AlfalfaGEDB | MS.gene018578

M. sativa cultivar XinJiangDaYe / MS.gene018578

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene018578.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene018578.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene018578.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene31196	MS.gene018578	PPI
MS.gene89488	MS.gene018578	PPI
MS.gene018578	MS.gene018580	PPI
MS.gene018578	MS.gene004124	PPI
MS.gene04068	MS.gene018578	PPI
MS.gene061867	MS.gene018578	PPI
MS.gene04434	MS.gene018578	PPI
MS.gene018578	MS.gene64650	PPI
MS.gene018578	MS.gene60093	PPI
MS.gene08121	MS.gene018578	PPI
MS.gene018578	MS.gene061874	PPI
MS.gene022932	MS.gene018578	PPI
MS.gene88681	MS.gene018578	PPI
MS.gene035718	MS.gene018578	PPI
MS.gene035896	MS.gene018578	PPI
MS.gene73136	MS.gene018578	PPI
MS.gene008276	MS.gene018578	PPI
MS.gene018578	MS.gene04972	PPI
MS.gene070907	MS.gene018578	PPI
MS.gene018578	MS.gene004121	PPI
MS.gene004124	MS.gene018578	PPI
MS.gene009027	MS.gene018578	PPI
MS.gene09873	MS.gene018578	PPI
MS.gene78578	MS.gene018578	PPI
MS.gene010219	MS.gene018578	PPI
MS.gene04052	MS.gene018578	PPI
MS.gene08122	MS.gene018578	PPI
MS.gene060465	MS.gene018578	PPI
MS.gene048705	MS.gene018578	PPI
MS.gene05623	MS.gene018578	PPI
MS.gene043967	MS.gene018578	PPI
MS.gene84536	MS.gene018578	PPI
MS.gene04972	MS.gene018578	PPI
MS.gene028629	MS.gene018578	PPI
MS.gene018578	MS.gene89488	PPI
MS.gene04067	MS.gene018578	PPI
MS.gene71215	MS.gene018578	PPI
MS.gene061866	MS.gene018578	PPI
MS.gene061870	MS.gene018578	PPI
MS.gene004121	MS.gene018578	PPI
MS.gene66225	MS.gene018578	PPI
MS.gene071136	MS.gene018578	PPI
MS.gene070242	MS.gene018578	PPI
MS.gene012390	MS.gene018578	PPI
MS.gene79195	MS.gene018578	PPI
MS.gene035721	MS.gene018578	PPI
MS.gene018578	MS.gene016624	PPI
MS.gene056215	MS.gene018578	PPI
MS.gene064557	MS.gene018578	PPI
MS.gene038469	MS.gene018578	PPI
MS.gene82546	MS.gene018578	PPI
MS.gene61017	MS.gene018578	PPI
MS.gene04433	MS.gene018578	PPI
MS.gene04452	MS.gene018578	PPI
MS.gene64650	MS.gene018578	PPI
MS.gene035787	MS.gene018578	PPI
MS.gene061874	MS.gene018578	PPI
MS.gene89487	MS.gene018578	PPI
MS.gene035719	MS.gene018578	PPI
MS.gene018578	MS.gene049705	PPI
MS.gene58854	MS.gene018578	PPI
MS.gene016624	MS.gene018578	PPI
MS.gene70955	MS.gene018578	PPI
MS.gene040383	MS.gene018578	PPI
MS.gene04392	MS.gene018578	PPI
MS.gene072493	MS.gene018578	PPI
MS.gene035720	MS.gene018578	PPI
MS.gene30920	MS.gene018578	PPI
MS.gene048746	MS.gene018578	PPI
MS.gene010220	MS.gene018578	PPI
MS.gene08053	MS.gene018578	PPI
MS.gene061872	MS.gene018578	PPI
MS.gene018578	MS.gene68545	PPI
MS.gene88680	MS.gene018578	PPI
MS.gene68545	MS.gene018578	PPI
MS.gene070241	MS.gene018578	PPI
MS.gene061869	MS.gene018578	PPI
MS.gene049821	MS.gene018578	PPI
MS.gene022946	MS.gene018578	PPI
MS.gene62520	MS.gene018578	PPI
MS.gene018580	MS.gene018578	PPI
MS.gene04064	MS.gene018578	PPI
MS.gene061876	MS.gene018578	PPI
MS.gene05619	MS.gene018578	PPI
MS.gene05608	MS.gene018578	PPI
MS.gene012391	MS.gene018578	PPI
MS.gene049705	MS.gene018578	PPI
MS.gene070244	MS.gene018578	PPI
MS.gene09871	MS.gene018578	PPI
MS.gene035897	MS.gene018578	PPI
MS.gene64920	MS.gene018578	PPI
MS.gene04576	MS.gene018578	PPI
MS.gene060463	MS.gene018578	PPI
MS.gene60093	MS.gene018578	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene018578.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene018578.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene018578.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene018578.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene018578.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene018578.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene018578.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene018578.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene018578.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene018578.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene018578.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene018578.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene018578.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene018578.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene018578.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene018578.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene018578.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene018578.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene018578.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene018578.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene018578.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene018578.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene018578.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene018578.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene018578.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene018578.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene018578.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 32 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGTGTTCTCAAGATCTTCT+TGG	0.324000	7.4:+10816173	MS.gene018578:CDS
GGAAAGGGATTGGGAAAGGG+AGG	0.412951	7.4:+10816027	MS.gene018578:CDS
TCGCAGGCTTTGTTATGCCT+TGG	0.436279	7.4:-10816086	None:intergenic
AAAATGTCTGGCCGCGGAAA+AGG	0.446222	7.4:+10816003	None:intergenic
TGTTTATGCTTTGAAGAGAC+AGG	0.447929	7.4:+10816266	MS.gene018578:CDS
GGAGGAAAGGGATTGGGAAA+GGG	0.462159	7.4:+10816024	MS.gene018578:CDS
GGAAGGACTCTCTACGGTTT+CGG	0.462635	7.4:+10816288	MS.gene018578:CDS
AGGAGGAAAGGGATTGGGAA+AGG	0.466050	7.4:+10816023	MS.gene018578:CDS
GGAAAAGGAGGAAAGGGATT+GGG	0.479340	7.4:+10816018	MS.gene018578:CDS
CGGAAAAGGAGGAAAGGGAT+TGG	0.482086	7.4:+10816017	MS.gene018578:CDS
CTGCGATTCGTCGTCTTGCA+AGG	0.502831	7.4:+10816103	MS.gene018578:CDS
GGCCGCGGAAAAGGAGGAAA+GGG	0.550993	7.4:+10816012	MS.gene018578:CDS
CTTGCAAGACGACGAATCGC+AGG	0.552192	7.4:-10816102	None:intergenic
AGGAGCAAAGAGGCACAGAA+AGG	0.564264	7.4:+10816047	MS.gene018578:CDS
TTACTTACACTGAGCACGCT+AGG	0.572434	7.4:+10816217	MS.gene018578:CDS
ATGTCTGGCCGCGGAAAAGG+AGG	0.578066	7.4:+10816006	MS.gene018578:CDS
GAGGAAGACTGTTACCGCCA+TGG	0.588179	7.4:+10816239	MS.gene018578:CDS
TGGCCGCGGAAAAGGAGGAA+AGG	0.594714	7.4:+10816011	MS.gene018578:CDS
AGACAGGGAAGGACTCTCTA+CGG	0.604561	7.4:+10816282	MS.gene018578:CDS
AGGACTCTCTACGGTTTCGG+TGG	0.607233	7.4:+10816291	MS.gene018578:CDS
CAAAGCATAAACAACATCCA+TGG	0.612707	7.4:-10816256	None:intergenic
CGTCGTCTTGCAAGGCGTGG+TGG	0.615268	7.4:+10816111	MS.gene018578:CDS
GTTTATGCTTTGAAGAGACA+GGG	0.618996	7.4:+10816267	MS.gene018578:CDS
ATGCTTTGAAGAGACAGGGA+AGG	0.619457	7.4:+10816271	MS.gene018578:CDS
ATTCGTCGTCTTGCAAGGCG+TGG	0.619919	7.4:+10816108	MS.gene018578:CDS
GTTCTTCGCGATAACATCCA+AGG	0.633409	7.4:+10816069	MS.gene018578:CDS
AGCGTGCTCAGTGTAAGTAA+CGG	0.650649	7.4:-10816214	None:intergenic
CTTACACTGAGCACGCTAGG+AGG	0.678435	7.4:+10816220	MS.gene018578:CDS
TGGGAAAGGGAGGAGCAAAG+AGG	0.685544	7.4:+10816037	MS.gene018578:CDS
GGTGGTGTGAAGAGAATCAG+TGG	0.694412	7.4:+10816129	MS.gene018578:CDS
CTCATCTATGAAGAGACTCG+CGG	0.697662	7.4:+10816153	MS.gene018578:CDS
AGCATAAACAACATCCATGG+CGG	0.725411	7.4:-10816253	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CAAAGCATAAACAACATCCA+TGG	-	chr7.4:10816259-10816278	None:intergenic	35.0%
!!	GTTTATGCTTTGAAGAGACA+GGG	+	chr7.4:10816267-10816286	MS.gene018578:CDS	35.0%
!!	TGTTTATGCTTTGAAGAGAC+AGG	+	chr7.4:10816266-10816285	MS.gene018578:CDS	35.0%
	AGCATAAACAACATCCATGG+CGG	-	chr7.4:10816256-10816275	None:intergenic	40.0%
	AGCGTGCTCAGTGTAAGTAA+CGG	-	chr7.4:10816217-10816236	None:intergenic	45.0%
	CGGTGTTCTCAAGATCTTCT+TGG	+	chr7.4:10816173-10816192	MS.gene018578:CDS	45.0%
	CTCATCTATGAAGAGACTCG+CGG	+	chr7.4:10816153-10816172	MS.gene018578:CDS	45.0%
	GGAAAAGGAGGAAAGGGATT+GGG	+	chr7.4:10816018-10816037	MS.gene018578:CDS	45.0%
	GTTCTTCGCGATAACATCCA+AGG	+	chr7.4:10816069-10816088	MS.gene018578:CDS	45.0%
	TTACTTACACTGAGCACGCT+AGG	+	chr7.4:10816217-10816236	MS.gene018578:CDS	45.0%
!!	ATGCTTTGAAGAGACAGGGA+AGG	+	chr7.4:10816271-10816290	MS.gene018578:CDS	45.0%
	AGACAGGGAAGGACTCTCTA+CGG	+	chr7.4:10816282-10816301	MS.gene018578:CDS	50.0%
	AGGAGGAAAGGGATTGGGAA+AGG	+	chr7.4:10816023-10816042	MS.gene018578:CDS	50.0%
	CGGAAAAGGAGGAAAGGGAT+TGG	+	chr7.4:10816017-10816036	MS.gene018578:CDS	50.0%
	GGAAGGACTCTCTACGGTTT+CGG	+	chr7.4:10816288-10816307	MS.gene018578:CDS	50.0%
	GGAGGAAAGGGATTGGGAAA+GGG	+	chr7.4:10816024-10816043	MS.gene018578:CDS	50.0%
	GGTGGTGTGAAGAGAATCAG+TGG	+	chr7.4:10816129-10816148	MS.gene018578:CDS	50.0%
!	AGGAGCAAAGAGGCACAGAA+AGG	+	chr7.4:10816047-10816066	MS.gene018578:CDS	50.0%
!	ATCCCTTTCCTCCTTTTCCG+CGG	-	chr7.4:10816017-10816036	None:intergenic	50.0%
!!	TCGCAGGCTTTGTTATGCCT+TGG	-	chr7.4:10816089-10816108	None:intergenic	50.0%
	AGGACTCTCTACGGTTTCGG+TGG	+	chr7.4:10816291-10816310	MS.gene018578:CDS	55.0%
	ATTCGTCGTCTTGCAAGGCG+TGG	+	chr7.4:10816108-10816127	MS.gene018578:CDS	55.0%
	CTGCGATTCGTCGTCTTGCA+AGG	+	chr7.4:10816103-10816122	MS.gene018578:CDS	55.0%
	CTTACACTGAGCACGCTAGG+AGG	+	chr7.4:10816220-10816239	MS.gene018578:CDS	55.0%
	CTTGCAAGACGACGAATCGC+AGG	-	chr7.4:10816105-10816124	None:intergenic	55.0%
	GAGGAAGACTGTTACCGCCA+TGG	+	chr7.4:10816239-10816258	MS.gene018578:CDS	55.0%
	GGAAAGGGATTGGGAAAGGG+AGG	+	chr7.4:10816027-10816046	MS.gene018578:CDS	55.0%
	TGGGAAAGGGAGGAGCAAAG+AGG	+	chr7.4:10816037-10816056	MS.gene018578:CDS	55.0%
	ATGTCTGGCCGCGGAAAAGG+AGG	+	chr7.4:10816006-10816025	MS.gene018578:CDS	60.0%
	GGCCGCGGAAAAGGAGGAAA+GGG	+	chr7.4:10816012-10816031	MS.gene018578:CDS	60.0%
	TGGCCGCGGAAAAGGAGGAA+AGG	+	chr7.4:10816011-10816030	MS.gene018578:CDS	60.0%
	CGTCGTCTTGCAAGGCGTGG+TGG	+	chr7.4:10816111-10816130	MS.gene018578:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	10816006	10816317	10816006	ID=MS.gene018578
chr7.4	mRNA	10816006	10816317	10816006	ID=MS.gene018578.t1;Parent=MS.gene018578
chr7.4	exon	10816006	10816317	10816006	ID=MS.gene018578.t1.exon1;Parent=MS.gene018578.t1
chr7.4	CDS	10816006	10816317	10816006	ID=cds.MS.gene018578.t1;Parent=MS.gene018578.t1

Gene Sequence	>MS.gene018578 ATGTCTGGCCGCGGAAAAGGAGGAAAGGGATTGGGAAAGGGAGGAGCAAAGAGGCACAGAAAGGTTCTTCGCGATAACATCCAAGGCATAACAAAGCCTGCGATTCGTCGTCTTGCAAGGCGTGGTGGTGTGAAGAGAATCAGTGGTCTCATCTATGAAGAGACTCGCGGTGTTCTCAAGATCTTCTTGGAGAACGTGATTCGTGATGCCGTTACTTACACTGAGCACGCTAGGAGGAAGACTGTTACCGCCATGGATGTTGTTTATGCTTTGAAGAGACAGGGAAGGACTCTCTACGGTTTCGGTGGTTAA
Protein sequence	>MS.gene018578.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG