AlfalfaGEDB | MS.gene08122

M. sativa cultivar XinJiangDaYe / MS.gene08122

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08122.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08122.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08122.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene08122	MS.gene049705	PPI
MS.gene08121	MS.gene08122	PPI
MS.gene022932	MS.gene08122	PPI
MS.gene08122	MS.gene05608	PPI
MS.gene035718	MS.gene08122	PPI
MS.gene035896	MS.gene08122	PPI
MS.gene070907	MS.gene08122	PPI
MS.gene08122	MS.gene82546	PPI
MS.gene009027	MS.gene08122	PPI
MS.gene78578	MS.gene08122	PPI
MS.gene08122	MS.gene60093	PPI
MS.gene08122	MS.gene09873	PPI
MS.gene05623	MS.gene08122	PPI
MS.gene043967	MS.gene08122	PPI
MS.gene028629	MS.gene08122	PPI
MS.gene82546	MS.gene08122	PPI
MS.gene08122	MS.gene04972	PPI
MS.gene04433	MS.gene08122	PPI
MS.gene08122	MS.gene62520	PPI
MS.gene08122	MS.gene018578	PPI
MS.gene04067	MS.gene08122	PPI
MS.gene71215	MS.gene08122	PPI
MS.gene061870	MS.gene08122	PPI
MS.gene08122	MS.gene04064	PPI
MS.gene08122	MS.gene018580	PPI
MS.gene08122	MS.gene004124	PPI
MS.gene66225	MS.gene08122	PPI
MS.gene70955	MS.gene08122	PPI
MS.gene08122	MS.gene04052	PPI
MS.gene071136	MS.gene08122	PPI
MS.gene08122	MS.gene84536	PPI
MS.gene040383	MS.gene08122	PPI
MS.gene070242	MS.gene08122	PPI
MS.gene08122	MS.gene061866	PPI
MS.gene08122	MS.gene64650	PPI
MS.gene08122	MS.gene048705	PPI
MS.gene79195	MS.gene08122	PPI
MS.gene035721	MS.gene08122	PPI
MS.gene08122	MS.gene061874	PPI
MS.gene08122	MS.gene061869	PPI
MS.gene056215	MS.gene08122	PPI
MS.gene064557	MS.gene08122	PPI
MS.gene038469	MS.gene08122	PPI
MS.gene04452	MS.gene08122	PPI
MS.gene08122	MS.gene004121	PPI
MS.gene035787	MS.gene08122	PPI
MS.gene08122	MS.gene061867	PPI
MS.gene08122	MS.gene008276	PPI
MS.gene89487	MS.gene08122	PPI
MS.gene035719	MS.gene08122	PPI
MS.gene08122	MS.gene05619	PPI
MS.gene08122	MS.gene73136	PPI
MS.gene58854	MS.gene08122	PPI
MS.gene08122	MS.gene049821	PPI
MS.gene08122	MS.gene89488	PPI
MS.gene04392	MS.gene08122	PPI
MS.gene072493	MS.gene08122	PPI
MS.gene30920	MS.gene08122	PPI
MS.gene048746	MS.gene08122	PPI
MS.gene010220	MS.gene08122	PPI
MS.gene08053	MS.gene08122	PPI
MS.gene061872	MS.gene08122	PPI
MS.gene08122	MS.gene035720	PPI
MS.gene08122	MS.gene61017	PPI
MS.gene08122	MS.gene04576	PPI
MS.gene88680	MS.gene08122	PPI
MS.gene070241	MS.gene08122	PPI
MS.gene04434	MS.gene08122	PPI
MS.gene08122	MS.gene012390	PPI
MS.gene049821	MS.gene08122	PPI
MS.gene022946	MS.gene08122	PPI
MS.gene04064	MS.gene08122	PPI
MS.gene08122	MS.gene070907	PPI
MS.gene061876	MS.gene08122	PPI
MS.gene08122	MS.gene010219	PPI
MS.gene012391	MS.gene08122	PPI
MS.gene88681	MS.gene08122	PPI
MS.gene070244	MS.gene08122	PPI
MS.gene09871	MS.gene08122	PPI
MS.gene08122	MS.gene68545	PPI
MS.gene035897	MS.gene08122	PPI
MS.gene64920	MS.gene08122	PPI
MS.gene060463	MS.gene08122	PPI
MS.gene08122	MS.gene016624	PPI
MS.gene31196	MS.gene08122	PPI
MS.gene060465	MS.gene08122	PPI
MS.gene04068	MS.gene08122	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08122.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08122.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08122.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08122.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08122.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08122.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08122.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08122.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08122.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08122.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08122.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08122.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08122.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08122.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08122.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene08122.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08122.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08122.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08122.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08122.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08122.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08122.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08122.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08122.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08122.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08122.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08122.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 32 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGTGTATTGAAGATATTCT+TGG	0.398568	3.3:-50022330	MS.gene08122:CDS
AAAATGTCAGGTCGTGGAAA+GGG	0.434071	3.3:-50022500	MS.gene08122:CDS
AGACAGGGAAGAACGCTCTA+TGG	0.434419	3.3:-50022221	MS.gene08122:CDS
GGAAGAACGCTCTATGGTTT+CGG	0.438131	3.3:-50022215	MS.gene08122:CDS
CTCGCCAATCGGCGAATAGC+AGG	0.453484	3.3:+50022401	None:intergenic
TGTCTATGCTTTGAAGAGAC+AGG	0.458633	3.3:-50022237	MS.gene08122:CDS
GGTAAGGGTCTGGGAAAGGG+AGG	0.476615	3.3:-50022476	MS.gene08122:CDS
GAATATCTTCAATACACCTC+TGG	0.487673	3.3:+50022334	None:intergenic
CACCACCTCTTCTCGCCAAT+CGG	0.499060	3.3:+50022390	None:intergenic
GGTTCTTCGTGACAACATCC+AGG	0.501637	3.3:-50022435	MS.gene08122:CDS
CAAGCCTGCTATTCGCCGAT+TGG	0.502152	3.3:-50022405	MS.gene08122:CDS
CTCCCGAAAATGTCAGGTCG+TGG	0.510898	3.3:-50022506	None:intergenic
CAATCGGCGAATAGCAGGCT+TGG	0.515998	3.3:+50022406	None:intergenic
AGGGAGGTGCAAAGAGACAT+AGG	0.520046	3.3:-50022460	MS.gene08122:CDS
CGCCGATTGGCGAGAAGAGG+TGG	0.530277	3.3:-50022392	MS.gene08122:CDS
AAATGTCAGGTCGTGGAAAG+GGG	0.537019	3.3:-50022499	MS.gene08122:CDS
GGTGGTGTGAAGAGAATCAG+TGG	0.556654	3.3:-50022374	MS.gene08122:CDS
GAGGAAGACGGTGACAGCCA+TGG	0.561434	3.3:-50022264	MS.gene08122:CDS
GTCTATGCTTTGAAGAGACA+GGG	0.584038	3.3:-50022236	MS.gene08122:CDS
AATGTCAGGTCGTGGAAAGG+GGG	0.584231	3.3:-50022498	MS.gene08122:CDS
TGACATACACTGAGCACGCA+AGG	0.585419	3.3:-50022286	MS.gene08122:CDS
GAAAATGTCAGGTCGTGGAA+AGG	0.589881	3.3:-50022501	None:intergenic
TAGCAGGCTTGGTGATACCC+TGG	0.594313	3.3:+50022417	None:intergenic
ATTCGCCGATTGGCGAGAAG+AGG	0.615284	3.3:-50022395	MS.gene08122:CDS
ATGTCAGGTCGTGGAAAGGG+GGG	0.615792	3.3:-50022497	MS.gene08122:CDS
AGGTGCAAAGAGACATAGGA+AGG	0.617656	3.3:-50022456	MS.gene08122:CDS
AGAACGCTCTATGGTTTCGG+AGG	0.628880	3.3:-50022212	MS.gene08122:CDS
CAAAGCATAGACAACATCCA+TGG	0.629842	3.3:+50022247	None:intergenic
TGAGCACGCAAGGAGGAAGA+CGG	0.652638	3.3:-50022276	MS.gene08122:CDS
GTTCTTCGTGACAACATCCA+GGG	0.676380	3.3:-50022434	MS.gene08122:CDS
TTGATATATGAAGAAACCAG+AGG	0.683025	3.3:-50022350	MS.gene08122:CDS
CATACACTGAGCACGCAAGG+AGG	0.730442	3.3:-50022283	MS.gene08122:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGGTGTATTGAAGATATTCT+TGG	-	chr3.3:50022375-50022394	MS.gene08122:CDS	30.0%
	TTGATATATGAAGAAACCAG+AGG	-	chr3.3:50022355-50022374	MS.gene08122:CDS	30.0%
	GAATATCTTCAATACACCTC+TGG	+	chr3.3:50022374-50022393	None:intergenic	35.0%
	CAAAGCATAGACAACATCCA+TGG	+	chr3.3:50022461-50022480	None:intergenic	40.0%
!!	GTCTATGCTTTGAAGAGACA+GGG	-	chr3.3:50022469-50022488	MS.gene08122:CDS	40.0%
!!	TGTCTATGCTTTGAAGAGAC+AGG	-	chr3.3:50022468-50022487	MS.gene08122:CDS	40.0%
	AGGTGCAAAGAGACATAGGA+AGG	-	chr3.3:50022249-50022268	MS.gene08122:CDS	45.0%
	GGAAGAACGCTCTATGGTTT+CGG	-	chr3.3:50022490-50022509	MS.gene08122:CDS	45.0%
	GTTCTTCGTGACAACATCCA+GGG	-	chr3.3:50022271-50022290	MS.gene08122:CDS	45.0%
	AGAACGCTCTATGGTTTCGG+AGG	-	chr3.3:50022493-50022512	MS.gene08122:CDS	50.0%
	AGACAGGGAAGAACGCTCTA+TGG	-	chr3.3:50022484-50022503	MS.gene08122:CDS	50.0%
	GGTGGTGTGAAGAGAATCAG+TGG	-	chr3.3:50022331-50022350	MS.gene08122:CDS	50.0%
	GGTTCTTCGTGACAACATCC+AGG	-	chr3.3:50022270-50022289	MS.gene08122:CDS	50.0%
	TGACATACACTGAGCACGCA+AGG	-	chr3.3:50022419-50022438	MS.gene08122:CDS	50.0%
!	AGGGAGGTGCAAAGAGACAT+AGG	-	chr3.3:50022245-50022264	MS.gene08122:CDS	50.0%
	ATGTCAGGTCGTGGAAAGGG+GGG	-	chr3.3:50022208-50022227	MS.gene08122:CDS	55.0%
	CAAGCCTGCTATTCGCCGAT+TGG	-	chr3.3:50022300-50022319	MS.gene08122:CDS	55.0%
	CAATCGGCGAATAGCAGGCT+TGG	+	chr3.3:50022302-50022321	None:intergenic	55.0%
	CACCACCTCTTCTCGCCAAT+CGG	+	chr3.3:50022318-50022337	None:intergenic	55.0%
	CATACACTGAGCACGCAAGG+AGG	-	chr3.3:50022422-50022441	MS.gene08122:CDS	55.0%
	TGAGCACGCAAGGAGGAAGA+CGG	-	chr3.3:50022429-50022448	MS.gene08122:CDS	55.0%
!	ATTCGCCGATTGGCGAGAAG+AGG	-	chr3.3:50022310-50022329	MS.gene08122:CDS	55.0%
!!	TAGCAGGCTTGGTGATACCC+TGG	+	chr3.3:50022291-50022310	None:intergenic	55.0%
	AGGTCGTGGAAAGGGGGGTA+AGG	-	chr3.3:50022213-50022232	MS.gene08122:CDS	60.0%
	CTCGCCAATCGGCGAATAGC+AGG	+	chr3.3:50022307-50022326	None:intergenic	60.0%
	GAGGAAGACGGTGACAGCCA+TGG	-	chr3.3:50022441-50022460	MS.gene08122:CDS	60.0%
	GGAAAGGGGGGTAAGGGTCT+GGG	-	chr3.3:50022220-50022239	MS.gene08122:CDS	60.0%
	GGGGGTAAGGGTCTGGGAAA+GGG	-	chr3.3:50022226-50022245	MS.gene08122:CDS	60.0%
	GGTAAGGGTCTGGGAAAGGG+AGG	-	chr3.3:50022229-50022248	MS.gene08122:CDS	60.0%
	GGTCGTGGAAAGGGGGGTAA+GGG	-	chr3.3:50022214-50022233	MS.gene08122:CDS	60.0%
	TGGAAAGGGGGGTAAGGGTC+TGG	-	chr3.3:50022219-50022238	MS.gene08122:CDS	60.0%
	GGGGGGTAAGGGTCTGGGAA+AGG	-	chr3.3:50022225-50022244	MS.gene08122:CDS	65.0%
!	CGCCGATTGGCGAGAAGAGG+TGG	-	chr3.3:50022313-50022332	MS.gene08122:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	50022208	50022519	50022208	ID=MS.gene08122
chr3.3	mRNA	50022208	50022519	50022208	ID=MS.gene08122.t1;Parent=MS.gene08122
chr3.3	exon	50022208	50022519	50022208	ID=MS.gene08122.t1.exon1;Parent=MS.gene08122.t1
chr3.3	CDS	50022208	50022519	50022208	ID=cds.MS.gene08122.t1;Parent=MS.gene08122.t1

Gene Sequence	>MS.gene08122 ATGTCAGGTCGTGGAAAGGGGGGTAAGGGTCTGGGAAAGGGAGGTGCAAAGAGACATAGGAAGGTTCTTCGTGACAACATCCAGGGTATCACCAAGCCTGCTATTCGCCGATTGGCGAGAAGAGGTGGTGTGAAGAGAATCAGTGGATTGATATATGAAGAAACCAGAGGTGTATTGAAGATATTCTTGGAAAACGTCATTCGTGATGCAGTGACATACACTGAGCACGCAAGGAGGAAGACGGTGACAGCCATGGATGTTGTCTATGCTTTGAAGAGACAGGGAAGAACGCTCTATGGTTTCGGAGGTTGA
Protein sequence	>MS.gene08122.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG