AlfalfaGEDB | MS.gene035787

M. sativa cultivar XinJiangDaYe / MS.gene035787

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035787.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035787.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035787.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene035787	MS.gene012391	PPI
MS.gene035787	MS.gene73136	PPI
MS.gene035787	MS.gene049821	PPI
MS.gene035787	MS.gene78578	PPI
MS.gene035787	MS.gene04068	PPI
MS.gene035787	MS.gene022946	PPI
MS.gene035787	MS.gene035719	PPI
MS.gene035787	MS.gene05623	PPI
MS.gene035787	MS.gene89488	PPI
MS.gene056215	MS.gene035787	PPI
MS.gene035787	MS.gene58854	PPI
MS.gene035787	MS.gene66225	PPI
MS.gene035787	MS.gene035720	PPI
MS.gene035787	MS.gene70955	PPI
MS.gene035787	MS.gene61017	PPI
MS.gene035787	MS.gene09871	PPI
MS.gene035787	MS.gene31196	PPI
MS.gene035787	MS.gene012390	PPI
MS.gene061876	MS.gene035787	PPI
MS.gene035787	MS.gene08121	PPI
MS.gene035787	MS.gene04067	PPI
MS.gene035787	MS.gene064557	PPI
MS.gene035787	MS.gene049705	PPI
MS.gene035787	MS.gene04434	PPI
MS.gene035787	MS.gene070907	PPI
MS.gene035787	MS.gene04392	PPI
MS.gene035787	MS.gene060463	PPI
MS.gene060463	MS.gene035787	PPI
MS.gene035787	MS.gene010219	PPI
MS.gene035787	MS.gene04433	PPI
MS.gene035787	MS.gene05608	PPI
MS.gene035787	MS.gene040383	PPI
MS.gene035787	MS.gene68545	PPI
MS.gene035787	MS.gene070242	PPI
MS.gene035787	MS.gene016624	PPI
MS.gene035787	MS.gene82546	PPI
MS.gene035787	MS.gene048746	PPI
MS.gene035787	MS.gene71215	PPI
MS.gene035787	MS.gene09873	PPI
MS.gene070241	MS.gene035787	PPI
MS.gene035787	MS.gene028629	PPI
MS.gene035787	MS.gene043967	PPI
MS.gene035787	MS.gene060465	PPI
MS.gene035787	MS.gene08053	PPI
MS.gene035787	MS.gene035897	PPI
MS.gene012391	MS.gene035787	PPI
MS.gene035787	MS.gene035721	PPI
MS.gene035787	MS.gene061870	PPI
MS.gene035787	MS.gene070241	PPI
MS.gene035787	MS.gene072493	PPI
MS.gene035787	MS.gene84536	PPI
MS.gene035787	MS.gene035896	PPI
MS.gene035787	MS.gene88681	PPI
MS.gene035787	MS.gene64650	PPI
MS.gene035787	MS.gene60093	PPI
MS.gene035787	MS.gene08122	PPI
MS.gene035787	MS.gene061869	PPI
MS.gene035787	MS.gene035718	PPI
MS.gene035787	MS.gene022932	PPI
MS.gene035787	MS.gene010220	PPI
MS.gene035787	MS.gene04972	PPI
MS.gene035787	MS.gene004121	PPI
MS.gene035787	MS.gene62520	PPI
MS.gene035787	MS.gene018578	PPI
MS.gene035787	MS.gene009027	PPI
MS.gene035787	MS.gene061867	PPI
MS.gene035787	MS.gene04064	PPI
MS.gene035787	MS.gene018580	PPI
MS.gene035787	MS.gene004124	PPI
MS.gene035787	MS.gene071136	PPI
MS.gene071136	MS.gene035787	PPI
MS.gene035787	MS.gene88680	PPI
MS.gene035787	MS.gene30920	PPI
MS.gene035787	MS.gene05619	PPI
MS.gene78578	MS.gene035787	PPI
MS.gene035787	MS.gene04052	PPI
MS.gene035787	MS.gene061872	PPI
MS.gene035787	MS.gene061866	PPI
MS.gene035787	MS.gene79195	PPI
MS.gene035787	MS.gene048705	PPI
MS.gene035787	MS.gene061876	PPI
MS.gene035787	MS.gene061874	PPI
MS.gene035787	MS.gene04576	PPI
MS.gene035787	MS.gene056215	PPI
MS.gene035787	MS.gene64920	PPI
MS.gene04067	MS.gene035787	PPI
MS.gene71215	MS.gene035787	PPI
MS.gene035787	MS.gene04452	PPI
MS.gene035787	MS.gene070244	PPI
MS.gene035787	MS.gene89487	PPI
MS.gene035787	MS.gene038469	PPI
MS.gene035787	MS.gene008276	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035787.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035787.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035787.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035787.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035787.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035787.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035787.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035787.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035787.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035787.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035787.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035787.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035787.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035787.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035787.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene035787.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035787.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035787.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035787.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035787.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035787.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035787.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035787.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035787.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035787.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035787.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035787.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 30 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGACAAGGAAGAACGCTTTA+TGG	0.218720	3.2:-46251625	MS.gene035787:CDS
GGAAGAACGCTTTATGGATT+CGG	0.427609	3.2:-46251619	MS.gene035787:CDS
GGGAGGTAAGGGACTAGGAA+AGG	0.428277	3.2:-46251884	MS.gene035787:CDS
GGAGGTAAGGGACTAGGAAA+GGG	0.452874	3.2:-46251883	MS.gene035787:CDS
CTCTTGAAAATGTCAGGTCG+TGG	0.456977	3.2:-46251910	None:intergenic
CTCGCCAATCGACGAATCGC+TGG	0.484651	3.2:+46251805	None:intergenic
AGGTCGTGGAAAGGGAGGTA+AGG	0.492819	3.2:-46251896	MS.gene035787:CDS
AGTTCTTCGTGACAACATCC+AGG	0.501637	3.2:-46251839	MS.gene035787:CDS
AAAGCCAGCGATTCGTCGAT+TGG	0.503407	3.2:-46251809	MS.gene035787:CDS
CGTCGATTGGCGAGAAGAGG+TGG	0.519231	3.2:-46251796	MS.gene035787:CDS
GGAAAGGGAGGTAAGGGACT+AGG	0.521051	3.2:-46251889	MS.gene035787:CDS
AAAATGTCAGGTCGTGGAAA+GGG	0.538992	3.2:-46251904	MS.gene035787:CDS
GAGGAAGACGGTTACAGCCA+TGG	0.550679	3.2:-46251668	MS.gene035787:CDS
GGTAAGGGACTAGGAAAGGG+AGG	0.563878	3.2:-46251880	MS.gene035787:CDS
GGTCGTGGAAAGGGAGGTAA+GGG	0.564245	3.2:-46251895	MS.gene035787:CDS
GTCTATGCTTTGAAGAGACA+AGG	0.570048	3.2:-46251640	MS.gene035787:CDS
AATCAGCGGTTTGATCTATG+AGG	0.581523	3.2:-46251764	MS.gene035787:CDS
GAAAATGTCAGGTCGTGGAA+AGG	0.584382	3.2:-46251905	None:intergenic
TTACTTACACTGAGCATGCA+AGG	0.587365	3.2:-46251690	MS.gene035787:CDS
TCGCTGGCTTTGTGATACCC+TGG	0.588577	3.2:+46251821	None:intergenic
AGAACGCTTTATGGATTCGG+AGG	0.604365	3.2:-46251616	MS.gene035787:CDS
ATTCGTCGATTGGCGAGAAG+AGG	0.612986	3.2:-46251799	MS.gene035787:CDS
CAAAGCATAGACAACATCCA+TGG	0.629842	3.2:+46251651	None:intergenic
GGAGAATGTGATTCGTGATG+CGG	0.636428	3.2:-46251713	MS.gene035787:CDS
TTGATCTATGAGGAAACAAG+AGG	0.641491	3.2:-46251754	MS.gene035787:CDS
ATGTCAGGTCGTGGAAAGGG+AGG	0.643397	3.2:-46251901	MS.gene035787:CDS
TGAGCATGCAAGGAGGAAGA+CGG	0.657376	3.2:-46251680	MS.gene035787:CDS
GGTGGTGTTAAGAGAATCAG+CGG	0.661394	3.2:-46251778	MS.gene035787:CDS
GTTCTTCGTGACAACATCCA+GGG	0.666413	3.2:-46251838	MS.gene035787:CDS
CTTACACTGAGCATGCAAGG+AGG	0.756108	3.2:-46251687	MS.gene035787:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGGAGTTCTTAAGATTTTCT+TGG	-	chr3.2:46251779-46251798	MS.gene035787:CDS	30.0%
	TTGATCTATGAGGAAACAAG+AGG	-	chr3.2:46251759-46251778	MS.gene035787:CDS	35.0%
	AATCAGCGGTTTGATCTATG+AGG	-	chr3.2:46251749-46251768	MS.gene035787:CDS	40.0%
	AGACAAGGAAGAACGCTTTA+TGG	-	chr3.2:46251888-46251907	MS.gene035787:CDS	40.0%
	CAAAGCATAGACAACATCCA+TGG	+	chr3.2:46251865-46251884	None:intergenic	40.0%
	GGAAGAACGCTTTATGGATT+CGG	-	chr3.2:46251894-46251913	MS.gene035787:CDS	40.0%
	TTACTTACACTGAGCATGCA+AGG	-	chr3.2:46251823-46251842	MS.gene035787:CDS	40.0%
!!	GTCTATGCTTTGAAGAGACA+AGG	-	chr3.2:46251873-46251892	MS.gene035787:CDS	40.0%
	AGAACGCTTTATGGATTCGG+AGG	-	chr3.2:46251897-46251916	MS.gene035787:CDS	45.0%
	AGTTCTTCGTGACAACATCC+AGG	-	chr3.2:46251674-46251693	MS.gene035787:CDS	45.0%
	GGAGAATGTGATTCGTGATG+CGG	-	chr3.2:46251800-46251819	MS.gene035787:CDS	45.0%
	GTTCTTCGTGACAACATCCA+GGG	-	chr3.2:46251675-46251694	MS.gene035787:CDS	45.0%
!	GGTGGTGTTAAGAGAATCAG+CGG	-	chr3.2:46251735-46251754	MS.gene035787:CDS	45.0%
	AAAGCCAGCGATTCGTCGAT+TGG	-	chr3.2:46251704-46251723	MS.gene035787:CDS	50.0%
	CTTACACTGAGCATGCAAGG+AGG	-	chr3.2:46251826-46251845	MS.gene035787:CDS	50.0%
	GGAGGTAAGGGACTAGGAAA+GGG	-	chr3.2:46251630-46251649	MS.gene035787:CDS	50.0%
	TGAGCATGCAAGGAGGAAGA+CGG	-	chr3.2:46251833-46251852	MS.gene035787:CDS	50.0%
!	ATTCGTCGATTGGCGAGAAG+AGG	-	chr3.2:46251714-46251733	MS.gene035787:CDS	50.0%
	AGGTCGTGGAAAGGGAGGTA+AGG	-	chr3.2:46251617-46251636	MS.gene035787:CDS	55.0%
	ATGTCAGGTCGTGGAAAGGG+AGG	-	chr3.2:46251612-46251631	MS.gene035787:CDS	55.0%
	GAGGAAGACGGTTACAGCCA+TGG	-	chr3.2:46251845-46251864	MS.gene035787:CDS	55.0%
	GGAAAGGGAGGTAAGGGACT+AGG	-	chr3.2:46251624-46251643	MS.gene035787:CDS	55.0%
	GGGAGGTAAGGGACTAGGAA+AGG	-	chr3.2:46251629-46251648	MS.gene035787:CDS	55.0%
	GGTAAGGGACTAGGAAAGGG+AGG	-	chr3.2:46251633-46251652	MS.gene035787:CDS	55.0%
	GGTCGTGGAAAGGGAGGTAA+GGG	-	chr3.2:46251618-46251637	MS.gene035787:CDS	55.0%
!!	TCGCTGGCTTTGTGATACCC+TGG	+	chr3.2:46251695-46251714	None:intergenic	55.0%
	CTCGCCAATCGACGAATCGC+TGG	+	chr3.2:46251711-46251730	None:intergenic	60.0%
!	CGTCGATTGGCGAGAAGAGG+TGG	-	chr3.2:46251717-46251736	MS.gene035787:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	46251612	46251923	46251612	ID=MS.gene035787
chr3.2	mRNA	46251612	46251923	46251612	ID=MS.gene035787.t1;Parent=MS.gene035787
chr3.2	exon	46251612	46251923	46251612	ID=MS.gene035787.t1.exon1;Parent=MS.gene035787.t1
chr3.2	CDS	46251612	46251923	46251612	ID=cds.MS.gene035787.t1;Parent=MS.gene035787.t1

Gene Sequence	>MS.gene035787 ATGTCAGGTCGTGGAAAGGGAGGTAAGGGACTAGGAAAGGGAGGTGCAAAGAGACATCGAAAAGTTCTTCGTGACAACATCCAGGGTATCACAAAGCCAGCGATTCGTCGATTGGCGAGAAGAGGTGGTGTTAAGAGAATCAGCGGTTTGATCTATGAGGAAACAAGAGGAGTTCTTAAGATTTTCTTGGAGAATGTGATTCGTGATGCGGTTACTTACACTGAGCATGCAAGGAGGAAGACGGTTACAGCCATGGATGTTGTCTATGCTTTGAAGAGACAAGGAAGAACGCTTTATGGATTCGGAGGTTGA
Protein sequence	>MS.gene035787.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG