AlfalfaGEDB | MS.gene070244

M. sativa cultivar XinJiangDaYe / MS.gene070244

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070244.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070244.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070244.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene070244	0.820972	5.17E-53	-1.69E-46
MS.gene049341	MS.gene070244	0.825768	3.91E-54	-1.69E-46
MS.gene049821	MS.gene070244	0.821779	3.37E-53	-1.69E-46
MS.gene049887	MS.gene070244	0.813537	2.43E-51	-1.69E-46
MS.gene050020	MS.gene070244	0.801969	7.01E-49	-1.69E-46
MS.gene050277	MS.gene070244	0.806691	7.28E-50	-1.69E-46
MS.gene050386	MS.gene070244	0.800319	1.52E-48	-1.69E-46
MS.gene050689	MS.gene070244	0.830596	2.69E-55	-1.69E-46
MS.gene050807	MS.gene070244	0.833519	5.09E-56	-1.69E-46
MS.gene050815	MS.gene070244	0.820023	8.54E-53	-1.69E-46
MS.gene050838	MS.gene070244	0.862218	5.92E-64	-1.69E-46
MS.gene050901	MS.gene070244	0.804369	2.23E-49	-1.69E-46
MS.gene051003	MS.gene070244	0.802818	4.68E-49	-1.69E-46
MS.gene051233	MS.gene070244	0.851139	1.08E-60	-1.69E-46
MS.gene051243	MS.gene070244	0.811099	8.29E-51	-1.69E-46
MS.gene051245	MS.gene070244	0.805118	1.56E-49	-1.69E-46
MS.gene051328	MS.gene070244	0.859927	2.95E-63	-1.69E-46
MS.gene051343	MS.gene070244	0.807359	5.25E-50	-1.69E-46
MS.gene052133	MS.gene070244	0.842179	3.03E-58	-1.69E-46
MS.gene052335	MS.gene070244	0.822637	2.13E-53	-1.69E-46
MS.gene052665	MS.gene070244	0.819774	9.73E-53	-1.69E-46
MS.gene052947	MS.gene070244	0.826306	2.92E-54	-1.69E-46
MS.gene053058	MS.gene070244	0.835126	2.01E-56	-1.69E-46
MS.gene053563	MS.gene070244	0.832464	9.31E-56	-1.69E-46
MS.gene053564	MS.gene070244	0.825892	3.66E-54	-1.69E-46
MS.gene053581	MS.gene070244	0.83146	1.65E-55	-1.69E-46
MS.gene053590	MS.gene070244	0.809153	2.18E-50	-1.69E-46
MS.gene054823	MS.gene070244	0.812199	4.78E-51	-1.69E-46
MS.gene055311	MS.gene070244	0.815397	9.44E-52	-1.69E-46
MS.gene055469	MS.gene070244	0.801561	8.50E-49	-1.69E-46
MS.gene056181	MS.gene070244	0.82981	4.18E-55	-1.69E-46
MS.gene056215	MS.gene070244	0.82019	7.82E-53	-1.69E-46
MS.gene056390	MS.gene070244	0.801533	8.61E-49	-1.69E-46
MS.gene056580	MS.gene070244	0.855333	6.77E-62	-1.69E-46
MS.gene056631	MS.gene070244	0.801688	8.00E-49	-1.69E-46
MS.gene056760	MS.gene070244	0.811773	5.92E-51	-1.69E-46
MS.gene057057	MS.gene070244	0.84542	4.11E-59	-1.69E-46
MS.gene057094	MS.gene070244	0.806549	7.79E-50	-1.69E-46
MS.gene057140	MS.gene070244	0.80237	5.79E-49	-1.69E-46
MS.gene057234	MS.gene070244	0.820608	6.27E-53	-1.69E-46
MS.gene057705	MS.gene070244	0.826182	3.12E-54	-1.69E-46
MS.gene058054	MS.gene070244	0.80394	2.74E-49	-1.69E-46
MS.gene058055	MS.gene070244	0.817634	2.98E-52	-1.69E-46
MS.gene058238	MS.gene070244	0.816097	6.59E-52	-1.69E-46
MS.gene058638	MS.gene070244	0.80438	2.22E-49	-1.69E-46
MS.gene058639	MS.gene070244	0.81014	1.34E-50	-1.69E-46
MS.gene058646	MS.gene070244	0.82908	6.29E-55	-1.69E-46
MS.gene058654	MS.gene070244	0.80583	1.10E-49	-1.69E-46
MS.gene058852	MS.gene070244	0.816175	6.33E-52	-1.69E-46
MS.gene058906	MS.gene070244	0.836143	1.11E-56	-1.69E-46
MS.gene059171	MS.gene070244	0.826008	3.43E-54	-1.69E-46
MS.gene05978	MS.gene070244	0.812354	4.42E-51	-1.69E-46
MS.gene06001	MS.gene070244	0.824578	7.48E-54	-1.69E-46
MS.gene060296	MS.gene070244	0.801353	9.37E-49	-1.69E-46
MS.gene060429	MS.gene070244	0.802135	6.48E-49	-1.69E-46
MS.gene060463	MS.gene070244	0.854414	1.25E-61	-1.69E-46
MS.gene060465	MS.gene070244	0.860801	1.60E-63	-1.69E-46
MS.gene060803	MS.gene070244	0.816718	4.79E-52	-1.69E-46
MS.gene061006	MS.gene070244	0.806722	7.16E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene070244	MS.gene88680	PPI
MS.gene30920	MS.gene070244	PPI
MS.gene061872	MS.gene070244	PPI
MS.gene070244	MS.gene072493	PPI
MS.gene070244	MS.gene04052	PPI
MS.gene070244	MS.gene84536	PPI
MS.gene070244	MS.gene035896	PPI
MS.gene070244	MS.gene061866	PPI
MS.gene88680	MS.gene070244	PPI
MS.gene070241	MS.gene070244	PPI
MS.gene070244	MS.gene64650	PPI
MS.gene070244	MS.gene048705	PPI
MS.gene070244	MS.gene061874	PPI
MS.gene070244	MS.gene061869	PPI
MS.gene061876	MS.gene070244	PPI
MS.gene012391	MS.gene070244	PPI
MS.gene070244	MS.gene04972	PPI
MS.gene070244	MS.gene004121	PPI
MS.gene060463	MS.gene070244	PPI
MS.gene070244	MS.gene009027	PPI
MS.gene070244	MS.gene061867	PPI
MS.gene070244	MS.gene04064	PPI
MS.gene070244	MS.gene008276	PPI
MS.gene31196	MS.gene070244	PPI
MS.gene070244	MS.gene30920	PPI
MS.gene070244	MS.gene05619	PPI
MS.gene070244	MS.gene04068	PPI
MS.gene070244	MS.gene035719	PPI
MS.gene070244	MS.gene061872	PPI
MS.gene070244	MS.gene89488	PPI
MS.gene070244	MS.gene66225	PPI
MS.gene070244	MS.gene70955	PPI
MS.gene070244	MS.gene79195	PPI
MS.gene070244	MS.gene61017	PPI
MS.gene070244	MS.gene04576	PPI
MS.gene070244	MS.gene09871	PPI
MS.gene070244	MS.gene012390	PPI
MS.gene070244	MS.gene08121	PPI
MS.gene070244	MS.gene64920	PPI
MS.gene070244	MS.gene064557	PPI
MS.gene070244	MS.gene04434	PPI
MS.gene070244	MS.gene04452	PPI
MS.gene070244	MS.gene04392	PPI
MS.gene070244	MS.gene89487	PPI
MS.gene035718	MS.gene070244	PPI
MS.gene070244	MS.gene010219	PPI
MS.gene070244	MS.gene04433	PPI
MS.gene070244	MS.gene040383	PPI
MS.gene070244	MS.gene038469	PPI
MS.gene009027	MS.gene070244	PPI
MS.gene070244	MS.gene016624	PPI
MS.gene78578	MS.gene070244	PPI
MS.gene070244	MS.gene73136	PPI
MS.gene060465	MS.gene070244	PPI
MS.gene070244	MS.gene049821	PPI
MS.gene070244	MS.gene022946	PPI
MS.gene070244	MS.gene05623	PPI
MS.gene028629	MS.gene070244	PPI
MS.gene070244	MS.gene58854	PPI
MS.gene070244	MS.gene035720	PPI
MS.gene070244	MS.gene31196	PPI
MS.gene04067	MS.gene070244	PPI
MS.gene71215	MS.gene070244	PPI
MS.gene061870	MS.gene070244	PPI
MS.gene070244	MS.gene035897	PPI
MS.gene070244	MS.gene049705	PPI
MS.gene070244	MS.gene070907	PPI
MS.gene070244	MS.gene05608	PPI
MS.gene071136	MS.gene070244	PPI
MS.gene070244	MS.gene68545	PPI
MS.gene070244	MS.gene070242	PPI
MS.gene070244	MS.gene88681	PPI
MS.gene070244	MS.gene82546	PPI
MS.gene070244	MS.gene048746	PPI
MS.gene056215	MS.gene070244	PPI
MS.gene070244	MS.gene60093	PPI
MS.gene070244	MS.gene08122	PPI
MS.gene070244	MS.gene09873	PPI
MS.gene070244	MS.gene028629	PPI
MS.gene070244	MS.gene035718	PPI
MS.gene070244	MS.gene022932	PPI
MS.gene070244	MS.gene043967	PPI
MS.gene070244	MS.gene010220	PPI
MS.gene035787	MS.gene070244	PPI
MS.gene070244	MS.gene62520	PPI
MS.gene070244	MS.gene060465	PPI
MS.gene070244	MS.gene08053	PPI
MS.gene070244	MS.gene018578	PPI
MS.gene070244	MS.gene035721	PPI
MS.gene070244	MS.gene061870	PPI
MS.gene070244	MS.gene018580	PPI
MS.gene070244	MS.gene004124	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070244.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070244.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070244.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070244.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070244.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070244.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070244.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070244.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070244.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070244.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070244.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070244.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070244.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070244.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070244.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene070244.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070244.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene070244.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene070244.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene070244.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene070244.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene070244.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene070244.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene070244.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene070244.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene070244.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene070244.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 27 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGCAAAGGAGGAAAGGGTTT+AGG	0.384869	8.2:+6128011	MS.gene070244:CDS
CTTGCGAGACGACGAATTGC+AGG	0.403001	8.2:-6128095	None:intergenic
GGAGGAAAGGGTTTAGGAAA+GGG	0.429069	8.2:+6128017	MS.gene070244:CDS
GGAAGGACTCTTTACGGATT+CGG	0.436444	8.2:+6128281	MS.gene070244:CDS
AGGCAGGGAAGGACTCTTTA+CGG	0.443617	8.2:+6128275	MS.gene070244:CDS
TTGCAGGCTTTGTAATTCCC+TGG	0.457591	8.2:-6128079	None:intergenic
TGTTTACGCTCTCAAGAGGC+AGG	0.493747	8.2:+6128259	MS.gene070244:CDS
GGTTCTTCGCGATAACATCC+AGG	0.500175	8.2:+6128061	MS.gene070244:CDS
AGGAAGAGGCAAAGGAGGAA+AGG	0.518412	8.2:+6128004	MS.gene070244:CDS
GGAAGAGGCAAAGGAGGAAA+GGG	0.527098	8.2:+6128005	MS.gene070244:CDS
AGGAGGAAAGGGTTTAGGAA+AGG	0.528235	8.2:+6128016	MS.gene070244:CDS
GGAAAGGGTTTAGGAAAGGG+AGG	0.536831	8.2:+6128020	MS.gene070244:CDS
CCGTAAAACTGTTACTGCGA+TGG	0.549023	8.2:+6128232	MS.gene070244:CDS
GCGATGTCAGGAAGAGGCAA+AGG	0.557914	8.2:+6127996	None:intergenic
TTCACAGCGATGTCAGGAAG+AGG	0.573275	8.2:+6127990	None:intergenic
AGGAGCAAAACGACACCGTA+AGG	0.575994	8.2:+6128040	MS.gene070244:CDS
TGTTATCGCGAAGAACCTTA+CGG	0.576355	8.2:-6128055	None:intergenic
ACGCTCTCAAGAGGCAGGGA+AGG	0.583359	8.2:+6128264	MS.gene070244:CDS
ATGTCAGGAAGAGGCAAAGG+AGG	0.592835	8.2:+6127999	MS.gene070244:CDS
AGGACTCTTTACGGATTCGG+TGG	0.600535	8.2:+6128284	MS.gene070244:CDS
ATGTTGTTTACGCTCTCAAG+AGG	0.616421	8.2:+6128255	MS.gene070244:CDS
GTTTACGCTCTCAAGAGGCA+GGG	0.624823	8.2:+6128260	MS.gene070244:CDS
ATTCGTCGTCTCGCAAGACG+TGG	0.635108	8.2:+6128101	MS.gene070244:CDS
CGTCGTCTCGCAAGACGTGG+TGG	0.642630	8.2:+6128104	MS.gene070244:CDS
TTAATCTATGAAGAAACTCG+TGG	0.649567	8.2:+6128146	MS.gene070244:CDS
GTTCTTCGCGATAACATCCA+GGG	0.657095	8.2:+6128062	MS.gene070244:CDS
GGTGGTGTGAAGCGTATCAG+TGG	0.674719	8.2:+6128122	MS.gene070244:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTAATCTATGAAGAAACTCG+TGG	+	chr8.2:6128146-6128165	MS.gene070244:CDS	30.0%
	TGTTATCGCGAAGAACCTTA+CGG	-	chr8.2:6128058-6128077	None:intergenic	40.0%
!	ATGTTGTTTACGCTCTCAAG+AGG	+	chr8.2:6128255-6128274	MS.gene070244:CDS	40.0%
!	CCATCGCAGTAACAGTTTTA+CGG	-	chr8.2:6128235-6128254	None:intergenic	40.0%
	AGGAGGAAAGGGTTTAGGAA+AGG	+	chr8.2:6128016-6128035	MS.gene070244:CDS	45.0%
	CCGTAAAACTGTTACTGCGA+TGG	+	chr8.2:6128232-6128251	MS.gene070244:CDS	45.0%
	GGAAGGACTCTTTACGGATT+CGG	+	chr8.2:6128281-6128300	MS.gene070244:CDS	45.0%
	GGAGGAAAGGGTTTAGGAAA+GGG	+	chr8.2:6128017-6128036	MS.gene070244:CDS	45.0%
	GTTCTTCGCGATAACATCCA+GGG	+	chr8.2:6128062-6128081	MS.gene070244:CDS	45.0%
!	TTGCAGGCTTTGTAATTCCC+TGG	-	chr8.2:6128082-6128101	None:intergenic	45.0%
	AGGAAGAGGCAAAGGAGGAA+AGG	+	chr8.2:6128004-6128023	MS.gene070244:CDS	50.0%
	AGGACTCTTTACGGATTCGG+TGG	+	chr8.2:6128284-6128303	MS.gene070244:CDS	50.0%
	AGGAGCAAAACGACACCGTA+AGG	+	chr8.2:6128040-6128059	MS.gene070244:CDS	50.0%
	AGGCAGGGAAGGACTCTTTA+CGG	+	chr8.2:6128275-6128294	MS.gene070244:CDS	50.0%
	ATGTCAGGAAGAGGCAAAGG+AGG	+	chr8.2:6127999-6128018	MS.gene070244:CDS	50.0%
	GGAAAGGGTTTAGGAAAGGG+AGG	+	chr8.2:6128020-6128039	MS.gene070244:CDS	50.0%
	GGAAGAGGCAAAGGAGGAAA+GGG	+	chr8.2:6128005-6128024	MS.gene070244:CDS	50.0%
	GGCAAAGGAGGAAAGGGTTT+AGG	+	chr8.2:6128011-6128030	MS.gene070244:CDS	50.0%
	GGTTCTTCGCGATAACATCC+AGG	+	chr8.2:6128061-6128080	MS.gene070244:CDS	50.0%
	GTTTACGCTCTCAAGAGGCA+GGG	+	chr8.2:6128260-6128279	MS.gene070244:CDS	50.0%
	TGTTTACGCTCTCAAGAGGC+AGG	+	chr8.2:6128259-6128278	MS.gene070244:CDS	50.0%
	ATTCGTCGTCTCGCAAGACG+TGG	+	chr8.2:6128101-6128120	MS.gene070244:CDS	55.0%
	CTTGCGAGACGACGAATTGC+AGG	-	chr8.2:6128098-6128117	None:intergenic	55.0%
	GGTGGTGTGAAGCGTATCAG+TGG	+	chr8.2:6128122-6128141	MS.gene070244:CDS	55.0%
	ACGCTCTCAAGAGGCAGGGA+AGG	+	chr8.2:6128264-6128283	MS.gene070244:CDS	60.0%
	CGTCGTCTCGCAAGACGTGG+TGG	+	chr8.2:6128104-6128123	MS.gene070244:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	6127999	6128310	6127999	ID=MS.gene070244
chr8.2	mRNA	6127999	6128310	6127999	ID=MS.gene070244.t1;Parent=MS.gene070244
chr8.2	exon	6127999	6128310	6127999	ID=MS.gene070244.t1.exon1;Parent=MS.gene070244.t1
chr8.2	CDS	6127999	6128310	6127999	ID=cds.MS.gene070244.t1;Parent=MS.gene070244.t1

Gene Sequence	>MS.gene070244 ATGTCAGGAAGAGGCAAAGGAGGAAAGGGTTTAGGAAAGGGAGGAGCAAAACGACACCGTAAGGTTCTTCGCGATAACATCCAGGGAATTACAAAGCCTGCAATTCGTCGTCTCGCAAGACGTGGTGGTGTGAAGCGTATCAGTGGTTTAATCTATGAAGAAACTCGTGGTGTTCTCAAAATCTTTCTTGAGAATGTGATTCGTGATGCTGTCACATACACTGAACATGCTCGCCGTAAAACTGTTACTGCGATGGATGTTGTTTACGCTCTCAAGAGGCAGGGAAGGACTCTTTACGGATTCGGTGGTTAG
Protein sequence	>MS.gene070244.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG