AlfalfaGEDB | Msa0622960

M. sativa cultivar zhongmu-4 / Msa0622960

KEGG: K12524
GO: GO:0003674 GO:0003824 GO:0004072 GO:0004412 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009069 GO:0009070 GO:0009090 GO:0009092 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016301 GO:0016310 GO:0016491 GO:0016614 GO:0016616 GO:0016740 GO:0016772 GO:0016774 GO:0019202 GO:0019752 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0055114 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0622960	PNY10347.1	84.337	83	13	0	22	104	87	169	1.93e-41	147

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0622960	sp\|P37142\|AKH_DAUCA	65.823	79	27	0	24	102	776	854	3.74e-29	113

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0002490	Msa0622960	0.804729	1.878242e-49	-8.615850e-47
Msa0030380	Msa0622960	0.801995	6.920783e-49	-8.615850e-47
Msa0032260	Msa0622960	0.806049	9.934159e-50	-8.615850e-47
Msa0032840	Msa0622960	-0.813510	2.468212e-51	-8.615850e-47
Msa0056470	Msa0622960	0.810782	9.714239e-51	-8.615850e-47
Msa0064350	Msa0622960	0.818007	2.453961e-52	-8.615850e-47
Msa0064380	Msa0622960	0.804513	2.083513e-49	-8.615850e-47
Msa0072810	Msa0622960	0.807730	4.382736e-50	-8.615850e-47
Msa0073670	Msa0622960	0.835723	1.420916e-56	-8.615850e-47
Msa0082280	Msa0622960	0.837570	4.808732e-57	-8.615850e-47
Msa0622960	Msa0644860	0.814897	1.219057e-51	-8.615850e-47
Msa0622960	Msa0661300	0.810751	9.863447e-51	-8.615850e-47
Msa0622960	Msa0677290	0.812878	3.396386e-51	-8.615850e-47
Msa0622960	Msa0687630	0.854341	1.313334e-61	-8.615850e-47
Msa0622960	Msa0692080	0.811023	8.612048e-51	-8.615850e-47
Msa0622960	Msa0692840	0.803462	3.446968e-49	-8.615850e-47
Msa0622960	Msa0692940	0.819001	1.460315e-52	-8.615850e-47
Msa0622960	Msa0696620	0.829773	4.267123e-55	-8.615850e-47
Msa0622960	Msa0718740	0.813291	2.756972e-51	-8.615850e-47
Msa0622960	Msa0763020	0.800248	1.575755e-48	-8.615850e-47
Msa0622960	Msa0763070	0.809208	2.119498e-50	-8.615850e-47
Msa0622960	Msa0787750	0.818090	2.349819e-52	-8.615850e-47
Msa0622960	Msa0823090	0.802582	5.239879e-49	-8.615850e-47
Msa0622960	Msa0824640	0.801078	1.066961e-48	-8.615850e-47
Msa0622960	Msa0837660	0.808675	2.756113e-50	-8.615850e-47
Msa0622960	Msa0858770	0.812651	3.807572e-51	-8.615850e-47
Msa0622960	Msa0864010	0.800565	1.357821e-48	-8.615850e-47
Msa0622960	Msa0937550	0.805407	1.355296e-49	-8.615850e-47
Msa0622960	Msa0966270	0.806605	7.586162e-50	-8.615850e-47
Msa0622960	Msa0968590	0.820832	5.567342e-53	-8.615850e-47
Msa0622960	Msa1019240	0.805775	1.134479e-49	-8.615850e-47
Msa0622960	Msa1019260	0.821467	3.975232e-53	-8.615850e-47
Msa0622960	Msa1040830	0.800008	1.762800e-48	-8.615850e-47
Msa0622960	Msa1081150	0.822143	2.772749e-53	-8.615850e-47
Msa0622960	Msa1082700	0.839585	1.451098e-57	-8.615850e-47
Msa0622960	Msa1083150	0.822064	2.893325e-53	-8.615850e-47
Msa0622960	Msa1104140	0.810819	9.536077e-51	-8.615850e-47
Msa0622960	Msa1104210	0.807981	3.874987e-50	-8.615850e-47
Msa0622960	Msa1115770	0.806750	7.067401e-50	-8.615850e-47
Msa0622960	Msa1126510	0.859536	3.866141e-63	-8.615850e-47
Msa0622960	Msa1126770	0.829647	4.578517e-55	-8.615850e-47
Msa0622960	Msa1127890	0.824119	9.592653e-54	-8.615850e-47
Msa0622960	Msa1134110	0.837491	5.037458e-57	-8.615850e-47
Msa0622960	Msa1165870	0.853638	2.093326e-61	-8.615850e-47
Msa0622960	Msa1165940	0.812618	3.872124e-51	-8.615850e-47
Msa0622960	Msa1166860	0.863113	3.138009e-64	-8.615850e-47
Msa0622960	Msa1210810	0.800370	1.488232e-48	-8.615850e-47
Msa0622960	Msa1249870	0.811412	7.093519e-51	-8.615850e-47
Msa0622960	Msa1292830	0.803712	3.058057e-49	-8.615850e-47
Msa0622960	Msa1304820	0.808318	3.284790e-50	-8.615850e-47
Msa0622960	Msa1307950	0.823350	1.452119e-53	-8.615850e-47
Msa0622960	Msa1321630	0.813218	2.861507e-51	-8.615850e-47
Msa0622960	Msa1335940	0.809899	1.506273e-50	-8.615850e-47
Msa0622960	Msa1367880	0.803814	2.912240e-49	-8.615850e-47
Msa0622960	Msa1368120	0.811681	6.199207e-51	-8.615850e-47
Msa0622960	Msa1370860	0.831766	1.385712e-55	-8.615850e-47
Msa0622960	Msa1373330	0.807739	4.363397e-50	-8.615850e-47
Msa0622960	Msa1379760	0.804836	1.784421e-49	-8.615850e-47
Msa0622960	Msa1404190	0.849855	2.473485e-60	-8.615850e-47
Msa0622960	Msa1407460	0.817747	2.809414e-52	-8.615850e-47
Msa0622960	Msa1442850	0.808343	3.245515e-50	-8.615850e-47
Msa0622960	Msa1460470	0.805706	1.172626e-49	-8.615850e-47
Msa0622960	Msa1465080	0.815017	1.146782e-51	-8.615850e-47
Msa0622960	Msa1466990	0.806423	8.286155e-50	-8.615850e-47
Msa0128860	Msa0622960	0.802522	5.390195e-49	-8.615850e-47
Msa0129060	Msa0622960	0.802862	4.586525e-49	-8.615850e-47
Msa0143430	Msa0622960	0.855411	6.423364e-62	-8.615850e-47
Msa0169570	Msa0622960	0.809552	1.788786e-50	-8.615850e-47
Msa0172960	Msa0622960	-0.801105	1.053359e-48	-8.615850e-47
Msa0173820	Msa0622960	0.804935	1.701058e-49	-8.615850e-47
Msa0174730	Msa0622960	0.800358	1.496605e-48	-8.615850e-47
Msa0174770	Msa0622960	0.813566	2.398373e-51	-8.615850e-47
Msa0199880	Msa0622960	0.829182	5.939300e-55	-8.615850e-47
Msa0230550	Msa0622960	-0.816749	4.711272e-52	-8.615850e-47
Msa0369450	Msa0622960	0.833021	6.773916e-56	-8.615850e-47
Msa0376300	Msa0622960	0.805730	1.159278e-49	-8.615850e-47
Msa0390030	Msa0622960	0.820834	5.563630e-53	-8.615850e-47
Msa0414800	Msa0622960	0.808103	3.649760e-50	-8.615850e-47
Msa0425220	Msa0622960	0.801254	9.821380e-49	-8.615850e-47
Msa0425940	Msa0622960	0.809124	2.209199e-50	-8.615850e-47
Msa0427150	Msa0622960	0.817553	3.106999e-52	-8.615850e-47
Msa0441130	Msa0622960	0.831872	1.304867e-55	-8.615850e-47
Msa0465790	Msa0622960	0.801993	6.927118e-49	-8.615850e-47
Msa0471440	Msa0622960	0.806033	1.001093e-49	-8.615850e-47
Msa0471590	Msa0622960	0.811932	5.467393e-51	-8.615850e-47
Msa0241780	Msa0622960	0.812044	5.168381e-51	-8.615850e-47
Msa0242230	Msa0622960	0.805816	1.112202e-49	-8.615850e-47
Msa0264570	Msa0622960	0.805301	1.426062e-49	-8.615850e-47
Msa0267970	Msa0622960	0.812811	3.512673e-51	-8.615850e-47
Msa0280610	Msa0622960	0.810236	1.274054e-50	-8.615850e-47
Msa0300120	Msa0622960	0.811081	8.365669e-51	-8.615850e-47
Msa0310030	Msa0622960	0.801016	1.098646e-48	-8.615850e-47
Msa0314720	Msa0622960	0.818333	2.070471e-52	-8.615850e-47
Msa0322890	Msa0622960	0.833465	5.252336e-56	-8.615850e-47
Msa0488020	Msa0622960	0.829826	4.140950e-55	-8.615850e-47
Msa0508590	Msa0622960	0.804668	1.934091e-49	-8.615850e-47
Msa0508630	Msa0622960	0.819625	1.052942e-52	-8.615850e-47
Msa0552810	Msa0622960	0.852555	4.275401e-61	-8.615850e-47
Msa0581070	Msa0622960	0.808327	3.270194e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0622960	MtrunA17_Chr8g0377971	78.723	94	15	1	14	102	756	849	1.68e-42	148
Msa0622960	MtrunA17_Chr3g0121961	67.021	94	26	1	14	102	746	839	6.59e-36	129

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0622960	AT1G31230.1	59.036	83	34	22	104	764	846	1.24e-28	109
Msa0622960	AT4G19710.2	57.831	83	35	22	104	769	851	2.85e-28	108
Msa0622960	AT4G19710.1	58.824	68	28	22	89	769	836	3.19e-22	90.9

Find 54 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCAAACTCCGGGTCAAATTT+TGG	0.204913	4_2:+75757466	None:intergenic
TAGCTTGTTTATCTTGTATA+CGG	0.229519	4_2:-75756481	Msa0622960:three_prime_UTR
GGAGGGGTTGTTGCAGGATA+TGG	0.232776	4_2:+75756340	None:intergenic
CTGTCTCCATTAATCTTGTT+TGG	0.265081	4_2:-75756449	Msa0622960:three_prime_UTR
CGACTCCCAAACAAGATTAA+TGG	0.286022	4_2:+75756443	None:intergenic
GCTTAATTACAATGTCAATT+TGG	0.292506	4_2:-75757756	Msa0622960:CDS
GTTCTTACCCGTAGTGGTTC+TGG	0.302693	4_2:+75757611	None:intergenic
TTACGACTTGCCTCATATCT+AGG	0.323640	4_2:-75756616	Msa0622960:three_prime_UTR
CATCCATTTGCACAATTGTC+GGG	0.343333	4_2:-75756751	Msa0622960:CDS
TGTCTCCATTAATCTTGTTT+GGG	0.352484	4_2:-75756448	Msa0622960:three_prime_UTR
ATAATTCTCGCAAGGGAATC+TGG	0.369464	4_2:-75757677	Msa0622960:CDS
ATAGTTCGCGGGCCAGGAGC+TGG	0.412636	4_2:-75756676	Msa0622960:three_prime_UTR
GATGTTCCTCTCGAGTTGTT+TGG	0.414825	4_2:-75756546	Msa0622960:three_prime_UTR
TAATTTAGACTGTCTCGAAT+AGG	0.417800	4_2:-75756392	Msa0622960:three_prime_UTR
TCTTGTTTCTTTGCAAACTC+CGG	0.422683	4_2:+75757454	None:intergenic
CAAGAAGATGCTGACAATGC+TGG	0.432766	4_2:-75757436	Msa0622960:CDS
GTGACTTGAGCACCAGCTCC+TGG	0.443485	4_2:+75756664	None:intergenic
ATGATAAATAAATGACATGT+AGG	0.449507	4_2:-75757778	None:intergenic
AAGAAGATGCTGACAATGCT+GGG	0.463024	4_2:-75757435	Msa0622960:CDS
TCTGGCACTAGACTTTCAAT+TGG	0.463231	4_2:+75757629	None:intergenic
CTTGTTTCTTTGCAAACTCC+GGG	0.470800	4_2:+75757455	None:intergenic
TCATCCATTTGCACAATTGT+CGG	0.476880	4_2:-75756752	Msa0622960:CDS
GACCCCGACAATTGTGCAAA+TGG	0.481521	4_2:+75756748	None:intergenic
CAAACAAGATTAATGGAGAC+AGG	0.481712	4_2:+75756450	None:intergenic
CCTCTGATAGTTCGCGGGCC+AGG	0.482336	4_2:-75756682	Msa0622960:three_prime_UTR
ACAAAGCTCTGTTGTTGTAC+AGG	0.489559	4_2:-75757702	Msa0622960:CDS
AGCTTGTTTATCTTGTATAC+GGG	0.500385	4_2:-75756480	Msa0622960:three_prime_UTR
TCAGGCGTGTGCATCAGCTC+AGG	0.537437	4_2:-75757506	Msa0622960:intron
TGATGGAGCGCCTAGATATG+AGG	0.553770	4_2:+75756606	None:intergenic
AAATTGGTTCTTACCCGTAG+TGG	0.559667	4_2:+75757605	None:intergenic
GTCTAGTGCCAGAACCACTA+CGG	0.560015	4_2:-75757619	Msa0622960:CDS
GCTGGTGCTCAAGTCACAGC+GGG	0.561102	4_2:-75756658	Msa0622960:three_prime_UTR
TTGCAGAATGACATCTATGA+TGG	0.572950	4_2:+75756589	None:intergenic
TCTAGTGCCAGAACCACTAC+GGG	0.573079	4_2:-75757618	Msa0622960:intron
AATCAACCTCTGATAGTTCG+CGG	0.577271	4_2:-75756688	Msa0622960:three_prime_UTR
TACAGGTGATAATTCTCGCA+AGG	0.579848	4_2:-75757685	Msa0622960:CDS
ATCAACCTCTGATAGTTCGC+GGG	0.582260	4_2:-75756687	Msa0622960:three_prime_UTR
GGTGCTCAAGTCACAGCGGG+TGG	0.586582	4_2:-75756655	Msa0622960:three_prime_UTR
ATTGAGATACGTGGGAGTGG+TGG	0.596662	4_2:-75756822	Msa0622960:CDS
GTCAATTTGGTTCATGACTG+TGG	0.601513	4_2:-75757743	Msa0622960:CDS
TTCCTCTCGAGTTGTTTGGT+AGG	0.603997	4_2:-75756542	Msa0622960:three_prime_UTR
CCTGGCCCGCGAACTATCAG+AGG	0.604093	4_2:+75756682	None:intergenic
ATCCATTTGCACAATTGTCG+GGG	0.609087	4_2:-75756750	Msa0622960:CDS
GGTATTGAGATACGTGGGAG+TGG	0.611935	4_2:-75756825	Msa0622960:CDS
GGGTTGTTGCAGGATATGGA+TGG	0.623937	4_2:+75756344	None:intergenic
ACAGGTGATAATTCTCGCAA+GGG	0.634755	4_2:-75757684	Msa0622960:CDS
TAATTGCATTCACAACAAGA+AGG	0.639179	4_2:-75756717	Msa0622960:three_prime_UTR
AACCTACCAAACAACTCGAG+AGG	0.648392	4_2:+75756540	None:intergenic
AGAGCTGCGAAGATACAAGA+AGG	0.656222	4_2:-75756776	Msa0622960:CDS
TTGTAGGTATTGAGATACGT+GGG	0.666439	4_2:-75756830	Msa0622960:intron
TTTGTAGGTATTGAGATACG+TGG	0.669349	4_2:-75756831	Msa0622960:intron
ACAGCTACCAAAATTTGACC+CGG	0.679982	4_2:-75757473	Msa0622960:CDS
AGAAGATGCTGACAATGCTG+GGG	0.682028	4_2:-75757434	Msa0622960:CDS
AGCTGGTGCTCAAGTCACAG+CGG	0.710898	4_2:-75756659	Msa0622960:three_prime_UTR

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTATGAAATAAAGTTT+AGG	+	chr4_2:75756989-75757008	None:intergenic	10.0%
!!!	TTTTTTATGAAATAAAGTTT+AGG	+	chr4_2:75756989-75757008	None:intergenic	10.0%
!!	AGAAAATAATGTATGTAATA+TGG	+	chr4_2:75756839-75756858	None:intergenic	15.0%
!!	ATCTTGAAAAACATAATTAA+TGG	+	chr4_2:75757183-75757202	None:intergenic	15.0%
!!!	ATTAATTATGTTTTTCAAGA+TGG	-	chr4_2:75757182-75757201	Msa0622960:intron	15.0%
!!!	AATAGGCATTTTAATAATTT+TGG	-	chr4_2:75757744-75757763	Msa0622960:CDS	15.0%
!!	AGAAAATAATGTATGTAATA+TGG	+	chr4_2:75756839-75756858	None:intergenic	15.0%
!!	ATCTTGAAAAACATAATTAA+TGG	+	chr4_2:75757183-75757202	None:intergenic	15.0%
!!!	ATTAATTATGTTTTTCAAGA+TGG	-	chr4_2:75757182-75757201	Msa0622960:intron	15.0%
!!!	AATAGGCATTTTAATAATTT+TGG	-	chr4_2:75757744-75757763	Msa0622960:CDS	15.0%
!!	ACTGCATAATTTATAGAAAT+TGG	+	chr4_2:75756533-75756552	None:intergenic	20.0%
!!	CAGATCTATTATAGAAATTT+AGG	+	chr4_2:75757042-75757061	None:intergenic	20.0%
!!!	ATCGACATTACAAAATTTTA+TGG	+	chr4_2:75757556-75757575	None:intergenic	20.0%
!!	ACTGCATAATTTATAGAAAT+TGG	+	chr4_2:75756533-75756552	None:intergenic	20.0%
!!	CAGATCTATTATAGAAATTT+AGG	+	chr4_2:75757042-75757061	None:intergenic	20.0%
!!!	ATCGACATTACAAAATTTTA+TGG	+	chr4_2:75757556-75757575	None:intergenic	20.0%
!	GCTTAATTACAATGTCAATT+TGG	-	chr4_2:75756363-75756382	Msa0622960:three_prime_UTR	25.0%
!	TCTATAAATTATGCAGTTCA+TGG	-	chr4_2:75756536-75756555	Msa0622960:three_prime_UTR	25.0%
!!!	CTACTTTTATTAAGTTTGAC+AGG	+	chr4_2:75756914-75756933	None:intergenic	25.0%
!	AAATAAAGTTTAGGTGAGTT+GGG	+	chr4_2:75756980-75756999	None:intergenic	25.0%
!!	ATCTGAAGTAAAAGTGTAAA+AGG	-	chr4_2:75757057-75757076	Msa0622960:intron	25.0%
!!!	TAGCTTGTTTATCTTGTATA+CGG	-	chr4_2:75757638-75757657	Msa0622960:CDS	25.0%
!	GCTTAATTACAATGTCAATT+TGG	-	chr4_2:75756363-75756382	Msa0622960:three_prime_UTR	25.0%
!	TCTATAAATTATGCAGTTCA+TGG	-	chr4_2:75756536-75756555	Msa0622960:three_prime_UTR	25.0%
!!!	CTACTTTTATTAAGTTTGAC+AGG	+	chr4_2:75756914-75756933	None:intergenic	25.0%
!	AAATAAAGTTTAGGTGAGTT+GGG	+	chr4_2:75756980-75756999	None:intergenic	25.0%
!!	ATCTGAAGTAAAAGTGTAAA+AGG	-	chr4_2:75757057-75757076	Msa0622960:intron	25.0%
!!!	TAGCTTGTTTATCTTGTATA+CGG	-	chr4_2:75757638-75757657	Msa0622960:CDS	25.0%
!!!	CTGTTTTGATGATTTCATTC+AGG	-	chr4_2:75756595-75756614	Msa0622960:three_prime_UTR	30.0%
	GAAATAAAGTTTAGGTGAGT+TGG	+	chr4_2:75756981-75757000	None:intergenic	30.0%
	TTCCTATGTTTGTACTTTGT+AGG	-	chr4_2:75757273-75757292	Msa0622960:intron	30.0%
	TACCTACAAAGTACAAACAT+AGG	+	chr4_2:75757278-75757297	None:intergenic	30.0%
	TAATTGCATTCACAACAAGA+AGG	-	chr4_2:75757402-75757421	Msa0622960:intron	30.0%
!	AGCTTGTTTATCTTGTATAC+GGG	-	chr4_2:75757639-75757658	Msa0622960:CDS	30.0%
!	TGTCTCCATTAATCTTGTTT+GGG	-	chr4_2:75757671-75757690	Msa0622960:CDS	30.0%
	TAATTTAGACTGTCTCGAAT+AGG	-	chr4_2:75757727-75757746	Msa0622960:CDS	30.0%
!!!	CTGTTTTGATGATTTCATTC+AGG	-	chr4_2:75756595-75756614	Msa0622960:three_prime_UTR	30.0%
	GAAATAAAGTTTAGGTGAGT+TGG	+	chr4_2:75756981-75757000	None:intergenic	30.0%
	TTCCTATGTTTGTACTTTGT+AGG	-	chr4_2:75757273-75757292	Msa0622960:intron	30.0%
	TACCTACAAAGTACAAACAT+AGG	+	chr4_2:75757278-75757297	None:intergenic	30.0%
	TAATTGCATTCACAACAAGA+AGG	-	chr4_2:75757402-75757421	Msa0622960:intron	30.0%
!	AGCTTGTTTATCTTGTATAC+GGG	-	chr4_2:75757639-75757658	Msa0622960:CDS	30.0%
!	TGTCTCCATTAATCTTGTTT+GGG	-	chr4_2:75757671-75757690	Msa0622960:CDS	30.0%
	TAATTTAGACTGTCTCGAAT+AGG	-	chr4_2:75757727-75757746	Msa0622960:CDS	30.0%
!	TCTTGTTTCTTTGCAAACTC+CGG	+	chr4_2:75756668-75756687	None:intergenic	35.0%
!	AATAAAAGTAGAGTGCTCAC+AGG	-	chr4_2:75756923-75756942	Msa0622960:intron	35.0%
!	GGAAGCAAATTTAGAGTGTT+TGG	-	chr4_2:75756953-75756972	Msa0622960:intron	35.0%
!!	ACACTTTTTACTTCAGCTTG+TGG	+	chr4_2:75757087-75757106	None:intergenic	35.0%
!!	TTTGTAGGTATTGAGATACG+TGG	-	chr4_2:75757288-75757307	Msa0622960:intron	35.0%
!!	TTGTAGGTATTGAGATACGT+GGG	-	chr4_2:75757289-75757308	Msa0622960:intron	35.0%
!	TGGTGGATGTGACAAAAAAA+AGG	-	chr4_2:75757314-75757333	Msa0622960:intron	35.0%
	TCATCCATTTGCACAATTGT+CGG	-	chr4_2:75757367-75757386	Msa0622960:intron	35.0%
	TTGCAGAATGACATCTATGA+TGG	+	chr4_2:75757533-75757552	None:intergenic	35.0%
	CAAACAAGATTAATGGAGAC+AGG	+	chr4_2:75757672-75757691	None:intergenic	35.0%
	CTGTCTCCATTAATCTTGTT+TGG	-	chr4_2:75757670-75757689	Msa0622960:CDS	35.0%
!	TCTTGTTTCTTTGCAAACTC+CGG	+	chr4_2:75756668-75756687	None:intergenic	35.0%
!	AATAAAAGTAGAGTGCTCAC+AGG	-	chr4_2:75756923-75756942	Msa0622960:intron	35.0%
!	GGAAGCAAATTTAGAGTGTT+TGG	-	chr4_2:75756953-75756972	Msa0622960:intron	35.0%
!!	ACACTTTTTACTTCAGCTTG+TGG	+	chr4_2:75757087-75757106	None:intergenic	35.0%
!!	TTTGTAGGTATTGAGATACG+TGG	-	chr4_2:75757288-75757307	Msa0622960:intron	35.0%
!!	TTGTAGGTATTGAGATACGT+GGG	-	chr4_2:75757289-75757308	Msa0622960:intron	35.0%
!	TGGTGGATGTGACAAAAAAA+AGG	-	chr4_2:75757314-75757333	Msa0622960:intron	35.0%
	TCATCCATTTGCACAATTGT+CGG	-	chr4_2:75757367-75757386	Msa0622960:intron	35.0%
	TTGCAGAATGACATCTATGA+TGG	+	chr4_2:75757533-75757552	None:intergenic	35.0%
	CAAACAAGATTAATGGAGAC+AGG	+	chr4_2:75757672-75757691	None:intergenic	35.0%
	CTGTCTCCATTAATCTTGTT+TGG	-	chr4_2:75757670-75757689	Msa0622960:CDS	35.0%
!	GTCAATTTGGTTCATGACTG+TGG	-	chr4_2:75756376-75756395	Msa0622960:three_prime_UTR	40.0%
	ACAAAGCTCTGTTGTTGTAC+AGG	-	chr4_2:75756417-75756436	Msa0622960:three_prime_UTR	40.0%
	TACAGGTGATAATTCTCGCA+AGG	-	chr4_2:75756434-75756453	Msa0622960:three_prime_UTR	40.0%
	ACAGGTGATAATTCTCGCAA+GGG	-	chr4_2:75756435-75756454	Msa0622960:three_prime_UTR	40.0%
	ATAATTCTCGCAAGGGAATC+TGG	-	chr4_2:75756442-75756461	Msa0622960:three_prime_UTR	40.0%
	TCTGGCACTAGACTTTCAAT+TGG	+	chr4_2:75756493-75756512	None:intergenic	40.0%
!	AAATTGGTTCTTACCCGTAG+TGG	+	chr4_2:75756517-75756536	None:intergenic	40.0%
	AAACAGCCATGTCAAGCAAT+TGG	+	chr4_2:75756581-75756600	None:intergenic	40.0%
	ACAGCTACCAAAATTTGACC+CGG	-	chr4_2:75756646-75756665	Msa0622960:three_prime_UTR	40.0%
!	CTTGTTTCTTTGCAAACTCC+GGG	+	chr4_2:75756667-75756686	None:intergenic	40.0%
	AAGAAGATGCTGACAATGCT+GGG	-	chr4_2:75756684-75756703	Msa0622960:three_prime_UTR	40.0%
!!	CGTTTCAACCTTGCTTTTCT+TGG	+	chr4_2:75756887-75756906	None:intergenic	40.0%
!!	GTGTTACCGTTGACTTATGT+TGG	-	chr4_2:75757103-75757122	Msa0622960:intron	40.0%
!!	ACCGTTGACTTATGTTGGAT+TGG	-	chr4_2:75757108-75757127	Msa0622960:intron	40.0%
	GCCAATCCAACATAAGTCAA+CGG	+	chr4_2:75757112-75757131	None:intergenic	40.0%
!	GTTGACTTATGTTGGATTGG+CGG	-	chr4_2:75757111-75757130	Msa0622960:intron	40.0%
!!	TTGACTTATGTTGGATTGGC+GGG	-	chr4_2:75757112-75757131	Msa0622960:intron	40.0%
	CATCCATTTGCACAATTGTC+GGG	-	chr4_2:75757368-75757387	Msa0622960:intron	40.0%
	ATCCATTTGCACAATTGTCG+GGG	-	chr4_2:75757369-75757388	Msa0622960:intron	40.0%
	AATCAACCTCTGATAGTTCG+CGG	-	chr4_2:75757431-75757450	Msa0622960:intron	40.0%
	TTACGACTTGCCTCATATCT+AGG	-	chr4_2:75757503-75757522	Msa0622960:CDS	40.0%
	CGACTCCCAAACAAGATTAA+TGG	+	chr4_2:75757679-75757698	None:intergenic	40.0%
!	GTCAATTTGGTTCATGACTG+TGG	-	chr4_2:75756376-75756395	Msa0622960:three_prime_UTR	40.0%
	ACAAAGCTCTGTTGTTGTAC+AGG	-	chr4_2:75756417-75756436	Msa0622960:three_prime_UTR	40.0%
	TACAGGTGATAATTCTCGCA+AGG	-	chr4_2:75756434-75756453	Msa0622960:three_prime_UTR	40.0%
	ACAGGTGATAATTCTCGCAA+GGG	-	chr4_2:75756435-75756454	Msa0622960:three_prime_UTR	40.0%
	ATAATTCTCGCAAGGGAATC+TGG	-	chr4_2:75756442-75756461	Msa0622960:three_prime_UTR	40.0%
	TCTGGCACTAGACTTTCAAT+TGG	+	chr4_2:75756493-75756512	None:intergenic	40.0%
!	AAATTGGTTCTTACCCGTAG+TGG	+	chr4_2:75756517-75756536	None:intergenic	40.0%
	AAACAGCCATGTCAAGCAAT+TGG	+	chr4_2:75756581-75756600	None:intergenic	40.0%
	ACAGCTACCAAAATTTGACC+CGG	-	chr4_2:75756646-75756665	Msa0622960:three_prime_UTR	40.0%
!	CTTGTTTCTTTGCAAACTCC+GGG	+	chr4_2:75756667-75756686	None:intergenic	40.0%
	AAGAAGATGCTGACAATGCT+GGG	-	chr4_2:75756684-75756703	Msa0622960:three_prime_UTR	40.0%
!!	CGTTTCAACCTTGCTTTTCT+TGG	+	chr4_2:75756887-75756906	None:intergenic	40.0%
!!	GTGTTACCGTTGACTTATGT+TGG	-	chr4_2:75757103-75757122	Msa0622960:intron	40.0%
!!	ACCGTTGACTTATGTTGGAT+TGG	-	chr4_2:75757108-75757127	Msa0622960:intron	40.0%
	GCCAATCCAACATAAGTCAA+CGG	+	chr4_2:75757112-75757131	None:intergenic	40.0%
!	GTTGACTTATGTTGGATTGG+CGG	-	chr4_2:75757111-75757130	Msa0622960:intron	40.0%
!!	TTGACTTATGTTGGATTGGC+GGG	-	chr4_2:75757112-75757131	Msa0622960:intron	40.0%
	CATCCATTTGCACAATTGTC+GGG	-	chr4_2:75757368-75757387	Msa0622960:intron	40.0%
	ATCCATTTGCACAATTGTCG+GGG	-	chr4_2:75757369-75757388	Msa0622960:intron	40.0%
	AATCAACCTCTGATAGTTCG+CGG	-	chr4_2:75757431-75757450	Msa0622960:intron	40.0%
	TTACGACTTGCCTCATATCT+AGG	-	chr4_2:75757503-75757522	Msa0622960:CDS	40.0%
	CGACTCCCAAACAAGATTAA+TGG	+	chr4_2:75757679-75757698	None:intergenic	40.0%
!	CCATGTCAAGCAATTGGTTC+TGG	+	chr4_2:75756575-75756594	None:intergenic	45.0%
	CCAGAACCAATTGCTTGACA+TGG	-	chr4_2:75756572-75756591	Msa0622960:three_prime_UTR	45.0%
!	GCAAACTCCGGGTCAAATTT+TGG	+	chr4_2:75756656-75756675	None:intergenic	45.0%
	CAAGAAGATGCTGACAATGC+TGG	-	chr4_2:75756683-75756702	Msa0622960:three_prime_UTR	45.0%
	AGAAGATGCTGACAATGCTG+GGG	-	chr4_2:75756685-75756704	Msa0622960:three_prime_UTR	45.0%
!!!	CCTTGCTTTTCTTGGCTTGT+AGG	+	chr4_2:75756879-75756898	None:intergenic	45.0%
	CCTACAAGCCAAGAAAAGCA+AGG	-	chr4_2:75756876-75756895	Msa0622960:intron	45.0%
!	AAAGTAGAGTGCTCACAGGT+TGG	-	chr4_2:75756927-75756946	Msa0622960:intron	45.0%
	AGAGCTGCGAAGATACAAGA+AGG	-	chr4_2:75757343-75757362	Msa0622960:intron	45.0%
	ATCAACCTCTGATAGTTCGC+GGG	-	chr4_2:75757432-75757451	Msa0622960:CDS	45.0%
!	GATGTTCCTCTCGAGTTGTT+TGG	-	chr4_2:75757573-75757592	Msa0622960:intron	45.0%
!	TTCCTCTCGAGTTGTTTGGT+AGG	-	chr4_2:75757577-75757596	Msa0622960:intron	45.0%
	AACCTACCAAACAACTCGAG+AGG	+	chr4_2:75757582-75757601	None:intergenic	45.0%
!	CCATGTCAAGCAATTGGTTC+TGG	+	chr4_2:75756575-75756594	None:intergenic	45.0%
	CCAGAACCAATTGCTTGACA+TGG	-	chr4_2:75756572-75756591	Msa0622960:three_prime_UTR	45.0%
!	GCAAACTCCGGGTCAAATTT+TGG	+	chr4_2:75756656-75756675	None:intergenic	45.0%
	CAAGAAGATGCTGACAATGC+TGG	-	chr4_2:75756683-75756702	Msa0622960:three_prime_UTR	45.0%
	AGAAGATGCTGACAATGCTG+GGG	-	chr4_2:75756685-75756704	Msa0622960:three_prime_UTR	45.0%
!!!	CCTTGCTTTTCTTGGCTTGT+AGG	+	chr4_2:75756879-75756898	None:intergenic	45.0%
	CCTACAAGCCAAGAAAAGCA+AGG	-	chr4_2:75756876-75756895	Msa0622960:intron	45.0%
!	AAAGTAGAGTGCTCACAGGT+TGG	-	chr4_2:75756927-75756946	Msa0622960:intron	45.0%
	AGAGCTGCGAAGATACAAGA+AGG	-	chr4_2:75757343-75757362	Msa0622960:intron	45.0%
	ATCAACCTCTGATAGTTCGC+GGG	-	chr4_2:75757432-75757451	Msa0622960:CDS	45.0%
!	GATGTTCCTCTCGAGTTGTT+TGG	-	chr4_2:75757573-75757592	Msa0622960:intron	45.0%
!	TTCCTCTCGAGTTGTTTGGT+AGG	-	chr4_2:75757577-75757596	Msa0622960:intron	45.0%
	AACCTACCAAACAACTCGAG+AGG	+	chr4_2:75757582-75757601	None:intergenic	45.0%
	GTCTAGTGCCAGAACCACTA+CGG	-	chr4_2:75756500-75756519	Msa0622960:three_prime_UTR	50.0%
	TCTAGTGCCAGAACCACTAC+GGG	-	chr4_2:75756501-75756520	Msa0622960:three_prime_UTR	50.0%
	GTTCTTACCCGTAGTGGTTC+TGG	+	chr4_2:75756511-75756530	None:intergenic	50.0%
!!	TAGAGTGCTCACAGGTTGGT+AGG	-	chr4_2:75756931-75756950	Msa0622960:intron	50.0%
!!	AGAGTGCTCACAGGTTGGTA+GGG	-	chr4_2:75756932-75756951	Msa0622960:intron	50.0%
!	GGTATTGAGATACGTGGGAG+TGG	-	chr4_2:75757294-75757313	Msa0622960:intron	50.0%
	ATTGAGATACGTGGGAGTGG+TGG	-	chr4_2:75757297-75757316	Msa0622960:intron	50.0%
	GACCCCGACAATTGTGCAAA+TGG	+	chr4_2:75757374-75757393	None:intergenic	50.0%
!	TGATGGAGCGCCTAGATATG+AGG	+	chr4_2:75757516-75757535	None:intergenic	50.0%
	GTCTAGTGCCAGAACCACTA+CGG	-	chr4_2:75756500-75756519	Msa0622960:three_prime_UTR	50.0%
	TCTAGTGCCAGAACCACTAC+GGG	-	chr4_2:75756501-75756520	Msa0622960:three_prime_UTR	50.0%
	GTTCTTACCCGTAGTGGTTC+TGG	+	chr4_2:75756511-75756530	None:intergenic	50.0%
!!	TAGAGTGCTCACAGGTTGGT+AGG	-	chr4_2:75756931-75756950	Msa0622960:intron	50.0%
!!	AGAGTGCTCACAGGTTGGTA+GGG	-	chr4_2:75756932-75756951	Msa0622960:intron	50.0%
!	GGTATTGAGATACGTGGGAG+TGG	-	chr4_2:75757294-75757313	Msa0622960:intron	50.0%
	ATTGAGATACGTGGGAGTGG+TGG	-	chr4_2:75757297-75757316	Msa0622960:intron	50.0%
	GACCCCGACAATTGTGCAAA+TGG	+	chr4_2:75757374-75757393	None:intergenic	50.0%
!	TGATGGAGCGCCTAGATATG+AGG	+	chr4_2:75757516-75757535	None:intergenic	50.0%
	AGCTGGTGCTCAAGTCACAG+CGG	-	chr4_2:75757460-75757479	Msa0622960:CDS	55.0%
	AGCTGGTGCTCAAGTCACAG+CGG	-	chr4_2:75757460-75757479	Msa0622960:CDS	55.0%
	TCAGGCGTGTGCATCAGCTC+AGG	-	chr4_2:75756613-75756632	Msa0622960:three_prime_UTR	60.0%
	GTGACTTGAGCACCAGCTCC+TGG	+	chr4_2:75757458-75757477	None:intergenic	60.0%
	GCTGGTGCTCAAGTCACAGC+GGG	-	chr4_2:75757461-75757480	Msa0622960:CDS	60.0%
	TCAGGCGTGTGCATCAGCTC+AGG	-	chr4_2:75756613-75756632	Msa0622960:three_prime_UTR	60.0%
	GTGACTTGAGCACCAGCTCC+TGG	+	chr4_2:75757458-75757477	None:intergenic	60.0%
	GCTGGTGCTCAAGTCACAGC+GGG	-	chr4_2:75757461-75757480	Msa0622960:CDS	60.0%
	CCTGGCCCGCGAACTATCAG+AGG	+	chr4_2:75757440-75757459	None:intergenic	65.0%
	CCTCTGATAGTTCGCGGGCC+AGG	-	chr4_2:75757437-75757456	Msa0622960:CDS	65.0%
	ATAGTTCGCGGGCCAGGAGC+TGG	-	chr4_2:75757443-75757462	Msa0622960:CDS	65.0%
	GGTGCTCAAGTCACAGCGGG+TGG	-	chr4_2:75757464-75757483	Msa0622960:CDS	65.0%
	CCTGGCCCGCGAACTATCAG+AGG	+	chr4_2:75757440-75757459	None:intergenic	65.0%
	CCTCTGATAGTTCGCGGGCC+AGG	-	chr4_2:75757437-75757456	Msa0622960:CDS	65.0%
	ATAGTTCGCGGGCCAGGAGC+TGG	-	chr4_2:75757443-75757462	Msa0622960:CDS	65.0%
	GGTGCTCAAGTCACAGCGGG+TGG	-	chr4_2:75757464-75757483	Msa0622960:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	75756352	75757789	75756352	ID=Msa0622960;Name=Msa0622960
chr4_2	mRNA	75756352	75757789	75756352	ID=Msa0622960-mRNA-1;Parent=Msa0622960;Name=Msa0622960-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0\|1\|0.66\|1\|1\|1\|3\|390\|122
chr4_2	exon	75756352	75756846	75756352	ID=Msa0622960-mRNA-1:exon:23001;Parent=Msa0622960-mRNA-1
chr4_2	exon	75757432	75757524	75757432	ID=Msa0622960-mRNA-1:exon:23000;Parent=Msa0622960-mRNA-1
chr4_2	exon	75757619	75757789	75757619	ID=Msa0622960-mRNA-1:exon:22999;Parent=Msa0622960-mRNA-1
chr4_2	CDS	75757619	75757789	75757619	ID=Msa0622960-mRNA-1:cds;Parent=Msa0622960-mRNA-1
chr4_2	CDS	75757432	75757524	75757432	ID=Msa0622960-mRNA-1:cds;Parent=Msa0622960-mRNA-1
chr4_2	CDS	75756742	75756846	75756742	ID=Msa0622960-mRNA-1:cds;Parent=Msa0622960-mRNA-1
chr4_2	three_prime_UTR	75756352	75756741	75756352	ID=Msa0622960-mRNA-1:three_prime_utr;Parent=Msa0622960-mRNA-1

Gene Sequence	>Msa0622960 ATGACATGTAGGCTTAATTACAATGTCAATTTGGTTCATGACTGTGGTTATTCAAAATCAACATCACAAAGCTCTGTTGTTGTACAGGTGATAATTCTCGCAAGGGAATCTGGTTTAAAGCTAGAGCTGTCTAATATTCCAATTGAAAGTCTAGTGCCAGAACCACTACGGGCGTGTGCATCAGCTCAGGAGTTTATGCAACAGCTACCAAAATTTGACCCGGAGTTTGCAAAGAAACAAGAAGATGCTGACAATGCTGGGGAAGTATTGAGATACGTGGGAGTGGTGGATGTGACAAAAAAAAGGAGTTGTAGAGCTGCGAAGATACAAGAAGGATCATCCATTTGCACAATTGTCGGGGTCAGATAA
Protein sequence	>Msa0622960 MTCRLNYNVNLVHDCGYSKSTSQSSVVVQVIILARESGLKLELSNIPIESLVPEPLRACASAQEFMQQLPKFDPEFAKKQEDADNAGEVLRYVGVVDVTKKRSCRAAKIQEGSSICTIVGVR*