AlfalfaGEDB | MS.gene04433

M. sativa cultivar XinJiangDaYe / MS.gene04433

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04433.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04433.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04433.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene58854	MS.gene04433	PPI
MS.gene04433	MS.gene022946	PPI
MS.gene04433	MS.gene05623	PPI
MS.gene071136	MS.gene04433	PPI
MS.gene04433	MS.gene58854	PPI
MS.gene04433	MS.gene035720	PPI
MS.gene04433	MS.gene70955	PPI
MS.gene04433	MS.gene09871	PPI
MS.gene88680	MS.gene04433	PPI
MS.gene056215	MS.gene04433	PPI
MS.gene04433	MS.gene049705	PPI
MS.gene04433	MS.gene070907	PPI
MS.gene04433	MS.gene04392	PPI
MS.gene04433	MS.gene010219	PPI
MS.gene04433	MS.gene05608	PPI
MS.gene04433	MS.gene040383	PPI
MS.gene04433	MS.gene68545	PPI
MS.gene035787	MS.gene04433	PPI
MS.gene04433	MS.gene070242	PPI
MS.gene04433	MS.gene82546	PPI
MS.gene89487	MS.gene04433	PPI
MS.gene04433	MS.gene048746	PPI
MS.gene04433	MS.gene08122	PPI
MS.gene04433	MS.gene09873	PPI
MS.gene04433	MS.gene022932	PPI
MS.gene30920	MS.gene04433	PPI
MS.gene061872	MS.gene04433	PPI
MS.gene04433	MS.gene043967	PPI
MS.gene04433	MS.gene62520	PPI
MS.gene04433	MS.gene08053	PPI
MS.gene070241	MS.gene04433	PPI
MS.gene04433	MS.gene035897	PPI
MS.gene04433	MS.gene035721	PPI
MS.gene04433	MS.gene004124	PPI
MS.gene061876	MS.gene04433	PPI
MS.gene012391	MS.gene04433	PPI
MS.gene88681	MS.gene04433	PPI
MS.gene04433	MS.gene072493	PPI
MS.gene070244	MS.gene04433	PPI
MS.gene04433	MS.gene04052	PPI
MS.gene04433	MS.gene84536	PPI
MS.gene04433	MS.gene035896	PPI
MS.gene04433	MS.gene88681	PPI
MS.gene04433	MS.gene061866	PPI
MS.gene060463	MS.gene04433	PPI
MS.gene04433	MS.gene64650	PPI
MS.gene04433	MS.gene60093	PPI
MS.gene04433	MS.gene061874	PPI
MS.gene31196	MS.gene04433	PPI
MS.gene04433	MS.gene061869	PPI
MS.gene060465	MS.gene04433	PPI
MS.gene04433	MS.gene010220	PPI
MS.gene04433	MS.gene04972	PPI
MS.gene04433	MS.gene004121	PPI
MS.gene04433	MS.gene018578	PPI
MS.gene04433	MS.gene061867	PPI
MS.gene04433	MS.gene04064	PPI
MS.gene04433	MS.gene018580	PPI
MS.gene04433	MS.gene008276	PPI
MS.gene04433	MS.gene05619	PPI
MS.gene035718	MS.gene04433	PPI
MS.gene04433	MS.gene04068	PPI
MS.gene04433	MS.gene035719	PPI
MS.gene04433	MS.gene89488	PPI
MS.gene009027	MS.gene04433	PPI
MS.gene04433	MS.gene66225	PPI
MS.gene04433	MS.gene79195	PPI
MS.gene04433	MS.gene048705	PPI
MS.gene04433	MS.gene61017	PPI
MS.gene04433	MS.gene04576	PPI
MS.gene78578	MS.gene04433	PPI
MS.gene04433	MS.gene012390	PPI
MS.gene04433	MS.gene08121	PPI
MS.gene04433	MS.gene64920	PPI
MS.gene04433	MS.gene064557	PPI
MS.gene04433	MS.gene04434	PPI
MS.gene04433	MS.gene04452	PPI
MS.gene04433	MS.gene89487	PPI
MS.gene028629	MS.gene04433	PPI
MS.gene04433	MS.gene038469	PPI
MS.gene04067	MS.gene04433	PPI
MS.gene71215	MS.gene04433	PPI
MS.gene04433	MS.gene016624	PPI
MS.gene061870	MS.gene04433	PPI
MS.gene04433	MS.gene73136	PPI
MS.gene04433	MS.gene049821	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04433.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04433.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04433.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04433.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04433.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04433.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04433.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04433.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04433.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04433.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04433.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04433.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04433.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04433.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04433.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene04433.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04433.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04433.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04433.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04433.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04433.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04433.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04433.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04433.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04433.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04433.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04433.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 38 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGTGTATTGAAGATATTCT+TGG	0.398568	3.4:-57889764	MS.gene04433:CDS
AGACAAGGAAGAACCCTCTA+TGG	0.431401	3.4:-57889655	MS.gene04433:CDS
GGAAGAACCCTCTATGGTTT+CGG	0.438131	3.4:-57889649	MS.gene04433:CDS
ATCAACCTCCGAAACCATAG+AGG	0.481091	3.4:+57889641	None:intergenic
CTTGCCAATCGACGAATAGC+AGG	0.484591	3.4:+57889835	None:intergenic
GAATATCTTCAATACACCTC+TGG	0.487673	3.4:+57889768	None:intergenic
GGTAAGGGACTCGGAAAGGG+AGG	0.488695	3.4:-57889910	MS.gene04433:CDS
AGGCCGTGGAAAGGGAGGTA+AGG	0.492819	3.4:-57889926	MS.gene04433:CDS
GGAGGTAAGGGACTCGGAAA+GGG	0.499726	3.4:-57889913	MS.gene04433:CDS
CTCTTGAAAATGTCAGGCCG+TGG	0.503870	3.4:-57889940	None:intergenic
GTCCCTTACCTCCCTTTCCA+CGG	0.513750	3.4:+57889923	None:intergenic
AAAATGTCAGGCCGTGGAAA+GGG	0.515750	3.4:-57889934	MS.gene04433:CDS
GGGAGGTAAGGGACTCGGAA+AGG	0.518226	3.4:-57889914	MS.gene04433:CDS
TTACATACACTGAGCATGCT+AGG	0.520723	3.4:-57889720	MS.gene04433:CDS
GGTTCTTCGTGATAACATCC+AGG	0.522451	3.4:-57889869	MS.gene04433:CDS
AGGTGCAAAGAGGCATAGAA+AGG	0.530485	3.4:-57889890	MS.gene04433:CDS
CAATCGACGAATAGCAGGCT+TGG	0.531595	3.4:+57889840	None:intergenic
CAAGCCTGCTATTCGTCGAT+TGG	0.532828	3.4:-57889839	MS.gene04433:CDS
GGAAAGGGAGGTAAGGGACT+CGG	0.535187	3.4:-57889919	MS.gene04433:CDS
GAGGAAGACGGTGACAGCCA+TGG	0.546349	3.4:-57889698	MS.gene04433:CDS
GAAAATGTCAGGCCGTGGAA+AGG	0.550606	3.4:-57889935	None:intergenic
GGCCGTGGAAAGGGAGGTAA+GGG	0.552535	3.4:-57889925	MS.gene04433:CDS
GGTGGTGTGAAGAGAATCAG+TGG	0.556654	3.4:-57889808	MS.gene04433:CDS
GTCTATGCTTTGAAGAGACA+AGG	0.570048	3.4:-57889670	MS.gene04433:CDS
CGTCGATTGGCAAGAAGAGG+TGG	0.593946	3.4:-57889826	MS.gene04433:CDS
TAGCAGGCTTGGTGATACCC+TGG	0.594313	3.4:+57889851	None:intergenic
AATCAGTGGATTGATCTATG+AGG	0.599604	3.4:-57889794	MS.gene04433:CDS
ATTCGTCGATTGGCAAGAAG+AGG	0.629429	3.4:-57889829	MS.gene04433:CDS
CAAAGCATAGACAACATCCA+TGG	0.629842	3.4:+57889681	None:intergenic
AGAACCCTCTATGGTTTCGG+AGG	0.630900	3.4:-57889646	MS.gene04433:CDS
GTTCTTCGTGATAACATCCA+GGG	0.632029	3.4:-57889868	MS.gene04433:CDS
ATGTCAGGCCGTGGAAAGGG+AGG	0.634999	3.4:-57889931	MS.gene04433:CDS
TCAACCTCCGAAACCATAGA+GGG	0.666233	3.4:+57889642	None:intergenic
TGAGCATGCTAGGAGGAAGA+CGG	0.671956	3.4:-57889710	MS.gene04433:CDS
TCGGAAAGGGAGGTGCAAAG+AGG	0.683014	3.4:-57889900	MS.gene04433:CDS
CAGTGTATGTAACTGCATCA+CGG	0.689582	3.4:+57889731	None:intergenic
TTGATCTATGAGGAAACCAG+AGG	0.700822	3.4:-57889784	MS.gene04433:CDS
CATACACTGAGCATGCTAGG+AGG	0.707859	3.4:-57889717	MS.gene04433:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGGTGTATTGAAGATATTCT+TGG	-	chr3.4:57889809-57889828	MS.gene04433:CDS	30.0%
	GAATATCTTCAATACACCTC+TGG	+	chr3.4:57889808-57889827	None:intergenic	35.0%
!!	AATCAGTGGATTGATCTATG+AGG	-	chr3.4:57889779-57889798	MS.gene04433:CDS	35.0%
	CAAAGCATAGACAACATCCA+TGG	+	chr3.4:57889895-57889914	None:intergenic	40.0%
	CAGTGTATGTAACTGCATCA+CGG	+	chr3.4:57889845-57889864	None:intergenic	40.0%
	GTTCTTCGTGATAACATCCA+GGG	-	chr3.4:57889705-57889724	MS.gene04433:CDS	40.0%
	TTACATACACTGAGCATGCT+AGG	-	chr3.4:57889853-57889872	MS.gene04433:CDS	40.0%
	TTGATCTATGAGGAAACCAG+AGG	-	chr3.4:57889789-57889808	MS.gene04433:CDS	40.0%
!!	GTCTATGCTTTGAAGAGACA+AGG	-	chr3.4:57889903-57889922	MS.gene04433:CDS	40.0%
	AGACAAGGAAGAACCCTCTA+TGG	-	chr3.4:57889918-57889937	MS.gene04433:CDS	45.0%
	GGAAGAACCCTCTATGGTTT+CGG	-	chr3.4:57889924-57889943	MS.gene04433:CDS	45.0%
	GGTTCTTCGTGATAACATCC+AGG	-	chr3.4:57889704-57889723	MS.gene04433:CDS	45.0%
	TCAACCTCCGAAACCATAGA+GGG	+	chr3.4:57889934-57889953	None:intergenic	45.0%
!	AGGTGCAAAGAGGCATAGAA+AGG	-	chr3.4:57889683-57889702	MS.gene04433:CDS	45.0%
!	ATTCGTCGATTGGCAAGAAG+AGG	-	chr3.4:57889744-57889763	MS.gene04433:CDS	45.0%
	AGAACCCTCTATGGTTTCGG+AGG	-	chr3.4:57889927-57889946	MS.gene04433:CDS	50.0%
	CAAGCCTGCTATTCGTCGAT+TGG	-	chr3.4:57889734-57889753	MS.gene04433:CDS	50.0%
	CAATCGACGAATAGCAGGCT+TGG	+	chr3.4:57889736-57889755	None:intergenic	50.0%
	CATACACTGAGCATGCTAGG+AGG	-	chr3.4:57889856-57889875	MS.gene04433:CDS	50.0%
	CTTGCCAATCGACGAATAGC+AGG	+	chr3.4:57889741-57889760	None:intergenic	50.0%
	GGTGGTGTGAAGAGAATCAG+TGG	-	chr3.4:57889765-57889784	MS.gene04433:CDS	50.0%
	TGAGCATGCTAGGAGGAAGA+CGG	-	chr3.4:57889863-57889882	MS.gene04433:CDS	50.0%
	GGAAAGGGAGGTAAGGGACT+CGG	-	chr3.4:57889654-57889673	MS.gene04433:CDS	55.0%
	GGAGGTAAGGGACTCGGAAA+GGG	-	chr3.4:57889660-57889679	MS.gene04433:CDS	55.0%
	GTCCCTTACCTCCCTTTCCA+CGG	+	chr3.4:57889653-57889672	None:intergenic	55.0%
!	CGTCGATTGGCAAGAAGAGG+TGG	-	chr3.4:57889747-57889766	MS.gene04433:CDS	55.0%
!	TCGGAAAGGGAGGTGCAAAG+AGG	-	chr3.4:57889673-57889692	MS.gene04433:CDS	55.0%
!!	TAGCAGGCTTGGTGATACCC+TGG	+	chr3.4:57889725-57889744	None:intergenic	55.0%
	AGGCCGTGGAAAGGGAGGTA+AGG	-	chr3.4:57889647-57889666	MS.gene04433:CDS	60.0%
	ATGTCAGGCCGTGGAAAGGG+AGG	-	chr3.4:57889642-57889661	MS.gene04433:CDS	60.0%
	GAGGAAGACGGTGACAGCCA+TGG	-	chr3.4:57889875-57889894	MS.gene04433:CDS	60.0%
	GGCCGTGGAAAGGGAGGTAA+GGG	-	chr3.4:57889648-57889667	MS.gene04433:CDS	60.0%
	GGGAGGTAAGGGACTCGGAA+AGG	-	chr3.4:57889659-57889678	MS.gene04433:CDS	60.0%
	GGTAAGGGACTCGGAAAGGG+AGG	-	chr3.4:57889663-57889682	MS.gene04433:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	57889642	57889953	57889642	ID=MS.gene04433
chr3.4	mRNA	57889642	57889953	57889642	ID=MS.gene04433.t1;Parent=MS.gene04433
chr3.4	exon	57889642	57889953	57889642	ID=MS.gene04433.t1.exon1;Parent=MS.gene04433.t1
chr3.4	CDS	57889642	57889953	57889642	ID=cds.MS.gene04433.t1;Parent=MS.gene04433.t1

Gene Sequence	>MS.gene04433 ATGTCAGGCCGTGGAAAGGGAGGTAAGGGACTCGGAAAGGGAGGTGCAAAGAGGCATAGAAAGGTTCTTCGTGATAACATCCAGGGTATCACCAAGCCTGCTATTCGTCGATTGGCAAGAAGAGGTGGTGTGAAGAGAATCAGTGGATTGATCTATGAGGAAACCAGAGGTGTATTGAAGATATTCTTGGAAAATGTCATCCGTGATGCAGTTACATACACTGAGCATGCTAGGAGGAAGACGGTGACAGCCATGGATGTTGTCTATGCTTTGAAGAGACAAGGAAGAACCCTCTATGGTTTCGGAGGTTGA
Protein sequence	>MS.gene04433.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG