AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0809980


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0809980 MtrunA17_Chr4g0072411 100.000 103 0 0 1 103 59 161 5.39e-69 203
Msa0809980 MtrunA17_Chr5g0425951 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa0809980 MtrunA17_Chr4g0030431 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa0809980 MtrunA17_Chr3g0100121 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa0809980 MtrunA17_Chr3g0113681 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa0809980 MtrunA17_Chr2g0327641 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa0809980 MtrunA17_Chr3g0103351 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa0809980 MtrunA17_Chr8g0352791 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa0809980 MtrunA17_Chr3g0103821 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa0809980 MtrunA17_Chr3g0104031 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa0809980 MtrunA17_Chr7g0225731 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa0809980 MtrunA17_Chr3g0103801 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa0809980 MtrunA17_Chr3g0103881 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa0809980 MtrunA17_Chr7g0262791 100.000 103 0 0 1 103 1 103 2.26e-68 199
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0809980 AT3G53730.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa0809980 AT1G07820.2 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa0809980 AT1G07820.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa0809980 AT5G59690.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa0809980 AT3G46320.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa0809980 AT2G28740.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa0809980 AT1G07660.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa0809980 AT3G45930.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa0809980 AT5G59970.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa0809980 AT5G59970.2 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa0809980 AT1G07660.2 83.495 103 0 1 1 103 1 86 4.52e-51 155

Find 26 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAGAACCCTTTATGGTTT+TGG 0.212027 5_3:-37851363 Msa0809980:CDS
AGGCAAGGAAGAACCCTTTA+TGG 0.295802 5_3:-37851369 Msa0809980:CDS
AGGAAAAGGAGGAAAGGGTT+TGG 0.356920 5_3:-37851634 Msa0809980:CDS
GGAAAAGGAGGAAAGGGTTT+GGG 0.372310 5_3:-37851633 Msa0809980:CDS
GTTCTTCGAGATAACATTCA+AGG 0.421661 5_3:-37851582 Msa0809980:CDS
GGAAAGGGTTTGGGAAAGGG+TGG 0.425706 5_3:-37851624 Msa0809980:CDS
GGAGGAAAGGGTTTGGGAAA+GGG 0.445686 5_3:-37851627 Msa0809980:CDS
GGAAGAGGAAAAGGAGGAAA+GGG 0.454700 5_3:-37851639 Msa0809980:CDS
TCGCAAAACGGTGACTGCTA+TGG 0.458269 5_3:-37851412 Msa0809980:CDS
GAGACGACGAATCGCAGGTT+TGG 0.461583 5_3:+37851554 None:intergenic
ATCTTGCAATCAACAATGTC+AGG 0.473641 5_3:-37851660 None:intergenic
AGGAGGAAAGGGTTTGGGAA+AGG 0.475381 5_3:-37851628 Msa0809980:CDS
AGGAAGAGGAAAAGGAGGAA+AGG 0.488546 5_3:-37851640 Msa0809980:CDS
ACAATGTCAGGAAGAGGAAA+AGG 0.493795 5_3:-37851648 Msa0809980:CDS
CTGCGATTCGTCGTCTCGCA+AGG 0.531517 5_3:-37851548 Msa0809980:CDS
CAATCAACAATGTCAGGAAG+AGG 0.542220 5_3:-37851654 None:intergenic
CTTGCGAGACGACGAATCGC+AGG 0.555283 5_3:+37851549 None:intergenic
GGAACACGCTCGTCGCAAAA+CGG 0.562995 5_3:-37851424 Msa0809980:CDS
GTTTATGCACTCAAGAGGCA+AGG 0.602465 5_3:-37851384 Msa0809980:CDS
ATTCGTCGTCTCGCAAGGCG+TGG 0.608390 5_3:-37851543 Msa0809980:CDS
ATGTTGTTTATGCACTCAAG+AGG 0.622074 5_3:-37851389 Msa0809980:CDS
ATGTCAGGAAGAGGAAAAGG+AGG 0.630961 5_3:-37851645 Msa0809980:CDS
CGTCGTCTCGCAAGGCGTGG+TGG 0.632134 5_3:-37851540 Msa0809980:CDS
TCGTGATGCTGTAACCTACA+CGG 0.656428 5_3:-37851445 Msa0809980:CDS
GGTGGTGTGAAGCGTATCAG+TGG 0.661880 5_3:-37851522 Msa0809980:CDS
CTCATCTATGAAGAAACTCG+TGG 0.682677 5_3:-37851498 Msa0809980:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GTTCTTCGAGATAACATTCA+AGG - chr5_3:37851419-37851438 Msa0809980:CDS 35.0%
! ATGTTGTTTATGCACTCAAG+AGG - chr5_3:37851612-37851631 Msa0809980:CDS 35.0%
! GAAGAACCCTTTATGGTTTT+GGG - chr5_3:37851639-37851658 Msa0809980:CDS 35.0%
!! AAGAACCCTTTATGGTTTTG+GGG - chr5_3:37851640-37851659 Msa0809980:CDS 35.0%
CTCATCTATGAAGAAACTCG+TGG - chr5_3:37851503-37851522 Msa0809980:CDS 40.0%
! GGAAGAACCCTTTATGGTTT+TGG - chr5_3:37851638-37851657 Msa0809980:CDS 40.0%
!!! AGAACCCTTTATGGTTTTGG+GGG - chr5_3:37851641-37851660 Msa0809980:CDS 40.0%
CTAACCCCCAAAACCATAAA+GGG + chr5_3:37851648-37851667 None:intergenic 40.0%
ATGTCAGGAAGAGGAAAAGG+AGG - chr5_3:37851356-37851375 Msa0809980:CDS 45.0%
AGGAAGAGGAAAAGGAGGAA+AGG - chr5_3:37851361-37851380 Msa0809980:CDS 45.0%
GGAAGAGGAAAAGGAGGAAA+GGG - chr5_3:37851362-37851381 Msa0809980:CDS 45.0%
AGGAAAAGGAGGAAAGGGTT+TGG - chr5_3:37851367-37851386 Msa0809980:CDS 45.0%
GGAAAAGGAGGAAAGGGTTT+GGG - chr5_3:37851368-37851387 Msa0809980:CDS 45.0%
TCGTGATGCTGTAACCTACA+CGG - chr5_3:37851556-37851575 Msa0809980:CDS 45.0%
GTTTATGCACTCAAGAGGCA+AGG - chr5_3:37851617-37851636 Msa0809980:CDS 45.0%
AGGCAAGGAAGAACCCTTTA+TGG - chr5_3:37851632-37851651 Msa0809980:CDS 45.0%
AGGAGGAAAGGGTTTGGGAA+AGG - chr5_3:37851373-37851392 Msa0809980:CDS 50.0%
GGAGGAAAGGGTTTGGGAAA+GGG - chr5_3:37851374-37851393 Msa0809980:CDS 50.0%
TCGCAAAACGGTGACTGCTA+TGG - chr5_3:37851589-37851608 Msa0809980:CDS 50.0%
GGAAAGGGTTTGGGAAAGGG+TGG - chr5_3:37851377-37851396 Msa0809980:CDS 55.0%
GAGACGACGAATCGCAGGTT+TGG + chr5_3:37851450-37851469 None:intergenic 55.0%
GGTGGTGTGAAGCGTATCAG+TGG - chr5_3:37851479-37851498 Msa0809980:CDS 55.0%
GGAACACGCTCGTCGCAAAA+CGG - chr5_3:37851577-37851596 Msa0809980:CDS 55.0%
CTTGCGAGACGACGAATCGC+AGG + chr5_3:37851455-37851474 None:intergenic 60.0%
CTGCGATTCGTCGTCTCGCA+AGG - chr5_3:37851453-37851472 Msa0809980:CDS 60.0%
ATTCGTCGTCTCGCAAGGCG+TGG - chr5_3:37851458-37851477 Msa0809980:CDS 60.0%
GCGACGAGCGTGTTCCGTGT+AGG + chr5_3:37851573-37851592 None:intergenic 65.0%
CGTCGTCTCGCAAGGCGTGG+TGG - chr5_3:37851461-37851480 Msa0809980:CDS 70.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 37851356 37851667 37851356 ID=Msa0809980;Name=Msa0809980
chr5_3 mRNA 37851356 37851667 37851356 ID=Msa0809980-mRNA-1;Parent=Msa0809980;Name=Msa0809980-mRNA-1;_AED=0.35;_eAED=0.35;_QI=0|-1|0|1|-1|1|1|0|103
chr5_3 exon 37851356 37851667 37851356 ID=Msa0809980-mRNA-1:exon:11600;Parent=Msa0809980-mRNA-1
chr5_3 CDS 37851356 37851667 37851356 ID=Msa0809980-mRNA-1:cds;Parent=Msa0809980-mRNA-1
Gene Sequence

>Msa0809980

ATGTCAGGAAGAGGAAAAGGAGGAAAGGGTTTGGGAAAGGGTGGTGCAAAGCGTCATCGCAAAGTTCTTCGAGATAACATTCAAGGAATCACCAAACCTGCGATTCGTCGTCTCGCAAGGCGTGGTGGTGTGAAGCGTATCAGTGGTCTCATCTATGAAGAAACTCGTGGTGTTCTCAAGATATTTCTCGAGAATGTGATTCGTGATGCTGTAACCTACACGGAACACGCTCGTCGCAAAACGGTGACTGCTATGGATGTTGTTTATGCACTCAAGAGGCAAGGAAGAACCCTTTATGGTTTTGGGGGTTAG

Protein sequence

>Msa0809980

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG*