Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072493.t1 | KCW52694.1 | 100 | 103 | 0 | 0 | 1 | 103 | 4 | 106 | 7.50E-48 | 199.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072493.t1 | P62785 | 100.0 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 1.8e-50 | 199.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072493.t1 | M5VRN0 | 100.0 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 5.4e-48 | 199.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene072493 | 0.832062 | 1.17E-55 | -1.69E-46 |
| MS.gene049350 | MS.gene072493 | 0.826163 | 3.15E-54 | -1.69E-46 |
| MS.gene049807 | MS.gene072493 | 0.823208 | 1.57E-53 | -1.69E-46 |
| MS.gene049821 | MS.gene072493 | 0.890841 | 6.84E-74 | -1.69E-46 |
| MS.gene049850 | MS.gene072493 | 0.821632 | 3.64E-53 | -1.69E-46 |
| MS.gene050020 | MS.gene072493 | 0.818151 | 2.28E-52 | -1.69E-46 |
| MS.gene050689 | MS.gene072493 | 0.815134 | 1.08E-51 | -1.69E-46 |
| MS.gene050807 | MS.gene072493 | 0.823789 | 1.15E-53 | -1.69E-46 |
| MS.gene050808 | MS.gene072493 | 0.800751 | 1.24E-48 | -1.69E-46 |
| MS.gene050838 | MS.gene072493 | 0.81702 | 4.10E-52 | -1.69E-46 |
| MS.gene050901 | MS.gene072493 | 0.942116 | 1.27E-101 | -1.69E-46 |
| MS.gene051247 | MS.gene072493 | 0.850612 | 1.52E-60 | -1.69E-46 |
| MS.gene051328 | MS.gene072493 | 0.81673 | 4.76E-52 | -1.69E-46 |
| MS.gene05157 | MS.gene072493 | 0.824899 | 6.29E-54 | -1.69E-46 |
| MS.gene051664 | MS.gene072493 | 0.818546 | 1.85E-52 | -1.69E-46 |
| MS.gene051700 | MS.gene072493 | 0.817757 | 2.79E-52 | -1.69E-46 |
| MS.gene051719 | MS.gene072493 | 0.850081 | 2.14E-60 | -1.69E-46 |
| MS.gene051720 | MS.gene072493 | 0.854798 | 9.68E-62 | -1.69E-46 |
| MS.gene051744 | MS.gene072493 | 0.817606 | 3.02E-52 | -1.69E-46 |
| MS.gene051975 | MS.gene072493 | 0.801179 | 1.02E-48 | -1.69E-46 |
| MS.gene052133 | MS.gene072493 | 0.840058 | 1.09E-57 | -1.69E-46 |
| MS.gene052516 | MS.gene072493 | 0.835396 | 1.72E-56 | -1.69E-46 |
| MS.gene052519 | MS.gene072493 | 0.810472 | 1.13E-50 | -1.69E-46 |
| MS.gene052947 | MS.gene072493 | 0.824894 | 6.30E-54 | -1.69E-46 |
| MS.gene053122 | MS.gene072493 | 0.800949 | 1.13E-48 | -1.69E-46 |
| MS.gene053144 | MS.gene072493 | 0.81878 | 1.64E-52 | -1.69E-46 |
| MS.gene053226 | MS.gene072493 | 0.834153 | 3.53E-56 | -1.69E-46 |
| MS.gene053563 | MS.gene072493 | 0.825313 | 5.02E-54 | -1.69E-46 |
| MS.gene053581 | MS.gene072493 | 0.875362 | 3.27E-68 | -1.69E-46 |
| MS.gene05364 | MS.gene072493 | 0.813659 | 2.29E-51 | -1.69E-46 |
| MS.gene054142 | MS.gene072493 | 0.80403 | 2.63E-49 | -1.69E-46 |
| MS.gene05423 | MS.gene072493 | 0.812131 | 4.95E-51 | -1.69E-46 |
| MS.gene054584 | MS.gene072493 | 0.801071 | 1.07E-48 | -1.69E-46 |
| MS.gene054651 | MS.gene072493 | 0.807527 | 4.84E-50 | -1.69E-46 |
| MS.gene055465 | MS.gene072493 | 0.842694 | 2.21E-58 | -1.69E-46 |
| MS.gene055466 | MS.gene072493 | 0.857905 | 1.19E-62 | -1.69E-46 |
| MS.gene055468 | MS.gene072493 | 0.840255 | 9.71E-58 | -1.69E-46 |
| MS.gene055469 | MS.gene072493 | 0.811004 | 8.70E-51 | -1.69E-46 |
| MS.gene055513 | MS.gene072493 | 0.827897 | 1.21E-54 | -1.69E-46 |
| MS.gene055537 | MS.gene072493 | 0.824538 | 7.65E-54 | -1.69E-46 |
| MS.gene055580 | MS.gene072493 | -0.831229 | 1.88E-55 | -1.69E-46 |
| MS.gene055818 | MS.gene072493 | 0.826946 | 2.05E-54 | -1.69E-46 |
| MS.gene056005 | MS.gene072493 | 0.829503 | 4.96E-55 | -1.69E-46 |
| MS.gene056043 | MS.gene072493 | -0.809642 | 1.71E-50 | -1.69E-46 |
| MS.gene05608 | MS.gene072493 | 0.835493 | 1.63E-56 | -1.69E-46 |
| MS.gene056174 | MS.gene072493 | 0.806071 | 9.83E-50 | -1.69E-46 |
| MS.gene056215 | MS.gene072493 | 0.856374 | 3.36E-62 | -1.69E-46 |
| MS.gene056308 | MS.gene072493 | 0.850197 | 1.98E-60 | -1.69E-46 |
| MS.gene056366 | MS.gene072493 | 0.803438 | 3.49E-49 | -1.69E-46 |
| MS.gene056595 | MS.gene072493 | 0.820363 | 7.14E-53 | -1.69E-46 |
| MS.gene056699 | MS.gene072493 | 0.802081 | 6.64E-49 | -1.69E-46 |
| MS.gene057034 | MS.gene072493 | 0.804953 | 1.69E-49 | -1.69E-46 |
| MS.gene057102 | MS.gene072493 | 0.807805 | 4.22E-50 | -1.69E-46 |
| MS.gene057294 | MS.gene072493 | 0.820711 | 5.94E-53 | -1.69E-46 |
| MS.gene057444 | MS.gene072493 | 0.818516 | 1.88E-52 | -1.69E-46 |
| MS.gene057693 | MS.gene072493 | 0.800764 | 1.24E-48 | -1.69E-46 |
| MS.gene057694 | MS.gene072493 | 0.834088 | 3.67E-56 | -1.69E-46 |
| MS.gene057705 | MS.gene072493 | 0.8354 | 1.72E-56 | -1.69E-46 |
| MS.gene058339 | MS.gene072493 | 0.802985 | 4.33E-49 | -1.69E-46 |
| MS.gene05847 | MS.gene072493 | 0.81523 | 1.03E-51 | -1.69E-46 |
| MS.gene058625 | MS.gene072493 | 0.811637 | 6.34E-51 | -1.69E-46 |
| MS.gene058852 | MS.gene072493 | 0.807349 | 5.28E-50 | -1.69E-46 |
| MS.gene05894 | MS.gene072493 | 0.824226 | 9.05E-54 | -1.69E-46 |
| MS.gene059227 | MS.gene072493 | 0.81172 | 6.08E-51 | -1.69E-46 |
| MS.gene059625 | MS.gene072493 | 0.805469 | 1.31E-49 | -1.69E-46 |
| MS.gene060365 | MS.gene072493 | 0.83174 | 1.41E-55 | -1.69E-46 |
| MS.gene060429 | MS.gene072493 | 0.84635 | 2.30E-59 | -1.69E-46 |
| MS.gene060519 | MS.gene072493 | 0.82864 | 8.04E-55 | -1.69E-46 |
| MS.gene060587 | MS.gene072493 | 0.832007 | 1.21E-55 | -1.69E-46 |
| MS.gene060917 | MS.gene072493 | 0.821375 | 4.18E-53 | -1.69E-46 |
| MS.gene061100 | MS.gene072493 | 0.828621 | 8.12E-55 | -1.69E-46 |
| MS.gene061176 | MS.gene072493 | 0.815072 | 1.11E-51 | -1.69E-46 |
| MS.gene061177 | MS.gene072493 | 0.814295 | 1.66E-51 | -1.69E-46 |
| MS.gene061178 | MS.gene072493 | 0.814843 | 1.25E-51 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072493.t1 | MTR_7g029510 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.85e-68 | 199 |
| MS.gene072493.t1 | MTR_7g099610 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.85e-68 | 199 |
| MS.gene072493.t1 | MTR_5g063620 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.85e-68 | 199 |
| MS.gene072493.t1 | MTR_4g061827 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.85e-68 | 199 |
| MS.gene072493.t1 | MTR_4g061920 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.85e-68 | 199 |
| MS.gene072493.t1 | MTR_3g462400 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.85e-68 | 199 |
| MS.gene072493.t1 | MTR_3g054380 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.85e-68 | 199 |
| MS.gene072493.t1 | MTR_3g462730 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.85e-68 | 199 |
| MS.gene072493.t1 | MTR_3g462510 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.85e-68 | 199 |
| MS.gene072493.t1 | MTR_3g070920 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.85e-68 | 199 |
| MS.gene072493.t1 | MTR_3g061440 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.85e-68 | 199 |
| MS.gene072493.t1 | MTR_3g462410 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.85e-68 | 199 |
| MS.gene072493.t1 | MTR_2g096100 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.85e-68 | 199 |
| MS.gene072493.t1 | MTR_8g038460 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.85e-68 | 199 |
| MS.gene072493.t1 | MTR_4g128150 | 100.000 | 102 | 0 | 0 | 1 | 102 | 1 | 102 | 1.67e-66 | 204 |
| MS.gene072493.t1 | MTR_7g029520 | 84.000 | 50 | 8 | 0 | 27 | 76 | 1 | 50 | 2.15e-21 | 87.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072493.t1 | AT5G59970 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.99e-68 | 199 |
| MS.gene072493.t1 | AT5G59690 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.99e-68 | 199 |
| MS.gene072493.t1 | AT5G59970 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.99e-68 | 199 |
| MS.gene072493.t1 | AT3G46320 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.99e-68 | 199 |
| MS.gene072493.t1 | AT3G45930 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.99e-68 | 199 |
| MS.gene072493.t1 | AT3G53730 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.99e-68 | 199 |
| MS.gene072493.t1 | AT2G28740 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.99e-68 | 199 |
| MS.gene072493.t1 | AT1G07660 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.99e-68 | 199 |
| MS.gene072493.t1 | AT1G07820 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.99e-68 | 199 |
| MS.gene072493.t1 | AT1G07820 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.99e-68 | 199 |
| MS.gene072493.t1 | AT1G07660 | 83.495 | 103 | 0 | 1 | 1 | 103 | 1 | 86 | 4.34e-51 | 155 |
Find 33 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGCAGGGAAGGACTTTATA+TGG | 0.314150 | 7.3:+3810762 | MS.gene072493:CDS |
| GGAAGGACTTTATATGGTTT+CGG | 0.362238 | 7.3:+3810768 | MS.gene072493:CDS |
| GGAAAAGGAGGAAAGGGATT+AGG | 0.403262 | 7.3:+3810498 | MS.gene072493:CDS |
| AAAATGTCAGGCAGAGGAAA+AGG | 0.476544 | 7.3:+3810483 | None:intergenic |
| GTGCTTCGTGACAACATTCA+AGG | 0.477240 | 7.3:+3810549 | MS.gene072493:CDS |
| GGCAGAGGAAAAGGAGGAAA+GGG | 0.498699 | 7.3:+3810492 | MS.gene072493:CDS |
| TCACTGCAAGCGAAAATGTC+AGG | 0.502586 | 7.3:+3810471 | None:intergenic |
| CTGCGATTCGTCGTCTTGCA+AGG | 0.502831 | 7.3:+3810583 | MS.gene072493:CDS |
| TGTTTATGCTCTGAAGAGGC+AGG | 0.507468 | 7.3:+3810746 | MS.gene072493:CDS |
| AGGAGGAAAGGGATTAGGAA+AGG | 0.515958 | 7.3:+3810503 | MS.gene072493:CDS |
| GGAGGAAAGGGATTAGGAAA+GGG | 0.527356 | 7.3:+3810504 | MS.gene072493:CDS |
| GGAAAGGGATTAGGAAAGGG+AGG | 0.538569 | 7.3:+3810507 | MS.gene072493:CDS |
| AAGACGACGAATCGCAGGCT+TGG | 0.548379 | 7.3:-3810577 | None:intergenic |
| CTTGCAAGACGACGAATCGC+AGG | 0.552192 | 7.3:-3810582 | None:intergenic |
| AGGCAGAGGAAAAGGAGGAA+AGG | 0.557567 | 7.3:+3810491 | MS.gene072493:CDS |
| ATGCTCTGAAGAGGCAGGGA+AGG | 0.564763 | 7.3:+3810751 | MS.gene072493:CDS |
| GAGGAAGACTGTTACCGCCA+TGG | 0.588179 | 7.3:+3810719 | MS.gene072493:CDS |
| CAAGCGAAAATGTCAGGCAG+AGG | 0.590069 | 7.3:+3810477 | None:intergenic |
| CATTCTCGAGGAAGATCTTG+AGG | 0.597682 | 7.3:-3810659 | None:intergenic |
| AGGACTTTATATGGTTTCGG+TGG | 0.600172 | 7.3:+3810771 | MS.gene072493:CDS |
| TTACATACACTGAACACGCT+AGG | 0.600176 | 7.3:+3810697 | MS.gene072493:CDS |
| GTTTATGCTCTGAAGAGGCA+GGG | 0.605263 | 7.3:+3810747 | MS.gene072493:CDS |
| ATTCGTCGTCTTGCAAGGCG+TGG | 0.611450 | 7.3:+3810588 | MS.gene072493:CDS |
| GGCGGTGTTAAGAGAATCAG+TGG | 0.619776 | 7.3:+3810609 | MS.gene072493:CDS |
| CAGAGCATAAACAACATCCA+TGG | 0.631933 | 7.3:-3810736 | None:intergenic |
| CATACACTGAACACGCTAGG+AGG | 0.635713 | 7.3:+3810700 | MS.gene072493:CDS |
| CGTCGTCTTGCAAGGCGTGG+CGG | 0.636706 | 7.3:+3810591 | MS.gene072493:CDS |
| ATGTCAGGCAGAGGAAAAGG+AGG | 0.661131 | 7.3:+3810486 | MS.gene072493:CDS |
| ATGTTGTTTATGCTCTGAAG+AGG | 0.672733 | 7.3:+3810742 | MS.gene072493:CDS |
| TAGGAAAGGGAGGAGCAAAG+AGG | 0.691969 | 7.3:+3810517 | MS.gene072493:CDS |
| CTCATATATGAAGAAACTCG+CGG | 0.708309 | 7.3:+3810633 | MS.gene072493:CDS |
| CATCACGAATCACATTCTCG+AGG | 0.731876 | 7.3:-3810671 | None:intergenic |
| AGCATAAACAACATCCATGG+CGG | 0.735882 | 7.3:-3810733 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTCATATATGAAGAAACTCG+CGG | + | chr7.3:3810633-3810652 | MS.gene072493:CDS | 35.0% | |
| GGAAGGACTTTATATGGTTT+CGG | + | chr7.3:3810768-3810787 | MS.gene072493:CDS | 35.0% | |
| !! | ATGTTGTTTATGCTCTGAAG+AGG | + | chr7.3:3810742-3810761 | MS.gene072493:CDS | 35.0% |
| AGCATAAACAACATCCATGG+CGG | - | chr7.3:3810736-3810755 | None:intergenic | 40.0% | |
| AGGACTTTATATGGTTTCGG+TGG | + | chr7.3:3810771-3810790 | MS.gene072493:CDS | 40.0% | |
| CAGAGCATAAACAACATCCA+TGG | - | chr7.3:3810739-3810758 | None:intergenic | 40.0% | |
| TTACATACACTGAACACGCT+AGG | + | chr7.3:3810697-3810716 | MS.gene072493:CDS | 40.0% | |
| AGGAGGAAAGGGATTAGGAA+AGG | + | chr7.3:3810503-3810522 | MS.gene072493:CDS | 45.0% | |
| AGGCAGGGAAGGACTTTATA+TGG | + | chr7.3:3810762-3810781 | MS.gene072493:CDS | 45.0% | |
| CATCACGAATCACATTCTCG+AGG | - | chr7.3:3810674-3810693 | None:intergenic | 45.0% | |
| CATTCTCGAGGAAGATCTTG+AGG | - | chr7.3:3810662-3810681 | None:intergenic | 45.0% | |
| GGAAAAGGAGGAAAGGGATT+AGG | + | chr7.3:3810498-3810517 | MS.gene072493:CDS | 45.0% | |
| GGAGGAAAGGGATTAGGAAA+GGG | + | chr7.3:3810504-3810523 | MS.gene072493:CDS | 45.0% | |
| GTGCTTCGTGACAACATTCA+AGG | + | chr7.3:3810549-3810568 | MS.gene072493:CDS | 45.0% | |
| !! | GTTTATGCTCTGAAGAGGCA+GGG | + | chr7.3:3810747-3810766 | MS.gene072493:CDS | 45.0% |
| !! | TGTTTATGCTCTGAAGAGGC+AGG | + | chr7.3:3810746-3810765 | MS.gene072493:CDS | 45.0% |
| AGGCAGAGGAAAAGGAGGAA+AGG | + | chr7.3:3810491-3810510 | MS.gene072493:CDS | 50.0% | |
| ATGTCAGGCAGAGGAAAAGG+AGG | + | chr7.3:3810486-3810505 | MS.gene072493:CDS | 50.0% | |
| CATACACTGAACACGCTAGG+AGG | + | chr7.3:3810700-3810719 | MS.gene072493:CDS | 50.0% | |
| GGAAAGGGATTAGGAAAGGG+AGG | + | chr7.3:3810507-3810526 | MS.gene072493:CDS | 50.0% | |
| GGCAGAGGAAAAGGAGGAAA+GGG | + | chr7.3:3810492-3810511 | MS.gene072493:CDS | 50.0% | |
| TAGGAAAGGGAGGAGCAAAG+AGG | + | chr7.3:3810517-3810536 | MS.gene072493:CDS | 50.0% | |
| ! | GGCGGTGTTAAGAGAATCAG+TGG | + | chr7.3:3810609-3810628 | MS.gene072493:CDS | 50.0% |
| AAGACGACGAATCGCAGGCT+TGG | - | chr7.3:3810580-3810599 | None:intergenic | 55.0% | |
| ATTCGTCGTCTTGCAAGGCG+TGG | + | chr7.3:3810588-3810607 | MS.gene072493:CDS | 55.0% | |
| CTGCGATTCGTCGTCTTGCA+AGG | + | chr7.3:3810583-3810602 | MS.gene072493:CDS | 55.0% | |
| CTTGCAAGACGACGAATCGC+AGG | - | chr7.3:3810585-3810604 | None:intergenic | 55.0% | |
| GAGGAAGACTGTTACCGCCA+TGG | + | chr7.3:3810719-3810738 | MS.gene072493:CDS | 55.0% | |
| !! | ATGCTCTGAAGAGGCAGGGA+AGG | + | chr7.3:3810751-3810770 | MS.gene072493:CDS | 55.0% |
| CGTCGTCTTGCAAGGCGTGG+CGG | + | chr7.3:3810591-3810610 | MS.gene072493:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 3810486 | 3810797 | 3810486 | ID=MS.gene072493 |
| chr7.3 | mRNA | 3810486 | 3810797 | 3810486 | ID=MS.gene072493.t1;Parent=MS.gene072493 |
| chr7.3 | exon | 3810486 | 3810797 | 3810486 | ID=MS.gene072493.t1.exon1;Parent=MS.gene072493.t1 |
| chr7.3 | CDS | 3810486 | 3810797 | 3810486 | ID=cds.MS.gene072493.t1;Parent=MS.gene072493.t1 |
| Gene Sequence |
| Protein sequence |