AlfalfaGEDB | MS.gene70955

M. sativa cultivar XinJiangDaYe / MS.gene70955

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70955.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70955.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70955.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049821	MS.gene70955	0.85259	4.18E-61	-1.69E-46
MS.gene050901	MS.gene70955	0.851758	7.20E-61	-1.69E-46
MS.gene051247	MS.gene70955	0.802813	4.70E-49	-1.69E-46
MS.gene05157	MS.gene70955	0.820805	5.65E-53	-1.69E-46
MS.gene051664	MS.gene70955	0.83166	1.47E-55	-1.69E-46
MS.gene051700	MS.gene70955	0.805302	1.43E-49	-1.69E-46
MS.gene051719	MS.gene70955	0.811464	6.91E-51	-1.69E-46
MS.gene051720	MS.gene70955	0.824458	7.98E-54	-1.69E-46
MS.gene052133	MS.gene70955	0.81088	9.25E-51	-1.69E-46
MS.gene053226	MS.gene70955	0.809104	2.23E-50	-1.69E-46
MS.gene053581	MS.gene70955	0.830674	2.57E-55	-1.69E-46
MS.gene055465	MS.gene70955	0.839086	1.96E-57	-1.69E-46
MS.gene055466	MS.gene70955	0.837996	3.74E-57	-1.69E-46
MS.gene055468	MS.gene70955	0.81198	5.34E-51	-1.69E-46
MS.gene055469	MS.gene70955	0.809799	1.58E-50	-1.69E-46
MS.gene055537	MS.gene70955	0.818089	2.35E-52	-1.69E-46
MS.gene05608	MS.gene70955	0.82219	2.70E-53	-1.69E-46
MS.gene056169	MS.gene70955	0.812731	3.66E-51	-1.69E-46
MS.gene056215	MS.gene70955	0.861418	1.04E-63	-1.69E-46
MS.gene057294	MS.gene70955	0.812035	5.19E-51	-1.69E-46
MS.gene059625	MS.gene70955	0.805046	1.61E-49	-1.69E-46
MS.gene060429	MS.gene70955	0.81138	7.21E-51	-1.69E-46
MS.gene060519	MS.gene70955	0.813611	2.34E-51	-1.69E-46
MS.gene060587	MS.gene70955	0.813338	2.69E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene70955	MS.gene04452	PPI
MS.gene056215	MS.gene70955	PPI
MS.gene064557	MS.gene70955	PPI
MS.gene038469	MS.gene70955	PPI
MS.gene04433	MS.gene70955	PPI
MS.gene04452	MS.gene70955	PPI
MS.gene70955	MS.gene016624	PPI
MS.gene70955	MS.gene008276	PPI
MS.gene035787	MS.gene70955	PPI
MS.gene70955	MS.gene73136	PPI
MS.gene70955	MS.gene049821	PPI
MS.gene89487	MS.gene70955	PPI
MS.gene70955	MS.gene022946	PPI
MS.gene70955	MS.gene05623	PPI
MS.gene035719	MS.gene70955	PPI
MS.gene58854	MS.gene70955	PPI
MS.gene70955	MS.gene035720	PPI
MS.gene040383	MS.gene70955	PPI
MS.gene30920	MS.gene70955	PPI
MS.gene70955	MS.gene09871	PPI
MS.gene08053	MS.gene70955	PPI
MS.gene061872	MS.gene70955	PPI
MS.gene70955	MS.gene049705	PPI
MS.gene88680	MS.gene70955	PPI
MS.gene070241	MS.gene70955	PPI
MS.gene70955	MS.gene070907	PPI
MS.gene70955	MS.gene04392	PPI
MS.gene70955	MS.gene010219	PPI
MS.gene70955	MS.gene05608	PPI
MS.gene70955	MS.gene040383	PPI
MS.gene70955	MS.gene68545	PPI
MS.gene061876	MS.gene70955	PPI
MS.gene70955	MS.gene070242	PPI
MS.gene012391	MS.gene70955	PPI
MS.gene070244	MS.gene70955	PPI
MS.gene70955	MS.gene82546	PPI
MS.gene64920	MS.gene70955	PPI
MS.gene70955	MS.gene048746	PPI
MS.gene060463	MS.gene70955	PPI
MS.gene70955	MS.gene08122	PPI
MS.gene70955	MS.gene09873	PPI
MS.gene31196	MS.gene70955	PPI
MS.gene70955	MS.gene022932	PPI
MS.gene70955	MS.gene043967	PPI
MS.gene70955	MS.gene62520	PPI
MS.gene70955	MS.gene035897	PPI
MS.gene70955	MS.gene035721	PPI
MS.gene70955	MS.gene004124	PPI
MS.gene08121	MS.gene70955	PPI
MS.gene70955	MS.gene072493	PPI
MS.gene88681	MS.gene70955	PPI
MS.gene70955	MS.gene84536	PPI
MS.gene70955	MS.gene035896	PPI
MS.gene70955	MS.gene061866	PPI
MS.gene035718	MS.gene70955	PPI
MS.gene70955	MS.gene64650	PPI
MS.gene70955	MS.gene60093	PPI
MS.gene009027	MS.gene70955	PPI
MS.gene70955	MS.gene061869	PPI
MS.gene78578	MS.gene70955	PPI
MS.gene060465	MS.gene70955	PPI
MS.gene70955	MS.gene010220	PPI
MS.gene70955	MS.gene04972	PPI
MS.gene70955	MS.gene004121	PPI
MS.gene028629	MS.gene70955	PPI
MS.gene70955	MS.gene018578	PPI
MS.gene70955	MS.gene061867	PPI
MS.gene70955	MS.gene04064	PPI
MS.gene70955	MS.gene018580	PPI
MS.gene04067	MS.gene70955	PPI
MS.gene71215	MS.gene70955	PPI
MS.gene70955	MS.gene05619	PPI
MS.gene061870	MS.gene70955	PPI
MS.gene70955	MS.gene04068	PPI
MS.gene70955	MS.gene04052	PPI
MS.gene70955	MS.gene89488	PPI
MS.gene70955	MS.gene66225	PPI
MS.gene70955	MS.gene79195	PPI
MS.gene70955	MS.gene048705	PPI
MS.gene70955	MS.gene61017	PPI
MS.gene071136	MS.gene70955	PPI
MS.gene70955	MS.gene061874	PPI
MS.gene70955	MS.gene04576	PPI
MS.gene70955	MS.gene012390	PPI
MS.gene70955	MS.gene08121	PPI
MS.gene70955	MS.gene064557	PPI
MS.gene70955	MS.gene04434	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70955.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene70955.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene70955.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene70955.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene70955.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene70955.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene70955.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene70955.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene70955.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene70955.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene70955.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene70955.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene70955.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene70955.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene70955.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene70955.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70955.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene70955.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene70955.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene70955.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene70955.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene70955.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene70955.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene70955.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene70955.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene70955.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene70955.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 29 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGACAAGGAAGAACGCTTTA+TGG	0.218720	3.3:-44574989	MS.gene70955:CDS
CTTGCCAATCGACGAATTGC+TGG	0.313129	3.3:+44575169	None:intergenic
GGAAGAACGCTTTATGGATT+CGG	0.427609	3.3:-44574983	MS.gene70955:CDS
GGGAGGTAAGGGACTAGGAA+AGG	0.428277	3.3:-44575248	MS.gene70955:CDS
GGAGGTAAGGGACTAGGAAA+GGG	0.452874	3.3:-44575247	MS.gene70955:CDS
CTCTTGAAAATGTCAGGTCG+TGG	0.456977	3.3:-44575274	None:intergenic
AGGTCGTGGAAAGGGAGGTA+AGG	0.492819	3.3:-44575260	MS.gene70955:CDS
AGTTCTTCGTGACAACATCC+AGG	0.501637	3.3:-44575203	MS.gene70955:CDS
CAATCGACGAATTGCTGGCT+TGG	0.513777	3.3:+44575174	None:intergenic
CAAGCCAGCAATTCGTCGAT+TGG	0.518519	3.3:-44575173	MS.gene70955:CDS
GGAAAGGGAGGTAAGGGACT+AGG	0.521051	3.3:-44575253	MS.gene70955:CDS
AAAATGTCAGGTCGTGGAAA+GGG	0.538992	3.3:-44575268	MS.gene70955:CDS
GAGGAAGACAGTGACTGCTA+TGG	0.554386	3.3:-44575032	MS.gene70955:CDS
GGTAAGGGACTAGGAAAGGG+AGG	0.563878	3.3:-44575244	MS.gene70955:CDS
GGTCGTGGAAAGGGAGGTAA+GGG	0.564245	3.3:-44575259	MS.gene70955:CDS
AATCAGCGGCTTGATCTATG+AGG	0.573064	3.3:-44575128	MS.gene70955:CDS
TTGCTGGCTTGGTGATACCC+TGG	0.584362	3.3:+44575185	None:intergenic
GAAAATGTCAGGTCGTGGAA+AGG	0.584382	3.3:-44575269	None:intergenic
CGTCGATTGGCAAGAAGAGG+TGG	0.589209	3.3:-44575160	MS.gene70955:CDS
TGACATACACTGAGCATGCA+AGG	0.589705	3.3:-44575054	MS.gene70955:CDS
AGAACGCTTTATGGATTCGG+AGG	0.604365	3.3:-44574980	MS.gene70955:CDS
ATTCGTCGATTGGCAAGAAG+AGG	0.627130	3.3:-44575163	MS.gene70955:CDS
GTTTATGCTTTGAAGAGACA+AGG	0.627217	3.3:-44575004	MS.gene70955:CDS
ATGTCAGGTCGTGGAAAGGG+AGG	0.643397	3.3:-44575265	MS.gene70955:CDS
TTGATCTATGAGGAAACAAG+AGG	0.653373	3.3:-44575118	MS.gene70955:CDS
GTTCTTCGTGACAACATCCA+GGG	0.666413	3.3:-44575202	MS.gene70955:CDS
GGTGGTGTTAAGAGAATCAG+CGG	0.669368	3.3:-44575142	MS.gene70955:CDS
GGAGAATGTGATTCGTGACG+CGG	0.710348	3.3:-44575077	MS.gene70955:CDS
CATACACTGAGCATGCAAGG+AGG	0.756152	3.3:-44575051	MS.gene70955:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGGTGTTCTTAAGATTTTCT+TGG	-	chr3.3:44575143-44575162	MS.gene70955:CDS	30.0%
	TTGATCTATGAGGAAACAAG+AGG	-	chr3.3:44575123-44575142	MS.gene70955:CDS	35.0%
!!	GTTTATGCTTTGAAGAGACA+AGG	-	chr3.3:44575237-44575256	MS.gene70955:CDS	35.0%
	AGACAAGGAAGAACGCTTTA+TGG	-	chr3.3:44575252-44575271	MS.gene70955:CDS	40.0%
	GGAAGAACGCTTTATGGATT+CGG	-	chr3.3:44575258-44575277	MS.gene70955:CDS	40.0%
	AATCAGCGGCTTGATCTATG+AGG	-	chr3.3:44575113-44575132	MS.gene70955:CDS	45.0%
	AGAACGCTTTATGGATTCGG+AGG	-	chr3.3:44575261-44575280	MS.gene70955:CDS	45.0%
	AGTTCTTCGTGACAACATCC+AGG	-	chr3.3:44575038-44575057	MS.gene70955:CDS	45.0%
	GTTCTTCGTGACAACATCCA+GGG	-	chr3.3:44575039-44575058	MS.gene70955:CDS	45.0%
	TGACATACACTGAGCATGCA+AGG	-	chr3.3:44575187-44575206	MS.gene70955:CDS	45.0%
!	ATTCGTCGATTGGCAAGAAG+AGG	-	chr3.3:44575078-44575097	MS.gene70955:CDS	45.0%
!	GGTGGTGTTAAGAGAATCAG+CGG	-	chr3.3:44575099-44575118	MS.gene70955:CDS	45.0%
	CAAGCCAGCAATTCGTCGAT+TGG	-	chr3.3:44575068-44575087	MS.gene70955:CDS	50.0%
	CATACACTGAGCATGCAAGG+AGG	-	chr3.3:44575190-44575209	MS.gene70955:CDS	50.0%
	CTTGCCAATCGACGAATTGC+TGG	+	chr3.3:44575075-44575094	None:intergenic	50.0%
	GAGGAAGACAGTGACTGCTA+TGG	-	chr3.3:44575209-44575228	MS.gene70955:CDS	50.0%
	GGAGAATGTGATTCGTGACG+CGG	-	chr3.3:44575164-44575183	MS.gene70955:CDS	50.0%
	GGAGGTAAGGGACTAGGAAA+GGG	-	chr3.3:44574994-44575013	MS.gene70955:CDS	50.0%
!	CAATCGACGAATTGCTGGCT+TGG	+	chr3.3:44575070-44575089	None:intergenic	50.0%
	AGGTCGTGGAAAGGGAGGTA+AGG	-	chr3.3:44574981-44575000	MS.gene70955:CDS	55.0%
	ATGTCAGGTCGTGGAAAGGG+AGG	-	chr3.3:44574976-44574995	MS.gene70955:CDS	55.0%
	GGAAAGGGAGGTAAGGGACT+AGG	-	chr3.3:44574988-44575007	MS.gene70955:CDS	55.0%
	GGGAGGTAAGGGACTAGGAA+AGG	-	chr3.3:44574993-44575012	MS.gene70955:CDS	55.0%
	GGTAAGGGACTAGGAAAGGG+AGG	-	chr3.3:44574997-44575016	MS.gene70955:CDS	55.0%
	GGTCGTGGAAAGGGAGGTAA+GGG	-	chr3.3:44574982-44575001	MS.gene70955:CDS	55.0%
!	CGTCGATTGGCAAGAAGAGG+TGG	-	chr3.3:44575081-44575100	MS.gene70955:CDS	55.0%
!!	TTGCTGGCTTGGTGATACCC+TGG	+	chr3.3:44575059-44575078	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	44574976	44575287	44574976	ID=MS.gene70955
chr3.3	mRNA	44574976	44575287	44574976	ID=MS.gene70955.t1;Parent=MS.gene70955
chr3.3	exon	44574976	44575287	44574976	ID=MS.gene70955.t1.exon1;Parent=MS.gene70955.t1
chr3.3	CDS	44574976	44575287	44574976	ID=cds.MS.gene70955.t1;Parent=MS.gene70955.t1

Gene Sequence	>MS.gene70955 ATGTCAGGTCGTGGAAAGGGAGGTAAGGGACTAGGAAAGGGAGGTGCAAAGAGACATCGAAAAGTTCTTCGTGACAACATCCAGGGTATCACCAAGCCAGCAATTCGTCGATTGGCAAGAAGAGGTGGTGTTAAGAGAATCAGCGGCTTGATCTATGAGGAAACAAGAGGTGTTCTTAAGATTTTCTTGGAGAATGTGATTCGTGACGCGGTGACATACACTGAGCATGCAAGGAGGAAGACAGTGACTGCTATGGATGTTGTTTATGCTTTGAAGAGACAAGGAAGAACGCTTTATGGATTCGGAGGTTGA
Protein sequence	>MS.gene70955.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG