AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49347


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49347.t1 MTR_1g070495 96.350 137 5 0 1 137 1 137 6.99e-93 265
MS.gene49347.t1 MTR_7g096150 76.642 137 31 1 1 137 1 136 8.83e-67 199
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49347.t1 AT2G30570 63.043 138 45 3 1 137 1 133 7.29e-47 148

Find 42 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGTGTGTTCGGTCTTATC+TGG 0.197833 1.1:+53098202 MS.gene49347:CDS
GTTTCATCCTCTCCTGTTCT+TGG 0.207350 1.1:+53097312 MS.gene49347:CDS
TCCACCAATGCCATGGCTGC+TGG 0.247914 1.1:-53098108 None:intergenic
GGACTCAGCAACAACCTTCT+TGG 0.302937 1.1:+53098168 MS.gene49347:CDS
CTTCCCACCTTAGCCATTGT+TGG 0.376821 1.1:-53097976 None:intergenic
TTCATTTCAAGCAATTTAAA+GGG 0.388432 1.1:-53098280 None:intergenic
TAAGACCGAACACACCAAAA+AGG 0.399989 1.1:-53098197 None:intergenic
CCACCAATGCCATGGCTGCT+GGG 0.401839 1.1:-53098107 None:intergenic
CCCAGCAGCCATGGCATTGG+TGG 0.403670 1.1:+53098107 MS.gene49347:CDS
GAAGGAACCGGGCTTCCCTT+TGG 0.425729 1.1:+53098147 MS.gene49347:CDS
TTGCTTTCACTCACAGAAGA+AGG 0.432700 1.1:-53098024 None:intergenic
AGAATGAGCACTGAAGGAAC+CGG 0.435090 1.1:+53098135 MS.gene49347:CDS
TGGTGTGTTCGGTCTTATCT+GGG 0.435857 1.1:+53098203 MS.gene49347:CDS
AAGAACAACATACCAAGAAC+AGG 0.443082 1.1:-53097324 None:intergenic
GGGAAGAGTGAGGTGTTCAA+TGG 0.445647 1.1:+53097993 MS.gene49347:CDS
CTTGAGGAGGATGAAGAGTC+TGG 0.449954 1.1:+53098252 MS.gene49347:CDS
AGCAACTCAAACATTGGAAT+GGG 0.465387 1.1:+53098042 MS.gene49347:CDS
TCAGCAACAACCTTCTTGGT+TGG 0.473672 1.1:+53098172 MS.gene49347:CDS
AGTGAAAGCAACTCAAACAT+TGG 0.493591 1.1:+53098036 MS.gene49347:CDS
AGTTGTAGTGGTTGCTGAGA+TGG 0.506917 1.1:-53097244 None:intergenic
AAGAGTGAGGTGTTCAATGG+AGG 0.507963 1.1:+53097996 MS.gene49347:CDS
AAGCCCAGCAGCCATGGCAT+TGG 0.535185 1.1:+53098104 MS.gene49347:CDS
AAGCAACTCAAACATTGGAA+TGG 0.541259 1.1:+53098041 MS.gene49347:CDS
ATTGCCAACAATGGCTAAGG+TGG 0.548615 1.1:+53097972 MS.gene49347:CDS
AGAGTGAGGTGTTCAATGGA+GGG 0.548761 1.1:+53097997 MS.gene49347:CDS
AAGGTTGTTGCTGAGTCCAA+AGG 0.556907 1.1:-53098163 None:intergenic
TCTTTCATCCACCAATGCCA+TGG 0.558482 1.1:-53098115 None:intergenic
GCTGAGTCCAAAGGGAAGCC+CGG 0.571088 1.1:-53098154 None:intergenic
AGGATTGCCAACAATGGCTA+AGG 0.571754 1.1:+53097969 MS.gene49347:intron
TATCTACGCCTCGAGTCTTG+AGG 0.577922 1.1:+53098236 MS.gene49347:CDS
AGGTTGTTGCTGAGTCCAAA+GGG 0.582023 1.1:-53098162 None:intergenic
GATGAAAGAATGAGCACTGA+AGG 0.583467 1.1:+53098129 MS.gene49347:CDS
GAATGAGCACTGAAGGAACC+GGG 0.588005 1.1:+53098136 MS.gene49347:CDS
TATGTCAAGCCCAGCAGCCA+TGG 0.588442 1.1:+53098098 MS.gene49347:CDS
TTATTCAGGATTGCCAACAA+TGG 0.611540 1.1:+53097963 MS.gene49347:intron
TTGCCAACAATGGCTAAGGT+GGG 0.634166 1.1:+53097973 MS.gene49347:CDS
TTCATCCTCCTCAAGACTCG+AGG 0.637138 1.1:-53098244 None:intergenic
CTACGCCTCGAGTCTTGAGG+AGG 0.641041 1.1:+53098239 MS.gene49347:CDS
CAACATACCAAGAACAGGAG+AGG 0.652090 1.1:-53097319 None:intergenic
GGTGATCGATGCAGTTGTAG+TGG 0.659864 1.1:-53097256 None:intergenic
TGGCTAAGGTGGGAAGAGTG+AGG 0.666585 1.1:+53097983 MS.gene49347:CDS
GGAGAGGATGAAACTGCAAG+AGG 0.693611 1.1:-53097303 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGATATATATGTTTTAAGAT+TGG + chr1.1:53097469-53097488 MS.gene49347:intron 15.0%
!!! TTATTTCATAACTTTTATTG+AGG + chr1.1:53097701-53097720 MS.gene49347:intron 15.0%
!!! TTTTTCATCATTACAAAATT+GGG - chr1.1:53097647-53097666 None:intergenic 15.0%
!! TATCATGCTATTCAATATTT+AGG - chr1.1:53097363-53097382 None:intergenic 20.0%
!!! AACATTCAATCATTTTATTC+AGG + chr1.1:53097949-53097968 MS.gene49347:intron 20.0%
!!! AATATTTAGGATGAAAATCA+AGG - chr1.1:53097350-53097369 None:intergenic 20.0%
!!! ATTTGAACCATTTTATACTA+TGG + chr1.1:53097743-53097762 MS.gene49347:intron 20.0%
!!! GTTTTTCATCATTACAAAAT+TGG - chr1.1:53097648-53097667 None:intergenic 20.0%
!!! TATTTGTCGATTTTTTTTCT+CGG + chr1.1:53097668-53097687 MS.gene49347:intron 20.0%
!!! ATTTGTCGATTTTTTTTCTC+GGG + chr1.1:53097669-53097688 MS.gene49347:intron 25.0%
!!! GAATTTTATTTGTACTTCTG+TGG + chr1.1:53097442-53097461 MS.gene49347:intron 25.0%
!!! GTTTTTGATTTTGTCAAACT+CGG + chr1.1:53097574-53097593 MS.gene49347:intron 25.0%
!!! TTTACTCCATTTTTAATCGA+CGG + chr1.1:53097898-53097917 MS.gene49347:intron 25.0%
AATCGAGCCATAGTATAAAA+TGG - chr1.1:53097753-53097772 None:intergenic 30.0%
CAAAATCAAAAACCACATGT+TGG - chr1.1:53097567-53097586 None:intergenic 30.0%
TCATCATTACAAAATTGGGA+GGG - chr1.1:53097643-53097662 None:intergenic 30.0%
TTAGTGCATGTTTGATGTTA+CGG + chr1.1:53097521-53097540 MS.gene49347:intron 30.0%
TTCATCATTACAAAATTGGG+AGG - chr1.1:53097644-53097663 None:intergenic 30.0%
! ATACTATGGCTCGATTATTT+TGG + chr1.1:53097757-53097776 MS.gene49347:intron 30.0%
!!! TTATTTTGGCAATTCGATCA+CGG + chr1.1:53097771-53097790 MS.gene49347:intron 30.0%
!!! TTTGTCGATTTTTTTTCTCG+GGG + chr1.1:53097670-53097689 MS.gene49347:intron 30.0%
AAGAACAACATACCAAGAAC+AGG - chr1.1:53097327-53097346 None:intergenic 35.0%
AAGCAACTCAAACATTGGAA+TGG + chr1.1:53098041-53098060 MS.gene49347:CDS 35.0%
AGCAACTCAAACATTGGAAT+GGG + chr1.1:53098042-53098061 MS.gene49347:CDS 35.0%
AGTGAAAGCAACTCAAACAT+TGG + chr1.1:53098036-53098055 MS.gene49347:CDS 35.0%
ATTGTTGAAGATCCAACATG+TGG + chr1.1:53097552-53097571 MS.gene49347:intron 35.0%
CATCTTCAATTGCTTGAATC+AGG - chr1.1:53097803-53097822 None:intergenic 35.0%
CTGATTCAAGCAATTGAAGA+TGG + chr1.1:53097801-53097820 MS.gene49347:intron 35.0%
TTATTCAGGATTGCCAACAA+TGG + chr1.1:53097963-53097982 MS.gene49347:intron 35.0%
! AAACCTCCGTCGATTAAAAA+TGG - chr1.1:53097907-53097926 None:intergenic 35.0%
! ATTTTTAATCGACGGAGGTT+TGG + chr1.1:53097906-53097925 MS.gene49347:intron 35.0%
CAAAAAGGATCCAACCAAGA+AGG - chr1.1:53098185-53098204 None:intergenic 40.0%
CGCTCGATTGATATTTGGTT+CGG + chr1.1:53097846-53097865 MS.gene49347:intron 40.0%
TAAGACCGAACACACCAAAA+AGG - chr1.1:53098200-53098219 None:intergenic 40.0%
TGTCTCGCTCGATTGATATT+TGG + chr1.1:53097841-53097860 MS.gene49347:intron 40.0%
TTGCTTTCACTCACAGAAGA+AGG - chr1.1:53098027-53098046 None:intergenic 40.0%
! CTTCTTGGTTGGATCCTTTT+TGG + chr1.1:53098183-53098202 MS.gene49347:CDS 40.0%
! TCTTTTGTGCAAGACATGCA+CGG - chr1.1:53097281-53097300 None:intergenic 40.0%
!! ACTCCATTTTTAATCGACGG+AGG + chr1.1:53097901-53097920 MS.gene49347:intron 40.0%
!! GATGAAAGAATGAGCACTGA+AGG + chr1.1:53098129-53098148 MS.gene49347:CDS 40.0%
!! TGGATCCTTTTTGGTGTGTT+CGG + chr1.1:53098192-53098211 MS.gene49347:CDS 40.0%
AAGGTTGTTGCTGAGTCCAA+AGG - chr1.1:53098166-53098185 None:intergenic 45.0%
AGGATTGCCAACAATGGCTA+AGG + chr1.1:53097969-53097988 MS.gene49347:intron 45.0%
AGGTTGTTGCTGAGTCCAAA+GGG - chr1.1:53098165-53098184 None:intergenic 45.0%
AGTTGTAGTGGTTGCTGAGA+TGG - chr1.1:53097247-53097266 None:intergenic 45.0%
ATTGCCAACAATGGCTAAGG+TGG + chr1.1:53097972-53097991 MS.gene49347:CDS 45.0%
CAAAATTGGGAGGGTAGTCT+TGG - chr1.1:53097634-53097653 None:intergenic 45.0%
CAACATACCAAGAACAGGAG+AGG - chr1.1:53097322-53097341 None:intergenic 45.0%
GTTTCATCCTCTCCTGTTCT+TGG + chr1.1:53097312-53097331 MS.gene49347:CDS 45.0%
TCAGCAACAACCTTCTTGGT+TGG + chr1.1:53098172-53098191 MS.gene49347:CDS 45.0%
TCTTTCATCCACCAATGCCA+TGG - chr1.1:53098118-53098137 None:intergenic 45.0%
TTGCCAACAATGGCTAAGGT+GGG + chr1.1:53097973-53097992 MS.gene49347:CDS 45.0%
! AAGAGTGAGGTGTTCAATGG+AGG + chr1.1:53097996-53098015 MS.gene49347:CDS 45.0%
! AGAGTGAGGTGTTCAATGGA+GGG + chr1.1:53097997-53098016 MS.gene49347:CDS 45.0%
! CTTTTGTGCAAGACATGCAC+GGG - chr1.1:53097280-53097299 None:intergenic 45.0%
! TGGTGTGTTCGGTCTTATCT+GGG + chr1.1:53098203-53098222 MS.gene49347:CDS 45.0%
! TTGGTGTGTTCGGTCTTATC+TGG + chr1.1:53098202-53098221 MS.gene49347:CDS 45.0%
!! AGAATGAGCACTGAAGGAAC+CGG + chr1.1:53098135-53098154 MS.gene49347:CDS 45.0%
CGACGGAGGTTTGGATGATT+TGG + chr1.1:53097915-53097934 MS.gene49347:intron 50.0%
CTTCCCACCTTAGCCATTGT+TGG - chr1.1:53097979-53097998 None:intergenic 50.0%
CTTGAGGAGGATGAAGAGTC+TGG + chr1.1:53098252-53098271 MS.gene49347:CDS 50.0%
GGACTCAGCAACAACCTTCT+TGG + chr1.1:53098168-53098187 MS.gene49347:CDS 50.0%
GGAGAGGATGAAACTGCAAG+AGG - chr1.1:53097306-53097325 None:intergenic 50.0%
GGTGATCGATGCAGTTGTAG+TGG - chr1.1:53097259-53097278 None:intergenic 50.0%
TATCTACGCCTCGAGTCTTG+AGG + chr1.1:53098236-53098255 MS.gene49347:CDS 50.0%
TTCATCCTCCTCAAGACTCG+AGG - chr1.1:53098247-53098266 None:intergenic 50.0%
! GGGAAGAGTGAGGTGTTCAA+TGG + chr1.1:53097993-53098012 MS.gene49347:CDS 50.0%
!! GAATGAGCACTGAAGGAACC+GGG + chr1.1:53098136-53098155 MS.gene49347:CDS 50.0%
TATGTCAAGCCCAGCAGCCA+TGG + chr1.1:53098098-53098117 MS.gene49347:CDS 55.0%
TGGCTAAGGTGGGAAGAGTG+AGG + chr1.1:53097983-53098002 MS.gene49347:CDS 55.0%
AAGCCCAGCAGCCATGGCAT+TGG + chr1.1:53098104-53098123 MS.gene49347:CDS 60.0%
CCACCAATGCCATGGCTGCT+GGG - chr1.1:53098110-53098129 None:intergenic 60.0%
CTACGCCTCGAGTCTTGAGG+AGG + chr1.1:53098239-53098258 MS.gene49347:CDS 60.0%
GAAGGAACCGGGCTTCCCTT+TGG + chr1.1:53098147-53098166 MS.gene49347:CDS 60.0%
GCTGAGTCCAAAGGGAAGCC+CGG - chr1.1:53098157-53098176 None:intergenic 60.0%
TCCACCAATGCCATGGCTGC+TGG - chr1.1:53098111-53098130 None:intergenic 60.0%
! CCCAGCAGCCATGGCATTGG+TGG + chr1.1:53098107-53098126 MS.gene49347:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 53097237 53098287 53097237 ID=MS.gene49347
chr1.1 mRNA 53097237 53098287 53097237 ID=MS.gene49347.t1;Parent=MS.gene49347
chr1.1 exon 53097237 53097333 53097237 ID=MS.gene49347.t1.exon1;Parent=MS.gene49347.t1
chr1.1 CDS 53097237 53097333 53097237 ID=cds.MS.gene49347.t1;Parent=MS.gene49347.t1
chr1.1 exon 53097971 53098287 53097971 ID=MS.gene49347.t1.exon2;Parent=MS.gene49347.t1
chr1.1 CDS 53097971 53098287 53097971 ID=cds.MS.gene49347.t1;Parent=MS.gene49347.t1
Gene Sequence

>MS.gene49347

ATGGCAACCATCTCAGCAACCACTACAACTGCATCGATCACCCGTGCATGTCTTGCACAAAAGAGACCTCTTGCAGTTTCATCCTCTCCTGTTCTTGGTATGTTGTTCTTCCTTGATTTTCATCCTAAATATTGAATAGCATGATACATTGATACTATATCTTTCAATGTCTGATTTATTTACTTACAAGTTACAATTTTCTAGAGAATTTTATTTGTACTTCTGTGGACAGAGATATATATGTTTTAAGATTGGCACATATCTATCAATTTAGAATGCATTAGTTAGTGCATGTTTGATGTTACGGTAAGTTTGATTGTTGAAGATCCAACATGTGGTTTTTGATTTTGTCAAACTCGGTGCAAATTCAAACTTGCACTAAGTTACTAAACTTCCAAGACTACCCTCCCAATTTTGTAATGATGAAAAACTATTTGTCGATTTTTTTTCTCGGGGACTAAACTTTATTTCATAACTTTTATTGAGGACTAGAATAGTGTTTTAAAATTTGAACCATTTTATACTATGGCTCGATTATTTTGGCAATTCGATCACGGATCTGTCCTGATTCAAGCAATTGAAGATGGTTGAATCATGATTCAAATGTCTCGCTCGATTGATATTTGGTTCGGTTTTTAAAACATGAGACTAATTATGATTGTTTACTCCATTTTTAATCGACGGAGGTTTGGATGATTTGGATTGATAAATTAACATTCAATCATTTTATTCAGGATTGCCAACAATGGCTAAGGTGGGAAGAGTGAGGTGTTCAATGGAGGGAAAGCCTTCTTCTGTGAGTGAAAGCAACTCAAACATTGGAATGGGATCATCTTTGTTTGCAGCTGCATGTGCTGCAGCTATGTCAAGCCCAGCAGCCATGGCATTGGTGGATGAAAGAATGAGCACTGAAGGAACCGGGCTTCCCTTTGGACTCAGCAACAACCTTCTTGGTTGGATCCTTTTTGGTGTGTTCGGTCTTATCTGGGCTCTCTACTTTATCTACGCCTCGAGTCTTGAGGAGGATGAAGAGTCTGGATTATCCCTTTAA

Protein sequence

>MS.gene49347.t1

MATISATTTTASITRACLAQKRPLAVSSSPVLGLPTMAKVGRVRCSMEGKPSSVSESNSNIGMGSSLFAAACAAAMSSPAAMALVDERMSTEGTGLPFGLSNNLLGWILFGVFGLIWALYFIYASSLEEDEESGLSL