AlfalfaGEDB | MS.gene61017

M. sativa cultivar XinJiangDaYe / MS.gene61017

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61017.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61017.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61017.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene61017	0.803309	3.71E-49	-1.69E-46
MS.gene049341	MS.gene61017	0.800653	1.30E-48	-1.69E-46
MS.gene049821	MS.gene61017	0.832198	1.08E-55	-1.69E-46
MS.gene050020	MS.gene61017	0.825866	3.71E-54	-1.69E-46
MS.gene050689	MS.gene61017	0.801219	9.98E-49	-1.69E-46
MS.gene050807	MS.gene61017	0.851282	9.82E-61	-1.69E-46
MS.gene050808	MS.gene61017	0.832834	7.54E-56	-1.69E-46
MS.gene050901	MS.gene61017	0.839416	1.61E-57	-1.69E-46
MS.gene051247	MS.gene61017	0.81987	9.26E-53	-1.69E-46
MS.gene051328	MS.gene61017	0.825354	4.91E-54	-1.69E-46
MS.gene051719	MS.gene61017	0.811446	6.97E-51	-1.69E-46
MS.gene052133	MS.gene61017	0.821181	4.63E-53	-1.69E-46
MS.gene052516	MS.gene61017	0.803693	3.09E-49	-1.69E-46
MS.gene052947	MS.gene61017	0.818836	1.59E-52	-1.69E-46
MS.gene053226	MS.gene61017	0.81054	1.10E-50	-1.69E-46
MS.gene053563	MS.gene61017	0.85137	9.27E-61	-1.69E-46
MS.gene053581	MS.gene61017	0.839651	1.40E-57	-1.69E-46
MS.gene05364	MS.gene61017	0.823039	1.72E-53	-1.69E-46
MS.gene055466	MS.gene61017	0.808007	3.83E-50	-1.69E-46
MS.gene055512	MS.gene61017	0.805024	1.63E-49	-1.69E-46
MS.gene056005	MS.gene61017	0.800898	1.16E-48	-1.69E-46
MS.gene056215	MS.gene61017	0.802503	5.44E-49	-1.69E-46
MS.gene056760	MS.gene61017	0.808268	3.37E-50	-1.69E-46
MS.gene057060	MS.gene61017	0.808288	3.33E-50	-1.69E-46
MS.gene057693	MS.gene61017	0.810358	1.20E-50	-1.69E-46
MS.gene057694	MS.gene61017	0.816331	5.84E-52	-1.69E-46
MS.gene057705	MS.gene61017	0.830572	2.72E-55	-1.69E-46
MS.gene058852	MS.gene61017	0.804377	2.22E-49	-1.69E-46
MS.gene060429	MS.gene61017	0.806694	7.26E-50	-1.69E-46
MS.gene060587	MS.gene61017	0.824578	7.48E-54	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene056215	MS.gene61017	PPI
MS.gene064557	MS.gene61017	PPI
MS.gene62520	MS.gene61017	PPI
MS.gene038469	MS.gene61017	PPI
MS.gene61017	MS.gene035720	PPI
MS.gene04452	MS.gene61017	PPI
MS.gene61017	MS.gene012390	PPI
MS.gene035787	MS.gene61017	PPI
MS.gene09871	MS.gene61017	PPI
MS.gene04576	MS.gene61017	PPI
MS.gene61017	MS.gene049705	PPI
MS.gene89487	MS.gene61017	PPI
MS.gene61017	MS.gene010219	PPI
MS.gene58854	MS.gene61017	PPI
MS.gene61017	MS.gene05608	PPI
MS.gene61017	MS.gene68545	PPI
MS.gene04392	MS.gene61017	PPI
MS.gene072493	MS.gene61017	PPI
MS.gene035720	MS.gene61017	PPI
MS.gene30920	MS.gene61017	PPI
MS.gene61017	MS.gene016624	PPI
MS.gene048746	MS.gene61017	PPI
MS.gene010220	MS.gene61017	PPI
MS.gene08053	MS.gene61017	PPI
MS.gene061872	MS.gene61017	PPI
MS.gene88680	MS.gene61017	PPI
MS.gene070241	MS.gene61017	PPI
MS.gene04434	MS.gene61017	PPI
MS.gene049821	MS.gene61017	PPI
MS.gene022946	MS.gene61017	PPI
MS.gene61017	MS.gene09873	PPI
MS.gene04064	MS.gene61017	PPI
MS.gene061876	MS.gene61017	PPI
MS.gene05608	MS.gene61017	PPI
MS.gene012391	MS.gene61017	PPI
MS.gene88681	MS.gene61017	PPI
MS.gene070244	MS.gene61017	PPI
MS.gene035897	MS.gene61017	PPI
MS.gene64920	MS.gene61017	PPI
MS.gene060463	MS.gene61017	PPI
MS.gene31196	MS.gene61017	PPI
MS.gene010219	MS.gene61017	PPI
MS.gene060465	MS.gene61017	PPI
MS.gene61017	MS.gene84536	PPI
MS.gene04068	MS.gene61017	PPI
MS.gene61017	MS.gene64650	PPI
MS.gene61017	MS.gene60093	PPI
MS.gene61017	MS.gene061869	PPI
MS.gene08121	MS.gene61017	PPI
MS.gene022932	MS.gene61017	PPI
MS.gene61017	MS.gene04972	PPI
MS.gene61017	MS.gene004121	PPI
MS.gene035718	MS.gene61017	PPI
MS.gene61017	MS.gene62520	PPI
MS.gene035896	MS.gene61017	PPI
MS.gene73136	MS.gene61017	PPI
MS.gene008276	MS.gene61017	PPI
MS.gene61017	MS.gene018578	PPI
MS.gene070907	MS.gene61017	PPI
MS.gene61017	MS.gene061867	PPI
MS.gene009027	MS.gene61017	PPI
MS.gene61017	MS.gene018580	PPI
MS.gene61017	MS.gene004124	PPI
MS.gene09873	MS.gene61017	PPI
MS.gene78578	MS.gene61017	PPI
MS.gene61017	MS.gene05619	PPI
MS.gene035721	MS.gene61017	PPI
MS.gene04052	MS.gene61017	PPI
MS.gene08122	MS.gene61017	PPI
MS.gene048705	MS.gene61017	PPI
MS.gene05623	MS.gene61017	PPI
MS.gene61017	MS.gene04052	PPI
MS.gene043967	MS.gene61017	PPI
MS.gene61017	MS.gene89488	PPI
MS.gene61017	MS.gene061866	PPI
MS.gene84536	MS.gene61017	PPI
MS.gene028629	MS.gene61017	PPI
MS.gene82546	MS.gene61017	PPI
MS.gene61017	MS.gene048705	PPI
MS.gene04433	MS.gene61017	PPI
MS.gene61017	MS.gene061874	PPI
MS.gene61017	MS.gene04576	PPI
MS.gene04067	MS.gene61017	PPI
MS.gene71215	MS.gene61017	PPI
MS.gene061866	MS.gene61017	PPI
MS.gene061870	MS.gene61017	PPI
MS.gene035719	MS.gene61017	PPI
MS.gene66225	MS.gene61017	PPI
MS.gene70955	MS.gene61017	PPI
MS.gene071136	MS.gene61017	PPI
MS.gene040383	MS.gene61017	PPI
MS.gene070242	MS.gene61017	PPI
MS.gene012390	MS.gene61017	PPI
MS.gene61017	MS.gene008276	PPI
MS.gene61017	MS.gene73136	PPI
MS.gene79195	MS.gene61017	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61017.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene61017.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene61017.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene61017.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene61017.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene61017.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene61017.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene61017.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene61017.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene61017.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene61017.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene61017.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene61017.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene61017.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene61017.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene61017.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61017.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene61017.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene61017.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene61017.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene61017.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene61017.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene61017.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene61017.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene61017.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene61017.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene61017.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 30 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCCTGCGATTCGTCGTT+TGG	0.336158	8.1:-37973165	MS.gene61017:CDS
GGGAGAACTCTTTACGGTTT+CGG	0.376970	8.1:-37972975	MS.gene61017:CDS
TCGCAGGCTTTGTTATTCCT+TGG	0.407291	8.1:+37973177	None:intergenic
AAAATGTCAGGTCGCGGAAA+AGG	0.432832	8.1:-37973260	MS.gene61017:CDS
AGACAGGGGAGAACTCTTTA+CGG	0.442868	8.1:-37972981	MS.gene61017:CDS
GGAAAAGGAGGAAAAGGATT+AGG	0.458349	8.1:-37973245	MS.gene61017:CDS
TGTTTATGCTTTGAAGAGAC+AGG	0.461410	8.1:-37972997	MS.gene61017:CDS
AGGAGGAAAAGGATTAGGAA+AGG	0.511643	8.1:-37973240	MS.gene61017:CDS
AACAACATCCATGGCGGTAA+CGG	0.516175	8.1:+37973016	None:intergenic
CGTCGTTTGGCTCGAAGAGG+AGG	0.516708	8.1:-37973152	MS.gene61017:CDS
GGTCGCGGAAAAGGAGGAAA+AGG	0.530282	8.1:-37973251	MS.gene61017:CDS
GGAGGAAAAGGATTAGGAAA+GGG	0.534282	8.1:-37973239	MS.gene61017:CDS
TTACTTACACTGAGCACGCT+AGG	0.551062	8.1:-37973046	MS.gene61017:CDS
CGAGCCAAACGACGAATCGC+AGG	0.562444	8.1:+37973161	None:intergenic
GAGGAAAACCGTTACCGCCA+TGG	0.565591	8.1:-37973024	MS.gene61017:CDS
AGGAGCGAAGAGGCACAGAA+AGG	0.576961	8.1:-37973216	MS.gene61017:CDS
GGAAAAGGATTAGGAAAGGG+AGG	0.577550	8.1:-37973236	MS.gene61017:CDS
ATGTCAGGTCGCGGAAAAGG+AGG	0.578066	8.1:-37973257	MS.gene61017:CDS
GTTCTTCGTGATAACATCCA+AGG	0.589462	8.1:-37973194	MS.gene61017:CDS
GTTTATGCTTTGAAGAGACA+GGG	0.590468	8.1:-37972996	MS.gene61017:CDS
GGAGGTGTGAAGAGAATCAG+TGG	0.594114	8.1:-37973134	MS.gene61017:CDS
AGAACTCTTTACGGTTTCGG+TGG	0.599890	8.1:-37972972	MS.gene61017:CDS
CAAAGCATAAACAACATCCA+TGG	0.612707	8.1:+37973007	None:intergenic
ATTCGTCGTTTGGCTCGAAG+AGG	0.614299	8.1:-37973155	MS.gene61017:CDS
CTTACACTGAGCACGCTAGG+AGG	0.661001	8.1:-37973043	MS.gene61017:CDS
TTTATGCTTTGAAGAGACAG+GGG	0.664122	8.1:-37972995	MS.gene61017:CDS
TAGGAAAGGGAGGAGCGAAG+AGG	0.669088	8.1:-37973226	MS.gene61017:CDS
CTTATCTATGAAGAAACTCG+CGG	0.683519	8.1:-37973110	MS.gene61017:CDS
CATCACGAATAACGTTCTCG+AGG	0.697048	8.1:+37973072	None:intergenic
AGCATAAACAACATCCATGG+CGG	0.725411	8.1:+37973010	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CAAAGCATAAACAACATCCA+TGG	+	chr8.1:37973221-37973240	None:intergenic	35.0%
	CTTATCTATGAAGAAACTCG+CGG	-	chr8.1:37973115-37973134	MS.gene61017:CDS	35.0%
!!	GTTTATGCTTTGAAGAGACA+GGG	-	chr8.1:37973229-37973248	MS.gene61017:CDS	35.0%
!!	TGTTTATGCTTTGAAGAGAC+AGG	-	chr8.1:37973228-37973247	MS.gene61017:CDS	35.0%
!!	TTTATGCTTTGAAGAGACAG+GGG	-	chr8.1:37973230-37973249	MS.gene61017:CDS	35.0%
	AGCATAAACAACATCCATGG+CGG	+	chr8.1:37973218-37973237	None:intergenic	40.0%
	AGGAGGAAAAGGATTAGGAA+AGG	-	chr8.1:37972985-37973004	MS.gene61017:CDS	40.0%
	GGAAAAGGAGGAAAAGGATT+AGG	-	chr8.1:37972980-37972999	MS.gene61017:CDS	40.0%
	GGAGGAAAAGGATTAGGAAA+GGG	-	chr8.1:37972986-37973005	MS.gene61017:CDS	40.0%
	GTTCTTCGTGATAACATCCA+AGG	-	chr8.1:37973031-37973050	MS.gene61017:CDS	40.0%
	AACAACATCCATGGCGGTAA+CGG	+	chr8.1:37973212-37973231	None:intergenic	45.0%
	AGAACTCTTTACGGTTTCGG+TGG	-	chr8.1:37973253-37973272	MS.gene61017:CDS	45.0%
	AGACAGGGGAGAACTCTTTA+CGG	-	chr8.1:37973244-37973263	MS.gene61017:CDS	45.0%
	CATCACGAATAACGTTCTCG+AGG	+	chr8.1:37973156-37973175	None:intergenic	45.0%
	GGAAAAGGATTAGGAAAGGG+AGG	-	chr8.1:37972989-37973008	MS.gene61017:CDS	45.0%
	GGGAGAACTCTTTACGGTTT+CGG	-	chr8.1:37973250-37973269	MS.gene61017:CDS	45.0%
	TTACTTACACTGAGCACGCT+AGG	-	chr8.1:37973179-37973198	MS.gene61017:CDS	45.0%
!!	TCGCAGGCTTTGTTATTCCT+TGG	+	chr8.1:37973051-37973070	None:intergenic	45.0%
	AAAGCCTGCGATTCGTCGTT+TGG	-	chr8.1:37973060-37973079	MS.gene61017:CDS	50.0%
!	ATTCGTCGTTTGGCTCGAAG+AGG	-	chr8.1:37973070-37973089	MS.gene61017:CDS	50.0%
!	GGAGGTGTGAAGAGAATCAG+TGG	-	chr8.1:37973091-37973110	MS.gene61017:CDS	50.0%
	ATGTCAGGTCGCGGAAAAGG+AGG	-	chr8.1:37972968-37972987	MS.gene61017:CDS	55.0%
	CTTACACTGAGCACGCTAGG+AGG	-	chr8.1:37973182-37973201	MS.gene61017:CDS	55.0%
	GAGGAAAACCGTTACCGCCA+TGG	-	chr8.1:37973201-37973220	MS.gene61017:CDS	55.0%
	GGTCGCGGAAAAGGAGGAAA+AGG	-	chr8.1:37972974-37972993	MS.gene61017:CDS	55.0%
	TAGGAAAGGGAGGAGCGAAG+AGG	-	chr8.1:37972999-37973018	MS.gene61017:CDS	55.0%
!	AGGAGCGAAGAGGCACAGAA+AGG	-	chr8.1:37973009-37973028	MS.gene61017:CDS	55.0%
	CGAGCCAAACGACGAATCGC+AGG	+	chr8.1:37973067-37973086	None:intergenic	60.0%
!	CGTCGTTTGGCTCGAAGAGG+AGG	-	chr8.1:37973073-37973092	MS.gene61017:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	37972968	37973279	37972968	ID=MS.gene61017
chr8.1	mRNA	37972968	37973279	37972968	ID=MS.gene61017.t1;Parent=MS.gene61017
chr8.1	exon	37972968	37973279	37972968	ID=MS.gene61017.t1.exon1;Parent=MS.gene61017.t1
chr8.1	CDS	37972968	37973279	37972968	ID=cds.MS.gene61017.t1;Parent=MS.gene61017.t1

Gene Sequence	>MS.gene61017 ATGTCAGGTCGCGGAAAAGGAGGAAAAGGATTAGGAAAGGGAGGAGCGAAGAGGCACAGAAAGGTTCTTCGTGATAACATCCAAGGAATAACAAAGCCTGCGATTCGTCGTTTGGCTCGAAGAGGAGGTGTGAAGAGAATCAGTGGTCTTATCTATGAAGAAACTCGCGGTGTTCTCAAGATCTTCCTCGAGAACGTTATTCGTGATGCTGTTACTTACACTGAGCACGCTAGGAGGAAAACCGTTACCGCCATGGATGTTGTTTATGCTTTGAAGAGACAGGGGAGAACTCTTTACGGTTTCGGTGGTTGA
Protein sequence	>MS.gene61017.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG