AlfalfaGEDB | MS.gene070241

M. sativa cultivar XinJiangDaYe / MS.gene070241

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070241.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070241.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070241.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene070241	0.828666	7.92E-55	-1.69E-46
MS.gene049159	MS.gene070241	0.826084	3.29E-54	-1.69E-46
MS.gene049255	MS.gene070241	0.800121	1.67E-48	-1.69E-46
MS.gene049341	MS.gene070241	0.802392	5.73E-49	-1.69E-46
MS.gene049821	MS.gene070241	0.904605	1.03E-79	-1.69E-46
MS.gene049850	MS.gene070241	0.817925	2.56E-52	-1.69E-46
MS.gene049887	MS.gene070241	0.805835	1.10E-49	-1.69E-46
MS.gene050020	MS.gene070241	0.822866	1.88E-53	-1.69E-46
MS.gene050442	MS.gene070241	0.812515	4.08E-51	-1.69E-46
MS.gene050689	MS.gene070241	0.829364	5.37E-55	-1.69E-46
MS.gene050807	MS.gene070241	0.833395	5.47E-56	-1.69E-46
MS.gene050808	MS.gene070241	0.80187	7.34E-49	-1.69E-46
MS.gene050815	MS.gene070241	0.829549	4.84E-55	-1.69E-46
MS.gene050838	MS.gene070241	0.832404	9.64E-56	-1.69E-46
MS.gene050901	MS.gene070241	0.839464	1.56E-57	-1.69E-46
MS.gene051243	MS.gene070241	0.800296	1.54E-48	-1.69E-46
MS.gene051247	MS.gene070241	0.838688	2.48E-57	-1.69E-46
MS.gene051328	MS.gene070241	0.847023	1.50E-59	-1.69E-46
MS.gene05157	MS.gene070241	0.813827	2.10E-51	-1.69E-46
MS.gene051700	MS.gene070241	0.82351	1.33E-53	-1.69E-46
MS.gene051719	MS.gene070241	0.823875	1.09E-53	-1.69E-46
MS.gene051720	MS.gene070241	0.823503	1.34E-53	-1.69E-46
MS.gene051975	MS.gene070241	0.810179	1.31E-50	-1.69E-46
MS.gene052133	MS.gene070241	0.860346	2.20E-63	-1.69E-46
MS.gene052335	MS.gene070241	0.804155	2.47E-49	-1.69E-46
MS.gene052516	MS.gene070241	0.838517	2.74E-57	-1.69E-46
MS.gene052665	MS.gene070241	0.822933	1.82E-53	-1.69E-46
MS.gene052896	MS.gene070241	0.805657	1.20E-49	-1.69E-46
MS.gene052947	MS.gene070241	0.819939	8.92E-53	-1.69E-46
MS.gene053008	MS.gene070241	0.818771	1.65E-52	-1.69E-46
MS.gene053226	MS.gene070241	0.812534	4.04E-51	-1.69E-46
MS.gene053563	MS.gene070241	0.808373	3.20E-50	-1.69E-46
MS.gene053581	MS.gene070241	0.850039	2.20E-60	-1.69E-46
MS.gene05364	MS.gene070241	0.821171	4.65E-53	-1.69E-46
MS.gene05462	MS.gene070241	0.814302	1.65E-51	-1.69E-46
MS.gene05517	MS.gene070241	0.807123	5.89E-50	-1.69E-46
MS.gene055465	MS.gene070241	0.849268	3.61E-60	-1.69E-46
MS.gene055466	MS.gene070241	0.861222	1.19E-63	-1.69E-46
MS.gene055468	MS.gene070241	0.856998	2.20E-62	-1.69E-46
MS.gene055469	MS.gene070241	0.828643	8.02E-55	-1.69E-46
MS.gene055513	MS.gene070241	0.815886	7.35E-52	-1.69E-46
MS.gene055580	MS.gene070241	-0.807413	5.12E-50	-1.69E-46
MS.gene055778	MS.gene070241	0.816163	6.37E-52	-1.69E-46
MS.gene055818	MS.gene070241	0.807247	5.55E-50	-1.69E-46
MS.gene056043	MS.gene070241	-0.802453	5.57E-49	-1.69E-46
MS.gene05608	MS.gene070241	0.802598	5.20E-49	-1.69E-46
MS.gene056215	MS.gene070241	0.84998	2.28E-60	-1.69E-46
MS.gene056308	MS.gene070241	0.841093	5.85E-58	-1.69E-46
MS.gene056580	MS.gene070241	0.816896	4.37E-52	-1.69E-46
MS.gene057446	MS.gene070241	0.812031	5.20E-51	-1.69E-46
MS.gene057693	MS.gene070241	0.815389	9.48E-52	-1.69E-46
MS.gene057705	MS.gene070241	0.810116	1.35E-50	-1.69E-46
MS.gene057869	MS.gene070241	0.800744	1.25E-48	-1.69E-46
MS.gene057915	MS.gene070241	0.810286	1.24E-50	-1.69E-46
MS.gene058084	MS.gene070241	0.802705	4.94E-49	-1.69E-46
MS.gene058636	MS.gene070241	0.811166	8.02E-51	-1.69E-46
MS.gene058852	MS.gene070241	0.81077	9.77E-51	-1.69E-46
MS.gene058906	MS.gene070241	0.817368	3.42E-52	-1.69E-46
MS.gene059725	MS.gene070241	0.816587	5.12E-52	-1.69E-46
MS.gene05978	MS.gene070241	0.81242	4.28E-51	-1.69E-46
MS.gene059842	MS.gene070241	0.808112	3.63E-50	-1.69E-46
MS.gene059937	MS.gene070241	0.801389	9.22E-49	-1.69E-46
MS.gene06001	MS.gene070241	0.807992	3.85E-50	-1.69E-46
MS.gene060429	MS.gene070241	0.846018	2.83E-59	-1.69E-46
MS.gene060587	MS.gene070241	0.826553	2.55E-54	-1.69E-46
MS.gene060803	MS.gene070241	0.835839	1.33E-56	-1.69E-46
MS.gene061006	MS.gene070241	0.810879	9.26E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene070241	MS.gene048705	PPI
MS.gene070241	MS.gene061876	PPI
MS.gene070241	MS.gene061874	PPI
MS.gene070241	MS.gene04576	PPI
MS.gene070241	MS.gene056215	PPI
MS.gene070241	MS.gene061869	PPI
MS.gene070241	MS.gene64920	PPI
MS.gene070241	MS.gene04452	PPI
MS.gene070241	MS.gene070244	PPI
MS.gene78578	MS.gene070241	PPI
MS.gene070241	MS.gene038469	PPI
MS.gene070241	MS.gene061867	PPI
MS.gene070241	MS.gene008276	PPI
MS.gene070241	MS.gene30920	PPI
MS.gene070241	MS.gene012391	PPI
MS.gene070241	MS.gene05619	PPI
MS.gene070241	MS.gene73136	PPI
MS.gene070241	MS.gene049821	PPI
MS.gene070241	MS.gene78578	PPI
MS.gene070241	MS.gene04068	PPI
MS.gene070241	MS.gene022946	PPI
MS.gene070241	MS.gene035719	PPI
MS.gene070241	MS.gene05623	PPI
MS.gene070241	MS.gene061872	PPI
MS.gene070241	MS.gene89488	PPI
MS.gene04067	MS.gene070241	PPI
MS.gene71215	MS.gene070241	PPI
MS.gene070241	MS.gene66225	PPI
MS.gene070241	MS.gene035720	PPI
MS.gene070241	MS.gene70955	PPI
MS.gene070241	MS.gene61017	PPI
MS.gene070241	MS.gene09871	PPI
MS.gene070241	MS.gene012390	PPI
MS.gene070241	MS.gene08121	PPI
MS.gene070241	MS.gene04067	PPI
MS.gene070241	MS.gene064557	PPI
MS.gene071136	MS.gene070241	PPI
MS.gene070241	MS.gene035787	PPI
MS.gene070241	MS.gene04434	PPI
MS.gene070241	MS.gene070907	PPI
MS.gene070241	MS.gene04392	PPI
MS.gene070241	MS.gene060463	PPI
MS.gene070241	MS.gene89487	PPI
MS.gene070241	MS.gene010219	PPI
MS.gene070241	MS.gene04433	PPI
MS.gene070241	MS.gene040383	PPI
MS.gene070241	MS.gene68545	PPI
MS.gene070241	MS.gene070242	PPI
MS.gene070241	MS.gene016624	PPI
MS.gene056215	MS.gene070241	PPI
MS.gene070241	MS.gene048746	PPI
MS.gene070241	MS.gene71215	PPI
MS.gene035787	MS.gene070241	PPI
MS.gene070241	MS.gene028629	PPI
MS.gene070241	MS.gene58854	PPI
MS.gene070241	MS.gene043967	PPI
MS.gene070241	MS.gene31196	PPI
MS.gene070241	MS.gene060465	PPI
MS.gene070241	MS.gene035897	PPI
MS.gene070241	MS.gene049705	PPI
MS.gene070241	MS.gene061870	PPI
MS.gene070241	MS.gene05608	PPI
MS.gene070241	MS.gene035896	PPI
MS.gene070241	MS.gene88681	PPI
MS.gene070241	MS.gene82546	PPI
MS.gene061876	MS.gene070241	PPI
MS.gene070241	MS.gene60093	PPI
MS.gene070241	MS.gene08122	PPI
MS.gene012391	MS.gene070241	PPI
MS.gene070241	MS.gene09873	PPI
MS.gene070241	MS.gene035718	PPI
MS.gene070241	MS.gene022932	PPI
MS.gene060463	MS.gene070241	PPI
MS.gene070241	MS.gene010220	PPI
MS.gene070241	MS.gene04972	PPI
MS.gene070241	MS.gene004121	PPI
MS.gene070241	MS.gene62520	PPI
MS.gene070241	MS.gene08053	PPI
MS.gene070241	MS.gene018578	PPI
MS.gene070241	MS.gene035721	PPI
MS.gene070241	MS.gene009027	PPI
MS.gene070241	MS.gene04064	PPI
MS.gene070241	MS.gene018580	PPI
MS.gene070241	MS.gene004124	PPI
MS.gene070241	MS.gene071136	PPI
MS.gene070241	MS.gene88680	PPI
MS.gene070241	MS.gene072493	PPI
MS.gene070241	MS.gene04052	PPI
MS.gene070241	MS.gene84536	PPI
MS.gene070241	MS.gene061866	PPI
MS.gene070241	MS.gene64650	PPI
MS.gene070241	MS.gene79195	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070241.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070241.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070241.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070241.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070241.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070241.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070241.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070241.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070241.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070241.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070241.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070241.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070241.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070241.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene070241.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene070241.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070241.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene070241.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene070241.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene070241.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene070241.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene070241.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene070241.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene070241.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene070241.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene070241.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene070241.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 30 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTTCTCCGCGACAACATTC+AGG	0.329692	8.2:+6162316	MS.gene070241:CDS
GGAAGGACTCTTTACGGTTT+CGG	0.343515	8.2:+6162536	MS.gene070241:CDS
GGCAAAGGAGGAAAGGGTTT+AGG	0.384869	8.2:+6162266	MS.gene070241:CDS
AGGCAGGGAAGGACTCTTTA+CGG	0.424564	8.2:+6162530	MS.gene070241:CDS
GGAGGAAAGGGTTTAGGAAA+GGG	0.429069	8.2:+6162272	MS.gene070241:CDS
TCAACTTTCGCAGCTATGTC+AGG	0.463992	8.2:+6162239	None:intergenic
TGTTTACGCTCTCAAGAGGC+AGG	0.493747	8.2:+6162514	MS.gene070241:CDS
TGTTGTCGCGGAGAACCTTA+CGG	0.502023	8.2:-6162310	None:intergenic
AGGAAGAGGCAAAGGAGGAA+AGG	0.518412	8.2:+6162259	MS.gene070241:CDS
GGAAGAGGCAAAGGAGGAAA+GGG	0.527098	8.2:+6162260	MS.gene070241:CDS
AGGAGGAAAGGGTTTAGGAA+AGG	0.528235	8.2:+6162271	MS.gene070241:CDS
GGAAAGGGTTTAGGAAAGGG+AGG	0.536831	8.2:+6162275	MS.gene070241:CDS
CCATCGCAGTAACAGTCTTA+CGG	0.545551	8.2:-6162487	None:intergenic
GTTCTCCGCGACAACATTCA+GGG	0.554706	8.2:+6162317	MS.gene070241:CDS
CCGTAAGACTGTTACTGCGA+TGG	0.561095	8.2:+6162487	MS.gene070241:CDS
TTCGCAGCTATGTCAGGAAG+AGG	0.562303	8.2:+6162245	None:intergenic
GCTATGTCAGGAAGAGGCAA+AGG	0.571124	8.2:+6162251	None:intergenic
AGGAGCAAAACGACACCGTA+AGG	0.575994	8.2:+6162295	MS.gene070241:CDS
ACGCTCTCAAGAGGCAGGGA+AGG	0.583359	8.2:+6162519	MS.gene070241:CDS
CTTGCAAGACGACGAATCGC+AGG	0.588436	8.2:-6162350	None:intergenic
ACGGCGAGCATGCTCTGTGT+AGG	0.590446	8.2:-6162468	None:intergenic
ATGTCAGGAAGAGGCAAAGG+AGG	0.592343	8.2:+6162254	MS.gene070241:CDS
ATGTTGTTTACGCTCTCAAG+AGG	0.616421	8.2:+6162510	MS.gene070241:CDS
GTTTACGCTCTCAAGAGGCA+GGG	0.624823	8.2:+6162515	MS.gene070241:CDS
CGTCGTCTTGCAAGACGTGG+TGG	0.633046	8.2:+6162359	MS.gene070241:CDS
TTAATCTATGAAGAAACTCG+TGG	0.634992	8.2:+6162401	MS.gene070241:CDS
TAATTCCCTGAATGTTGTCG+CGG	0.640736	8.2:-6162322	None:intergenic
ATTCGTCGTCTTGCAAGACG+TGG	0.646636	8.2:+6162356	MS.gene070241:CDS
AGGACTCTTTACGGTTTCGG+CGG	0.659680	8.2:+6162539	MS.gene070241:CDS
GGTGGTGTGAAACGTATCAG+TGG	0.722771	8.2:+6162377	MS.gene070241:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTAATCTATGAAGAAACTCG+TGG	+	chr8.2:6162401-6162420	MS.gene070241:CDS	30.0%
	TAATTCCCTGAATGTTGTCG+CGG	-	chr8.2:6162325-6162344	None:intergenic	40.0%
!	ATGTTGTTTACGCTCTCAAG+AGG	+	chr8.2:6162510-6162529	MS.gene070241:CDS	40.0%
	AGGAGGAAAGGGTTTAGGAA+AGG	+	chr8.2:6162271-6162290	MS.gene070241:CDS	45.0%
	CCATCGCAGTAACAGTCTTA+CGG	-	chr8.2:6162490-6162509	None:intergenic	45.0%
	GGAAGGACTCTTTACGGTTT+CGG	+	chr8.2:6162536-6162555	MS.gene070241:CDS	45.0%
	GGAGGAAAGGGTTTAGGAAA+GGG	+	chr8.2:6162272-6162291	MS.gene070241:CDS	45.0%
	AGGAAGAGGCAAAGGAGGAA+AGG	+	chr8.2:6162259-6162278	MS.gene070241:CDS	50.0%
	AGGACTCTTTACGGTTTCGG+CGG	+	chr8.2:6162539-6162558	MS.gene070241:CDS	50.0%
	AGGAGCAAAACGACACCGTA+AGG	+	chr8.2:6162295-6162314	MS.gene070241:CDS	50.0%
	AGGCAGGGAAGGACTCTTTA+CGG	+	chr8.2:6162530-6162549	MS.gene070241:CDS	50.0%
	ATGTCAGGAAGAGGCAAAGG+AGG	+	chr8.2:6162254-6162273	MS.gene070241:CDS	50.0%
	ATTCGTCGTCTTGCAAGACG+TGG	+	chr8.2:6162356-6162375	MS.gene070241:CDS	50.0%
	CCGTAAGACTGTTACTGCGA+TGG	+	chr8.2:6162487-6162506	MS.gene070241:CDS	50.0%
	GGAAAGGGTTTAGGAAAGGG+AGG	+	chr8.2:6162275-6162294	MS.gene070241:CDS	50.0%
	GGAAGAGGCAAAGGAGGAAA+GGG	+	chr8.2:6162260-6162279	MS.gene070241:CDS	50.0%
	GGCAAAGGAGGAAAGGGTTT+AGG	+	chr8.2:6162266-6162285	MS.gene070241:CDS	50.0%
	GGTGGTGTGAAACGTATCAG+TGG	+	chr8.2:6162377-6162396	MS.gene070241:CDS	50.0%
	GTTCTCCGCGACAACATTCA+GGG	+	chr8.2:6162317-6162336	MS.gene070241:CDS	50.0%
	GTTTACGCTCTCAAGAGGCA+GGG	+	chr8.2:6162515-6162534	MS.gene070241:CDS	50.0%
	TGTTGTCGCGGAGAACCTTA+CGG	-	chr8.2:6162313-6162332	None:intergenic	50.0%
	TGTTTACGCTCTCAAGAGGC+AGG	+	chr8.2:6162514-6162533	MS.gene070241:CDS	50.0%
	CTTGCAAGACGACGAATCGC+AGG	-	chr8.2:6162353-6162372	None:intergenic	55.0%
	GGTTCTCCGCGACAACATTC+AGG	+	chr8.2:6162316-6162335	MS.gene070241:CDS	55.0%
	ACGCTCTCAAGAGGCAGGGA+AGG	+	chr8.2:6162519-6162538	MS.gene070241:CDS	60.0%
	ACGGCGAGCATGCTCTGTGT+AGG	-	chr8.2:6162471-6162490	None:intergenic	60.0%
	CGTCGTCTTGCAAGACGTGG+TGG	+	chr8.2:6162359-6162378	MS.gene070241:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	6162254	6162565	6162254	ID=MS.gene070241
chr8.2	mRNA	6162254	6162565	6162254	ID=MS.gene070241.t1;Parent=MS.gene070241
chr8.2	exon	6162254	6162565	6162254	ID=MS.gene070241.t1.exon1;Parent=MS.gene070241.t1
chr8.2	CDS	6162254	6162565	6162254	ID=cds.MS.gene070241.t1;Parent=MS.gene070241.t1

Gene Sequence	>MS.gene070241 ATGTCAGGAAGAGGCAAAGGAGGAAAGGGTTTAGGAAAGGGAGGAGCAAAACGACACCGTAAGGTTCTCCGCGACAACATTCAGGGAATTACAAAGCCTGCGATTCGTCGTCTTGCAAGACGTGGTGGTGTGAAACGTATCAGTGGCTTAATCTATGAAGAAACTCGTGGTGTTCTCAAAATCTTTCTTGAGAATGTGATTCGTGATGCTGTCACCTACACAGAGCATGCTCGCCGTAAGACTGTTACTGCGATGGATGTTGTTTACGCTCTCAAGAGGCAGGGAAGGACTCTTTACGGTTTCGGCGGTTAG
Protein sequence	>MS.gene070241.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG