Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0658680 | XP_013446746.1 | 84.062 | 389 | 19 | 1 | 7 | 352 | 2 | 390 | 0.0 | 630 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0002560 | Msa0658680 | 0.830887 | 2.279304e-55 | -8.615850e-47 |
| Msa0003630 | Msa0658680 | 0.831311 | 1.793409e-55 | -8.615850e-47 |
| Msa0005270 | Msa0658680 | 0.803831 | 2.889774e-49 | -8.615850e-47 |
| Msa0008200 | Msa0658680 | 0.816091 | 6.611900e-52 | -8.615850e-47 |
| Msa0009100 | Msa0658680 | 0.809222 | 2.105216e-50 | -8.615850e-47 |
| Msa0010770 | Msa0658680 | 0.832501 | 9.119357e-56 | -8.615850e-47 |
| Msa0012710 | Msa0658680 | 0.830120 | 3.511452e-55 | -8.615850e-47 |
| Msa0015090 | Msa0658680 | 0.820228 | 7.663478e-53 | -8.615850e-47 |
| Msa0016510 | Msa0658680 | 0.814357 | 1.605320e-51 | -8.615850e-47 |
| Msa0017320 | Msa0658680 | 0.867530 | 1.279198e-65 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0658680 | MtrunA17_Chr8g0381611 | 84.062 | 389 | 19 | 1 | 7 | 352 | 2 | 390 | 0.0 | 630 |
| Msa0658680 | MtrunA17_Chr3g0123571 | 59.900 | 399 | 112 | 3 | 1 | 351 | 1 | 399 | 4.52e-168 | 474 |
| Msa0658680 | MtrunA17_Chr8g0381601 | 59.536 | 388 | 109 | 3 | 5 | 345 | 5 | 391 | 8.27e-164 | 463 |
| Msa0658680 | MtrunA17_Chr7g0227041 | 51.899 | 395 | 136 | 3 | 1 | 341 | 1 | 395 | 2.93e-140 | 404 |
| Msa0658680 | MtrunA17_Chr2g0326801 | 48.285 | 379 | 152 | 2 | 3 | 337 | 2 | 380 | 3.45e-127 | 370 |
| Msa0658680 | MtrunA17_Chr4g0028891 | 49.502 | 402 | 112 | 5 | 29 | 339 | 5 | 406 | 4.83e-125 | 365 |
| Msa0658680 | MtrunA17_Chr2g0281421 | 34.926 | 272 | 123 | 8 | 92 | 309 | 72 | 343 | 1.36e-42 | 152 |
| Msa0658680 | MtrunA17_Chr2g0276851 | 33.696 | 276 | 132 | 8 | 76 | 300 | 58 | 333 | 1.78e-42 | 152 |
| Msa0658680 | MtrunA17_Chr4g0047961 | 35.857 | 251 | 111 | 7 | 100 | 300 | 62 | 312 | 7.76e-42 | 149 |
| Msa0658680 | MtrunA17_Chr1g0189401 | 33.224 | 304 | 144 | 8 | 94 | 341 | 75 | 375 | 1.78e-41 | 149 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0658680 | AT3G27470.2 | 52.685 | 391 | 122 | 5 | 11 | 342 | 2 | 388 | 5.17e-143 | 410 |
| Msa0658680 | AT3G27470.1 | 52.685 | 391 | 122 | 5 | 11 | 342 | 2 | 388 | 5.17e-143 | 410 |
| Msa0658680 | AT3G27470.4 | 52.685 | 391 | 122 | 5 | 11 | 342 | 2 | 388 | 5.17e-143 | 410 |
| Msa0658680 | AT3G27470.3 | 52.685 | 391 | 122 | 5 | 11 | 342 | 2 | 388 | 5.17e-143 | 410 |
| Msa0658680 | AT1G13000.2 | 53.003 | 383 | 122 | 5 | 11 | 339 | 15 | 393 | 7.77e-143 | 410 |
| Msa0658680 | AT1G13000.5 | 53.003 | 383 | 122 | 5 | 11 | 339 | 15 | 393 | 7.77e-143 | 410 |
| Msa0658680 | AT1G13000.4 | 53.003 | 383 | 122 | 5 | 11 | 339 | 15 | 393 | 7.77e-143 | 410 |
| Msa0658680 | AT1G13000.1 | 53.003 | 383 | 122 | 5 | 11 | 339 | 15 | 393 | 7.77e-143 | 410 |
| Msa0658680 | AT3G27470.5 | 52.685 | 391 | 122 | 5 | 11 | 342 | 2 | 388 | 1.10e-142 | 410 |
| Msa0658680 | AT1G67850.4 | 53.403 | 382 | 124 | 3 | 17 | 346 | 1 | 380 | 5.28e-142 | 408 |
Find 112 sgRNAs with CRISPR-Local
Find 213 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATAAAATACGCAACCGCG+TGG | 0.661048 | 4_3:+68864295 | None:intergenic |
| AAGGATTCTGCTGCTTTGCT+CGG | 0.437840 | 4_3:+68860720 | None:intergenic |
| AAGTAACTTGCGGAATTGTT+AGG | 0.285302 | 4_3:-68864255 | Msa0658680:five_prime_UTR |
| AATGAATTTGTTCATGGATG+GGG | 0.522005 | 4_3:-68861479 | Msa0658680:CDS |
| AATTCCGCAAGTTACTTATA+TGG | 0.292250 | 4_3:+68864261 | None:intergenic |
| ACCAAGGTATTCCATCACTA+GGG | 0.546593 | 4_3:-68861291 | Msa0658680:CDS |
| ACCCAACAGTGAAGGTCACA+AGG | 0.602730 | 4_3:+68862676 | None:intergenic |
| ACCTTGTGACCTTCACTGTT+GGG | 0.430384 | 4_3:-68862677 | Msa0658680:CDS |
| ACCTTTGTTGTTGAGAATGT+TGG | 0.462834 | 4_3:+68863727 | None:intergenic |
| ACGATGGCCGAACAACCGAA+TGG | 0.557763 | 4_3:-68862501 | Msa0658680:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATTCCGCAAGTTACTTATA+TGG | + | chr4_3:68860685-68860704 | None:intergenic | 30.0% | |
| TACTTCCATTGTTTCAACAT+TGG | - | chr4_3:68860739-68860758 | Msa0658680:three_prime_UTR | 30.0% | |
| TATAAGTGTAACACAAGGAA+AGG | + | chr4_3:68860805-68860824 | None:intergenic | 30.0% | |
| TCTATCTTCTTTGCACTTTA+AGG | - | chr4_3:68860906-68860925 | Msa0658680:CDS | 30.0% | |
| GGATTTCTTGGCTTATTTAT+AGG | - | chr4_3:68861183-68861202 | Msa0658680:intron | 30.0% | |
| CAATCAATCACCAAAATAAG+TGG | + | chr4_3:68861815-68861834 | None:intergenic | 30.0% | |
| TTGTTTATACTGACCTCATT+GGG | + | chr4_3:68861937-68861956 | None:intergenic | 30.0% | |
| AAAAAAGTCTAGTAGCTAGA+TGG | + | chr4_3:68862189-68862208 | None:intergenic | 30.0% | |
| ATCCTTCTATTTCATTACGA+TGG | - | chr4_3:68862426-68862445 | Msa0658680:intron | 30.0% | |
| TTCCATGCTTACTCTTTAAA+AGG | - | chr4_3:68863284-68863303 | Msa0658680:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_3 | gene | 68860641 | 68864324 | 68860641 | ID=Msa0658680;Name=Msa0658680 |
| chr4_3 | mRNA | 68860641 | 68864324 | 68860641 | ID=Msa0658680-mRNA-1;Parent=Msa0658680;Name=Msa0658680-mRNA-1;_AED=0.10;_eAED=0.10;_QI=360|0.90|0.91|1|1|1|12|244|352 |
| chr4_3 | exon | 68860641 | 68861001 | 68860641 | ID=Msa0658680-mRNA-1:exon:20040;Parent=Msa0658680-mRNA-1 |
| chr4_3 | exon | 68861157 | 68861192 | 68861157 | ID=Msa0658680-mRNA-1:exon:20039;Parent=Msa0658680-mRNA-1 |
| chr4_3 | exon | 68861285 | 68861359 | 68861285 | ID=Msa0658680-mRNA-1:exon:20038;Parent=Msa0658680-mRNA-1 |
| chr4_3 | exon | 68861448 | 68861501 | 68861448 | ID=Msa0658680-mRNA-1:exon:20037;Parent=Msa0658680-mRNA-1 |
| chr4_3 | exon | 68861683 | 68861806 | 68861683 | ID=Msa0658680-mRNA-1:exon:20036;Parent=Msa0658680-mRNA-1 |
| chr4_3 | exon | 68861912 | 68862024 | 68861912 | ID=Msa0658680-mRNA-1:exon:20035;Parent=Msa0658680-mRNA-1 |
| chr4_3 | exon | 68862468 | 68862557 | 68862468 | ID=Msa0658680-mRNA-1:exon:20034;Parent=Msa0658680-mRNA-1 |
| chr4_3 | exon | 68862638 | 68862721 | 68862638 | ID=Msa0658680-mRNA-1:exon:20033;Parent=Msa0658680-mRNA-1 |
| chr4_3 | exon | 68863023 | 68863127 | 68863023 | ID=Msa0658680-mRNA-1:exon:20032;Parent=Msa0658680-mRNA-1 |
| chr4_3 | exon | 68863487 | 68863615 | 68863487 | ID=Msa0658680-mRNA-1:exon:20031;Parent=Msa0658680-mRNA-1 |
| chr4_3 | exon | 68863729 | 68863840 | 68863729 | ID=Msa0658680-mRNA-1:exon:20030;Parent=Msa0658680-mRNA-1 |
| chr4_3 | exon | 68863945 | 68864324 | 68863945 | ID=Msa0658680-mRNA-1:exon:20029;Parent=Msa0658680-mRNA-1 |
| chr4_3 | five_prime_UTR | 68863965 | 68864324 | 68863965 | ID=Msa0658680-mRNA-1:five_prime_utr;Parent=Msa0658680-mRNA-1 |
| chr4_3 | CDS | 68863945 | 68863964 | 68863945 | ID=Msa0658680-mRNA-1:cds;Parent=Msa0658680-mRNA-1 |
| chr4_3 | CDS | 68863729 | 68863840 | 68863729 | ID=Msa0658680-mRNA-1:cds;Parent=Msa0658680-mRNA-1 |
| chr4_3 | CDS | 68863487 | 68863615 | 68863487 | ID=Msa0658680-mRNA-1:cds;Parent=Msa0658680-mRNA-1 |
| chr4_3 | CDS | 68863023 | 68863127 | 68863023 | ID=Msa0658680-mRNA-1:cds;Parent=Msa0658680-mRNA-1 |
| chr4_3 | CDS | 68862638 | 68862721 | 68862638 | ID=Msa0658680-mRNA-1:cds;Parent=Msa0658680-mRNA-1 |
| chr4_3 | CDS | 68862468 | 68862557 | 68862468 | ID=Msa0658680-mRNA-1:cds;Parent=Msa0658680-mRNA-1 |
| chr4_3 | CDS | 68861912 | 68862024 | 68861912 | ID=Msa0658680-mRNA-1:cds;Parent=Msa0658680-mRNA-1 |
| chr4_3 | CDS | 68861683 | 68861806 | 68861683 | ID=Msa0658680-mRNA-1:cds;Parent=Msa0658680-mRNA-1 |
| chr4_3 | CDS | 68861448 | 68861501 | 68861448 | ID=Msa0658680-mRNA-1:cds;Parent=Msa0658680-mRNA-1 |
| chr4_3 | CDS | 68861285 | 68861359 | 68861285 | ID=Msa0658680-mRNA-1:cds;Parent=Msa0658680-mRNA-1 |
| chr4_3 | CDS | 68861157 | 68861192 | 68861157 | ID=Msa0658680-mRNA-1:cds;Parent=Msa0658680-mRNA-1 |
| chr4_3 | CDS | 68860885 | 68861001 | 68860885 | ID=Msa0658680-mRNA-1:cds;Parent=Msa0658680-mRNA-1 |
| chr4_3 | three_prime_UTR | 68860641 | 68860884 | 68860641 | ID=Msa0658680-mRNA-1:three_prime_utr;Parent=Msa0658680-mRNA-1 |
| Gene Sequence |
>Msa0658680 ATGAGGGGAGTTACTCACAGTTATAGTAAGAGAAAAACAAATGAAAGTATGAGGCTTGTTGTGATATTTTTCTTTGGAGGATTTCTTGGCTTATTTATAGGATTATCACTCCCAACATTCTCAACAACAAAGTTGAATCTGCCATCTAGCTTGCTTCCTTCCATTGATTTGTCATGCATTCGCGGTAGGGATACATGGTCTTTCATGAAGAATGATGATAGCATCTCATCACAAGACCAGTTAATGAATAATCCATCTAAGATTTGGGTTCCAACAAATCCTAGAGGTGCAGAAAGATTACCTCCTGGGATTGTTAATGCTCAATCAGACTTCTTCTTGCGCAGATTGTGGGGTCTTCCCAATGAGGATTTTACCTCTAAGCCAAAGTACCTTGTGACCTTCACTGTTGGGTATCAGCAGAAAAAGAATATTGATGCAGCTGCGAAAAAGTTTTCAAAGGACTTCACAATCCTTCTATTTCATTACGATGGCCGAACAACCGAATGGGATGAGTTTGAGTGGTCAAAAAAAGCTATCCATATACATAAAACTGGTGAAGAAGCATGGCTTGGAGATTTCCAGCCAGCTTTAGAACCTCAAAAAGGGTTGATTTGGAATATGACAAAGAGAAGAAATGACAGTGAAGTGCACAAACATGCACAAGAGAAACCAGGGAAGTGTAAATACCCTCTTCTTCCTCCTTGTGCAGCATTTGTCGAGATTATGGCTCCTGTTTTTTCGCGAGATGCATGGCGTTGTGTGTGGCACATGATTCAGAATGAATTTGTTCATGGATGGGGTCTTGATTTTGCTTTTAGAAAATGCGTCGAGCCTGCCCATGAGAAAATTGGAGTTGTTGATGCTCAATGGATTGTTCACCAAGGTATTCCATCACTAGGGGATCAGGGAGAAGCACAGACTGGGAAGCCGGCATGGCGGGCGGTTAAGGAGAGGTGTGGCATGGAATGGAGAATGTTCCAAGGTAGATTGACAAATGCAGAAAGGGGGTATTACAAGTCAAAGGGAATTGATTTTTCTGATTTGCTCGCTCATAACTAA |
| Protein sequence |
>Msa0658680 MRGVTHSYSKRKTNESMRLVVIFFFGGFLGLFIGLSLPTFSTTKLNLPSSLLPSIDLSCIRGRDTWSFMKNDDSISSQDQLMNNPSKIWVPTNPRGAERLPPGIVNAQSDFFLRRLWGLPNEDFTSKPKYLVTFTVGYQQKKNIDAAAKKFSKDFTILLFHYDGRTTEWDEFEWSKKAIHIHKTGEEAWLGDFQPALEPQKGLIWNMTKRRNDSEVHKHAQEKPGKCKYPLLPPCAAFVEIMAPVFSRDAWRCVWHMIQNEFVHGWGLDFAFRKCVEPAHEKIGVVDAQWIVHQGIPSLGDQGEAQTGKPAWRAVKERCGMEWRMFQGRLTNAERGYYKSKGIDFSDLLAHN* |
