AlfalfaGEDB | Msa0936480

M. sativa cultivar zhongmu-4 / Msa0936480

KEGG: K00472
GO: GO:0003674 GO:0003824 GO:0004656 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005788 GO:0006464 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009790 GO:0009792 GO:0009987 GO:0010171 GO:0010172 GO:0012505 GO:0016491 GO:0016705 GO:0016706 GO:0018126 GO:0018193 GO:0018208 GO:0018996 GO:0019511 GO:0019538 GO:0019798 GO:0031543 GO:0031545 GO:0031974 GO:0032501 GO:0032502 GO:0036211 GO:0040002 GO:0040008 GO:0040014 GO:0040018 GO:0042303 GO:0042335 GO:0042338 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045927 GO:0048518 GO:0048598 GO:0048638 GO:0048639 GO:0048856 GO:0050789 GO:0050793 GO:0051094 GO:0051213 GO:0051239 GO:0051240 GO:0055114 GO:0065007 GO:0070013 GO:0071704 GO:0140096 GO:1901564
PFAM: PF13640.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0936480	sp\|Q8L970\|P4H7_ARATH	56.825	315	89	4	6	277	6	316	5.74e-124	358

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0936480	B7FKS2	83.492	315	12	4	1	277	1	313	0.0	510

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0008200	Msa0936480	0.825744	3.966907e-54	-8.615850e-47
Msa0010490	Msa0936480	0.821415	4.087359e-53	-8.615850e-47
Msa0010500	Msa0936480	0.819082	1.399760e-52	-8.615850e-47
Msa0020390	Msa0936480	0.807094	5.979564e-50	-8.615850e-47
Msa0024400	Msa0936480	0.807445	5.038036e-50	-8.615850e-47
Msa0027890	Msa0936480	0.800151	1.648504e-48	-8.615850e-47
Msa0038870	Msa0936480	0.804074	2.572420e-49	-8.615850e-47
Msa0068020	Msa0936480	0.820582	6.356940e-53	-8.615850e-47
Msa0072420	Msa0936480	0.812248	4.663528e-51	-8.615850e-47
Msa0090520	Msa0936480	0.833251	5.935628e-56	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0936480	MtrunA17_Chr6g0452281	83.492	315	12	4	1	277	1	313	0.0	510
Msa0936480	MtrunA17_Chr2g0286811	53.901	282	86	3	34	275	26	303	2.67e-103	303
Msa0936480	MtrunA17_Chr4g0066531	52.768	271	86	3	43	275	32	298	6.54e-97	286
Msa0936480	MtrunA17_Chr3g0143381	45.276	254	102	3	6	222	26	279	8.57e-71	219
Msa0936480	MtrunA17_Chr5g0406051	48.341	211	73	2	48	222	78	288	9.03e-66	207
Msa0936480	MtrunA17_Chr6g0470551	38.411	302	147	6	6	275	3	297	2.12e-65	206
Msa0936480	MtrunA17_Chr8g0371281	46.218	238	88	4	21	221	41	275	3.51e-65	205
Msa0936480	MtrunA17_Chr8g0371221	46.218	238	89	4	21	221	41	276	4.25e-64	202
Msa0936480	MtrunA17_Chr8g0371251	43.697	238	98	2	21	222	41	278	5.11e-63	199
Msa0936480	MtrunA17_Chr8g0371231	53.793	145	66	1	76	219	55	199	1.53e-51	167

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0936480	AT3G28480.1	56.825	315	89	4	6	277	6	316	5.83e-125	358
Msa0936480	AT3G28480.2	55.418	323	89	4	6	277	6	324	9.06e-124	356
Msa0936480	AT3G28490.1	58.582	268	68	2	44	275	28	288	4.10e-112	325
Msa0936480	AT5G18900.1	52.015	273	89	3	41	275	30	298	1.79e-94	281
Msa0936480	AT3G06300.1	53.184	267	83	3	47	275	37	299	1.36e-93	278
Msa0936480	AT4G25600.1	45.946	259	106	7	36	275	48	291	5.93e-72	223
Msa0936480	AT1G20270.1	49.519	208	69	2	51	222	79	286	7.64e-65	204
Msa0936480	AT2G17720.1	47.867	211	74	2	48	222	80	290	2.80e-63	201
Msa0936480	AT4G25600.3	44.351	239	100	6	56	275	6	230	3.21e-63	199
Msa0936480	AT4G25600.2	44.351	239	100	6	56	275	15	239	3.73e-63	199

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Find 64 sgRNAs with CRISPR-Local

Find 332 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATAAGAGAGATTAGAGAG+AGG	0.547974	6_3:+7221705	None:intergenic
AACAAGGCGTCACCCTTCCG+AGG	0.602551	6_3:+7217362	None:intergenic
AACTGAATAGAAATGAAGAA+AGG	0.546047	6_3:-7216980	Msa0936480:three_prime_UTR
AAGAAAGGAGTTCTCCTCTA+GGG	0.542203	6_3:-7216965	Msa0936480:three_prime_UTR
AAGGAGAGATTTATTTGTAA+AGG	0.449994	6_3:+7216930	None:intergenic
AAGGCTAATCAACGACTTGG+CGG	0.579353	6_3:-7218260	Msa0936480:CDS
AATAAGAGAGATTAGAGAGA+GGG	0.438010	6_3:+7221706	None:intergenic
AATGTTAATTAAATGTTAAT+TGG	0.206561	6_3:+7221758	None:intergenic
AATGTTTGCTCTTCTTAGAG+AGG	0.567861	6_3:-7217071	Msa0936480:exon
ACAATGGAAGAAAACGAGGT+TGG	0.559292	6_3:+7221602	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATAAGAGAGATTAGAGAGA+GGG	+	chr6_3:7217010-7217029	None:intergenic	30.0%
AAATAAGAGAGATTAGAGAG+AGG	+	chr6_3:7217011-7217030	None:intergenic	30.0%
TCGATAACGTGTTTATAAGT+CGG	-	chr6_3:7217693-7217712	Msa0936480:intron	30.0%
TATAGGTTTGAATTAGGCTA+TGG	-	chr6_3:7217850-7217869	Msa0936480:intron	30.0%
TTGAATTAGGCTATGGTATT+TGG	-	chr6_3:7217857-7217876	Msa0936480:intron	30.0%
ATTCCTTCACAAATTTCCTA+TGG	-	chr6_3:7217982-7218001	Msa0936480:intron	30.0%
AAAATCGGTGTGTAAAGTAA+GGG	+	chr6_3:7218105-7218124	None:intergenic	30.0%
ATTCCTTCACAAATTTCCTA+TGG	-	chr6_3:7217982-7218001	Msa0936480:intron	30.0%
CCCTCTTATAAACTAATTTC+CGG	+	chr6_3:7218710-7218729	None:intergenic	30.0%
ATATAACAAGAACCATGTGT+TGG	+	chr6_3:7219177-7219196	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_3	gene	7216887	7221848	7216887	ID=Msa0936480;Name=Msa0936480
chr6_3	mRNA	7216887	7221848	7216887	ID=Msa0936480-mRNA-1;Parent=Msa0936480;Name=Msa0936480-mRNA-1;_AED=0.15;_eAED=0.15;_QI=219\|0.83\|1\|1\|1\|1\|7\|189\|277
chr6_3	exon	7216887	7217389	7216887	ID=Msa0936480-mRNA-1:exon:2369;Parent=Msa0936480-mRNA-1
chr6_3	exon	7218023	7218083	7218023	ID=Msa0936480-mRNA-1:exon:2368;Parent=Msa0936480-mRNA-1
chr6_3	exon	7218184	7218359	7218184	ID=Msa0936480-mRNA-1:exon:2367;Parent=Msa0936480-mRNA-1
chr6_3	exon	7218444	7218501	7218444	ID=Msa0936480-mRNA-1:exon:2366;Parent=Msa0936480-mRNA-1
chr6_3	exon	7221195	7221252	7221195	ID=Msa0936480-mRNA-1:exon:2365;Parent=Msa0936480-mRNA-1
chr6_3	exon	7221358	7221429	7221358	ID=Msa0936480-mRNA-1:exon:2364;Parent=Msa0936480-mRNA-1
chr6_3	exon	7221535	7221848	7221535	ID=Msa0936480-mRNA-1:exon:2363;Parent=Msa0936480-mRNA-1
chr6_3	five_prime_UTR	7221630	7221848	7221630	ID=Msa0936480-mRNA-1:five_prime_utr;Parent=Msa0936480-mRNA-1
chr6_3	CDS	7221535	7221629	7221535	ID=Msa0936480-mRNA-1:cds;Parent=Msa0936480-mRNA-1
chr6_3	CDS	7221358	7221429	7221358	ID=Msa0936480-mRNA-1:cds;Parent=Msa0936480-mRNA-1
chr6_3	CDS	7221195	7221252	7221195	ID=Msa0936480-mRNA-1:cds;Parent=Msa0936480-mRNA-1
chr6_3	CDS	7218444	7218501	7218444	ID=Msa0936480-mRNA-1:cds;Parent=Msa0936480-mRNA-1
chr6_3	CDS	7218184	7218359	7218184	ID=Msa0936480-mRNA-1:cds;Parent=Msa0936480-mRNA-1
chr6_3	CDS	7218023	7218083	7218023	ID=Msa0936480-mRNA-1:cds;Parent=Msa0936480-mRNA-1
chr6_3	CDS	7217076	7217389	7217076	ID=Msa0936480-mRNA-1:cds;Parent=Msa0936480-mRNA-1
chr6_3	three_prime_UTR	7216887	7217075	7216887	ID=Msa0936480-mRNA-1:three_prime_utr;Parent=Msa0936480-mRNA-1

Gene Sequence

>Msa0936480

ATGTTCCAACCTCGTTTTCTTCCATTGTTACTCTGTTTTTTCTTCTCTGTTTCTTCAATTCGATTACCTGGTTTGGAAGAAGAAAACAAAATCACGCGTGGATCGGTTTTTGGAGCAAAAGTCAAGTTTGATCCGACACGTGTTACTCAGCTCTCGTGGAGTCCTAGGGCTTTTCTGTATAAGAATTTTTTGACTGATGAAGAATGTGATCATCTTATTGAATTGGATGAAATAGTTTCTGGTATTGAAGCACGAATTGCCGCATGGACATTCCTTCCCGTAGAGAATGGTGAGTCTATGCAAGTATTGCACTACATGAACGGCGAGAAATACGAACCACATTTTGATTTCTTCCACGACAAGGCTAATCAACGACTTGGCGGCCATCGTGTTGCAACGGTGCTGATGTATTTGTCTAATGTTGAGAAGGGTGGAGAAACCATTTTTCCTCATGCCGAGGGGAGGTCGTCGCAGCCAAAAGATGAGAGCTGGTCAGAATGTGCTCACAAAGGATATGCAGTAAAACCTCGGAAGGGTGACGCCTTGTTGTTCTTCAGTCTTCATCTAGATGCAACCACAGATTCAAAAAGCTTGCATGGAAGCTGTCCAGTGATTGAAGGTGAGAAGTGGTCAGCAACAAAGTGGATTCATGTTGCCGACTTTGAGAAACCGATACGACAAGCATTGGAGGATGATGGAGAGTGTGTTGATGAGAATGAGAATTGTGCTAGGTGGGCTAAAGTTGGCGAATGTGAGAAAAATCCACGTTATATGGTTGGTAATGGAGGAGTTAAAGGAAAATGTATGAAGAGTTGCAATGTTTGCTCTTCTTAG

Protein sequence

>Msa0936480

MFQPRFLPLLLCFFFSVSSIRLPGLEEENKITRGSVFGAKVKFDPTRVTQLSWSPRAFLYKNFLTDEECDHLIELDEIVSGIEARIAAWTFLPVENGESMQVLHYMNGEKYEPHFDFFHDKANQRLGGHRVATVLMYLSNVEKGGETIFPHAEGRSSQPKDESWSECAHKGYAVKPRKGDALLFFSLHLDATTDSKSLHGSCPVIEGEKWSATKWIHVADFEKPIRQALEDDGECVDENENCARWAKVGECEKNPRYMVGNGGVKGKCMKSCNVCSS*