AlfalfaGEDB | MS.gene028629

M. sativa cultivar XinJiangDaYe / MS.gene028629

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028629.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028629.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028629.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene028629	MS.gene58854	PPI
MS.gene028629	MS.gene035720	PPI
MS.gene028629	MS.gene043967	PPI
MS.gene028629	MS.gene31196	PPI
MS.gene71215	MS.gene028629	PPI
MS.gene028629	MS.gene060465	PPI
MS.gene061870	MS.gene028629	PPI
MS.gene028629	MS.gene035897	PPI
MS.gene028629	MS.gene049705	PPI
MS.gene028629	MS.gene061870	PPI
MS.gene028629	MS.gene070907	PPI
MS.gene035718	MS.gene028629	PPI
MS.gene028629	MS.gene05608	PPI
MS.gene071136	MS.gene028629	PPI
MS.gene028629	MS.gene070242	PPI
MS.gene028629	MS.gene88681	PPI
MS.gene028629	MS.gene82546	PPI
MS.gene78578	MS.gene028629	PPI
MS.gene028629	MS.gene60093	PPI
MS.gene028629	MS.gene08122	PPI
MS.gene028629	MS.gene09873	PPI
MS.gene028629	MS.gene035718	PPI
MS.gene028629	MS.gene022932	PPI
MS.gene028629	MS.gene010220	PPI
MS.gene028629	MS.gene04972	PPI
MS.gene035787	MS.gene028629	PPI
MS.gene04067	MS.gene028629	PPI
MS.gene028629	MS.gene62520	PPI
MS.gene028629	MS.gene08053	PPI
MS.gene028629	MS.gene018578	PPI
MS.gene028629	MS.gene035721	PPI
MS.gene028629	MS.gene018580	PPI
MS.gene028629	MS.gene004124	PPI
MS.gene028629	MS.gene88680	PPI
MS.gene30920	MS.gene028629	PPI
MS.gene028629	MS.gene072493	PPI
MS.gene061872	MS.gene028629	PPI
MS.gene028629	MS.gene04052	PPI
MS.gene028629	MS.gene84536	PPI
MS.gene028629	MS.gene035896	PPI
MS.gene028629	MS.gene061866	PPI
MS.gene88680	MS.gene028629	PPI
MS.gene028629	MS.gene64650	PPI
MS.gene070241	MS.gene028629	PPI
MS.gene028629	MS.gene79195	PPI
MS.gene028629	MS.gene048705	PPI
MS.gene056215	MS.gene028629	PPI
MS.gene028629	MS.gene061874	PPI
MS.gene028629	MS.gene061869	PPI
MS.gene061876	MS.gene028629	PPI
MS.gene012391	MS.gene028629	PPI
MS.gene028629	MS.gene04452	PPI
MS.gene028629	MS.gene070244	PPI
MS.gene028629	MS.gene004121	PPI
MS.gene060463	MS.gene028629	PPI
MS.gene028629	MS.gene009027	PPI
MS.gene028629	MS.gene038469	PPI
MS.gene028629	MS.gene061867	PPI
MS.gene028629	MS.gene04064	PPI
MS.gene028629	MS.gene008276	PPI
MS.gene028629	MS.gene30920	PPI
MS.gene028629	MS.gene05619	PPI
MS.gene028629	MS.gene73136	PPI
MS.gene028629	MS.gene049821	PPI
MS.gene028629	MS.gene04068	PPI
MS.gene028629	MS.gene035719	PPI
MS.gene028629	MS.gene05623	PPI
MS.gene028629	MS.gene061872	PPI
MS.gene028629	MS.gene89488	PPI
MS.gene028629	MS.gene66225	PPI
MS.gene028629	MS.gene70955	PPI
MS.gene028629	MS.gene61017	PPI
MS.gene028629	MS.gene04576	PPI
MS.gene028629	MS.gene09871	PPI
MS.gene028629	MS.gene012390	PPI
MS.gene028629	MS.gene08121	PPI
MS.gene028629	MS.gene64920	PPI
MS.gene028629	MS.gene064557	PPI
MS.gene028629	MS.gene04434	PPI
MS.gene028629	MS.gene04392	PPI
MS.gene028629	MS.gene89487	PPI
MS.gene070244	MS.gene028629	PPI
MS.gene028629	MS.gene010219	PPI
MS.gene028629	MS.gene04433	PPI
MS.gene028629	MS.gene040383	PPI
MS.gene028629	MS.gene68545	PPI
MS.gene009027	MS.gene028629	PPI
MS.gene028629	MS.gene016624	PPI
MS.gene31196	MS.gene028629	PPI
MS.gene060465	MS.gene028629	PPI
MS.gene028629	MS.gene048746	PPI
MS.gene028629	MS.gene022946	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028629.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene028629.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene028629.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene028629.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene028629.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene028629.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene028629.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene028629.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene028629.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene028629.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene028629.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene028629.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene028629.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene028629.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene028629.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene028629.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028629.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene028629.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene028629.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene028629.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene028629.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene028629.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene028629.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene028629.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene028629.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene028629.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene028629.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 32 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGACAAGGAAGGACTCTTTA+TGG	0.217305	2.3:-6408826	MS.gene028629:CDS
GGAAGGACTCTTTATGGATT+TGG	0.266178	2.3:-6408820	MS.gene028629:CDS
GAAGGACTCTTTATGGATTT+GGG	0.322097	2.3:-6408819	MS.gene028629:CDS
TGGAAAAGGTGGAAAGGGTT+TGG	0.322287	2.3:-6409091	MS.gene028629:CDS
TTGTTGCGAATCAAGATGTC+TGG	0.363585	2.3:-6409117	None:intergenic
GGAAAAGGTGGAAAGGGTTT+GGG	0.363863	2.3:-6409090	MS.gene028629:CDS
GGAAAGGGTTTGGGAAAGGG+AGG	0.393661	2.3:-6409081	MS.gene028629:CDS
GAAACCCGCTATTCGTCGTT+TGG	0.435441	2.3:-6409010	MS.gene028629:CDS
GGTGGAAAGGGTTTGGGAAA+GGG	0.444952	2.3:-6409084	MS.gene028629:CDS
AAGATGTCTGGGCGTGGAAA+AGG	0.454402	2.3:-6409105	MS.gene028629:CDS
AGGTGGAAAGGGTTTGGGAA+AGG	0.475381	2.3:-6409085	MS.gene028629:CDS
GTGCTTCGTGATAACATTCA+AGG	0.499787	2.3:-6409039	MS.gene028629:CDS
GAGAAAGACTGTTACTGCTA+TGG	0.515154	2.3:-6408869	MS.gene028629:CDS
CGTCGTTTGGCACGAAGAGG+AGG	0.535271	2.3:-6408997	MS.gene028629:CDS
TGTTGCGAATCAAGATGTCT+GGG	0.537404	2.3:-6409116	None:intergenic
CGTGCCAAACGACGAATAGC+GGG	0.539788	2.3:+6409006	None:intergenic
TGTTATCACGAAGCACCTTA+CGG	0.542642	2.3:+6409046	None:intergenic
CGAATCAAGATGTCTGGGCG+TGG	0.544605	2.3:-6409111	None:intergenic
TTACTTACACAGAGCACGCT+AGG	0.553235	2.3:-6408891	MS.gene028629:CDS
ATGTCTGGGCGTGGAAAAGG+TGG	0.568080	2.3:-6409102	MS.gene028629:CDS
AGGAGCAAAACGACACCGTA+AGG	0.575994	2.3:-6409061	MS.gene028629:CDS
GGAGAATGTTATTCGTGATG+CGG	0.582811	2.3:-6408914	MS.gene028629:CDS
GGGCGTGGAAAAGGTGGAAA+GGG	0.585493	2.3:-6409096	MS.gene028629:CDS
AAGGACTCTTTATGGATTTG+GGG	0.603730	2.3:-6408818	MS.gene028629:CDS
TCGTGCCAAACGACGAATAG+CGG	0.606775	2.3:+6409005	None:intergenic
GGAGGAGTGAAGCGTATCAG+CGG	0.620100	2.3:-6408979	MS.gene028629:CDS
ATTCGTCGTTTGGCACGAAG+AGG	0.624143	2.3:-6409000	MS.gene028629:CDS
TGGGCGTGGAAAAGGTGGAA+AGG	0.624585	2.3:-6409097	MS.gene028629:CDS
GTTTATGCATTGAAGAGACA+AGG	0.629886	2.3:-6408841	MS.gene028629:CDS
ATGCATTGAAGAGACAAGGA+AGG	0.634793	2.3:-6408837	MS.gene028629:CDS
AGGACTCTTTATGGATTTGG+GGG	0.645439	2.3:-6408817	MS.gene028629:CDS
TTGATCTACGAAGAGACACG+TGG	0.703849	2.3:-6408955	MS.gene028629:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGGACTCTTTATGGATTTG+GGG	-	chr2.3:6409097-6409116	MS.gene028629:CDS	35.0%
	GAAGGACTCTTTATGGATTT+GGG	-	chr2.3:6409096-6409115	MS.gene028629:CDS	35.0%
	GTTTATGCATTGAAGAGACA+AGG	-	chr2.3:6409074-6409093	MS.gene028629:CDS	35.0%
	AGACAAGGAAGGACTCTTTA+TGG	-	chr2.3:6409089-6409108	MS.gene028629:CDS	40.0%
	AGGACTCTTTATGGATTTGG+GGG	-	chr2.3:6409098-6409117	MS.gene028629:CDS	40.0%
	ATGCATTGAAGAGACAAGGA+AGG	-	chr2.3:6409078-6409097	MS.gene028629:CDS	40.0%
	GAGAAAGACTGTTACTGCTA+TGG	-	chr2.3:6409046-6409065	MS.gene028629:CDS	40.0%
	GGAAGGACTCTTTATGGATT+TGG	-	chr2.3:6409095-6409114	MS.gene028629:CDS	40.0%
	GGAGAATGTTATTCGTGATG+CGG	-	chr2.3:6409001-6409020	MS.gene028629:CDS	40.0%
	GTGCTTCGTGATAACATTCA+AGG	-	chr2.3:6408876-6408895	MS.gene028629:CDS	40.0%
!	TGGCGTGTTGAAGATCTTTT+TGG	-	chr2.3:6408980-6408999	MS.gene028629:CDS	40.0%
!	TGTTATCACGAAGCACCTTA+CGG	+	chr2.3:6408872-6408891	None:intergenic	40.0%
	GGAAAAGGTGGAAAGGGTTT+GGG	-	chr2.3:6408825-6408844	MS.gene028629:CDS	45.0%
	TGGAAAAGGTGGAAAGGGTT+TGG	-	chr2.3:6408824-6408843	MS.gene028629:CDS	45.0%
	TTACTTACACAGAGCACGCT+AGG	-	chr2.3:6409024-6409043	MS.gene028629:CDS	45.0%
	TTGATCTACGAAGAGACACG+TGG	-	chr2.3:6408960-6408979	MS.gene028629:CDS	45.0%
	AGGAGCAAAACGACACCGTA+AGG	-	chr2.3:6408854-6408873	MS.gene028629:CDS	50.0%
	AGGTGGAAAGGGTTTGGGAA+AGG	-	chr2.3:6408830-6408849	MS.gene028629:CDS	50.0%
	ATTCGTCGTTTGGCACGAAG+AGG	-	chr2.3:6408915-6408934	MS.gene028629:CDS	50.0%
	GAAACCCGCTATTCGTCGTT+TGG	-	chr2.3:6408905-6408924	MS.gene028629:CDS	50.0%
	GGTGGAAAGGGTTTGGGAAA+GGG	-	chr2.3:6408831-6408850	MS.gene028629:CDS	50.0%
	TCGTGCCAAACGACGAATAG+CGG	+	chr2.3:6408913-6408932	None:intergenic	50.0%
	ATGTCTGGGCGTGGAAAAGG+TGG	-	chr2.3:6408813-6408832	MS.gene028629:CDS	55.0%
	CGTGCCAAACGACGAATAGC+GGG	+	chr2.3:6408912-6408931	None:intergenic	55.0%
	GGAAAGGGTTTGGGAAAGGG+AGG	-	chr2.3:6408834-6408853	MS.gene028629:CDS	55.0%
	GGAGGAGTGAAGCGTATCAG+CGG	-	chr2.3:6408936-6408955	MS.gene028629:CDS	55.0%
	GGGCGTGGAAAAGGTGGAAA+GGG	-	chr2.3:6408819-6408838	MS.gene028629:CDS	55.0%
	TGGGCGTGGAAAAGGTGGAA+AGG	-	chr2.3:6408818-6408837	MS.gene028629:CDS	55.0%
	CGTCGTTTGGCACGAAGAGG+AGG	-	chr2.3:6408918-6408937	MS.gene028629:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	6408813	6409124	6408813	ID=MS.gene028629
chr2.3	mRNA	6408813	6409124	6408813	ID=MS.gene028629.t1;Parent=MS.gene028629
chr2.3	exon	6408813	6409124	6408813	ID=MS.gene028629.t1.exon1;Parent=MS.gene028629.t1
chr2.3	CDS	6408813	6409124	6408813	ID=cds.MS.gene028629.t1;Parent=MS.gene028629.t1

Gene Sequence	>MS.gene028629 ATGTCTGGGCGTGGAAAAGGTGGAAAGGGTTTGGGAAAGGGAGGAGCAAAACGACACCGTAAGGTGCTTCGTGATAACATTCAAGGAATAACGAAACCCGCTATTCGTCGTTTGGCACGAAGAGGAGGAGTGAAGCGTATCAGCGGTTTGATCTACGAAGAGACACGTGGCGTGTTGAAGATCTTTTTGGAGAATGTTATTCGTGATGCGGTTACTTACACAGAGCACGCTAGGAGAAAGACTGTTACTGCTATGGATGTTGTTTATGCATTGAAGAGACAAGGAAGGACTCTTTATGGATTTGGGGGTTAG
Protein sequence	>MS.gene028629.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG