AlfalfaGEDB | MS.gene048746

M. sativa cultivar XinJiangDaYe / MS.gene048746

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene048746.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene048746.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene048746.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene04067	MS.gene048746	PPI
MS.gene71215	MS.gene048746	PPI
MS.gene061870	MS.gene048746	PPI
MS.gene035718	MS.gene048746	PPI
MS.gene048746	MS.gene016624	PPI
MS.gene048746	MS.gene008276	PPI
MS.gene66225	MS.gene048746	PPI
MS.gene071136	MS.gene048746	PPI
MS.gene048746	MS.gene73136	PPI
MS.gene048746	MS.gene049821	PPI
MS.gene048746	MS.gene04068	PPI
MS.gene048746	MS.gene05623	PPI
MS.gene78578	MS.gene048746	PPI
MS.gene79195	MS.gene048746	PPI
MS.gene035721	MS.gene048746	PPI
MS.gene048746	MS.gene035720	PPI
MS.gene048746	MS.gene61017	PPI
MS.gene064557	MS.gene048746	PPI
MS.gene038469	MS.gene048746	PPI
MS.gene048746	MS.gene09871	PPI
MS.gene04433	MS.gene048746	PPI
MS.gene04452	MS.gene048746	PPI
MS.gene048746	MS.gene049705	PPI
MS.gene048746	MS.gene04434	PPI
MS.gene048746	MS.gene070907	PPI
MS.gene048746	MS.gene04392	PPI
MS.gene035787	MS.gene048746	PPI
MS.gene048746	MS.gene010219	PPI
MS.gene048746	MS.gene05608	PPI
MS.gene89487	MS.gene048746	PPI
MS.gene048746	MS.gene68545	PPI
MS.gene035719	MS.gene048746	PPI
MS.gene58854	MS.gene048746	PPI
MS.gene70955	MS.gene048746	PPI
MS.gene048746	MS.gene82546	PPI
MS.gene040383	MS.gene048746	PPI
MS.gene070242	MS.gene048746	PPI
MS.gene072493	MS.gene048746	PPI
MS.gene30920	MS.gene048746	PPI
MS.gene010220	MS.gene048746	PPI
MS.gene08053	MS.gene048746	PPI
MS.gene061872	MS.gene048746	PPI
MS.gene048746	MS.gene09873	PPI
MS.gene88680	MS.gene048746	PPI
MS.gene070241	MS.gene048746	PPI
MS.gene056215	MS.gene048746	PPI
MS.gene022946	MS.gene048746	PPI
MS.gene048746	MS.gene62520	PPI
MS.gene061876	MS.gene048746	PPI
MS.gene048746	MS.gene035897	PPI
MS.gene012391	MS.gene048746	PPI
MS.gene09871	MS.gene048746	PPI
MS.gene035897	MS.gene048746	PPI
MS.gene060463	MS.gene048746	PPI
MS.gene048746	MS.gene84536	PPI
MS.gene04392	MS.gene048746	PPI
MS.gene048746	MS.gene64650	PPI
MS.gene048746	MS.gene60093	PPI
MS.gene04068	MS.gene048746	PPI
MS.gene048746	MS.gene08122	PPI
MS.gene048746	MS.gene061869	PPI
MS.gene04434	MS.gene048746	PPI
MS.gene048746	MS.gene04972	PPI
MS.gene048746	MS.gene004121	PPI
MS.gene08121	MS.gene048746	PPI
MS.gene022932	MS.gene048746	PPI
MS.gene048746	MS.gene018578	PPI
MS.gene88681	MS.gene048746	PPI
MS.gene048746	MS.gene061867	PPI
MS.gene070244	MS.gene048746	PPI
MS.gene048746	MS.gene04064	PPI
MS.gene048746	MS.gene018580	PPI
MS.gene035896	MS.gene048746	PPI
MS.gene64920	MS.gene048746	PPI
MS.gene048746	MS.gene004124	PPI
MS.gene048746	MS.gene05619	PPI
MS.gene009027	MS.gene048746	PPI
MS.gene31196	MS.gene048746	PPI
MS.gene048746	MS.gene04052	PPI
MS.gene048746	MS.gene89488	PPI
MS.gene048746	MS.gene061866	PPI
MS.gene060465	MS.gene048746	PPI
MS.gene05623	MS.gene048746	PPI
MS.gene043967	MS.gene048746	PPI
MS.gene048746	MS.gene79195	PPI
MS.gene048746	MS.gene048705	PPI
MS.gene048746	MS.gene061874	PPI
MS.gene048746	MS.gene04576	PPI
MS.gene028629	MS.gene048746	PPI
MS.gene048746	MS.gene012390	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene048746.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048746.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048746.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048746.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048746.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048746.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048746.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048746.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048746.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048746.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048746.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048746.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048746.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048746.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048746.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene048746.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene048746.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene048746.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene048746.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene048746.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene048746.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene048746.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene048746.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene048746.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene048746.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene048746.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene048746.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 36 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCGCCAATCGACGAATTGC+AGG	0.267346	3.4:+66821067	None:intergenic
GGAAGAACCCTCTATGGATT+TGG	0.370047	3.4:-66820881	MS.gene048746:CDS
AGACAAGGAAGAACCCTCTA+TGG	0.396310	3.4:-66820887	MS.gene048746:CDS
AGGTGTCTTGAAGATCTTCT+TGG	0.405705	3.4:-66820996	MS.gene048746:CDS
GGGAGGTAAGGGACTTGGAA+AGG	0.449159	3.4:-66821146	MS.gene048746:CDS
GGAGGTAAGGGACTTGGAAA+GGG	0.467307	3.4:-66821145	MS.gene048746:CDS
GGAAAGGGAGGTAAGGGACT+TGG	0.470633	3.4:-66821151	MS.gene048746:CDS
TTCAACCTCCAAATCCATAG+AGG	0.476286	3.4:+66820873	None:intergenic
CTGTTGAAAATGTCAGGTCG+TGG	0.487154	3.4:-66821172	None:intergenic
CAATCGACGAATTGCAGGCT+TGG	0.491738	3.4:+66821072	None:intergenic
AGGTCGTGGAAAGGGAGGTA+AGG	0.492819	3.4:-66821158	MS.gene048746:CDS
AGTTCTCCGCGATAACATCC+AGG	0.504812	3.4:-66821101	MS.gene048746:CDS
AGAACCCTCTATGGATTTGG+AGG	0.508817	3.4:-66820878	MS.gene048746:CDS
GAAGATCTTCAAGACACCTC+TGG	0.510902	3.4:+66821000	None:intergenic
CAAGCCTGCAATTCGTCGAT+TGG	0.511374	3.4:-66821071	MS.gene048746:CDS
TTACATACACTGAGCATGCT+AGG	0.520723	3.4:-66820952	MS.gene048746:CDS
GAGGAAGACGGTGACAGCCA+TGG	0.546349	3.4:-66820930	MS.gene048746:CDS
AGGGAGGTGCAAAGAGGCAT+AGG	0.547891	3.4:-66821126	MS.gene048746:CDS
AAAATGTCAGGTCGTGGAAA+GGG	0.548959	3.4:-66821166	MS.gene048746:CDS
GGCGGAGTTAAGAGAATCAG+TGG	0.555739	3.4:-66821040	MS.gene048746:CDS
GGTAAGGGACTTGGAAAGGG+AGG	0.560743	3.4:-66821142	MS.gene048746:CDS
GGTCGTGGAAAGGGAGGTAA+GGG	0.564245	3.4:-66821157	MS.gene048746:CDS
GTCTATGCTTTGAAGAGACA+AGG	0.570048	3.4:-66820902	MS.gene048746:CDS
CGTCGATTGGCGAGAAGAGG+CGG	0.584174	3.4:-66821058	MS.gene048746:CDS
GAAAATGTCAGGTCGTGGAA+AGG	0.584382	3.4:-66821167	None:intergenic
ATTCGTCGATTGGCGAGAAG+AGG	0.604517	3.4:-66821061	MS.gene048746:CDS
TTGCAGGCTTGGTGATACCC+TGG	0.613296	3.4:+66821083	None:intergenic
CAAAGCATAGACAACATCCA+TGG	0.629842	3.4:+66820913	None:intergenic
TCAACCTCCAAATCCATAGA+GGG	0.633476	3.4:+66820874	None:intergenic
ATGTCAGGTCGTGGAAAGGG+AGG	0.643397	3.4:-66821163	MS.gene048746:CDS
TGATACCCTGGATGTTATCG+CGG	0.665173	3.4:+66821095	None:intergenic
TGAGCATGCTAGGAGGAAGA+CGG	0.671956	3.4:-66820942	MS.gene048746:CDS
GTTCTCCGCGATAACATCCA+GGG	0.672360	3.4:-66821100	MS.gene048746:CDS
TTGGAAAGGGAGGTGCAAAG+AGG	0.683014	3.4:-66821132	MS.gene048746:CDS
TTGATCTATGAAGAAACCAG+AGG	0.704889	3.4:-66821016	MS.gene048746:CDS
CATACACTGAGCATGCTAGG+AGG	0.707859	3.4:-66820949	MS.gene048746:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGATCTATGAAGAAACCAG+AGG	-	chr3.4:66821021-66821040	MS.gene048746:CDS	35.0%
	AGGTGTCTTGAAGATCTTCT+TGG	-	chr3.4:66821041-66821060	MS.gene048746:CDS	40.0%
	CAAAGCATAGACAACATCCA+TGG	+	chr3.4:66821127-66821146	None:intergenic	40.0%
	TCAACCTCCAAATCCATAGA+GGG	+	chr3.4:66821166-66821185	None:intergenic	40.0%
	TTACATACACTGAGCATGCT+AGG	-	chr3.4:66821085-66821104	MS.gene048746:CDS	40.0%
!!	GTCTATGCTTTGAAGAGACA+AGG	-	chr3.4:66821135-66821154	MS.gene048746:CDS	40.0%
	AGAACCCTCTATGGATTTGG+AGG	-	chr3.4:66821159-66821178	MS.gene048746:CDS	45.0%
	AGACAAGGAAGAACCCTCTA+TGG	-	chr3.4:66821150-66821169	MS.gene048746:CDS	45.0%
	GAAGATCTTCAAGACACCTC+TGG	+	chr3.4:66821040-66821059	None:intergenic	45.0%
	GGAAGAACCCTCTATGGATT+TGG	-	chr3.4:66821156-66821175	MS.gene048746:CDS	45.0%
	TGATACCCTGGATGTTATCG+CGG	+	chr3.4:66820945-66820964	None:intergenic	45.0%
	AGTTCTCCGCGATAACATCC+AGG	-	chr3.4:66820936-66820955	MS.gene048746:CDS	50.0%
	CAAGCCTGCAATTCGTCGAT+TGG	-	chr3.4:66820966-66820985	MS.gene048746:CDS	50.0%
	CAATCGACGAATTGCAGGCT+TGG	+	chr3.4:66820968-66820987	None:intergenic	50.0%
	CATACACTGAGCATGCTAGG+AGG	-	chr3.4:66821088-66821107	MS.gene048746:CDS	50.0%
	GGAGGTAAGGGACTTGGAAA+GGG	-	chr3.4:66820892-66820911	MS.gene048746:CDS	50.0%
	GGCGGAGTTAAGAGAATCAG+TGG	-	chr3.4:66820997-66821016	MS.gene048746:CDS	50.0%
	GTTCTCCGCGATAACATCCA+GGG	-	chr3.4:66820937-66820956	MS.gene048746:CDS	50.0%
	TGAGCATGCTAGGAGGAAGA+CGG	-	chr3.4:66821095-66821114	MS.gene048746:CDS	50.0%
!	ATTCGTCGATTGGCGAGAAG+AGG	-	chr3.4:66820976-66820995	MS.gene048746:CDS	50.0%
!	TTGGAAAGGGAGGTGCAAAG+AGG	-	chr3.4:66820905-66820924	MS.gene048746:CDS	50.0%
	AGGTCGTGGAAAGGGAGGTA+AGG	-	chr3.4:66820879-66820898	MS.gene048746:CDS	55.0%
	ATGTCAGGTCGTGGAAAGGG+AGG	-	chr3.4:66820874-66820893	MS.gene048746:CDS	55.0%
	CTCGCCAATCGACGAATTGC+AGG	+	chr3.4:66820973-66820992	None:intergenic	55.0%
	GGAAAGGGAGGTAAGGGACT+TGG	-	chr3.4:66820886-66820905	MS.gene048746:CDS	55.0%
	GGGAGGTAAGGGACTTGGAA+AGG	-	chr3.4:66820891-66820910	MS.gene048746:CDS	55.0%
	GGTAAGGGACTTGGAAAGGG+AGG	-	chr3.4:66820895-66820914	MS.gene048746:CDS	55.0%
	GGTCGTGGAAAGGGAGGTAA+GGG	-	chr3.4:66820880-66820899	MS.gene048746:CDS	55.0%
!	AGGGAGGTGCAAAGAGGCAT+AGG	-	chr3.4:66820911-66820930	MS.gene048746:CDS	55.0%
!!	TTGCAGGCTTGGTGATACCC+TGG	+	chr3.4:66820957-66820976	None:intergenic	55.0%
	GAGGAAGACGGTGACAGCCA+TGG	-	chr3.4:66821107-66821126	MS.gene048746:CDS	60.0%
!	CGTCGATTGGCGAGAAGAGG+CGG	-	chr3.4:66820979-66820998	MS.gene048746:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	66820874	66821185	66820874	ID=MS.gene048746
chr3.4	mRNA	66820874	66821185	66820874	ID=MS.gene048746.t1;Parent=MS.gene048746
chr3.4	exon	66820874	66821185	66820874	ID=MS.gene048746.t1.exon1;Parent=MS.gene048746.t1
chr3.4	CDS	66820874	66821185	66820874	ID=cds.MS.gene048746.t1;Parent=MS.gene048746.t1

Gene Sequence	>MS.gene048746 ATGTCAGGTCGTGGAAAGGGAGGTAAGGGACTTGGAAAGGGAGGTGCAAAGAGGCATAGGAAAGTTCTCCGCGATAACATCCAGGGTATCACCAAGCCTGCAATTCGTCGATTGGCGAGAAGAGGCGGAGTTAAGAGAATCAGTGGATTGATCTATGAAGAAACCAGAGGTGTCTTGAAGATCTTCTTGGAAAACGTCATTCGTGATGCAGTTACATACACTGAGCATGCTAGGAGGAAGACGGTGACAGCCATGGATGTTGTCTATGCTTTGAAGAGACAAGGAAGAACCCTCTATGGATTTGGAGGTTGA
Protein sequence	>MS.gene048746.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG