AlfalfaGEDB | MS.gene04052

M. sativa cultivar XinJiangDaYe / MS.gene04052

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04052.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04052.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04052.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene061876	MS.gene04052	PPI
MS.gene05608	MS.gene04052	PPI
MS.gene012391	MS.gene04052	PPI
MS.gene070244	MS.gene04052	PPI
MS.gene09871	MS.gene04052	PPI
MS.gene035897	MS.gene04052	PPI
MS.gene64920	MS.gene04052	PPI
MS.gene060463	MS.gene04052	PPI
MS.gene31196	MS.gene04052	PPI
MS.gene010219	MS.gene04052	PPI
MS.gene04052	MS.gene84536	PPI
MS.gene060465	MS.gene04052	PPI
MS.gene04068	MS.gene04052	PPI
MS.gene04052	MS.gene60093	PPI
MS.gene04052	MS.gene09873	PPI
MS.gene04434	MS.gene04052	PPI
MS.gene08121	MS.gene04052	PPI
MS.gene04052	MS.gene04972	PPI
MS.gene04052	MS.gene004121	PPI
MS.gene022932	MS.gene04052	PPI
MS.gene04052	MS.gene62520	PPI
MS.gene88681	MS.gene04052	PPI
MS.gene04052	MS.gene018578	PPI
MS.gene035718	MS.gene04052	PPI
MS.gene035896	MS.gene04052	PPI
MS.gene73136	MS.gene04052	PPI
MS.gene008276	MS.gene04052	PPI
MS.gene04052	MS.gene018580	PPI
MS.gene04052	MS.gene004124	PPI
MS.gene070907	MS.gene04052	PPI
MS.gene009027	MS.gene04052	PPI
MS.gene09873	MS.gene04052	PPI
MS.gene78578	MS.gene04052	PPI
MS.gene08122	MS.gene04052	PPI
MS.gene04052	MS.gene061866	PPI
MS.gene048705	MS.gene04052	PPI
MS.gene05623	MS.gene04052	PPI
MS.gene043967	MS.gene04052	PPI
MS.gene04052	MS.gene64650	PPI
MS.gene04052	MS.gene048705	PPI
MS.gene84536	MS.gene04052	PPI
MS.gene028629	MS.gene04052	PPI
MS.gene04052	MS.gene061874	PPI
MS.gene04052	MS.gene04576	PPI
MS.gene82546	MS.gene04052	PPI
MS.gene04433	MS.gene04052	PPI
MS.gene04052	MS.gene061869	PPI
MS.gene04067	MS.gene04052	PPI
MS.gene71215	MS.gene04052	PPI
MS.gene061866	MS.gene04052	PPI
MS.gene061870	MS.gene04052	PPI
MS.gene04052	MS.gene061867	PPI
MS.gene66225	MS.gene04052	PPI
MS.gene04052	MS.gene008276	PPI
MS.gene071136	MS.gene04052	PPI
MS.gene040383	MS.gene04052	PPI
MS.gene070242	MS.gene04052	PPI
MS.gene012390	MS.gene04052	PPI
MS.gene04052	MS.gene05619	PPI
MS.gene04052	MS.gene73136	PPI
MS.gene79195	MS.gene04052	PPI
MS.gene035721	MS.gene04052	PPI
MS.gene04052	MS.gene89488	PPI
MS.gene056215	MS.gene04052	PPI
MS.gene064557	MS.gene04052	PPI
MS.gene038469	MS.gene04052	PPI
MS.gene04052	MS.gene035720	PPI
MS.gene04052	MS.gene61017	PPI
MS.gene61017	MS.gene04052	PPI
MS.gene04452	MS.gene04052	PPI
MS.gene04052	MS.gene012390	PPI
MS.gene04052	MS.gene049705	PPI
MS.gene035787	MS.gene04052	PPI
MS.gene04576	MS.gene04052	PPI
MS.gene89487	MS.gene04052	PPI
MS.gene04052	MS.gene010219	PPI
MS.gene04052	MS.gene05608	PPI
MS.gene035719	MS.gene04052	PPI
MS.gene04052	MS.gene68545	PPI
MS.gene58854	MS.gene04052	PPI
MS.gene04052	MS.gene016624	PPI
MS.gene70955	MS.gene04052	PPI
MS.gene04392	MS.gene04052	PPI
MS.gene072493	MS.gene04052	PPI
MS.gene035720	MS.gene04052	PPI
MS.gene30920	MS.gene04052	PPI
MS.gene048746	MS.gene04052	PPI
MS.gene010220	MS.gene04052	PPI
MS.gene08053	MS.gene04052	PPI
MS.gene061872	MS.gene04052	PPI
MS.gene88680	MS.gene04052	PPI
MS.gene070241	MS.gene04052	PPI
MS.gene049821	MS.gene04052	PPI
MS.gene022946	MS.gene04052	PPI
MS.gene62520	MS.gene04052	PPI
MS.gene04064	MS.gene04052	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04052.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04052.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04052.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04052.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04052.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04052.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04052.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04052.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04052.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04052.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04052.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04052.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04052.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04052.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04052.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene04052.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04052.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04052.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04052.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04052.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04052.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04052.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04052.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04052.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04052.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04052.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04052.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 31 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGTGTCTTGAAGATCTTCT+TGG	0.405705	3.3:+50337198	MS.gene04052:CDS
AGACAGGGAAGAACGCTCTA+TGG	0.434419	3.3:+50337307	MS.gene04052:CDS
GGAAGAACGCTCTATGGTTT+CGG	0.438131	3.3:+50337313	MS.gene04052:CDS
AAAATGTCAGGTCGTGGAAA+GGG	0.446785	3.3:+50337028	None:intergenic
TGTCTATGCTTTGAAGAGAC+AGG	0.458633	3.3:+50337291	MS.gene04052:CDS
GGTAAGGGACTCGGAAAGGG+AGG	0.466154	3.3:+50337052	MS.gene04052:CDS
CTTGCCAATCGACGAATAGC+AGG	0.484591	3.3:-50337127	None:intergenic
CTGTTGAAAATGTCAGGTCG+TGG	0.487154	3.3:+50337022	None:intergenic
GAAGATCTTCAAGACACCTC+TGG	0.510902	3.3:-50337194	None:intergenic
AGTTCTTCGTGATAACATCC+AGG	0.522451	3.3:+50337093	MS.gene04052:CDS
CAATCGACGAATAGCAGGCT+TGG	0.531595	3.3:-50337122	None:intergenic
CAAGCCTGCTATTCGTCGAT+TGG	0.532828	3.3:+50337123	MS.gene04052:CDS
GGTGGTGTTAAGAGAATCAG+TGG	0.556350	3.3:+50337154	MS.gene04052:CDS
AAATGTCAGGTCGTGGAAAG+GGG	0.560416	3.3:+50337029	None:intergenic
GAGGAAGACGGTGACAGCCA+TGG	0.561434	3.3:+50337264	MS.gene04052:CDS
AGGGAGGTGCAAAGAGACAT+AGG	0.568952	3.3:+50337068	MS.gene04052:CDS
AATGTCAGGTCGTGGAAAGG+GGG	0.581791	3.3:+50337030	None:intergenic
GTCTATGCTTTGAAGAGACA+GGG	0.584038	3.3:+50337292	MS.gene04052:CDS
GAAAATGTCAGGTCGTGGAA+AGG	0.584382	3.3:+50337027	None:intergenic
CGTCGATTGGCAAGAAGAGG+TGG	0.593946	3.3:+50337136	MS.gene04052:CDS
TAGCAGGCTTGGTGATACCC+TGG	0.594313	3.3:-50337111	None:intergenic
TTACATACACTGAGCACGCA+AGG	0.596203	3.3:+50337242	MS.gene04052:CDS
ATGTCAGGTCGTGGAAAGGG+GGG	0.615792	3.3:+50337031	MS.gene04052:CDS
AGAACGCTCTATGGTTTCGG+AGG	0.628880	3.3:+50337316	MS.gene04052:CDS
ATTCGTCGATTGGCAAGAAG+AGG	0.629429	3.3:+50337133	MS.gene04052:CDS
CAAAGCATAGACAACATCCA+TGG	0.629842	3.3:-50337281	None:intergenic
GTTCTTCGTGATAACATCCA+GGG	0.641996	3.3:+50337094	MS.gene04052:CDS
TGAGCACGCAAGGAGGAAGA+CGG	0.652638	3.3:+50337252	MS.gene04052:CDS
CAGTGTATGTAACTGCATCA+CGG	0.689582	3.3:-50337231	None:intergenic
TTGATCTATGAAGAAACCAG+AGG	0.704889	3.3:+50337178	MS.gene04052:CDS
CATACACTGAGCACGCAAGG+AGG	0.730442	3.3:+50337245	MS.gene04052:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGATCTATGAAGAAACCAG+AGG	+	chr3.3:50337178-50337197	MS.gene04052:CDS	35.0%
	AGGTGTCTTGAAGATCTTCT+TGG	+	chr3.3:50337198-50337217	MS.gene04052:CDS	40.0%
	AGTTCTTCGTGATAACATCC+AGG	+	chr3.3:50337093-50337112	MS.gene04052:CDS	40.0%
	CAAAGCATAGACAACATCCA+TGG	-	chr3.3:50337284-50337303	None:intergenic	40.0%
	CAGTGTATGTAACTGCATCA+CGG	-	chr3.3:50337234-50337253	None:intergenic	40.0%
	GTTCTTCGTGATAACATCCA+GGG	+	chr3.3:50337094-50337113	MS.gene04052:CDS	40.0%
!!	GTCTATGCTTTGAAGAGACA+GGG	+	chr3.3:50337292-50337311	MS.gene04052:CDS	40.0%
!!	TGTCTATGCTTTGAAGAGAC+AGG	+	chr3.3:50337291-50337310	MS.gene04052:CDS	40.0%
	GAAGATCTTCAAGACACCTC+TGG	-	chr3.3:50337197-50337216	None:intergenic	45.0%
	GGAAGAACGCTCTATGGTTT+CGG	+	chr3.3:50337313-50337332	MS.gene04052:CDS	45.0%
	TTACATACACTGAGCACGCA+AGG	+	chr3.3:50337242-50337261	MS.gene04052:CDS	45.0%
!	ATTCGTCGATTGGCAAGAAG+AGG	+	chr3.3:50337133-50337152	MS.gene04052:CDS	45.0%
!	GGTGGTGTTAAGAGAATCAG+TGG	+	chr3.3:50337154-50337173	MS.gene04052:CDS	45.0%
	AGAACGCTCTATGGTTTCGG+AGG	+	chr3.3:50337316-50337335	MS.gene04052:CDS	50.0%
	AGACAGGGAAGAACGCTCTA+TGG	+	chr3.3:50337307-50337326	MS.gene04052:CDS	50.0%
	CAAGCCTGCTATTCGTCGAT+TGG	+	chr3.3:50337123-50337142	MS.gene04052:CDS	50.0%
	CAATCGACGAATAGCAGGCT+TGG	-	chr3.3:50337125-50337144	None:intergenic	50.0%
	CTTGCCAATCGACGAATAGC+AGG	-	chr3.3:50337130-50337149	None:intergenic	50.0%
!	AGGGAGGTGCAAAGAGACAT+AGG	+	chr3.3:50337068-50337087	MS.gene04052:CDS	50.0%
	ATGTCAGGTCGTGGAAAGGG+GGG	+	chr3.3:50337031-50337050	MS.gene04052:CDS	55.0%
	CATACACTGAGCACGCAAGG+AGG	+	chr3.3:50337245-50337264	MS.gene04052:CDS	55.0%
	TGAGCACGCAAGGAGGAAGA+CGG	+	chr3.3:50337252-50337271	MS.gene04052:CDS	55.0%
!	CGTCGATTGGCAAGAAGAGG+TGG	+	chr3.3:50337136-50337155	MS.gene04052:CDS	55.0%
!!	TAGCAGGCTTGGTGATACCC+TGG	-	chr3.3:50337114-50337133	None:intergenic	55.0%
	AGGTCGTGGAAAGGGGGGTA+AGG	+	chr3.3:50337036-50337055	MS.gene04052:CDS	60.0%
	GAGGAAGACGGTGACAGCCA+TGG	+	chr3.3:50337264-50337283	MS.gene04052:CDS	60.0%
	GGAAAGGGGGGTAAGGGACT+CGG	+	chr3.3:50337043-50337062	MS.gene04052:CDS	60.0%
	GGGGGTAAGGGACTCGGAAA+GGG	+	chr3.3:50337049-50337068	MS.gene04052:CDS	60.0%
	GGTAAGGGACTCGGAAAGGG+AGG	+	chr3.3:50337052-50337071	MS.gene04052:CDS	60.0%
	GGTCGTGGAAAGGGGGGTAA+GGG	+	chr3.3:50337037-50337056	MS.gene04052:CDS	60.0%
	GGGGGGTAAGGGACTCGGAA+AGG	+	chr3.3:50337048-50337067	MS.gene04052:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	50337031	50337342	50337031	ID=MS.gene04052
chr3.3	mRNA	50337031	50337342	50337031	ID=MS.gene04052.t1;Parent=MS.gene04052
chr3.3	exon	50337031	50337342	50337031	ID=MS.gene04052.t1.exon1;Parent=MS.gene04052.t1
chr3.3	CDS	50337031	50337342	50337031	ID=cds.MS.gene04052.t1;Parent=MS.gene04052.t1

Gene Sequence	>MS.gene04052 ATGTCAGGTCGTGGAAAGGGGGGTAAGGGACTCGGAAAGGGAGGTGCAAAGAGACATAGGAAAGTTCTTCGTGATAACATCCAGGGTATCACCAAGCCTGCTATTCGTCGATTGGCAAGAAGAGGTGGTGTTAAGAGAATCAGTGGATTGATCTATGAAGAAACCAGAGGTGTCTTGAAGATCTTCTTGGAAAATGTCATCCGTGATGCAGTTACATACACTGAGCACGCAAGGAGGAAGACGGTGACAGCCATGGATGTTGTCTATGCTTTGAAGAGACAGGGAAGAACGCTCTATGGTTTCGGAGGTTGA
Protein sequence	>MS.gene04052.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG