AlfalfaGEDB | MS.gene004124

M. sativa cultivar XinJiangDaYe / MS.gene004124

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene004124.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene004124.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene004124.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene64920	MS.gene004124	PPI
MS.gene060463	MS.gene004124	PPI
MS.gene60093	MS.gene004124	PPI
MS.gene018578	MS.gene004124	PPI
MS.gene31196	MS.gene004124	PPI
MS.gene89488	MS.gene004124	PPI
MS.gene010219	MS.gene004124	PPI
MS.gene060465	MS.gene004124	PPI
MS.gene04068	MS.gene004124	PPI
MS.gene061867	MS.gene004124	PPI
MS.gene004124	MS.gene64650	PPI
MS.gene004124	MS.gene60093	PPI
MS.gene08121	MS.gene004124	PPI
MS.gene022932	MS.gene004124	PPI
MS.gene004124	MS.gene04972	PPI
MS.gene004124	MS.gene004121	PPI
MS.gene88681	MS.gene004124	PPI
MS.gene035718	MS.gene004124	PPI
MS.gene035896	MS.gene004124	PPI
MS.gene004124	MS.gene018578	PPI
MS.gene73136	MS.gene004124	PPI
MS.gene008276	MS.gene004124	PPI
MS.gene070907	MS.gene004124	PPI
MS.gene004124	MS.gene018580	PPI
MS.gene009027	MS.gene004124	PPI
MS.gene09873	MS.gene004124	PPI
MS.gene78578	MS.gene004124	PPI
MS.gene04052	MS.gene004124	PPI
MS.gene08122	MS.gene004124	PPI
MS.gene048705	MS.gene004124	PPI
MS.gene05623	MS.gene004124	PPI
MS.gene004124	MS.gene89488	PPI
MS.gene043967	MS.gene004124	PPI
MS.gene84536	MS.gene004124	PPI
MS.gene04972	MS.gene004124	PPI
MS.gene028629	MS.gene004124	PPI
MS.gene82546	MS.gene004124	PPI
MS.gene004124	MS.gene061874	PPI
MS.gene04433	MS.gene004124	PPI
MS.gene04067	MS.gene004124	PPI
MS.gene71215	MS.gene004124	PPI
MS.gene061874	MS.gene004124	PPI
MS.gene061866	MS.gene004124	PPI
MS.gene061870	MS.gene004124	PPI
MS.gene004121	MS.gene004124	PPI
MS.gene66225	MS.gene004124	PPI
MS.gene70955	MS.gene004124	PPI
MS.gene071136	MS.gene004124	PPI
MS.gene040383	MS.gene004124	PPI
MS.gene004124	MS.gene016624	PPI
MS.gene070242	MS.gene004124	PPI
MS.gene012390	MS.gene004124	PPI
MS.gene79195	MS.gene004124	PPI
MS.gene035721	MS.gene004124	PPI
MS.gene056215	MS.gene004124	PPI
MS.gene061869	MS.gene004124	PPI
MS.gene064557	MS.gene004124	PPI
MS.gene038469	MS.gene004124	PPI
MS.gene61017	MS.gene004124	PPI
MS.gene04452	MS.gene004124	PPI
MS.gene64650	MS.gene004124	PPI
MS.gene035787	MS.gene004124	PPI
MS.gene004124	MS.gene049705	PPI
MS.gene04576	MS.gene004124	PPI
MS.gene89487	MS.gene004124	PPI
MS.gene035719	MS.gene004124	PPI
MS.gene58854	MS.gene004124	PPI
MS.gene016624	MS.gene004124	PPI
MS.gene004124	MS.gene68545	PPI
MS.gene04392	MS.gene004124	PPI
MS.gene072493	MS.gene004124	PPI
MS.gene035720	MS.gene004124	PPI
MS.gene30920	MS.gene004124	PPI
MS.gene048746	MS.gene004124	PPI
MS.gene010220	MS.gene004124	PPI
MS.gene08053	MS.gene004124	PPI
MS.gene061872	MS.gene004124	PPI
MS.gene88680	MS.gene004124	PPI
MS.gene68545	MS.gene004124	PPI
MS.gene070241	MS.gene004124	PPI
MS.gene04434	MS.gene004124	PPI
MS.gene049821	MS.gene004124	PPI
MS.gene022946	MS.gene004124	PPI
MS.gene62520	MS.gene004124	PPI
MS.gene018580	MS.gene004124	PPI
MS.gene04064	MS.gene004124	PPI
MS.gene061876	MS.gene004124	PPI
MS.gene05619	MS.gene004124	PPI
MS.gene05608	MS.gene004124	PPI
MS.gene012391	MS.gene004124	PPI
MS.gene049705	MS.gene004124	PPI
MS.gene070244	MS.gene004124	PPI
MS.gene09871	MS.gene004124	PPI
MS.gene035897	MS.gene004124	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene004124.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene004124.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene004124.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene004124.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene004124.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene004124.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene004124.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene004124.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene004124.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene004124.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene004124.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene004124.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene004124.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene004124.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene004124.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene004124.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene004124.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene004124.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene004124.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene004124.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene004124.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene004124.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene004124.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene004124.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene004124.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene004124.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene004124.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 38 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGAACCCTCTACGGATTT+GGG	0.267091	4.2:-42651598	MS.gene004124:CDS
GGAAGAACCCTCTACGGATT+TGG	0.284984	4.2:-42651599	MS.gene004124:CDS
TGATTTCTGATCAAGATGTC+TGG	0.330252	4.2:-42651896	None:intergenic
TGGCGTGTTGAAGATCTTCT+TGG	0.331421	4.2:-42651714	MS.gene004124:CDS
CTTCTTGGAGAATGTGATTA+GGG	0.347463	4.2:-42651699	MS.gene004124:CDS
GGAAAGGGTTTGGGAAAGGG+AGG	0.361300	4.2:-42651860	MS.gene004124:CDS
TCTTCTTGGAGAATGTGATT+AGG	0.365922	4.2:-42651700	MS.gene004124:CDS
GGAAAAGGAGGAAAGGGTTT+GGG	0.378357	4.2:-42651869	MS.gene004124:CDS
TATCAGTGGTTTGATTTATG+AGG	0.387367	4.2:-42651744	MS.gene004124:CDS
TGGAAAAGGAGGAAAGGGTT+TGG	0.387838	4.2:-42651870	MS.gene004124:CDS
GGAGGAAAGGGTTTGGGAAA+GGG	0.411122	4.2:-42651863	MS.gene004124:CDS
AAGATGTCTGGGCGTGGAAA+AGG	0.429435	4.2:-42651884	MS.gene004124:CDS
AGGAGGAAAGGGTTTGGGAA+AGG	0.444877	4.2:-42651864	MS.gene004124:CDS
CTTACACTGAGCACGCTAGA+AGG	0.481731	4.2:-42651667	MS.gene004124:CDS
GACTGTTACCGCCATGGATG+TGG	0.483826	4.2:-42651642	MS.gene004124:CDS
TCGCAGGCTTTGTGATTCCC+TGG	0.493877	4.2:+42651801	None:intergenic
GGTTTATGCTCTCAAGAGAC+AGG	0.499176	4.2:-42651621	MS.gene004124:CDS
GGTGCTTCGTGACAACATCC+AGG	0.512203	4.2:-42651819	MS.gene004124:CDS
GATTTCTGATCAAGATGTCT+GGG	0.513702	4.2:-42651895	None:intergenic
TGTTGTCACGAAGCACCTTA+CGG	0.540622	4.2:+42651825	None:intergenic
CGTCGTCTTGCTCGTAGAGG+AGG	0.557881	4.2:-42651776	MS.gene004124:CDS
AAGAACCCTCTACGGATTTG+GGG	0.565022	4.2:-42651597	MS.gene004124:CDS
CTGATCAAGATGTCTGGGCG+TGG	0.573727	4.2:-42651890	None:intergenic
AGGAGCAAAACGACACCGTA+AGG	0.575994	4.2:-42651840	MS.gene004124:CDS
CGAGCAAGACGACGAATCGC+AGG	0.578319	4.2:+42651785	None:intergenic
GGGCGTGGAAAAGGAGGAAA+GGG	0.587932	4.2:-42651875	MS.gene004124:CDS
AAGGAAGACTGTTACCGCCA+TGG	0.588179	4.2:-42651648	MS.gene004124:CDS
GGAGGAGTGAAGCGTATCAG+TGG	0.591400	4.2:-42651758	MS.gene004124:CDS
ATGTCTGGGCGTGGAAAAGG+AGG	0.593086	4.2:-42651881	MS.gene004124:CDS
AGACAGGGAAGAACCCTCTA+CGG	0.600202	4.2:-42651605	MS.gene004124:CDS
TTGATTTATGAGGAGACTCG+TGG	0.604489	4.2:-42651734	MS.gene004124:CDS
TGGGCGTGGAAAAGGAGGAA+AGG	0.624585	4.2:-42651876	MS.gene004124:CDS
GAGAGCATAAACCACATCCA+TGG	0.625099	4.2:+42651631	None:intergenic
AGAACCCTCTACGGATTTGG+GGG	0.641482	4.2:-42651596	MS.gene004124:CDS
GTGCTTCGTGACAACATCCA+GGG	0.652644	4.2:-42651818	MS.gene004124:CDS
ATTCGTCGTCTTGCTCGTAG+AGG	0.654201	4.2:-42651779	MS.gene004124:CDS
GTTTATGCTCTCAAGAGACA+GGG	0.674567	4.2:-42651620	MS.gene004124:CDS
AGCATAAACCACATCCATGG+CGG	0.701499	4.2:+42651634	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TATCAGTGGTTTGATTTATG+AGG	-	chr4.2:42651729-42651748	MS.gene004124:CDS	30.0%
	CTTCTTGGAGAATGTGATTA+GGG	-	chr4.2:42651774-42651793	MS.gene004124:CDS	35.0%
	TCTTCTTGGAGAATGTGATT+AGG	-	chr4.2:42651773-42651792	MS.gene004124:CDS	35.0%
	GTTTATGCTCTCAAGAGACA+GGG	-	chr4.2:42651853-42651872	MS.gene004124:CDS	40.0%
	TTGATTTATGAGGAGACTCG+TGG	-	chr4.2:42651739-42651758	MS.gene004124:CDS	40.0%
	AAGAACCCTCTACGGATTTG+GGG	-	chr4.2:42651876-42651895	MS.gene004124:CDS	45.0%
	AGCATAAACCACATCCATGG+CGG	+	chr4.2:42651842-42651861	None:intergenic	45.0%
	GAAGAACCCTCTACGGATTT+GGG	-	chr4.2:42651875-42651894	MS.gene004124:CDS	45.0%
	GGAAAAGGAGGAAAGGGTTT+GGG	-	chr4.2:42651604-42651623	MS.gene004124:CDS	45.0%
	GGTTTATGCTCTCAAGAGAC+AGG	-	chr4.2:42651852-42651871	MS.gene004124:CDS	45.0%
	TGGAAAAGGAGGAAAGGGTT+TGG	-	chr4.2:42651603-42651622	MS.gene004124:CDS	45.0%
	TGGCGTGTTGAAGATCTTCT+TGG	-	chr4.2:42651759-42651778	MS.gene004124:CDS	45.0%
!	GAGAGCATAAACCACATCCA+TGG	+	chr4.2:42651845-42651864	None:intergenic	45.0%
!	TGTTGTCACGAAGCACCTTA+CGG	+	chr4.2:42651651-42651670	None:intergenic	45.0%
	AAGGAAGACTGTTACCGCCA+TGG	-	chr4.2:42651825-42651844	MS.gene004124:CDS	50.0%
	AGAACCCTCTACGGATTTGG+GGG	-	chr4.2:42651877-42651896	MS.gene004124:CDS	50.0%
	AGACAGGGAAGAACCCTCTA+CGG	-	chr4.2:42651868-42651887	MS.gene004124:CDS	50.0%
	AGGAGCAAAACGACACCGTA+AGG	-	chr4.2:42651633-42651652	MS.gene004124:CDS	50.0%
	AGGAGGAAAGGGTTTGGGAA+AGG	-	chr4.2:42651609-42651628	MS.gene004124:CDS	50.0%
	ATTCGTCGTCTTGCTCGTAG+AGG	-	chr4.2:42651694-42651713	MS.gene004124:CDS	50.0%
	CTAACCCCCAAATCCGTAGA+GGG	+	chr4.2:42651884-42651903	None:intergenic	50.0%
	CTTACACTGAGCACGCTAGA+AGG	-	chr4.2:42651806-42651825	MS.gene004124:CDS	50.0%
	GGAAGAACCCTCTACGGATT+TGG	-	chr4.2:42651874-42651893	MS.gene004124:CDS	50.0%
	GGAGGAAAGGGTTTGGGAAA+GGG	-	chr4.2:42651610-42651629	MS.gene004124:CDS	50.0%
	GTGCTTCGTGACAACATCCA+GGG	-	chr4.2:42651655-42651674	MS.gene004124:CDS	50.0%
	ATGTCTGGGCGTGGAAAAGG+AGG	-	chr4.2:42651592-42651611	MS.gene004124:CDS	55.0%
	GACTGTTACCGCCATGGATG+TGG	-	chr4.2:42651831-42651850	MS.gene004124:CDS	55.0%
	GGAAAGGGTTTGGGAAAGGG+AGG	-	chr4.2:42651613-42651632	MS.gene004124:CDS	55.0%
	GGAGGAGTGAAGCGTATCAG+TGG	-	chr4.2:42651715-42651734	MS.gene004124:CDS	55.0%
	GGGCGTGGAAAAGGAGGAAA+GGG	-	chr4.2:42651598-42651617	MS.gene004124:CDS	55.0%
	GGTGCTTCGTGACAACATCC+AGG	-	chr4.2:42651654-42651673	MS.gene004124:CDS	55.0%
	TGGGCGTGGAAAAGGAGGAA+AGG	-	chr4.2:42651597-42651616	MS.gene004124:CDS	55.0%
!	TCGCAGGCTTTGTGATTCCC+TGG	+	chr4.2:42651675-42651694	None:intergenic	55.0%
	CGAGCAAGACGACGAATCGC+AGG	+	chr4.2:42651691-42651710	None:intergenic	60.0%
	CGTCGTCTTGCTCGTAGAGG+AGG	-	chr4.2:42651697-42651716	MS.gene004124:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	42651592	42651903	42651592	ID=MS.gene004124
chr4.2	mRNA	42651592	42651903	42651592	ID=MS.gene004124.t1;Parent=MS.gene004124
chr4.2	exon	42651592	42651903	42651592	ID=MS.gene004124.t1.exon1;Parent=MS.gene004124.t1
chr4.2	CDS	42651592	42651903	42651592	ID=cds.MS.gene004124.t1;Parent=MS.gene004124.t1

Gene Sequence	>MS.gene004124 ATGTCTGGGCGTGGAAAAGGAGGAAAGGGTTTGGGAAAGGGAGGAGCAAAACGACACCGTAAGGTGCTTCGTGACAACATCCAGGGAATCACAAAGCCTGCGATTCGTCGTCTTGCTCGTAGAGGAGGAGTGAAGCGTATCAGTGGTTTGATTTATGAGGAGACTCGTGGCGTGTTGAAGATCTTCTTGGAGAATGTGATTAGGGATGCTGTGACTTACACTGAGCACGCTAGAAGGAAGACTGTTACCGCCATGGATGTGGTTTATGCTCTCAAGAGACAGGGAAGAACCCTCTACGGATTTGGGGGTTAG
Protein sequence	>MS.gene004124.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG