AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0880780


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0880780 MtrunA17_Chr4g0068141 95.455 176 8 0 1 176 201 376 5.57e-126 358
Msa0880780 MtrunA17_Chr4g0068121 77.714 175 39 0 1 175 201 375 7.76e-103 300
Msa0880780 MtrunA17_Chr4g0036961 78.409 176 38 0 1 176 201 376 2.46e-102 299
Msa0880780 MtrunA17_Chr3g0082001 40.805 174 87 3 7 172 225 390 3.39e-42 145
Msa0880780 MtrunA17_Chr3g0134551 40.805 174 87 3 7 172 225 390 3.69e-42 145
Msa0880780 MtrunA17_Chr1g0152791 39.766 171 102 1 2 172 38 207 9.88e-42 139
Msa0880780 MtrunA17_Chr3g0134561 40.116 172 101 2 2 172 220 390 7.38e-41 141
Msa0880780 MtrunA17_Chr3g0134571 39.759 166 99 1 7 172 214 378 9.10e-40 138
Msa0880780 MtrunA17_Chr1g0152811 39.181 171 103 1 2 172 256 425 2.22e-39 138
Msa0880780 MtrunA17_Chr1g0152831 41.212 165 96 1 2 166 254 417 7.31e-39 138
Msa0880780 MtrunA17_Chr3g0104611 35.429 175 107 3 3 172 204 377 5.89e-30 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0880780 AT4G24220.1 35.673 171 109 1 2 172 219 388 3.16e-36 129
Msa0880780 AT4G24220.2 35.673 171 109 1 2 172 218 387 3.96e-36 129
Msa0880780 AT5G58750.2 32.571 175 114 3 3 173 204 378 1.58e-23 95.9
Msa0880780 AT5G58750.1 32.571 175 114 3 3 173 212 386 1.69e-23 95.9

Find 34 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTTAATAGTGTGAAATTC+TGG 0.190417 6_1:+27627283 Msa0880780:CDS
CGTTTCGTTATCCAGGAAAT+AGG 0.260788 6_1:+27626869 Msa0880780:CDS
TGCGTGATAAGGGTGAGATT+TGG 0.302571 6_1:+27627109 Msa0880780:CDS
GCAATTAAACGCTTGGTTCT+TGG 0.326986 6_1:-27626974 None:intergenic
GCGTGATAAGGGTGAGATTT+GGG 0.351508 6_1:+27627110 Msa0880780:CDS
TGTAGTTCAATGTTCGTAGA+TGG 0.355494 6_1:-27627337 None:intergenic
TGCTGTTAAGTTTGTGGAGT+TGG 0.382859 6_1:+27627053 Msa0880780:CDS
TGGACTTCATAAGACCAAAT+TGG 0.385145 6_1:+27627152 Msa0880780:CDS
GATCAGATTGTGGAGGAGTA+TGG 0.407176 6_1:+27627132 Msa0880780:CDS
CAAGCGTTTAATTGCACAAA+TGG 0.411281 6_1:+27626982 Msa0880780:CDS
TGAAAAGGAAGAGTTTGATT+TGG 0.422569 6_1:+27627077 Msa0880780:CDS
TCCAAAGTGTACCTATTTCC+TGG 0.427390 6_1:-27626880 None:intergenic
GGATTACCGTTTCGTTATCC+AGG 0.443194 6_1:+27626862 Msa0880780:CDS
GTTTGTGGAGTTGGATGAAA+AGG 0.451890 6_1:+27627062 Msa0880780:CDS
GGTGCAGTTTATGCGTGATA+AGG 0.456957 6_1:+27627098 Msa0880780:CDS
GTGCAGTTTATGCGTGATAA+GGG 0.465205 6_1:+27627099 Msa0880780:CDS
TAATCCTACCTGGCGACATA+TGG 0.474335 6_1:-27626845 None:intergenic
CATTTGTGCAATTAAACGCT+TGG 0.482702 6_1:-27626981 None:intergenic
TCCAGGAAATAGGTACACTT+TGG 0.499854 6_1:+27626879 Msa0880780:CDS
GTTTAATAGTGTGAAATTCT+GGG 0.501763 6_1:+27627284 Msa0880780:CDS
TGAGATTTGGGATCAGATTG+TGG 0.512590 6_1:+27627122 Msa0880780:CDS
TGCTCGCAAAGCAGCATGTG+TGG 0.516452 6_1:+27626932 Msa0880780:CDS
CTATTTCCTGGATAACGAAA+CGG 0.534217 6_1:-27626868 None:intergenic
ATGCGCTGCCATATGTCGCC+AGG 0.550376 6_1:+27626837 Msa0880780:CDS
GGAGCATTTCTGCGATATGA+CGG 0.555683 6_1:+27626900 Msa0880780:CDS
GTAGTTCAATGTTCGTAGAT+GGG 0.556971 6_1:-27627336 None:intergenic
GTGGGCTGCAGTTACAAAGA+AGG 0.580004 6_1:+27626951 Msa0880780:CDS
CACATGGAAGAGTATGTGGA+AGG 0.602569 6_1:+27627014 Msa0880780:CDS
GCTCGCAAAGCAGCATGTGT+GGG 0.604913 6_1:+27626933 Msa0880780:CDS
AACGAAACGGTAATCCTACC+TGG 0.609340 6_1:-27626855 None:intergenic
AATTCTGGGTGATGAAGCTG+AGG 0.665041 6_1:+27627298 Msa0880780:CDS
GCTGCCATATGTCGCCAGGT+AGG 0.675590 6_1:+27626841 Msa0880780:CDS
GATTTGGGATCAGATTGTGG+AGG 0.682099 6_1:+27627125 Msa0880780:CDS
ATTCTGGGTGATGAAGCTGA+GGG 0.787809 6_1:+27627299 Msa0880780:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GTTTAATAGTGTGAAATTCT+GGG + chr6_1:27627284-27627303 Msa0880780:CDS 25.0%
TGAAAAGGAAGAGTTTGATT+TGG + chr6_1:27627077-27627096 Msa0880780:CDS 30.0%
! ACAAGCAATTTTCTCCAATT+TGG - chr6_1:27627169-27627188 None:intergenic 30.0%
TGAAACTCAAATCGAAAAAC+TGG - chr6_1:27627207-27627226 None:intergenic 30.0%
CGTTTAATAGTGTGAAATTC+TGG + chr6_1:27627283-27627302 Msa0880780:CDS 30.0%
CTATTTCCTGGATAACGAAA+CGG - chr6_1:27626871-27626890 None:intergenic 35.0%
CATTTGTGCAATTAAACGCT+TGG - chr6_1:27626984-27627003 None:intergenic 35.0%
CAAGCGTTTAATTGCACAAA+TGG + chr6_1:27626982-27627001 Msa0880780:CDS 35.0%
!! TTTTCACATGGAAGAGTATG+TGG + chr6_1:27627010-27627029 Msa0880780:CDS 35.0%
!!! GACTTTTGCTGTTAAGTTTG+TGG + chr6_1:27627047-27627066 Msa0880780:CDS 35.0%
TGGACTTCATAAGACCAAAT+TGG + chr6_1:27627152-27627171 Msa0880780:CDS 35.0%
!!! AATTGCTTGTTTTGAAGCCT+TGG + chr6_1:27627179-27627198 Msa0880780:CDS 35.0%
CGTTTCGTTATCCAGGAAAT+AGG + chr6_1:27626869-27626888 Msa0880780:CDS 40.0%
TCCAGGAAATAGGTACACTT+TGG + chr6_1:27626879-27626898 Msa0880780:CDS 40.0%
TCCAAAGTGTACCTATTTCC+TGG - chr6_1:27626883-27626902 None:intergenic 40.0%
! GCAATTAAACGCTTGGTTCT+TGG - chr6_1:27626977-27626996 None:intergenic 40.0%
!! CAAATGGTGACGTTTTCACA+TGG + chr6_1:27626998-27627017 Msa0880780:CDS 40.0%
TGCTGTTAAGTTTGTGGAGT+TGG + chr6_1:27627053-27627072 Msa0880780:CDS 40.0%
GTTTGTGGAGTTGGATGAAA+AGG + chr6_1:27627062-27627081 Msa0880780:CDS 40.0%
GTGCAGTTTATGCGTGATAA+GGG + chr6_1:27627099-27627118 Msa0880780:CDS 40.0%
! TGAGATTTGGGATCAGATTG+TGG + chr6_1:27627122-27627141 Msa0880780:CDS 40.0%
AAATCGAAAAACTGGCACCA+AGG - chr6_1:27627199-27627218 None:intergenic 40.0%
TAATCCTACCTGGCGACATA+TGG - chr6_1:27626848-27626867 None:intergenic 45.0%
AACGAAACGGTAATCCTACC+TGG - chr6_1:27626858-27626877 None:intergenic 45.0%
GGATTACCGTTTCGTTATCC+AGG + chr6_1:27626862-27626881 Msa0880780:CDS 45.0%
! GGAGCATTTCTGCGATATGA+CGG + chr6_1:27626900-27626919 Msa0880780:CDS 45.0%
! CACATGGAAGAGTATGTGGA+AGG + chr6_1:27627014-27627033 Msa0880780:CDS 45.0%
GGTGCAGTTTATGCGTGATA+AGG + chr6_1:27627098-27627117 Msa0880780:CDS 45.0%
TGCGTGATAAGGGTGAGATT+TGG + chr6_1:27627109-27627128 Msa0880780:CDS 45.0%
GCGTGATAAGGGTGAGATTT+GGG + chr6_1:27627110-27627129 Msa0880780:CDS 45.0%
! GATTTGGGATCAGATTGTGG+AGG + chr6_1:27627125-27627144 Msa0880780:CDS 45.0%
GATCAGATTGTGGAGGAGTA+TGG + chr6_1:27627132-27627151 Msa0880780:CDS 45.0%
AATTCTGGGTGATGAAGCTG+AGG + chr6_1:27627298-27627317 Msa0880780:CDS 45.0%
ATTCTGGGTGATGAAGCTGA+GGG + chr6_1:27627299-27627318 Msa0880780:CDS 45.0%
! GTGGGCTGCAGTTACAAAGA+AGG + chr6_1:27626951-27626970 Msa0880780:CDS 50.0%
TGCTCGCAAAGCAGCATGTG+TGG + chr6_1:27626932-27626951 Msa0880780:CDS 55.0%
GCTCGCAAAGCAGCATGTGT+GGG + chr6_1:27626933-27626952 Msa0880780:CDS 55.0%
ATGCGCTGCCATATGTCGCC+AGG + chr6_1:27626837-27626856 Msa0880780:CDS 60.0%
GCTGCCATATGTCGCCAGGT+AGG + chr6_1:27626841-27626860 Msa0880780:CDS 60.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 27626823 27627353 27626823 ID=Msa0880780;Name=Msa0880780
chr6_1 mRNA 27626823 27627353 27626823 ID=Msa0880780-mRNA-1;Parent=Msa0880780;Name=Msa0880780-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|176
chr6_1 exon 27626823 27627353 27626823 ID=Msa0880780-mRNA-1:exon:6803;Parent=Msa0880780-mRNA-1
chr6_1 CDS 27626823 27627353 27626823 ID=Msa0880780-mRNA-1:cds;Parent=Msa0880780-mRNA-1
Gene Sequence

>Msa0880780

ATGGTACTAGCGACATGCGCTGCCATATGTCGCCAGGTAGGATTACCGTTTCGTTATCCAGGAAATAGGTACACTTTGGAGCATTTCTGCGATATGACGGATGCTCGTGTGCTCGCAAAGCAGCATGTGTGGGCTGCAGTTACAAAGAAGGCCAAGAACCAAGCGTTTAATTGCACAAATGGTGACGTTTTCACATGGAAGAGTATGTGGAAGGTGCTTTGTAAGACTTTTGCTGTTAAGTTTGTGGAGTTGGATGAAAAGGAAGAGTTTGATTTGGTGCAGTTTATGCGTGATAAGGGTGAGATTTGGGATCAGATTGTGGAGGAGTATGGACTTCATAAGACCAAATTGGAGAAAATTGCTTGTTTTGAAGCCTTGGTGCCAGTTTTTCGATTTGAGTTTCAACTTGTATCTAGTATGAATAAGAGTAAAAATTATGAGTTTTTTGAGTATGCTGAGACGTTTAATAGTGTGAAATTCTGGGTGATGAAGCTGAGGGAGATGAATCTCATACCCATCTACGAACATTGA

Protein sequence

>Msa0880780

MVLATCAAICRQVGLPFRYPGNRYTLEHFCDMTDARVLAKQHVWAAVTKKAKNQAFNCTNGDVFTWKSMWKVLCKTFAVKFVELDEKEEFDLVQFMRDKGEIWDQIVEEYGLHKTKLEKIACFEALVPVFRFEFQLVSSMNKSKNYEFFEYAETFNSVKFWVMKLREMNLIPIYEH*