AlfalfaGEDB | MS.gene04452

M. sativa cultivar XinJiangDaYe / MS.gene04452

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04452.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04452.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04452.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene70955	MS.gene04452	PPI
MS.gene04452	MS.gene04068	PPI
MS.gene040383	MS.gene04452	PPI
MS.gene04452	MS.gene022946	PPI
MS.gene04452	MS.gene05623	PPI
MS.gene30920	MS.gene04452	PPI
MS.gene04452	MS.gene89488	PPI
MS.gene08053	MS.gene04452	PPI
MS.gene061872	MS.gene04452	PPI
MS.gene04452	MS.gene66225	PPI
MS.gene04452	MS.gene035720	PPI
MS.gene04452	MS.gene70955	PPI
MS.gene04452	MS.gene61017	PPI
MS.gene88680	MS.gene04452	PPI
MS.gene04452	MS.gene09871	PPI
MS.gene070241	MS.gene04452	PPI
MS.gene056215	MS.gene04452	PPI
MS.gene04452	MS.gene012390	PPI
MS.gene04452	MS.gene08121	PPI
MS.gene04452	MS.gene064557	PPI
MS.gene04452	MS.gene049705	PPI
MS.gene04452	MS.gene04434	PPI
MS.gene04452	MS.gene070907	PPI
MS.gene061876	MS.gene04452	PPI
MS.gene04452	MS.gene04392	PPI
MS.gene012391	MS.gene04452	PPI
MS.gene04452	MS.gene010219	PPI
MS.gene04452	MS.gene05608	PPI
MS.gene04452	MS.gene040383	PPI
MS.gene04452	MS.gene68545	PPI
MS.gene060463	MS.gene04452	PPI
MS.gene04452	MS.gene070242	PPI
MS.gene04452	MS.gene016624	PPI
MS.gene04452	MS.gene048746	PPI
MS.gene04452	MS.gene09873	PPI
MS.gene04452	MS.gene043967	PPI
MS.gene04452	MS.gene035897	PPI
MS.gene04452	MS.gene035721	PPI
MS.gene08121	MS.gene04452	PPI
MS.gene88681	MS.gene04452	PPI
MS.gene070244	MS.gene04452	PPI
MS.gene64920	MS.gene04452	PPI
MS.gene04452	MS.gene072493	PPI
MS.gene04452	MS.gene84536	PPI
MS.gene04452	MS.gene035896	PPI
MS.gene009027	MS.gene04452	PPI
MS.gene04452	MS.gene82546	PPI
MS.gene31196	MS.gene04452	PPI
MS.gene04452	MS.gene60093	PPI
MS.gene060465	MS.gene04452	PPI
MS.gene04452	MS.gene08122	PPI
MS.gene04452	MS.gene022932	PPI
MS.gene028629	MS.gene04452	PPI
MS.gene04452	MS.gene010220	PPI
MS.gene04452	MS.gene04972	PPI
MS.gene04452	MS.gene004121	PPI
MS.gene04452	MS.gene62520	PPI
MS.gene04452	MS.gene018578	PPI
MS.gene71215	MS.gene04452	PPI
MS.gene061870	MS.gene04452	PPI
MS.gene04452	MS.gene04064	PPI
MS.gene04452	MS.gene018580	PPI
MS.gene04452	MS.gene004124	PPI
MS.gene035718	MS.gene04452	PPI
MS.gene071136	MS.gene04452	PPI
MS.gene04452	MS.gene04052	PPI
MS.gene04452	MS.gene061866	PPI
MS.gene04452	MS.gene64650	PPI
MS.gene78578	MS.gene04452	PPI
MS.gene04452	MS.gene79195	PPI
MS.gene04452	MS.gene048705	PPI
MS.gene04452	MS.gene061874	PPI
MS.gene04452	MS.gene04576	PPI
MS.gene04452	MS.gene061869	PPI
MS.gene064557	MS.gene04452	PPI
MS.gene038469	MS.gene04452	PPI
MS.gene04433	MS.gene04452	PPI
MS.gene035787	MS.gene04452	PPI
MS.gene04067	MS.gene04452	PPI
MS.gene04452	MS.gene061867	PPI
MS.gene89487	MS.gene04452	PPI
MS.gene04452	MS.gene008276	PPI
MS.gene035719	MS.gene04452	PPI
MS.gene04452	MS.gene05619	PPI
MS.gene04452	MS.gene73136	PPI
MS.gene58854	MS.gene04452	PPI
MS.gene04452	MS.gene049821	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04452.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04452.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04452.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04452.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04452.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04452.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04452.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04452.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04452.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04452.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04452.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04452.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04452.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04452.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04452.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene04452.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04452.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04452.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04452.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04452.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04452.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04452.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04452.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04452.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04452.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04452.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04452.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 37 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTTCTTCCTTGTCTCTTCA+AGG	0.367893	3.4:+58116420	None:intergenic
CGTCTTCCTCCTTGCGTGCT+CGG	0.372219	3.4:+58116468	None:intergenic
AGACAAGGAAGAACCCTCTA+TGG	0.396310	3.4:-58116412	MS.gene04452:CDS
AGGTGTGTTGAAGATCTTCT+TGG	0.432619	3.4:-58116521	MS.gene04452:CDS
CTTGCCAATCGACGAATGGC+AGG	0.473855	3.4:+58116592	None:intergenic
TTCAACCTCCGAATCCATAG+AGG	0.479629	3.4:+58116398	None:intergenic
CTGTTGAAAATGTCAGGTCG+TGG	0.487154	3.4:-58116697	None:intergenic
GGTAAGGGACTCGGAAAGGG+AGG	0.488695	3.4:-58116667	MS.gene04452:CDS
GGAAGAACCCTCTATGGATT+CGG	0.491141	3.4:-58116406	MS.gene04452:CDS
AGGTCGTGGAAAGGGAGGTA+AGG	0.492819	3.4:-58116683	MS.gene04452:CDS
GGAGGTAAGGGACTCGGAAA+GGG	0.499726	3.4:-58116670	MS.gene04452:CDS
AAAGCCTGCCATTCGTCGAT+TGG	0.502268	3.4:-58116596	MS.gene04452:CDS
GAAGATCTTCAACACACCTC+TGG	0.516600	3.4:+58116525	None:intergenic
GGGAGGTAAGGGACTCGGAA+AGG	0.518226	3.4:-58116671	MS.gene04452:CDS
AGTTCTTCGTGATAACATCC+AGG	0.522451	3.4:-58116626	MS.gene04452:CDS
GGAAAGGGAGGTAAGGGACT+CGG	0.535187	3.4:-58116676	MS.gene04452:CDS
AAAATGTCAGGTCGTGGAAA+GGG	0.548959	3.4:-58116691	MS.gene04452:CDS
GAGGAAGACGGTGACAGCCA+TGG	0.561434	3.4:-58116455	MS.gene04452:CDS
GGTGGTGTTAAGAGAATCAG+TGG	0.562326	3.4:-58116565	MS.gene04452:CDS
GGTCGTGGAAAGGGAGGTAA+GGG	0.564245	3.4:-58116682	MS.gene04452:CDS
TGACATACACCGAGCACGCA+AGG	0.566909	3.4:-58116477	MS.gene04452:CDS
AGGGAGGTGCAAAGAGACAT+AGG	0.568952	3.4:-58116651	MS.gene04452:CDS
GAAAATGTCAGGTCGTGGAA+AGG	0.584382	3.4:-58116692	None:intergenic
TCTTCTTGCCAATCGACGAA+TGG	0.586064	3.4:+58116588	None:intergenic
CGTCGATTGGCAAGAAGAGG+TGG	0.589209	3.4:-58116583	MS.gene04452:CDS
GTCTATGCCTTGAAGAGACA+AGG	0.608946	3.4:-58116427	MS.gene04452:CDS
TGGCAGGCTTTGTGATACCC+TGG	0.617511	3.4:+58116608	None:intergenic
CGAGCACGCAAGGAGGAAGA+CGG	0.619274	3.4:-58116467	MS.gene04452:CDS
ATTCGTCGATTGGCAAGAAG+AGG	0.624691	3.4:-58116586	MS.gene04452:CDS
CAAGGCATAGACAACATCCA+TGG	0.629842	3.4:+58116438	None:intergenic
CGGTGTATGTCACAGCATCA+CGG	0.631400	3.4:+58116488	None:intergenic
GTTCTTCGTGATAACATCCA+GGG	0.641996	3.4:-58116625	MS.gene04452:CDS
ATGTCAGGTCGTGGAAAGGG+AGG	0.643397	3.4:-58116688	MS.gene04452:CDS
TCAACCTCCGAATCCATAGA+GGG	0.644865	3.4:+58116399	None:intergenic
AGAACCCTCTATGGATTCGG+AGG	0.656308	3.4:-58116403	MS.gene04452:CDS
TTGATATATGAAGAAACCAG+AGG	0.683025	3.4:-58116541	MS.gene04452:CDS
CATACACCGAGCACGCAAGG+AGG	0.730442	3.4:-58116474	MS.gene04452:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGATATATGAAGAAACCAG+AGG	-	chr3.4:58116546-58116565	MS.gene04452:CDS	30.0%
	AGGTGTGTTGAAGATCTTCT+TGG	-	chr3.4:58116566-58116585	MS.gene04452:CDS	40.0%
	AGTTCTTCGTGATAACATCC+AGG	-	chr3.4:58116461-58116480	MS.gene04452:CDS	40.0%
	GTTCTTCGTGATAACATCCA+GGG	-	chr3.4:58116462-58116481	MS.gene04452:CDS	40.0%
	AGACAAGGAAGAACCCTCTA+TGG	-	chr3.4:58116675-58116694	MS.gene04452:CDS	45.0%
	CAAGGCATAGACAACATCCA+TGG	+	chr3.4:58116652-58116671	None:intergenic	45.0%
	GAAGATCTTCAACACACCTC+TGG	+	chr3.4:58116565-58116584	None:intergenic	45.0%
	GGAAGAACCCTCTATGGATT+CGG	-	chr3.4:58116681-58116700	MS.gene04452:CDS	45.0%
	GGTTCTTCCTTGTCTCTTCA+AGG	+	chr3.4:58116670-58116689	None:intergenic	45.0%
	GTCTATGCCTTGAAGAGACA+AGG	-	chr3.4:58116660-58116679	MS.gene04452:CDS	45.0%
	TCAACCTCCGAATCCATAGA+GGG	+	chr3.4:58116691-58116710	None:intergenic	45.0%
	TCTTCTTGCCAATCGACGAA+TGG	+	chr3.4:58116502-58116521	None:intergenic	45.0%
!	ATTCGTCGATTGGCAAGAAG+AGG	-	chr3.4:58116501-58116520	MS.gene04452:CDS	45.0%
!	GGTGGTGTTAAGAGAATCAG+TGG	-	chr3.4:58116522-58116541	MS.gene04452:CDS	45.0%
	AAAGCCTGCCATTCGTCGAT+TGG	-	chr3.4:58116491-58116510	MS.gene04452:CDS	50.0%
	AGAACCCTCTATGGATTCGG+AGG	-	chr3.4:58116684-58116703	MS.gene04452:CDS	50.0%
	CGGTGTATGTCACAGCATCA+CGG	+	chr3.4:58116602-58116621	None:intergenic	50.0%
!	AGGGAGGTGCAAAGAGACAT+AGG	-	chr3.4:58116436-58116455	MS.gene04452:CDS	50.0%
	AGGTCGTGGAAAGGGAGGTA+AGG	-	chr3.4:58116404-58116423	MS.gene04452:CDS	55.0%
	ATGTCAGGTCGTGGAAAGGG+AGG	-	chr3.4:58116399-58116418	MS.gene04452:CDS	55.0%
	CTTGCCAATCGACGAATGGC+AGG	+	chr3.4:58116498-58116517	None:intergenic	55.0%
	GGAAAGGGAGGTAAGGGACT+CGG	-	chr3.4:58116411-58116430	MS.gene04452:CDS	55.0%
	GGAGGTAAGGGACTCGGAAA+GGG	-	chr3.4:58116417-58116436	MS.gene04452:CDS	55.0%
	GGTCGTGGAAAGGGAGGTAA+GGG	-	chr3.4:58116405-58116424	MS.gene04452:CDS	55.0%
	TGACATACACCGAGCACGCA+AGG	-	chr3.4:58116610-58116629	MS.gene04452:CDS	55.0%
!	CGTCGATTGGCAAGAAGAGG+TGG	-	chr3.4:58116504-58116523	MS.gene04452:CDS	55.0%
!	TGGCAGGCTTTGTGATACCC+TGG	+	chr3.4:58116482-58116501	None:intergenic	55.0%
	CATACACCGAGCACGCAAGG+AGG	-	chr3.4:58116613-58116632	MS.gene04452:CDS	60.0%
	CGAGCACGCAAGGAGGAAGA+CGG	-	chr3.4:58116620-58116639	MS.gene04452:CDS	60.0%
	CGTCTTCCTCCTTGCGTGCT+CGG	+	chr3.4:58116622-58116641	None:intergenic	60.0%
	GAGGAAGACGGTGACAGCCA+TGG	-	chr3.4:58116632-58116651	MS.gene04452:CDS	60.0%
	GGGAGGTAAGGGACTCGGAA+AGG	-	chr3.4:58116416-58116435	MS.gene04452:CDS	60.0%
	GGTAAGGGACTCGGAAAGGG+AGG	-	chr3.4:58116420-58116439	MS.gene04452:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	58116399	58116710	58116399	ID=MS.gene04452
chr3.4	mRNA	58116399	58116710	58116399	ID=MS.gene04452.t1;Parent=MS.gene04452
chr3.4	exon	58116399	58116710	58116399	ID=MS.gene04452.t1.exon1;Parent=MS.gene04452.t1
chr3.4	CDS	58116399	58116710	58116399	ID=cds.MS.gene04452.t1;Parent=MS.gene04452.t1

Gene Sequence	>MS.gene04452 ATGTCAGGTCGTGGAAAGGGAGGTAAGGGACTCGGAAAGGGAGGTGCAAAGAGACATAGGAAAGTTCTTCGTGATAACATCCAGGGTATCACAAAGCCTGCCATTCGTCGATTGGCAAGAAGAGGTGGTGTTAAGAGAATCAGTGGTTTGATATATGAAGAAACCAGAGGTGTGTTGAAGATCTTCTTGGAAAATGTCATCCGTGATGCTGTGACATACACCGAGCACGCAAGGAGGAAGACGGTGACAGCCATGGATGTTGTCTATGCCTTGAAGAGACAAGGAAGAACCCTCTATGGATTCGGAGGTTGA
Protein sequence	>MS.gene04452.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG