AlfalfaGEDB | MS.gene061876

M. sativa cultivar XinJiangDaYe / MS.gene061876

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061876.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061876.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061876.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene070241	MS.gene061876	PPI
MS.gene056215	MS.gene061876	PPI
MS.gene061876	MS.gene04052	PPI
MS.gene061876	MS.gene061866	PPI
MS.gene061876	MS.gene64650	PPI
MS.gene061876	MS.gene79195	PPI
MS.gene061876	MS.gene048705	PPI
MS.gene061876	MS.gene061874	PPI
MS.gene061876	MS.gene04576	PPI
MS.gene061876	MS.gene056215	PPI
MS.gene012391	MS.gene061876	PPI
MS.gene061876	MS.gene061869	PPI
MS.gene061876	MS.gene64920	PPI
MS.gene060463	MS.gene061876	PPI
MS.gene061876	MS.gene035787	PPI
MS.gene061876	MS.gene04452	PPI
MS.gene061876	MS.gene070244	PPI
MS.gene061876	MS.gene038469	PPI
MS.gene061876	MS.gene061867	PPI
MS.gene061876	MS.gene008276	PPI
MS.gene061876	MS.gene30920	PPI
MS.gene061876	MS.gene012391	PPI
MS.gene061876	MS.gene05619	PPI
MS.gene061876	MS.gene73136	PPI
MS.gene061876	MS.gene049821	PPI
MS.gene061876	MS.gene78578	PPI
MS.gene061876	MS.gene04068	PPI
MS.gene061876	MS.gene022946	PPI
MS.gene061876	MS.gene035719	PPI
MS.gene061876	MS.gene05623	PPI
MS.gene061876	MS.gene061872	PPI
MS.gene061876	MS.gene89488	PPI
MS.gene061876	MS.gene58854	PPI
MS.gene061876	MS.gene66225	PPI
MS.gene061876	MS.gene035720	PPI
MS.gene061876	MS.gene70955	PPI
MS.gene061876	MS.gene61017	PPI
MS.gene061876	MS.gene09871	PPI
MS.gene061876	MS.gene012390	PPI
MS.gene061876	MS.gene08121	PPI
MS.gene061876	MS.gene04067	PPI
MS.gene061876	MS.gene064557	PPI
MS.gene061876	MS.gene049705	PPI
MS.gene061876	MS.gene04434	PPI
MS.gene061876	MS.gene070907	PPI
MS.gene061876	MS.gene04392	PPI
MS.gene061876	MS.gene060463	PPI
MS.gene061876	MS.gene89487	PPI
MS.gene061876	MS.gene010219	PPI
MS.gene061876	MS.gene04433	PPI
MS.gene061876	MS.gene040383	PPI
MS.gene061876	MS.gene68545	PPI
MS.gene061876	MS.gene070242	PPI
MS.gene061876	MS.gene016624	PPI
MS.gene061876	MS.gene048746	PPI
MS.gene061876	MS.gene71215	PPI
MS.gene061876	MS.gene028629	PPI
MS.gene71215	MS.gene061876	PPI
MS.gene061876	MS.gene043967	PPI
MS.gene061876	MS.gene31196	PPI
MS.gene061876	MS.gene060465	PPI
MS.gene061876	MS.gene08053	PPI
MS.gene061876	MS.gene035897	PPI
MS.gene061876	MS.gene035721	PPI
MS.gene061876	MS.gene061870	PPI
MS.gene071136	MS.gene061876	PPI
MS.gene061876	MS.gene05608	PPI
MS.gene78578	MS.gene061876	PPI
MS.gene061876	MS.gene070241	PPI
MS.gene061876	MS.gene072493	PPI
MS.gene061876	MS.gene84536	PPI
MS.gene061876	MS.gene035896	PPI
MS.gene061876	MS.gene88681	PPI
MS.gene061876	MS.gene82546	PPI
MS.gene061876	MS.gene60093	PPI
MS.gene061876	MS.gene08122	PPI
MS.gene061876	MS.gene09873	PPI
MS.gene035787	MS.gene061876	PPI
MS.gene04067	MS.gene061876	PPI
MS.gene061876	MS.gene035718	PPI
MS.gene061876	MS.gene022932	PPI
MS.gene061876	MS.gene010220	PPI
MS.gene061876	MS.gene04972	PPI
MS.gene061876	MS.gene004121	PPI
MS.gene061876	MS.gene62520	PPI
MS.gene061876	MS.gene018578	PPI
MS.gene061876	MS.gene009027	PPI
MS.gene061876	MS.gene04064	PPI
MS.gene061876	MS.gene018580	PPI
MS.gene061876	MS.gene004124	PPI
MS.gene061876	MS.gene071136	PPI
MS.gene061876	MS.gene88680	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061876.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061876.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061876.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061876.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061876.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061876.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061876.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061876.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061876.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061876.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061876.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061876.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061876.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061876.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061876.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene061876.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061876.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061876.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061876.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061876.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061876.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061876.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061876.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061876.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061876.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061876.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061876.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 32 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGTGTTCTCAAGATCTTCT+TGG	0.324000	7.4:+10649556	MS.gene061876:CDS
GGAAAGGGATTGGGAAAGGG+AGG	0.412951	7.4:+10649410	MS.gene061876:CDS
TCGCAGGCTTTGTTATGCCT+TGG	0.436279	7.4:-10649469	None:intergenic
AAAATGTCTGGCCGCGGAAA+AGG	0.446222	7.4:+10649386	None:intergenic
TGTTTATGCTTTGAAGAGAC+AGG	0.447929	7.4:+10649649	MS.gene061876:CDS
GGAGGAAAGGGATTGGGAAA+GGG	0.462159	7.4:+10649407	MS.gene061876:CDS
GGAAGGACTCTCTACGGTTT+CGG	0.462635	7.4:+10649671	MS.gene061876:CDS
AGGAGGAAAGGGATTGGGAA+AGG	0.466050	7.4:+10649406	MS.gene061876:CDS
GGAAAAGGAGGAAAGGGATT+GGG	0.479340	7.4:+10649401	MS.gene061876:CDS
CGGAAAAGGAGGAAAGGGAT+TGG	0.482086	7.4:+10649400	MS.gene061876:CDS
CTGCGATTCGTCGTCTTGCA+AGG	0.502831	7.4:+10649486	MS.gene061876:CDS
GGCCGCGGAAAAGGAGGAAA+GGG	0.550993	7.4:+10649395	MS.gene061876:CDS
CTTGCAAGACGACGAATCGC+AGG	0.552192	7.4:-10649485	None:intergenic
AGGAGCAAAGAGGCACAGAA+AGG	0.564264	7.4:+10649430	MS.gene061876:CDS
TTACTTACACTGAGCACGCT+AGG	0.572434	7.4:+10649600	MS.gene061876:CDS
ATGTCTGGCCGCGGAAAAGG+AGG	0.578066	7.4:+10649389	MS.gene061876:CDS
GAGGAAGACTGTTACCGCCA+TGG	0.588179	7.4:+10649622	MS.gene061876:CDS
TGGCCGCGGAAAAGGAGGAA+AGG	0.594714	7.4:+10649394	MS.gene061876:CDS
AGACAGGGAAGGACTCTCTA+CGG	0.604561	7.4:+10649665	MS.gene061876:CDS
AGGACTCTCTACGGTTTCGG+TGG	0.607233	7.4:+10649674	MS.gene061876:CDS
CAAAGCATAAACAACATCCA+TGG	0.612707	7.4:-10649639	None:intergenic
CGTCGTCTTGCAAGGCGTGG+TGG	0.615268	7.4:+10649494	MS.gene061876:CDS
GTTTATGCTTTGAAGAGACA+GGG	0.618996	7.4:+10649650	MS.gene061876:CDS
ATGCTTTGAAGAGACAGGGA+AGG	0.619457	7.4:+10649654	MS.gene061876:CDS
ATTCGTCGTCTTGCAAGGCG+TGG	0.619919	7.4:+10649491	MS.gene061876:CDS
GTTCTTCGCGATAACATCCA+AGG	0.633409	7.4:+10649452	MS.gene061876:CDS
AGCGTGCTCAGTGTAAGTAA+CGG	0.650649	7.4:-10649597	None:intergenic
CTTACACTGAGCACGCTAGG+AGG	0.678435	7.4:+10649603	MS.gene061876:CDS
TGGGAAAGGGAGGAGCAAAG+AGG	0.685544	7.4:+10649420	MS.gene061876:CDS
GGTGGTGTGAAGAGAATCAG+TGG	0.694412	7.4:+10649512	MS.gene061876:CDS
CTCATCTATGAAGAGACTCG+CGG	0.697662	7.4:+10649536	MS.gene061876:CDS
AGCATAAACAACATCCATGG+CGG	0.725411	7.4:-10649636	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CAAAGCATAAACAACATCCA+TGG	-	chr7.4:10649642-10649661	None:intergenic	35.0%
!!	GTTTATGCTTTGAAGAGACA+GGG	+	chr7.4:10649650-10649669	MS.gene061876:CDS	35.0%
!!	TGTTTATGCTTTGAAGAGAC+AGG	+	chr7.4:10649649-10649668	MS.gene061876:CDS	35.0%
	AGCATAAACAACATCCATGG+CGG	-	chr7.4:10649639-10649658	None:intergenic	40.0%
	AGCGTGCTCAGTGTAAGTAA+CGG	-	chr7.4:10649600-10649619	None:intergenic	45.0%
	CGGTGTTCTCAAGATCTTCT+TGG	+	chr7.4:10649556-10649575	MS.gene061876:CDS	45.0%
	CTCATCTATGAAGAGACTCG+CGG	+	chr7.4:10649536-10649555	MS.gene061876:CDS	45.0%
	GGAAAAGGAGGAAAGGGATT+GGG	+	chr7.4:10649401-10649420	MS.gene061876:CDS	45.0%
	GTTCTTCGCGATAACATCCA+AGG	+	chr7.4:10649452-10649471	MS.gene061876:CDS	45.0%
	TTACTTACACTGAGCACGCT+AGG	+	chr7.4:10649600-10649619	MS.gene061876:CDS	45.0%
!!	ATGCTTTGAAGAGACAGGGA+AGG	+	chr7.4:10649654-10649673	MS.gene061876:CDS	45.0%
	AGACAGGGAAGGACTCTCTA+CGG	+	chr7.4:10649665-10649684	MS.gene061876:CDS	50.0%
	AGGAGGAAAGGGATTGGGAA+AGG	+	chr7.4:10649406-10649425	MS.gene061876:CDS	50.0%
	CGGAAAAGGAGGAAAGGGAT+TGG	+	chr7.4:10649400-10649419	MS.gene061876:CDS	50.0%
	GGAAGGACTCTCTACGGTTT+CGG	+	chr7.4:10649671-10649690	MS.gene061876:CDS	50.0%
	GGAGGAAAGGGATTGGGAAA+GGG	+	chr7.4:10649407-10649426	MS.gene061876:CDS	50.0%
	GGTGGTGTGAAGAGAATCAG+TGG	+	chr7.4:10649512-10649531	MS.gene061876:CDS	50.0%
!	AGGAGCAAAGAGGCACAGAA+AGG	+	chr7.4:10649430-10649449	MS.gene061876:CDS	50.0%
!	ATCCCTTTCCTCCTTTTCCG+CGG	-	chr7.4:10649400-10649419	None:intergenic	50.0%
!!	TCGCAGGCTTTGTTATGCCT+TGG	-	chr7.4:10649472-10649491	None:intergenic	50.0%
	AGGACTCTCTACGGTTTCGG+TGG	+	chr7.4:10649674-10649693	MS.gene061876:CDS	55.0%
	ATTCGTCGTCTTGCAAGGCG+TGG	+	chr7.4:10649491-10649510	MS.gene061876:CDS	55.0%
	CTGCGATTCGTCGTCTTGCA+AGG	+	chr7.4:10649486-10649505	MS.gene061876:CDS	55.0%
	CTTACACTGAGCACGCTAGG+AGG	+	chr7.4:10649603-10649622	MS.gene061876:CDS	55.0%
	CTTGCAAGACGACGAATCGC+AGG	-	chr7.4:10649488-10649507	None:intergenic	55.0%
	GAGGAAGACTGTTACCGCCA+TGG	+	chr7.4:10649622-10649641	MS.gene061876:CDS	55.0%
	GGAAAGGGATTGGGAAAGGG+AGG	+	chr7.4:10649410-10649429	MS.gene061876:CDS	55.0%
	TGGGAAAGGGAGGAGCAAAG+AGG	+	chr7.4:10649420-10649439	MS.gene061876:CDS	55.0%
	ATGTCTGGCCGCGGAAAAGG+AGG	+	chr7.4:10649389-10649408	MS.gene061876:CDS	60.0%
	GGCCGCGGAAAAGGAGGAAA+GGG	+	chr7.4:10649395-10649414	MS.gene061876:CDS	60.0%
	TGGCCGCGGAAAAGGAGGAA+AGG	+	chr7.4:10649394-10649413	MS.gene061876:CDS	60.0%
	CGTCGTCTTGCAAGGCGTGG+TGG	+	chr7.4:10649494-10649513	MS.gene061876:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	10649389	10649700	10649389	ID=MS.gene061876
chr7.4	mRNA	10649389	10649700	10649389	ID=MS.gene061876.t1;Parent=MS.gene061876
chr7.4	exon	10649389	10649700	10649389	ID=MS.gene061876.t1.exon1;Parent=MS.gene061876.t1
chr7.4	CDS	10649389	10649700	10649389	ID=cds.MS.gene061876.t1;Parent=MS.gene061876.t1

Gene Sequence	>MS.gene061876 ATGTCTGGCCGCGGAAAAGGAGGAAAGGGATTGGGAAAGGGAGGAGCAAAGAGGCACAGAAAGGTTCTTCGCGATAACATCCAAGGCATAACAAAGCCTGCGATTCGTCGTCTTGCAAGGCGTGGTGGTGTGAAGAGAATCAGTGGTCTCATCTATGAAGAGACTCGCGGTGTTCTCAAGATCTTCTTGGAGAACGTGATTCGTGATGCCGTTACTTACACTGAGCACGCTAGGAGGAAGACTGTTACCGCCATGGATGTTGTTTATGCTTTGAAGAGACAGGGAAGGACTCTCTACGGTTTCGGTGGTTAA
Protein sequence	>MS.gene061876.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG