AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023649.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023649.01.T01 MTR_8g098335 91.406 128 11 0 1 128 445 572 1.52e-79 244
MsG0480023649.01.T01 MTR_7g077740 31.538 130 83 3 5 128 783 912 2.33e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 20 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAGCTGTTGTAATGTTAT+TGG 0.222383 4:+88087690 None:intergenic
ACAACACATGCTTGGAAGTT+TGG 0.253601 4:+88087777 None:intergenic
ATTACAACAGCTTGTTGAAA+GGG 0.423151 4:-88087682 MsG0480023649.01.T01:CDS
GTATATCATCGAATGTTTCA+TGG 0.442276 4:-88087847 MsG0480023649.01.T01:CDS
TCCATTCACTTCATCAAAAG+TGG 0.447779 4:+88087908 None:intergenic
ATGTGTTGTGAGGAAAGTTT+TGG 0.462231 4:-88087763 MsG0480023649.01.T01:CDS
CTTTCCTCACAACACATGCT+TGG 0.474299 4:+88087769 None:intergenic
CATTACAACAGCTTGTTGAA+AGG 0.512663 4:-88087683 MsG0480023649.01.T01:CDS
TGTTGTAATGTTATTGGTGA+TGG 0.522358 4:+88087696 None:intergenic
CAATTTCTTCTGCATCAACA+TGG 0.524519 4:+88087628 None:intergenic
ACTTGGCCAGACGTGAATCA+TGG 0.524559 4:-88087659 MsG0480023649.01.T01:CDS
AGTCATCCATGATTCACGTC+TGG 0.529300 4:+88087653 None:intergenic
AATGTTATTGGTGATGGATG+AGG 0.570884 4:+88087702 None:intergenic
ACAGCTTGTTGAAAGGGACT+TGG 0.590760 4:-88087676 MsG0480023649.01.T01:CDS
GATGATATACTCATGCAAGA+TGG 0.600077 4:+88087860 None:intergenic
ATGTTATTGGTGATGGATGA+GGG 0.600743 4:+88087703 None:intergenic
AGAAGAAATTGCAATGGAGG+TGG 0.605756 4:-88087616 MsG0480023649.01.T01:CDS
ACTTCCAAGCATGTGTTGTG+AGG 0.626643 4:-88087773 MsG0480023649.01.T01:CDS
TGCAGAAGAAATTGCAATGG+AGG 0.645966 4:-88087619 MsG0480023649.01.T01:CDS
TGATGCAGAAGAAATTGCAA+TGG 0.674423 4:-88087622 MsG0480023649.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GGATTTCTTGATGAAAAATA+GGG + Chr4:88087683-88087702 None:intergenic 25.0%
! TGGATTTCTTGATGAAAAAT+AGG + Chr4:88087684-88087703 None:intergenic 25.0%
ACAAGCTGTTGTAATGTTAT+TGG + Chr4:88087791-88087810 None:intergenic 30.0%
GTATATCATCGAATGTTTCA+TGG - Chr4:88087631-88087650 MsG0480023649.01.T01:CDS 30.0%
TGTTGTAATGTTATTGGTGA+TGG + Chr4:88087785-88087804 None:intergenic 30.0%
!! ATTACAACAGCTTGTTGAAA+GGG - Chr4:88087796-88087815 MsG0480023649.01.T01:CDS 30.0%
!!! GAAAAATGTTTTGGAAACAC+TGG - Chr4:88087886-88087905 MsG0480023649.01.T01:CDS 30.0%
AGATGGTACTGTCAAAAAGT+TGG + Chr4:88087604-88087623 None:intergenic 35.0%
AGGGTGAAAAATCACTATTC+TGG + Chr4:88087664-88087683 None:intergenic 35.0%
CAATTTCTTCTGCATCAACA+TGG + Chr4:88087853-88087872 None:intergenic 35.0%
GATGATATACTCATGCAAGA+TGG + Chr4:88087621-88087640 None:intergenic 35.0%
TCCATTCACTTCATCAAAAG+TGG + Chr4:88087573-88087592 None:intergenic 35.0%
TGATGCAGAAGAAATTGCAA+TGG - Chr4:88087856-88087875 MsG0480023649.01.T01:CDS 35.0%
! ATGTGTTGTGAGGAAAGTTT+TGG - Chr4:88087715-88087734 MsG0480023649.01.T01:CDS 35.0%
!! AATGTTATTGGTGATGGATG+AGG + Chr4:88087779-88087798 None:intergenic 35.0%
!! ATGTTATTGGTGATGGATGA+GGG + Chr4:88087778-88087797 None:intergenic 35.0%
!! CATTACAACAGCTTGTTGAA+AGG - Chr4:88087795-88087814 MsG0480023649.01.T01:CDS 35.0%
!! TCCACTTTTGATGAAGTGAA+TGG - Chr4:88087569-88087588 MsG0480023649.01.T01:CDS 35.0%
!!! TGTTTTGGAAACACTGGTTT+TGG - Chr4:88087892-88087911 MsG0480023649.01.T01:CDS 35.0%
ACAACACATGCTTGGAAGTT+TGG + Chr4:88087704-88087723 None:intergenic 40.0%
AGAAGAAATTGCAATGGAGG+TGG - Chr4:88087862-88087881 MsG0480023649.01.T01:CDS 40.0%
TGCAGAAGAAATTGCAATGG+AGG - Chr4:88087859-88087878 MsG0480023649.01.T01:CDS 40.0%
! GGAGGTGGAGAAAAATGTTT+TGG - Chr4:88087877-88087896 MsG0480023649.01.T01:CDS 40.0%
ACTTCCAAGCATGTGTTGTG+AGG - Chr4:88087705-88087724 MsG0480023649.01.T01:CDS 45.0%
AGTCATCCATGATTCACGTC+TGG + Chr4:88087828-88087847 None:intergenic 45.0%
CTTTCCTCACAACACATGCT+TGG + Chr4:88087712-88087731 None:intergenic 45.0%
!! ACAGCTTGTTGAAAGGGACT+TGG - Chr4:88087802-88087821 MsG0480023649.01.T01:CDS 45.0%
ACTTGGCCAGACGTGAATCA+TGG - Chr4:88087819-88087838 MsG0480023649.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr4 gene 88087557 88087943 88087557 ID=MsG0480023649.01;Name=MsG0480023649.01
Chr4 mRNA 88087557 88087943 88087557 ID=MsG0480023649.01.T01;Parent=MsG0480023649.01;Name=MsG0480023649.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|128
Chr4 exon 88087557 88087943 88087557 ID=MsG0480023649.01.T01:exon:6450;Parent=MsG0480023649.01.T01
Chr4 CDS 88087557 88087943 88087557 ID=MsG0480023649.01.T01:cds;Parent=MsG0480023649.01.T01
Gene Sequence

>MsG0480023649.01.T01

ATGGATTCTTCATCCACTTTTGATGAAGTGAATGGATTGACTGACCAACTTTTTGACAGTACCATCTTGCATGAGTATATCATCGAATGTTTCATGGAGTTATACCAGAATAGTGATTTTTCACCCTATTTTTCATCAAGAAATCCAAACTTCCAAGCATGTGTTGTGAGGAAAGTTTTGGTTAGACAGATTAATGAACTTGTTAAACTTCACTTTTTCCCTCATCCATCACCAATAACATTACAACAGCTTGTTGAAAGGGACTTGGCCAGACGTGAATCATGGATGACTATCCATGTTGATGCAGAAGAAATTGCAATGGAGGTGGAGAAAAATGTTTTGGAAACACTGGTTTTGGAAATCGTGTCTGAGATGACTATATCATAG

Protein sequence

>MsG0480023649.01.T01

MDSSSTFDEVNGLTDQLFDSTILHEYIIECFMELYQNSDFSPYFSSRNPNFQACVVRKVLVRQINELVKLHFFPHPSPITLQQLVERDLARRESWMTIHVDAEEIAMEVEKNVLETLVLEIVSEMTIS*