AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0482340


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 9 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTACATTGGTGCTTCTT+CGG 0.344526 3_3:+88782154 None:intergenic
AGCATGGAGGGTGGAATTCC+TGG 0.364129 3_3:-88782210 Msa0482340:CDS
ATGGGCAAGCGTAGCATGGA+GGG 0.553759 3_3:-88782222 Msa0482340:CDS
CGCGTCTAAAAGATGCTGAT+GGG 0.556259 3_3:-88782240 None:intergenic
ACGCGTCTAAAAGATGCTGA+TGG 0.561523 3_3:-88782241 None:intergenic
GCTGATGGGCAAGCGTAGCA+TGG 0.562091 3_3:-88782226 Msa0482340:CDS
GGCAAGCGTAGCATGGAGGG+TGG 0.638619 3_3:-88782219 Msa0482340:CDS
ACATTGGACACTGAATGACC+AGG 0.691085 3_3:+88782192 None:intergenic
GATGGGCAAGCGTAGCATGG+AGG 0.692312 3_3:-88782223 Msa0482340:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGTTTGAATATTAATTTTT+TGG - chr3_3:88782021-88782040 Msa0482340:intron 10.0%
!! TTATATTCTTAATGAAACTT+AGG - chr3_3:88781942-88781961 Msa0482340:CDS 15.0%
! GATAAAAACTACTCTTACAT+TGG + chr3_3:88781892-88781911 None:intergenic 25.0%
! TAAACTAACTACTCTTACAT+TGG + chr3_3:88781925-88781944 None:intergenic 25.0%
! CTGATTAAGAACTAAAATCA+AGG + chr3_3:88782113-88782132 None:intergenic 25.0%
!!! CTTGATTTTAGTTCTTAATC+AGG - chr3_3:88782111-88782130 Msa0482340:intron 25.0%
!!! TTTTAGTTCTTAATCAGGAA+AGG - chr3_3:88782116-88782135 Msa0482340:intron 25.0%
TCTTAATGAAACTTAGGATC+TGG - chr3_3:88781948-88781967 Msa0482340:intron 30.0%
CTTAATGAAACTTAGGATCT+GGG - chr3_3:88781949-88781968 Msa0482340:intron 30.0%
CATATAAAGCTAGTGTAAAC+TGG - chr3_3:88781987-88782006 Msa0482340:intron 30.0%
CATTGTTCGATCAAAAATCA+TGG - chr3_3:88782195-88782214 Msa0482340:CDS 30.0%
! CTCTTACATTGGTGCTTCTT+CGG + chr3_3:88781914-88781933 None:intergenic 40.0%
ACATTGGACACTGAATGACC+AGG + chr3_3:88781876-88781895 None:intergenic 45.0%
! TGAAACTTAGGATCTGGGAC+TGG - chr3_3:88781954-88781973 Msa0482340:intron 45.0%
ATGGGCAAGCGTAGCATGGA+GGG - chr3_3:88781843-88781862 Msa0482340:CDS 55.0%
AGCATGGAGGGTGGAATTCC+TGG - chr3_3:88781855-88781874 Msa0482340:CDS 55.0%
! GCTGATGGGCAAGCGTAGCA+TGG - chr3_3:88781839-88781858 Msa0482340:CDS 60.0%
GATGGGCAAGCGTAGCATGG+AGG - chr3_3:88781842-88781861 Msa0482340:CDS 60.0%
GGCAAGCGTAGCATGGAGGG+TGG - chr3_3:88781846-88781865 Msa0482340:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 88781837 88782250 88781837 ID=Msa0482340;Name=Msa0482340
chr3_3 mRNA 88781837 88782250 88781837 ID=Msa0482340-mRNA-1;Parent=Msa0482340;Name=Msa0482340-mRNA-1;_AED=0.70;_eAED=1.00;_QI=0|0|0|1|1|1|2|0|72
chr3_3 exon 88782161 88782250 88782161 ID=Msa0482340-mRNA-1:exon:26016;Parent=Msa0482340-mRNA-1
chr3_3 exon 88781837 88781965 88781837 ID=Msa0482340-mRNA-1:exon:26015;Parent=Msa0482340-mRNA-1
chr3_3 CDS 88782161 88782250 88782161 ID=Msa0482340-mRNA-1:cds;Parent=Msa0482340-mRNA-1
chr3_3 CDS 88781837 88781965 88781837 ID=Msa0482340-mRNA-1:cds;Parent=Msa0482340-mRNA-1
Gene Sequence

>Msa0482340

ATGCTGATGGGCAAGCGTAGCATGGAGGGTGGAATTCCTGGTCATTCAGTGTCCAATGTAAGAGTAGTTTTTATCCGAAGAAGCACCAATTTCTTAATCAGGAAAGGAAATGACTTCTCAAATACTTTCAAGTCAAGTAGAATCACAGTGCAATTTTATTCAACATTGTTCGATCAAAAATCATGGACACAAGTAAAATCCTCTCTTACTAATCAATAA

Protein sequence

>Msa0482340

MLMGKRSMEGGIPGHSVSNVRVVFIRRSTNFLIRKGNDFSNTFKSSRITVQFYSTLFDQKSWTQVKSSLTNQ*