AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1436570


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1436570 MtrunA17_Chr4g0068141 95.455 176 8 0 1 176 201 376 5.57e-126 358
Msa1436570 MtrunA17_Chr4g0068121 77.714 175 39 0 1 175 201 375 7.76e-103 300
Msa1436570 MtrunA17_Chr4g0036961 78.409 176 38 0 1 176 201 376 2.46e-102 299
Msa1436570 MtrunA17_Chr3g0082001 40.805 174 87 3 7 172 225 390 3.39e-42 145
Msa1436570 MtrunA17_Chr3g0134551 40.805 174 87 3 7 172 225 390 3.69e-42 145
Msa1436570 MtrunA17_Chr1g0152791 39.766 171 102 1 2 172 38 207 9.88e-42 139
Msa1436570 MtrunA17_Chr3g0134561 40.116 172 101 2 2 172 220 390 7.38e-41 141
Msa1436570 MtrunA17_Chr3g0134571 39.759 166 99 1 7 172 214 378 9.10e-40 138
Msa1436570 MtrunA17_Chr1g0152811 39.181 171 103 1 2 172 256 425 2.22e-39 138
Msa1436570 MtrunA17_Chr1g0152831 41.212 165 96 1 2 166 254 417 7.31e-39 138
Msa1436570 MtrunA17_Chr3g0104611 35.429 175 107 3 3 172 204 377 5.89e-30 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1436570 AT4G24220.1 35.673 171 109 1 2 172 219 388 3.16e-36 129
Msa1436570 AT4G24220.2 35.673 171 109 1 2 172 218 387 3.96e-36 129
Msa1436570 AT5G58750.2 32.571 175 114 3 3 173 204 378 1.58e-23 95.9
Msa1436570 AT5G58750.1 32.571 175 114 3 3 173 212 386 1.69e-23 95.9

Find 34 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTTAATAGTGTGAAATTC+TGG 0.190417 tig0027944:+23168 Msa1436570:CDS
CGTTTCGTTATCCAGGAAAT+AGG 0.260788 tig0027944:+22754 Msa1436570:CDS
TGCGTGATAAGGGTGAGATT+TGG 0.302571 tig0027944:+22994 Msa1436570:CDS
GCAATTAAACGCTTGGTTCT+TGG 0.326986 tig0027944:-22859 None:intergenic
GCGTGATAAGGGTGAGATTT+GGG 0.351508 tig0027944:+22995 Msa1436570:CDS
TGTAGTTCAATGTTCGTAGA+TGG 0.355494 tig0027944:-23222 None:intergenic
TGCTGTTAAGTTTGTGGAGT+TGG 0.382859 tig0027944:+22938 Msa1436570:CDS
TGGACTTCATAAGACCAAAT+TGG 0.385145 tig0027944:+23037 Msa1436570:CDS
GATCAGATTGTGGAGGAGTA+TGG 0.407176 tig0027944:+23017 Msa1436570:CDS
CAAGCGTTTAATTGCACAAA+TGG 0.411281 tig0027944:+22867 Msa1436570:CDS
TGAAAAGGAAGAGTTTGATT+TGG 0.422569 tig0027944:+22962 Msa1436570:CDS
TCCAAAGTGTACCTATTTCC+TGG 0.427390 tig0027944:-22765 None:intergenic
GGATTACCGTTTCGTTATCC+AGG 0.443194 tig0027944:+22747 Msa1436570:CDS
GTTTGTGGAGTTGGATGAAA+AGG 0.451890 tig0027944:+22947 Msa1436570:CDS
GGTGCAGTTTATGCGTGATA+AGG 0.456957 tig0027944:+22983 Msa1436570:CDS
GTGCAGTTTATGCGTGATAA+GGG 0.465205 tig0027944:+22984 Msa1436570:CDS
TAATCCTACCTGGCGACATA+TGG 0.474335 tig0027944:-22730 None:intergenic
CATTTGTGCAATTAAACGCT+TGG 0.482702 tig0027944:-22866 None:intergenic
TCCAGGAAATAGGTACACTT+TGG 0.499854 tig0027944:+22764 Msa1436570:CDS
GTTTAATAGTGTGAAATTCT+GGG 0.501763 tig0027944:+23169 Msa1436570:CDS
TGAGATTTGGGATCAGATTG+TGG 0.512590 tig0027944:+23007 Msa1436570:CDS
TGCTCGCAAAGCAGCATGTG+TGG 0.516452 tig0027944:+22817 Msa1436570:CDS
CTATTTCCTGGATAACGAAA+CGG 0.534217 tig0027944:-22753 None:intergenic
ATGCGCTGCCATATGTCGCC+AGG 0.550376 tig0027944:+22722 Msa1436570:CDS
GGAGCATTTCTGCGATATGA+CGG 0.555683 tig0027944:+22785 Msa1436570:CDS
GTAGTTCAATGTTCGTAGAT+GGG 0.556971 tig0027944:-23221 None:intergenic
GTGGGCTGCAGTTACAAAGA+AGG 0.580004 tig0027944:+22836 Msa1436570:CDS
CACATGGAAGAGTATGTGGA+AGG 0.602569 tig0027944:+22899 Msa1436570:CDS
GCTCGCAAAGCAGCATGTGT+GGG 0.604913 tig0027944:+22818 Msa1436570:CDS
AACGAAACGGTAATCCTACC+TGG 0.609340 tig0027944:-22740 None:intergenic
AATTCTGGGTGATGAAGCTG+AGG 0.665041 tig0027944:+23183 Msa1436570:CDS
GCTGCCATATGTCGCCAGGT+AGG 0.675590 tig0027944:+22726 Msa1436570:CDS
GATTTGGGATCAGATTGTGG+AGG 0.682099 tig0027944:+23010 Msa1436570:CDS
ATTCTGGGTGATGAAGCTGA+GGG 0.787809 tig0027944:+23184 Msa1436570:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GTTTAATAGTGTGAAATTCT+GGG + tig0027944:23169-23188 Msa1436570:CDS 25.0%
TGAAAAGGAAGAGTTTGATT+TGG + tig0027944:22962-22981 Msa1436570:CDS 30.0%
! ACAAGCAATTTTCTCCAATT+TGG - tig0027944:23054-23073 None:intergenic 30.0%
TGAAACTCAAATCGAAAAAC+TGG - tig0027944:23092-23111 None:intergenic 30.0%
CGTTTAATAGTGTGAAATTC+TGG + tig0027944:23168-23187 Msa1436570:CDS 30.0%
CTATTTCCTGGATAACGAAA+CGG - tig0027944:22756-22775 None:intergenic 35.0%
CATTTGTGCAATTAAACGCT+TGG - tig0027944:22869-22888 None:intergenic 35.0%
CAAGCGTTTAATTGCACAAA+TGG + tig0027944:22867-22886 Msa1436570:CDS 35.0%
!! TTTTCACATGGAAGAGTATG+TGG + tig0027944:22895-22914 Msa1436570:CDS 35.0%
!!! GACTTTTGCTGTTAAGTTTG+TGG + tig0027944:22932-22951 Msa1436570:CDS 35.0%
TGGACTTCATAAGACCAAAT+TGG + tig0027944:23037-23056 Msa1436570:CDS 35.0%
!!! AATTGCTTGTTTTGAAGCCT+TGG + tig0027944:23064-23083 Msa1436570:CDS 35.0%
CGTTTCGTTATCCAGGAAAT+AGG + tig0027944:22754-22773 Msa1436570:CDS 40.0%
TCCAGGAAATAGGTACACTT+TGG + tig0027944:22764-22783 Msa1436570:CDS 40.0%
TCCAAAGTGTACCTATTTCC+TGG - tig0027944:22768-22787 None:intergenic 40.0%
! GCAATTAAACGCTTGGTTCT+TGG - tig0027944:22862-22881 None:intergenic 40.0%
!! CAAATGGTGACGTTTTCACA+TGG + tig0027944:22883-22902 Msa1436570:CDS 40.0%
TGCTGTTAAGTTTGTGGAGT+TGG + tig0027944:22938-22957 Msa1436570:CDS 40.0%
GTTTGTGGAGTTGGATGAAA+AGG + tig0027944:22947-22966 Msa1436570:CDS 40.0%
GTGCAGTTTATGCGTGATAA+GGG + tig0027944:22984-23003 Msa1436570:CDS 40.0%
! TGAGATTTGGGATCAGATTG+TGG + tig0027944:23007-23026 Msa1436570:CDS 40.0%
AAATCGAAAAACTGGCACCA+AGG - tig0027944:23084-23103 None:intergenic 40.0%
TAATCCTACCTGGCGACATA+TGG - tig0027944:22733-22752 None:intergenic 45.0%
AACGAAACGGTAATCCTACC+TGG - tig0027944:22743-22762 None:intergenic 45.0%
GGATTACCGTTTCGTTATCC+AGG + tig0027944:22747-22766 Msa1436570:CDS 45.0%
! GGAGCATTTCTGCGATATGA+CGG + tig0027944:22785-22804 Msa1436570:CDS 45.0%
! CACATGGAAGAGTATGTGGA+AGG + tig0027944:22899-22918 Msa1436570:CDS 45.0%
GGTGCAGTTTATGCGTGATA+AGG + tig0027944:22983-23002 Msa1436570:CDS 45.0%
TGCGTGATAAGGGTGAGATT+TGG + tig0027944:22994-23013 Msa1436570:CDS 45.0%
GCGTGATAAGGGTGAGATTT+GGG + tig0027944:22995-23014 Msa1436570:CDS 45.0%
! GATTTGGGATCAGATTGTGG+AGG + tig0027944:23010-23029 Msa1436570:CDS 45.0%
GATCAGATTGTGGAGGAGTA+TGG + tig0027944:23017-23036 Msa1436570:CDS 45.0%
AATTCTGGGTGATGAAGCTG+AGG + tig0027944:23183-23202 Msa1436570:CDS 45.0%
ATTCTGGGTGATGAAGCTGA+GGG + tig0027944:23184-23203 Msa1436570:CDS 45.0%
! GTGGGCTGCAGTTACAAAGA+AGG + tig0027944:22836-22855 Msa1436570:CDS 50.0%
TGCTCGCAAAGCAGCATGTG+TGG + tig0027944:22817-22836 Msa1436570:CDS 55.0%
GCTCGCAAAGCAGCATGTGT+GGG + tig0027944:22818-22837 Msa1436570:CDS 55.0%
ATGCGCTGCCATATGTCGCC+AGG + tig0027944:22722-22741 Msa1436570:CDS 60.0%
GCTGCCATATGTCGCCAGGT+AGG + tig0027944:22726-22745 Msa1436570:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0027944 gene 22708 23238 22708 ID=Msa1436570;Name=Msa1436570
tig0027944 mRNA 22708 23238 22708 ID=Msa1436570-mRNA-1;Parent=Msa1436570;Name=Msa1436570-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|176
tig0027944 exon 22708 23238 22708 ID=Msa1436570-mRNA-1:exon:10289;Parent=Msa1436570-mRNA-1
tig0027944 CDS 22708 23238 22708 ID=Msa1436570-mRNA-1:cds;Parent=Msa1436570-mRNA-1
Gene Sequence

>Msa1436570

ATGGTACTAGCGACATGCGCTGCCATATGTCGCCAGGTAGGATTACCGTTTCGTTATCCAGGAAATAGGTACACTTTGGAGCATTTCTGCGATATGACGGATGCTCGTGTGCTCGCAAAGCAGCATGTGTGGGCTGCAGTTACAAAGAAGGCCAAGAACCAAGCGTTTAATTGCACAAATGGTGACGTTTTCACATGGAAGAGTATGTGGAAGGTGCTTTGTAAGACTTTTGCTGTTAAGTTTGTGGAGTTGGATGAAAAGGAAGAGTTTGATTTGGTGCAGTTTATGCGTGATAAGGGTGAGATTTGGGATCAGATTGTGGAGGAGTATGGACTTCATAAGACCAAATTGGAGAAAATTGCTTGTTTTGAAGCCTTGGTGCCAGTTTTTCGATTTGAGTTTCAACTTGTATCTAGTATGAATAAGAGTAAAAATTATGAGTTTTTTGAGTATGCTGAGACGTTTAATAGTGTGAAATTCTGGGTGATGAAGCTGAGGGAGATGAATCTCATACCCATCTACGAACATTGA

Protein sequence

>Msa1436570

MVLATCAAICRQVGLPFRYPGNRYTLEHFCDMTDARVLAKQHVWAAVTKKAKNQAFNCTNGDVFTWKSMWKVLCKTFAVKFVELDEKEEFDLVQFMRDKGEIWDQIVEEYGLHKTKLEKIACFEALVPVFRFEFQLVSSMNKSKNYEFFEYAETFNSVKFWVMKLREMNLIPIYEH*