AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1333890

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 65 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGATTATTGCTTGAGTATT+TGG 0.102072 8_4:-54157424 None:intergenic
TCAGAGAGAATTAGGAAATT+TGG 0.161809 8_4:-54156938 None:intergenic
TTTAAAACTTCTGCTATAAA+AGG 0.186926 8_4:+54158815 Msa1333890:three_prime_UTR
AGAGGGTCTGCACCTCTTTA+TGG 0.203404 8_4:+54156915 Msa1333890:five_prime_UTR
TGCAGCATCTTTCTGTTGTT+GGG 0.217013 8_4:-54156886 None:intergenic
TTGAAACTTCTCATTATATT+TGG 0.217769 8_4:-54156809 None:intergenic
CTGCAGCATCTTTCTGTTGT+TGG 0.236722 8_4:-54156887 None:intergenic
CTAAAAGAGAAATTCATCTT+TGG 0.284907 8_4:-54157154 None:intergenic
AAATAATCAAAGACAGTAAA+AGG 0.296177 8_4:-54157367 None:intergenic
TTTCAGGAAGAAGAACTAAA+AGG 0.299609 8_4:+54158468 Msa1333890:intron
AAATTTACTACATATATATC+AGG 0.318147 8_4:-54157338 None:intergenic
GGAGGAGTTCCATGCAATTC+TGG 0.326352 8_4:+54157489 Msa1333890:three_prime_UTR
CGAGATGGTCAGAAGAAATA+AGG 0.350306 8_4:-54157013 None:intergenic
AAAGATGCTGCAGAACTTAG+AGG 0.374502 8_4:+54156897 Msa1333890:five_prime_UTR
GGAAAGTAACACCTCCAATA+TGG 0.374802 8_4:-54157403 None:intergenic
ATCTTGTGGTTTGGTCCACC+TGG 0.375322 8_4:-54157299 None:intergenic
AATTCTCTCTGAAAATGATA+AGG 0.403573 8_4:+54156949 Msa1333890:five_prime_UTR
TCCCACATTTACAAGTGAAA+TGG 0.405861 8_4:-54157220 None:intergenic
ACAATCTCATGTGATATTGT+AGG 0.413476 8_4:+54156779 Msa1333890:five_prime_UTR
GAGAGACTTAGGTTAGTTGC+TGG 0.418417 8_4:-54157038 None:intergenic
TCATGTGATATTGTAGGTTA+AGG 0.422723 8_4:+54156785 Msa1333890:five_prime_UTR
TTGATTCAAGTGACACTGAT+AGG 0.424944 8_4:+54157452 Msa1333890:three_prime_UTR
ATTCTCTCTGAAAATGATAA+GGG 0.430279 8_4:+54156950 Msa1333890:five_prime_UTR
CTGATAGGCTTGTGATAGCT+AGG 0.436345 8_4:+54157467 Msa1333890:three_prime_UTR
AAAATGAATAAGATTGCGTT+AGG 0.452224 8_4:-54157086 None:intergenic
TAGTAATTTATTTATAATGT+TGG 0.453327 8_4:+54157174 Msa1333890:five_prime_UTR
CTTATTTCTTCTGACCATCT+CGG 0.455218 8_4:+54157014 Msa1333890:five_prime_UTR
GCAGCATCTTTCTGTTGTTG+GGG 0.465239 8_4:-54156885 None:intergenic
TAAGTGAGAGTGAGAGACTT+AGG 0.467222 8_4:-54157049 None:intergenic
GAACGTTGCCTATAAACAAA+AGG 0.499568 8_4:-54156991 None:intergenic
GGCATGTCAGAACTACTGCT+TGG 0.499951 8_4:-54157199 None:intergenic
TGCCATTTCACTTGTAAATG+TGG 0.503421 8_4:+54157218 Msa1333890:five_prime_UTR
GCATGTCAGAACTACTGCTT+GGG 0.508472 8_4:-54157198 None:intergenic
TAGGAAATTTGGCCATAAAG+AGG 0.518086 8_4:-54156927 None:intergenic
TTGTGGAAAGAAATGCTTTG+TGG 0.518926 8_4:+54157129 Msa1333890:five_prime_UTR
TGGTCTAGGAAATATGCCTA+TGG 0.520014 8_4:+54159937 Msa1333890:three_prime_UTR
AACACCTCCAATATGGCCTA+TGG 0.520360 8_4:-54157396 None:intergenic
ATGTGAAGGGAGGTATATCT+TGG 0.522728 8_4:+54159917 Msa1333890:intron
CAGCATCTTTCTGTTGTTGG+GGG 0.523423 8_4:-54156884 None:intergenic
AGGGAGGTATATCTTGGTCT+AGG 0.526837 8_4:+54159923 Msa1333890:intron
TTGTCCATAGGCCATATTGG+AGG 0.528609 8_4:+54157392 Msa1333890:CDS
CCGTCACTGCAGCATCATCA+AGG 0.530798 8_4:-54158748 None:intergenic
CCTTGATGATGCTGCAGTGA+CGG 0.531460 8_4:+54158748 Msa1333890:three_prime_UTR
GCCATTTCACTTGTAAATGT+GGG 0.533467 8_4:+54157219 Msa1333890:five_prime_UTR
TCCATTATGCAGGATCTACC+AGG 0.537332 8_4:+54158722 Msa1333890:intron
GCAGCATCATCAAGGGCGCC+TGG 0.541314 8_4:-54158740 None:intergenic
AGGTGGACCAAACCACAAGA+TGG 0.559402 8_4:+54157301 Msa1333890:exon
GGTTAGTTGCTGGACCGAGA+TGG 0.572718 8_4:-54157028 None:intergenic
CGTTGCCTATAAACAAAAGG+AGG 0.572953 8_4:-54156988 None:intergenic
ACTTCTGCTATAAAAGGCGA+AGG 0.577101 8_4:+54158821 Msa1333890:three_prime_UTR
CGTCACTGCAGCATCATCAA+GGG 0.584247 8_4:-54158747 None:intergenic
CACATACCTCCAGAATTGCA+TGG 0.589186 8_4:-54157498 None:intergenic
GGTGGACCAAACCACAAGAT+GGG 0.595478 8_4:+54157302 Msa1333890:exon
AAGATGCTGCAGAACTTAGA+GGG 0.603214 8_4:+54156898 Msa1333890:five_prime_UTR
AATTTACTACATATATATCA+GGG 0.607912 8_4:-54157337 None:intergenic
TGATAGGCTTGTGATAGCTA+GGG 0.616455 8_4:+54157468 Msa1333890:three_prime_UTR
TGATTAAAAGAACAAATCAC+AGG 0.621034 8_4:+54157265 Msa1333890:five_prime_UTR
AACTGCGGTAGCTCATATTG+TGG 0.625455 8_4:+54157112 Msa1333890:five_prime_UTR
TCACAGGAAAATCGAAAACC+AGG 0.635015 8_4:+54157281 Msa1333890:five_prime_UTR
GTTGCCTATAAACAAAAGGA+GGG 0.638555 8_4:-54156987 None:intergenic
TACACAACGTAAAAGTCCAT+AGG 0.645551 8_4:-54159953 None:intergenic
TAGGCTTGTGATAGCTAGGG+AGG 0.666014 8_4:+54157471 Msa1333890:three_prime_UTR
GGAGTTCCATGCAATTCTGG+AGG 0.674172 8_4:+54157492 Msa1333890:three_prime_UTR
TTGCCTATAAACAAAAGGAG+GGG 0.717256 8_4:-54156986 None:intergenic
CAGGAAAATCGAAAACCAGG+TGG 0.722415 8_4:+54157284 Msa1333890:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGTAATTTATTTATAATGT+TGG + chr8_4:54157174-54157193 Msa1333890:five_prime_UTR 10.0%
!! AAAATAAAAACAATATTTAC+AGG - chr8_4:54159081-54159100 None:intergenic 10.0%
!!! TAAAAAGTTTTTCAAATATA+AGG - chr8_4:54159559-54159578 None:intergenic 10.0%
!!! AAAAACTTTTTACAAAAAAT+GGG + chr8_4:54159567-54159586 Msa1333890:intron 10.0%
!!! AAATTTTCATAAAATTTATG+CGG + chr8_4:54159648-54159667 Msa1333890:intron 10.0%
!!! TTTTTTCCTAATAAAATACA+AGG - chr8_4:54156859-54156878 None:intergenic 15.0%
!! AATTTACTACATATATATCA+GGG - chr8_4:54157340-54157359 None:intergenic 15.0%
!! AAATTTACTACATATATATC+AGG - chr8_4:54157341-54157360 None:intergenic 15.0%
!! AATTATTTATCAAACAAAAC+AGG - chr8_4:54157926-54157945 None:intergenic 15.0%
!! TATAATTACCAGTAAAAATA+AGG - chr8_4:54157964-54157983 None:intergenic 15.0%
!! CAATAAAATTACAATTACTA+AGG - chr8_4:54158175-54158194 None:intergenic 15.0%
!!! TAGTAATTGTAATTTTATTG+TGG + chr8_4:54158175-54158194 Msa1333890:intron 15.0%
!! TTAAAATAAAATCTAGTCAA+AGG - chr8_4:54158351-54158370 None:intergenic 15.0%
!!! TTTCATTTTTCAGTATTTAA+AGG + chr8_4:54158956-54158975 Msa1333890:intron 15.0%
!!! TAATGTTTAGCAATTTTTAT+AGG + chr8_4:54159188-54159207 Msa1333890:intron 15.0%
!! GATATTAAAATTATCTGTTA+TGG - chr8_4:54159248-54159267 None:intergenic 15.0%
!!! TTTGACTTTAAAGTTTTTAT+AGG + chr8_4:54159467-54159486 Msa1333890:intron 15.0%
!!! GAAAAACTTTTTACAAAAAA+TGG + chr8_4:54159566-54159585 Msa1333890:intron 15.0%
!!! AATTTTCATAAAATTTATGC+GGG + chr8_4:54159649-54159668 Msa1333890:intron 15.0%
!!! TTTGTTTGAGATATAAAAAA+GGG - chr8_4:54159723-54159742 None:intergenic 15.0%
!!! TTTTGTTTGAGATATAAAAA+AGG - chr8_4:54159724-54159743 None:intergenic 15.0%
!! TATATCTCAAACAAAATTTA+AGG + chr8_4:54159728-54159747 Msa1333890:intron 15.0%
!! ATATCTCAAACAAAATTTAA+GGG + chr8_4:54159729-54159748 Msa1333890:intron 15.0%
!!! GTTTTATTCTTCTAGATTTT+AGG - chr8_4:54156732-54156751 None:intergenic 20.0%
!! TTGAAACTTCTCATTATATT+TGG - chr8_4:54156812-54156831 None:intergenic 20.0%
!!! AAGAAACCTTGTATTTTATT+AGG + chr8_4:54156850-54156869 Msa1333890:five_prime_UTR 20.0%
!! AATGTGGGAAAAAAATTATA+TGG + chr8_4:54157234-54157253 Msa1333890:five_prime_UTR 20.0%
!! AAATAATCAAAGACAGTAAA+AGG - chr8_4:54157370-54157389 None:intergenic 20.0%
!!! TTGATTATTTTTTTGTCCAT+AGG + chr8_4:54157380-54157399 Msa1333890:CDS 20.0%
!!! TTTGTATTCCTTATTTTTAC+TGG + chr8_4:54157953-54157972 Msa1333890:intron 20.0%
!!! TAAAGGATTTGTTTACAAAA+TGG + chr8_4:54158019-54158038 Msa1333890:intron 20.0%
!!! ATTACTAAGGATTCATTTTT+TGG - chr8_4:54158162-54158181 None:intergenic 20.0%
!!! TAATTGTAATTTTATTGTGG+AGG + chr8_4:54158178-54158197 Msa1333890:intron 20.0%
!!! AATTGTAATTTTATTGTGGA+GGG + chr8_4:54158179-54158198 Msa1333890:intron 20.0%
!! GAAACGGATAAAAATTAATT+AGG - chr8_4:54158327-54158346 None:intergenic 20.0%
!!! AAGTTTTAAAAATAGACCAT+TGG - chr8_4:54158805-54158824 None:intergenic 20.0%
!!! TTTAAAACTTCTGCTATAAA+AGG + chr8_4:54158815-54158834 Msa1333890:three_prime_UTR 20.0%
!!! TAAAATTGAATGCTATTTCA+AGG + chr8_4:54158875-54158894 Msa1333890:intron 20.0%
!! TACAAATTTCTACTTATGTA+TGG - chr8_4:54159137-54159156 None:intergenic 20.0%
!! CAATTCATTTGATATAGTAT+AGG + chr8_4:54159283-54159302 Msa1333890:intron 20.0%
!! AATAAAAATAAAAAACGCAG+AGG + chr8_4:54159321-54159340 Msa1333890:intron 20.0%
!! ATAAAAATAAAAAACGCAGA+GGG + chr8_4:54159322-54159341 Msa1333890:intron 20.0%
!!! ATTTTCATAAAATTTATGCG+GGG + chr8_4:54159650-54159669 Msa1333890:intron 20.0%
!! GCAAAATGTAGAAATTATTA+TGG + chr8_4:54159834-54159853 Msa1333890:intron 20.0%
!! TATCATTTTCAGAGAGAATT+AGG - chr8_4:54156949-54156968 None:intergenic 25.0%
!! AATTCTCTCTGAAAATGATA+AGG + chr8_4:54156949-54156968 Msa1333890:five_prime_UTR 25.0%
!! ATTCTCTCTGAAAATGATAA+GGG + chr8_4:54156950-54156969 Msa1333890:five_prime_UTR 25.0%
! AAAATGAATAAGATTGCGTT+AGG - chr8_4:54157089-54157108 None:intergenic 25.0%
!!! CTTATTCATTTTTGAAACTG+CGG + chr8_4:54157097-54157116 Msa1333890:five_prime_UTR 25.0%
! CTAAAAGAGAAATTCATCTT+TGG - chr8_4:54157157-54157176 None:intergenic 25.0%
! TGATTAAAAGAACAAATCAC+AGG + chr8_4:54157265-54157284 Msa1333890:five_prime_UTR 25.0%
! TAGATTATTGCTTGAGTATT+TGG - chr8_4:54157427-54157446 None:intergenic 25.0%
! AGCTTATATTGCAATTTACA+AGG + chr8_4:54157613-54157632 Msa1333890:intron 25.0%
! GCTTATATTGCAATTTACAA+GGG + chr8_4:54157614-54157633 Msa1333890:intron 25.0%
!!! TTTTTGACTGAATCTAATTC+TGG + chr8_4:54157844-54157863 Msa1333890:intron 25.0%
! AAGTGTATTTACTGTTCTAA+AGG + chr8_4:54158002-54158021 Msa1333890:intron 25.0%
!! ATTGTAATTTTATTGTGGAG+GGG + chr8_4:54158180-54158199 Msa1333890:intron 25.0%
! AAGAAATGAAATTGACAAGT+AGG - chr8_4:54158246-54158265 None:intergenic 25.0%
! AGAATTTGATTCATAAGTGT+TGG + chr8_4:54158525-54158544 Msa1333890:intron 25.0%
! AAATTATCTGTTATGGATAG+TGG - chr8_4:54159241-54159260 None:intergenic 25.0%
!! TTTTTAATGGGAATTAGACT+TGG + chr8_4:54159394-54159413 Msa1333890:intron 25.0%
! ATAAGGACTAAACTTATGTA+GGG - chr8_4:54159542-54159561 None:intergenic 25.0%
! TATAAGGACTAAACTTATGT+AGG - chr8_4:54159543-54159562 None:intergenic 25.0%
!!! TCTCTAATTTGCTTTTATAG+GGG - chr8_4:54159626-54159645 None:intergenic 25.0%
!!! GTCTCTAATTTGCTTTTATA+GGG - chr8_4:54159627-54159646 None:intergenic 25.0%
!!! TGTCTCTAATTTGCTTTTAT+AGG - chr8_4:54159628-54159647 None:intergenic 25.0%
!! TAAAATGCTGTGCTTTATTA+AGG + chr8_4:54159680-54159699 Msa1333890:intron 25.0%
! AAATCTCGTTCTATTACATA+GGG - chr8_4:54159874-54159893 None:intergenic 25.0%
! TAAATCTCGTTCTATTACAT+AGG - chr8_4:54159875-54159894 None:intergenic 25.0%
ACAATCTCATGTGATATTGT+AGG + chr8_4:54156779-54156798 Msa1333890:five_prime_UTR 30.0%
TCATGTGATATTGTAGGTTA+AGG + chr8_4:54156785-54156804 Msa1333890:five_prime_UTR 30.0%
TCAGAGAGAATTAGGAAATT+TGG - chr8_4:54156941-54156960 None:intergenic 30.0%
ACACACTTCATTTACAACTT+TGG + chr8_4:54157552-54157571 Msa1333890:intron 30.0%
TATTGCAATTTACAAGGGAT+TGG + chr8_4:54157619-54157638 Msa1333890:intron 30.0%
! AAGCTTCACGTAATCTATTT+TGG - chr8_4:54157742-54157761 None:intergenic 30.0%
GTACTATAGAAGAATGATGT+AGG + chr8_4:54157777-54157796 Msa1333890:intron 30.0%
!! TAGGTGTTGTTTGTGATTAT+AGG + chr8_4:54157796-54157815 Msa1333890:intron 30.0%
AAACTAATTCCTCTTAGCTT+AGG - chr8_4:54157827-54157846 None:intergenic 30.0%
TGATTCATCCTAATTGGAAT+AGG + chr8_4:54157871-54157890 Msa1333890:intron 30.0%
!!! TGATCGTTGTTTTGAATGAT+TGG + chr8_4:54157897-54157916 Msa1333890:intron 30.0%
!!! GATCGTTGTTTTGAATGATT+GGG + chr8_4:54157898-54157917 Msa1333890:intron 30.0%
!!! ATCGTTGTTTTGAATGATTG+GGG + chr8_4:54157899-54157918 Msa1333890:intron 30.0%
TCGGAAACAACTTAAAACTA+TGG + chr8_4:54158087-54158106 Msa1333890:intron 30.0%
AAATCTAGTCAAAGGAGAAA+CGG - chr8_4:54158343-54158362 None:intergenic 30.0%
TAACTATTCTCCTTTGACTA+TGG + chr8_4:54158368-54158387 Msa1333890:intron 30.0%
!!! TTTCTCTGAACCTTTTTTTC+AGG + chr8_4:54158452-54158471 Msa1333890:intron 30.0%
AGTTCTTCTTCCTGAAAAAA+AGG - chr8_4:54158465-54158484 None:intergenic 30.0%
TTTCAGGAAGAAGAACTAAA+AGG + chr8_4:54158468-54158487 Msa1333890:intron 30.0%
!! TCTCATTTTTGCAAACAAAC+AGG + chr8_4:54158500-54158519 Msa1333890:three_prime_UTR 30.0%
ACAAGATTAAAAATCGCCAA+TGG + chr8_4:54158786-54158805 Msa1333890:three_prime_UTR 30.0%
!! AATGCTATTTCAAGGTAGTT+AGG + chr8_4:54158883-54158902 Msa1333890:intron 30.0%
!! GAGTTGATGATTGATAACAT+TGG - chr8_4:54158991-54159010 None:intergenic 30.0%
TATCAATCATCAACTCAGTT+AGG + chr8_4:54158995-54159014 Msa1333890:intron 30.0%
!!! GTGGATACTAGTTTTGTTAA+TGG - chr8_4:54159222-54159241 None:intergenic 30.0%
!!! TGGTCGCTGTTTTTTTTAAT+GGG + chr8_4:54159382-54159401 Msa1333890:intron 30.0%
AAATATGTTTGTAGTCCATG+TGG + chr8_4:54159493-54159512 Msa1333890:intron 30.0%
TTTAAGGGCAGTTATTCTTA+TGG + chr8_4:54159744-54159763 Msa1333890:intron 30.0%
ATTTCAAACGAGCATAAGTA+AGG - chr8_4:54159899-54159918 None:intergenic 30.0%
TAGGAAATTTGGCCATAAAG+AGG - chr8_4:54156930-54156949 None:intergenic 35.0%
TTGCCTATAAACAAAAGGAG+GGG - chr8_4:54156989-54157008 None:intergenic 35.0%
GTTGCCTATAAACAAAAGGA+GGG - chr8_4:54156990-54157009 None:intergenic 35.0%
GAACGTTGCCTATAAACAAA+AGG - chr8_4:54156994-54157013 None:intergenic 35.0%
! CTTATTTCTTCTGACCATCT+CGG + chr8_4:54157014-54157033 Msa1333890:five_prime_UTR 35.0%
TTGTGGAAAGAAATGCTTTG+TGG + chr8_4:54157129-54157148 Msa1333890:five_prime_UTR 35.0%
! TGCCATTTCACTTGTAAATG+TGG + chr8_4:54157218-54157237 Msa1333890:five_prime_UTR 35.0%
GCCATTTCACTTGTAAATGT+GGG + chr8_4:54157219-54157238 Msa1333890:five_prime_UTR 35.0%
TCCCACATTTACAAGTGAAA+TGG - chr8_4:54157223-54157242 None:intergenic 35.0%
! TGAATTCTTTTCCCATCTTG+TGG - chr8_4:54157316-54157335 None:intergenic 35.0%
! TTTTTGTCCATAGGCCATAT+TGG + chr8_4:54157389-54157408 Msa1333890:CDS 35.0%
TTGATTCAAGTGACACTGAT+AGG + chr8_4:54157452-54157471 Msa1333890:three_prime_UTR 35.0%
!! CTTCACGTAATCTATTTTGG+TGG - chr8_4:54157739-54157758 None:intergenic 35.0%
GGTGTTTGATTCATCCTAAT+TGG + chr8_4:54157865-54157884 Msa1333890:intron 35.0%
GATCAAAGCCTATTCCAATT+AGG - chr8_4:54157882-54157901 None:intergenic 35.0%
!!! TCGTTGTTTTGAATGATTGG+GGG + chr8_4:54157900-54157919 Msa1333890:intron 35.0%
! TAAGTTGTTTCCGACTATCA+AGG - chr8_4:54158081-54158100 None:intergenic 35.0%
GACAACAAAACCATAGTCAA+AGG - chr8_4:54158381-54158400 None:intergenic 35.0%
! TGGATGCACATATTTTCACT+AGG + chr8_4:54158545-54158564 Msa1333890:intron 35.0%
! GGATGCACATATTTTCACTA+GGG + chr8_4:54158546-54158565 Msa1333890:intron 35.0%
! GATGCACATATTTTCACTAG+GGG + chr8_4:54158547-54158566 Msa1333890:intron 35.0%
! ATGCACATATTTTCACTAGG+GGG + chr8_4:54158548-54158567 Msa1333890:intron 35.0%
GTGCATGTGCATAGATAATA+TGG + chr8_4:54158637-54158656 Msa1333890:intron 35.0%
TGCAGCATCACTACTTATTT+AGG - chr8_4:54158920-54158939 None:intergenic 35.0%
CAGAGGGAAAAAAAAAGACT+TGG + chr8_4:54159338-54159357 Msa1333890:intron 35.0%
!!! GTGGTCGCTGTTTTTTTTAA+TGG + chr8_4:54159381-54159400 Msa1333890:intron 35.0%
TGAATTTCCAATCCAACTAG+TGG + chr8_4:54159431-54159450 Msa1333890:intron 35.0%
! TTACAAGTTGGAATTCCACA+TGG - chr8_4:54159511-54159530 None:intergenic 35.0%
AGGGACTAAACTTTACAAGT+TGG - chr8_4:54159523-54159542 None:intergenic 35.0%
TGCACAGTCATACTTATGTA+AGG - chr8_4:54159773-54159792 None:intergenic 35.0%
! TATGCTCGTTTGAAATGTGA+AGG + chr8_4:54159903-54159922 Msa1333890:intron 35.0%
! ATGCTCGTTTGAAATGTGAA+GGG + chr8_4:54159904-54159923 Msa1333890:intron 35.0%
TACACAACGTAAAAGTCCAT+AGG - chr8_4:54159956-54159975 None:intergenic 35.0%
TGCAGCATCTTTCTGTTGTT+GGG - chr8_4:54156889-54156908 None:intergenic 40.0%
AAAGATGCTGCAGAACTTAG+AGG + chr8_4:54156897-54156916 Msa1333890:five_prime_UTR 40.0%
AAGATGCTGCAGAACTTAGA+GGG + chr8_4:54156898-54156917 Msa1333890:five_prime_UTR 40.0%
!! CAACCCCTCCTTTTGTTTAT+AGG + chr8_4:54156983-54157002 Msa1333890:five_prime_UTR 40.0%
CGTTGCCTATAAACAAAAGG+AGG - chr8_4:54156991-54157010 None:intergenic 40.0%
CGAGATGGTCAGAAGAAATA+AGG - chr8_4:54157016-54157035 None:intergenic 40.0%
TAAGTGAGAGTGAGAGACTT+AGG - chr8_4:54157052-54157071 None:intergenic 40.0%
TCACAGGAAAATCGAAAACC+AGG + chr8_4:54157281-54157300 Msa1333890:five_prime_UTR 40.0%
! TCTTTTCCCATCTTGTGGTT+TGG - chr8_4:54157311-54157330 None:intergenic 40.0%
GGAAAGTAACACCTCCAATA+TGG - chr8_4:54157406-54157425 None:intergenic 40.0%
TGATAGGCTTGTGATAGCTA+GGG + chr8_4:54157468-54157487 Msa1333890:three_prime_UTR 40.0%
AATGAAGTGTGTGTGTGTGT+GGG - chr8_4:54157544-54157563 None:intergenic 40.0%
AAATGAAGTGTGTGTGTGTG+TGG - chr8_4:54157545-54157564 None:intergenic 40.0%
TGCAATTTACAAGGGATTGG+CGG + chr8_4:54157622-54157641 Msa1333890:intron 40.0%
!!! CACGTAATCTATTTTGGTGG+TGG - chr8_4:54157736-54157755 None:intergenic 40.0%
! TTCATGCACTCCTTGATAGT+CGG + chr8_4:54158068-54158087 Msa1333890:intron 40.0%
ACTAGGGGGATAAGAAGTAA+GGG + chr8_4:54158562-54158581 Msa1333890:intron 40.0%
GCTATGAAGCTGATATGATC+AGG + chr8_4:54158599-54158618 Msa1333890:intron 40.0%
! TAGATCCTGCATAATGGAAC+GGG - chr8_4:54158720-54158739 None:intergenic 40.0%
! GTAGATCCTGCATAATGGAA+CGG - chr8_4:54158721-54158740 None:intergenic 40.0%
ACTTCTGCTATAAAAGGCGA+AGG + chr8_4:54158821-54158840 Msa1333890:three_prime_UTR 40.0%
! AAAAGGCGAAGGACTTTTTG+AGG + chr8_4:54158832-54158851 Msa1333890:three_prime_UTR 40.0%
! AAAGGCGAAGGACTTTTTGA+GGG + chr8_4:54158833-54158852 Msa1333890:three_prime_UTR 40.0%
TCATCAACTCAGTTAGGACT+TGG + chr8_4:54159001-54159020 Msa1333890:intron 40.0%
CATCAACTCAGTTAGGACTT+GGG + chr8_4:54159002-54159021 Msa1333890:intron 40.0%
TGGCCAGATCAATGTTGAAT+TGG + chr8_4:54159358-54159377 Msa1333890:intron 40.0%
GGCCAGATCAATGTTGAATT+GGG + chr8_4:54159359-54159378 Msa1333890:intron 40.0%
CACCCAATTCAACATTGATC+TGG - chr8_4:54159364-54159383 None:intergenic 40.0%
CAGATCAATGTTGAATTGGG+TGG + chr8_4:54159362-54159381 Msa1333890:intron 40.0%
! ATGTGAAGGGAGGTATATCT+TGG + chr8_4:54159917-54159936 Msa1333890:intron 40.0%
TGGTCTAGGAAATATGCCTA+TGG + chr8_4:54159937-54159956 Msa1333890:three_prime_UTR 40.0%
GTGTGTGTGTGTGAATGTGT+AGG + chr8_4:54156682-54156701 Msa1333890:five_prime_UTR 45.0%
CAGCATCTTTCTGTTGTTGG+GGG - chr8_4:54156887-54156906 None:intergenic 45.0%
GCAGCATCTTTCTGTTGTTG+GGG - chr8_4:54156888-54156907 None:intergenic 45.0%
CTGCAGCATCTTTCTGTTGT+TGG - chr8_4:54156890-54156909 None:intergenic 45.0%
! GAGAGACTTAGGTTAGTTGC+TGG - chr8_4:54157041-54157060 None:intergenic 45.0%
AACTGCGGTAGCTCATATTG+TGG + chr8_4:54157112-54157131 Msa1333890:five_prime_UTR 45.0%
GCATGTCAGAACTACTGCTT+GGG - chr8_4:54157201-54157220 None:intergenic 45.0%
CAGGAAAATCGAAAACCAGG+TGG + chr8_4:54157284-54157303 Msa1333890:five_prime_UTR 45.0%
TTGTCCATAGGCCATATTGG+AGG + chr8_4:54157392-54157411 Msa1333890:CDS 45.0%
AACACCTCCAATATGGCCTA+TGG - chr8_4:54157399-54157418 None:intergenic 45.0%
CTGATAGGCTTGTGATAGCT+AGG + chr8_4:54157467-54157486 Msa1333890:three_prime_UTR 45.0%
CACATACCTCCAGAATTGCA+TGG - chr8_4:54157501-54157520 None:intergenic 45.0%
GTGGGTGTATGTGTTGTATC+AGG - chr8_4:54157526-54157545 None:intergenic 45.0%
! TGAGCTTAGCTCAGTTGGTA+TGG + chr8_4:54157693-54157712 Msa1333890:intron 45.0%
CACTAGGGGGATAAGAAGTA+AGG + chr8_4:54158561-54158580 Msa1333890:intron 45.0%
TCCATTATGCAGGATCTACC+AGG + chr8_4:54158722-54158741 Msa1333890:intron 45.0%
!! GACTTTTTGAGGGACTTGAC+TGG + chr8_4:54158843-54158862 Msa1333890:three_prime_UTR 45.0%
! CTCGTTTGAAATGTGAAGGG+AGG + chr8_4:54159907-54159926 Msa1333890:intron 45.0%
! AGGGAGGTATATCTTGGTCT+AGG + chr8_4:54159923-54159942 Msa1333890:intron 45.0%
AGAGGGTCTGCACCTCTTTA+TGG + chr8_4:54156915-54156934 Msa1333890:five_prime_UTR 50.0%
GGCATGTCAGAACTACTGCT+TGG - chr8_4:54157202-54157221 None:intergenic 50.0%
!! ATCTTGTGGTTTGGTCCACC+TGG - chr8_4:54157302-54157321 None:intergenic 50.0%
AGGTGGACCAAACCACAAGA+TGG + chr8_4:54157301-54157320 Msa1333890:exon 50.0%
GGTGGACCAAACCACAAGAT+GGG + chr8_4:54157302-54157321 Msa1333890:exon 50.0%
TAGGCTTGTGATAGCTAGGG+AGG + chr8_4:54157471-54157490 Msa1333890:three_prime_UTR 50.0%
GGAGGAGTTCCATGCAATTC+TGG + chr8_4:54157489-54157508 Msa1333890:three_prime_UTR 50.0%
GGAGTTCCATGCAATTCTGG+AGG + chr8_4:54157492-54157511 Msa1333890:three_prime_UTR 50.0%
TAGGCGTCTCCTAAGCTAAG+AGG + chr8_4:54157815-54157834 Msa1333890:intron 50.0%
TGTGTCCCGTTCCATTATGC+AGG + chr8_4:54158712-54158731 Msa1333890:intron 50.0%
GCCTGGTAGATCCTGCATAA+TGG - chr8_4:54158726-54158745 None:intergenic 50.0%
CGTCACTGCAGCATCATCAA+GGG - chr8_4:54158750-54158769 None:intergenic 50.0%
!! CCTTGATGATGCTGCAGTGA+CGG + chr8_4:54158748-54158767 Msa1333890:three_prime_UTR 50.0%
GTAGCAGCCACTAGTTGGAT+TGG - chr8_4:54159441-54159460 None:intergenic 50.0%
GACTAGTAGCAGCCACTAGT+TGG - chr8_4:54159446-54159465 None:intergenic 50.0%
GTATGACTGTGCAGCGATCT+TGG + chr8_4:54159780-54159799 Msa1333890:intron 50.0%
! GGTTAGTTGCTGGACCGAGA+TGG - chr8_4:54157031-54157050 None:intergenic 55.0%
CCAACTGAGCTAAGCTCACG+AGG - chr8_4:54157691-54157710 None:intergenic 55.0%
CCTCGTGAGCTTAGCTCAGT+TGG + chr8_4:54157688-54157707 Msa1333890:intron 55.0%
GGGGGATAAGAAGTAAGGGC+TGG + chr8_4:54158566-54158585 Msa1333890:intron 55.0%
CCGTCACTGCAGCATCATCA+AGG - chr8_4:54158751-54158770 None:intergenic 55.0%
TGACTGTGCAGCGATCTTGG+TGG + chr8_4:54159783-54159802 Msa1333890:intron 55.0%
!! GCAGCATCATCAAGGGCGCC+TGG - chr8_4:54158743-54158762 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 54156669 54159976 54156669 ID=Msa1333890;Name=Msa1333890
chr8_4 mRNA 54156669 54159976 54156669 ID=Msa1333890-mRNA-1;Parent=Msa1333890;Name=Msa1333890-mRNA-1;_AED=0.15;_eAED=0.15;_QI=651|0.66|0.75|0.75|1|1|4|302|40
chr8_4 exon 54156669 54157513 54156669 ID=Msa1333890-mRNA-1:exon:16776;Parent=Msa1333890-mRNA-1
chr8_4 exon 54158474 54158521 54158474 ID=Msa1333890-mRNA-1:exon:16777;Parent=Msa1333890-mRNA-1
chr8_4 exon 54158734 54158864 54158734 ID=Msa1333890-mRNA-1:exon:16778;Parent=Msa1333890-mRNA-1
chr8_4 exon 54159925 54159976 54159925 ID=Msa1333890-mRNA-1:exon:16779;Parent=Msa1333890-mRNA-1
chr8_4 five_prime_UTR 54156669 54157319 54156669 ID=Msa1333890-mRNA-1:five_prime_utr;Parent=Msa1333890-mRNA-1
chr8_4 CDS 54157320 54157442 54157320 ID=Msa1333890-mRNA-1:cds;Parent=Msa1333890-mRNA-1
chr8_4 three_prime_UTR 54157443 54157513 54157443 ID=Msa1333890-mRNA-1:three_prime_utr;Parent=Msa1333890-mRNA-1
chr8_4 three_prime_UTR 54158474 54158521 54158474 ID=Msa1333890-mRNA-1:three_prime_utr;Parent=Msa1333890-mRNA-1
chr8_4 three_prime_UTR 54158734 54158864 54158734 ID=Msa1333890-mRNA-1:three_prime_utr;Parent=Msa1333890-mRNA-1
chr8_4 three_prime_UTR 54159925 54159976 54159925 ID=Msa1333890-mRNA-1:three_prime_utr;Parent=Msa1333890-mRNA-1
Gene Sequence

>Msa1333890

ATGGGAAAAGAATTCATCCCTGATATATATGTAGTAAATTTCTATAACCTTTTACTGTCTTTGATTATTTTTTTGTCCATAGGCCATATTGGAGGTGTTACTTTCCAAATACTCAAGCAATAA
Protein sequence

>Msa1333890

MGKEFIPDIYVVNFYNLLLSLIIFLSIGHIGGVTFQILKQ*