AlfalfaGEDB | MS.gene010220

M. sativa cultivar XinJiangDaYe / MS.gene010220

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010220.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010220.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010220.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene010220	MS.gene008276	PPI
MS.gene08121	MS.gene010220	PPI
MS.gene010220	MS.gene73136	PPI
MS.gene022932	MS.gene010220	PPI
MS.gene010220	MS.gene049821	PPI
MS.gene010220	MS.gene04068	PPI
MS.gene88681	MS.gene010220	PPI
MS.gene010220	MS.gene022946	PPI
MS.gene010220	MS.gene05623	PPI
MS.gene035718	MS.gene010220	PPI
MS.gene035896	MS.gene010220	PPI
MS.gene010220	MS.gene66225	PPI
MS.gene010220	MS.gene035720	PPI
MS.gene010220	MS.gene61017	PPI
MS.gene009027	MS.gene010220	PPI
MS.gene010220	MS.gene09871	PPI
MS.gene78578	MS.gene010220	PPI
MS.gene060465	MS.gene010220	PPI
MS.gene010220	MS.gene049705	PPI
MS.gene043967	MS.gene010220	PPI
MS.gene010220	MS.gene04434	PPI
MS.gene010220	MS.gene070907	PPI
MS.gene010220	MS.gene04392	PPI
MS.gene028629	MS.gene010220	PPI
MS.gene010220	MS.gene010219	PPI
MS.gene010220	MS.gene05608	PPI
MS.gene010220	MS.gene68545	PPI
MS.gene010220	MS.gene070242	PPI
MS.gene010220	MS.gene016624	PPI
MS.gene04067	MS.gene010220	PPI
MS.gene71215	MS.gene010220	PPI
MS.gene010220	MS.gene82546	PPI
MS.gene061870	MS.gene010220	PPI
MS.gene010220	MS.gene048746	PPI
MS.gene010220	MS.gene09873	PPI
MS.gene66225	MS.gene010220	PPI
MS.gene071136	MS.gene010220	PPI
MS.gene070242	MS.gene010220	PPI
MS.gene010220	MS.gene043967	PPI
MS.gene035721	MS.gene010220	PPI
MS.gene010220	MS.gene62520	PPI
MS.gene010220	MS.gene035897	PPI
MS.gene010220	MS.gene035721	PPI
MS.gene056215	MS.gene010220	PPI
MS.gene064557	MS.gene010220	PPI
MS.gene038469	MS.gene010220	PPI
MS.gene04433	MS.gene010220	PPI
MS.gene04452	MS.gene010220	PPI
MS.gene010220	MS.gene072493	PPI
MS.gene035787	MS.gene010220	PPI
MS.gene010220	MS.gene84536	PPI
MS.gene010220	MS.gene035896	PPI
MS.gene89487	MS.gene010220	PPI
MS.gene010220	MS.gene64650	PPI
MS.gene010220	MS.gene60093	PPI
MS.gene035719	MS.gene010220	PPI
MS.gene010220	MS.gene08122	PPI
MS.gene58854	MS.gene010220	PPI
MS.gene010220	MS.gene061869	PPI
MS.gene010220	MS.gene022932	PPI
MS.gene70955	MS.gene010220	PPI
MS.gene040383	MS.gene010220	PPI
MS.gene30920	MS.gene010220	PPI
MS.gene08053	MS.gene010220	PPI
MS.gene061872	MS.gene010220	PPI
MS.gene010220	MS.gene04972	PPI
MS.gene010220	MS.gene004121	PPI
MS.gene88680	MS.gene010220	PPI
MS.gene010220	MS.gene018578	PPI
MS.gene070241	MS.gene010220	PPI
MS.gene010220	MS.gene061867	PPI
MS.gene010220	MS.gene04064	PPI
MS.gene010220	MS.gene018580	PPI
MS.gene010220	MS.gene004124	PPI
MS.gene010220	MS.gene05619	PPI
MS.gene061876	MS.gene010220	PPI
MS.gene012391	MS.gene010220	PPI
MS.gene010220	MS.gene04052	PPI
MS.gene070244	MS.gene010220	PPI
MS.gene010220	MS.gene89488	PPI
MS.gene010220	MS.gene061866	PPI
MS.gene64920	MS.gene010220	PPI
MS.gene060463	MS.gene010220	PPI
MS.gene010220	MS.gene79195	PPI
MS.gene010220	MS.gene048705	PPI
MS.gene010220	MS.gene061874	PPI
MS.gene010220	MS.gene04576	PPI
MS.gene31196	MS.gene010220	PPI
MS.gene010220	MS.gene012390	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010220.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010220.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010220.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010220.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010220.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010220.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010220.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010220.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010220.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010220.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010220.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010220.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010220.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010220.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010220.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene010220.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010220.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene010220.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene010220.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene010220.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene010220.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene010220.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene010220.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene010220.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene010220.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene010220.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene010220.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 29 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAAGGACCCTTTATGGTTT+TGG	0.248657	5.1:-48821231	MS.gene010220:CDS
AGGCAAGGAAGGACCCTTTA+TGG	0.289623	5.1:-48821237	MS.gene010220:CDS
AGGAAAAGGAGGAAAGGGTT+TGG	0.356920	5.1:-48821502	MS.gene010220:CDS
GGAAAAGGAGGAAAGGGTTT+GGG	0.372310	5.1:-48821501	MS.gene010220:CDS
AAGACGACGAATCGCGGGTT+TGG	0.418134	5.1:+48821422	None:intergenic
GGAAAGGGTTTGGGAAAGGG+TGG	0.425706	5.1:-48821492	MS.gene010220:CDS
GTTCTTAGAGATAACATTCA+AGG	0.432889	5.1:-48821450	MS.gene010220:CDS
GGAGGAAAGGGTTTGGGAAA+GGG	0.445686	5.1:-48821495	MS.gene010220:CDS
GGAAGAGGAAAAGGAGGAAA+GGG	0.454700	5.1:-48821507	MS.gene010220:CDS
TCGCAAAACGGTTACTGCTA+TGG	0.471375	5.1:-48821280	MS.gene010220:CDS
TGTTATCTCTAAGAACTTTG+CGG	0.472741	5.1:+48821457	None:intergenic
ATCTTGCAATCAACAATGTC+AGG	0.473641	5.1:-48821528	None:intergenic
AGGAGGAAAGGGTTTGGGAA+AGG	0.475381	5.1:-48821496	MS.gene010220:CDS
AGGAAGAGGAAAAGGAGGAA+AGG	0.488546	5.1:-48821508	MS.gene010220:CDS
ACAATGTCAGGAAGAGGAAA+AGG	0.493795	5.1:-48821516	MS.gene010220:CDS
GCGACGAGCGTGTTCAGTGT+AGG	0.513830	5.1:+48821299	None:intergenic
CCGCGATTCGTCGTCTTGCA+AGG	0.525617	5.1:-48821416	MS.gene010220:CDS
CAATCAACAATGTCAGGAAG+AGG	0.542220	5.1:-48821522	None:intergenic
CTTGCAAGACGACGAATCGC+GGG	0.554223	5.1:+48821417	None:intergenic
TGAACACGCTCGTCGCAAAA+CGG	0.560087	5.1:-48821292	MS.gene010220:CDS
CCTTGCAAGACGACGAATCG+CGG	0.571292	5.1:+48821416	None:intergenic
ATGCACTCAAGAGGCAAGGA+AGG	0.583106	5.1:-48821248	MS.gene010220:CDS
GTTTATGCACTCAAGAGGCA+AGG	0.602465	5.1:-48821252	MS.gene010220:CDS
CGTCGTCTTGCAAGGCGTGG+TGG	0.615268	5.1:-48821408	MS.gene010220:CDS
ATTCGTCGTCTTGCAAGGCG+TGG	0.619919	5.1:-48821411	MS.gene010220:CDS
ATGTTGTTTATGCACTCAAG+AGG	0.622074	5.1:-48821257	MS.gene010220:CDS
ATGTCAGGAAGAGGAAAAGG+AGG	0.630961	5.1:-48821513	MS.gene010220:CDS
GGTGGTGTGAAGCGTATCAG+TGG	0.661880	5.1:-48821390	MS.gene010220:CDS
CTCATCTATGAAGAAACTCG+TGG	0.682677	5.1:-48821366	MS.gene010220:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GTTCTTAGAGATAACATTCA+AGG	-	chr5.1:48821287-48821306	MS.gene010220:CDS	30.0%
!	TGTTATCTCTAAGAACTTTG+CGG	+	chr5.1:48821283-48821302	None:intergenic	30.0%
!	ATGTTGTTTATGCACTCAAG+AGG	-	chr5.1:48821480-48821499	MS.gene010220:CDS	35.0%
	CTAACCCCCAAAACCATAAA+GGG	+	chr5.1:48821516-48821535	None:intergenic	40.0%
	CTCATCTATGAAGAAACTCG+TGG	-	chr5.1:48821371-48821390	MS.gene010220:CDS	40.0%
!	GAAGGACCCTTTATGGTTTT+GGG	-	chr5.1:48821507-48821526	MS.gene010220:CDS	40.0%
!!	AAGGACCCTTTATGGTTTTG+GGG	-	chr5.1:48821508-48821527	MS.gene010220:CDS	40.0%
	AGGAAAAGGAGGAAAGGGTT+TGG	-	chr5.1:48821235-48821254	MS.gene010220:CDS	45.0%
	AGGAAGAGGAAAAGGAGGAA+AGG	-	chr5.1:48821229-48821248	MS.gene010220:CDS	45.0%
	ATGTCAGGAAGAGGAAAAGG+AGG	-	chr5.1:48821224-48821243	MS.gene010220:CDS	45.0%
	GGAAAAGGAGGAAAGGGTTT+GGG	-	chr5.1:48821236-48821255	MS.gene010220:CDS	45.0%
	GGAAGAGGAAAAGGAGGAAA+GGG	-	chr5.1:48821230-48821249	MS.gene010220:CDS	45.0%
	GTTTATGCACTCAAGAGGCA+AGG	-	chr5.1:48821485-48821504	MS.gene010220:CDS	45.0%
	TCGCAAAACGGTTACTGCTA+TGG	-	chr5.1:48821457-48821476	MS.gene010220:CDS	45.0%
!	GGAAGGACCCTTTATGGTTT+TGG	-	chr5.1:48821506-48821525	MS.gene010220:CDS	45.0%
!!!	AGGACCCTTTATGGTTTTGG+GGG	-	chr5.1:48821509-48821528	MS.gene010220:CDS	45.0%
	AGGAGGAAAGGGTTTGGGAA+AGG	-	chr5.1:48821241-48821260	MS.gene010220:CDS	50.0%
	AGGCAAGGAAGGACCCTTTA+TGG	-	chr5.1:48821500-48821519	MS.gene010220:CDS	50.0%
	ATGCACTCAAGAGGCAAGGA+AGG	-	chr5.1:48821489-48821508	MS.gene010220:CDS	50.0%
	GGAGGAAAGGGTTTGGGAAA+GGG	-	chr5.1:48821242-48821261	MS.gene010220:CDS	50.0%
	TGAACACGCTCGTCGCAAAA+CGG	-	chr5.1:48821445-48821464	MS.gene010220:CDS	50.0%
	AAGACGACGAATCGCGGGTT+TGG	+	chr5.1:48821318-48821337	None:intergenic	55.0%
	ATTCGTCGTCTTGCAAGGCG+TGG	-	chr5.1:48821326-48821345	MS.gene010220:CDS	55.0%
	CCTTGCAAGACGACGAATCG+CGG	+	chr5.1:48821324-48821343	None:intergenic	55.0%
	CTTGCAAGACGACGAATCGC+GGG	+	chr5.1:48821323-48821342	None:intergenic	55.0%
	GGAAAGGGTTTGGGAAAGGG+TGG	-	chr5.1:48821245-48821264	MS.gene010220:CDS	55.0%
	GGTGGTGTGAAGCGTATCAG+TGG	-	chr5.1:48821347-48821366	MS.gene010220:CDS	55.0%
	CCGCGATTCGTCGTCTTGCA+AGG	-	chr5.1:48821321-48821340	MS.gene010220:CDS	60.0%
	GCGACGAGCGTGTTCAGTGT+AGG	+	chr5.1:48821441-48821460	None:intergenic	60.0%
	CGTCGTCTTGCAAGGCGTGG+TGG	-	chr5.1:48821329-48821348	MS.gene010220:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	48821224	48821535	48821224	ID=MS.gene010220
chr5.1	mRNA	48821224	48821535	48821224	ID=MS.gene010220.t1;Parent=MS.gene010220
chr5.1	exon	48821224	48821535	48821224	ID=MS.gene010220.t1.exon1;Parent=MS.gene010220.t1
chr5.1	CDS	48821224	48821535	48821224	ID=cds.MS.gene010220.t1;Parent=MS.gene010220.t1

Gene Sequence	>MS.gene010220 ATGTCAGGAAGAGGAAAAGGAGGAAAGGGTTTGGGAAAGGGTGGTGCAAAGCGACACCGCAAAGTTCTTAGAGATAACATTCAAGGTATCACCAAACCCGCGATTCGTCGTCTTGCAAGGCGTGGTGGTGTGAAGCGTATCAGTGGTCTCATCTATGAAGAAACTCGTGGTGTTCTCAAGATATTTCTCGAGAATGTGATTCGTGATGCTGTAACCTACACTGAACACGCTCGTCGCAAAACGGTTACTGCTATGGATGTTGTTTATGCACTCAAGAGGCAAGGAAGGACCCTTTATGGTTTTGGGGGTTAG
Protein sequence	>MS.gene010220.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG