAATCTTCATCAGCT+AGG - chr8_4:49846017-49846036 Msa1330630:CDS 40.0%
GAGACCTTTCCCTCAAATAT+GGG - chr8_4:49846063-49846082 Msa1330630:CDS 40.0%
AGACCTTTCCCTCAAATATG+GGG - chr8_4:49846064-49846083 Msa1330630:CDS 40.0%
CATCATGTCCCCATATTTGA+GGG + chr8_4:49846075-49846094 None:intergenic 40.0%
ACATCATGTCCCCATATTTG+AGG + chr8_4:49846076-49846095 None:intergenic 40.0%
GGACATGATGTTGTTACAGT+TGG - chr8_4:49846085-49846104 Msa1330630:CDS 40.0%
AAAACATCCTCCAACTCTAG+TGG - chr8_4:49846112-49846131 Msa1330630:CDS 40.0%
TTGGAGCTATATGTTGAGGT+CGG + chr8_4:49846196-49846215 None:intergenic 40.0%
! TTGTATGGATGTGATGACGT+TGG - chr8_4:49846224-49846243 Msa1330630:intron 40.0%
!!! GACGTTGGTTTTGGACTTTA+TGG - chr8_4:49846239-49846258 Msa1330630:intron 40.0%
ACATCAATCCAACCCAAAGA+AGG + chr8_4:49846563-49846582 None:intergenic 40.0%
GGTTGGATTGATGTTCTTAG+TGG - chr8_4:49846569-49846588 Msa1330630:CDS 40.0%
!! TCAAGTGATTGAAGAGCGTT+TGG - chr8_4:49846643-49846662 Msa1330630:CDS 40.0%
TATTCTCCTTCAACTCCAAG+AGG - chr8_4:49846718-49846737 Msa1330630:CDS 40.0%
ATTCTCCTTCAACTCCAAGA+GGG - chr8_4:49846719-49846738 Msa1330630:CDS 40.0%
! GGTGGAGATGGAAGTTGTTT+TGG + chr8_4:49845984-49846003 None:intergenic 45.0%
! CCGATGATTGGTAGTTTAGG+TGG + chr8_4:49846002-49846021 None:intergenic 45.0%
CGAGACCTTTCCCTCAAATA+TGG - chr8_4:49846062-49846081 Msa1330630:CDS 45.0%
TGTCCCCATATTTGAGGGAA+AGG + chr8_4:49846070-49846089 None:intergenic 45.0%
GATGAAACCACTAGAGTTGG+AGG + chr8_4:49846122-49846141 None:intergenic 45.0%
GCTGATGAAACCACTAGAGT+TGG + chr8_4:49846125-49846144 None:intergenic 45.0%
TTCATCAGCAGATGTAGCCA+TGG - chr8_4:49846136-49846155 Msa1330630:CDS 45.0%
!!! GGATGTGATGACGTTGGTTT+TGG - chr8_4:49846230-49846249 Msa1330630:intron 45.0%
! GGTGGAGATGGAAGTTGTTT+TGG + chr8_4:49845984-49846003 None:intergenic 45.0%
! CCGATGATTGGTAGTTTAGG+TGG + chr8_4:49846002-49846021 None:intergenic 45.0%
CGAGACCTTTCCCTCAAATA+TGG - chr8_4:49846062-49846081 Msa1330630:CDS 45.0%
TGTCCCCATATTTGAGGGAA+AGG + chr8_4:49846070-49846089 None:intergenic 45.0%
GATGAAACCACTAGAGTTGG+AGG + chr8_4:49846122-49846141 None:intergenic 45.0%
GCTGATGAAACCACTAGAGT+TGG + chr8_4:49846125-49846144 None:intergenic 45.0%
TTCATCAGCAGATGTAGCCA+TGG - chr8_4:49846136-49846155 Msa1330630:CDS 45.0%
!!! GGATGTGATGACGTTGGTTT+TGG - chr8_4:49846230-49846249 Msa1330630:intron 45.0%
GGGAAAGGTCTCGAAGAGAA+CGG + chr8_4:49846055-49846074 None:intergenic 50.0%
GGGAAAGGTCTCGAAGAGAA+CGG + chr8_4:49846055-49846074 None:intergenic 50.0%
AGGTCTCGAAGAGAACGGTG+TGG + chr8_4:49846050-49846069 None:intergenic 55.0%
CTCCTTCAACTCCAAGAGGG+TGG - chr8_4:49846722-49846741 Msa1330630:CDS 55.0%
TGCCACCCTCTTGGAGTTGA+AGG + chr8_4:49846727-49846746 None:intergenic 55.0%
AGGTCTCGAAGAGAACGGTG+TGG + chr8_4:49846050-49846069 None:intergenic 55.0%
CTCCTTCAACTCCAAGAGGG+TGG - chr8_4:49846722-49846741 Msa1330630:CDS 55.0%
TGCCACCCTCTTGGAGTTGA+AGG + chr8_4:49846727-49846746 None:intergenic 55.0%
CTCCAAGAGGGTGGCATGCT+TGG - chr8_4:49846731-49846750 Msa1330630:CDS 60.0%
AGCCAAGCATGCCACCCTCT+TGG + chr8_4:49846736-49846755 None:intergenic 60.0%
CTCCAAGAGGGTGGCATGCT+TGG - chr8_4:49846731-49846750 Msa1330630:CDS 60.0%
AGCCAAGCATGCCACCCTCT+TGG + chr8_4:49846736-49846755 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 49845846 49846856 49845846 ID=Msa1330630;Name=Msa1330630
chr8_4 mRNA 49845846 49846856 49845846 ID=Msa1330630-mRNA-1;Parent=Msa1330630;Name=Msa1330630-mRNA-1;_AED=0.23;_eAED=0.23;_QI=0|0|0|1|0|0|3|0|216
chr8_4 exon 49846779 49846856 49846779 ID=Msa1330630-mRNA-1:exon:14479;Parent=Msa1330630-mRNA-1
chr8_4 exon 49846521 49846754 49846521 ID=Msa1330630-mRNA-1:exon:14478;Parent=Msa1330630-mRNA-1
chr8_4 exon 49845846 49846184 49845846 ID=Msa1330630-mRNA-1:exon:14477;Parent=Msa1330630-mRNA-1
chr8_4 CDS 49846779 49846856 49846779 ID=Msa1330630-mRNA-1:cds;Parent=Msa1330630-mRNA-1
chr8_4 CDS 49846521 49846754 49846521 ID=Msa1330630-mRNA-1:cds;Parent=Msa1330630-mRNA-1
chr8_4 CDS 49845846 49846184 49845846 ID=Msa1330630-mRNA-1:cds;Parent=Msa1330630-mRNA-1
Gene Sequence

>Msa1330630

ATGGCTCTTAAAAAATGGTCACATGAACAAATTACAGAAGCTTTGTCTTCTCCATTTTGTCTATCTCTTTTCTTCCTTTTTGCCATTGGAAAATCCAAATCCAAAACCAATCCAAAACAACTTCCATCTCCACCTAAACTACCAATCATCGGTAATCTTCATCAGCTAGGCACACTTCCACACCGTTCTCTTCGAGACCTTTCCCTCAAATATGGGGACATGATGTTGTTACAGTTGGGACAAAAACATCCTCCAACTCTAGTGGTTTCATCAGCAGATGTAGCCATGGAAATCATGAAAAATCACGATCGAGTTATGATTTATCTTCAAGCTTTTGCAGTTGGAGATTACTTTCCTTCTTTGGGTTGGATTGATGTTCTTAGTGGAAAAATTGGAAAATTTAAGGAAACTTTTCGAGAGCTTGATAGTTTGTTTGATCAAGTGATTGAAGAGCGTTTGGCTTTGAAGAAGAATATGGAAAATGATCAATTCAAGAAGAAAGGATTTGTGGATATTCTCCTTCAACTCCAAGAGGGTGGCATGCTTGGCTTTGAGCTCTCCAACAATGATATCAAAGCACTTCTAATGGTTCTCTCTCTTTTATCTCTTTTTTTGTCTCATTTACACTTTCACTTCCTCACTAGAAAATAA
Protein sequence

>Msa1330630

MALKKWSHEQITEALSSPFCLSLFFLFAIGKSKSKTNPKQLPSPPKLPIIGNLHQLGTLPHRSLRDLSLKYGDMMLLQLGQKHPPTLVVSSADVAMEIMKNHDRVMIYLQAFAVGDYFPSLGWIDVLSGKIGKFKETFRELDSLFDQVIEERLALKKNMENDQFKKKGFVDILLQLQEGGMLGFELSNNDIKALLMVLSLLSLFLSHLHFHFLTRK*