AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072493


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072493.t1 MTR_7g029510 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene072493.t1 MTR_7g099610 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene072493.t1 MTR_5g063620 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene072493.t1 MTR_4g061827 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene072493.t1 MTR_4g061920 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene072493.t1 MTR_3g462400 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene072493.t1 MTR_3g054380 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene072493.t1 MTR_3g462730 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene072493.t1 MTR_3g462510 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene072493.t1 MTR_3g070920 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene072493.t1 MTR_3g061440 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene072493.t1 MTR_3g462410 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene072493.t1 MTR_2g096100 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene072493.t1 MTR_8g038460 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene072493.t1 MTR_4g128150 100.000 102 0 0 1 102 1 102 1.67e-66 204
MS.gene072493.t1 MTR_7g029520 84.000 50 8 0 27 76 1 50 2.15e-21 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072493.t1 AT5G59970 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene072493.t1 AT5G59690 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene072493.t1 AT5G59970 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene072493.t1 AT3G46320 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene072493.t1 AT3G45930 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene072493.t1 AT3G53730 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene072493.t1 AT2G28740 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene072493.t1 AT1G07660 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene072493.t1 AT1G07820 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene072493.t1 AT1G07820 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene072493.t1 AT1G07660 83.495 103 0 1 1 103 1 86 4.34e-51 155

Find 33 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGCAGGGAAGGACTTTATA+TGG 0.314150 7.3:+3810762 MS.gene072493:CDS
GGAAGGACTTTATATGGTTT+CGG 0.362238 7.3:+3810768 MS.gene072493:CDS
GGAAAAGGAGGAAAGGGATT+AGG 0.403262 7.3:+3810498 MS.gene072493:CDS
AAAATGTCAGGCAGAGGAAA+AGG 0.476544 7.3:+3810483 None:intergenic
GTGCTTCGTGACAACATTCA+AGG 0.477240 7.3:+3810549 MS.gene072493:CDS
GGCAGAGGAAAAGGAGGAAA+GGG 0.498699 7.3:+3810492 MS.gene072493:CDS
TCACTGCAAGCGAAAATGTC+AGG 0.502586 7.3:+3810471 None:intergenic
CTGCGATTCGTCGTCTTGCA+AGG 0.502831 7.3:+3810583 MS.gene072493:CDS
TGTTTATGCTCTGAAGAGGC+AGG 0.507468 7.3:+3810746 MS.gene072493:CDS
AGGAGGAAAGGGATTAGGAA+AGG 0.515958 7.3:+3810503 MS.gene072493:CDS
GGAGGAAAGGGATTAGGAAA+GGG 0.527356 7.3:+3810504 MS.gene072493:CDS
GGAAAGGGATTAGGAAAGGG+AGG 0.538569 7.3:+3810507 MS.gene072493:CDS
AAGACGACGAATCGCAGGCT+TGG 0.548379 7.3:-3810577 None:intergenic
CTTGCAAGACGACGAATCGC+AGG 0.552192 7.3:-3810582 None:intergenic
AGGCAGAGGAAAAGGAGGAA+AGG 0.557567 7.3:+3810491 MS.gene072493:CDS
ATGCTCTGAAGAGGCAGGGA+AGG 0.564763 7.3:+3810751 MS.gene072493:CDS
GAGGAAGACTGTTACCGCCA+TGG 0.588179 7.3:+3810719 MS.gene072493:CDS
CAAGCGAAAATGTCAGGCAG+AGG 0.590069 7.3:+3810477 None:intergenic
CATTCTCGAGGAAGATCTTG+AGG 0.597682 7.3:-3810659 None:intergenic
AGGACTTTATATGGTTTCGG+TGG 0.600172 7.3:+3810771 MS.gene072493:CDS
TTACATACACTGAACACGCT+AGG 0.600176 7.3:+3810697 MS.gene072493:CDS
GTTTATGCTCTGAAGAGGCA+GGG 0.605263 7.3:+3810747 MS.gene072493:CDS
ATTCGTCGTCTTGCAAGGCG+TGG 0.611450 7.3:+3810588 MS.gene072493:CDS
GGCGGTGTTAAGAGAATCAG+TGG 0.619776 7.3:+3810609 MS.gene072493:CDS
CAGAGCATAAACAACATCCA+TGG 0.631933 7.3:-3810736 None:intergenic
CATACACTGAACACGCTAGG+AGG 0.635713 7.3:+3810700 MS.gene072493:CDS
CGTCGTCTTGCAAGGCGTGG+CGG 0.636706 7.3:+3810591 MS.gene072493:CDS
ATGTCAGGCAGAGGAAAAGG+AGG 0.661131 7.3:+3810486 MS.gene072493:CDS
ATGTTGTTTATGCTCTGAAG+AGG 0.672733 7.3:+3810742 MS.gene072493:CDS
TAGGAAAGGGAGGAGCAAAG+AGG 0.691969 7.3:+3810517 MS.gene072493:CDS
CTCATATATGAAGAAACTCG+CGG 0.708309 7.3:+3810633 MS.gene072493:CDS
CATCACGAATCACATTCTCG+AGG 0.731876 7.3:-3810671 None:intergenic
AGCATAAACAACATCCATGG+CGG 0.735882 7.3:-3810733 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
CTCATATATGAAGAAACTCG+CGG + chr7.3:3810633-3810652 MS.gene072493:CDS 35.0%
GGAAGGACTTTATATGGTTT+CGG + chr7.3:3810768-3810787 MS.gene072493:CDS 35.0%
!! ATGTTGTTTATGCTCTGAAG+AGG + chr7.3:3810742-3810761 MS.gene072493:CDS 35.0%
AGCATAAACAACATCCATGG+CGG - chr7.3:3810736-3810755 None:intergenic 40.0%
AGGACTTTATATGGTTTCGG+TGG + chr7.3:3810771-3810790 MS.gene072493:CDS 40.0%
CAGAGCATAAACAACATCCA+TGG - chr7.3:3810739-3810758 None:intergenic 40.0%
TTACATACACTGAACACGCT+AGG + chr7.3:3810697-3810716 MS.gene072493:CDS 40.0%
AGGAGGAAAGGGATTAGGAA+AGG + chr7.3:3810503-3810522 MS.gene072493:CDS 45.0%
AGGCAGGGAAGGACTTTATA+TGG + chr7.3:3810762-3810781 MS.gene072493:CDS 45.0%
CATCACGAATCACATTCTCG+AGG - chr7.3:3810674-3810693 None:intergenic 45.0%
CATTCTCGAGGAAGATCTTG+AGG - chr7.3:3810662-3810681 None:intergenic 45.0%
GGAAAAGGAGGAAAGGGATT+AGG + chr7.3:3810498-3810517 MS.gene072493:CDS 45.0%
GGAGGAAAGGGATTAGGAAA+GGG + chr7.3:3810504-3810523 MS.gene072493:CDS 45.0%
GTGCTTCGTGACAACATTCA+AGG + chr7.3:3810549-3810568 MS.gene072493:CDS 45.0%
!! GTTTATGCTCTGAAGAGGCA+GGG + chr7.3:3810747-3810766 MS.gene072493:CDS 45.0%
!! TGTTTATGCTCTGAAGAGGC+AGG + chr7.3:3810746-3810765 MS.gene072493:CDS 45.0%
AGGCAGAGGAAAAGGAGGAA+AGG + chr7.3:3810491-3810510 MS.gene072493:CDS 50.0%
ATGTCAGGCAGAGGAAAAGG+AGG + chr7.3:3810486-3810505 MS.gene072493:CDS 50.0%
CATACACTGAACACGCTAGG+AGG + chr7.3:3810700-3810719 MS.gene072493:CDS 50.0%
GGAAAGGGATTAGGAAAGGG+AGG + chr7.3:3810507-3810526 MS.gene072493:CDS 50.0%
GGCAGAGGAAAAGGAGGAAA+GGG + chr7.3:3810492-3810511 MS.gene072493:CDS 50.0%
TAGGAAAGGGAGGAGCAAAG+AGG + chr7.3:3810517-3810536 MS.gene072493:CDS 50.0%
! GGCGGTGTTAAGAGAATCAG+TGG + chr7.3:3810609-3810628 MS.gene072493:CDS 50.0%
AAGACGACGAATCGCAGGCT+TGG - chr7.3:3810580-3810599 None:intergenic 55.0%
ATTCGTCGTCTTGCAAGGCG+TGG + chr7.3:3810588-3810607 MS.gene072493:CDS 55.0%
CTGCGATTCGTCGTCTTGCA+AGG + chr7.3:3810583-3810602 MS.gene072493:CDS 55.0%
CTTGCAAGACGACGAATCGC+AGG - chr7.3:3810585-3810604 None:intergenic 55.0%
GAGGAAGACTGTTACCGCCA+TGG + chr7.3:3810719-3810738 MS.gene072493:CDS 55.0%
!! ATGCTCTGAAGAGGCAGGGA+AGG + chr7.3:3810751-3810770 MS.gene072493:CDS 55.0%
CGTCGTCTTGCAAGGCGTGG+CGG + chr7.3:3810591-3810610 MS.gene072493:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 3810486 3810797 3810486 ID=MS.gene072493
chr7.3 mRNA 3810486 3810797 3810486 ID=MS.gene072493.t1;Parent=MS.gene072493
chr7.3 exon 3810486 3810797 3810486 ID=MS.gene072493.t1.exon1;Parent=MS.gene072493.t1
chr7.3 CDS 3810486 3810797 3810486 ID=cds.MS.gene072493.t1;Parent=MS.gene072493.t1
Gene Sequence

>MS.gene072493

ATGTCAGGCAGAGGAAAAGGAGGAAAGGGATTAGGAAAGGGAGGAGCAAAGAGGCACAGAAAAGTGCTTCGTGACAACATTCAAGGAATAACCAAGCCTGCGATTCGTCGTCTTGCAAGGCGTGGCGGTGTTAAGAGAATCAGTGGTCTCATATATGAAGAAACTCGCGGTGTCCTCAAGATCTTCCTCGAGAATGTGATTCGTGATGCTGTTACATACACTGAACACGCTAGGAGGAAGACTGTTACCGCCATGGATGTTGTTTATGCTCTGAAGAGGCAGGGAAGGACTTTATATGGTTTCGGTGGTTGA

Protein sequence

>MS.gene072493.t1

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG