AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0390530


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0390530 MtrunA17_Chr7g0264131 95.783 166 1 2 1 166 1 160 1.20e-112 316
Msa0390530 MtrunA17_Chr2g0326661 43.312 157 73 5 11 166 15 156 1.07e-31 111
Msa0390530 MtrunA17_Chr4g0029031 47.101 138 55 5 36 165 31 158 5.19e-31 109
Msa0390530 MtrunA17_Chr4g0074781 35.979 189 83 8 1 165 1 175 1.42e-22 88.6
Msa0390530 MtrunA17_Chr2g0288341 48.235 85 39 2 82 165 137 217 2.54e-18 78.6
Msa0390530 MtrunA17_Chr2g0288501 52.326 86 33 2 83 164 73 154 6.00e-17 73.6
Msa0390530 MtrunA17_Chr3g0099101 40.000 110 37 4 84 164 87 196 1.47e-12 62.8
Msa0390530 MtrunA17_Chr2g0321611 39.286 84 46 2 84 163 170 252 2.25e-11 60.5
Msa0390530 MtrunA17_Chr7g0266691 60.417 48 15 1 123 166 170 217 2.59e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0390530 AT5G03230.1 40.571 175 83 5 3 166 2 166 4.20e-33 115
Msa0390530 AT3G45210.1 44.242 165 73 4 2 166 3 148 1.16e-30 108
Msa0390530 AT2G28400.1 39.412 170 91 3 1 166 1 162 1.88e-28 103
Msa0390530 AT5G60680.1 41.860 172 76 5 6 166 5 163 4.83e-28 102
Msa0390530 AT4G21970.1 50.538 93 27 4 83 164 59 143 3.17e-18 77.0
Msa0390530 AT4G04630.1 39.007 141 73 4 37 164 26 166 1.29e-17 75.9
Msa0390530 AT1G11700.1 44.792 96 43 2 76 164 107 199 2.39e-16 73.2
Msa0390530 AT1G61930.1 47.253 91 38 2 82 164 113 201 8.00e-16 72.0
Msa0390530 AT4G21930.1 44.681 94 39 5 81 164 91 181 3.01e-14 67.4
Msa0390530 AT4G26950.1 34.752 141 66 5 37 164 15 142 1.98e-13 64.3
Msa0390530 AT4G26950.2 34.752 141 66 5 37 164 15 142 1.98e-13 64.3
Msa0390530 AT3G15040.1 42.529 87 46 1 84 166 157 243 3.45e-13 65.5
Msa0390530 AT1G29640.1 40.367 109 44 4 65 164 41 137 2.93e-12 61.2

Find 29 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCAATCAATGGCTTCTTCT+AGG 0.289420 3_2:-1529908 None:intergenic
GTATTCATCCTTCAAAATCT+TGG 0.290307 3_2:+1529623 None:intergenic
GGGAAAGGAAGGACTTTAAA+AGG 0.299267 3_2:-1529480 Msa0390530:CDS
GTAGTGTAAGGAATGCTATT+TGG 0.358519 3_2:-1529446 Msa0390530:CDS
ATGCTATTTGGAAGAAAGTT+GGG 0.379786 3_2:-1529434 Msa0390530:CDS
GACCAATCTGGTATATTCAT+TGG 0.380128 3_2:+1529645 None:intergenic
AATGCTATTTGGAAGAAAGT+TGG 0.385752 3_2:-1529435 Msa0390530:CDS
TTCAAAATCTTGGACCAATC+TGG 0.413413 3_2:+1529633 None:intergenic
CTAGAAACTCTCTTCAAACC+TGG 0.465204 3_2:+1529717 None:intergenic
GTTTCTGTCTCTGAGGTAGT+TGG 0.466042 3_2:+1529852 None:intergenic
AGTTTGATGAAGCTGATGTA+TGG 0.474055 3_2:-1529791 Msa0390530:CDS
GAAGAGACTTGCGTAGTGTA+AGG 0.480322 3_2:-1529458 Msa0390530:CDS
AAATGTAACTTGTTCTTGAA+AGG 0.483499 3_2:+1529877 None:intergenic
CTTTCTGTTCATGAAGGGAA+AGG 0.500406 3_2:-1529495 Msa0390530:CDS
GAAGATGAAGGTGATTATGA+TGG 0.512537 3_2:-1529573 Msa0390530:CDS
GCTTCTCTTTCTGTTCATGA+AGG 0.513560 3_2:-1529501 Msa0390530:CDS
CTTCTCTTTCTGTTCATGAA+GGG 0.517982 3_2:-1529500 Msa0390530:CDS
TACCAATGAATATACCAGAT+TGG 0.518610 3_2:-1529647 Msa0390530:CDS
AGTAGTGATGATGAAGATGA+AGG 0.546106 3_2:-1529585 Msa0390530:CDS
GGTAATGAAGATGAAGCTAA+AGG 0.550449 3_2:+1529666 None:intergenic
CTGTTCATGAAGGGAAAGGA+AGG 0.560984 3_2:-1529491 Msa0390530:CDS
GAATATCTTGCTAGAACTAG+AGG 0.563251 3_2:-1529525 Msa0390530:CDS
TGAAGGTGATTATGATGGAG+GGG 0.591476 3_2:-1529568 Msa0390530:CDS
GAAATTTGTTTCTGTCTCTG+AGG 0.611033 3_2:+1529845 None:intergenic
GATGAAGGTGATTATGATGG+AGG 0.617604 3_2:-1529570 Msa0390530:CDS
ATGAAGGTGATTATGATGGA+GGG 0.625231 3_2:-1529569 Msa0390530:CDS
CTAGCAAGATATTCATGAGG+AGG 0.644815 3_2:+1529534 None:intergenic
AGGTGATTATGATGGAGGGG+TGG 0.659515 3_2:-1529565 Msa0390530:CDS
GTTCTAGCAAGATATTCATG+AGG 0.678087 3_2:+1529531 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATGTAACTTGTTCTTGAA+AGG + chr3_2:1529448-1529467 None:intergenic 25.0%
! TTCAATCAAAAATCATCACA+AGG - chr3_2:1529497-1529516 Msa0390530:CDS 25.0%
ACAAACATAGAGCCAAAAAA+GGG - chr3_2:1529578-1529597 Msa0390530:CDS 30.0%
! GAAGAGAGTTTCTAGAAAAA+TGG - chr3_2:1529613-1529632 Msa0390530:CDS 30.0%
TACCAATGAATATACCAGAT+TGG - chr3_2:1529675-1529694 Msa0390530:CDS 30.0%
GTATTCATCCTTCAAAATCT+TGG + chr3_2:1529702-1529721 None:intergenic 30.0%
! AATGCTATTTGGAAGAAAGT+TGG - chr3_2:1529887-1529906 Msa0390530:CDS 30.0%
! ATGCTATTTGGAAGAAAGTT+GGG - chr3_2:1529888-1529907 Msa0390530:CDS 30.0%
!! GAAATTTGTTTCTGTCTCTG+AGG + chr3_2:1529480-1529499 None:intergenic 35.0%
AGTTTGATGAAGCTGATGTA+TGG - chr3_2:1529531-1529550 Msa0390530:CDS 35.0%
!!! TTTTGGCTCTATGTTTGTGT+TGG + chr3_2:1529576-1529595 None:intergenic 35.0%
CACAAACATAGAGCCAAAAA+AGG - chr3_2:1529577-1529596 Msa0390530:CDS 35.0%
GGTAATGAAGATGAAGCTAA+AGG + chr3_2:1529659-1529678 None:intergenic 35.0%
GACCAATCTGGTATATTCAT+TGG + chr3_2:1529680-1529699 None:intergenic 35.0%
TTCAAAATCTTGGACCAATC+TGG + chr3_2:1529692-1529711 None:intergenic 35.0%
!! AGATTGGTCCAAGATTTTGA+AGG - chr3_2:1529691-1529710 Msa0390530:CDS 35.0%
! AGTAGTGATGATGAAGATGA+AGG - chr3_2:1529737-1529756 Msa0390530:CDS 35.0%
GAAGATGAAGGTGATTATGA+TGG - chr3_2:1529749-1529768 Msa0390530:CDS 35.0%
! ATGAAGGTGATTATGATGGA+GGG - chr3_2:1529753-1529772 Msa0390530:CDS 35.0%
GTTCTAGCAAGATATTCATG+AGG + chr3_2:1529794-1529813 None:intergenic 35.0%
GAATATCTTGCTAGAACTAG+AGG - chr3_2:1529797-1529816 Msa0390530:CDS 35.0%
CTTCTCTTTCTGTTCATGAA+GGG - chr3_2:1529822-1529841 Msa0390530:CDS 35.0%
GTAGTGTAAGGAATGCTATT+TGG - chr3_2:1529876-1529895 Msa0390530:CDS 35.0%
!!! AAACCTGGTACACCCTTTTT+TGG + chr3_2:1529593-1529612 None:intergenic 40.0%
CTAGAAACTCTCTTCAAACC+TGG + chr3_2:1529608-1529627 None:intergenic 40.0%
GATGAAGGTGATTATGATGG+AGG - chr3_2:1529752-1529771 Msa0390530:CDS 40.0%
! TGAAGGTGATTATGATGGAG+GGG - chr3_2:1529754-1529773 Msa0390530:CDS 40.0%
CTAGCAAGATATTCATGAGG+AGG + chr3_2:1529791-1529810 None:intergenic 40.0%
GCTTCTCTTTCTGTTCATGA+AGG - chr3_2:1529821-1529840 Msa0390530:CDS 40.0%
CTTTCTGTTCATGAAGGGAA+AGG - chr3_2:1529827-1529846 Msa0390530:CDS 40.0%
GGGAAAGGAAGGACTTTAAA+AGG - chr3_2:1529842-1529861 Msa0390530:CDS 40.0%
!! GTTTCTGTCTCTGAGGTAGT+TGG + chr3_2:1529473-1529492 None:intergenic 45.0%
CTGTTCATGAAGGGAAAGGA+AGG - chr3_2:1529831-1529850 Msa0390530:CDS 45.0%
GAAGAGACTTGCGTAGTGTA+AGG - chr3_2:1529864-1529883 Msa0390530:CDS 45.0%
! GAGCCAAAAAAGGGTGTACC+AGG - chr3_2:1529587-1529606 Msa0390530:CDS 50.0%
! AGGTGATTATGATGGAGGGG+TGG - chr3_2:1529757-1529776 Msa0390530:CDS 50.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 1529422 1529922 1529422 ID=Msa0390530;Name=Msa0390530
chr3_2 mRNA 1529422 1529922 1529422 ID=Msa0390530-mRNA-1;Parent=Msa0390530;Name=Msa0390530-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|166
chr3_2 exon 1529422 1529922 1529422 ID=Msa0390530-mRNA-1:exon:458;Parent=Msa0390530-mRNA-1
chr3_2 CDS 1529422 1529922 1529422 ID=Msa0390530-mRNA-1:cds;Parent=Msa0390530-mRNA-1
Gene Sequence

>Msa0390530

ATGGCTTCTTCTAGGAAGAGTTTCCTTTCAAGAACAAGTTACATTTTTCCAACTACCTCAGAGACAGAAACAAATTTCAATCAAAAATCATCACAAGGAAAAGAATTAGAGTTTGATGAAGCTGATGTATGGAACATGTCATATTCAAATTCCAACACAAACATAGAGCCAAAAAAGGGTGTACCAGGTTTGAAGAGAGTTTCTAGAAAAATGGAAGCTAATAATAAAGTTAATCCTTTAGCTTCATCTTCATTACCAATGAATATACCAGATTGGTCCAAGATTTTGAAGGATGAATACAAAAAGAAGAAAGAGAGTAGTGATGATGAAGATGAAGGTGATTATGATGGAGGGGTGGTTCAGTTACCTCCTCATGAATATCTTGCTAGAACTAGAGGAGCTTCTCTTTCTGTTCATGAAGGGAAAGGAAGGACTTTAAAAGGAAGAGACTTGCGTAGTGTAAGGAATGCTATTTGGAAGAAAGTTGGGTTTGAAGATTGA

Protein sequence

>Msa0390530

MASSRKSFLSRTSYIFPTTSETETNFNQKSSQGKELEFDEADVWNMSYSNSNTNIEPKKGVPGLKRVSRKMEANNKVNPLASSSLPMNIPDWSKILKDEYKKKKESSDDEDEGDYDGGVVQLPPHEYLARTRGASLSVHEGKGRTLKGRDLRSVRNAIWKKVGFED*