AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1050200


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1050200 MtrunA17_Chr7g0219021 89.189 74 8 0 4 77 1 74 3.23e-42 138
Msa1050200 MtrunA17_Chr7g0219031 75.000 76 19 0 4 79 1 76 2.32e-39 126
Msa1050200 MtrunA17_Chr4g0061451 56.250 80 31 2 4 80 1 79 5.72e-21 83.6
Msa1050200 MtrunA17_Chr3g0086861 51.852 81 35 2 4 80 1 81 8.34e-20 82.0
Msa1050200 MtrunA17_Chr3g0121631 41.558 77 41 1 4 80 1 73 7.91e-15 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1050200 AT5G61030.2 58.824 68 28 0 4 71 1 68 1.40e-22 89.0
Msa1050200 AT5G61030.1 58.824 68 28 0 4 71 1 68 1.40e-22 89.0
Msa1050200 AT1G74230.1 46.753 77 35 2 4 80 1 71 4.11e-17 73.9
Msa1050200 AT4G13850.4 38.961 77 42 2 4 80 1 72 5.07e-12 57.8
Msa1050200 AT4G13850.3 38.961 77 42 2 4 80 1 72 6.19e-12 57.8
Msa1050200 AT4G13850.2 38.961 77 42 2 4 80 1 72 7.30e-12 57.8
Msa1050200 AT4G13850.1 38.961 77 42 2 4 80 1 72 7.97e-12 57.8

Find 8 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGGCGTTCTTTGGTAAAA+TGG 0.181499 7_2:-14274408 Msa1050200:CDS
TGTGCTTGACAGCAGAATTC+TGG 0.314240 7_2:+14274375 None:intergenic
GTTTGAGCAATGGCGTTCTT+TGG 0.330058 7_2:-14274416 Msa1050200:intron
CGCTATGGAGAAGTTCTTGA+TGG 0.363305 7_2:-14274150 Msa1050200:CDS
ATGGCGTTCTTTGGTAAAAT+GGG 0.448549 7_2:-14274407 Msa1050200:CDS
TCATATAGCACTGATGATAG+TGG 0.504538 7_2:-14274192 Msa1050200:CDS
AATACTCATTATTGGATGAA+CGG 0.544630 7_2:-14276703 None:intergenic
CTGATTGCTTGAAATAACGA+AGG 0.656640 7_2:+14274326 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATGATTTTATTTAAC+AGG + chr7_2:14274394-14274413 None:intergenic 10.0%
!! AGTCTAAATAAAAAAAAAAT+TGG - chr7_2:14274991-14275010 Msa1050200:intron 10.0%
!! TTATAACTATTCAATAATTT+CGG - chr7_2:14276057-14276076 Msa1050200:intron 10.0%
!!! ATCATGTTAAATTTTAAAAA+TGG - chr7_2:14276521-14276540 Msa1050200:intron 10.0%
!!! AAAACTAATGCAATTTTTTT+CGG - chr7_2:14274441-14274460 Msa1050200:intron 15.0%
!! ACTAATAAACAAAAAATAGA+AGG - chr7_2:14274658-14274677 Msa1050200:intron 15.0%
!!! TTTTTTGAAATTTACCAAAA+CGG - chr7_2:14274949-14274968 Msa1050200:intron 15.0%
!!! CCTAATTATTTGTTTTTTTT+GGG + chr7_2:14275094-14275113 None:intergenic 15.0%
!! CCAAAAAAAACAAATAATTA+GGG - chr7_2:14275092-14275111 Msa1050200:intron 15.0%
!!! TTTTTTTTCTTAGATTTTTG+CGG - chr7_2:14275460-14275479 Msa1050200:intron 15.0%
!!! TGCTATTTTAATTCTTTTTA+TGG - chr7_2:14276239-14276258 Msa1050200:intron 15.0%
!!! GCAATTTTGTAAAATTTACA+GGG + chr7_2:14274215-14274234 None:intergenic 20.0%
!! AATTAGTGAAAAATCATTTC+GGG + chr7_2:14274262-14274281 None:intergenic 20.0%
!! GAATTAGTGAAAAATCATTT+CGG + chr7_2:14274263-14274282 None:intergenic 20.0%
!! AAGAAAAAAAATCAAGGATA+AGG + chr7_2:14274568-14274587 None:intergenic 20.0%
!! AATCAGAAGAAAAAAAATCA+AGG + chr7_2:14274574-14274593 None:intergenic 20.0%
!!! TTTTTTTTCTTCTGATTTTC+TGG - chr7_2:14274577-14274596 Msa1050200:intron 20.0%
!!! TTTTCAATGGAAAAATAGTT+CGG + chr7_2:14274758-14274777 None:intergenic 20.0%
!!! TGAGTTTTTTCTTTATTAAG+GGG + chr7_2:14274898-14274917 None:intergenic 20.0%
!!! CTGAGTTTTTTCTTTATTAA+GGG + chr7_2:14274899-14274918 None:intergenic 20.0%
!!! ACTGAGTTTTTTCTTTATTA+AGG + chr7_2:14274900-14274919 None:intergenic 20.0%
!! CCCAAAAAAAACAAATAATT+AGG - chr7_2:14275091-14275110 Msa1050200:intron 20.0%
!!! CCCTAATTATTTGTTTTTTT+TGG + chr7_2:14275095-14275114 None:intergenic 20.0%
!! TGAAATCAGAATTGAAAAAT+GGG + chr7_2:14275224-14275243 None:intergenic 20.0%
!!! TTCTTATTTACCTGTTAATT+GGG - chr7_2:14275261-14275280 Msa1050200:intron 20.0%
!!! TTTAATTCTTTTTATGGAAC+CGG - chr7_2:14276245-14276264 Msa1050200:intron 20.0%
!! TCATAAGTTACAATTAACAA+TGG - chr7_2:14276293-14276312 Msa1050200:intron 20.0%
!! GGCAATTTTGTAAAATTTAC+AGG + chr7_2:14274216-14274235 None:intergenic 25.0%
! TTAAAATCCGAATTACTCAT+GGG + chr7_2:14274237-14274256 None:intergenic 25.0%
!! TTTAAAATCCGAATTACTCA+TGG + chr7_2:14274238-14274257 None:intergenic 25.0%
! GATTATTCATAGGCATATTT+CGG + chr7_2:14274298-14274317 None:intergenic 25.0%
! AAATTCAAAACTGCTGAAAT+GGG + chr7_2:14274337-14274356 None:intergenic 25.0%
! AAAATTCAAAACTGCTGAAA+TGG + chr7_2:14274338-14274357 None:intergenic 25.0%
!!! TAGTCAAAAATTTGGTTTTC+GGG + chr7_2:14274632-14274651 None:intergenic 25.0%
!!! CTAGTCAAAAATTTGGTTTT+CGG + chr7_2:14274633-14274652 None:intergenic 25.0%
!!! CACTTAAACACTTTTTTCAA+TGG + chr7_2:14274771-14274790 None:intergenic 25.0%
!!! CATTGAAAAAAGTGTTTAAG+TGG - chr7_2:14274769-14274788 Msa1050200:intron 25.0%
!!! TATTTGTTTTTTTTGGGCTT+TGG + chr7_2:14275088-14275107 None:intergenic 25.0%
! GTGAAATCAGAATTGAAAAA+TGG + chr7_2:14275225-14275244 None:intergenic 25.0%
!! GTTCTTATTTACCTGTTAAT+TGG - chr7_2:14275260-14275279 Msa1050200:intron 25.0%
!!! TTTATCAGTATTTTTGACCT+AGG - chr7_2:14275303-14275322 Msa1050200:intron 25.0%
! TTTATCGTACAAAATTCAGT+TGG - chr7_2:14275353-14275372 Msa1050200:intron 25.0%
! TTATCGTACAAAATTCAGTT+GGG - chr7_2:14275354-14275373 Msa1050200:intron 25.0%
! TTTGAGTGATGTTAATGTTA+TGG - chr7_2:14275436-14275455 Msa1050200:intron 25.0%
! AATGAATGAATAGAGTTTGA+TGG - chr7_2:14275503-14275522 Msa1050200:intron 25.0%
!!! ACTTTAAGACGTAAAAATCA+CGG - chr7_2:14275570-14275589 Msa1050200:intron 25.0%
! TATTCAATAATTTCGGTACT+TGG - chr7_2:14276064-14276083 Msa1050200:intron 25.0%
!!! TTAAAAAAGGGTTCTAAATG+GGG + chr7_2:14276136-14276155 None:intergenic 25.0%
!!! CTTAAAAAAGGGTTCTAAAT+GGG + chr7_2:14276137-14276156 None:intergenic 25.0%
!!! TCAAATTTTAATCGTAATGC+AGG - chr7_2:14276561-14276580 Msa1050200:intron 25.0%
!! TACCAATTTTTCATCATATC+AGG - chr7_2:14276677-14276696 Msa1050200:intron 25.0%
! AACCTGATATGATGAAAAAT+TGG + chr7_2:14276682-14276701 None:intergenic 25.0%
AAAATTGCCCATGAGTAATT+CGG - chr7_2:14274227-14274246 Msa1050200:intron 30.0%
! TCTGATTGCTGATTATTCAT+AGG + chr7_2:14274308-14274327 None:intergenic 30.0%
! GAGTCTAAACGATGAAATTT+TGG - chr7_2:14274498-14274517 Msa1050200:intron 30.0%
!! CGAAAACCAAATTTTTGACT+AGG - chr7_2:14274631-14274650 Msa1050200:intron 30.0%
GTTGTTCCTAGTCAAAAATT+TGG + chr7_2:14274640-14274659 None:intergenic 30.0%
!! AAAAGTGTTTAAGTGGTGTT+CGG - chr7_2:14274776-14274795 Msa1050200:intron 30.0%
AGACAGAGAGAAAATCTAAA+CGG - chr7_2:14275014-14275033 Msa1050200:intron 30.0%
CTAATAGAAACCCAATTAAC+AGG + chr7_2:14275274-14275293 None:intergenic 30.0%
TTCGATATTAAGCATTACCT+AGG + chr7_2:14275323-14275342 None:intergenic 30.0%
! TGTAGATTTGTGTGTTTTAC+CGG - chr7_2:14275602-14275621 Msa1050200:intron 30.0%
TGTAGTTGCTTATTTGTACA+CGG + chr7_2:14275845-14275864 None:intergenic 30.0%
!!! AGTGGTAATAACAGGTTTTT+TGG + chr7_2:14275929-14275948 None:intergenic 30.0%
AATACATCAAACAGAGTTCA+TGG + chr7_2:14276020-14276039 None:intergenic 30.0%
ATAATTTCGGTACTTGGAAA+TGG - chr7_2:14276070-14276089 Msa1050200:intron 30.0%
TAATTTCGGTACTTGGAAAT+GGG - chr7_2:14276071-14276090 Msa1050200:intron 30.0%
!! GCTTAAAAAAGGGTTCTAAA+TGG + chr7_2:14276138-14276157 None:intergenic 30.0%
! TTGACCACCAAAATCTATTT+TGG + chr7_2:14276203-14276222 None:intergenic 30.0%
! TAAGTGATACTAATTTTCCG+TGG - chr7_2:14276326-14276345 Msa1050200:intron 30.0%
TGAACGGTTAAGATCAATGT+AGG - chr7_2:14274093-14274112 Msa1050200:CDS 35.0%
AATCCAATCCGAACAAATCA+AGG - chr7_2:14274806-14274825 Msa1050200:intron 35.0%
!! ATTCCTTGATTTGTTCGGAT+TGG + chr7_2:14274812-14274831 None:intergenic 35.0%
!! ATCGCATTCCTTGATTTGTT+CGG + chr7_2:14274817-14274836 None:intergenic 35.0%
! TTTTCTCACACTATCCGTTT+TGG + chr7_2:14274966-14274985 None:intergenic 35.0%
!!! TTGTTTTTTTTGGGCTTTGG+AGG + chr7_2:14275085-14275104 None:intergenic 35.0%
! CGTTTTCATAGTGTTGTCAA+CGG - chr7_2:14275530-14275549 Msa1050200:intron 35.0%
! GTAGATTTGTGTGTTTTACC+GGG - chr7_2:14275603-14275622 Msa1050200:intron 35.0%
! TAGATTTGTGTGTTTTACCG+GGG - chr7_2:14275604-14275623 Msa1050200:intron 35.0%
! GTATATTATTTTCTCTCCCC+CGG + chr7_2:14275624-14275643 None:intergenic 35.0%
! TCGCGGATTACGTAAAATAA+TGG - chr7_2:14275707-14275726 Msa1050200:intron 35.0%
ATAGCATCACTATACTGTAG+TGG + chr7_2:14275791-14275810 None:intergenic 35.0%
GCTTATTTGTACACGGAAAA+AGG + chr7_2:14275838-14275857 None:intergenic 35.0%
AACCTGTTATTACCACTATG+AGG - chr7_2:14275932-14275951 Msa1050200:intron 35.0%
TTCCTCATAGTGGTAATAAC+AGG + chr7_2:14275937-14275956 None:intergenic 35.0%
ATCATTTCTCTTCCTCATAG+TGG + chr7_2:14275947-14275966 None:intergenic 35.0%
TTGTATAATAGCCAAGAAGC+AGG - chr7_2:14276099-14276118 Msa1050200:intron 35.0%
TGTATAATAGCCAAGAAGCA+GGG - chr7_2:14276100-14276119 Msa1050200:intron 35.0%
TGTAGGGAAGGCTTAAAAAA+GGG + chr7_2:14276148-14276167 None:intergenic 35.0%
TTGTAGGGAAGGCTTAAAAA+AGG + chr7_2:14276149-14276168 None:intergenic 35.0%
! TTTTAAGCCTTCCCTACAAA+TGG - chr7_2:14276150-14276169 Msa1050200:intron 35.0%
TTCCCTACAAATGGTGATTT+GGG - chr7_2:14276159-14276178 Msa1050200:intron 35.0%
!! CCAAAATCTATTTTGGAGGA+TGG + chr7_2:14276196-14276215 None:intergenic 35.0%
! CCATCCTCCAAAATAGATTT+TGG - chr7_2:14276193-14276212 Msa1050200:intron 35.0%
! TCCTCCAAAATAGATTTTGG+TGG - chr7_2:14276196-14276215 Msa1050200:intron 35.0%
!! ACCACCAAAATCTATTTTGG+AGG + chr7_2:14276200-14276219 None:intergenic 35.0%
! AATTCTTTTTATGGAACCGG+TGG - chr7_2:14276248-14276267 Msa1050200:intron 35.0%
ACCTTGAAAACATCAAACCA+CGG + chr7_2:14276346-14276365 None:intergenic 35.0%
!! TGTTTTCAAGGTTTGAGCAA+TGG - chr7_2:14276354-14276373 Msa1050200:intron 35.0%
AATGGCGTTCTTTGGTAAAA+TGG - chr7_2:14276372-14276391 Msa1050200:intron 35.0%
ATGGCGTTCTTTGGTAAAAT+GGG - chr7_2:14276373-14276392 Msa1050200:intron 35.0%
CTGATTGCTTGAAATAACGA+AGG + chr7_2:14276457-14276476 None:intergenic 35.0%
! TCATCTGCCAAGCTTTTTAT+TGG - chr7_2:14276484-14276503 Msa1050200:intron 35.0%
CATACCTCCAATAAAAAGCT+TGG + chr7_2:14276494-14276513 None:intergenic 35.0%
!! TCATATAGCACTGATGATAG+TGG - chr7_2:14276588-14276607 Msa1050200:intron 35.0%
CGGTTAAGATCAATGTAGGA+TGG - chr7_2:14274097-14274116 Msa1050200:CDS 40.0%
TCTTAGAAGGGACTTCTTAG+GGG + chr7_2:14274541-14274560 None:intergenic 40.0%
CTCTTAGAAGGGACTTCTTA+GGG + chr7_2:14274542-14274561 None:intergenic 40.0%
TCTCTTAGAAGGGACTTCTT+AGG + chr7_2:14274543-14274562 None:intergenic 40.0%
GGATAAGGACTCTCTTAGAA+GGG + chr7_2:14274553-14274572 None:intergenic 40.0%
AGGATAAGGACTCTCTTAGA+AGG + chr7_2:14274554-14274573 None:intergenic 40.0%
!! CTGATTTTCTGGCCAAGTTT+TGG - chr7_2:14274588-14274607 Msa1050200:intron 40.0%
CGTACCTTGAAAAGCGATTA+TGG + chr7_2:14275168-14275187 None:intergenic 40.0%
TTTCATAGTGTTGTCAACGG+CGG - chr7_2:14275533-14275552 Msa1050200:intron 40.0%
! AGATTTGTGTGTTTTACCGG+GGG - chr7_2:14275605-14275624 Msa1050200:intron 40.0%
GCAACTCTGTTGTCAAATGA+CGG + chr7_2:14275816-14275835 None:intergenic 40.0%
AGAGAAATGATGAACGCTAG+AGG - chr7_2:14275956-14275975 Msa1050200:intron 40.0%
GAGAAATGATGAACGCTAGA+GGG - chr7_2:14275957-14275976 Msa1050200:intron 40.0%
AAACAGAGTTCATGGTTGTG+TGG + chr7_2:14276012-14276031 None:intergenic 40.0%
TTGAATGTATCCCTGCTTCT+TGG + chr7_2:14276113-14276132 None:intergenic 40.0%
CAAATCACCATTTGTAGGGA+AGG + chr7_2:14276160-14276179 None:intergenic 40.0%
CTTCCCTACAAATGGTGATT+TGG - chr7_2:14276158-14276177 Msa1050200:intron 40.0%
TCCCTACAAATGGTGATTTG+GGG - chr7_2:14276160-14276179 Msa1050200:intron 40.0%
TCCCCAAATCACCATTTGTA+GGG + chr7_2:14276164-14276183 None:intergenic 40.0%
ATCCCCAAATCACCATTTGT+AGG + chr7_2:14276165-14276184 None:intergenic 40.0%
! TGGTGATTTGGGGATGAAAT+CGG - chr7_2:14276170-14276189 Msa1050200:intron 40.0%
AAGTACAAACATTCCACCAC+CGG + chr7_2:14276267-14276286 None:intergenic 40.0%
! TCCGTGGTTTGATGTTTTCA+AGG - chr7_2:14276342-14276361 Msa1050200:intron 40.0%
AAAAAGCTTGGCAGATGACA+TGG + chr7_2:14276482-14276501 None:intergenic 40.0%
! TCTGCCAAGCTTTTTATTGG+AGG - chr7_2:14276487-14276506 Msa1050200:intron 40.0%
CATAGCGAGAAAAAGCTTCT+CGG + chr7_2:14276617-14276636 None:intergenic 40.0%
GATCGGATTGCTCCAAAACT+TGG + chr7_2:14274603-14274622 None:intergenic 45.0%
!! TGGTTTTCGGGCTACTTGAT+CGG + chr7_2:14274620-14274639 None:intergenic 45.0%
ACATCATATAAGCAGCCACG+TGG - chr7_2:14275056-14275075 Msa1050200:intron 45.0%
!! GCAGCCATAATCGCTTTTCA+AGG - chr7_2:14275161-14275180 Msa1050200:intron 45.0%
TGGGATTTAGCGACAGGATT+GGG + chr7_2:14275678-14275697 None:intergenic 45.0%
TTGGGATTTAGCGACAGGAT+TGG + chr7_2:14275679-14275698 None:intergenic 45.0%
AAATCCCAACCTGTCATTCG+CGG - chr7_2:14275690-14275709 Msa1050200:intron 45.0%
TAATCCGCGAATGACAGGTT+GGG + chr7_2:14275697-14275716 None:intergenic 45.0%
TTACGTAATCCGCGAATGAC+AGG + chr7_2:14275702-14275721 None:intergenic 45.0%
!! TGGTTTGTTCAAACTCCGCT+AGG - chr7_2:14275727-14275746 Msa1050200:intron 45.0%
! TCTTTTTATGGAACCGGTGG+TGG - chr7_2:14276251-14276270 Msa1050200:intron 45.0%
GTTTGAGCAATGGCGTTCTT+TGG - chr7_2:14276364-14276383 Msa1050200:intron 45.0%
TGTGCTTGACAGCAGAATTC+TGG + chr7_2:14276408-14276427 None:intergenic 45.0%
!! CGAGAAGCTTTTTCTCGCTA+TGG - chr7_2:14276615-14276634 Msa1050200:intron 45.0%
CGCTATGGAGAAGTTCTTGA+TGG - chr7_2:14276630-14276649 Msa1050200:intron 45.0%
!! TTATTTATTAATTGAATATA+TGG + chr7_2:14274152-14274171 None:intergenic 5.0%
TAGTGTTGTCAACGGCGGAT+TGG - chr7_2:14275538-14275557 Msa1050200:intron 50.0%
! AGTGTTGTCAACGGCGGATT+GGG - chr7_2:14275539-14275558 Msa1050200:intron 50.0%
! ACAGGTTGGGATTTAGCGAC+AGG + chr7_2:14275684-14275703 None:intergenic 50.0%
GTAATCCGCGAATGACAGGT+TGG + chr7_2:14275698-14275717 None:intergenic 50.0%
CAATGCACAATCTCGCCTAG+CGG + chr7_2:14275745-14275764 None:intergenic 50.0%
GGACGCGCGAGAAATGGATA+GGG + chr7_2:14274194-14274213 None:intergenic 55.0%
TTACAGGGACGCGCGAGAAA+TGG + chr7_2:14274200-14274219 None:intergenic 55.0%
GGGACGCGCGAGAAATGGAT+AGG + chr7_2:14274195-14274214 None:intergenic 60.0%
!! GGGCTTTGGAGGTTGCCACG+TGG + chr7_2:14275074-14275093 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 14274092 14276710 14274092 ID=Msa1050200;Name=Msa1050200
chr7_2 mRNA 14274092 14276710 14274092 ID=Msa1050200-mRNA-1;Parent=Msa1050200;Name=Msa1050200-mRNA-1;_AED=0.54;_eAED=0.54;_QI=0|0.5|0|0.66|0.5|0.33|3|0|90
chr7_2 exon 14276704 14276710 14276704 ID=Msa1050200-mRNA-1:exon:3515;Parent=Msa1050200-mRNA-1
chr7_2 exon 14274294 14274431 14274294 ID=Msa1050200-mRNA-1:exon:3514;Parent=Msa1050200-mRNA-1
chr7_2 exon 14274092 14274219 14274092 ID=Msa1050200-mRNA-1:exon:3513;Parent=Msa1050200-mRNA-1
chr7_2 CDS 14276704 14276710 14276704 ID=Msa1050200-mRNA-1:cds;Parent=Msa1050200-mRNA-1
chr7_2 CDS 14274294 14274431 14274294 ID=Msa1050200-mRNA-1:cds;Parent=Msa1050200-mRNA-1
chr7_2 CDS 14274092 14274219 14274092 ID=Msa1050200-mRNA-1:cds;Parent=Msa1050200-mRNA-1
Gene Sequence

>Msa1050200

ATGAACGCAATGGCGTTCTTTGGTAAAATGGGAAAGTTACTCCAGAATTCTGCTGTCAAGCACATCAATCATGATTTATCTATGTCTACTCCTTCGTTATTTCAAGCAATCAGATCCATGTCATCTGCCAAGCTTTTTATTGGAGGTGTTTCATATAGCACTGATGATAGTGGTCTCCGAGAAGCTTTTTCTCGCTATGGAGAAGTTCTTGATGGTATTTTTCTTTCTGACTCGCAATCTACCAATTTTTCATCATATCAGGTTGTAATATGA

Protein sequence

>Msa1050200

MNAMAFFGKMGKLLQNSAVKHINHDLSMSTPSLFQAIRSMSSAKLFIGGVSYSTDDSGLREAFSRYGEVLDGIFLSDSQSTNFSSYQVVI*