AlfalfaGEDB | MS.gene08121

M. sativa cultivar XinJiangDaYe / MS.gene08121

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08121.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08121.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08121.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene08121	MS.gene84536	PPI
MS.gene08121	MS.gene035896	PPI
MS.gene08121	MS.gene82546	PPI
MS.gene88680	MS.gene08121	PPI
MS.gene056215	MS.gene08121	PPI
MS.gene064557	MS.gene08121	PPI
MS.gene08121	MS.gene60093	PPI
MS.gene038469	MS.gene08121	PPI
MS.gene08121	MS.gene08122	PPI
MS.gene08121	MS.gene09873	PPI
MS.gene08121	MS.gene022932	PPI
MS.gene04452	MS.gene08121	PPI
MS.gene08121	MS.gene010220	PPI
MS.gene08121	MS.gene04972	PPI
MS.gene035787	MS.gene08121	PPI
MS.gene08121	MS.gene004121	PPI
MS.gene08121	MS.gene62520	PPI
MS.gene08121	MS.gene018578	PPI
MS.gene89487	MS.gene08121	PPI
MS.gene08121	MS.gene035721	PPI
MS.gene08121	MS.gene04064	PPI
MS.gene08121	MS.gene018580	PPI
MS.gene08121	MS.gene004124	PPI
MS.gene30920	MS.gene08121	PPI
MS.gene08121	MS.gene072493	PPI
MS.gene08053	MS.gene08121	PPI
MS.gene061872	MS.gene08121	PPI
MS.gene08121	MS.gene04052	PPI
MS.gene08121	MS.gene061866	PPI
MS.gene070241	MS.gene08121	PPI
MS.gene08121	MS.gene64650	PPI
MS.gene08121	MS.gene79195	PPI
MS.gene08121	MS.gene048705	PPI
MS.gene08121	MS.gene061874	PPI
MS.gene08121	MS.gene04576	PPI
MS.gene08121	MS.gene061869	PPI
MS.gene061876	MS.gene08121	PPI
MS.gene012391	MS.gene08121	PPI
MS.gene88681	MS.gene08121	PPI
MS.gene08121	MS.gene04452	PPI
MS.gene070244	MS.gene08121	PPI
MS.gene64920	MS.gene08121	PPI
MS.gene060463	MS.gene08121	PPI
MS.gene08121	MS.gene061867	PPI
MS.gene08121	MS.gene008276	PPI
MS.gene31196	MS.gene08121	PPI
MS.gene08121	MS.gene05619	PPI
MS.gene08121	MS.gene73136	PPI
MS.gene060465	MS.gene08121	PPI
MS.gene08121	MS.gene049821	PPI
MS.gene08121	MS.gene04068	PPI
MS.gene08121	MS.gene022946	PPI
MS.gene08121	MS.gene05623	PPI
MS.gene08121	MS.gene89488	PPI
MS.gene08121	MS.gene66225	PPI
MS.gene08121	MS.gene035720	PPI
MS.gene08121	MS.gene70955	PPI
MS.gene08121	MS.gene61017	PPI
MS.gene08121	MS.gene09871	PPI
MS.gene08121	MS.gene012390	PPI
MS.gene08121	MS.gene064557	PPI
MS.gene08121	MS.gene04434	PPI
MS.gene08121	MS.gene070907	PPI
MS.gene08121	MS.gene04392	PPI
MS.gene035718	MS.gene08121	PPI
MS.gene08121	MS.gene010219	PPI
MS.gene08121	MS.gene040383	PPI
MS.gene08121	MS.gene68545	PPI
MS.gene009027	MS.gene08121	PPI
MS.gene08121	MS.gene070242	PPI
MS.gene08121	MS.gene016624	PPI
MS.gene78578	MS.gene08121	PPI
MS.gene08121	MS.gene048746	PPI
MS.gene028629	MS.gene08121	PPI
MS.gene04433	MS.gene08121	PPI
MS.gene08121	MS.gene043967	PPI
MS.gene04067	MS.gene08121	PPI
MS.gene71215	MS.gene08121	PPI
MS.gene061870	MS.gene08121	PPI
MS.gene08121	MS.gene035897	PPI
MS.gene08121	MS.gene049705	PPI
MS.gene035719	MS.gene08121	PPI
MS.gene58854	MS.gene08121	PPI
MS.gene08121	MS.gene05608	PPI
MS.gene70955	MS.gene08121	PPI
MS.gene071136	MS.gene08121	PPI
MS.gene040383	MS.gene08121	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08121.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08121.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08121.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08121.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08121.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08121.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08121.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08121.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08121.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08121.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08121.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08121.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08121.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08121.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08121.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene08121.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08121.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08121.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08121.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08121.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08121.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08121.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08121.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08121.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08121.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08121.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08121.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 37 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTTCTTCGTGATAACATTC+AGG	0.304269	3.3:-50018169	MS.gene08121:CDS
GGAAGAACCCTCTATGGATT+TGG	0.370047	3.3:-50017949	MS.gene08121:CDS
AGACAAGGAAGAACCCTCTA+TGG	0.374939	3.3:-50017955	MS.gene08121:CDS
GGAAAGGGAGGTAAGGGATT+AGG	0.413383	3.3:-50018219	MS.gene08121:CDS
AGGTGTATTGAAGATCTTCT+TGG	0.432837	3.3:-50018064	MS.gene08121:CDS
TTCAACCTCCAAATCCATAG+AGG	0.435462	3.3:+50017941	None:intergenic
CAATCGACGAATAGCAGGTT+TGG	0.439411	3.3:+50018140	None:intergenic
GGTAAGGGATTAGGAAAGGG+AGG	0.470949	3.3:-50018210	MS.gene08121:CDS
CTTGCCAATCGACGAATAGC+AGG	0.487494	3.3:+50018135	None:intergenic
AGGCCGTGGAAAGGGAGGTA+AGG	0.492819	3.3:-50018226	MS.gene08121:CDS
ATCCCTTACCTCCCTTTCCA+CGG	0.504513	3.3:+50018223	None:intergenic
GAAGATCTTCAATACACCTC+TGG	0.507768	3.3:+50018068	None:intergenic
AGAACCCTCTATGGATTTGG+AGG	0.508817	3.3:-50017946	MS.gene08121:CDS
GTTCTTCGTGATAACATTCA+GGG	0.509125	3.3:-50018168	MS.gene08121:CDS
CTCGCGAAAATGTCAGGCCG+TGG	0.510433	3.3:-50018240	None:intergenic
GGAGGTAAGGGATTAGGAAA+GGG	0.512610	3.3:-50018213	MS.gene08121:CDS
AAAATGTCAGGCCGTGGAAA+GGG	0.516096	3.3:-50018234	MS.gene08121:CDS
GGGAGGTAAGGGATTAGGAA+AGG	0.522188	3.3:-50018214	MS.gene08121:CDS
AGGTGCAAAGAGGCATAGAA+AGG	0.530485	3.3:-50018190	MS.gene08121:CDS
GGCCGTGGAAAGGGAGGTAA+GGG	0.552535	3.3:-50018225	MS.gene08121:CDS
CAAACCTGCTATTCGTCGAT+TGG	0.553041	3.3:-50018139	MS.gene08121:CDS
GAAAATGTCAGGCCGTGGAA+AGG	0.558967	3.3:-50018235	None:intergenic
GAGGAAGACGGTGACAGCCA+TGG	0.561434	3.3:-50017998	MS.gene08121:CDS
TGACATACACTGAGCATGCA+AGG	0.575443	3.3:-50018020	MS.gene08121:CDS
CGTCGATTGGCAAGAAGAGG+CGG	0.578876	3.3:-50018126	MS.gene08121:CDS
GTCTATGCTTTAAAGAGACA+AGG	0.587268	3.3:-50017970	MS.gene08121:CDS
GGCGGTGTCAAGAGAATCAG+TGG	0.591241	3.3:-50018108	MS.gene08121:CDS
TCAACCTCCAAATCCATAGA+GGG	0.597347	3.3:+50017942	None:intergenic
AATCAGTGGATTGATCTATG+AGG	0.599604	3.3:-50018094	MS.gene08121:CDS
ATTCGTCGATTGGCAAGAAG+AGG	0.622105	3.3:-50018129	MS.gene08121:CDS
CAGTGTATGTCACAGCGTCA+CGG	0.632042	3.3:+50018031	None:intergenic
ATGTCAGGCCGTGGAAAGGG+AGG	0.634999	3.3:-50018231	MS.gene08121:CDS
TAAAGCATAGACAACATCCA+TGG	0.638346	3.3:+50017981	None:intergenic
TGAGCATGCAAGGAGGAAGA+CGG	0.650199	3.3:-50018010	MS.gene08121:CDS
TAGGAAAGGGAGGTGCAAAG+AGG	0.683932	3.3:-50018200	MS.gene08121:CDS
TTGATCTATGAGGAAACCAG+AGG	0.700822	3.3:-50018084	MS.gene08121:CDS
CATACACTGAGCATGCAAGG+AGG	0.756152	3.3:-50018017	MS.gene08121:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGGTGTATTGAAGATCTTCT+TGG	-	chr3.3:50018109-50018128	MS.gene08121:CDS	35.0%
	GTTCTTCGTGATAACATTCA+GGG	-	chr3.3:50018005-50018024	MS.gene08121:CDS	35.0%
	TAAAGCATAGACAACATCCA+TGG	+	chr3.3:50018195-50018214	None:intergenic	35.0%
!!	AATCAGTGGATTGATCTATG+AGG	-	chr3.3:50018079-50018098	MS.gene08121:CDS	35.0%
!!	GTCTATGCTTTAAAGAGACA+AGG	-	chr3.3:50018203-50018222	MS.gene08121:CDS	35.0%
	GAAGATCTTCAATACACCTC+TGG	+	chr3.3:50018108-50018127	None:intergenic	40.0%
	GGTTCTTCGTGATAACATTC+AGG	-	chr3.3:50018004-50018023	MS.gene08121:CDS	40.0%
	TCAACCTCCAAATCCATAGA+GGG	+	chr3.3:50018234-50018253	None:intergenic	40.0%
	TTGATCTATGAGGAAACCAG+AGG	-	chr3.3:50018089-50018108	MS.gene08121:CDS	40.0%
	AGAACCCTCTATGGATTTGG+AGG	-	chr3.3:50018227-50018246	MS.gene08121:CDS	45.0%
	AGACAAGGAAGAACCCTCTA+TGG	-	chr3.3:50018218-50018237	MS.gene08121:CDS	45.0%
	CAAACCTGCTATTCGTCGAT+TGG	-	chr3.3:50018034-50018053	MS.gene08121:CDS	45.0%
	CAATCGACGAATAGCAGGTT+TGG	+	chr3.3:50018036-50018055	None:intergenic	45.0%
	GGAAGAACCCTCTATGGATT+TGG	-	chr3.3:50018224-50018243	MS.gene08121:CDS	45.0%
	GGAGGTAAGGGATTAGGAAA+GGG	-	chr3.3:50017960-50017979	MS.gene08121:CDS	45.0%
	TGACATACACTGAGCATGCA+AGG	-	chr3.3:50018153-50018172	MS.gene08121:CDS	45.0%
!	AGGTGCAAAGAGGCATAGAA+AGG	-	chr3.3:50017983-50018002	MS.gene08121:CDS	45.0%
!	ATTCGTCGATTGGCAAGAAG+AGG	-	chr3.3:50018044-50018063	MS.gene08121:CDS	45.0%
	ATCCCTTACCTCCCTTTCCA+CGG	+	chr3.3:50017953-50017972	None:intergenic	50.0%
	CAGTGTATGTCACAGCGTCA+CGG	+	chr3.3:50018145-50018164	None:intergenic	50.0%
	CATACACTGAGCATGCAAGG+AGG	-	chr3.3:50018156-50018175	MS.gene08121:CDS	50.0%
	CTTGCCAATCGACGAATAGC+AGG	+	chr3.3:50018041-50018060	None:intergenic	50.0%
	GGAAAGGGAGGTAAGGGATT+AGG	-	chr3.3:50017954-50017973	MS.gene08121:CDS	50.0%
	GGGAGGTAAGGGATTAGGAA+AGG	-	chr3.3:50017959-50017978	MS.gene08121:CDS	50.0%
	GGTAAGGGATTAGGAAAGGG+AGG	-	chr3.3:50017963-50017982	MS.gene08121:CDS	50.0%
	TGAGCATGCAAGGAGGAAGA+CGG	-	chr3.3:50018163-50018182	MS.gene08121:CDS	50.0%
!	TAGGAAAGGGAGGTGCAAAG+AGG	-	chr3.3:50017973-50017992	MS.gene08121:CDS	50.0%
!	CGTCGATTGGCAAGAAGAGG+CGG	-	chr3.3:50018047-50018066	MS.gene08121:CDS	55.0%
!	GGCGGTGTCAAGAGAATCAG+TGG	-	chr3.3:50018065-50018084	MS.gene08121:CDS	55.0%
	AGGCCGTGGAAAGGGAGGTA+AGG	-	chr3.3:50017947-50017966	MS.gene08121:CDS	60.0%
	ATGTCAGGCCGTGGAAAGGG+AGG	-	chr3.3:50017942-50017961	MS.gene08121:CDS	60.0%
	GAGGAAGACGGTGACAGCCA+TGG	-	chr3.3:50018175-50018194	MS.gene08121:CDS	60.0%
	GGCCGTGGAAAGGGAGGTAA+GGG	-	chr3.3:50017948-50017967	MS.gene08121:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	50017942	50018253	50017942	ID=MS.gene08121
chr3.3	mRNA	50017942	50018253	50017942	ID=MS.gene08121.t1;Parent=MS.gene08121
chr3.3	exon	50017942	50018253	50017942	ID=MS.gene08121.t1.exon1;Parent=MS.gene08121.t1
chr3.3	CDS	50017942	50018253	50017942	ID=cds.MS.gene08121.t1;Parent=MS.gene08121.t1

Gene Sequence	>MS.gene08121 ATGTCAGGCCGTGGAAAGGGAGGTAAGGGATTAGGAAAGGGAGGTGCAAAGAGGCATAGAAAGGTTCTTCGTGATAACATTCAGGGTATCACCAAACCTGCTATTCGTCGATTGGCAAGAAGAGGCGGTGTCAAGAGAATCAGTGGATTGATCTATGAGGAAACCAGAGGTGTATTGAAGATCTTCTTGGAGAATGTCATCCGTGACGCTGTGACATACACTGAGCATGCAAGGAGGAAGACGGTGACAGCCATGGATGTTGTCTATGCTTTAAAGAGACAAGGAAGAACCCTCTATGGATTTGGAGGTTGA
Protein sequence	>MS.gene08121.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG