AlfalfaGEDB | MS.gene04068

M. sativa cultivar XinJiangDaYe / MS.gene04068

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04068.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04068.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04068.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene04452	MS.gene04068	PPI
MS.gene035787	MS.gene04068	PPI
MS.gene04068	MS.gene04972	PPI
MS.gene04068	MS.gene004121	PPI
MS.gene89487	MS.gene04068	PPI
MS.gene04068	MS.gene018578	PPI
MS.gene035719	MS.gene04068	PPI
MS.gene04068	MS.gene061867	PPI
MS.gene58854	MS.gene04068	PPI
MS.gene04068	MS.gene04064	PPI
MS.gene04068	MS.gene018580	PPI
MS.gene04068	MS.gene004124	PPI
MS.gene04392	MS.gene04068	PPI
MS.gene072493	MS.gene04068	PPI
MS.gene30920	MS.gene04068	PPI
MS.gene04068	MS.gene05619	PPI
MS.gene048746	MS.gene04068	PPI
MS.gene010220	MS.gene04068	PPI
MS.gene08053	MS.gene04068	PPI
MS.gene061872	MS.gene04068	PPI
MS.gene04068	MS.gene04052	PPI
MS.gene04068	MS.gene89488	PPI
MS.gene04068	MS.gene061866	PPI
MS.gene88680	MS.gene04068	PPI
MS.gene070241	MS.gene04068	PPI
MS.gene04434	MS.gene04068	PPI
MS.gene022946	MS.gene04068	PPI
MS.gene04068	MS.gene79195	PPI
MS.gene04068	MS.gene048705	PPI
MS.gene04068	MS.gene061874	PPI
MS.gene04068	MS.gene04576	PPI
MS.gene04068	MS.gene012390	PPI
MS.gene061876	MS.gene04068	PPI
MS.gene012391	MS.gene04068	PPI
MS.gene88681	MS.gene04068	PPI
MS.gene070244	MS.gene04068	PPI
MS.gene09871	MS.gene04068	PPI
MS.gene035897	MS.gene04068	PPI
MS.gene64920	MS.gene04068	PPI
MS.gene060463	MS.gene04068	PPI
MS.gene31196	MS.gene04068	PPI
MS.gene04068	MS.gene016624	PPI
MS.gene04068	MS.gene008276	PPI
MS.gene060465	MS.gene04068	PPI
MS.gene04068	MS.gene73136	PPI
MS.gene04068	MS.gene049821	PPI
MS.gene04068	MS.gene05623	PPI
MS.gene04068	MS.gene035720	PPI
MS.gene04068	MS.gene61017	PPI
MS.gene04068	MS.gene09871	PPI
MS.gene08121	MS.gene04068	PPI
MS.gene022932	MS.gene04068	PPI
MS.gene04068	MS.gene049705	PPI
MS.gene04068	MS.gene04434	PPI
MS.gene035718	MS.gene04068	PPI
MS.gene04068	MS.gene070907	PPI
MS.gene035896	MS.gene04068	PPI
MS.gene04068	MS.gene04392	PPI
MS.gene04068	MS.gene010219	PPI
MS.gene04068	MS.gene05608	PPI
MS.gene009027	MS.gene04068	PPI
MS.gene04068	MS.gene68545	PPI
MS.gene78578	MS.gene04068	PPI
MS.gene04068	MS.gene82546	PPI
MS.gene05623	MS.gene04068	PPI
MS.gene043967	MS.gene04068	PPI
MS.gene04068	MS.gene048746	PPI
MS.gene028629	MS.gene04068	PPI
MS.gene04068	MS.gene09873	PPI
MS.gene04433	MS.gene04068	PPI
MS.gene04067	MS.gene04068	PPI
MS.gene71215	MS.gene04068	PPI
MS.gene061870	MS.gene04068	PPI
MS.gene04068	MS.gene62520	PPI
MS.gene04068	MS.gene035897	PPI
MS.gene66225	MS.gene04068	PPI
MS.gene70955	MS.gene04068	PPI
MS.gene071136	MS.gene04068	PPI
MS.gene040383	MS.gene04068	PPI
MS.gene070242	MS.gene04068	PPI
MS.gene79195	MS.gene04068	PPI
MS.gene035721	MS.gene04068	PPI
MS.gene04068	MS.gene84536	PPI
MS.gene056215	MS.gene04068	PPI
MS.gene064557	MS.gene04068	PPI
MS.gene04068	MS.gene64650	PPI
MS.gene038469	MS.gene04068	PPI
MS.gene04068	MS.gene60093	PPI
MS.gene04068	MS.gene08122	PPI
MS.gene04068	MS.gene061869	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04068.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04068.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04068.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04068.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04068.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04068.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04068.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04068.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04068.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04068.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04068.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04068.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04068.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04068.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04068.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene04068.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04068.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04068.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04068.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04068.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04068.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04068.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04068.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04068.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04068.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04068.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04068.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 38 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAAGAACCCTCTATGGATT+TGG	0.370047	3.1:-46701158	MS.gene04068:CDS
AGACAAGGAAGAACCCTCTA+TGG	0.396310	3.1:-46701164	MS.gene04068:CDS
AGGTGTCTTGAAGATCTTCT+TGG	0.405705	3.1:-46701273	MS.gene04068:CDS
TTCAACCTCCAAATCCATAG+AGG	0.435462	3.1:+46701150	None:intergenic
CAATCGACGAATAGCAGGTT+TGG	0.439411	3.1:+46701349	None:intergenic
CTTGCCAATCGACGAATAGC+AGG	0.487494	3.1:+46701344	None:intergenic
GGTAAGGGACTCGGAAAGGG+AGG	0.488695	3.1:-46701419	MS.gene04068:CDS
AGGCCGTGGAAAGGGAGGTA+AGG	0.492819	3.1:-46701435	MS.gene04068:CDS
AGGGAGGTGCAAAGAGGCAT+AGG	0.498985	3.1:-46701403	MS.gene04068:CDS
GGAGGTAAGGGACTCGGAAA+GGG	0.499726	3.1:-46701422	MS.gene04068:CDS
AGAACCCTCTATGGATTTGG+AGG	0.508817	3.1:-46701155	MS.gene04068:CDS
CTCGCGAAAATGTCAGGCCG+TGG	0.510433	3.1:-46701449	None:intergenic
GAAGATCTTCAAGACACCTC+TGG	0.510902	3.1:+46701277	None:intergenic
GTCCCTTACCTCCCTTTCCA+CGG	0.513750	3.1:+46701432	None:intergenic
AAAATGTCAGGCCGTGGAAA+GGG	0.516096	3.1:-46701443	MS.gene04068:CDS
GGGAGGTAAGGGACTCGGAA+AGG	0.518226	3.1:-46701423	MS.gene04068:CDS
TCACATACACAGAGCATGCT+AGG	0.524957	3.1:-46701229	MS.gene04068:CDS
GGTTCTCCGTGATAACATCC+AGG	0.528902	3.1:-46701378	MS.gene04068:CDS
GGAAAGGGAGGTAAGGGACT+CGG	0.535187	3.1:-46701428	MS.gene04068:CDS
GAGGAAGACGGTGACAGCCA+TGG	0.546349	3.1:-46701207	MS.gene04068:CDS
GGCCGTGGAAAGGGAGGTAA+GGG	0.552535	3.1:-46701434	MS.gene04068:CDS
CAAACCTGCTATTCGTCGAT+TGG	0.553041	3.1:-46701348	MS.gene04068:CDS
GAAAATGTCAGGCCGTGGAA+AGG	0.558967	3.1:-46701444	None:intergenic
GTCTATGCTTTGAAGAGACA+AGG	0.570048	3.1:-46701179	MS.gene04068:CDS
AGGTGCAAAGAGGCATAGGA+AGG	0.586086	3.1:-46701399	MS.gene04068:CDS
CGTCGATTGGCAAGAAGAGG+TGG	0.593946	3.1:-46701335	MS.gene04068:CDS
TAGCAGGTTTGGTGATACCC+TGG	0.596796	3.1:+46701360	None:intergenic
TCAACCTCCAAATCCATAGA+GGG	0.597347	3.1:+46701151	None:intergenic
GGTGGTGTCAAGAGAATCAG+TGG	0.607763	3.1:-46701317	MS.gene04068:CDS
TGATACCCTGGATGTTATCA+CGG	0.616496	3.1:+46701372	None:intergenic
ATTCGTCGATTGGCAAGAAG+AGG	0.629429	3.1:-46701338	MS.gene04068:CDS
CAAAGCATAGACAACATCCA+TGG	0.629842	3.1:+46701190	None:intergenic
ATGTCAGGCCGTGGAAAGGG+AGG	0.634999	3.1:-46701440	MS.gene04068:CDS
GTTCTCCGTGATAACATCCA+GGG	0.667356	3.1:-46701377	MS.gene04068:CDS
AGAGCATGCTAGGAGGAAGA+CGG	0.676878	3.1:-46701219	MS.gene04068:CDS
TCGGAAAGGGAGGTGCAAAG+AGG	0.683014	3.1:-46701409	MS.gene04068:CDS
TTGATCTATGAAGAAACCAG+AGG	0.704889	3.1:-46701293	MS.gene04068:CDS
CATACACAGAGCATGCTAGG+AGG	0.705154	3.1:-46701226	MS.gene04068:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGATCTATGAAGAAACCAG+AGG	-	chr3.1:46701298-46701317	MS.gene04068:CDS	35.0%
	AGGTGTCTTGAAGATCTTCT+TGG	-	chr3.1:46701318-46701337	MS.gene04068:CDS	40.0%
	CAAAGCATAGACAACATCCA+TGG	+	chr3.1:46701404-46701423	None:intergenic	40.0%
	TCAACCTCCAAATCCATAGA+GGG	+	chr3.1:46701443-46701462	None:intergenic	40.0%
	TGATACCCTGGATGTTATCA+CGG	+	chr3.1:46701222-46701241	None:intergenic	40.0%
!!	GTCTATGCTTTGAAGAGACA+AGG	-	chr3.1:46701412-46701431	MS.gene04068:CDS	40.0%
	AGAACCCTCTATGGATTTGG+AGG	-	chr3.1:46701436-46701455	MS.gene04068:CDS	45.0%
	AGACAAGGAAGAACCCTCTA+TGG	-	chr3.1:46701427-46701446	MS.gene04068:CDS	45.0%
	CAAACCTGCTATTCGTCGAT+TGG	-	chr3.1:46701243-46701262	MS.gene04068:CDS	45.0%
	CAATCGACGAATAGCAGGTT+TGG	+	chr3.1:46701245-46701264	None:intergenic	45.0%
	GAAGATCTTCAAGACACCTC+TGG	+	chr3.1:46701317-46701336	None:intergenic	45.0%
	GGAAGAACCCTCTATGGATT+TGG	-	chr3.1:46701433-46701452	MS.gene04068:CDS	45.0%
	GTTCTCCGTGATAACATCCA+GGG	-	chr3.1:46701214-46701233	MS.gene04068:CDS	45.0%
	TCACATACACAGAGCATGCT+AGG	-	chr3.1:46701362-46701381	MS.gene04068:CDS	45.0%
!	ATTCGTCGATTGGCAAGAAG+AGG	-	chr3.1:46701253-46701272	MS.gene04068:CDS	45.0%
	AGAGCATGCTAGGAGGAAGA+CGG	-	chr3.1:46701372-46701391	MS.gene04068:CDS	50.0%
	CATACACAGAGCATGCTAGG+AGG	-	chr3.1:46701365-46701384	MS.gene04068:CDS	50.0%
	CTTGCCAATCGACGAATAGC+AGG	+	chr3.1:46701250-46701269	None:intergenic	50.0%
	GGTTCTCCGTGATAACATCC+AGG	-	chr3.1:46701213-46701232	MS.gene04068:CDS	50.0%
!	AGGTGCAAAGAGGCATAGGA+AGG	-	chr3.1:46701192-46701211	MS.gene04068:CDS	50.0%
!	GGTGGTGTCAAGAGAATCAG+TGG	-	chr3.1:46701274-46701293	MS.gene04068:CDS	50.0%
!!	TAGCAGGTTTGGTGATACCC+TGG	+	chr3.1:46701234-46701253	None:intergenic	50.0%
	GGAAAGGGAGGTAAGGGACT+CGG	-	chr3.1:46701163-46701182	MS.gene04068:CDS	55.0%
	GGAGGTAAGGGACTCGGAAA+GGG	-	chr3.1:46701169-46701188	MS.gene04068:CDS	55.0%
	GTCCCTTACCTCCCTTTCCA+CGG	+	chr3.1:46701162-46701181	None:intergenic	55.0%
!	AGGGAGGTGCAAAGAGGCAT+AGG	-	chr3.1:46701188-46701207	MS.gene04068:CDS	55.0%
!	CGTCGATTGGCAAGAAGAGG+TGG	-	chr3.1:46701256-46701275	MS.gene04068:CDS	55.0%
!	TCGGAAAGGGAGGTGCAAAG+AGG	-	chr3.1:46701182-46701201	MS.gene04068:CDS	55.0%
	AGGCCGTGGAAAGGGAGGTA+AGG	-	chr3.1:46701156-46701175	MS.gene04068:CDS	60.0%
	ATGTCAGGCCGTGGAAAGGG+AGG	-	chr3.1:46701151-46701170	MS.gene04068:CDS	60.0%
	GAGGAAGACGGTGACAGCCA+TGG	-	chr3.1:46701384-46701403	MS.gene04068:CDS	60.0%
	GGCCGTGGAAAGGGAGGTAA+GGG	-	chr3.1:46701157-46701176	MS.gene04068:CDS	60.0%
	GGGAGGTAAGGGACTCGGAA+AGG	-	chr3.1:46701168-46701187	MS.gene04068:CDS	60.0%
	GGTAAGGGACTCGGAAAGGG+AGG	-	chr3.1:46701172-46701191	MS.gene04068:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	46701151	46701462	46701151	ID=MS.gene04068
chr3.1	mRNA	46701151	46701462	46701151	ID=MS.gene04068.t1;Parent=MS.gene04068
chr3.1	exon	46701151	46701462	46701151	ID=MS.gene04068.t1.exon1;Parent=MS.gene04068.t1
chr3.1	CDS	46701151	46701462	46701151	ID=cds.MS.gene04068.t1;Parent=MS.gene04068.t1

Gene Sequence	>MS.gene04068 ATGTCAGGCCGTGGAAAGGGAGGTAAGGGACTCGGAAAGGGAGGTGCAAAGAGGCATAGGAAGGTTCTCCGTGATAACATCCAGGGTATCACCAAACCTGCTATTCGTCGATTGGCAAGAAGAGGTGGTGTCAAGAGAATCAGTGGATTGATCTATGAAGAAACCAGAGGTGTCTTGAAGATCTTCTTGGAAAACGTCATTCGTGATGCTGTCACATACACAGAGCATGCTAGGAGGAAGACGGTGACAGCCATGGATGTTGTCTATGCTTTGAAGAGACAAGGAAGAACCCTCTATGGATTTGGAGGTTGA
Protein sequence	>MS.gene04068.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG