AlfalfaGEDB | MS.gene04067

M. sativa cultivar XinJiangDaYe / MS.gene04067

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04067.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04067.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04067.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene78578	MS.gene04067	PPI
MS.gene04067	MS.gene048746	PPI
MS.gene04067	MS.gene022946	PPI
MS.gene056215	MS.gene04067	PPI
MS.gene04067	MS.gene58854	PPI
MS.gene04067	MS.gene035720	PPI
MS.gene04067	MS.gene043967	PPI
MS.gene04067	MS.gene31196	PPI
MS.gene035787	MS.gene04067	PPI
MS.gene04067	MS.gene035897	PPI
MS.gene04067	MS.gene049705	PPI
MS.gene04067	MS.gene061870	PPI
MS.gene04067	MS.gene070907	PPI
MS.gene04067	MS.gene05608	PPI
MS.gene04067	MS.gene68545	PPI
MS.gene04067	MS.gene070241	PPI
MS.gene04067	MS.gene070242	PPI
MS.gene04067	MS.gene88681	PPI
MS.gene04067	MS.gene82546	PPI
MS.gene04067	MS.gene60093	PPI
MS.gene04067	MS.gene71215	PPI
MS.gene070241	MS.gene04067	PPI
MS.gene04067	MS.gene08122	PPI
MS.gene04067	MS.gene09873	PPI
MS.gene04067	MS.gene028629	PPI
MS.gene04067	MS.gene035718	PPI
MS.gene04067	MS.gene022932	PPI
MS.gene061876	MS.gene04067	PPI
MS.gene04067	MS.gene010220	PPI
MS.gene012391	MS.gene04067	PPI
MS.gene04067	MS.gene62520	PPI
MS.gene04067	MS.gene060465	PPI
MS.gene04067	MS.gene08053	PPI
MS.gene04067	MS.gene018578	PPI
MS.gene04067	MS.gene035721	PPI
MS.gene060463	MS.gene04067	PPI
MS.gene04067	MS.gene018580	PPI
MS.gene04067	MS.gene004124	PPI
MS.gene04067	MS.gene071136	PPI
MS.gene04067	MS.gene88680	PPI
MS.gene04067	MS.gene072493	PPI
MS.gene04067	MS.gene04052	PPI
MS.gene04067	MS.gene84536	PPI
MS.gene04067	MS.gene035896	PPI
MS.gene04067	MS.gene061866	PPI
MS.gene04067	MS.gene64650	PPI
MS.gene04067	MS.gene79195	PPI
MS.gene04067	MS.gene048705	PPI
MS.gene04067	MS.gene061874	PPI
MS.gene04067	MS.gene061869	PPI
MS.gene04067	MS.gene04972	PPI
MS.gene04067	MS.gene070244	PPI
MS.gene04067	MS.gene004121	PPI
MS.gene04067	MS.gene009027	PPI
MS.gene04067	MS.gene061867	PPI
MS.gene04067	MS.gene04064	PPI
MS.gene04067	MS.gene008276	PPI
MS.gene04067	MS.gene30920	PPI
MS.gene04067	MS.gene012391	PPI
MS.gene04067	MS.gene05619	PPI
MS.gene04067	MS.gene73136	PPI
MS.gene04067	MS.gene049821	PPI
MS.gene04067	MS.gene78578	PPI
MS.gene04067	MS.gene04068	PPI
MS.gene04067	MS.gene035719	PPI
MS.gene04067	MS.gene05623	PPI
MS.gene04067	MS.gene061872	PPI
MS.gene04067	MS.gene89488	PPI
MS.gene04067	MS.gene66225	PPI
MS.gene04067	MS.gene70955	PPI
MS.gene04067	MS.gene61017	PPI
MS.gene04067	MS.gene061876	PPI
MS.gene04067	MS.gene04576	PPI
MS.gene04067	MS.gene09871	PPI
MS.gene04067	MS.gene056215	PPI
MS.gene04067	MS.gene012390	PPI
MS.gene04067	MS.gene08121	PPI
MS.gene04067	MS.gene64920	PPI
MS.gene71215	MS.gene04067	PPI
MS.gene04067	MS.gene064557	PPI
MS.gene04067	MS.gene035787	PPI
MS.gene04067	MS.gene04434	PPI
MS.gene04067	MS.gene04452	PPI
MS.gene04067	MS.gene04392	PPI
MS.gene04067	MS.gene060463	PPI
MS.gene04067	MS.gene89487	PPI
MS.gene04067	MS.gene010219	PPI
MS.gene04067	MS.gene04433	PPI
MS.gene04067	MS.gene040383	PPI
MS.gene04067	MS.gene038469	PPI
MS.gene071136	MS.gene04067	PPI
MS.gene04067	MS.gene016624	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04067.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04067.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04067.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04067.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04067.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04067.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04067.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04067.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04067.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04067.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04067.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04067.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04067.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04067.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04067.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene04067.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04067.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04067.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04067.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04067.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04067.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04067.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04067.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04067.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04067.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04067.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04067.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 32 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGTGTATTGAAGATATTCT+TGG	0.398568	3.1:-46705727	MS.gene04067:CDS
AAAATGTCAGGTCGTGGAAA+GGG	0.434071	3.1:-46705897	MS.gene04067:CDS
AGACAGGGAAGAACGCTCTA+TGG	0.434419	3.1:-46705618	MS.gene04067:CDS
GGAAGAACGCTCTATGGTTT+CGG	0.438131	3.1:-46705612	MS.gene04067:CDS
CTCGCCAATCGGCGAATAGC+AGG	0.453484	3.1:+46705798	None:intergenic
TGTCTATGCTTTGAAGAGAC+AGG	0.458633	3.1:-46705634	MS.gene04067:CDS
GGTAAGGGTCTGGGAAAGGG+AGG	0.476615	3.1:-46705873	MS.gene04067:CDS
GAATATCTTCAATACACCTC+TGG	0.487673	3.1:+46705731	None:intergenic
CACCACCTCTTCTCGCCAAT+CGG	0.499060	3.1:+46705787	None:intergenic
GGTTCTTCGTGACAACATCC+AGG	0.501637	3.1:-46705832	MS.gene04067:CDS
CAAGCCTGCTATTCGCCGAT+TGG	0.502152	3.1:-46705802	MS.gene04067:CDS
CTCCCGAAAATGTCAGGTCG+TGG	0.510898	3.1:-46705903	None:intergenic
CAATCGGCGAATAGCAGGCT+TGG	0.515998	3.1:+46705803	None:intergenic
AGGGAGGTGCAAAGAGACAT+AGG	0.520046	3.1:-46705857	MS.gene04067:CDS
CGCCGATTGGCGAGAAGAGG+TGG	0.530277	3.1:-46705789	MS.gene04067:CDS
AAATGTCAGGTCGTGGAAAG+GGG	0.537019	3.1:-46705896	MS.gene04067:CDS
GGTGGTGTGAAGAGAATCAG+TGG	0.556654	3.1:-46705771	MS.gene04067:CDS
GAGGAAGACGGTGACAGCCA+TGG	0.561434	3.1:-46705661	MS.gene04067:CDS
GTCTATGCTTTGAAGAGACA+GGG	0.584038	3.1:-46705633	MS.gene04067:CDS
AATGTCAGGTCGTGGAAAGG+GGG	0.584231	3.1:-46705895	MS.gene04067:CDS
TGACATACACTGAGCACGCA+AGG	0.587222	3.1:-46705683	MS.gene04067:CDS
GAAAATGTCAGGTCGTGGAA+AGG	0.589881	3.1:-46705898	None:intergenic
TAGCAGGCTTGGTGATACCC+TGG	0.594313	3.1:+46705814	None:intergenic
ATTCGCCGATTGGCGAGAAG+AGG	0.615284	3.1:-46705792	MS.gene04067:CDS
ATGTCAGGTCGTGGAAAGGG+GGG	0.615792	3.1:-46705894	MS.gene04067:CDS
AGGTGCAAAGAGACATAGGA+AGG	0.617656	3.1:-46705853	MS.gene04067:CDS
AGAACGCTCTATGGTTTCGG+AGG	0.628880	3.1:-46705609	MS.gene04067:CDS
CAAAGCATAGACAACATCCA+TGG	0.629842	3.1:+46705644	None:intergenic
TGAGCACGCAAGGAGGAAGA+CGG	0.652638	3.1:-46705673	MS.gene04067:CDS
GTTCTTCGTGACAACATCCA+GGG	0.676380	3.1:-46705831	MS.gene04067:CDS
TTGATATATGAAGAAACCAG+AGG	0.683025	3.1:-46705747	MS.gene04067:CDS
CATACACTGAGCACGCAAGG+AGG	0.730442	3.1:-46705680	MS.gene04067:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGGTGTATTGAAGATATTCT+TGG	-	chr3.1:46705772-46705791	MS.gene04067:CDS	30.0%
	TTGATATATGAAGAAACCAG+AGG	-	chr3.1:46705752-46705771	MS.gene04067:CDS	30.0%
	GAATATCTTCAATACACCTC+TGG	+	chr3.1:46705771-46705790	None:intergenic	35.0%
	CAAAGCATAGACAACATCCA+TGG	+	chr3.1:46705858-46705877	None:intergenic	40.0%
!!	GTCTATGCTTTGAAGAGACA+GGG	-	chr3.1:46705866-46705885	MS.gene04067:CDS	40.0%
!!	TGTCTATGCTTTGAAGAGAC+AGG	-	chr3.1:46705865-46705884	MS.gene04067:CDS	40.0%
	AGGTGCAAAGAGACATAGGA+AGG	-	chr3.1:46705646-46705665	MS.gene04067:CDS	45.0%
	GGAAGAACGCTCTATGGTTT+CGG	-	chr3.1:46705887-46705906	MS.gene04067:CDS	45.0%
	GTTCTTCGTGACAACATCCA+GGG	-	chr3.1:46705668-46705687	MS.gene04067:CDS	45.0%
	AGAACGCTCTATGGTTTCGG+AGG	-	chr3.1:46705890-46705909	MS.gene04067:CDS	50.0%
	AGACAGGGAAGAACGCTCTA+TGG	-	chr3.1:46705881-46705900	MS.gene04067:CDS	50.0%
	GGTGGTGTGAAGAGAATCAG+TGG	-	chr3.1:46705728-46705747	MS.gene04067:CDS	50.0%
	GGTTCTTCGTGACAACATCC+AGG	-	chr3.1:46705667-46705686	MS.gene04067:CDS	50.0%
	TGACATACACTGAGCACGCA+AGG	-	chr3.1:46705816-46705835	MS.gene04067:CDS	50.0%
!	AGGGAGGTGCAAAGAGACAT+AGG	-	chr3.1:46705642-46705661	MS.gene04067:CDS	50.0%
	ATGTCAGGTCGTGGAAAGGG+GGG	-	chr3.1:46705605-46705624	MS.gene04067:CDS	55.0%
	CAAGCCTGCTATTCGCCGAT+TGG	-	chr3.1:46705697-46705716	MS.gene04067:CDS	55.0%
	CAATCGGCGAATAGCAGGCT+TGG	+	chr3.1:46705699-46705718	None:intergenic	55.0%
	CACCACCTCTTCTCGCCAAT+CGG	+	chr3.1:46705715-46705734	None:intergenic	55.0%
	CATACACTGAGCACGCAAGG+AGG	-	chr3.1:46705819-46705838	MS.gene04067:CDS	55.0%
	TGAGCACGCAAGGAGGAAGA+CGG	-	chr3.1:46705826-46705845	MS.gene04067:CDS	55.0%
!	ATTCGCCGATTGGCGAGAAG+AGG	-	chr3.1:46705707-46705726	MS.gene04067:CDS	55.0%
!!	TAGCAGGCTTGGTGATACCC+TGG	+	chr3.1:46705688-46705707	None:intergenic	55.0%
	AGGTCGTGGAAAGGGGGGTA+AGG	-	chr3.1:46705610-46705629	MS.gene04067:CDS	60.0%
	CTCGCCAATCGGCGAATAGC+AGG	+	chr3.1:46705704-46705723	None:intergenic	60.0%
	GAGGAAGACGGTGACAGCCA+TGG	-	chr3.1:46705838-46705857	MS.gene04067:CDS	60.0%
	GGAAAGGGGGGTAAGGGTCT+GGG	-	chr3.1:46705617-46705636	MS.gene04067:CDS	60.0%
	GGGGGTAAGGGTCTGGGAAA+GGG	-	chr3.1:46705623-46705642	MS.gene04067:CDS	60.0%
	GGTAAGGGTCTGGGAAAGGG+AGG	-	chr3.1:46705626-46705645	MS.gene04067:CDS	60.0%
	GGTCGTGGAAAGGGGGGTAA+GGG	-	chr3.1:46705611-46705630	MS.gene04067:CDS	60.0%
	TGGAAAGGGGGGTAAGGGTC+TGG	-	chr3.1:46705616-46705635	MS.gene04067:CDS	60.0%
	GGGGGGTAAGGGTCTGGGAA+AGG	-	chr3.1:46705622-46705641	MS.gene04067:CDS	65.0%
!	CGCCGATTGGCGAGAAGAGG+TGG	-	chr3.1:46705710-46705729	MS.gene04067:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	46705605	46705916	46705605	ID=MS.gene04067
chr3.1	mRNA	46705605	46705916	46705605	ID=MS.gene04067.t1;Parent=MS.gene04067
chr3.1	exon	46705605	46705916	46705605	ID=MS.gene04067.t1.exon1;Parent=MS.gene04067.t1
chr3.1	CDS	46705605	46705916	46705605	ID=cds.MS.gene04067.t1;Parent=MS.gene04067.t1

Gene Sequence	>MS.gene04067 ATGTCAGGTCGTGGAAAGGGGGGTAAGGGTCTGGGAAAGGGAGGTGCAAAGAGACATAGGAAGGTTCTTCGTGACAACATCCAGGGTATCACCAAGCCTGCTATTCGCCGATTGGCGAGAAGAGGTGGTGTGAAGAGAATCAGTGGATTGATATATGAAGAAACCAGAGGTGTATTGAAGATATTCTTGGAAAACGTCATTCGTGATGCTGTGACATACACTGAGCACGCAAGGAGGAAGACGGTGACAGCCATGGATGTTGTCTATGCTTTGAAGAGACAGGGAAGAACGCTCTATGGTTTCGGAGGTTGA
Protein sequence	>MS.gene04067.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG