AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0142980


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0142980 MtrunA17_Chr2g0287331 53.390 118 19 2 1 82 2 119 3.82e-26 94.7
Msa0142980 MtrunA17_Chr2g0286581 33.702 181 67 5 2 130 17 196 1.47e-21 85.1
Msa0142980 MtrunA17_Chr2g0287401 37.654 162 65 4 2 130 7 165 6.87e-19 77.8
Msa0142980 MtrunA17_Chr2g0287391 38.506 174 55 8 2 124 7 179 1.17e-15 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 21 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCTCCTCAAAGTTGATA+TGG 0.279492 1_4:+1905529 Msa0142980:CDS
TGTTTCACTTTGTCTTCATC+TGG 0.292772 1_4:-1905434 None:intergenic
TGATGATCGAAATCCTTGTT+AGG 0.329238 1_4:+1905199 Msa0142980:CDS
TCAACACCAGCATCTTGTTC+TGG 0.347187 1_4:-1905371 None:intergenic
GATGATTGACGTTGCTCATT+TGG 0.379191 1_4:+1905463 Msa0142980:CDS
CAATCCATCCTCCACAGCTT+GGG 0.389170 1_4:-1905555 None:intergenic
ATTTCGATCATCAGTTCTAT+AGG 0.398462 1_4:-1905189 None:intergenic
GGGTTTCCATATCAACTTTG+AGG 0.427266 1_4:-1905535 None:intergenic
TCAATCCATCCTCCACAGCT+TGG 0.467200 1_4:-1905556 None:intergenic
GATGATCGAAATCCTTGTTA+GGG 0.505946 1_4:+1905200 Msa0142980:CDS
CGAGCAATCCTCTGCAAATG+AGG 0.518022 1_4:+1905229 Msa0142980:CDS
GAATTTACTTTCTATCAAAG+AGG 0.536200 1_4:+1905499 Msa0142980:CDS
GAAACCCAAGCTGTGGAGGA+TGG 0.553602 1_4:+1905551 Msa0142980:CDS
TCTATAGGTAGCACCGTGAT+CGG 0.555660 1_4:-1905174 None:intergenic
CAAACGCACCTCATTTGCAG+AGG 0.579197 1_4:-1905237 None:intergenic
CATCATCCAGAACAAGATGC+TGG 0.583270 1_4:+1905365 Msa0142980:CDS
GATGGCGGAGTCGCCGATCA+CGG 0.583873 1_4:+1905161 None:intergenic
ATGAAGACAAAGTGAAACAA+TGG 0.590534 1_4:+1905438 Msa0142980:CDS
TGCTCGAGTCAACCCTAACA+AGG 0.609205 1_4:-1905212 None:intergenic
TGATATGGAAACCCAAGCTG+TGG 0.630518 1_4:+1905544 Msa0142980:CDS
TATGGAAACCCAAGCTGTGG+AGG 0.654505 1_4:+1905547 Msa0142980:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GAATTTACTTTCTATCAAAG+AGG + chr1_4:1905499-1905518 Msa0142980:CDS 25.0%
ATTTCGATCATCAGTTCTAT+AGG - chr1_4:1905192-1905211 None:intergenic 30.0%
! AGTGGATAATTCCACATTTT+AGG - chr1_4:1905291-1905310 None:intergenic 30.0%
ATGAAGACAAAGTGAAACAA+TGG + chr1_4:1905438-1905457 Msa0142980:CDS 30.0%
GATAAATTCGCAAAAAGCTT+CGG - chr1_4:1905589-1905608 None:intergenic 30.0%
TGATGATCGAAATCCTTGTT+AGG + chr1_4:1905199-1905218 Msa0142980:CDS 35.0%
GATGATCGAAATCCTTGTTA+GGG + chr1_4:1905200-1905219 Msa0142980:CDS 35.0%
TGTTTCACTTTGTCTTCATC+TGG - chr1_4:1905437-1905456 None:intergenic 35.0%
!! AACTCTCCTCAAAGTTGATA+TGG + chr1_4:1905529-1905548 Msa0142980:CDS 35.0%
TGGTCGTTGATCCTAAAATG+TGG + chr1_4:1905277-1905296 Msa0142980:intron 40.0%
AGTCGCTGATCTAACTTCTA+AGG + chr1_4:1905313-1905332 Msa0142980:intron 40.0%
GATGATTGACGTTGCTCATT+TGG + chr1_4:1905463-1905482 Msa0142980:CDS 40.0%
GGGTTTCCATATCAACTTTG+AGG - chr1_4:1905538-1905557 None:intergenic 40.0%
TCTATAGGTAGCACCGTGAT+CGG - chr1_4:1905177-1905196 None:intergenic 45.0%
AGTTAGATCAGCGACTTCAG+TGG - chr1_4:1905309-1905328 None:intergenic 45.0%
CATCATCCAGAACAAGATGC+TGG + chr1_4:1905365-1905384 Msa0142980:CDS 45.0%
TCAACACCAGCATCTTGTTC+TGG - chr1_4:1905374-1905393 None:intergenic 45.0%
TGATATGGAAACCCAAGCTG+TGG + chr1_4:1905544-1905563 Msa0142980:CDS 45.0%
TGCTCGAGTCAACCCTAACA+AGG - chr1_4:1905215-1905234 None:intergenic 50.0%
CGAGCAATCCTCTGCAAATG+AGG + chr1_4:1905229-1905248 Msa0142980:CDS 50.0%
CAAACGCACCTCATTTGCAG+AGG - chr1_4:1905240-1905259 None:intergenic 50.0%
!! TTGCAAGCTCTGGAAATCGC+TGG + chr1_4:1905257-1905276 Msa0142980:intron 50.0%
TATGGAAACCCAAGCTGTGG+AGG + chr1_4:1905547-1905566 Msa0142980:CDS 50.0%
CAATCCATCCTCCACAGCTT+GGG - chr1_4:1905558-1905577 None:intergenic 50.0%
TCAATCCATCCTCCACAGCT+TGG - chr1_4:1905559-1905578 None:intergenic 50.0%
TGAGGTGCGTTTGCAAGCTC+TGG + chr1_4:1905247-1905266 Msa0142980:intron 55.0%
GAAACCCAAGCTGTGGAGGA+TGG + chr1_4:1905551-1905570 Msa0142980:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 1905162 1905634 1905162 ID=Msa0142980;Name=Msa0142980
chr1_4 mRNA 1905162 1905634 1905162 ID=Msa0142980-mRNA-1;Parent=Msa0142980;Name=Msa0142980-mRNA-1;_AED=0.47;_eAED=0.47;_QI=0|0|0|1|1|1|2|0|130
chr1_4 exon 1905162 1905254 1905162 ID=Msa0142980-mRNA-1:exon:718;Parent=Msa0142980-mRNA-1
chr1_4 exon 1905335 1905634 1905335 ID=Msa0142980-mRNA-1:exon:719;Parent=Msa0142980-mRNA-1
chr1_4 CDS 1905162 1905254 1905162 ID=Msa0142980-mRNA-1:cds;Parent=Msa0142980-mRNA-1
chr1_4 CDS 1905335 1905634 1905335 ID=Msa0142980-mRNA-1:cds;Parent=Msa0142980-mRNA-1
Gene Sequence

>Msa0142980

ATGGCGGAGTCGCCGATCACGGTGCTACCTATAGAACTGATGATCGAAATCCTTGTTAGGGTTGACTCGAGCAATCCTCTGCAAATGAGGTGCGTCAAGAAACACATTGATGCTTTCAAATCGCATCATCCAGAACAAGATGCTGGTGTTGAAGATGCTGCTGAAGAAAAAGAAGAAAAAGACGATGCTGCTCCAGATGAAGACAAAGTGAAACAATGGTTGATGATTGACGTTGCTCATTTGGATAAGCTTTTGTTGAATTTACTTTCTATCAAAGAGGATATGAAAACTCTCCTCAAAGTTGATATGGAAACCCAAGCTGTGGAGGATGGATTGAAGTGTTACCGAAGCTTTTTGCGAATTTATCACAAGTTCGCAACTGCTGAAAGTTAA

Protein sequence

>Msa0142980

MAESPITVLPIELMIEILVRVDSSNPLQMRCVKKHIDAFKSHHPEQDAGVEDAAEEKEEKDDAAPDEDKVKQWLMIDVAHLDKLLLNLLSIKEDMKTLLKVDMETQAVEDGLKCYRSFLRIYHKFATAES*