AlfalfaGEDB | MS.gene04434

M. sativa cultivar XinJiangDaYe / MS.gene04434

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04434.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04434.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04434.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene040383	MS.gene04434	PPI
MS.gene04434	MS.gene60093	PPI
MS.gene070242	MS.gene04434	PPI
MS.gene04434	MS.gene061869	PPI
MS.gene79195	MS.gene04434	PPI
MS.gene035721	MS.gene04434	PPI
MS.gene056215	MS.gene04434	PPI
MS.gene04434	MS.gene04972	PPI
MS.gene064557	MS.gene04434	PPI
MS.gene04434	MS.gene004121	PPI
MS.gene038469	MS.gene04434	PPI
MS.gene04434	MS.gene018578	PPI
MS.gene04434	MS.gene061867	PPI
MS.gene04452	MS.gene04434	PPI
MS.gene04434	MS.gene04064	PPI
MS.gene04434	MS.gene018580	PPI
MS.gene035787	MS.gene04434	PPI
MS.gene09871	MS.gene04434	PPI
MS.gene04434	MS.gene05619	PPI
MS.gene89487	MS.gene04434	PPI
MS.gene04434	MS.gene04068	PPI
MS.gene04434	MS.gene04052	PPI
MS.gene04434	MS.gene89488	PPI
MS.gene035719	MS.gene04434	PPI
MS.gene58854	MS.gene04434	PPI
MS.gene04434	MS.gene79195	PPI
MS.gene04434	MS.gene048705	PPI
MS.gene04434	MS.gene61017	PPI
MS.gene04434	MS.gene061874	PPI
MS.gene04392	MS.gene04434	PPI
MS.gene04434	MS.gene04576	PPI
MS.gene072493	MS.gene04434	PPI
MS.gene30920	MS.gene04434	PPI
MS.gene048746	MS.gene04434	PPI
MS.gene010220	MS.gene04434	PPI
MS.gene08053	MS.gene04434	PPI
MS.gene061872	MS.gene04434	PPI
MS.gene04434	MS.gene012390	PPI
MS.gene88680	MS.gene04434	PPI
MS.gene070241	MS.gene04434	PPI
MS.gene022946	MS.gene04434	PPI
MS.gene061876	MS.gene04434	PPI
MS.gene04434	MS.gene016624	PPI
MS.gene04434	MS.gene008276	PPI
MS.gene012391	MS.gene04434	PPI
MS.gene88681	MS.gene04434	PPI
MS.gene070244	MS.gene04434	PPI
MS.gene035897	MS.gene04434	PPI
MS.gene04434	MS.gene73136	PPI
MS.gene64920	MS.gene04434	PPI
MS.gene04434	MS.gene049821	PPI
MS.gene060463	MS.gene04434	PPI
MS.gene04434	MS.gene05623	PPI
MS.gene31196	MS.gene04434	PPI
MS.gene04434	MS.gene035720	PPI
MS.gene060465	MS.gene04434	PPI
MS.gene04434	MS.gene09871	PPI
MS.gene04068	MS.gene04434	PPI
MS.gene04434	MS.gene049705	PPI
MS.gene04434	MS.gene070907	PPI
MS.gene04434	MS.gene04392	PPI
MS.gene04434	MS.gene010219	PPI
MS.gene04434	MS.gene05608	PPI
MS.gene08121	MS.gene04434	PPI
MS.gene04434	MS.gene68545	PPI
MS.gene022932	MS.gene04434	PPI
MS.gene035718	MS.gene04434	PPI
MS.gene04434	MS.gene82546	PPI
MS.gene035896	MS.gene04434	PPI
MS.gene04434	MS.gene048746	PPI
MS.gene04434	MS.gene08122	PPI
MS.gene009027	MS.gene04434	PPI
MS.gene04434	MS.gene09873	PPI
MS.gene78578	MS.gene04434	PPI
MS.gene05623	MS.gene04434	PPI
MS.gene043967	MS.gene04434	PPI
MS.gene04434	MS.gene62520	PPI
MS.gene04434	MS.gene035897	PPI
MS.gene028629	MS.gene04434	PPI
MS.gene04433	MS.gene04434	PPI
MS.gene04434	MS.gene004124	PPI
MS.gene04067	MS.gene04434	PPI
MS.gene71215	MS.gene04434	PPI
MS.gene061870	MS.gene04434	PPI
MS.gene04434	MS.gene84536	PPI
MS.gene04434	MS.gene061866	PPI
MS.gene04434	MS.gene64650	PPI
MS.gene66225	MS.gene04434	PPI
MS.gene70955	MS.gene04434	PPI
MS.gene071136	MS.gene04434	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04434.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04434.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04434.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04434.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04434.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04434.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04434.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04434.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04434.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04434.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04434.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04434.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04434.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04434.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04434.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene04434.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04434.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04434.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04434.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04434.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04434.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04434.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04434.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04434.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04434.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04434.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04434.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 37 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTTCTTCCTTGTCTCTTCA+AGG	0.367893	3.4:+57893836	None:intergenic
CGTCTTCCTCCTTGCGTGCT+CGG	0.372219	3.4:+57893884	None:intergenic
AGACAAGGAAGAACCCTCTA+TGG	0.396310	3.4:-57893828	MS.gene04434:CDS
AGGTGTGTTGAAGATCTTCT+TGG	0.432619	3.4:-57893937	MS.gene04434:CDS
CTTGCCAATCGACGAATGGC+AGG	0.473855	3.4:+57894008	None:intergenic
TTCAACCTCCGAATCCATAG+AGG	0.479629	3.4:+57893814	None:intergenic
CTGTTGAAAATGTCAGGTCG+TGG	0.487154	3.4:-57894113	None:intergenic
GGTAAGGGACTCGGAAAGGG+AGG	0.488695	3.4:-57894083	MS.gene04434:CDS
GGAAGAACCCTCTATGGATT+CGG	0.491141	3.4:-57893822	MS.gene04434:CDS
AGGTCGTGGAAAGGGAGGTA+AGG	0.492819	3.4:-57894099	MS.gene04434:CDS
GGAGGTAAGGGACTCGGAAA+GGG	0.499726	3.4:-57894086	MS.gene04434:CDS
AAAGCCTGCCATTCGTCGAT+TGG	0.502268	3.4:-57894012	MS.gene04434:CDS
GAAGATCTTCAACACACCTC+TGG	0.516600	3.4:+57893941	None:intergenic
GGGAGGTAAGGGACTCGGAA+AGG	0.518226	3.4:-57894087	MS.gene04434:CDS
AGTTCTTCGTGATAACATCC+AGG	0.522451	3.4:-57894042	MS.gene04434:CDS
GGAAAGGGAGGTAAGGGACT+CGG	0.535187	3.4:-57894092	MS.gene04434:CDS
AAAATGTCAGGTCGTGGAAA+GGG	0.548959	3.4:-57894107	MS.gene04434:CDS
GAGGAAGACGGTGACAGCCA+TGG	0.561434	3.4:-57893871	MS.gene04434:CDS
GGTGGTGTTAAGAGAATCAG+TGG	0.562326	3.4:-57893981	MS.gene04434:CDS
GGTCGTGGAAAGGGAGGTAA+GGG	0.564245	3.4:-57894098	MS.gene04434:CDS
TGACATACACCGAGCACGCA+AGG	0.566909	3.4:-57893893	MS.gene04434:CDS
AGGGAGGTGCAAAGAGACAT+AGG	0.568952	3.4:-57894067	MS.gene04434:CDS
GAAAATGTCAGGTCGTGGAA+AGG	0.584382	3.4:-57894108	None:intergenic
TCTTCTTGCCAATCGACGAA+TGG	0.586064	3.4:+57894004	None:intergenic
CGTCGATTGGCAAGAAGAGG+TGG	0.589209	3.4:-57893999	MS.gene04434:CDS
GTCTATGCCTTGAAGAGACA+AGG	0.608946	3.4:-57893843	MS.gene04434:CDS
TGGCAGGCTTTGTGATACCC+TGG	0.617511	3.4:+57894024	None:intergenic
CGAGCACGCAAGGAGGAAGA+CGG	0.619274	3.4:-57893883	MS.gene04434:CDS
ATTCGTCGATTGGCAAGAAG+AGG	0.624691	3.4:-57894002	MS.gene04434:CDS
CAAGGCATAGACAACATCCA+TGG	0.629842	3.4:+57893854	None:intergenic
CGGTGTATGTCACAGCATCA+CGG	0.631400	3.4:+57893904	None:intergenic
GTTCTTCGTGATAACATCCA+GGG	0.641996	3.4:-57894041	MS.gene04434:CDS
ATGTCAGGTCGTGGAAAGGG+AGG	0.643397	3.4:-57894104	MS.gene04434:CDS
TCAACCTCCGAATCCATAGA+GGG	0.644865	3.4:+57893815	None:intergenic
AGAACCCTCTATGGATTCGG+AGG	0.656308	3.4:-57893819	MS.gene04434:CDS
TTGATATATGAAGAAACCAG+AGG	0.683025	3.4:-57893957	MS.gene04434:CDS
CATACACCGAGCACGCAAGG+AGG	0.730442	3.4:-57893890	MS.gene04434:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGATATATGAAGAAACCAG+AGG	-	chr3.4:57893962-57893981	MS.gene04434:CDS	30.0%
	AGGTGTGTTGAAGATCTTCT+TGG	-	chr3.4:57893982-57894001	MS.gene04434:CDS	40.0%
	AGTTCTTCGTGATAACATCC+AGG	-	chr3.4:57893877-57893896	MS.gene04434:CDS	40.0%
	GTTCTTCGTGATAACATCCA+GGG	-	chr3.4:57893878-57893897	MS.gene04434:CDS	40.0%
	AGACAAGGAAGAACCCTCTA+TGG	-	chr3.4:57894091-57894110	MS.gene04434:CDS	45.0%
	CAAGGCATAGACAACATCCA+TGG	+	chr3.4:57894068-57894087	None:intergenic	45.0%
	GAAGATCTTCAACACACCTC+TGG	+	chr3.4:57893981-57894000	None:intergenic	45.0%
	GGAAGAACCCTCTATGGATT+CGG	-	chr3.4:57894097-57894116	MS.gene04434:CDS	45.0%
	GGAAGAACCCTCTATGGATT+TGG	-	chr3.4:57894097-57894116	MS.gene04434:CDS	45.0%
	GGTTCTTCCTTGTCTCTTCA+AGG	+	chr3.4:57894086-57894105	None:intergenic	45.0%
	GTCTATGCCTTGAAGAGACA+AGG	-	chr3.4:57894076-57894095	MS.gene04434:CDS	45.0%
	TCAACCTCCGAATCCATAGA+GGG	+	chr3.4:57894107-57894126	None:intergenic	45.0%
	TCTTCTTGCCAATCGACGAA+TGG	+	chr3.4:57893918-57893937	None:intergenic	45.0%
!	ATTCGTCGATTGGCAAGAAG+AGG	-	chr3.4:57893917-57893936	MS.gene04434:CDS	45.0%
!	GGTGGTGTTAAGAGAATCAG+TGG	-	chr3.4:57893938-57893957	MS.gene04434:CDS	45.0%
	AAAGCCTGCCATTCGTCGAT+TGG	-	chr3.4:57893907-57893926	MS.gene04434:CDS	50.0%
	AGAACCCTCTATGGATTCGG+AGG	-	chr3.4:57894100-57894119	MS.gene04434:CDS	50.0%
	CGGTGTATGTCACAGCATCA+CGG	+	chr3.4:57894018-57894037	None:intergenic	50.0%
!	AGGGAGGTGCAAAGAGACAT+AGG	-	chr3.4:57893852-57893871	MS.gene04434:CDS	50.0%
	AGGTCGTGGAAAGGGAGGTA+AGG	-	chr3.4:57893820-57893839	MS.gene04434:CDS	55.0%
	ATGTCAGGTCGTGGAAAGGG+AGG	-	chr3.4:57893815-57893834	MS.gene04434:CDS	55.0%
	CTTGCCAATCGACGAATGGC+AGG	+	chr3.4:57893914-57893933	None:intergenic	55.0%
	GGAAAGGGAGGTAAGGGACT+CGG	-	chr3.4:57893827-57893846	MS.gene04434:CDS	55.0%
	GGAAAGGGAGGTAAGGGACT+TGG	-	chr3.4:57893827-57893846	MS.gene04434:CDS	55.0%
	GGAGGTAAGGGACTCGGAAA+GGG	-	chr3.4:57893833-57893852	MS.gene04434:CDS	55.0%
	GGTCGTGGAAAGGGAGGTAA+GGG	-	chr3.4:57893821-57893840	MS.gene04434:CDS	55.0%
	TGACATACACCGAGCACGCA+AGG	-	chr3.4:57894026-57894045	MS.gene04434:CDS	55.0%
!	CGTCGATTGGCAAGAAGAGG+TGG	-	chr3.4:57893920-57893939	MS.gene04434:CDS	55.0%
!	TGGCAGGCTTTGTGATACCC+TGG	+	chr3.4:57893898-57893917	None:intergenic	55.0%
	CATACACCGAGCACGCAAGG+AGG	-	chr3.4:57894029-57894048	MS.gene04434:CDS	60.0%
	CGAGCACGCAAGGAGGAAGA+CGG	-	chr3.4:57894036-57894055	MS.gene04434:CDS	60.0%
	CGTCTTCCTCCTTGCGTGCT+CGG	+	chr3.4:57894038-57894057	None:intergenic	60.0%
	GAGGAAGACGGTGACAGCCA+TGG	-	chr3.4:57894048-57894067	MS.gene04434:CDS	60.0%
	GGGAGGTAAGGGACTCGGAA+AGG	-	chr3.4:57893832-57893851	MS.gene04434:CDS	60.0%
	GGTAAGGGACTCGGAAAGGG+AGG	-	chr3.4:57893836-57893855	MS.gene04434:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	57893815	57894126	57893815	ID=MS.gene04434
chr3.4	mRNA	57893815	57894126	57893815	ID=MS.gene04434.t1;Parent=MS.gene04434
chr3.4	exon	57893815	57894126	57893815	ID=MS.gene04434.t1.exon1;Parent=MS.gene04434.t1
chr3.4	CDS	57893815	57894126	57893815	ID=cds.MS.gene04434.t1;Parent=MS.gene04434.t1

Gene Sequence	>MS.gene04434 ATGTCAGGTCGTGGAAAGGGAGGTAAGGGACTCGGAAAGGGAGGTGCAAAGAGACATAGGAAAGTTCTTCGTGATAACATCCAGGGTATCACAAAGCCTGCCATTCGTCGATTGGCAAGAAGAGGTGGTGTTAAGAGAATCAGTGGTTTGATATATGAAGAAACCAGAGGTGTGTTGAAGATCTTCTTGGAAAATGTCATCCGTGATGCTGTGACATACACCGAGCACGCAAGGAGGAAGACGGTGACAGCCATGGATGTTGTCTATGCCTTGAAGAGACAAGGAAGAACCCTCTATGGATTCGGAGGTTGA
Protein sequence	>MS.gene04434.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG