AlfalfaGEDB | Msa0415140

M. sativa cultivar zhongmu-4 / Msa0415140

KEGG: K01520
GO: GO:0000287 GO:0003674 GO:0003824 GO:0004170 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006220 GO:0006221 GO:0006226 GO:0006244 GO:0006259 GO:0006281 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009129 GO:0009130 GO:0009141 GO:0009143 GO:0009147 GO:0009149 GO:0009157 GO:0009162 GO:0009165 GO:0009166 GO:0009176 GO:0009177 GO:0009200 GO:0009204 GO:0009211 GO:0009213 GO:0009219 GO:0009221 GO:0009223 GO:0009262 GO:0009263 GO:0009264 GO:0009265 GO:0009394 GO:0009987 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0018130 GO:0019438 GO:0019439 GO:0019637 GO:0019692 GO:0033554 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042802 GO:0043167 GO:0043169 GO:0043170 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046078 GO:0046080 GO:0046081 GO:0046385 GO:0046386 GO:0046434 GO:0046483 GO:0046700 GO:0046872 GO:0047429 GO:0050896 GO:0051716 GO:0055086 GO:0071704 GO:0072527 GO:0072528 GO:0072529 GO:0090304 GO:0090407 GO:1901135 GO:1901136 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576
PFAM: PF00692.22

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0415140	RHN67997.1	78.667	150	2	1	1	120	38	187	4.38e-72	223

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0415140	sp\|Q9STG6\|DUT_ARATH	88.785	107	12	0	14	120	60	166	1.40e-64	196

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0415140	A0A396IYG6	78.667	150	2	1	1	120	38	187	2.09e-72	223

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0015570	Msa0415140	0.801951	7.067342e-49	-8.615850e-47
Msa0016530	Msa0415140	0.800202	1.609532e-48	-8.615850e-47
Msa0020540	Msa0415140	0.807132	5.867977e-50	-8.615850e-47
Msa0025580	Msa0415140	0.828835	7.207787e-55	-8.615850e-47
Msa0032980	Msa0415140	0.838330	3.065714e-57	-8.615850e-47
Msa0050660	Msa0415140	0.800687	1.282097e-48	-8.615850e-47
Msa0050700	Msa0415140	0.800781	1.226869e-48	-8.615850e-47
Msa0053070	Msa0415140	0.801511	8.699749e-49	-8.615850e-47
Msa0062340	Msa0415140	0.821279	4.393393e-53	-8.615850e-47
Msa0067060	Msa0415140	0.809081	2.257145e-50	-8.615850e-47
Msa0067380	Msa0415140	0.802057	6.721042e-49	-8.615850e-47
Msa0075530	Msa0415140	0.813827	2.101199e-51	-8.615850e-47
Msa0078130	Msa0415140	0.802942	4.414608e-49	-8.615850e-47
Msa0091350	Msa0415140	0.850770	1.369993e-60	-8.615850e-47
Msa0091360	Msa0415140	0.808600	2.859793e-50	-8.615850e-47
Msa0093860	Msa0415140	0.820294	7.399960e-53	-8.615850e-47
Msa0098410	Msa0415140	0.814539	1.463430e-51	-8.615850e-47
Msa0103200	Msa0415140	0.803865	2.843299e-49	-8.615850e-47
Msa0106630	Msa0415140	0.820284	7.439751e-53	-8.615850e-47
Msa0115550	Msa0415140	0.813359	2.664161e-51	-8.615850e-47
Msa0123320	Msa0415140	0.806904	6.558044e-50	-8.615850e-47
Msa0125590	Msa0415140	0.813097	3.040672e-51	-8.615850e-47
Msa0127340	Msa0415140	0.824751	6.812902e-54	-8.615850e-47
Msa0127680	Msa0415140	0.813414	2.590750e-51	-8.615850e-47
Msa0140200	Msa0415140	0.813631	2.321142e-51	-8.615850e-47
Msa0140380	Msa0415140	0.833420	5.387604e-56	-8.615850e-47
Msa0141640	Msa0415140	0.802840	4.634740e-49	-8.615850e-47
Msa0153210	Msa0415140	0.822227	2.651368e-53	-8.615850e-47
Msa0156090	Msa0415140	0.800874	1.174151e-48	-8.615850e-47
Msa0159590	Msa0415140	0.811411	7.096514e-51	-8.615850e-47
Msa0160160	Msa0415140	0.814474	1.512888e-51	-8.615850e-47
Msa0160170	Msa0415140	0.821078	4.888886e-53	-8.615850e-47
Msa0160290	Msa0415140	0.803672	3.116883e-49	-8.615850e-47
Msa0165830	Msa0415140	0.835380	1.735703e-56	-8.615850e-47
Msa0166170	Msa0415140	0.861966	7.076574e-64	-8.615850e-47
Msa0168050	Msa0415140	0.810799	9.632411e-51	-8.615850e-47
Msa0232760	Msa0415140	0.803739	3.019885e-49	-8.615850e-47
Msa0367520	Msa0415140	0.848307	6.662151e-60	-8.615850e-47
Msa0368860	Msa0415140	0.803310	3.705625e-49	-8.615850e-47
Msa0373170	Msa0415140	0.837167	6.096523e-57	-8.615850e-47
Msa0373500	Msa0415140	0.813347	2.680025e-51	-8.615850e-47
Msa0380670	Msa0415140	0.800332	1.514699e-48	-8.615850e-47
Msa0382740	Msa0415140	0.822471	2.327151e-53	-8.615850e-47
Msa0382770	Msa0415140	0.825417	4.742357e-54	-8.615850e-47
Msa0383000	Msa0415140	0.822761	1.992954e-53	-8.615850e-47
Msa0400950	Msa0415140	0.800321	1.522331e-48	-8.615850e-47
Msa0412130	Msa0415140	0.818675	1.731628e-52	-8.615850e-47
Msa0415140	Msa0415820	0.820118	8.118985e-53	-8.615850e-47
Msa0415140	Msa0416160	0.820653	6.121880e-53	-8.615850e-47
Msa0415140	Msa0420670	0.852041	5.988677e-61	-8.615850e-47
Msa0415140	Msa0421130	0.835870	1.304727e-56	-8.615850e-47
Msa0415140	Msa0421750	0.831688	1.448380e-55	-8.615850e-47
Msa0415140	Msa0430480	0.814772	1.299602e-51	-8.615850e-47
Msa0415140	Msa0432010	0.827064	1.923217e-54	-8.615850e-47
Msa0415140	Msa0433150	0.820522	6.559739e-53	-8.615850e-47
Msa0415140	Msa0433760	0.828220	1.014840e-54	-8.615850e-47
Msa0415140	Msa0443410	0.854823	9.523402e-62	-8.615850e-47
Msa0415140	Msa0462780	0.801661	8.105179e-49	-8.615850e-47
Msa0415140	Msa0466520	0.845998	2.861969e-59	-8.615850e-47
Msa0415140	Msa0466880	0.850106	2.103916e-60	-8.615850e-47
Msa0415140	Msa0467690	0.839232	1.792524e-57	-8.615850e-47
Msa0415140	Msa0468620	0.804733	1.874447e-49	-8.615850e-47
Msa0415140	Msa0476280	0.815015	1.148089e-51	-8.615850e-47
Msa0415140	Msa0477630	0.831555	1.562141e-55	-8.615850e-47
Msa0415140	Msa0480730	0.803762	2.985866e-49	-8.615850e-47
Msa0415140	Msa0481890	0.806511	7.939551e-50	-8.615850e-47
Msa0415140	Msa0506880	0.848359	6.444903e-60	-8.615850e-47
Msa0415140	Msa0507810	0.807262	5.506910e-50	-8.615850e-47
Msa0415140	Msa0507920	0.818762	1.654588e-52	-8.615850e-47
Msa0415140	Msa0512530	0.843491	1.356290e-58	-8.615850e-47
Msa0415140	Msa0513080	0.833580	4.915109e-56	-8.615850e-47
Msa0415140	Msa0513560	0.843190	1.631494e-58	-8.615850e-47
Msa0415140	Msa0514020	0.835050	2.102487e-56	-8.615850e-47
Msa0415140	Msa0514500	0.804973	1.670472e-49	-8.615850e-47
Msa0415140	Msa0520870	0.842730	2.163314e-58	-8.615850e-47
Msa0415140	Msa0521680	0.833614	4.820441e-56	-8.615850e-47
Msa0415140	Msa0524520	0.824688	7.048715e-54	-8.615850e-47
Msa0415140	Msa0524800	0.821520	3.865490e-53	-8.615850e-47
Msa0415140	Msa0525000	0.822447	2.357158e-53	-8.615850e-47
Msa0415140	Msa0527850	0.819577	1.079746e-52	-8.615850e-47
Msa0415140	Msa0534100	0.821342	4.249197e-53	-8.615850e-47
Msa0415140	Msa0537990	0.812359	4.412181e-51	-8.615850e-47
Msa0415140	Msa0548000	0.814921	1.204411e-51	-8.615850e-47
Msa0415140	Msa0550590	0.845946	2.956088e-59	-8.615850e-47
Msa0415140	Msa0554300	0.825209	5.311186e-54	-8.615850e-47
Msa0415140	Msa0559100	0.804224	2.394237e-49	-8.615850e-47
Msa0415140	Msa0561560	0.873994	9.548534e-68	-8.615850e-47
Msa0415140	Msa0563740	0.864934	8.504396e-65	-8.615850e-47
Msa0415140	Msa0563980	0.836816	7.497834e-57	-8.615850e-47
Msa0415140	Msa0577280	0.816510	5.328192e-52	-8.615850e-47
Msa0415140	Msa0577330	0.816909	4.336239e-52	-8.615850e-47
Msa0415140	Msa0580200	0.817314	3.516407e-52	-8.615850e-47
Msa0415140	Msa0582460	0.800870	1.176505e-48	-8.615850e-47
Msa0415140	Msa0586200	0.804903	1.727809e-49	-8.615850e-47
Msa0415140	Msa0590200	0.813471	2.517465e-51	-8.615850e-47
Msa0415140	Msa0603980	0.802075	6.662153e-49	-8.615850e-47
Msa0415140	Msa0606100	0.879129	1.602820e-69	-8.615850e-47
Msa0415140	Msa0607800	0.809383	1.944049e-50	-8.615850e-47
Msa0415140	Msa0608350	0.817324	3.498180e-52	-8.615850e-47
Msa0415140	Msa0613580	0.803099	4.096851e-49	-8.615850e-47
Msa0415140	Msa0613630	0.816954	4.236839e-52	-8.615850e-47
Msa0415140	Msa0615630	0.844281	8.332009e-59	-8.615850e-47
Msa0415140	Msa0620500	0.860368	2.169133e-63	-8.615850e-47
Msa0415140	Msa0621710	0.850078	2.142407e-60	-8.615850e-47
Msa0415140	Msa0622920	0.811270	7.614727e-51	-8.615850e-47
Msa0415140	Msa0625620	0.817889	2.609385e-52	-8.615850e-47
Msa0415140	Msa0630660	0.804431	2.167355e-49	-8.615850e-47
Msa0415140	Msa0645340	0.821796	3.336331e-53	-8.615850e-47
Msa0415140	Msa0647050	0.867195	1.636549e-65	-8.615850e-47
Msa0415140	Msa0647440	0.824123	9.570765e-54	-8.615850e-47
Msa0415140	Msa0648040	0.811549	6.622230e-51	-8.615850e-47
Msa0415140	Msa0648760	0.831764	1.387642e-55	-8.615850e-47
Msa0415140	Msa0654740	0.856652	2.783669e-62	-8.615850e-47
Msa0415140	Msa0668900	0.838896	2.189249e-57	-8.615850e-47
Msa0415140	Msa0687090	0.823764	1.162140e-53	-8.615850e-47
Msa0415140	Msa0690320	0.811250	7.688944e-51	-8.615850e-47
Msa0415140	Msa0690740	0.814271	1.677073e-51	-8.615850e-47
Msa0415140	Msa0691270	0.841067	5.948998e-58	-8.615850e-47
Msa0415140	Msa0698730	0.827635	1.402772e-54	-8.615850e-47
Msa0415140	Msa0700500	0.804080	2.564943e-49	-8.615850e-47
Msa0415140	Msa0709440	0.804275	2.335977e-49	-8.615850e-47
Msa0415140	Msa0714520	0.832041	1.185322e-55	-8.615850e-47
Msa0415140	Msa0717830	0.809612	1.736054e-50	-8.615850e-47
Msa0415140	Msa0717900	0.832765	7.841439e-56	-8.615850e-47
Msa0415140	Msa0718040	0.814836	1.257868e-51	-8.615850e-47
Msa0415140	Msa0720280	0.812606	3.895560e-51	-8.615850e-47
Msa0415140	Msa0722220	0.806695	7.260318e-50	-8.615850e-47
Msa0415140	Msa0743440	0.806150	9.458646e-50	-8.615850e-47
Msa0415140	Msa0745720	0.801246	9.857698e-49	-8.615850e-47
Msa0415140	Msa0747740	0.824515	7.740694e-54	-8.615850e-47
Msa0415140	Msa0758590	0.800705	1.271507e-48	-8.615850e-47
Msa0415140	Msa0773630	0.800071	1.711941e-48	-8.615850e-47
Msa0415140	Msa0780910	0.811198	7.892679e-51	-8.615850e-47
Msa0415140	Msa0800660	0.846520	2.062332e-59	-8.615850e-47
Msa0415140	Msa0803140	0.807160	5.790431e-50	-8.615850e-47
Msa0415140	Msa0805680	0.816549	5.223537e-52	-8.615850e-47
Msa0415140	Msa0811020	0.820807	5.642237e-53	-8.615850e-47
Msa0415140	Msa0816080	0.814643	1.388126e-51	-8.615850e-47
Msa0415140	Msa0818880	0.805105	1.567160e-49	-8.615850e-47
Msa0415140	Msa0821160	0.809700	1.662332e-50	-8.615850e-47
Msa0415140	Msa0841500	0.804997	1.650832e-49	-8.615850e-47
Msa0415140	Msa0863620	0.802533	5.361992e-49	-8.615850e-47
Msa0415140	Msa0879920	0.816077	6.661070e-52	-8.615850e-47
Msa0415140	Msa0909760	0.858279	9.186125e-63	-8.615850e-47
Msa0415140	Msa0924430	0.821609	3.686220e-53	-8.615850e-47
Msa0415140	Msa0931590	0.848380	6.361305e-60	-8.615850e-47
Msa0415140	Msa0966710	0.813931	1.994050e-51	-8.615850e-47
Msa0415140	Msa1000970	0.808304	3.307118e-50	-8.615850e-47
Msa0415140	Msa1008670	0.807739	4.363961e-50	-8.615850e-47
Msa0415140	Msa1008680	0.839870	1.223642e-57	-8.615850e-47
Msa0415140	Msa1033920	0.850041	2.193431e-60	-8.615850e-47
Msa0415140	Msa1035570	0.849628	2.861245e-60	-8.615850e-47
Msa0415140	Msa1035610	0.841618	4.259106e-58	-8.615850e-47
Msa0415140	Msa1042880	0.802868	4.572907e-49	-8.615850e-47
Msa0415140	Msa1060900	0.809208	2.120003e-50	-8.615850e-47
Msa0415140	Msa1076020	0.822132	2.789708e-53	-8.615850e-47
Msa0415140	Msa1078880	0.826040	3.374620e-54	-8.615850e-47
Msa0415140	Msa1078970	0.819471	1.141177e-52	-8.615850e-47
Msa0415140	Msa1079640	0.802374	5.783102e-49	-8.615850e-47
Msa0415140	Msa1081390	0.801291	9.650250e-49	-8.615850e-47
Msa0415140	Msa1082380	0.845099	5.016122e-59	-8.615850e-47
Msa0415140	Msa1084530	0.813310	2.731298e-51	-8.615850e-47
Msa0415140	Msa1104220	0.809184	2.144854e-50	-8.615850e-47
Msa0415140	Msa1106220	0.800432	1.445403e-48	-8.615850e-47
Msa0415140	Msa1116190	0.811738	6.024892e-51	-8.615850e-47
Msa0415140	Msa1116220	0.811743	6.009826e-51	-8.615850e-47
Msa0415140	Msa1116660	0.866718	2.323119e-65	-8.615850e-47
Msa0415140	Msa1116740	0.858166	9.927921e-63	-8.615850e-47
Msa0415140	Msa1125820	0.812430	4.257335e-51	-8.615850e-47
Msa0415140	Msa1125990	0.809014	2.332865e-50	-8.615850e-47
Msa0415140	Msa1127280	0.848812	4.829451e-60	-8.615850e-47
Msa0415140	Msa1130420	0.802025	6.822484e-49	-8.615850e-47
Msa0415140	Msa1132530	0.817408	3.349360e-52	-8.615850e-47
Msa0415140	Msa1134790	0.801012	1.100509e-48	-8.615850e-47
Msa0415140	Msa1139050	0.837309	5.608600e-57	-8.615850e-47
Msa0415140	Msa1148630	0.802582	5.239749e-49	-8.615850e-47
Msa0415140	Msa1149450	0.807206	5.660188e-50	-8.615850e-47
Msa0415140	Msa1159590	0.808664	2.771730e-50	-8.615850e-47
Msa0415140	Msa1162880	0.873682	1.216606e-67	-8.615850e-47
Msa0415140	Msa1163180	0.812005	5.270375e-51	-8.615850e-47
Msa0415140	Msa1165480	0.852470	4.519750e-61	-8.615850e-47
Msa0415140	Msa1169190	0.825196	5.349120e-54	-8.615850e-47
Msa0415140	Msa1190390	0.814122	1.809528e-51	-8.615850e-47
Msa0415140	Msa1213830	0.808382	3.183657e-50	-8.615850e-47
Msa0415140	Msa1214350	0.824163	9.367195e-54	-8.615850e-47
Msa0415140	Msa1215980	0.860606	1.837138e-63	-8.615850e-47
Msa0415140	Msa1218820	0.809030	2.314376e-50	-8.615850e-47
Msa0415140	Msa1220170	0.817200	3.731356e-52	-8.615850e-47
Msa0415140	Msa1223390	0.836442	9.338342e-57	-8.615850e-47
Msa0415140	Msa1244240	0.809305	2.020769e-50	-8.615850e-47
Msa0415140	Msa1254420	0.824506	7.777493e-54	-8.615850e-47
Msa0415140	Msa1254540	0.851629	7.835625e-61	-8.615850e-47
Msa0415140	Msa1261050	0.827243	1.742193e-54	-8.615850e-47
Msa0415140	Msa1263740	0.855353	6.676565e-62	-8.615850e-47
Msa0415140	Msa1263780	0.837639	4.616570e-57	-8.615850e-47
Msa0415140	Msa1266300	0.805220	1.482990e-49	-8.615850e-47
Msa0415140	Msa1294460	0.813057	3.103702e-51	-8.615850e-47
Msa0415140	Msa1294790	0.831386	1.719005e-55	-8.615850e-47
Msa0415140	Msa1299980	0.805411	1.352392e-49	-8.615850e-47
Msa0415140	Msa1300460	0.826227	3.045282e-54	-8.615850e-47
Msa0415140	Msa1301820	0.820348	7.194528e-53	-8.615850e-47
Msa0415140	Msa1305780	0.805199	1.498130e-49	-8.615850e-47
Msa0415140	Msa1321530	0.802371	5.790488e-49	-8.615850e-47
Msa0415140	Msa1332950	0.836804	7.547183e-57	-8.615850e-47
Msa0415140	Msa1339170	0.854748	1.001395e-61	-8.615850e-47
Msa0415140	Msa1342810	0.827270	1.716447e-54	-8.615850e-47
Msa0415140	Msa1359980	0.805896	1.069856e-49	-8.615850e-47
Msa0415140	Msa1361930	0.830046	3.660595e-55	-8.615850e-47
Msa0415140	Msa1364410	0.833031	6.735789e-56	-8.615850e-47
Msa0415140	Msa1368750	0.831301	1.804290e-55	-8.615850e-47
Msa0415140	Msa1374790	0.837612	4.689578e-57	-8.615850e-47
Msa0415140	Msa1375350	0.802848	4.618357e-49	-8.615850e-47
Msa0415140	Msa1381470	0.820967	5.185521e-53	-8.615850e-47
Msa0415140	Msa1393560	0.814746	1.316926e-51	-8.615850e-47
Msa0415140	Msa1400010	0.800800	1.215874e-48	-8.615850e-47
Msa0415140	Msa1406720	0.845898	3.047745e-59	-8.615850e-47
Msa0415140	Msa1409410	0.805771	1.136494e-49	-8.615850e-47
Msa0415140	Msa1426320	0.802773	4.783808e-49	-8.615850e-47
Msa0415140	Msa1434640	0.859185	4.925404e-63	-8.615850e-47
Msa0415140	Msa1442260	0.811504	6.773477e-51	-8.615850e-47
Msa0415140	Msa1446100	0.804665	1.936664e-49	-8.615850e-47
Msa0415140	Msa1447170	0.815936	7.161390e-52	-8.615850e-47
Msa0415140	Msa1450190	0.808436	3.100229e-50	-8.615850e-47
Msa0415140	Msa1451820	0.836686	8.089689e-57	-8.615850e-47
Msa0415140	Msa1457840	0.804513	2.083537e-49	-8.615850e-47
Msa0341680	Msa0415140	0.809669	1.688231e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0415140	MtrunA17_Chr3g0108931	78.667	150	2	1	1	120	38	187	4.03e-76	223

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0415140	AT3G46940.1	88.785	107	12	0	14	120	60	166	1.42e-65	196
Msa0415140	AT3G46940.2	88.785	107	12	0	14	120	101	207	1.97e-65	197

Find 32 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCGGAAGTTTCTGAGGTTT+TGG	0.225618	3_2:+61213208	Msa0415140:CDS
GTTAGAGGTGAAGGTGGTTT+TGG	0.293840	3_2:+61213245	Msa0415140:CDS
GATTTGGATTCTACTGTTAG+AGG	0.343621	3_2:+61213230	Msa0415140:CDS
TTTCTTCACTCGGAGAAAAC+TGG	0.351836	3_2:-61212674	None:intergenic
ACACGAGCATAGGTTCCTTC+AGG	0.369361	3_2:-61212929	None:intergenic
TCAACAATGTCCAACTTAAA+CGG	0.388632	3_2:+61212640	None:intergenic
CTGATTACAGAGGACCTGTT+GGG	0.415838	3_2:+61213099	Msa0415140:CDS
CATTCGATTGACGTTGGTGC+TGG	0.432456	3_2:+61213065	Msa0415140:CDS
TCCAAAACCTCAGAAACTTC+CGG	0.440429	3_2:-61213209	None:intergenic
ATGAGAGCTTTGCCGCGAGC+AGG	0.461473	3_2:-61212881	None:intergenic
TGAAACGACGCCGTTTAAGT+TGG	0.465891	3_2:-61212650	None:intergenic
CGCCGAGATCGGGGTTGACA+TGG	0.473074	3_2:+61213039	Msa0415140:CDS
TTCCATGTCAACCCCGATCT+CGG	0.481839	3_2:-61213041	None:intergenic
TTAAACAGTATAACCCCAAC+AGG	0.497604	3_2:-61213113	None:intergenic
GATTCTACTGTTAGAGGTGA+AGG	0.505946	3_2:+61213236	Msa0415140:CDS
CTCTCCATCGCCGTCCCTGA+AGG	0.513836	3_2:+61212914	Msa0415140:CDS
CACGAGCATAGGTTCCTTCA+GGG	0.535140	3_2:-61212928	None:intergenic
TATTGAGAAGATTATTACTC+CGG	0.540579	3_2:+61213190	Msa0415140:CDS
ACGGCGATGGAGAGATCTGT+GGG	0.554559	3_2:-61212905	None:intergenic
GGTTCCTTCAGGGACGGCGA+TGG	0.559917	3_2:-61212918	None:intergenic
TATTACTCCGGAAGTTTCTG+AGG	0.562326	3_2:+61213202	Msa0415140:CDS
TCTACTGTTAGAGGTGAAGG+TGG	0.578300	3_2:+61213239	Msa0415140:CDS
GACGGCGATGGAGAGATCTG+TGG	0.578622	3_2:-61212906	None:intergenic
GTAATTACCAACACGAGCAT+AGG	0.588762	3_2:-61212939	None:intergenic
GAAGGAACCTATGCTCGTGT+TGG	0.606547	3_2:+61212932	Msa0415140:CDS
GCTGATTACAGAGGACCTGT+TGG	0.614644	3_2:+61213098	Msa0415140:CDS
TGGAAGCATTCGATTGACGT+TGG	0.614719	3_2:+61213059	Msa0415140:CDS
AGCATAGGTTCCTTCAGGGA+CGG	0.629807	3_2:-61212924	None:intergenic
GATTCAAAGGTTCCTGCTCG+CGG	0.632457	3_2:+61212869	Msa0415140:intron
TGATTACAGAGGACCTGTTG+GGG	0.654205	3_2:+61213100	Msa0415140:CDS
GTGATTGATGCTGATTACAG+AGG	0.740502	3_2:+61213089	Msa0415140:CDS
CGGCGATGGAGAGATCTGTG+GGG	0.756952	3_2:-61212904	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAAATTCAAAATTGGGAAA+GGG	-	chr3_2:61212972-61212991	None:intergenic	20.0%
!!!	TTTCCCAATTTTGAATTTTA+GGG	+	chr3_2:61212972-61212991	Msa0415140:intron	20.0%
!!	AAACCCTAAAATTCAAAATT+GGG	-	chr3_2:61212978-61212997	None:intergenic	20.0%
!!	AAAACCCTAAAATTCAAAAT+TGG	-	chr3_2:61212979-61212998	None:intergenic	20.0%
!!!	ATTTTAGGGTTTTGAAATTT+AGG	+	chr3_2:61212986-61213005	Msa0415140:intron	20.0%
!!!	TTTTAGGGTTTTGAAATTTA+GGG	+	chr3_2:61212987-61213006	Msa0415140:intron	20.0%
!	AAAATTCAAAATTGGGAAAG+GGG	-	chr3_2:61212971-61212990	None:intergenic	25.0%
!	CTAAAATTCAAAATTGGGAA+AGG	-	chr3_2:61212973-61212992	None:intergenic	25.0%
!!	CTTTCCCAATTTTGAATTTT+AGG	+	chr3_2:61212971-61212990	Msa0415140:intron	25.0%
!	TATTGAGAAGATTATTACTC+CGG	+	chr3_2:61213190-61213209	Msa0415140:CDS	25.0%
!!!	GTTGATTTTGAAGTGAAAGT+TGG	+	chr3_2:61213146-61213165	Msa0415140:CDS	30.0%
!!	NNNNNNNNCCGCCGATTCAA+AGG	+	chr3_2:61212856-61212875	Msa0415140:intron	35.0%
	TTAAACAGTATAACCCCAAC+AGG	-	chr3_2:61213116-61213135	None:intergenic	35.0%
!!!	AGTTTCTGAGGTTTTGGATT+TGG	+	chr3_2:61213214-61213233	Msa0415140:CDS	35.0%
	GATTTGGATTCTACTGTTAG+AGG	+	chr3_2:61213230-61213249	Msa0415140:CDS	35.0%
	TTTCTTCACTCGGAGAAAAC+TGG	-	chr3_2:61212677-61212696	None:intergenic	40.0%
	GTAATTACCAACACGAGCAT+AGG	-	chr3_2:61212942-61212961	None:intergenic	40.0%
!	GTGATTGATGCTGATTACAG+AGG	+	chr3_2:61213089-61213108	Msa0415140:CDS	40.0%
	TATTACTCCGGAAGTTTCTG+AGG	+	chr3_2:61213202-61213221	Msa0415140:CDS	40.0%
	TCCAAAACCTCAGAAACTTC+CGG	-	chr3_2:61213212-61213231	None:intergenic	40.0%
	GATTCTACTGTTAGAGGTGA+AGG	+	chr3_2:61213236-61213255	Msa0415140:CDS	40.0%
!!!	AAGGTGGTTTTGGATCTACT+GGG	+	chr3_2:61213255-61213274	Msa0415140:CDS	40.0%
	TGAAACGACGCCGTTTAAGT+TGG	-	chr3_2:61212653-61212672	None:intergenic	45.0%
	TGTCGGAGAGTTTCTTCACT+CGG	-	chr3_2:61212687-61212706	None:intergenic	45.0%
!	GGAAGGTATAACGGCTTTGT+CGG	-	chr3_2:61212704-61212723	None:intergenic	45.0%
!!	TGGAAGCATTCGATTGACGT+TGG	+	chr3_2:61213059-61213078	Msa0415140:CDS	45.0%
	CTGATTACAGAGGACCTGTT+GGG	+	chr3_2:61213099-61213118	Msa0415140:CDS	45.0%
	TGATTACAGAGGACCTGTTG+GGG	+	chr3_2:61213100-61213119	Msa0415140:CDS	45.0%
!!!	TCCGGAAGTTTCTGAGGTTT+TGG	+	chr3_2:61213208-61213227	Msa0415140:CDS	45.0%
	TCTACTGTTAGAGGTGAAGG+TGG	+	chr3_2:61213239-61213258	Msa0415140:CDS	45.0%
!	GTTAGAGGTGAAGGTGGTTT+TGG	+	chr3_2:61213245-61213264	Msa0415140:CDS	45.0%
!!!	GAAGGTGGTTTTGGATCTAC+TGG	+	chr3_2:61213254-61213273	Msa0415140:CDS	45.0%
!!!	AGGTGGTTTTGGATCTACTG+GGG	+	chr3_2:61213256-61213275	Msa0415140:CDS	45.0%
	AAAGCCGTTATACCTTCCCG+CGG	+	chr3_2:61212706-61212725	Msa0415140:intron	50.0%
	AGCAGGAACCTTTGAATCGG+CGG	-	chr3_2:61212867-61212886	None:intergenic	50.0%
	GCGAGCAGGAACCTTTGAAT+CGG	-	chr3_2:61212870-61212889	None:intergenic	50.0%
	GATTCAAAGGTTCCTGCTCG+CGG	+	chr3_2:61212869-61212888	Msa0415140:intron	50.0%
	AGCATAGGTTCCTTCAGGGA+CGG	-	chr3_2:61212927-61212946	None:intergenic	50.0%
	CACGAGCATAGGTTCCTTCA+GGG	-	chr3_2:61212931-61212950	None:intergenic	50.0%
	ACACGAGCATAGGTTCCTTC+AGG	-	chr3_2:61212932-61212951	None:intergenic	50.0%
	GAAGGAACCTATGCTCGTGT+TGG	+	chr3_2:61212932-61212951	Msa0415140:CDS	50.0%
!!	GATTTTTGCAGCGCCGAGAT+CGG	+	chr3_2:61213028-61213047	Msa0415140:intron	50.0%
!!	ATTTTTGCAGCGCCGAGATC+GGG	+	chr3_2:61213029-61213048	Msa0415140:intron	50.0%
	TTCCATGTCAACCCCGATCT+CGG	-	chr3_2:61213044-61213063	None:intergenic	50.0%
	CATTCGATTGACGTTGGTGC+TGG	+	chr3_2:61213065-61213084	Msa0415140:CDS	50.0%
	GCTGATTACAGAGGACCTGT+TGG	+	chr3_2:61213098-61213117	Msa0415140:CDS	50.0%
!	AGAGCCGCGGGAAGGTATAA+CGG	-	chr3_2:61212713-61212732	None:intergenic	55.0%
	ACGGCGATGGAGAGATCTGT+GGG	-	chr3_2:61212908-61212927	None:intergenic	55.0%
!!	TTTTTGCAGCGCCGAGATCG+GGG	+	chr3_2:61213030-61213049	Msa0415140:intron	55.0%
	AGATCGTATCCGGCGGAGAG+AGG	-	chr3_2:61212736-61212755	None:intergenic	60.0%
	GCTGGAGAGATCGTATCCGG+CGG	-	chr3_2:61212743-61212762	None:intergenic	60.0%
	ATGAGAGCTTTGCCGCGAGC+AGG	-	chr3_2:61212884-61212903	None:intergenic	60.0%
	CGGCGATGGAGAGATCTGTG+GGG	-	chr3_2:61212907-61212926	None:intergenic	60.0%
	GACGGCGATGGAGAGATCTG+TGG	-	chr3_2:61212909-61212928	None:intergenic	60.0%
	CTCTCCATCGCCGTCCCTGA+AGG	+	chr3_2:61212914-61212933	Msa0415140:CDS	65.0%
	GGTTCCTTCAGGGACGGCGA+TGG	-	chr3_2:61212921-61212940	None:intergenic	65.0%
	CGCCGAGATCGGGGTTGACA+TGG	+	chr3_2:61213039-61213058	Msa0415140:CDS	65.0%
	GAGAGAGGAGAGCCGCGGGA+AGG	-	chr3_2:61212721-61212740	None:intergenic	70.0%
	GGCGGAGAGAGGAGAGCCGC+GGG	-	chr3_2:61212725-61212744	None:intergenic	75.0%
	CGGCGGAGAGAGGAGAGCCG+CGG	-	chr3_2:61212726-61212745	None:intergenic	75.0%
	CGCGGCTCTCCTCTCTCCGC+CGG	+	chr3_2:61212724-61212743	Msa0415140:intron	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	61212646	61213283	61212646	ID=Msa0415140;Name=Msa0415140
chr3_2	mRNA	61212646	61213283	61212646	ID=Msa0415140-mRNA-1;Parent=Msa0415140;Name=Msa0415140-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0\|0\|0\|1\|1\|1\|3\|0\|120
chr3_2	exon	61212646	61212687	61212646	ID=Msa0415140-mRNA-1:exon:12927;Parent=Msa0415140-mRNA-1
chr3_2	exon	61212878	61212953	61212878	ID=Msa0415140-mRNA-1:exon:12928;Parent=Msa0415140-mRNA-1
chr3_2	exon	61213039	61213283	61213039	ID=Msa0415140-mRNA-1:exon:12929;Parent=Msa0415140-mRNA-1
chr3_2	CDS	61212646	61212687	61212646	ID=Msa0415140-mRNA-1:cds;Parent=Msa0415140-mRNA-1
chr3_2	CDS	61212878	61212953	61212878	ID=Msa0415140-mRNA-1:cds;Parent=Msa0415140-mRNA-1
chr3_2	CDS	61213039	61213283	61213039	ID=Msa0415140-mRNA-1:cds;Parent=Msa0415140-mRNA-1

Gene Sequence	>Msa0415140 ATGTCCAACTTAAACGGCGTCGTTTCATCCAGTTTTCTCCGAGTTCCTGCTCGCGGCAAAGCTCTCATCCCCACAGATCTCTCCATCGCCGTCCCTGAAGGAACCTATGCTCGTGTTGCGCCGAGATCGGGGTTGACATGGAAGCATTCGATTGACGTTGGTGCTGGAGTGATTGATGCTGATTACAGAGGACCTGTTGGGGTTATACTGTTTAATCATTCAGATGTTGATTTTGAAGTGAAAGTTGGTGATAGAGTTGCTCAGTTGATTATTGAGAAGATTATTACTCCGGAAGTTTCTGAGGTTTTGGATTTGGATTCTACTGTTAGAGGTGAAGGTGGTTTTGGATCTACTGGGGTTTGA
Protein sequence	>Msa0415140 MSNLNGVVSSSFLRVPARGKALIPTDLSIAVPEGTYARVAPRSGLTWKHSIDVGAGVIDADYRGPVGVILFNHSDVDFEVKVGDRVAQLIIEKIITPEVSEVLDLDSTVRGEGGFGSTGV*