AlfalfaGEDB | MS.gene04392

M. sativa cultivar XinJiangDaYe / MS.gene04392

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04392.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04392.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04392.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene66225	MS.gene04392	PPI
MS.gene071136	MS.gene04392	PPI
MS.gene04392	MS.gene04972	PPI
MS.gene070242	MS.gene04392	PPI
MS.gene04392	MS.gene004121	PPI
MS.gene79195	MS.gene04392	PPI
MS.gene035721	MS.gene04392	PPI
MS.gene04392	MS.gene061867	PPI
MS.gene04392	MS.gene04064	PPI
MS.gene04392	MS.gene008276	PPI
MS.gene056215	MS.gene04392	PPI
MS.gene064557	MS.gene04392	PPI
MS.gene038469	MS.gene04392	PPI
MS.gene04392	MS.gene05619	PPI
MS.gene04392	MS.gene04068	PPI
MS.gene04433	MS.gene04392	PPI
MS.gene04452	MS.gene04392	PPI
MS.gene04392	MS.gene89488	PPI
MS.gene035787	MS.gene04392	PPI
MS.gene04392	MS.gene79195	PPI
MS.gene04392	MS.gene61017	PPI
MS.gene04392	MS.gene04576	PPI
MS.gene89487	MS.gene04392	PPI
MS.gene04392	MS.gene09871	PPI
MS.gene04392	MS.gene012390	PPI
MS.gene035719	MS.gene04392	PPI
MS.gene58854	MS.gene04392	PPI
MS.gene70955	MS.gene04392	PPI
MS.gene04392	MS.gene04434	PPI
MS.gene040383	MS.gene04392	PPI
MS.gene072493	MS.gene04392	PPI
MS.gene30920	MS.gene04392	PPI
MS.gene048746	MS.gene04392	PPI
MS.gene010220	MS.gene04392	PPI
MS.gene08053	MS.gene04392	PPI
MS.gene061872	MS.gene04392	PPI
MS.gene04392	MS.gene010219	PPI
MS.gene88680	MS.gene04392	PPI
MS.gene070241	MS.gene04392	PPI
MS.gene04392	MS.gene016624	PPI
MS.gene022946	MS.gene04392	PPI
MS.gene04392	MS.gene73136	PPI
MS.gene04392	MS.gene049821	PPI
MS.gene061876	MS.gene04392	PPI
MS.gene04392	MS.gene05623	PPI
MS.gene012391	MS.gene04392	PPI
MS.gene070244	MS.gene04392	PPI
MS.gene09871	MS.gene04392	PPI
MS.gene035897	MS.gene04392	PPI
MS.gene64920	MS.gene04392	PPI
MS.gene04392	MS.gene035720	PPI
MS.gene060463	MS.gene04392	PPI
MS.gene31196	MS.gene04392	PPI
MS.gene04392	MS.gene035897	PPI
MS.gene04392	MS.gene049705	PPI
MS.gene04392	MS.gene070907	PPI
MS.gene04392	MS.gene05608	PPI
MS.gene04068	MS.gene04392	PPI
MS.gene04392	MS.gene68545	PPI
MS.gene04434	MS.gene04392	PPI
MS.gene04392	MS.gene82546	PPI
MS.gene08121	MS.gene04392	PPI
MS.gene04392	MS.gene048746	PPI
MS.gene04392	MS.gene60093	PPI
MS.gene04392	MS.gene08122	PPI
MS.gene022932	MS.gene04392	PPI
MS.gene04392	MS.gene09873	PPI
MS.gene88681	MS.gene04392	PPI
MS.gene035718	MS.gene04392	PPI
MS.gene035896	MS.gene04392	PPI
MS.gene009027	MS.gene04392	PPI
MS.gene04392	MS.gene62520	PPI
MS.gene04392	MS.gene018578	PPI
MS.gene78578	MS.gene04392	PPI
MS.gene060465	MS.gene04392	PPI
MS.gene04392	MS.gene018580	PPI
MS.gene04392	MS.gene004124	PPI
MS.gene05623	MS.gene04392	PPI
MS.gene043967	MS.gene04392	PPI
MS.gene028629	MS.gene04392	PPI
MS.gene04392	MS.gene04052	PPI
MS.gene04392	MS.gene84536	PPI
MS.gene04392	MS.gene061866	PPI
MS.gene04067	MS.gene04392	PPI
MS.gene71215	MS.gene04392	PPI
MS.gene04392	MS.gene64650	PPI
MS.gene061870	MS.gene04392	PPI
MS.gene04392	MS.gene048705	PPI
MS.gene04392	MS.gene061874	PPI
MS.gene04392	MS.gene061869	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04392.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04392.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04392.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04392.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04392.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04392.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04392.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04392.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04392.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04392.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04392.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04392.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04392.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04392.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04392.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene04392.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04392.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04392.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04392.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04392.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04392.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04392.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04392.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04392.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04392.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04392.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04392.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 34 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAAGAACCCTCTATGGATT+TGG	0.370047	3.4:-57281291	MS.gene04392:CDS
CAATCGACGAATGGCAGGTT+TGG	0.388444	3.4:+57281482	None:intergenic
AGACAAGGAAGAACCCTCTA+TGG	0.396310	3.4:-57281297	MS.gene04392:CDS
AGGTGTCTTGAAGATCTTCT+TGG	0.396647	3.4:-57281406	MS.gene04392:CDS
GGAAAGGGAGGTAAGGGATT+GGG	0.427677	3.4:-57281561	MS.gene04392:CDS
CTCTTGAAAATGTCAGGTCG+TGG	0.456977	3.4:-57281582	None:intergenic
GGAGGTAAGGGATTGGGAAA+GGG	0.467307	3.4:-57281555	MS.gene04392:CDS
CTTGCCAATCGACGAATGGC+AGG	0.470081	3.4:+57281477	None:intergenic
GGGAGGTAAGGGATTGGGAA+AGG	0.474781	3.4:-57281556	MS.gene04392:CDS
TTCAACCTCCAAATCCATAG+AGG	0.476286	3.4:+57281283	None:intergenic
GGTAAGGGATTGGGAAAGGG+AGG	0.489050	3.4:-57281552	MS.gene04392:CDS
AGGTCGTGGAAAGGGAGGTA+AGG	0.492819	3.4:-57281568	MS.gene04392:CDS
CAAACCTGCCATTCGTCGAT+TGG	0.506455	3.4:-57281481	MS.gene04392:CDS
AGAACCCTCTATGGATTTGG+AGG	0.508817	3.4:-57281288	MS.gene04392:CDS
TGGAAAGGGAGGTAAGGGAT+TGG	0.528271	3.4:-57281562	MS.gene04392:CDS
AAAATGTCAGGTCGTGGAAA+GGG	0.538992	3.4:-57281576	MS.gene04392:CDS
AGGGAGGTGCAAAGAGGCAT+AGG	0.547891	3.4:-57281536	MS.gene04392:CDS
GGTCGTGGAAAGGGAGGTAA+GGG	0.574068	3.4:-57281567	MS.gene04392:CDS
TGGCAGGTTTGGTGATACCT+TGG	0.580063	3.4:+57281493	None:intergenic
GAAAATGTCAGGTCGTGGAA+AGG	0.584382	3.4:-57281577	None:intergenic
TCTTCTTGCCAATCGACGAA+TGG	0.586064	3.4:+57281473	None:intergenic
CGTCGATTGGCAAGAAGAGG+TGG	0.589209	3.4:-57281468	MS.gene04392:CDS
TTACATACACTGAGCACGCA+AGG	0.596203	3.4:-57281362	MS.gene04392:CDS
GTCTATGCATTGAAGAGACA+AGG	0.605762	3.4:-57281312	MS.gene04392:CDS
GGTGGTGTCAAGAGAATCAG+TGG	0.607763	3.4:-57281450	MS.gene04392:CDS
GAGGAAGACAGTGACAGCCA+TGG	0.615260	3.4:-57281340	MS.gene04392:CDS
GTTCTTCGTGACAACATCCA+AGG	0.620188	3.4:-57281510	MS.gene04392:CDS
CAATGCATAGACAACATCCA+TGG	0.623752	3.4:+57281323	None:intergenic
ATTCGTCGATTGGCAAGAAG+AGG	0.624691	3.4:-57281471	MS.gene04392:CDS
TTGATCTATGAAGAAACAAG+AGG	0.629001	3.4:-57281426	MS.gene04392:CDS
TCAACCTCCAAATCCATAGA+GGG	0.633476	3.4:+57281284	None:intergenic
ATGTCAGGTCGTGGAAAGGG+AGG	0.643397	3.4:-57281573	MS.gene04392:CDS
TGGGAAAGGGAGGTGCAAAG+AGG	0.673404	3.4:-57281542	MS.gene04392:CDS
CATACACTGAGCACGCAAGG+AGG	0.730442	3.4:-57281359	MS.gene04392:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGATCTATGAAGAAACAAG+AGG	-	chr3.4:57281431-57281450	MS.gene04392:CDS	30.0%
	AGGTGTCTTGAAGATCTTCT+TGG	-	chr3.4:57281451-57281470	MS.gene04392:CDS	40.0%
	CAATGCATAGACAACATCCA+TGG	+	chr3.4:57281537-57281556	None:intergenic	40.0%
	GTCTATGCATTGAAGAGACA+AGG	-	chr3.4:57281545-57281564	MS.gene04392:CDS	40.0%
	TCAACCTCCAAATCCATAGA+GGG	+	chr3.4:57281576-57281595	None:intergenic	40.0%
	AGAACCCTCTATGGATTTGG+AGG	-	chr3.4:57281569-57281588	MS.gene04392:CDS	45.0%
	AGACAAGGAAGAACCCTCTA+TGG	-	chr3.4:57281560-57281579	MS.gene04392:CDS	45.0%
	GGAAGAACCCTCTATGGATT+TGG	-	chr3.4:57281566-57281585	MS.gene04392:CDS	45.0%
	GTTCTTCGTGACAACATCCA+AGG	-	chr3.4:57281347-57281366	MS.gene04392:CDS	45.0%
	TCTTCTTGCCAATCGACGAA+TGG	+	chr3.4:57281387-57281406	None:intergenic	45.0%
	TTACATACACTGAGCACGCA+AGG	-	chr3.4:57281495-57281514	MS.gene04392:CDS	45.0%
!	ATTCGTCGATTGGCAAGAAG+AGG	-	chr3.4:57281386-57281405	MS.gene04392:CDS	45.0%
	CAAACCTGCCATTCGTCGAT+TGG	-	chr3.4:57281376-57281395	MS.gene04392:CDS	50.0%
	CAATCGACGAATGGCAGGTT+TGG	+	chr3.4:57281378-57281397	None:intergenic	50.0%
	GGAAAGGGAGGTAAGGGATT+GGG	-	chr3.4:57281296-57281315	MS.gene04392:CDS	50.0%
	GGAGGTAAGGGATTGGGAAA+GGG	-	chr3.4:57281302-57281321	MS.gene04392:CDS	50.0%
	TGGAAAGGGAGGTAAGGGAT+TGG	-	chr3.4:57281295-57281314	MS.gene04392:CDS	50.0%
!	GGTGGTGTCAAGAGAATCAG+TGG	-	chr3.4:57281407-57281426	MS.gene04392:CDS	50.0%
!!	TGGCAGGTTTGGTGATACCT+TGG	+	chr3.4:57281367-57281386	None:intergenic	50.0%
	AGGTCGTGGAAAGGGAGGTA+AGG	-	chr3.4:57281289-57281308	MS.gene04392:CDS	55.0%
	ATGTCAGGTCGTGGAAAGGG+AGG	-	chr3.4:57281284-57281303	MS.gene04392:CDS	55.0%
	CATACACTGAGCACGCAAGG+AGG	-	chr3.4:57281498-57281517	MS.gene04392:CDS	55.0%
	CTTGCCAATCGACGAATGGC+AGG	+	chr3.4:57281383-57281402	None:intergenic	55.0%
	GAGGAAGACAGTGACAGCCA+TGG	-	chr3.4:57281517-57281536	MS.gene04392:CDS	55.0%
	GGGAGGTAAGGGATTGGGAA+AGG	-	chr3.4:57281301-57281320	MS.gene04392:CDS	55.0%
	GGTAAGGGATTGGGAAAGGG+AGG	-	chr3.4:57281305-57281324	MS.gene04392:CDS	55.0%
	GGTCGTGGAAAGGGAGGTAA+GGG	-	chr3.4:57281290-57281309	MS.gene04392:CDS	55.0%
!	AGGGAGGTGCAAAGAGGCAT+AGG	-	chr3.4:57281321-57281340	MS.gene04392:CDS	55.0%
!	CGTCGATTGGCAAGAAGAGG+TGG	-	chr3.4:57281389-57281408	MS.gene04392:CDS	55.0%
!	TGGGAAAGGGAGGTGCAAAG+AGG	-	chr3.4:57281315-57281334	MS.gene04392:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	57281284	57281595	57281284	ID=MS.gene04392
chr3.4	mRNA	57281284	57281595	57281284	ID=MS.gene04392.t1;Parent=MS.gene04392
chr3.4	exon	57281284	57281595	57281284	ID=MS.gene04392.t1.exon1;Parent=MS.gene04392.t1
chr3.4	CDS	57281284	57281595	57281284	ID=cds.MS.gene04392.t1;Parent=MS.gene04392.t1

Gene Sequence	>MS.gene04392 ATGTCAGGTCGTGGAAAGGGAGGTAAGGGATTGGGAAAGGGAGGTGCAAAGAGGCATAGGAAAGTTCTTCGTGACAACATCCAAGGTATCACCAAACCTGCCATTCGTCGATTGGCAAGAAGAGGTGGTGTCAAGAGAATCAGTGGATTGATCTATGAAGAAACAAGAGGTGTCTTGAAGATCTTCTTGGAAAACGTCATTCGTGATGCAGTTACATACACTGAGCACGCAAGGAGGAAGACAGTGACAGCCATGGATGTTGTCTATGCATTGAAGAGACAAGGAAGAACCCTCTATGGATTTGGAGGTTGA
Protein sequence	>MS.gene04392.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG