AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1461260


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1461260 MtrunA17_Chr2g0322621 96.748 123 3 1 1 122 1 123 6.63e-82 235
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1461260 AT5G54970.1 44.706 85 44 2 13 94 15 99 2.83e-13 62.0

Find 49 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGGTGGTGGTGGTGAGGC+TGG 0.218611 tig0041406:-11483 Msa1461260:intergenic
CGGAGGCGAGCTAGGGTTCC+TGG 0.289851 tig0041406:-11393 Msa1461260:intergenic
ACCATCGGCGGCTGCGGCGG+CGG 0.308022 tig0041406:-11523 Msa1461260:intergenic
GATCTTGGTCTTGAATCATT+TGG 0.367487 tig0041406:+11660 Msa1461260:CDS
GGTTTCCCTTCCTTCTTCTC+CGG 0.381260 tig0041406:+11543 Msa1461260:CDS
GCTGCTGACTCAGCAGATCT+TGG 0.404060 tig0041406:+11645 Msa1461260:CDS
AGGTCTTGTTCTCTGCTTCA+TGG 0.408985 tig0041406:-11337 Msa1461260:intergenic
TGGAAGTGGTTTGGTAAAGT+AGG 0.419142 tig0041406:-11357 Msa1461260:intergenic
GGTGGTGGTGGTGAGGCTGG+TGG 0.422166 tig0041406:-11480 Msa1461260:intergenic
TGATCGGAGGGAGGTGGTGG+TGG 0.422451 tig0041406:-11492 Msa1461260:intergenic
GTTTCCCTTCCTTCTTCTCC+GGG 0.422834 tig0041406:+11544 Msa1461260:CDS
TTGAGTCACGGAGGCGAGCT+AGG 0.435623 tig0041406:-11401 Msa1461260:intergenic
AGGGAAACCATCGGCGGCTG+CGG 0.436360 tig0041406:-11529 Msa1461260:intergenic
GCGGCTGCGGCGGCGGTTTG+AGG 0.436624 tig0041406:-11516 Msa1461260:intergenic
GGAGTTGAGACGGTGAGATC+TGG 0.440317 tig0041406:-11433 Msa1461260:intergenic
TCCTTCTTCTCCGGGATCTA+CGG 0.444986 tig0041406:+11552 Msa1461260:CDS
GAGCTAGGGTTCCTGGTTTG+AGG 0.446447 tig0041406:-11386 Msa1461260:intergenic
GGCTGATCGGAGGGAGGTGG+TGG 0.453951 tig0041406:-11495 Msa1461260:intergenic
ACCGTAGATCCCGGAGAAGA+AGG 0.460453 tig0041406:-11553 Msa1461260:intergenic
ACCGCCGCCGCAGCCGCCGA+TGG 0.468049 tig0041406:+11522 Msa1461260:CDS
TTCCTGGTTTGAGGAATTTG+TGG 0.469740 tig0041406:-11377 Msa1461260:intergenic
AGGAATTTGTGGAAGTGGTT+TGG 0.473781 tig0041406:-11366 Msa1461260:intergenic
GTTTGAGGAATTTGTGGAAG+TGG 0.474784 tig0041406:-11371 Msa1461260:intergenic
TCTGCTGAGTCAGCAGCAGC+AGG 0.484937 tig0041406:-11639 Msa1461260:intergenic
CTTGGTCTTGAATCATTTGG+TGG 0.505567 tig0041406:+11663 Msa1461260:CDS
GAAACCATCGGCGGCTGCGG+CGG 0.505671 tig0041406:-11526 Msa1461260:intergenic
TCAGCAGCAGCAGGACTACC+AGG 0.510522 tig0041406:-11630 Msa1461260:intergenic
TGAGGCTGATCGGAGGGAGG+TGG 0.511381 tig0041406:-11498 Msa1461260:intergenic
GTGGTGGTGAGGCTGGTGGA+CGG 0.514928 tig0041406:-11476 Msa1461260:intergenic
GGAGGGAGGTGGTGGTGGTG+AGG 0.526828 tig0041406:-11487 Msa1461260:intergenic
GCGACGAGTTTCTTCCGTTG+TGG 0.540155 tig0041406:-11585 Msa1461260:intergenic
GCGGTTTGAGGCTGATCGGA+GGG 0.544351 tig0041406:-11504 Msa1461260:intergenic
TGAGTCACGGAGGCGAGCTA+GGG 0.544597 tig0041406:-11400 Msa1461260:intergenic
TAGATCCCGGAGAAGAAGGA+AGG 0.559260 tig0041406:-11549 Msa1461260:intergenic
GGCGGCGGTTTGAGGCTGAT+CGG 0.567721 tig0041406:-11508 Msa1461260:intergenic
GGCGGTTTGAGGCTGATCGG+AGG 0.568671 tig0041406:-11505 Msa1461260:intergenic
TGGCGGTTATCTTTGAGTCA+CGG 0.583959 tig0041406:-11413 Msa1461260:intergenic
TCTGAGAGAGGGAGTTGAGA+CGG 0.588321 tig0041406:-11443 Msa1461260:intergenic
GTTTGAGGCTGATCGGAGGG+AGG 0.605220 tig0041406:-11501 Msa1461260:intergenic
TTCCACAAATTCCTCAAACC+AGG 0.613859 tig0041406:+11375 Msa1461260:CDS
GAAGAAGGAAGGGAAACCAT+CGG 0.615545 tig0041406:-11538 Msa1461260:intergenic
TTCTTCCGTTGTGGACATCG+AGG 0.627074 tig0041406:-11576 Msa1461260:intergenic
AGATCCCGGAGAAGAAGGAA+GGG 0.635957 tig0041406:-11548 Msa1461260:intergenic
GTAGATTGAGATCTGAGAGA+GGG 0.642846 tig0041406:-11454 Msa1461260:intergenic
GGTAGATTGAGATCTGAGAG+AGG 0.644225 tig0041406:-11455 Msa1461260:intergenic
GTTGAGACGGTGAGATCTGG+CGG 0.649621 tig0041406:-11430 Msa1461260:intergenic
ACGGTCCTCGATGTCCACAA+CGG 0.679494 tig0041406:+11571 Msa1461260:CDS
CGGTTATCTTTGAGTCACGG+AGG 0.737996 tig0041406:-11410 Msa1461250:intergenic
GAAGGAAGGGAAACCATCGG+CGG 0.745154 tig0041406:-11535 Msa1461250:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAGTCAGTTTTCTTTGTTCC+TGG + tig0041406:11612-11631 Msa1461260:CDS 35.0%
!! GATCTTGGTCTTGAATCATT+TGG + tig0041406:11660-11679 Msa1461260:CDS 35.0%
!! TGGAAGTGGTTTGGTAAAGT+AGG - tig0041406:11360-11379 Msa1147410:intergenic 40.0%
AGGAATTTGTGGAAGTGGTT+TGG - tig0041406:11369-11388 Msa1147410:intergenic 40.0%
GTTTGAGGAATTTGTGGAAG+TGG - tig0041406:11374-11393 Msa1147410:intergenic 40.0%
TTCCACAAATTCCTCAAACC+AGG + tig0041406:11375-11394 Msa1461260:CDS 40.0%
! TTCCTGGTTTGAGGAATTTG+TGG - tig0041406:11380-11399 Msa1461260:intergenic 40.0%
GTAGATTGAGATCTGAGAGA+GGG - tig0041406:11457-11476 Msa1461260:intergenic 40.0%
!! CTTGGTCTTGAATCATTTGG+TGG + tig0041406:11663-11682 Msa1461260:CDS 40.0%
TGGCGGTTATCTTTGAGTCA+CGG - tig0041406:11416-11435 Msa1461260:intergenic 45.0%
GGTAGATTGAGATCTGAGAG+AGG - tig0041406:11458-11477 Msa1461260:intergenic 45.0%
GAAGAAGGAAGGGAAACCAT+CGG - tig0041406:11541-11560 Msa1461260:intergenic 45.0%
CGGTTATCTTTGAGTCACGG+AGG - tig0041406:11413-11432 Msa1461260:intergenic 50.0%
TCTGAGAGAGGGAGTTGAGA+CGG - tig0041406:11446-11465 Msa1461260:intergenic 50.0%
GGTTTCCCTTCCTTCTTCTC+CGG + tig0041406:11543-11562 Msa1461260:CDS 50.0%
GTTTCCCTTCCTTCTTCTCC+GGG + tig0041406:11544-11563 Msa1461260:CDS 50.0%
AGATCCCGGAGAAGAAGGAA+GGG - tig0041406:11551-11570 Msa1461260:intergenic 50.0%
TAGATCCCGGAGAAGAAGGA+AGG - tig0041406:11552-11571 Msa1461260:intergenic 50.0%
TCCTTCTTCTCCGGGATCTA+CGG + tig0041406:11552-11571 Msa1461260:CDS 50.0%
TTCTTCCGTTGTGGACATCG+AGG - tig0041406:11579-11598 Msa1461260:intergenic 50.0%
! GAGCTAGGGTTCCTGGTTTG+AGG - tig0041406:11389-11408 Msa1461260:intergenic 55.0%
GTTGAGACGGTGAGATCTGG+CGG - tig0041406:11433-11452 Msa1461260:intergenic 55.0%
GGAGTTGAGACGGTGAGATC+TGG - tig0041406:11436-11455 Msa1461260:intergenic 55.0%
GAAGGAAGGGAAACCATCGG+CGG - tig0041406:11538-11557 Msa1461260:intergenic 55.0%
ACCGTAGATCCCGGAGAAGA+AGG - tig0041406:11556-11575 Msa1461260:intergenic 55.0%
ACATCGAGGACCGTAGATCC+CGG - tig0041406:11565-11584 Msa1461260:intergenic 55.0%
ACGGTCCTCGATGTCCACAA+CGG + tig0041406:11571-11590 Msa1461260:CDS 55.0%
GCGACGAGTTTCTTCCGTTG+TGG - tig0041406:11588-11607 Msa1461260:intergenic 55.0%
!! GCTGCTGACTCAGCAGATCT+TGG + tig0041406:11645-11664 Msa1461260:CDS 55.0%
TGAGTCACGGAGGCGAGCTA+GGG - tig0041406:11403-11422 Msa1461260:intergenic 60.0%
TTGAGTCACGGAGGCGAGCT+AGG - tig0041406:11404-11423 Msa1461260:intergenic 60.0%
! GTTTGAGGCTGATCGGAGGG+AGG - tig0041406:11504-11523 Msa1461260:intergenic 60.0%
! GCGGTTTGAGGCTGATCGGA+GGG - tig0041406:11507-11526 Msa1461260:intergenic 60.0%
TCAGCAGCAGCAGGACTACC+AGG - tig0041406:11633-11652 Msa1461260:intergenic 60.0%
TCTGCTGAGTCAGCAGCAGC+AGG - tig0041406:11642-11661 Msa1461260:intergenic 60.0%
! GTGGTGGTGAGGCTGGTGGA+CGG - tig0041406:11479-11498 Msa1461260:intergenic 65.0%
! TGATCGGAGGGAGGTGGTGG+TGG - tig0041406:11495-11514 Msa1461260:intergenic 65.0%
TGAGGCTGATCGGAGGGAGG+TGG - tig0041406:11501-11520 Msa1461260:intergenic 65.0%
! GGCGGTTTGAGGCTGATCGG+AGG - tig0041406:11508-11527 Msa1461260:intergenic 65.0%
! GGCGGCGGTTTGAGGCTGAT+CGG - tig0041406:11511-11530 Msa1461260:intergenic 65.0%
AGGGAAACCATCGGCGGCTG+CGG - tig0041406:11532-11551 Msa1461260:intergenic 65.0%
CGGAGGCGAGCTAGGGTTCC+TGG - tig0041406:11396-11415 Msa1461260:intergenic 70.0%
!! GGTGGTGGTGGTGAGGCTGG+TGG - tig0041406:11483-11502 Msa1461260:intergenic 70.0%
!! GGAGGTGGTGGTGGTGAGGC+TGG - tig0041406:11486-11505 Msa1461260:intergenic 70.0%
!! GGAGGGAGGTGGTGGTGGTG+AGG - tig0041406:11490-11509 Msa1461260:intergenic 70.0%
GGCTGATCGGAGGGAGGTGG+TGG - tig0041406:11498-11517 Msa1461260:intergenic 70.0%
GAAACCATCGGCGGCTGCGG+CGG - tig0041406:11529-11548 Msa1461260:intergenic 70.0%
! GCGGCTGCGGCGGCGGTTTG+AGG - tig0041406:11519-11538 Msa1461260:intergenic 80.0%
! ACCATCGGCGGCTGCGGCGG+CGG - tig0041406:11526-11545 Msa1461260:intergenic 80.0%
! ACCGCCGCCGCAGCCGCCGA+TGG + tig0041406:11522-11541 Msa1461260:CDS 85.0%
Chromosome Type Strat End Strand Name
tig0041406 gene 11339 11707 11339 ID=Msa1461260;Name=Msa1461260
tig0041406 mRNA 11339 11707 11339 ID=Msa1461260-mRNA-1;Parent=Msa1461260;Name=Msa1461260-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|122
tig0041406 exon 11339 11707 11339 ID=Msa1461260-mRNA-1:exon:23094;Parent=Msa1461260-mRNA-1
tig0041406 CDS 11339 11707 11339 ID=Msa1461260-mRNA-1:cds;Parent=Msa1461260-mRNA-1
Gene Sequence

>Msa1461260

ATGAAGCAGAGAACAAGACCTACTTTACCAAACCACTTCCACAAATTCCTCAAACCAGGAACCCTAGCTCGCCTCCGTGACTCAAAGATAACCGCCAGATCTCACCGTCTCAACTCCCTCTCTCAGATCTCAATCTACCGTCCACCAGCCTCACCACCACCACCTCCCTCCGATCAGCCTCAAACCGCCGCCGCAGCCGCCGATGGTTTCCCTTCCTTCTTCTCCGGGATCTACGGTCCTCGATGTCCACAACGGAAGAAACTCGTCGCTGCAAAGTCAGTTTTCTTTGTTCCTGGTAGTCCTGCTGCTGCTGACTCAGCAGATCTTGGTCTTGAATCATTTGGTGGCGATATTATCGCTGCTAATTGA

Protein sequence

>Msa1461260

MKQRTRPTLPNHFHKFLKPGTLARLRDSKITARSHRLNSLSQISIYRPPASPPPPPSDQPQTAAAAADGFPSFFSGIYGPRCPQRKKLVAAKSVFFVPGSPAAADSADLGLESFGGDIIAAN*