AlfalfaGEDB | MS.gene09871

M. sativa cultivar XinJiangDaYe / MS.gene09871

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09871.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09871.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09871.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051263	MS.gene09871	0.809354	1.97E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene09871	MS.gene05619	PPI
MS.gene056215	MS.gene09871	PPI
MS.gene064557	MS.gene09871	PPI
MS.gene038469	MS.gene09871	PPI
MS.gene09871	MS.gene04052	PPI
MS.gene09871	MS.gene89488	PPI
MS.gene04433	MS.gene09871	PPI
MS.gene04452	MS.gene09871	PPI
MS.gene09871	MS.gene79195	PPI
MS.gene09871	MS.gene048705	PPI
MS.gene09871	MS.gene61017	PPI
MS.gene09871	MS.gene061874	PPI
MS.gene09871	MS.gene04576	PPI
MS.gene035787	MS.gene09871	PPI
MS.gene09871	MS.gene012390	PPI
MS.gene89487	MS.gene09871	PPI
MS.gene035719	MS.gene09871	PPI
MS.gene09871	MS.gene04434	PPI
MS.gene58854	MS.gene09871	PPI
MS.gene70955	MS.gene09871	PPI
MS.gene040383	MS.gene09871	PPI
MS.gene070242	MS.gene09871	PPI
MS.gene04392	MS.gene09871	PPI
MS.gene072493	MS.gene09871	PPI
MS.gene30920	MS.gene09871	PPI
MS.gene048746	MS.gene09871	PPI
MS.gene010220	MS.gene09871	PPI
MS.gene08053	MS.gene09871	PPI
MS.gene061872	MS.gene09871	PPI
MS.gene09871	MS.gene016624	PPI
MS.gene09871	MS.gene008276	PPI
MS.gene88680	MS.gene09871	PPI
MS.gene09871	MS.gene73136	PPI
MS.gene070241	MS.gene09871	PPI
MS.gene09871	MS.gene049821	PPI
MS.gene022946	MS.gene09871	PPI
MS.gene09871	MS.gene04068	PPI
MS.gene09871	MS.gene05623	PPI
MS.gene061876	MS.gene09871	PPI
MS.gene09871	MS.gene035720	PPI
MS.gene012391	MS.gene09871	PPI
MS.gene070244	MS.gene09871	PPI
MS.gene035897	MS.gene09871	PPI
MS.gene64920	MS.gene09871	PPI
MS.gene060463	MS.gene09871	PPI
MS.gene09871	MS.gene049705	PPI
MS.gene09871	MS.gene070907	PPI
MS.gene09871	MS.gene04392	PPI
MS.gene31196	MS.gene09871	PPI
MS.gene09871	MS.gene010219	PPI
MS.gene09871	MS.gene05608	PPI
MS.gene09871	MS.gene68545	PPI
MS.gene04068	MS.gene09871	PPI
MS.gene09871	MS.gene82546	PPI
MS.gene04434	MS.gene09871	PPI
MS.gene09871	MS.gene048746	PPI
MS.gene08121	MS.gene09871	PPI
MS.gene09871	MS.gene09873	PPI
MS.gene022932	MS.gene09871	PPI
MS.gene88681	MS.gene09871	PPI
MS.gene035718	MS.gene09871	PPI
MS.gene035896	MS.gene09871	PPI
MS.gene09871	MS.gene62520	PPI
MS.gene09871	MS.gene035897	PPI
MS.gene009027	MS.gene09871	PPI
MS.gene78578	MS.gene09871	PPI
MS.gene060465	MS.gene09871	PPI
MS.gene05623	MS.gene09871	PPI
MS.gene043967	MS.gene09871	PPI
MS.gene09871	MS.gene84536	PPI
MS.gene028629	MS.gene09871	PPI
MS.gene09871	MS.gene061866	PPI
MS.gene09871	MS.gene64650	PPI
MS.gene09871	MS.gene60093	PPI
MS.gene09871	MS.gene08122	PPI
MS.gene04067	MS.gene09871	PPI
MS.gene71215	MS.gene09871	PPI
MS.gene061870	MS.gene09871	PPI
MS.gene09871	MS.gene061869	PPI
MS.gene09871	MS.gene04972	PPI
MS.gene09871	MS.gene004121	PPI
MS.gene66225	MS.gene09871	PPI
MS.gene071136	MS.gene09871	PPI
MS.gene09871	MS.gene018578	PPI
MS.gene09871	MS.gene061867	PPI
MS.gene09871	MS.gene04064	PPI
MS.gene09871	MS.gene018580	PPI
MS.gene09871	MS.gene004124	PPI
MS.gene79195	MS.gene09871	PPI
MS.gene035721	MS.gene09871	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09871.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene09871.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene09871.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene09871.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene09871.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene09871.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene09871.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene09871.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene09871.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene09871.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene09871.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene09871.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene09871.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene09871.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene09871.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene09871.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09871.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene09871.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene09871.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene09871.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene09871.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene09871.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene09871.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene09871.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene09871.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene09871.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene09871.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 31 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGTGTTCTCAAGATCTTCT+TGG	0.324000	7.3:+12904270	MS.gene09871:CDS
GGAAAGGGATTGGGAAAGGG+AGG	0.412951	7.3:+12904124	MS.gene09871:CDS
TCGCAGGCTTTGTTATGCCT+TGG	0.436279	7.3:-12904183	None:intergenic
AAAATGTCTGGCCGCGGAAA+AGG	0.446222	7.3:+12904100	None:intergenic
TGTTTATGCTTTGAAGAGAC+AGG	0.447929	7.3:+12904363	MS.gene09871:CDS
GGAGGAAAGGGATTGGGAAA+GGG	0.462159	7.3:+12904121	MS.gene09871:CDS
GGAAGGACTCTCTACGGTTT+CGG	0.462635	7.3:+12904385	MS.gene09871:CDS
AGGAGGAAAGGGATTGGGAA+AGG	0.466050	7.3:+12904120	MS.gene09871:CDS
TGGGAAAGGGAGGAGCAAAA+AGG	0.473619	7.3:+12904134	MS.gene09871:CDS
GGAAAAGGAGGAAAGGGATT+GGG	0.479340	7.3:+12904115	MS.gene09871:CDS
CGGAAAAGGAGGAAAGGGAT+TGG	0.482086	7.3:+12904114	MS.gene09871:CDS
CTGCGATTCGTCGTCTTGCA+AGG	0.502831	7.3:+12904200	MS.gene09871:CDS
GGCCGCGGAAAAGGAGGAAA+GGG	0.550993	7.3:+12904109	MS.gene09871:CDS
CTTGCAAGACGACGAATCGC+AGG	0.552192	7.3:-12904199	None:intergenic
TTACTTACACTGAGCACGCT+AGG	0.572434	7.3:+12904314	MS.gene09871:CDS
ATGTCTGGCCGCGGAAAAGG+AGG	0.578066	7.3:+12904103	MS.gene09871:CDS
GAGGAAGACTGTTACCGCCA+TGG	0.588179	7.3:+12904336	MS.gene09871:CDS
TGGCCGCGGAAAAGGAGGAA+AGG	0.594714	7.3:+12904108	MS.gene09871:CDS
AGACAGGGAAGGACTCTCTA+CGG	0.604561	7.3:+12904379	MS.gene09871:CDS
AGGACTCTCTACGGTTTCGG+TGG	0.607233	7.3:+12904388	MS.gene09871:CDS
CAAAGCATAAACAACATCCA+TGG	0.612707	7.3:-12904353	None:intergenic
CGTCGTCTTGCAAGGCGTGG+TGG	0.615268	7.3:+12904208	MS.gene09871:CDS
GTTTATGCTTTGAAGAGACA+GGG	0.618996	7.3:+12904364	MS.gene09871:CDS
ATGCTTTGAAGAGACAGGGA+AGG	0.619457	7.3:+12904368	MS.gene09871:CDS
ATTCGTCGTCTTGCAAGGCG+TGG	0.619919	7.3:+12904205	MS.gene09871:CDS
GTTCTTCGCGATAACATCCA+AGG	0.633409	7.3:+12904166	MS.gene09871:CDS
AGCGTGCTCAGTGTAAGTAA+CGG	0.650649	7.3:-12904311	None:intergenic
CTTACACTGAGCACGCTAGG+AGG	0.678435	7.3:+12904317	MS.gene09871:CDS
GGTGGTGTGAAGAGAATCAG+TGG	0.694412	7.3:+12904226	MS.gene09871:CDS
CTCATCTATGAAGAGACTCG+CGG	0.697662	7.3:+12904250	MS.gene09871:CDS
AGCATAAACAACATCCATGG+CGG	0.725411	7.3:-12904350	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CAAAGCATAAACAACATCCA+TGG	-	chr7.3:12904356-12904375	None:intergenic	35.0%
!!	GTTTATGCTTTGAAGAGACA+GGG	+	chr7.3:12904364-12904383	MS.gene09871:CDS	35.0%
!!	TGTTTATGCTTTGAAGAGAC+AGG	+	chr7.3:12904363-12904382	MS.gene09871:CDS	35.0%
	AGCATAAACAACATCCATGG+CGG	-	chr7.3:12904353-12904372	None:intergenic	40.0%
	AGCGTGCTCAGTGTAAGTAA+CGG	-	chr7.3:12904314-12904333	None:intergenic	45.0%
	CGGTGTTCTCAAGATCTTCT+TGG	+	chr7.3:12904270-12904289	MS.gene09871:CDS	45.0%
	CTCATCTATGAAGAGACTCG+CGG	+	chr7.3:12904250-12904269	MS.gene09871:CDS	45.0%
	GGAAAAGGAGGAAAGGGATT+GGG	+	chr7.3:12904115-12904134	MS.gene09871:CDS	45.0%
	GTTCTTCGCGATAACATCCA+AGG	+	chr7.3:12904166-12904185	MS.gene09871:CDS	45.0%
	TTACTTACACTGAGCACGCT+AGG	+	chr7.3:12904314-12904333	MS.gene09871:CDS	45.0%
!	AGGAGCAAAAAGGCACAGAA+AGG	+	chr7.3:12904144-12904163	MS.gene09871:CDS	45.0%
!!	ATGCTTTGAAGAGACAGGGA+AGG	+	chr7.3:12904368-12904387	MS.gene09871:CDS	45.0%
	AGACAGGGAAGGACTCTCTA+CGG	+	chr7.3:12904379-12904398	MS.gene09871:CDS	50.0%
	AGGAGGAAAGGGATTGGGAA+AGG	+	chr7.3:12904120-12904139	MS.gene09871:CDS	50.0%
	CGGAAAAGGAGGAAAGGGAT+TGG	+	chr7.3:12904114-12904133	MS.gene09871:CDS	50.0%
	GGAAGGACTCTCTACGGTTT+CGG	+	chr7.3:12904385-12904404	MS.gene09871:CDS	50.0%
	GGAGGAAAGGGATTGGGAAA+GGG	+	chr7.3:12904121-12904140	MS.gene09871:CDS	50.0%
	GGTGGTGTGAAGAGAATCAG+TGG	+	chr7.3:12904226-12904245	MS.gene09871:CDS	50.0%
	TGGGAAAGGGAGGAGCAAAA+AGG	+	chr7.3:12904134-12904153	MS.gene09871:CDS	50.0%
!	ATCCCTTTCCTCCTTTTCCG+CGG	-	chr7.3:12904114-12904133	None:intergenic	50.0%
!!	TCGCAGGCTTTGTTATGCCT+TGG	-	chr7.3:12904186-12904205	None:intergenic	50.0%
	AGGACTCTCTACGGTTTCGG+TGG	+	chr7.3:12904388-12904407	MS.gene09871:CDS	55.0%
	ATTCGTCGTCTTGCAAGGCG+TGG	+	chr7.3:12904205-12904224	MS.gene09871:CDS	55.0%
	CTGCGATTCGTCGTCTTGCA+AGG	+	chr7.3:12904200-12904219	MS.gene09871:CDS	55.0%
	CTTACACTGAGCACGCTAGG+AGG	+	chr7.3:12904317-12904336	MS.gene09871:CDS	55.0%
	CTTGCAAGACGACGAATCGC+AGG	-	chr7.3:12904202-12904221	None:intergenic	55.0%
	GAGGAAGACTGTTACCGCCA+TGG	+	chr7.3:12904336-12904355	MS.gene09871:CDS	55.0%
	GGAAAGGGATTGGGAAAGGG+AGG	+	chr7.3:12904124-12904143	MS.gene09871:CDS	55.0%
	ATGTCTGGCCGCGGAAAAGG+AGG	+	chr7.3:12904103-12904122	MS.gene09871:CDS	60.0%
	GGCCGCGGAAAAGGAGGAAA+GGG	+	chr7.3:12904109-12904128	MS.gene09871:CDS	60.0%
	TGGCCGCGGAAAAGGAGGAA+AGG	+	chr7.3:12904108-12904127	MS.gene09871:CDS	60.0%
	CGTCGTCTTGCAAGGCGTGG+TGG	+	chr7.3:12904208-12904227	MS.gene09871:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	12904103	12904414	12904103	ID=MS.gene09871
chr7.3	mRNA	12904103	12904414	12904103	ID=MS.gene09871.t1;Parent=MS.gene09871
chr7.3	exon	12904103	12904414	12904103	ID=MS.gene09871.t1.exon1;Parent=MS.gene09871.t1
chr7.3	CDS	12904103	12904414	12904103	ID=cds.MS.gene09871.t1;Parent=MS.gene09871.t1

Gene Sequence	>MS.gene09871 ATGTCTGGCCGCGGAAAAGGAGGAAAGGGATTGGGAAAGGGAGGAGCAAAAAGGCACAGAAAGGTTCTTCGCGATAACATCCAAGGCATAACAAAGCCTGCGATTCGTCGTCTTGCAAGGCGTGGTGGTGTGAAGAGAATCAGTGGTCTCATCTATGAAGAGACTCGCGGTGTTCTCAAGATCTTCTTGGAGAACGTGATTCGTGATGCCGTTACTTACACTGAGCACGCTAGGAGGAAGACTGTTACCGCCATGGATGTTGTTTATGCTTTGAAGAGACAGGGAAGGACTCTCTACGGTTTCGGTGGTTAA
Protein sequence	>MS.gene09871.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG