AlfalfaGEDB | MS.gene31196

M. sativa cultivar XinJiangDaYe / MS.gene31196

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31196.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31196.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31196.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049208	MS.gene31196	0.808551	2.93E-50	-1.69E-46
MS.gene049756	MS.gene31196	0.806848	6.74E-50	-1.69E-46
MS.gene049857	MS.gene31196	0.814008	1.92E-51	-1.69E-46
MS.gene050277	MS.gene31196	0.801771	7.69E-49	-1.69E-46
MS.gene050317	MS.gene31196	0.827061	1.93E-54	-1.69E-46
MS.gene050752	MS.gene31196	0.806192	9.27E-50	-1.69E-46
MS.gene050838	MS.gene31196	0.842376	2.68E-58	-1.69E-46
MS.gene051233	MS.gene31196	0.804576	2.02E-49	-1.69E-46
MS.gene051243	MS.gene31196	0.801348	9.40E-49	-1.69E-46
MS.gene051245	MS.gene31196	0.80484	1.78E-49	-1.69E-46
MS.gene05134	MS.gene31196	0.801803	7.58E-49	-1.69E-46
MS.gene051451	MS.gene31196	0.812798	3.54E-51	-1.69E-46
MS.gene051746	MS.gene31196	0.813575	2.39E-51	-1.69E-46
MS.gene051748	MS.gene31196	0.8226	2.17E-53	-1.69E-46
MS.gene051756	MS.gene31196	0.805137	1.54E-49	-1.69E-46
MS.gene051757	MS.gene31196	0.801177	1.02E-48	-1.69E-46
MS.gene051903	MS.gene31196	0.830699	2.54E-55	-1.69E-46
MS.gene052335	MS.gene31196	0.80728	5.46E-50	-1.69E-46
MS.gene052434	MS.gene31196	0.807015	6.21E-50	-1.69E-46
MS.gene052856	MS.gene31196	0.806957	6.39E-50	-1.69E-46
MS.gene052947	MS.gene31196	0.821234	4.50E-53	-1.69E-46
MS.gene053058	MS.gene31196	0.805247	1.46E-49	-1.69E-46
MS.gene053109	MS.gene31196	0.807131	5.87E-50	-1.69E-46
MS.gene053381	MS.gene31196	0.816755	4.69E-52	-1.69E-46
MS.gene053952	MS.gene31196	0.83202	1.20E-55	-1.69E-46
MS.gene054403	MS.gene31196	0.800965	1.12E-48	-1.69E-46
MS.gene05469	MS.gene31196	0.812282	4.59E-51	-1.69E-46
MS.gene055162	MS.gene31196	0.829373	5.34E-55	-1.69E-46
MS.gene055311	MS.gene31196	0.841994	3.39E-58	-1.69E-46
MS.gene055313	MS.gene31196	0.816401	5.64E-52	-1.69E-46
MS.gene055314	MS.gene31196	0.819194	1.32E-52	-1.69E-46
MS.gene055424	MS.gene31196	0.82032	7.30E-53	-1.69E-46
MS.gene056580	MS.gene31196	0.821123	4.77E-53	-1.69E-46
MS.gene056601	MS.gene31196	0.816662	4.93E-52	-1.69E-46
MS.gene056631	MS.gene31196	0.805792	1.12E-49	-1.69E-46
MS.gene056787	MS.gene31196	0.802699	4.96E-49	-1.69E-46
MS.gene057057	MS.gene31196	0.833319	5.71E-56	-1.69E-46
MS.gene057094	MS.gene31196	0.813807	2.12E-51	-1.69E-46
MS.gene057234	MS.gene31196	0.835025	2.13E-56	-1.69E-46
MS.gene058055	MS.gene31196	0.804041	2.61E-49	-1.69E-46
MS.gene058238	MS.gene31196	0.808954	2.40E-50	-1.69E-46
MS.gene058646	MS.gene31196	0.802571	5.27E-49	-1.69E-46
MS.gene058654	MS.gene31196	0.810374	1.19E-50	-1.69E-46
MS.gene058906	MS.gene31196	0.825449	4.66E-54	-1.69E-46
MS.gene059171	MS.gene31196	0.816073	6.67E-52	-1.69E-46
MS.gene05978	MS.gene31196	0.800118	1.67E-48	-1.69E-46
MS.gene059898	MS.gene31196	0.80924	2.09E-50	-1.69E-46
MS.gene06001	MS.gene31196	0.830506	2.83E-55	-1.69E-46
MS.gene060463	MS.gene31196	0.846205	2.51E-59	-1.69E-46
MS.gene060465	MS.gene31196	0.852881	3.45E-61	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene31196	MS.gene62520	PPI
MS.gene31196	MS.gene060465	PPI
MS.gene31196	MS.gene08053	PPI
MS.gene31196	MS.gene018578	PPI
MS.gene31196	MS.gene035721	PPI
MS.gene31196	MS.gene061870	PPI
MS.gene31196	MS.gene018580	PPI
MS.gene31196	MS.gene004124	PPI
MS.gene028629	MS.gene31196	PPI
MS.gene31196	MS.gene88680	PPI
MS.gene31196	MS.gene072493	PPI
MS.gene31196	MS.gene04052	PPI
MS.gene035787	MS.gene31196	PPI
MS.gene04067	MS.gene31196	PPI
MS.gene71215	MS.gene31196	PPI
MS.gene31196	MS.gene84536	PPI
MS.gene31196	MS.gene035896	PPI
MS.gene31196	MS.gene061866	PPI
MS.gene31196	MS.gene64650	PPI
MS.gene31196	MS.gene048705	PPI
MS.gene31196	MS.gene061874	PPI
MS.gene31196	MS.gene061869	PPI
MS.gene071136	MS.gene31196	PPI
MS.gene31196	MS.gene04972	PPI
MS.gene31196	MS.gene070244	PPI
MS.gene31196	MS.gene004121	PPI
MS.gene88680	MS.gene31196	PPI
MS.gene056215	MS.gene31196	PPI
MS.gene31196	MS.gene009027	PPI
MS.gene31196	MS.gene061867	PPI
MS.gene31196	MS.gene04064	PPI
MS.gene31196	MS.gene008276	PPI
MS.gene31196	MS.gene30920	PPI
MS.gene31196	MS.gene05619	PPI
MS.gene31196	MS.gene04068	PPI
MS.gene31196	MS.gene035719	PPI
MS.gene31196	MS.gene061872	PPI
MS.gene31196	MS.gene89488	PPI
MS.gene31196	MS.gene66225	PPI
MS.gene31196	MS.gene70955	PPI
MS.gene31196	MS.gene79195	PPI
MS.gene31196	MS.gene61017	PPI
MS.gene31196	MS.gene04576	PPI
MS.gene31196	MS.gene09871	PPI
MS.gene31196	MS.gene012390	PPI
MS.gene31196	MS.gene08121	PPI
MS.gene31196	MS.gene64920	PPI
MS.gene31196	MS.gene064557	PPI
MS.gene30920	MS.gene31196	PPI
MS.gene061872	MS.gene31196	PPI
MS.gene31196	MS.gene04434	PPI
MS.gene31196	MS.gene04452	PPI
MS.gene31196	MS.gene04392	PPI
MS.gene31196	MS.gene89487	PPI
MS.gene31196	MS.gene010219	PPI
MS.gene31196	MS.gene04433	PPI
MS.gene31196	MS.gene040383	PPI
MS.gene070241	MS.gene31196	PPI
MS.gene31196	MS.gene038469	PPI
MS.gene31196	MS.gene016624	PPI
MS.gene061876	MS.gene31196	PPI
MS.gene31196	MS.gene73136	PPI
MS.gene31196	MS.gene049821	PPI
MS.gene012391	MS.gene31196	PPI
MS.gene31196	MS.gene022946	PPI
MS.gene070244	MS.gene31196	PPI
MS.gene31196	MS.gene05623	PPI
MS.gene060463	MS.gene31196	PPI
MS.gene31196	MS.gene58854	PPI
MS.gene31196	MS.gene035720	PPI
MS.gene060465	MS.gene31196	PPI
MS.gene31196	MS.gene035897	PPI
MS.gene31196	MS.gene049705	PPI
MS.gene31196	MS.gene070907	PPI
MS.gene31196	MS.gene05608	PPI
MS.gene31196	MS.gene68545	PPI
MS.gene31196	MS.gene070242	PPI
MS.gene31196	MS.gene88681	PPI
MS.gene31196	MS.gene82546	PPI
MS.gene061870	MS.gene31196	PPI
MS.gene31196	MS.gene048746	PPI
MS.gene31196	MS.gene60093	PPI
MS.gene035718	MS.gene31196	PPI
MS.gene31196	MS.gene08122	PPI
MS.gene31196	MS.gene09873	PPI
MS.gene31196	MS.gene028629	PPI
MS.gene31196	MS.gene035718	PPI
MS.gene31196	MS.gene022932	PPI
MS.gene009027	MS.gene31196	PPI
MS.gene31196	MS.gene043967	PPI
MS.gene31196	MS.gene010220	PPI
MS.gene78578	MS.gene31196	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31196.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene31196.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene31196.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene31196.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene31196.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene31196.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene31196.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene31196.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene31196.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene31196.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene31196.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene31196.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene31196.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene31196.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene31196.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene31196.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31196.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene31196.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene31196.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene31196.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene31196.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene31196.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene31196.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene31196.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene31196.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene31196.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene31196.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 33 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGACAAGGAAGGACTCTTTA+TGG	0.217305	2.1:-7439733	MS.gene31196:CDS
GGAAGGACTCTTTATGGATT+TGG	0.266178	2.1:-7439727	MS.gene31196:CDS
GAAGGACTCTTTATGGATTT+GGG	0.322097	2.1:-7439726	MS.gene31196:CDS
TTGTTGCGAATCAAGATGTC+TGG	0.363585	2.1:-7440024	None:intergenic
TGGAAAAGGTGGAAAGGGAT+TGG	0.405096	2.1:-7439998	MS.gene31196:CDS
GGAAAAGGTGGAAAGGGATT+GGG	0.428595	2.1:-7439997	MS.gene31196:CDS
GAAACCCGCTATTCGTCGTT+TGG	0.435441	2.1:-7439917	MS.gene31196:CDS
GGAAAGGGATTGGGAAAGGG+AGG	0.438545	2.1:-7439988	MS.gene31196:CDS
AAGATGTCTGGGCGTGGAAA+AGG	0.454402	2.1:-7440012	MS.gene31196:CDS
GGTGGAAAGGGATTGGGAAA+GGG	0.496399	2.1:-7439991	MS.gene31196:CDS
GTGCTTCGTGATAACATTCA+AGG	0.499787	2.1:-7439946	MS.gene31196:CDS
AGGTGGAAAGGGATTGGGAA+AGG	0.506594	2.1:-7439992	MS.gene31196:CDS
GAGAAAGACTGTTACCGCTA+TGG	0.523786	2.1:-7439776	MS.gene31196:CDS
CGTCGTTTGGCACGAAGAGG+AGG	0.535271	2.1:-7439904	MS.gene31196:CDS
TGTTGCGAATCAAGATGTCT+GGG	0.537404	2.1:-7440023	None:intergenic
CGTGCCAAACGACGAATAGC+GGG	0.539788	2.1:+7439913	None:intergenic
TGTTATCACGAAGCACCTTA+CGG	0.542642	2.1:+7439953	None:intergenic
CGAATCAAGATGTCTGGGCG+TGG	0.544605	2.1:-7440018	None:intergenic
TTACTTACACAGAGCACGCT+AGG	0.553235	2.1:-7439798	MS.gene31196:CDS
ATGTCTGGGCGTGGAAAAGG+TGG	0.568080	2.1:-7440009	MS.gene31196:CDS
AGGAGCAAAACGACACCGTA+AGG	0.575994	2.1:-7439968	MS.gene31196:CDS
GGGCGTGGAAAAGGTGGAAA+GGG	0.577024	2.1:-7440003	MS.gene31196:CDS
GGAGAATGTTATTCGTGATG+CGG	0.582811	2.1:-7439821	MS.gene31196:CDS
ATGCTTTGAAGAGACAAGGA+AGG	0.603293	2.1:-7439744	MS.gene31196:CDS
AAGGACTCTTTATGGATTTG+GGG	0.603730	2.1:-7439725	MS.gene31196:CDS
TCGTGCCAAACGACGAATAG+CGG	0.606775	2.1:+7439912	None:intergenic
TGGGCGTGGAAAAGGTGGAA+AGG	0.614761	2.1:-7440004	MS.gene31196:CDS
GGAGGAGTGAAGCGTATCAG+CGG	0.620100	2.1:-7439886	MS.gene31196:CDS
ATTCGTCGTTTGGCACGAAG+AGG	0.624143	2.1:-7439907	MS.gene31196:CDS
GTTTATGCTTTGAAGAGACA+AGG	0.627217	2.1:-7439748	MS.gene31196:CDS
AGGACTCTTTATGGATTTGG+GGG	0.645439	2.1:-7439724	MS.gene31196:CDS
AGCATAAACAACATCCATAG+CGG	0.701201	2.1:+7439762	None:intergenic
TTGATCTACGAAGAGACACG+TGG	0.703849	2.1:-7439862	MS.gene31196:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGGACTCTTTATGGATTTG+GGG	-	chr2.1:7440004-7440023	MS.gene31196:CDS	35.0%
	AGCATAAACAACATCCATAG+CGG	+	chr2.1:7439970-7439989	None:intergenic	35.0%
	GAAGGACTCTTTATGGATTT+GGG	-	chr2.1:7440003-7440022	MS.gene31196:CDS	35.0%
!!	GTTTATGCTTTGAAGAGACA+AGG	-	chr2.1:7439981-7440000	MS.gene31196:CDS	35.0%
	AGACAAGGAAGGACTCTTTA+TGG	-	chr2.1:7439996-7440015	MS.gene31196:CDS	40.0%
	AGGACTCTTTATGGATTTGG+GGG	-	chr2.1:7440005-7440024	MS.gene31196:CDS	40.0%
	GGAAGGACTCTTTATGGATT+TGG	-	chr2.1:7440002-7440021	MS.gene31196:CDS	40.0%
	GGAGAATGTTATTCGTGATG+CGG	-	chr2.1:7439908-7439927	MS.gene31196:CDS	40.0%
	GTGCTTCGTGATAACATTCA+AGG	-	chr2.1:7439783-7439802	MS.gene31196:CDS	40.0%
!	TGGCGTGTTGAAGATCTTTT+TGG	-	chr2.1:7439887-7439906	MS.gene31196:CDS	40.0%
!	TGTTATCACGAAGCACCTTA+CGG	+	chr2.1:7439779-7439798	None:intergenic	40.0%
!!	ATGCTTTGAAGAGACAAGGA+AGG	-	chr2.1:7439985-7440004	MS.gene31196:CDS	40.0%
	GAGAAAGACTGTTACCGCTA+TGG	-	chr2.1:7439953-7439972	MS.gene31196:CDS	45.0%
	GGAAAAGGTGGAAAGGGATT+GGG	-	chr2.1:7439732-7439751	MS.gene31196:CDS	45.0%
	TGGAAAAGGTGGAAAGGGAT+TGG	-	chr2.1:7439731-7439750	MS.gene31196:CDS	45.0%
	TTACTTACACAGAGCACGCT+AGG	-	chr2.1:7439931-7439950	MS.gene31196:CDS	45.0%
	TTGATCTACGAAGAGACACG+TGG	-	chr2.1:7439867-7439886	MS.gene31196:CDS	45.0%
	AGGAGCAAAACGACACCGTA+AGG	-	chr2.1:7439761-7439780	MS.gene31196:CDS	50.0%
	AGGTGGAAAGGGATTGGGAA+AGG	-	chr2.1:7439737-7439756	MS.gene31196:CDS	50.0%
	ATTCGTCGTTTGGCACGAAG+AGG	-	chr2.1:7439822-7439841	MS.gene31196:CDS	50.0%
	GAAACCCGCTATTCGTCGTT+TGG	-	chr2.1:7439812-7439831	MS.gene31196:CDS	50.0%
	GGTGGAAAGGGATTGGGAAA+GGG	-	chr2.1:7439738-7439757	MS.gene31196:CDS	50.0%
	TCGTGCCAAACGACGAATAG+CGG	+	chr2.1:7439820-7439839	None:intergenic	50.0%
	ATGTCTGGGCGTGGAAAAGG+TGG	-	chr2.1:7439720-7439739	MS.gene31196:CDS	55.0%
	CGTGCCAAACGACGAATAGC+GGG	+	chr2.1:7439819-7439838	None:intergenic	55.0%
	GGAAAGGGATTGGGAAAGGG+AGG	-	chr2.1:7439741-7439760	MS.gene31196:CDS	55.0%
	GGAGGAGTGAAGCGTATCAG+CGG	-	chr2.1:7439843-7439862	MS.gene31196:CDS	55.0%
	GGGCGTGGAAAAGGTGGAAA+GGG	-	chr2.1:7439726-7439745	MS.gene31196:CDS	55.0%
	TGGGCGTGGAAAAGGTGGAA+AGG	-	chr2.1:7439725-7439744	MS.gene31196:CDS	55.0%
	CGTCGTTTGGCACGAAGAGG+AGG	-	chr2.1:7439825-7439844	MS.gene31196:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	7439720	7440031	7439720	ID=MS.gene31196
chr2.1	mRNA	7439720	7440031	7439720	ID=MS.gene31196.t1;Parent=MS.gene31196
chr2.1	exon	7439720	7440031	7439720	ID=MS.gene31196.t1.exon1;Parent=MS.gene31196.t1
chr2.1	CDS	7439720	7440031	7439720	ID=cds.MS.gene31196.t1;Parent=MS.gene31196.t1

Gene Sequence	>MS.gene31196 ATGTCTGGGCGTGGAAAAGGTGGAAAGGGATTGGGAAAGGGAGGAGCAAAACGACACCGTAAGGTGCTTCGTGATAACATTCAAGGAATAACGAAACCCGCTATTCGTCGTTTGGCACGAAGAGGAGGAGTGAAGCGTATCAGCGGTTTGATCTACGAAGAGACACGTGGCGTGTTGAAGATCTTTTTGGAGAATGTTATTCGTGATGCGGTTACTTACACAGAGCACGCTAGGAGAAAGACTGTTACCGCTATGGATGTTGTTTATGCTTTGAAGAGACAAGGAAGGACTCTTTATGGATTTGGGGGTTAG
Protein sequence	>MS.gene31196.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG