AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0230150


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0230150 MtrunA17_Chr3g0137921 100.000 86 0 0 1 86 264 349 3.46e-59 184
Msa0230150 MtrunA17_Chr4g0063601 82.558 86 15 0 1 86 232 317 6.06e-49 157
Msa0230150 MtrunA17_Chr2g0307091 72.222 90 20 3 1 85 260 349 1.60e-37 128
Msa0230150 MtrunA17_Chr2g0312911 49.231 65 28 2 1 64 258 318 1.52e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0230150 AT5G25400.1 81.395 86 16 0 1 86 264 349 5.73e-48 155
Msa0230150 AT4G32390.1 79.310 87 17 1 1 86 264 350 2.32e-45 149
Msa0230150 AT2G25520.1 79.070 86 16 1 1 86 264 347 5.36e-44 145
Msa0230150 AT5G11230.1 82.353 85 14 1 1 84 264 348 1.01e-42 142

Find 19 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGGTATGGTCTTGCTTTCT+TGG 0.200875 2_2:-1996116 Msa0230150:CDS
GTTACACCCATTAATTTGTT+TGG 0.247768 2_2:-1996136 Msa0230150:CDS
ACCATACCCAAACAAATTAA+TGG 0.252828 2_2:+1996129 None:intergenic
TTATAATCATTCAAAGTTGC+AGG 0.345135 2_2:-1996083 Msa0230150:CDS
TTCAAAGTTGCAGGCACTTA+AGG 0.351119 2_2:-1996074 Msa0230150:CDS
TTACACCCATTAATTTGTTT+GGG 0.369732 2_2:-1996135 Msa0230150:CDS
GGGTATGGTCTTGCTTTCTT+GGG 0.380931 2_2:-1996115 Msa0230150:CDS
CCCATTAATTTGTTTGGGTA+TGG 0.385268 2_2:-1996130 Msa0230150:CDS
GCTTTGACAATGAATGTTGC+TGG 0.418945 2_2:-1996214 None:intergenic
ACACAAGCTGATGAAGAAAC+TGG 0.430294 2_2:-1996028 Msa0230150:CDS
TGGTCTTGCTTTCTTGGGTG+TGG 0.436023 2_2:-1996110 Msa0230150:CDS
CCATACCCAAACAAATTAAT+GGG 0.439143 2_2:+1996130 None:intergenic
TTGCTGGTGTTGTTAAAGAT+TGG 0.469874 2_2:-1996198 Msa0230150:CDS
GATAGAGAAGGTGATGGATC+AGG 0.496335 2_2:-1995992 Msa0230150:CDS
GGCTAAAGAAGCACAGAAGA+AGG 0.498350 2_2:-1996053 Msa0230150:CDS
CTTGAGGATAGAGAAGGTGA+TGG 0.583598 2_2:-1995998 Msa0230150:CDS
AGGTTGCTTGAGGATAGAGA+AGG 0.638167 2_2:-1996004 Msa0230150:CDS
AAGCTGATGAAGAAACTGGA+AGG 0.653904 2_2:-1996024 Msa0230150:CDS
AGAAACTGGAAGGTTGCTTG+AGG 0.654156 2_2:-1996014 Msa0230150:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTACACCCATTAATTTGTTT+GGG - chr2_2:1996037-1996056 Msa0230150:CDS 25.0%
! TTATAATCATTCAAAGTTGC+AGG - chr2_2:1996089-1996108 Msa0230150:CDS 25.0%
! TTTTTCATGGAGTGTGATTA+AGG - chr2_2:1996008-1996027 Msa0230150:CDS 30.0%
!! GTTACACCCATTAATTTGTT+TGG - chr2_2:1996036-1996055 Msa0230150:CDS 30.0%
CCATACCCAAACAAATTAAT+GGG + chr2_2:1996045-1996064 None:intergenic 30.0%
ACCATACCCAAACAAATTAA+TGG + chr2_2:1996046-1996065 None:intergenic 30.0%
!! TTGCTGGTGTTGTTAAAGAT+TGG - chr2_2:1995974-1995993 Msa0230150:CDS 35.0%
!!! GGCTTTTGATTGCTTTTTCA+TGG - chr2_2:1995995-1996014 Msa0230150:CDS 35.0%
!! CCCATTAATTTGTTTGGGTA+TGG - chr2_2:1996042-1996061 Msa0230150:CDS 35.0%
TTCAAAGTTGCAGGCACTTA+AGG - chr2_2:1996098-1996117 Msa0230150:CDS 40.0%
ACACAAGCTGATGAAGAAAC+TGG - chr2_2:1996144-1996163 Msa0230150:CDS 40.0%
AAGCTGATGAAGAAACTGGA+AGG - chr2_2:1996148-1996167 Msa0230150:CDS 40.0%
! TGGGTATGGTCTTGCTTTCT+TGG - chr2_2:1996056-1996075 Msa0230150:CDS 45.0%
! GGGTATGGTCTTGCTTTCTT+GGG - chr2_2:1996057-1996076 Msa0230150:CDS 45.0%
! GGCTAAAGAAGCACAGAAGA+AGG - chr2_2:1996119-1996138 Msa0230150:CDS 45.0%
AGAAACTGGAAGGTTGCTTG+AGG - chr2_2:1996158-1996177 Msa0230150:CDS 45.0%
AGGTTGCTTGAGGATAGAGA+AGG - chr2_2:1996168-1996187 Msa0230150:CDS 45.0%
CTTGAGGATAGAGAAGGTGA+TGG - chr2_2:1996174-1996193 Msa0230150:CDS 45.0%
!! GATAGAGAAGGTGATGGATC+AGG - chr2_2:1996180-1996199 Msa0230150:CDS 45.0%
! TGGTCTTGCTTTCTTGGGTG+TGG - chr2_2:1996062-1996081 Msa0230150:CDS 50.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 1995967 1996227 1995967 ID=Msa0230150;Name=Msa0230150
chr2_2 mRNA 1995967 1996227 1995967 ID=Msa0230150-mRNA-1;Parent=Msa0230150;Name=Msa0230150-mRNA-1;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|86
chr2_2 exon 1995967 1996227 1995967 ID=Msa0230150-mRNA-1:exon:798;Parent=Msa0230150-mRNA-1
chr2_2 CDS 1995967 1996227 1995967 ID=Msa0230150-mRNA-1:cds;Parent=Msa0230150-mRNA-1
Gene Sequence

>Msa0230150

ATGAATGTTGCTGGTGTTGTTAAAGATTGGCTTTTGATTGCTTTTTCATGGAGTGTGATTAAGGATACTGTTACACCCATTAATTTGTTTGGGTATGGTCTTGCTTTCTTGGGTGTGGCTTATTATAATCATTCAAAGTTGCAGGCACTTAAGGCTAAAGAAGCACAGAAGAAGGTTACACAAGCTGATGAAGAAACTGGAAGGTTGCTTGAGGATAGAGAAGGTGATGGATCAGGCAAGAGAAATGATCAGCAGAATTGA

Protein sequence

>Msa0230150

MNVAGVVKDWLLIAFSWSVIKDTVTPINLFGYGLAFLGVAYYNHSKLQALKAKEAQKKVTQADEETGRLLEDREGDGSGKRNDQQN*