AlfalfaGEDB | MS.gene012391

M. sativa cultivar XinJiangDaYe / MS.gene012391

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene012391.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene012391.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene012391.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene035787	MS.gene012391	PPI
MS.gene012391	MS.gene072493	PPI
MS.gene012391	MS.gene04052	PPI
MS.gene012391	MS.gene84536	PPI
MS.gene012391	MS.gene035896	PPI
MS.gene012391	MS.gene061866	PPI
MS.gene012391	MS.gene64650	PPI
MS.gene012391	MS.gene79195	PPI
MS.gene012391	MS.gene048705	PPI
MS.gene012391	MS.gene061876	PPI
MS.gene012391	MS.gene061874	PPI
MS.gene012391	MS.gene056215	PPI
MS.gene012391	MS.gene061869	PPI
MS.gene012391	MS.gene04452	PPI
MS.gene012391	MS.gene070244	PPI
MS.gene012391	MS.gene004121	PPI
MS.gene070241	MS.gene012391	PPI
MS.gene012391	MS.gene009027	PPI
MS.gene012391	MS.gene038469	PPI
MS.gene012391	MS.gene061867	PPI
MS.gene012391	MS.gene04064	PPI
MS.gene061876	MS.gene012391	PPI
MS.gene012391	MS.gene008276	PPI
MS.gene012391	MS.gene30920	PPI
MS.gene012391	MS.gene05619	PPI
MS.gene012391	MS.gene73136	PPI
MS.gene012391	MS.gene049821	PPI
MS.gene012391	MS.gene78578	PPI
MS.gene012391	MS.gene04068	PPI
MS.gene012391	MS.gene022946	PPI
MS.gene060463	MS.gene012391	PPI
MS.gene012391	MS.gene035719	PPI
MS.gene012391	MS.gene05623	PPI
MS.gene012391	MS.gene061872	PPI
MS.gene012391	MS.gene89488	PPI
MS.gene012391	MS.gene66225	PPI
MS.gene012391	MS.gene035720	PPI
MS.gene012391	MS.gene70955	PPI
MS.gene012391	MS.gene61017	PPI
MS.gene012391	MS.gene04576	PPI
MS.gene012391	MS.gene09871	PPI
MS.gene012391	MS.gene012390	PPI
MS.gene012391	MS.gene08121	PPI
MS.gene012391	MS.gene64920	PPI
MS.gene012391	MS.gene04067	PPI
MS.gene012391	MS.gene064557	PPI
MS.gene012391	MS.gene035787	PPI
MS.gene012391	MS.gene04434	PPI
MS.gene012391	MS.gene04392	PPI
MS.gene012391	MS.gene060463	PPI
MS.gene012391	MS.gene89487	PPI
MS.gene012391	MS.gene010219	PPI
MS.gene012391	MS.gene04433	PPI
MS.gene012391	MS.gene040383	PPI
MS.gene012391	MS.gene68545	PPI
MS.gene012391	MS.gene070242	PPI
MS.gene012391	MS.gene016624	PPI
MS.gene012391	MS.gene048746	PPI
MS.gene012391	MS.gene028629	PPI
MS.gene012391	MS.gene58854	PPI
MS.gene78578	MS.gene012391	PPI
MS.gene012391	MS.gene043967	PPI
MS.gene012391	MS.gene31196	PPI
MS.gene012391	MS.gene060465	PPI
MS.gene012391	MS.gene035897	PPI
MS.gene012391	MS.gene049705	PPI
MS.gene012391	MS.gene061870	PPI
MS.gene012391	MS.gene070907	PPI
MS.gene012391	MS.gene05608	PPI
MS.gene04067	MS.gene012391	PPI
MS.gene71215	MS.gene012391	PPI
MS.gene012391	MS.gene070241	PPI
MS.gene012391	MS.gene88681	PPI
MS.gene012391	MS.gene82546	PPI
MS.gene012391	MS.gene60093	PPI
MS.gene012391	MS.gene71215	PPI
MS.gene012391	MS.gene08122	PPI
MS.gene071136	MS.gene012391	PPI
MS.gene012391	MS.gene09873	PPI
MS.gene012391	MS.gene035718	PPI
MS.gene012391	MS.gene022932	PPI
MS.gene012391	MS.gene010220	PPI
MS.gene012391	MS.gene04972	PPI
MS.gene056215	MS.gene012391	PPI
MS.gene012391	MS.gene62520	PPI
MS.gene012391	MS.gene08053	PPI
MS.gene012391	MS.gene018578	PPI
MS.gene012391	MS.gene035721	PPI
MS.gene012391	MS.gene018580	PPI
MS.gene012391	MS.gene004124	PPI
MS.gene012391	MS.gene071136	PPI
MS.gene012391	MS.gene88680	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene012391.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012391.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012391.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012391.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012391.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012391.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012391.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012391.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012391.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012391.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012391.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012391.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012391.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012391.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012391.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene012391.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene012391.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene012391.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene012391.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene012391.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene012391.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene012391.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene012391.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene012391.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene012391.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene012391.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene012391.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 31 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGCAGGGAAGGACTCTTTA+TGG	0.247348	8.3:-3052374	MS.gene012391:CDS
GGAAGGACTCTTTATGGATT+TGG	0.297351	8.3:-3052368	MS.gene012391:CDS
TCCTTTGCCTCTTCCTGACA+TGG	0.418595	8.3:+3052652	None:intergenic
TCTTTATGGATTTGGCGGTT+AGG	0.439485	8.3:-3052360	None:intergenic
GGCAAAGGAGGAAAGGGTCT+TGG	0.446329	8.3:-3052638	MS.gene012391:CDS
CAAGAATTCACAGCCATGTC+AGG	0.481900	8.3:-3052665	None:intergenic
CGTTTACGCTCTCAAGAGGC+AGG	0.488613	8.3:-3052390	MS.gene012391:CDS
GGTTCTTCGCGATAACATCC+AGG	0.500175	8.3:-3052588	MS.gene012391:CDS
AGGACTCTTTATGGATTTGG+CGG	0.509079	8.3:-3052365	MS.gene012391:CDS
GGAGGAAAGGGTCTTGGAAA+GGG	0.511441	8.3:-3052632	MS.gene012391:CDS
TCGCAGGCTTCGTGATTCCC+TGG	0.511861	8.3:+3052570	None:intergenic
AGGAAGAGGCAAAGGAGGAA+AGG	0.518412	8.3:-3052645	MS.gene012391:CDS
GGAAGAGGCAAAGGAGGAAA+GGG	0.527098	8.3:-3052644	MS.gene012391:CDS
GCCATGTCAGGAAGAGGCAA+AGG	0.547501	8.3:-3052653	MS.gene012391:CDS
CCGTAAAACTGTTACTGCGA+TGG	0.549023	8.3:-3052417	MS.gene012391:CDS
AGGAGGAAAGGGTCTTGGAA+AGG	0.553664	8.3:-3052633	MS.gene012391:CDS
ACGGCGAGCATGCTCTGTGT+AGG	0.562056	8.3:+3052436	None:intergenic
TTCACAGCCATGTCAGGAAG+AGG	0.573281	8.3:-3052659	None:intergenic
AGGAGCAAAACGACACCGTA+AGG	0.575994	8.3:-3052609	MS.gene012391:CDS
TGTTATCGCGAAGAACCTTA+CGG	0.576355	8.3:+3052594	None:intergenic
ATGTCAGGAAGAGGCAAAGG+AGG	0.581057	8.3:-3052650	MS.gene012391:CDS
ACGCTCTCAAGAGGCAGGGA+AGG	0.583359	8.3:-3052385	MS.gene012391:CDS
CTTGCAAGACGACGAATCGC+AGG	0.588436	8.3:+3052554	None:intergenic
GGAAAGGGTCTTGGAAAGGG+AGG	0.621886	8.3:-3052629	MS.gene012391:CDS
CGTCGTCTTGCAAGACGTGG+TGG	0.633046	8.3:-3052545	MS.gene012391:CDS
TTAATCTATGAAGAAACTCG+TGG	0.634992	8.3:-3052503	MS.gene012391:CDS
ATGTCGTTTACGCTCTCAAG+AGG	0.636714	8.3:-3052394	MS.gene012391:CDS
ATTCGTCGTCTTGCAAGACG+TGG	0.646636	8.3:-3052548	MS.gene012391:CDS
GTTCTTCGCGATAACATCCA+GGG	0.657095	8.3:-3052587	MS.gene012391:CDS
GTTTACGCTCTCAAGAGGCA+GGG	0.663401	8.3:-3052389	MS.gene012391:CDS
GGTGGTGTGAAGCGTATCAG+TGG	0.669421	8.3:-3052527	MS.gene012391:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTAATCTATGAAGAAACTCG+TGG	-	chr8.3:3052508-3052527	MS.gene012391:CDS	30.0%
	AGGACTCTTTATGGATTTGG+CGG	-	chr8.3:3052646-3052665	MS.gene012391:CDS	40.0%
	GGAAGGACTCTTTATGGATT+TGG	-	chr8.3:3052643-3052662	MS.gene012391:CDS	40.0%
	TGTTATCGCGAAGAACCTTA+CGG	+	chr8.3:3052420-3052439	None:intergenic	40.0%
!	CCATCGCAGTAACAGTTTTA+CGG	+	chr8.3:3052597-3052616	None:intergenic	40.0%
	CCGTAAAACTGTTACTGCGA+TGG	-	chr8.3:3052594-3052613	MS.gene012391:CDS	45.0%
	GTTCTTCGCGATAACATCCA+GGG	-	chr8.3:3052424-3052443	MS.gene012391:CDS	45.0%
!	ATGTCGTTTACGCTCTCAAG+AGG	-	chr8.3:3052617-3052636	MS.gene012391:CDS	45.0%
	AGGAAGAGGCAAAGGAGGAA+AGG	-	chr8.3:3052366-3052385	MS.gene012391:CDS	50.0%
	AGGAGCAAAACGACACCGTA+AGG	-	chr8.3:3052402-3052421	MS.gene012391:CDS	50.0%
	AGGAGGAAAGGGTCTTGGAA+AGG	-	chr8.3:3052378-3052397	MS.gene012391:CDS	50.0%
	AGGCAGGGAAGGACTCTTTA+TGG	-	chr8.3:3052637-3052656	MS.gene012391:CDS	50.0%
	ATGTCAGGAAGAGGCAAAGG+AGG	-	chr8.3:3052361-3052380	MS.gene012391:CDS	50.0%
	ATTCGTCGTCTTGCAAGACG+TGG	-	chr8.3:3052463-3052482	MS.gene012391:CDS	50.0%
	GGAAGAGGCAAAGGAGGAAA+GGG	-	chr8.3:3052367-3052386	MS.gene012391:CDS	50.0%
	GGAGGAAAGGGTCTTGGAAA+GGG	-	chr8.3:3052379-3052398	MS.gene012391:CDS	50.0%
	GGTTCTTCGCGATAACATCC+AGG	-	chr8.3:3052423-3052442	MS.gene012391:CDS	50.0%
	GTTTACGCTCTCAAGAGGCA+GGG	-	chr8.3:3052622-3052641	MS.gene012391:CDS	50.0%
	CGTTTACGCTCTCAAGAGGC+AGG	-	chr8.3:3052621-3052640	MS.gene012391:CDS	55.0%
	CTTGCAAGACGACGAATCGC+AGG	+	chr8.3:3052460-3052479	None:intergenic	55.0%
	GGAAAGGGTCTTGGAAAGGG+AGG	-	chr8.3:3052382-3052401	MS.gene012391:CDS	55.0%
	GGCAAAGGAGGAAAGGGTCT+TGG	-	chr8.3:3052373-3052392	MS.gene012391:CDS	55.0%
	GGTGGTGTGAAGCGTATCAG+TGG	-	chr8.3:3052484-3052503	MS.gene012391:CDS	55.0%
	ACGCTCTCAAGAGGCAGGGA+AGG	-	chr8.3:3052626-3052645	MS.gene012391:CDS	60.0%
	ACGGCGAGCATGCTCTGTGT+AGG	+	chr8.3:3052578-3052597	None:intergenic	60.0%
	CGTCGTCTTGCAAGACGTGG+TGG	-	chr8.3:3052466-3052485	MS.gene012391:CDS	60.0%
	TCGCAGGCTTCGTGATTCCC+TGG	+	chr8.3:3052444-3052463	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	3052361	3052672	3052361	ID=MS.gene012391
chr8.3	mRNA	3052361	3052672	3052361	ID=MS.gene012391.t1;Parent=MS.gene012391
chr8.3	exon	3052361	3052672	3052361	ID=MS.gene012391.t1.exon1;Parent=MS.gene012391.t1
chr8.3	CDS	3052361	3052672	3052361	ID=cds.MS.gene012391.t1;Parent=MS.gene012391.t1

Gene Sequence	>MS.gene012391 ATGTCAGGAAGAGGCAAAGGAGGAAAGGGTCTTGGAAAGGGAGGAGCAAAACGACACCGTAAGGTTCTTCGCGATAACATCCAGGGAATCACGAAGCCTGCGATTCGTCGTCTTGCAAGACGTGGTGGTGTGAAGCGTATCAGTGGCTTAATCTATGAAGAAACTCGTGGTGTTCTCAAAATCTTTCTTGAGAATGTGATTCGTGATGCTGTCACCTACACAGAGCATGCTCGCCGTAAAACTGTTACTGCGATGGATGTCGTTTACGCTCTCAAGAGGCAGGGAAGGACTCTTTATGGATTTGGCGGTTAG
Protein sequence	>MS.gene012391.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG