AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0901110


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0901110 MtrunA17_Chr6g0487221 93.023 172 11 1 1 172 39 209 2.73e-102 292
Msa0901110 MtrunA17_Chr8g0389751 50.617 162 57 6 1 147 1 154 4.90e-29 104
Msa0901110 MtrunA17_Chr3g0109691 52.174 92 31 2 65 156 76 154 1.49e-21 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0901110 AT5G56550.1 58.427 89 33 2 62 146 72 160 1.88e-24 94.0
Msa0901110 AT4G26288.1 51.887 106 30 5 47 144 40 132 5.51e-16 71.6

Find 26 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTAGAGATAGTTTGTTTA+AGG 0.157965 6_1:-97382780 Msa0901110:three_prime_UTR
GATGGAATTGCATACTTTAT+AGG 0.293344 6_1:+97383686 None:intergenic
CCCTTAAGTTCATCTTTATT+AGG 0.294928 6_1:-97382873 Msa0901110:CDS
CCTAATAAAGATGAACTTAA+GGG 0.332817 6_1:+97382873 None:intergenic
AAACATGAAGTGGGTGGTTA+AGG 0.347693 6_1:-97383873 Msa0901110:CDS
AGTGATTCAACTACATCTAT+TGG 0.349622 6_1:-97383842 Msa0901110:CDS
GCCTAATAAAGATGAACTTA+AGG 0.352869 6_1:+97382872 None:intergenic
AACACAATCTAGGATATAAT+TGG 0.364081 6_1:-97384054 None:intergenic
AATGAATGTGAAGGGAATAA+TGG 0.371118 6_1:-97383912 Msa0901110:CDS
ATGAAGTCATGTAGAAGTTA+TGG 0.374322 6_1:-97382966 Msa0901110:CDS
TAGAAGTTATGGAGGAGGTT+TGG 0.410155 6_1:-97382955 Msa0901110:CDS
GTTTGGATAATCATAGAATA+TGG 0.415366 6_1:-97382938 Msa0901110:CDS
TCTTCATCATCACATTCAAA+TGG 0.424478 6_1:-97383746 Msa0901110:CDS
TTCTTGTGTACAACAATTGA+AGG 0.446183 6_1:+97382819 None:intergenic
TCATCTTTATTAGGCAAAAG+AGG 0.482739 6_1:-97382864 Msa0901110:CDS
ATCTTTCACTTCTTTAGCTG+AGG 0.515540 6_1:-97383039 Msa0901110:CDS
CATCTTTATTAGGCAAAAGA+GGG 0.516192 6_1:-97382863 Msa0901110:CDS
ATTTCTTGAAATGAATGTGA+AGG 0.520984 6_1:-97383921 Msa0901110:exon
TCATGTAGAAGTTATGGAGG+AGG 0.533647 6_1:-97382960 Msa0901110:CDS
TTTCTTGAAATGAATGTGAA+GGG 0.535143 6_1:-97383920 Msa0901110:exon
AAGTCATGTAGAAGTTATGG+AGG 0.581965 6_1:-97382963 Msa0901110:CDS
TAGAATATGGTATAGTCCAA+AGG 0.592131 6_1:-97382925 Msa0901110:CDS
TTGTTCATCAGATTTGACTG+AGG 0.665115 6_1:-97383783 Msa0901110:CDS
TGCAAGAAACATGAAGTGGG+TGG 0.681358 6_1:-97383879 Msa0901110:CDS
ATAGTGCAAGAAACATGAAG+TGG 0.703522 6_1:-97383883 Msa0901110:CDS
TAGTGCAAGAAACATGAAGT+GGG 0.728595 6_1:-97383882 Msa0901110:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTTTAAAATTATTAT+CGG - chr6_1:97383578-97383597 Msa0901110:intron 0.0%
!!! TTATTTTTTAAAATTATTAT+CGG - chr6_1:97383578-97383597 Msa0901110:intron 0.0%
!! AATAAAACTTATAAAAATGT+AGG + chr6_1:97383115-97383134 None:intergenic 10.0%
!! AATAAAACTTATAAAAATGT+AGG + chr6_1:97383115-97383134 None:intergenic 10.0%
!! TAAACTAATAAATACGTAAA+AGG - chr6_1:97383396-97383415 Msa0901110:intron 15.0%
!! TAAACTAATAAATACGTAAA+AGG - chr6_1:97383396-97383415 Msa0901110:intron 15.0%
!! AAACTTATAAAAATGTAGGA+TGG + chr6_1:97383111-97383130 None:intergenic 20.0%
!! CATAATTGAATTTGATTCTT+CGG + chr6_1:97383268-97383287 None:intergenic 20.0%
!!! AATTAGAGATAGTTTGTTTA+AGG - chr6_1:97383996-97384015 Msa0901110:five_prime_UTR 20.0%
!! AAACTTATAAAAATGTAGGA+TGG + chr6_1:97383111-97383130 None:intergenic 20.0%
!! CATAATTGAATTTGATTCTT+CGG + chr6_1:97383268-97383287 None:intergenic 20.0%
!!! AATTAGAGATAGTTTGTTTA+AGG - chr6_1:97383996-97384015 Msa0901110:five_prime_UTR 20.0%
! ATTTCTTGAAATGAATGTGA+AGG - chr6_1:97382855-97382874 Msa0901110:CDS 25.0%
! TTTCTTGAAATGAATGTGAA+GGG - chr6_1:97382856-97382875 Msa0901110:CDS 25.0%
! AAGGAATCATAGTTATTACA+AGG - chr6_1:97383415-97383434 Msa0901110:intron 25.0%
!! CTGTTCTCTTTTGATAAAAA+TGG + chr6_1:97383491-97383510 None:intergenic 25.0%
! TTTAAGAATATGACACGATA+CGG - chr6_1:97383530-97383549 Msa0901110:intron 25.0%
!!! TGTTTTTAGATGTAGTGAAT+TGG - chr6_1:97383640-97383659 Msa0901110:intron 25.0%
!!! AATTAGTTTATGACTTTTGC+AGG - chr6_1:97383679-97383698 Msa0901110:intron 25.0%
!! TTGTAAGATATGCTTTTCTT+GGG + chr6_1:97383783-97383802 None:intergenic 25.0%
! GTTTGGATAATCATAGAATA+TGG - chr6_1:97383838-97383857 Msa0901110:CDS 25.0%
!!! TTTTTTGATATTGTAGCCTT+TGG + chr6_1:97383870-97383889 None:intergenic 25.0%
! ATATCAAAAAAAGCTTCAAG+AGG - chr6_1:97383879-97383898 Msa0901110:CDS 25.0%
! TATCAAAAAAAGCTTCAAGA+GGG - chr6_1:97383880-97383899 Msa0901110:CDS 25.0%
! CCTAATAAAGATGAACTTAA+GGG + chr6_1:97383906-97383925 None:intergenic 25.0%
! ATTTCTTGAAATGAATGTGA+AGG - chr6_1:97382855-97382874 Msa0901110:CDS 25.0%
! TTTCTTGAAATGAATGTGAA+GGG - chr6_1:97382856-97382875 Msa0901110:CDS 25.0%
! AAGGAATCATAGTTATTACA+AGG - chr6_1:97383415-97383434 Msa0901110:intron 25.0%
!! CTGTTCTCTTTTGATAAAAA+TGG + chr6_1:97383491-97383510 None:intergenic 25.0%
! TTTAAGAATATGACACGATA+CGG - chr6_1:97383530-97383549 Msa0901110:intron 25.0%
!!! TGTTTTTAGATGTAGTGAAT+TGG - chr6_1:97383640-97383659 Msa0901110:intron 25.0%
!!! AATTAGTTTATGACTTTTGC+AGG - chr6_1:97383679-97383698 Msa0901110:intron 25.0%
!! TTGTAAGATATGCTTTTCTT+GGG + chr6_1:97383783-97383802 None:intergenic 25.0%
! GTTTGGATAATCATAGAATA+TGG - chr6_1:97383838-97383857 Msa0901110:CDS 25.0%
!!! TTTTTTGATATTGTAGCCTT+TGG + chr6_1:97383870-97383889 None:intergenic 25.0%
! ATATCAAAAAAAGCTTCAAG+AGG - chr6_1:97383879-97383898 Msa0901110:CDS 25.0%
! TATCAAAAAAAGCTTCAAGA+GGG - chr6_1:97383880-97383899 Msa0901110:CDS 25.0%
! CCTAATAAAGATGAACTTAA+GGG + chr6_1:97383906-97383925 None:intergenic 25.0%
! TACAACATAACGTGTTTTCA+AGG - chr6_1:97382800-97382819 Msa0901110:exon 30.0%
AATGAATGTGAAGGGAATAA+TGG - chr6_1:97382864-97382883 Msa0901110:CDS 30.0%
AGTGATTCAACTACATCTAT+TGG - chr6_1:97382934-97382953 Msa0901110:CDS 30.0%
TCTTCATCATCACATTCAAA+TGG - chr6_1:97383030-97383049 Msa0901110:CDS 30.0%
GATGGAATTGCATACTTTAT+AGG + chr6_1:97383093-97383112 None:intergenic 30.0%
!!! GTCTAGTTTAGACTAGTTTT+TGG - chr6_1:97383187-97383206 Msa0901110:intron 30.0%
! GGTTGTCAATGTTTTATCAA+GGG - chr6_1:97383706-97383725 Msa0901110:CDS 30.0%
! GTTGTAAGATATGCTTTTCT+TGG + chr6_1:97383784-97383803 None:intergenic 30.0%
ATGAAGTCATGTAGAAGTTA+TGG - chr6_1:97383810-97383829 Msa0901110:CDS 30.0%
TAGAATATGGTATAGTCCAA+AGG - chr6_1:97383851-97383870 Msa0901110:CDS 30.0%
CCCTTAAGTTCATCTTTATT+AGG - chr6_1:97383903-97383922 Msa0901110:CDS 30.0%
GCCTAATAAAGATGAACTTA+AGG + chr6_1:97383907-97383926 None:intergenic 30.0%
TCATCTTTATTAGGCAAAAG+AGG - chr6_1:97383912-97383931 Msa0901110:CDS 30.0%
CATCTTTATTAGGCAAAAGA+GGG - chr6_1:97383913-97383932 Msa0901110:CDS 30.0%
TTCTTGTGTACAACAATTGA+AGG + chr6_1:97383960-97383979 None:intergenic 30.0%
! TACAACATAACGTGTTTTCA+AGG - chr6_1:97382800-97382819 Msa0901110:exon 30.0%
AATGAATGTGAAGGGAATAA+TGG - chr6_1:97382864-97382883 Msa0901110:CDS 30.0%
AGTGATTCAACTACATCTAT+TGG - chr6_1:97382934-97382953 Msa0901110:CDS 30.0%
TCTTCATCATCACATTCAAA+TGG - chr6_1:97383030-97383049 Msa0901110:CDS 30.0%
GATGGAATTGCATACTTTAT+AGG + chr6_1:97383093-97383112 None:intergenic 30.0%
!!! GTCTAGTTTAGACTAGTTTT+TGG - chr6_1:97383187-97383206 Msa0901110:intron 30.0%
! GGTTGTCAATGTTTTATCAA+GGG - chr6_1:97383706-97383725 Msa0901110:CDS 30.0%
! GTTGTAAGATATGCTTTTCT+TGG + chr6_1:97383784-97383803 None:intergenic 30.0%
ATGAAGTCATGTAGAAGTTA+TGG - chr6_1:97383810-97383829 Msa0901110:CDS 30.0%
TAGAATATGGTATAGTCCAA+AGG - chr6_1:97383851-97383870 Msa0901110:CDS 30.0%
CCCTTAAGTTCATCTTTATT+AGG - chr6_1:97383903-97383922 Msa0901110:CDS 30.0%
GCCTAATAAAGATGAACTTA+AGG + chr6_1:97383907-97383926 None:intergenic 30.0%
TCATCTTTATTAGGCAAAAG+AGG - chr6_1:97383912-97383931 Msa0901110:CDS 30.0%
CATCTTTATTAGGCAAAAGA+GGG - chr6_1:97383913-97383932 Msa0901110:CDS 30.0%
TTCTTGTGTACAACAATTGA+AGG + chr6_1:97383960-97383979 None:intergenic 30.0%
ATAGTGCAAGAAACATGAAG+TGG - chr6_1:97382893-97382912 Msa0901110:CDS 35.0%
TAGTGCAAGAAACATGAAGT+GGG - chr6_1:97382894-97382913 Msa0901110:CDS 35.0%
TTGTTCATCAGATTTGACTG+AGG - chr6_1:97382993-97383012 Msa0901110:CDS 35.0%
TAGTCTAAACTAGACATACG+TGG + chr6_1:97383182-97383201 None:intergenic 35.0%
! TTTATGACTTTTGCAGGAGA+GGG - chr6_1:97383685-97383704 Msa0901110:intron 35.0%
! GGGTTGTCAATGTTTTATCA+AGG - chr6_1:97383705-97383724 Msa0901110:CDS 35.0%
! GTCAATGTTTTATCAAGGGA+AGG - chr6_1:97383710-97383729 Msa0901110:CDS 35.0%
ATCTTTCACTTCTTTAGCTG+AGG - chr6_1:97383737-97383756 Msa0901110:CDS 35.0%
AAGTCATGTAGAAGTTATGG+AGG - chr6_1:97383813-97383832 Msa0901110:CDS 35.0%
ATAGTGCAAGAAACATGAAG+TGG - chr6_1:97382893-97382912 Msa0901110:CDS 35.0%
TAGTGCAAGAAACATGAAGT+GGG - chr6_1:97382894-97382913 Msa0901110:CDS 35.0%
TTGTTCATCAGATTTGACTG+AGG - chr6_1:97382993-97383012 Msa0901110:CDS 35.0%
TAGTCTAAACTAGACATACG+TGG + chr6_1:97383182-97383201 None:intergenic 35.0%
! TTTATGACTTTTGCAGGAGA+GGG - chr6_1:97383685-97383704 Msa0901110:intron 35.0%
! GGGTTGTCAATGTTTTATCA+AGG - chr6_1:97383705-97383724 Msa0901110:CDS 35.0%
! GTCAATGTTTTATCAAGGGA+AGG - chr6_1:97383710-97383729 Msa0901110:CDS 35.0%
ATCTTTCACTTCTTTAGCTG+AGG - chr6_1:97383737-97383756 Msa0901110:CDS 35.0%
AAGTCATGTAGAAGTTATGG+AGG - chr6_1:97383813-97383832 Msa0901110:CDS 35.0%
AAACATGAAGTGGGTGGTTA+AGG - chr6_1:97382903-97382922 Msa0901110:CDS 40.0%
! GTTTATGACTTTTGCAGGAG+AGG - chr6_1:97383684-97383703 Msa0901110:intron 40.0%
TCATGTAGAAGTTATGGAGG+AGG - chr6_1:97383816-97383835 Msa0901110:CDS 40.0%
TAGAAGTTATGGAGGAGGTT+TGG - chr6_1:97383821-97383840 Msa0901110:CDS 40.0%
AAACATGAAGTGGGTGGTTA+AGG - chr6_1:97382903-97382922 Msa0901110:CDS 40.0%
! GTTTATGACTTTTGCAGGAG+AGG - chr6_1:97383684-97383703 Msa0901110:intron 40.0%
TCATGTAGAAGTTATGGAGG+AGG - chr6_1:97383816-97383835 Msa0901110:CDS 40.0%
TAGAAGTTATGGAGGAGGTT+TGG - chr6_1:97383821-97383840 Msa0901110:CDS 40.0%
TGCAAGAAACATGAAGTGGG+TGG - chr6_1:97382897-97382916 Msa0901110:CDS 45.0%
TGCAAGAAACATGAAGTGGG+TGG - chr6_1:97382897-97382916 Msa0901110:CDS 45.0%
!! TATATATATATAAGAATTAT+TGG + chr6_1:97382758-97382777 None:intergenic 5.0%
!! TATATATATATAAGAATTAT+TGG + chr6_1:97382758-97382777 None:intergenic 5.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 97382730 97384068 97382730 ID=Msa0901110;Name=Msa0901110
chr6_1 mRNA 97382730 97384068 97382730 ID=Msa0901110-mRNA-1;Parent=Msa0901110;Name=Msa0901110-mRNA-1;_AED=0.01;_eAED=0.01;_QI=135|1|1|1|1|1|2|82|172
chr6_1 exon 97382730 97383097 97382730 ID=Msa0901110-mRNA-1:exon:17095;Parent=Msa0901110-mRNA-1
chr6_1 exon 97383701 97384068 97383701 ID=Msa0901110-mRNA-1:exon:17094;Parent=Msa0901110-mRNA-1
chr6_1 five_prime_UTR 97383934 97384068 97383934 ID=Msa0901110-mRNA-1:five_prime_utr;Parent=Msa0901110-mRNA-1
chr6_1 CDS 97383701 97383933 97383701 ID=Msa0901110-mRNA-1:cds;Parent=Msa0901110-mRNA-1
chr6_1 CDS 97382812 97383097 97382812 ID=Msa0901110-mRNA-1:cds;Parent=Msa0901110-mRNA-1
chr6_1 three_prime_UTR 97382730 97382811 97382730 ID=Msa0901110-mRNA-1:three_prime_utr;Parent=Msa0901110-mRNA-1
Gene Sequence

>Msa0901110

ATGAATGTGAAGGGAATAATGGATGATGATAGTGCAAGAAACATGAAGTGGGTGGTTAAGGAAAGTAATAGTGATTCAACTACATCTATTGGAACATTCTCAGAAGATTCAAATAATTCTATGTGTTCTTGTTCATCAGATTTGACTGAGGATGCTGATTCTTCTTCTTCATCATCACATTCAAATGGATCATTATGTGATTTCTCAGAGCTCATGAACAATCTTCCTATAAAGAGAGGGTTGTCAATGTTTTATCAAGGGAAGGCTCAATCTTTCACTTCTTTAGCTGAGGTACAAAACATAGAAGATCTTCCCAAGAAAAGCATATCTTACAACAAAAGAATGAAGTCATGTAGAAGTTATGGAGGAGGTTTGGATAATCATAGAATATGGTATAGTCCAAAGGCTACAATATCAAAAAAAGCTTCAAGAGGGCCCTTAAGTTCATCTTTATTAGGCAAAAGAGGGAGTTTATTAGAACGTTCGAGACCTTCAATTGTTGTACACAAGAACTTCTGA

Protein sequence

>Msa0901110

MNVKGIMDDDSARNMKWVVKESNSDSTTSIGTFSEDSNNSMCSCSSDLTEDADSSSSSSHSNGSLCDFSELMNNLPIKRGLSMFYQGKAQSFTSLAEVQNIEDLPKKSISYNKRMKSCRSYGGGLDNHRIWYSPKATISKKASRGPLSSSLLGKRGSLLERSRPSIVVHKNF*