AlfalfaGEDB | MS.gene05619

M. sativa cultivar XinJiangDaYe / MS.gene05619

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05619.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05619.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05619.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene09871	MS.gene05619	PPI
MS.gene04576	MS.gene05619	PPI
MS.gene89487	MS.gene05619	PPI
MS.gene05619	MS.gene64650	PPI
MS.gene05619	MS.gene061874	PPI
MS.gene05619	MS.gene061869	PPI
MS.gene04392	MS.gene05619	PPI
MS.gene30920	MS.gene05619	PPI
MS.gene08053	MS.gene05619	PPI
MS.gene061872	MS.gene05619	PPI
MS.gene04068	MS.gene05619	PPI
MS.gene070241	MS.gene05619	PPI
MS.gene04434	MS.gene05619	PPI
MS.gene022946	MS.gene05619	PPI
MS.gene05619	MS.gene061867	PPI
MS.gene04064	MS.gene05619	PPI
MS.gene061876	MS.gene05619	PPI
MS.gene05608	MS.gene05619	PPI
MS.gene022932	MS.gene05619	PPI
MS.gene012391	MS.gene05619	PPI
MS.gene88681	MS.gene05619	PPI
MS.gene070244	MS.gene05619	PPI
MS.gene035897	MS.gene05619	PPI
MS.gene035896	MS.gene05619	PPI
MS.gene64920	MS.gene05619	PPI
MS.gene05619	MS.gene89488	PPI
MS.gene060463	MS.gene05619	PPI
MS.gene070907	MS.gene05619	PPI
MS.gene009027	MS.gene05619	PPI
MS.gene31196	MS.gene05619	PPI
MS.gene010219	MS.gene05619	PPI
MS.gene060465	MS.gene05619	PPI
MS.gene05619	MS.gene049705	PPI
MS.gene061867	MS.gene05619	PPI
MS.gene05619	MS.gene68545	PPI
MS.gene05619	MS.gene016624	PPI
MS.gene08121	MS.gene05619	PPI
MS.gene061870	MS.gene05619	PPI
MS.gene035718	MS.gene05619	PPI
MS.gene73136	MS.gene05619	PPI
MS.gene008276	MS.gene05619	PPI
MS.gene09873	MS.gene05619	PPI
MS.gene78578	MS.gene05619	PPI
MS.gene035721	MS.gene05619	PPI
MS.gene04052	MS.gene05619	PPI
MS.gene08122	MS.gene05619	PPI
MS.gene048705	MS.gene05619	PPI
MS.gene05623	MS.gene05619	PPI
MS.gene043967	MS.gene05619	PPI
MS.gene064557	MS.gene05619	PPI
MS.gene84536	MS.gene05619	PPI
MS.gene028629	MS.gene05619	PPI
MS.gene82546	MS.gene05619	PPI
MS.gene61017	MS.gene05619	PPI
MS.gene04433	MS.gene05619	PPI
MS.gene035787	MS.gene05619	PPI
MS.gene04067	MS.gene05619	PPI
MS.gene71215	MS.gene05619	PPI
MS.gene061866	MS.gene05619	PPI
MS.gene035719	MS.gene05619	PPI
MS.gene05619	MS.gene60093	PPI
MS.gene58854	MS.gene05619	PPI
MS.gene66225	MS.gene05619	PPI
MS.gene70955	MS.gene05619	PPI
MS.gene071136	MS.gene05619	PPI
MS.gene040383	MS.gene05619	PPI
MS.gene070242	MS.gene05619	PPI
MS.gene072493	MS.gene05619	PPI
MS.gene035720	MS.gene05619	PPI
MS.gene012390	MS.gene05619	PPI
MS.gene048746	MS.gene05619	PPI
MS.gene010220	MS.gene05619	PPI
MS.gene05619	MS.gene04972	PPI
MS.gene79195	MS.gene05619	PPI
MS.gene05619	MS.gene004121	PPI
MS.gene88680	MS.gene05619	PPI
MS.gene05619	MS.gene018578	PPI
MS.gene056215	MS.gene05619	PPI
MS.gene061869	MS.gene05619	PPI
MS.gene049821	MS.gene05619	PPI
MS.gene62520	MS.gene05619	PPI
MS.gene038469	MS.gene05619	PPI
MS.gene05619	MS.gene018580	PPI
MS.gene05619	MS.gene004124	PPI
MS.gene04452	MS.gene05619	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05619.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05619.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05619.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05619.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05619.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05619.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05619.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05619.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05619.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05619.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05619.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05619.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05619.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05619.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05619.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene05619.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05619.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05619.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05619.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05619.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05619.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05619.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05619.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05619.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05619.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05619.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05619.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 40 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTTCTTCCTTGTCTCTTCA+AGG	0.367893	3.2:-53487376	None:intergenic
CGTCTTCCTCCTTGCGTGCT+CGG	0.372219	3.2:-53487328	None:intergenic
AGACAAGGAAGAACCCTCTA+TGG	0.396310	3.2:+53487384	MS.gene05619:CDS
AGGTGTCTTGAAGATCTTCT+TGG	0.405705	3.2:+53487275	MS.gene05619:CDS
GGGAGGTAAGGGACTAGGAA+AGG	0.428277	3.2:+53487125	MS.gene05619:CDS
GGAGGTAAGGGACTAGGAAA+GGG	0.452874	3.2:+53487126	MS.gene05619:CDS
CTCTTGAAAATGTCAGGTCG+TGG	0.456977	3.2:+53487099	None:intergenic
TTCAACCTCCGAATCCATAG+AGG	0.479629	3.2:-53487398	None:intergenic
CTTGCCAATCGACGAATAGC+AGG	0.484591	3.2:-53487204	None:intergenic
GGAAGAACCCTCTATGGATT+CGG	0.491141	3.2:+53487390	MS.gene05619:CDS
AGGTCGTGGAAAGGGAGGTA+AGG	0.492819	3.2:+53487113	MS.gene05619:CDS
AGGGAGGTGCAAAGAGGCAT+AGG	0.498985	3.2:+53487145	MS.gene05619:CDS
GGTTCTTCGTGACAACATCC+AGG	0.501637	3.2:+53487170	MS.gene05619:CDS
CCTCCTTGCGTGCTCGGTGT+AGG	0.506474	3.2:-53487322	None:intergenic
GAAGATCTTCAAGACACCTC+TGG	0.510902	3.2:-53487271	None:intergenic
GGAAAGGGAGGTAAGGGACT+AGG	0.521051	3.2:+53487120	MS.gene05619:CDS
CAATCGACGAATAGCAGGCT+TGG	0.531595	3.2:-53487199	None:intergenic
CAAGCCTGCTATTCGTCGAT+TGG	0.532828	3.2:+53487200	MS.gene05619:CDS
AAAATGTCAGGTCGTGGAAA+GGG	0.538992	3.2:+53487105	None:intergenic
TGACCTACACCGAGCACGCA+AGG	0.543870	3.2:+53487319	MS.gene05619:CDS
GAGGAAGACGGTGACAGCCA+TGG	0.561434	3.2:+53487341	MS.gene05619:CDS
GGTGGTGTTAAGAGAATCAG+TGG	0.562326	3.2:+53487231	MS.gene05619:CDS
GGTAAGGGACTAGGAAAGGG+AGG	0.563878	3.2:+53487129	MS.gene05619:CDS
GGTCGTGGAAAGGGAGGTAA+GGG	0.564245	3.2:+53487114	MS.gene05619:CDS
GAAAATGTCAGGTCGTGGAA+AGG	0.584382	3.2:+53487104	None:intergenic
AGGTGCAAAGAGGCATAGGA+AGG	0.586086	3.2:+53487149	MS.gene05619:CDS
CGTCGATTGGCAAGAAGAGG+TGG	0.593946	3.2:+53487213	MS.gene05619:CDS
TAGCAGGCTTGGTGATACCC+TGG	0.594313	3.2:-53487188	None:intergenic
GTCTATGCCTTGAAGAGACA+AGG	0.608946	3.2:+53487369	MS.gene05619:CDS
CGAGCACGCAAGGAGGAAGA+CGG	0.619274	3.2:+53487329	MS.gene05619:CDS
ATTCGTCGATTGGCAAGAAG+AGG	0.629429	3.2:+53487210	MS.gene05619:CDS
CAAGGCATAGACAACATCCA+TGG	0.629842	3.2:-53487358	None:intergenic
CGGTGTAGGTCACAGCATCA+CGG	0.635051	3.2:-53487308	None:intergenic
ATGTCAGGTCGTGGAAAGGG+AGG	0.643397	3.2:+53487108	MS.gene05619:CDS
TCAACCTCCGAATCCATAGA+GGG	0.644865	3.2:-53487397	None:intergenic
AGAACCCTCTATGGATTCGG+AGG	0.656308	3.2:+53487393	MS.gene05619:CDS
GTTCTTCGTGACAACATCCA+GGG	0.676380	3.2:+53487171	MS.gene05619:CDS
TTGATATATGAAGAAACCAG+AGG	0.683025	3.2:+53487255	MS.gene05619:CDS
TAGGAAAGGGAGGTGCAAAG+AGG	0.683932	3.2:+53487139	MS.gene05619:CDS
CCTACACCGAGCACGCAAGG+AGG	0.730442	3.2:+53487322	MS.gene05619:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGATATATGAAGAAACCAG+AGG	+	chr3.2:53487255-53487274	MS.gene05619:CDS	30.0%
	AGGTGTCTTGAAGATCTTCT+TGG	+	chr3.2:53487275-53487294	MS.gene05619:CDS	40.0%
	AGACAAGGAAGAACCCTCTA+TGG	+	chr3.2:53487384-53487403	MS.gene05619:CDS	45.0%
	CAAGGCATAGACAACATCCA+TGG	-	chr3.2:53487361-53487380	None:intergenic	45.0%
	GAAGATCTTCAAGACACCTC+TGG	-	chr3.2:53487274-53487293	None:intergenic	45.0%
	GGAAGAACCCTCTATGGATT+CGG	+	chr3.2:53487390-53487409	MS.gene05619:CDS	45.0%
	GGTTCTTCCTTGTCTCTTCA+AGG	-	chr3.2:53487379-53487398	None:intergenic	45.0%
	GTCTATGCCTTGAAGAGACA+AGG	+	chr3.2:53487369-53487388	MS.gene05619:CDS	45.0%
	GTTCTTCGTGACAACATCCA+GGG	+	chr3.2:53487171-53487190	MS.gene05619:CDS	45.0%
	TCAACCTCCGAATCCATAGA+GGG	-	chr3.2:53487400-53487419	None:intergenic	45.0%
!	ATTCGTCGATTGGCAAGAAG+AGG	+	chr3.2:53487210-53487229	MS.gene05619:CDS	45.0%
!	GGTGGTGTTAAGAGAATCAG+TGG	+	chr3.2:53487231-53487250	MS.gene05619:CDS	45.0%
	AGAACCCTCTATGGATTCGG+AGG	+	chr3.2:53487393-53487412	MS.gene05619:CDS	50.0%
	CAAGCCTGCTATTCGTCGAT+TGG	+	chr3.2:53487200-53487219	MS.gene05619:CDS	50.0%
	CAATCGACGAATAGCAGGCT+TGG	-	chr3.2:53487202-53487221	None:intergenic	50.0%
	CTTGCCAATCGACGAATAGC+AGG	-	chr3.2:53487207-53487226	None:intergenic	50.0%
	GGAGGTAAGGGACTAGGAAA+GGG	+	chr3.2:53487126-53487145	MS.gene05619:CDS	50.0%
	GGTTCTTCGTGACAACATCC+AGG	+	chr3.2:53487170-53487189	MS.gene05619:CDS	50.0%
!	AGGTGCAAAGAGGCATAGGA+AGG	+	chr3.2:53487149-53487168	MS.gene05619:CDS	50.0%
!	TAGGAAAGGGAGGTGCAAAG+AGG	+	chr3.2:53487139-53487158	MS.gene05619:CDS	50.0%
	AGGTCGTGGAAAGGGAGGTA+AGG	+	chr3.2:53487113-53487132	MS.gene05619:CDS	55.0%
	ATGTCAGGTCGTGGAAAGGG+AGG	+	chr3.2:53487108-53487127	MS.gene05619:CDS	55.0%
	CGGTGTAGGTCACAGCATCA+CGG	-	chr3.2:53487311-53487330	None:intergenic	55.0%
	GGAAAGGGAGGTAAGGGACT+AGG	+	chr3.2:53487120-53487139	MS.gene05619:CDS	55.0%
	GGGAGGTAAGGGACTAGGAA+AGG	+	chr3.2:53487125-53487144	MS.gene05619:CDS	55.0%
	GGTAAGGGACTAGGAAAGGG+AGG	+	chr3.2:53487129-53487148	MS.gene05619:CDS	55.0%
	GGTCGTGGAAAGGGAGGTAA+GGG	+	chr3.2:53487114-53487133	MS.gene05619:CDS	55.0%
!	AGGGAGGTGCAAAGAGGCAT+AGG	+	chr3.2:53487145-53487164	MS.gene05619:CDS	55.0%
!	CGTCGATTGGCAAGAAGAGG+TGG	+	chr3.2:53487213-53487232	MS.gene05619:CDS	55.0%
!!	TAGCAGGCTTGGTGATACCC+TGG	-	chr3.2:53487191-53487210	None:intergenic	55.0%
	CGAGCACGCAAGGAGGAAGA+CGG	+	chr3.2:53487329-53487348	MS.gene05619:CDS	60.0%
	CGTCTTCCTCCTTGCGTGCT+CGG	-	chr3.2:53487331-53487350	None:intergenic	60.0%
	GAGGAAGACGGTGACAGCCA+TGG	+	chr3.2:53487341-53487360	MS.gene05619:CDS	60.0%
	TGACCTACACCGAGCACGCA+AGG	+	chr3.2:53487319-53487338	MS.gene05619:CDS	60.0%
	CCTACACCGAGCACGCAAGG+AGG	+	chr3.2:53487322-53487341	MS.gene05619:CDS	65.0%
!!	CCTCCTTGCGTGCTCGGTGT+AGG	-	chr3.2:53487325-53487344	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	53487108	53487419	53487108	ID=MS.gene05619
chr3.2	mRNA	53487108	53487419	53487108	ID=MS.gene05619.t1;Parent=MS.gene05619
chr3.2	exon	53487108	53487419	53487108	ID=MS.gene05619.t1.exon1;Parent=MS.gene05619.t1
chr3.2	CDS	53487108	53487419	53487108	ID=cds.MS.gene05619.t1;Parent=MS.gene05619.t1

Gene Sequence	>MS.gene05619 ATGTCAGGTCGTGGAAAGGGAGGTAAGGGACTAGGAAAGGGAGGTGCAAAGAGGCATAGGAAGGTTCTTCGTGACAACATCCAGGGTATCACCAAGCCTGCTATTCGTCGATTGGCAAGAAGAGGTGGTGTTAAGAGAATCAGTGGTTTGATATATGAAGAAACCAGAGGTGTCTTGAAGATCTTCTTGGAAAATGTCATCCGTGATGCTGTGACCTACACCGAGCACGCAAGGAGGAAGACGGTGACAGCCATGGATGTTGTCTATGCCTTGAAGAGACAAGGAAGAACCCTCTATGGATTCGGAGGTTGA
Protein sequence	>MS.gene05619.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG