AlfalfaGEDB | MS.gene022932

M. sativa cultivar XinJiangDaYe / MS.gene022932

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene022932.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene022932.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene022932.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene022932	MS.gene072493	PPI
MS.gene022932	MS.gene84536	PPI
MS.gene022932	MS.gene035896	PPI
MS.gene022932	MS.gene64650	PPI
MS.gene022932	MS.gene60093	PPI
MS.gene022932	MS.gene08122	PPI
MS.gene08121	MS.gene022932	PPI
MS.gene022932	MS.gene061869	PPI
MS.gene022932	MS.gene010220	PPI
MS.gene035718	MS.gene022932	PPI
MS.gene035896	MS.gene022932	PPI
MS.gene022932	MS.gene04972	PPI
MS.gene022932	MS.gene004121	PPI
MS.gene022932	MS.gene018578	PPI
MS.gene009027	MS.gene022932	PPI
MS.gene022932	MS.gene061867	PPI
MS.gene022932	MS.gene04064	PPI
MS.gene022932	MS.gene018580	PPI
MS.gene78578	MS.gene022932	PPI
MS.gene022932	MS.gene004124	PPI
MS.gene022932	MS.gene05619	PPI
MS.gene043967	MS.gene022932	PPI
MS.gene022932	MS.gene04052	PPI
MS.gene028629	MS.gene022932	PPI
MS.gene022932	MS.gene89488	PPI
MS.gene022932	MS.gene061866	PPI
MS.gene04433	MS.gene022932	PPI
MS.gene022932	MS.gene66225	PPI
MS.gene022932	MS.gene79195	PPI
MS.gene022932	MS.gene048705	PPI
MS.gene022932	MS.gene061874	PPI
MS.gene022932	MS.gene04576	PPI
MS.gene04067	MS.gene022932	PPI
MS.gene71215	MS.gene022932	PPI
MS.gene061870	MS.gene022932	PPI
MS.gene022932	MS.gene012390	PPI
MS.gene66225	MS.gene022932	PPI
MS.gene70955	MS.gene022932	PPI
MS.gene071136	MS.gene022932	PPI
MS.gene040383	MS.gene022932	PPI
MS.gene070242	MS.gene022932	PPI
MS.gene022932	MS.gene016624	PPI
MS.gene022932	MS.gene008276	PPI
MS.gene035721	MS.gene022932	PPI
MS.gene022932	MS.gene73136	PPI
MS.gene056215	MS.gene022932	PPI
MS.gene064557	MS.gene022932	PPI
MS.gene022932	MS.gene049821	PPI
MS.gene038469	MS.gene022932	PPI
MS.gene022932	MS.gene04068	PPI
MS.gene022932	MS.gene022946	PPI
MS.gene022932	MS.gene05623	PPI
MS.gene04452	MS.gene022932	PPI
MS.gene022932	MS.gene035720	PPI
MS.gene022932	MS.gene61017	PPI
MS.gene035787	MS.gene022932	PPI
MS.gene022932	MS.gene09871	PPI
MS.gene89487	MS.gene022932	PPI
MS.gene022932	MS.gene049705	PPI
MS.gene035719	MS.gene022932	PPI
MS.gene022932	MS.gene04434	PPI
MS.gene58854	MS.gene022932	PPI
MS.gene022932	MS.gene070907	PPI
MS.gene022932	MS.gene04392	PPI
MS.gene022932	MS.gene010219	PPI
MS.gene022932	MS.gene05608	PPI
MS.gene30920	MS.gene022932	PPI
MS.gene010220	MS.gene022932	PPI
MS.gene08053	MS.gene022932	PPI
MS.gene022932	MS.gene68545	PPI
MS.gene061872	MS.gene022932	PPI
MS.gene022932	MS.gene070242	PPI
MS.gene88680	MS.gene022932	PPI
MS.gene022932	MS.gene82546	PPI
MS.gene070241	MS.gene022932	PPI
MS.gene022932	MS.gene048746	PPI
MS.gene022932	MS.gene09873	PPI
MS.gene061876	MS.gene022932	PPI
MS.gene012391	MS.gene022932	PPI
MS.gene88681	MS.gene022932	PPI
MS.gene022932	MS.gene043967	PPI
MS.gene070244	MS.gene022932	PPI
MS.gene64920	MS.gene022932	PPI
MS.gene060463	MS.gene022932	PPI
MS.gene022932	MS.gene62520	PPI
MS.gene022932	MS.gene035897	PPI
MS.gene022932	MS.gene035721	PPI
MS.gene31196	MS.gene022932	PPI
MS.gene060465	MS.gene022932	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene022932.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene022932.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene022932.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene022932.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene022932.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene022932.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene022932.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene022932.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene022932.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene022932.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene022932.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene022932.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene022932.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene022932.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene022932.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene022932.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene022932.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene022932.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene022932.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene022932.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene022932.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene022932.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene022932.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene022932.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene022932.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene022932.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene022932.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 36 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAAGAACCCTCTATGGATT+TGG	0.399537	7.2:-47664041	MS.gene022932:CDS
AGGTGTCTTGAAGATCTTCT+TGG	0.405705	7.2:-47664156	MS.gene022932:CDS
AGGCAAGGAAGAACCCTCTA+TGG	0.410062	7.2:-47664047	MS.gene022932:CDS
GGAGGTAAGGGACTTGGAAA+AGG	0.451621	7.2:-47664305	MS.gene022932:CDS
CTTGCCAATCGACGAATCGC+AGG	0.455924	7.2:+47664227	None:intergenic
TTCAACCGCCAAATCCATAG+AGG	0.460668	7.2:+47664033	None:intergenic
GGAAAGGGAGGTAAGGGACT+TGG	0.470633	7.2:-47664311	MS.gene022932:CDS
CAAGCCTGCGATTCGTCGAT+TGG	0.481072	7.2:-47664231	MS.gene022932:CDS
AGGTCGTGGAAAGGGAGGTA+AGG	0.492819	7.2:-47664318	MS.gene022932:CDS
CAATCGACGAATCGCAGGCT+TGG	0.503635	7.2:+47664232	None:intergenic
GAAGATCTTCAAGACACCTC+TGG	0.510902	7.2:+47664160	None:intergenic
ATGTTGTCTATGCGCTTAAG+AGG	0.513475	7.2:-47664067	MS.gene022932:CDS
AGTTCTCCGTGATAACATCC+AGG	0.528902	7.2:-47664261	MS.gene022932:CDS
GTCGCGAAAATGTCAGGTCG+TGG	0.532522	7.2:-47664332	None:intergenic
AAAATGTCAGGTCGTGGAAA+GGG	0.539338	7.2:-47664326	MS.gene022932:CDS
GAAAATGTCAGGTCGTGGAA+AGG	0.540836	7.2:-47664327	None:intergenic
AGAACCCTCTATGGATTTGG+CGG	0.541328	7.2:-47664038	MS.gene022932:CDS
GTCTATGCGCTTAAGAGGCA+AGG	0.561213	7.2:-47664062	MS.gene022932:CDS
GAGGAAGACGGTGACAGCCA+TGG	0.561434	7.2:-47664090	MS.gene022932:CDS
GGTCGTGGAAAGGGAGGTAA+GGG	0.564245	7.2:-47664317	MS.gene022932:CDS
AAGGAGGTGCAAAGAGACAT+AGG	0.568952	7.2:-47664286	MS.gene022932:CDS
TCGCAGGCTTGGTGATACCC+TGG	0.588643	7.2:+47664243	None:intergenic
CGTCGATTGGCAAGAAGAGG+TGG	0.589209	7.2:-47664218	MS.gene022932:CDS
TTACGTACACTGAGCACGCA+AGG	0.589661	7.2:-47664112	MS.gene022932:CDS
TGATACCCTGGATGTTATCA+CGG	0.616496	7.2:+47664255	None:intergenic
ATTCGTCGATTGGCAAGAAG+AGG	0.623397	7.2:-47664221	MS.gene022932:CDS
TCAACCGCCAAATCCATAGA+GGG	0.625286	7.2:+47664034	None:intergenic
ATGTCAGGTCGTGGAAAGGG+AGG	0.643397	7.2:-47664323	MS.gene022932:CDS
GTTCTCCGTGATAACATCCA+GGG	0.644292	7.2:-47664260	MS.gene022932:CDS
AAGCGCATAGACAACATCCA+TGG	0.648977	7.2:+47664073	None:intergenic
TGAGCACGCAAGGAGGAAGA+CGG	0.652638	7.2:-47664102	MS.gene022932:CDS
GGTGGTGTTAAGAGAATCAG+CGG	0.661394	7.2:-47664200	MS.gene022932:CDS
GGTAAGGGACTTGGAAAAGG+AGG	0.661416	7.2:-47664302	MS.gene022932:CDS
CAGTGTACGTAACAGCATCA+CGG	0.661438	7.2:+47664123	None:intergenic
TTGATCTATGAAGAAACCAG+AGG	0.713224	7.2:-47664176	MS.gene022932:CDS
CGTACACTGAGCACGCAAGG+AGG	0.720932	7.2:-47664109	MS.gene022932:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGATCTATGAAGAAACCAG+AGG	-	chr7.2:47664181-47664200	MS.gene022932:CDS	35.0%
	AGGTGTCTTGAAGATCTTCT+TGG	-	chr7.2:47664201-47664220	MS.gene022932:CDS	40.0%
	ATGTTGTCTATGCGCTTAAG+AGG	-	chr7.2:47664290-47664309	MS.gene022932:CDS	40.0%
	TGATACCCTGGATGTTATCA+CGG	+	chr7.2:47664105-47664124	None:intergenic	40.0%
	AAGCGCATAGACAACATCCA+TGG	+	chr7.2:47664287-47664306	None:intergenic	45.0%
	AGAACCCTCTATGGATTTGG+CGG	-	chr7.2:47664319-47664338	MS.gene022932:CDS	45.0%
	AGTTCTCCGTGATAACATCC+AGG	-	chr7.2:47664096-47664115	MS.gene022932:CDS	45.0%
	CAGTGTACGTAACAGCATCA+CGG	+	chr7.2:47664237-47664256	None:intergenic	45.0%
	GAAGATCTTCAAGACACCTC+TGG	+	chr7.2:47664200-47664219	None:intergenic	45.0%
	GGAAGAACCCTCTATGGATT+TGG	-	chr7.2:47664316-47664335	MS.gene022932:CDS	45.0%
	GTTCTCCGTGATAACATCCA+GGG	-	chr7.2:47664097-47664116	MS.gene022932:CDS	45.0%
	TCAACCGCCAAATCCATAGA+GGG	+	chr7.2:47664326-47664345	None:intergenic	45.0%
!	AAGGAGGTGCAAAGAGACAT+AGG	-	chr7.2:47664071-47664090	MS.gene022932:CDS	45.0%
!	ATTCGTCGATTGGCAAGAAG+AGG	-	chr7.2:47664136-47664155	MS.gene022932:CDS	45.0%
!	GGTGGTGTTAAGAGAATCAG+CGG	-	chr7.2:47664157-47664176	MS.gene022932:CDS	45.0%
	AGGCAAGGAAGAACCCTCTA+TGG	-	chr7.2:47664310-47664329	MS.gene022932:CDS	50.0%
	GGAGGTAAGGGACTTGGAAA+AGG	-	chr7.2:47664052-47664071	MS.gene022932:CDS	50.0%
	GGTAAGGGACTTGGAAAAGG+AGG	-	chr7.2:47664055-47664074	MS.gene022932:CDS	50.0%
	GTCTATGCGCTTAAGAGGCA+AGG	-	chr7.2:47664295-47664314	MS.gene022932:CDS	50.0%
	TTACGTACACTGAGCACGCA+AGG	-	chr7.2:47664245-47664264	MS.gene022932:CDS	50.0%
	AGGTCGTGGAAAGGGAGGTA+AGG	-	chr7.2:47664039-47664058	MS.gene022932:CDS	55.0%
	ATGTCAGGTCGTGGAAAGGG+AGG	-	chr7.2:47664034-47664053	MS.gene022932:CDS	55.0%
	CAAGCCTGCGATTCGTCGAT+TGG	-	chr7.2:47664126-47664145	MS.gene022932:CDS	55.0%
	CAATCGACGAATCGCAGGCT+TGG	+	chr7.2:47664128-47664147	None:intergenic	55.0%
	CTTGCCAATCGACGAATCGC+AGG	+	chr7.2:47664133-47664152	None:intergenic	55.0%
	GGAAAGGGAGGTAAGGGACT+TGG	-	chr7.2:47664046-47664065	MS.gene022932:CDS	55.0%
	GGTCGTGGAAAGGGAGGTAA+GGG	-	chr7.2:47664040-47664059	MS.gene022932:CDS	55.0%
	TGAGCACGCAAGGAGGAAGA+CGG	-	chr7.2:47664255-47664274	MS.gene022932:CDS	55.0%
!	CGTCGATTGGCAAGAAGAGG+TGG	-	chr7.2:47664139-47664158	MS.gene022932:CDS	55.0%
	CGTACACTGAGCACGCAAGG+AGG	-	chr7.2:47664248-47664267	MS.gene022932:CDS	60.0%
	GAGGAAGACGGTGACAGCCA+TGG	-	chr7.2:47664267-47664286	MS.gene022932:CDS	60.0%
!!	TCGCAGGCTTGGTGATACCC+TGG	+	chr7.2:47664117-47664136	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	47664034	47664345	47664034	ID=MS.gene022932
chr7.2	mRNA	47664034	47664345	47664034	ID=MS.gene022932.t1;Parent=MS.gene022932
chr7.2	exon	47664034	47664345	47664034	ID=MS.gene022932.t1.exon1;Parent=MS.gene022932.t1
chr7.2	CDS	47664034	47664345	47664034	ID=cds.MS.gene022932.t1;Parent=MS.gene022932.t1

Gene Sequence	>MS.gene022932 ATGTCAGGTCGTGGAAAGGGAGGTAAGGGACTTGGAAAAGGAGGTGCAAAGAGACATAGGAAAGTTCTCCGTGATAACATCCAGGGTATCACCAAGCCTGCGATTCGTCGATTGGCAAGAAGAGGTGGTGTTAAGAGAATCAGCGGTTTGATCTATGAAGAAACCAGAGGTGTCTTGAAGATCTTCTTGGAAAATGTGATCCGTGATGCTGTTACGTACACTGAGCACGCAAGGAGGAAGACGGTGACAGCCATGGATGTTGTCTATGCGCTTAAGAGGCAAGGAAGAACCCTCTATGGATTTGGCGGTTGA
Protein sequence	>MS.gene022932.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG