AlfalfaGEDB | MS.gene010219

M. sativa cultivar XinJiangDaYe / MS.gene010219

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010219.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010219.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010219.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene010219	MS.gene62520	PPI
MS.gene66225	MS.gene010219	PPI
MS.gene071136	MS.gene010219	PPI
MS.gene070242	MS.gene010219	PPI
MS.gene012390	MS.gene010219	PPI
MS.gene010219	MS.gene004124	PPI
MS.gene79195	MS.gene010219	PPI
MS.gene035721	MS.gene010219	PPI
MS.gene056215	MS.gene010219	PPI
MS.gene064557	MS.gene010219	PPI
MS.gene038469	MS.gene010219	PPI
MS.gene010219	MS.gene04052	PPI
MS.gene010219	MS.gene84536	PPI
MS.gene010219	MS.gene061866	PPI
MS.gene82546	MS.gene010219	PPI
MS.gene61017	MS.gene010219	PPI
MS.gene04433	MS.gene010219	PPI
MS.gene04452	MS.gene010219	PPI
MS.gene010219	MS.gene64650	PPI
MS.gene010219	MS.gene60093	PPI
MS.gene010219	MS.gene061874	PPI
MS.gene035787	MS.gene010219	PPI
MS.gene010219	MS.gene061869	PPI
MS.gene89487	MS.gene010219	PPI
MS.gene035719	MS.gene010219	PPI
MS.gene010219	MS.gene04972	PPI
MS.gene58854	MS.gene010219	PPI
MS.gene010219	MS.gene004121	PPI
MS.gene70955	MS.gene010219	PPI
MS.gene040383	MS.gene010219	PPI
MS.gene010219	MS.gene018578	PPI
MS.gene04392	MS.gene010219	PPI
MS.gene072493	MS.gene010219	PPI
MS.gene035720	MS.gene010219	PPI
MS.gene30920	MS.gene010219	PPI
MS.gene048746	MS.gene010219	PPI
MS.gene010220	MS.gene010219	PPI
MS.gene08053	MS.gene010219	PPI
MS.gene061872	MS.gene010219	PPI
MS.gene010219	MS.gene061867	PPI
MS.gene010219	MS.gene018580	PPI
MS.gene010219	MS.gene008276	PPI
MS.gene88680	MS.gene010219	PPI
MS.gene010219	MS.gene05619	PPI
MS.gene070241	MS.gene010219	PPI
MS.gene049821	MS.gene010219	PPI
MS.gene022946	MS.gene010219	PPI
MS.gene62520	MS.gene010219	PPI
MS.gene04064	MS.gene010219	PPI
MS.gene010219	MS.gene89488	PPI
MS.gene061876	MS.gene010219	PPI
MS.gene05608	MS.gene010219	PPI
MS.gene010219	MS.gene048705	PPI
MS.gene010219	MS.gene61017	PPI
MS.gene012391	MS.gene010219	PPI
MS.gene010219	MS.gene04576	PPI
MS.gene070244	MS.gene010219	PPI
MS.gene09871	MS.gene010219	PPI
MS.gene035897	MS.gene010219	PPI
MS.gene64920	MS.gene010219	PPI
MS.gene010219	MS.gene012390	PPI
MS.gene04576	MS.gene010219	PPI
MS.gene060463	MS.gene010219	PPI
MS.gene31196	MS.gene010219	PPI
MS.gene010219	MS.gene016624	PPI
MS.gene04068	MS.gene010219	PPI
MS.gene010219	MS.gene73136	PPI
MS.gene04434	MS.gene010219	PPI
MS.gene08121	MS.gene010219	PPI
MS.gene022932	MS.gene010219	PPI
MS.gene010219	MS.gene035720	PPI
MS.gene88681	MS.gene010219	PPI
MS.gene035718	MS.gene010219	PPI
MS.gene035896	MS.gene010219	PPI
MS.gene73136	MS.gene010219	PPI
MS.gene008276	MS.gene010219	PPI
MS.gene070907	MS.gene010219	PPI
MS.gene010219	MS.gene049705	PPI
MS.gene009027	MS.gene010219	PPI
MS.gene09873	MS.gene010219	PPI
MS.gene78578	MS.gene010219	PPI
MS.gene04052	MS.gene010219	PPI
MS.gene010219	MS.gene05608	PPI
MS.gene08122	MS.gene010219	PPI
MS.gene060465	MS.gene010219	PPI
MS.gene010219	MS.gene68545	PPI
MS.gene048705	MS.gene010219	PPI
MS.gene05623	MS.gene010219	PPI
MS.gene043967	MS.gene010219	PPI
MS.gene84536	MS.gene010219	PPI
MS.gene028629	MS.gene010219	PPI
MS.gene04067	MS.gene010219	PPI
MS.gene71215	MS.gene010219	PPI
MS.gene010219	MS.gene09873	PPI
MS.gene061866	MS.gene010219	PPI
MS.gene061870	MS.gene010219	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010219.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010219.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010219.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010219.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010219.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010219.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010219.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010219.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010219.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010219.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010219.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010219.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010219.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010219.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene010219.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene010219.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010219.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene010219.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene010219.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene010219.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene010219.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene010219.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene010219.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene010219.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene010219.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene010219.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene010219.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 35 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGCAAGGAAGGACCCTTTA+TGG	0.289623	5.1:-48826770	MS.gene010219:CDS
GGAAGGACCCTTTATGGTTT+CGG	0.347466	5.1:-48826764	MS.gene010219:CDS
CCTAACCACCGAAACCATAA+AGG	0.360537	5.1:+48826756	None:intergenic
CCTTTATGGTTTCGGTGGTT+AGG	0.360991	5.1:-48826756	None:intergenic
GGCAAAGGAGGAAAGGGTTT+AGG	0.384869	5.1:-48827034	MS.gene010219:CDS
GTTCTTCGAGATAACATTCA+AGG	0.432889	5.1:-48826983	MS.gene010219:CDS
GAGACGACGGATCGCCGGTT+TGG	0.447802	5.1:+48826955	None:intergenic
ATCTTGCAATCAACAATGTC+AGG	0.473641	5.1:-48827061	None:intergenic
TGTTATCTCGAAGAACTTTG+CGG	0.480041	5.1:+48826990	None:intergenic
TCGCAAGACGGTGACTGCTA+TGG	0.501230	5.1:-48826813	MS.gene010219:CDS
CTAACCACCGAAACCATAAA+GGG	0.505014	5.1:+48826757	None:intergenic
GGAGGAAAGGGTTTAGGAAA+GGG	0.517349	5.1:-48827028	MS.gene010219:CDS
AGGAAGAGGCAAAGGAGGAA+AGG	0.518412	5.1:-48827041	MS.gene010219:CDS
GCGACGAGCGTGTTCAGTGT+AGG	0.523201	5.1:+48826832	None:intergenic
GGAAGAGGCAAAGGAGGAAA+GGG	0.527098	5.1:-48827040	MS.gene010219:CDS
CATTCAAGGTATCACCAAAC+CGG	0.533301	5.1:-48826969	MS.gene010219:CDS
CGGCGATCCGTCGTCTCGCA+AGG	0.536114	5.1:-48826949	MS.gene010219:CDS
AAATATCTTGAGAACTCCGC+GGG	0.550551	5.1:+48826883	None:intergenic
ATCCGTCGTCTCGCAAGGCG+TGG	0.568424	5.1:-48826944	MS.gene010219:CDS
CTTGCGAGACGACGGATCGC+CGG	0.577326	5.1:+48826950	None:intergenic
GTTTATGCGCTCAAGAGGCA+AGG	0.577445	5.1:-48826785	MS.gene010219:CDS
CAATCAACAATGTCAGGAAG+AGG	0.577531	5.1:-48827055	None:intergenic
ATGCGCTCAAGAGGCAAGGA+AGG	0.588067	5.1:-48826781	MS.gene010219:CDS
AGGACCCTTTATGGTTTCGG+TGG	0.597859	5.1:-48826761	MS.gene010219:CDS
ACAATGTCAGGAAGAGGCAA+AGG	0.598135	5.1:-48827049	MS.gene010219:CDS
ATGTCAGGAAGAGGCAAAGG+AGG	0.604597	5.1:-48827046	MS.gene010219:CDS
ATGTTGTTTATGCGCTCAAG+AGG	0.611083	5.1:-48826790	MS.gene010219:CDS
CACCACGCCTTGCGAGACGA+CGG	0.613576	5.1:+48826942	None:intergenic
AGGAGGAAAGGGTTTAGGAA+AGG	0.616515	5.1:-48827029	MS.gene010219:CDS
CGTCGTCTCGCAAGGCGTGG+TGG	0.632134	5.1:-48826941	MS.gene010219:CDS
GGTGGTGTTAAGCGTATCAG+TGG	0.637620	5.1:-48826923	MS.gene010219:CDS
TGAACACGCTCGTCGCAAGA+CGG	0.661743	5.1:-48826825	MS.gene010219:CDS
GGAAAGGGTTTAGGAAAGGG+CGG	0.700891	5.1:-48827025	MS.gene010219:CDS
CTCATCTATGAAGAAACCCG+CGG	0.706099	5.1:-48826899	MS.gene010219:CDS
GAAATATCTTGAGAACTCCG+CGG	0.726776	5.1:+48826882	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GTTCTTCGAGATAACATTCA+AGG	-	chr5.1:48826820-48826839	MS.gene010219:CDS	35.0%
	TGTTATCTCGAAGAACTTTG+CGG	+	chr5.1:48826816-48826835	None:intergenic	35.0%
	AAATATCTTGAGAACTCCGC+GGG	+	chr5.1:48826923-48826942	None:intergenic	40.0%
	CTAACCACCGAAACCATAAA+GGG	+	chr5.1:48827049-48827068	None:intergenic	40.0%
	GAAATATCTTGAGAACTCCG+CGG	+	chr5.1:48826924-48826943	None:intergenic	40.0%
!	ATGTTGTTTATGCGCTCAAG+AGG	-	chr5.1:48827013-48827032	MS.gene010219:CDS	40.0%
!	CATTCAAGGTATCACCAAAC+CGG	-	chr5.1:48826834-48826853	MS.gene010219:CDS	40.0%
	AGGAGGAAAGGGTTTAGGAA+AGG	-	chr5.1:48826774-48826793	MS.gene010219:CDS	45.0%
	CTCATCTATGAAGAAACCCG+CGG	-	chr5.1:48826904-48826923	MS.gene010219:CDS	45.0%
	GGAAGGACCCTTTATGGTTT+CGG	-	chr5.1:48827039-48827058	MS.gene010219:CDS	45.0%
	GGAGGAAAGGGTTTAGGAAA+GGG	-	chr5.1:48826775-48826794	MS.gene010219:CDS	45.0%
	AGGAAGAGGCAAAGGAGGAA+AGG	-	chr5.1:48826762-48826781	MS.gene010219:CDS	50.0%
	AGGACCCTTTATGGTTTCGG+TGG	-	chr5.1:48827042-48827061	MS.gene010219:CDS	50.0%
	AGGCAAGGAAGGACCCTTTA+TGG	-	chr5.1:48827033-48827052	MS.gene010219:CDS	50.0%
	ATGTCAGGAAGAGGCAAAGG+AGG	-	chr5.1:48826757-48826776	MS.gene010219:CDS	50.0%
	GGAAAGGGTTTAGGAAAGGG+CGG	-	chr5.1:48826778-48826797	MS.gene010219:CDS	50.0%
	GGAAGAGGCAAAGGAGGAAA+GGG	-	chr5.1:48826763-48826782	MS.gene010219:CDS	50.0%
	GGCAAAGGAGGAAAGGGTTT+AGG	-	chr5.1:48826769-48826788	MS.gene010219:CDS	50.0%
	GTTTATGCGCTCAAGAGGCA+AGG	-	chr5.1:48827018-48827037	MS.gene010219:CDS	50.0%
!	GGTGGTGTTAAGCGTATCAG+TGG	-	chr5.1:48826880-48826899	MS.gene010219:CDS	50.0%
	ATGCGCTCAAGAGGCAAGGA+AGG	-	chr5.1:48827022-48827041	MS.gene010219:CDS	55.0%
	TCGCAAGACGGTGACTGCTA+TGG	-	chr5.1:48826990-48827009	MS.gene010219:CDS	55.0%
	TGAACACGCTCGTCGCAAGA+CGG	-	chr5.1:48826978-48826997	MS.gene010219:CDS	55.0%
	GCGACGAGCGTGTTCAGTGT+AGG	+	chr5.1:48826974-48826993	None:intergenic	60.0%
	ATCCGTCGTCTCGCAAGGCG+TGG	-	chr5.1:48826859-48826878	MS.gene010219:CDS	65.0%
	CACCACGCCTTGCGAGACGA+CGG	+	chr5.1:48826864-48826883	None:intergenic	65.0%
	CTTGCGAGACGACGGATCGC+CGG	+	chr5.1:48826856-48826875	None:intergenic	65.0%
!	GAGACGACGGATCGCCGGTT+TGG	+	chr5.1:48826851-48826870	None:intergenic	65.0%
	CGGCGATCCGTCGTCTCGCA+AGG	-	chr5.1:48826854-48826873	MS.gene010219:CDS	70.0%
	CGTCGTCTCGCAAGGCGTGG+TGG	-	chr5.1:48826862-48826881	MS.gene010219:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	48826757	48827068	48826757	ID=MS.gene010219
chr5.1	mRNA	48826757	48827068	48826757	ID=MS.gene010219.t1;Parent=MS.gene010219
chr5.1	exon	48826757	48827068	48826757	ID=MS.gene010219.t1.exon1;Parent=MS.gene010219.t1
chr5.1	CDS	48826757	48827068	48826757	ID=cds.MS.gene010219.t1;Parent=MS.gene010219.t1

Gene Sequence	>MS.gene010219 ATGTCAGGAAGAGGCAAAGGAGGAAAGGGTTTAGGAAAGGGCGGTGCAAAGCGTCACCGCAAAGTTCTTCGAGATAACATTCAAGGTATCACCAAACCGGCGATCCGTCGTCTCGCAAGGCGTGGTGGTGTTAAGCGTATCAGTGGTCTCATCTATGAAGAAACCCGCGGAGTTCTCAAGATATTTCTCGAGAATGTGATTCGTGATGCTGTAACCTACACTGAACACGCTCGTCGCAAGACGGTGACTGCTATGGATGTTGTTTATGCGCTCAAGAGGCAAGGAAGGACCCTTTATGGTTTCGGTGGTTAG
Protein sequence	>MS.gene010219.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG